##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936342_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 93743 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.140671836830485 33.0 31.0 34.0 30.0 34.0 2 32.21849098065989 33.0 31.0 34.0 30.0 34.0 3 32.28582400819261 34.0 31.0 34.0 30.0 34.0 4 35.8394973491354 37.0 35.0 37.0 35.0 37.0 5 35.83507035191961 37.0 35.0 37.0 35.0 37.0 6 35.94930821501339 37.0 35.0 37.0 35.0 37.0 7 35.886764878444254 37.0 35.0 37.0 35.0 37.0 8 35.90402483385426 37.0 35.0 37.0 35.0 37.0 9 37.49826653723478 39.0 37.0 39.0 34.0 39.0 10-11 37.53966696179981 39.0 37.0 39.0 35.0 39.0 12-13 37.4871243719531 39.0 37.0 39.0 34.5 39.0 14-15 38.49490628633605 40.0 38.0 41.0 33.5 41.0 16-17 38.41393490713973 40.0 38.0 41.0 33.5 41.0 18-19 38.546862165708376 40.0 38.0 41.0 34.0 41.0 20-21 38.52535122622489 40.0 38.0 41.0 34.0 41.0 22-23 38.401533981203926 40.0 37.5 41.0 34.0 41.0 24-25 38.35957884855402 40.0 37.0 41.0 34.0 41.0 26-27 38.05619086225104 40.0 36.5 41.0 33.5 41.0 28-29 37.78749346617881 40.0 36.0 41.0 33.0 41.0 30-31 37.33531570357253 39.0 35.0 41.0 33.0 41.0 32-33 36.819138495674345 39.0 35.0 41.0 33.0 41.0 34-35 36.45912761486191 38.0 35.0 41.0 32.0 41.0 36-37 36.10329304588076 38.0 35.0 41.0 31.0 41.0 38-39 35.69264905112915 38.0 35.0 41.0 30.5 41.0 40-41 35.29522737697748 38.0 35.0 41.0 29.5 41.0 42-43 34.84262291584439 38.0 35.0 41.0 26.0 41.0 44-45 34.21411198702837 38.0 35.0 41.0 21.0 41.0 46-47 33.49895992234087 38.0 34.5 41.0 5.5 41.0 48-49 32.84749261278175 38.0 33.5 41.0 2.0 41.0 50-51 32.34830333998272 37.0 33.0 40.5 2.0 41.0 52-53 31.995375654715552 37.0 33.0 40.0 2.0 41.0 54-55 31.66230011840884 37.0 33.0 40.0 2.0 41.0 56-57 31.147568351770268 36.5 32.0 40.0 2.0 41.0 58-59 30.671367462103838 36.0 31.0 40.0 2.0 41.0 60-61 30.12735350906201 35.0 30.0 40.0 2.0 41.0 62-63 29.687464664028248 35.0 29.0 39.5 2.0 41.0 64-65 29.167111144298772 35.0 28.0 39.0 2.0 41.0 66-67 28.604823826845738 35.0 26.0 38.5 2.0 40.5 68-69 28.092172215525423 35.0 25.5 37.5 2.0 40.0 70-71 27.578885890146466 35.0 24.0 37.0 2.0 39.0 72-73 27.12546003434923 35.0 21.5 36.0 2.0 39.0 74-75 26.74043395240178 34.5 20.0 36.0 2.0 38.5 76-77 26.077392445302582 34.0 16.5 35.0 2.0 37.0 78-79 26.064260798139593 34.0 12.0 35.0 2.0 37.0 80-81 25.87457196804028 34.0 8.5 35.0 2.0 36.5 82-83 25.59031074320216 34.0 3.5 35.0 2.0 36.0 84-85 25.31464749367953 34.0 2.0 35.0 2.0 36.0 86-87 25.011270174839723 34.0 2.0 35.0 2.0 35.0 88-89 24.555417471171182 33.0 2.0 35.0 2.0 35.0 90-91 24.326712394525458 33.0 2.0 35.0 2.0 35.0 92-93 24.006949852255634 33.0 2.0 35.0 2.0 35.0 94-95 23.856986655003574 33.0 2.0 35.0 2.0 35.0 96-97 23.609474840788113 33.0 2.0 35.0 2.0 35.0 98-99 23.254963037240117 33.0 2.0 35.0 2.0 35.0 100-101 21.032450422964914 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.42983539520558267 1101 2 -0.2769125188476167 1101 3 -0.116548073499672 1101 4 0.27613550523847863 1101 5 -0.2690726607957501 1101 6 0.2973817120416342 1101 7 0.24835739651530275 1101 8 0.17511405713615602 1101 9 0.1545896049562998 1101 10-11 0.04657009705811532 1101 12-13 0.4582969468894973 1101 14-15 -0.7201971745301279 1101 16-17 -0.36085209392539497 1101 18-19 -0.007842510447105155 1101 20-21 0.7334467179477571 1101 22-23 0.5404617144152084 1101 24-25 0.41062248333273743 1101 26-27 0.7926363019713776 1101 28-29 0.7415013366938084 1101 30-31 0.45335876895774163 1101 32-33 