FastQCFastQC Report
Thu 26 May 2016
SRR936343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936343_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3259165
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68270.20947083071891112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53640.16458203251446304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67050.038.8862271
GTATCAA105700.037.8110161
TCAACGC129900.030.4187974
ATCAACG130600.030.2194353
CAACGCA133450.029.6451515
AACGCAG137250.028.8589366
TATCAAC138950.028.584222
GTACATG169400.026.935141
TACATGG166400.026.8631572
ACATGGG170850.025.1269533
ACGCAGA163200.024.0666897
CGCAGAG165900.023.6464168
CATGGGA83600.022.185884
CATGGGG85200.022.1589764
GAGTACA138650.021.0699041
AGTACAT128250.020.9050182
GCAGAGT185050.020.8404779
ATGGGAT32400.019.7649155
AACGCGT1456.2056206E-419.6286077
GTATACG1707.858754E-519.5912971
Produced by FastQC (version 0.11.5)