0.7149242427379789 1101 34-35 0.46973755629169744 1101 36-37 1.0813460279669442 1101 38-39 0.8433422238529289 1101 40-41 0.6729709641091475 1101 42-43 0.5572101589456153 1101 44-45 -0.22359035411763983 1101 46-47 -0.5710791336483254 1101 48-49 -1.3081058565404717 1101 50-51 -1.512970082753398 1101 52-53 -1.5555267887386321 1101 54-55 -1.7503899120539828 1101 56-57 -1.6958349128427415 1101 58-59 -1.5953161363931905 1101 60-61 -0.9115933434043235 1101 62-63 -0.940378443665459 1101 64-65 -0.433452691024403 1101 66-67 -0.0389104477450104 1101 68-69 0.004232151815156016 1101 70-71 -0.03602337490974605 1101 72-73 -0.6087469029816361 1101 74-75 -0.4490784770863918 1101 76-77 -0.7283737523621916 1101 78-79 -1.106286485643711 1101 80-81 -0.4011735377125838 1101 82-83 -0.07701176902532936 1101 84-85 -0.4060226064564887 1101 86-87 -0.4965433806083084 1101 88-89 -1.0370585043862057 1101 90-91 -0.153024816724443 1101 92-93 -0.06655461514736771 1101 94-95 5.076927499914063E-4 1101 96-97 -0.4063549390507184 1101 98-99 -0.11498855518915363 1101 100-101 0.235502000834785 1102 1 -0.05927983965002426 1102 2 0.10919859226349615 1102 3 0.261229704278108 1102 4 0.07113550523848033 1102 5 0.3337051169820313 1102 6 0.19293726759718766 1102 7 0.16113517429307933 1102 8 0.07678072380281975 1102 9 -0.007077061710369037 1102 10-11 -0.09120768071966978 1102 12-13 -0.028091941999392134 1102 14-15 0.5792472699143119 1102 16-17 0.30775901718571674 1102 18-19 -0.07200917711377741 1102 20-21 -0.3454421709411335 1102 22-23 -0.308149396695903 1102 24-25 0.008400261110516283 1102 26-27 0.17652519086026786 1102 28-29 0.290945781138241 1102 30-31 0.3386365467355148 1102 32-33 0.26186868718242096 1102 34-35 0.6139042229583609 1102 36-37 0.5432904724113925 1102 38-39 0.8033422238529297 1102 40-41 1.1488042974424744 1102 42-43 0.8160990478345127 1102 44-45 0.6394652014379147 1102 46-47 0.3611430885738969 1102 48-49 0.05911636568174572 1102 50-51 0.39675213946881627 1102 52-53 0.5564176557058076 1102 54-55 0.6690545323904651 1102 56-57 0.732220642712818 1102 58-59 0.7094060858290305 1102 60-61 0.14896221215123262 1102 62-63 0.1323993341123213 1102 64-65 0.012658420086708588 1102 66-67 0.8869228855883229 1102 68-69 0.2233988184818223 1102 70-71 0.6798099584235864 1102 72-73 0.31236420812947685 1102 74-75 0.3089770784691659 1102 76-77 0.30718180319336597 1102 78-79 0.6123246254674015 1102 80-81 0.12799312895408477 1102 82-83 0.3246548976413344 1102 84-85 0.52758850465462 1102 86-87 0.14651217494724733 1102 88-89 0.03238594005824069 1102 90-91 -0.044413705613333576 1102 92-93 -0.015721281814034427 1102 94-95 -0.4525478628055666 1102 96-97 -0.051077161272939264 1102 98-99 -0.5458218885224895 1102 100-101 0.4874464452792253 1103 1 -0.6133149273693235 1103 2 -0.6986961445786086 1103 3 -0.799296611511366 1103 4 -0.9014960737088913 1103 5 -1.0373475145969167 1103 6 -0.5067118552098293 1103 7 0.09411763043343058 1103 8 0.08958774134668346 1103 9 0.11730890320190923 1103 10-11 -0.12375154036879366 1103 12-13 -0.0018638718239571972 1103 14-15 -0.06084044938392452 1103 16-17 -0.20259186000726714 1103 18-19 -0.07911444027166681 1103 20-21 -0.12474041655516999 1103 22-23 0.05764007698830653 1103 24-25 -0.3401085108193058 1103 26-27 -0.31408884422745587 1103 28-29 -0.6583524644757901 1103 30-31 0.2563558449811296 1103 32-33 0.1275704415683876 1103 34-35 -0.1322361279188229 1103 36-37 0.46109748995525024 1103 38-39 0.46614924139679204 1103 40-41 0.8319621921793185 1103 42-43 0.5886429074836315 1103 44-45 0.8308687102098418 1103 46-47 0.45377466752126594 1103 48-49 0.8154321551554347 1103 50-51 0.7410503850828576 1103 52-53 0.02159309430230394 1103 54-55 0.7883527780044979 1103 56-57 0.0669574848180794 1103 58-59 0.41966924372376724 1103 60-61 0.1571201068880761 1103 62-63 -0.5507585606245229 1103 64-65 0.2061671920165331 1103 66-67 0.4512211312023595 1103 68-69 0.6719953097098923 1103 70-71 0.5308625900025312 1103 72-73 1.0023642081294746 1103 74-75 0.9781876047849529 1103 76-77 0.6561291716144204 1103 78-79 0.6024123447656464 1103 80-81 0.8696597956207519 1103 82-83 1.1664092836062494 1103 84-85 1.0153955221984816 1103 86-87 0.9000209468770706 1103 88-89 0.9578245365494702 1103 90-91 0.976726645263863 1103 92-93 1.4433138059052624 1103 94-95 0.9679784529839068 1103 96-97 0.6707649439902177 1103 98-99 1.0154939009511956 1103 100-101 -0.5404482915628783 1104 1 0.09987627849343284 1104 2 0.4940087188457767 1104 3 0.30679932453126924 1104 4 0.11316082169417285 1104 5 0.04509752204531736 1104 6 0.3613760861625863 1104 7 0.3842575371622772 1104 8 0.4804938039715978 1104 9 0.6867626007368912 1104 10-11 0.6126741758204233 1104 12-13 0.7310641761440664 1104 14-15 0.27435275514638846 1104 16-17 0.48944678089879545 1104 18-19 0.7171047469368546 1104 20-21 0.7886506560630835 1104 22-23 0.7260278184939679 1104 24-25 0.9521133412792935 1104 26-27 1.168339536851832 1104 28-29 1.1316208866234803 1104 30-31 0.8891006817566165 1104 32-33 1.0741893622879104 1104 34-35 0.9578704676841028 1104 36-37 0.651054185491553 1104 38-39 0.708321126806517 1104 40-41 1.2776650569361507 1104 42-43 0.5737361786361959 1104 44-45 -0.2885516761992193 1104 46-47 -1.079869569653951 1104 48-49 -1.0051874317866023 1104 50-51 -0.9049356242442599 1104 52-53 -1.8624009096950296 1104 54-55 -1.1946585477783138 1104 56-57 -0.5936021420973105 1104 58-59 -0.29160657239881616 1104 60-61 -0.39559474987408194 1104 62-63 -0.3065880076598333 1104 64-65 -0.44523187527193997 1104 66-67 -1.0365370300234886 1104 68-69 -1.5756729114759835 1104 70-71 -1.2098102947409721 1104 72-73 -1.1906737665540668 1104 74-75 -1.0145672253283031 1104 76-77 -1.2740840195914451 1104 78-79 -1.1789833914102381 1104 80-81 -1.0623022296957032 1104 82-83 -0.9615054399114058 1104 84-85 -1.4054072759360992 1104 86-87 -1.1121376140822896 1104 88-89 -0.8874452835704538 1104 90-91 -0.7035698237567907 1104 92-93 -0.9012065138815473 1104 94-95 -0.6697630526789844 1104 96-97 -0.8303176676020563 1104 98-99 -0.8984801163705889 1104 100-101 -0.46166747877140324 1105 1 -0.3244830916825485 1105 2 -0.33958189554137874 1105 3 0.1368394603756684 1105 4 0.3950379442628673 1105 5 0.2678514584454419 1105 6 0.17814051962970723 1105 7 0.3848750116914559 1105 8 0.4159677156727426 1105 9 0.37308553991564963 1105 10-11 0.35009313228846395 1105 12-13 0.30060724499247016 1105 14-15 0.5606293837354528 1105 16-17 0.6003606432019737 1105 18-19 0.0770152131301245 1105 20-21 -0.18796249614437954 1105 22-23 0.07111889598701993 1105 24-25 -0.06972982019029672 1105 26-27 0.28619998760822796 1105 28-29 0.45525472422767876 1105 30-31 0.6828641890119371 1105 32-33 0.024714215637708037 1105 34-35 -0.13544537053757466 1105 36-37 0.8758920984276486 1105 38-39 0.8596023864545614 1105 40-41 1.1358774681741792 1105 42-43 1.5477250640946707 1105 44-45 1.5458879656655569 1105 46-47 2.0596796739397476 1105 48-49 2.5757017315354034 1105 50-51 2.758540757355 1105 52-53 2.049994891478171 1105 54-55 2.656452906374202 1105 56-57 2.928318203688427 1105 58-59 2.7625768175363525 1105 60-61 1.8787183097122089 1105 62-63 2.2367895780147578 1105 64-65 2.085178745289962 1105 66-67 2.066272479084258 1105 68-69 2.6216102005956436 1105 70-71 2.8963953242772433 1105 72-73 2.4169983544709375 1105 74-75 2.5287331760301406 1105 76-77 2.5284013153884892 1105 78-79 2.2370400726218698 1105 80-81 2.6105134541573385 1105 82-83 2.846768718779547 1105 84-85 2.9972633014025902 1105 86-87 3.1814715245407434 1105 88-89 3.219377809976937 1105 90-91 3.1476188147118727 1105 92-93 3.0538722141209256 1105 94-95 3.458671649389558 1105 96-97 3.827215521653887 1105 98-99 3.8763732334287333 1105 100-101 3.775983030645083 1106 1 0.10464172897742685 1106 2 0.19155153343997 1106 3 0.20240617486633994 1106 4 0.16290021112083508 1106 5 0.25135217580555747 1106 6 0.30195687544032523 1106 7 0.24427242919504266 1106 8 0.4265846453714488 1106 9 0.23449156574061192 1106 10-11 0.31192957418229383 1106 12-13 0.8181825678045342 1106 14-15 0.5972864856005984 1106 16-17 0.2387394093425783 1106 18-19 0.023284940533280007 1106 20-21 0.41024410356867236 1106 22-23 0.003615309186450588 1106 24-25 -0.2429722879090903 1106 26-27 0.0016232300759497775 1106 28-29 0.03839676153040017 1106 30-31 0.06765615457865692 1106 32-33 -0.5506803324254221 1106 34-35 -0.23393891429653735 1106 36-37 -0.04455266484351483 1106 38-39 0.42373438071567904 1106 40-41 -0.21943099667516464 1106 42-43 0.5529617929325497 1106 44-45 0.3567201033987004 1106 46-47 0.7776136768091888 1106 48-49 0.9979398950935092 1106 50-51 0.8104776296649021 1106 52-53 1.309750989039152 1106 54-55 0.882780022586541 1106 56-57 1.2839853485951664 1106 58-59 1.2717590270054977 1106 60-61 1.5277857415629974 1106 62-63 1.5888699223476124 1106 64-65 1.701678027929848 1106 66-67 1.950060140490283 1106 68-69 1.6226145047563314 1106 70-71 1.977457017247115 1106 72-73 2.5817759728353558 1106 74-75 2.0513300196456363 1106 76-77 1.9389465090757163 1106 78-79 1.864873645075246 1106 80-81 2.0181892073854577 1106 82-83 1.9979882309746735 1106 84-85 1.8126865438703064 1106 86-87 1.4778847239668522 1106 88-89 1.106895743979809 1106 90-91 1.5093117845827457 1106 92-93 1.5591806789702787 1106 94-95 1.4250991960179604 1106 96-97 1.0500993093152964 1106 98-99 1.2612369350069237 1106 100-101 1.4792111511615786 1107 1 -0.32644033347718704 1107 2 0.030186246584484877 1107 3 -0.17827646856139978 1107 4 0.2622466163495929 1107 5 0.07197672192030069 1107 6 0.1800977614243493 1107 7 -0.35195124546000756 1107 8 -0.1844538440984138 1107 9 0.5573673827340784 1107 10-11 0.22558244273712802 1107 12-13 0.13190805800060446 1107 14-15 0.4449262822599991 1107 16-17 0.49022815298818756 1107 18-19 0.5731760080714139 1107 20-21 0.4340640018983777 1107 22-23 0.4980234428102648 1107 24-25 0.4846965574068136 1107 26-27 0.08738938839113075 1107 28-29 0.06403220089133299 1107 30-31 0.4433279047602099 1107 32-33 0.04902918100957976 1107 34-35 0.5677313834521911 1107 36-37 -0.09843792265034068 1107 38-39 -0.07641086256682428 1107 40-41 0.3959647912696411 1107 42-43 -0.3347651496963522 1107 44-45 -0.4064607244880136 1107 46-47 -0.1914495040186992 1107 48-49 0.15640031629903461 1107 50-51 0.7671225098391901 1107 52-53 0.31098555694038055 1107 54-55 0.9949804583163875 1107 56-57 0.9808626180214546 1107 58-59 1.2326159623722397 1107 60-61 1.1825424590648126 1107 62-63 0.7185721736184902 1107 64-65 1.8983374324323883 1107 66-67 1.5575401695389388 1107 68-69 0.7858679542842921 1107 70-71 1.1684519337322286 1107 72-73 0.7832284056603385 1107 74-75 0.7245326340247225 1107 76-77 0.46915711183534015 1107 78-79 0.9454110452204851 1107 80-81 1.1983634993244543 1107 82-83 0.7177413173944238 1107 84-85 0.10092183798795418 1107 86-87 0.04898131074971701 1107 88-89 0.7113982857372498 1107 90-91 0.284475183275557 1107 92-93 0.23514291571682833 1107 94-95 0.6123904087993708 1107 96-97 -0.5058919760877565 1107 98-99 -0.4845873206212552 1107 100-101 0.8180637292298485 1108 1 -0.6831878856270386 1108 2 -0.16436462612730907 1108 3 -0.005436962388557731 1108 4 0.08216998799709785 1108 5 -0.10767419336279715 1108 6 -0.23326962895453818 1108 7 -0.03564643490232555 1108 8 -0.37517329918568265 1108 9 -0.0020195904460038605 1108 10-11 -0.3576444623288566 1108 12-13 -0.02901148222927219 1108 14-15 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0.25749196054227497 2316 58-59 0.15064639590655204 2316 60-61 0.7650862431589864 2316 62-63 1.239531117058057 2316 64-65 1.2619607456681017 2316 66-67 1.3084732731852213 2316 68-69 1.0454143223577894 2316 70-71 1.3094223615243585 2316 72-73 1.38027118487366 2316 74-75 1.482930566841258 2316 76-77 1.745786454356157 2316 78-79 1.5062781138394925 2316 80-81 1.7233419661633853 2316 82-83 1.8716316418273813 2316 84-85 2.0621621480654753 2316 86-87 1.533333880373604 2316 88-89 1.7610681106008776 2316 90-91 1.3180669145417063 2316 92-93 1.204278718185968 2316 94-95 1.7942738426207896 2316 96-97 1.6926437689596163 2316 98-99 2.137433925431001 2316 100-101 1.7550433445040348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 8.0 6 34.0 7 56.0 8 105.0 9 206.0 10 314.0 11 514.0 12 793.0 13 1110.0 14 1388.0 15 1743.0 16 2104.0 17 2582.0 18 2376.0 19 1542.0 20 1346.0 21 1412.0 22 1319.0 23 1305.0 24 1249.0 25 1316.0 26 1225.0 27 1146.0 28 1361.0 29 1406.0 30 1737.0 31 2074.0 32 2571.0 33 3245.0 34 4452.0 35 8989.0 36 9485.0 37 15199.0 38 15525.0 39 2503.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.05026737967915 21.21818181818182 14.324064171122993 16.407486631016045 2 15.223676770263205 26.1029137193398 36.08381610814156 22.58959340225544 3 21.35199428223974 32.29254450999008 29.988372465144064 16.367088742626116 4 11.31924517030605 21.215450753656274 35.43517916004395 32.03012491599373 5 9.604983838793297 43.05494810279168 31.90638234321496 15.433685715200069 6 25.71285322637424 39.43014411742743 15.643834739660562 19.213167916537767 7 20.300182413620217 40.84784997279797 21.009568714464013 17.8423988991178 8 26.77746605079846 31.264201060345837 20.803686675271756 21.15464621358395 9 25.496303724011394 18.536850751522778 21.197316066266282 34.76952945819954 10-11 23.315340878785616 29.73875382695241 23.952721803227973 22.993183491033996 12-13 27.16202809809799 28.966429493402174 26.19768942747725 17.673852981022584 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30.376134751394773 35.076752397512344 17.95974099399422 40-41 15.509507427260846 31.064351814811854 35.01026748806571 18.41587326986159 42-43 16.50647009249085 30.895891784636063 34.409370699495405 18.188267423377678 44-45 16.844732953909013 30.095958437568342 34.37114952767539 18.68815908084725 46-47 17.14579876985122 30.73984967219149 33.85523079959671 18.259120758360584 48-49 16.878274136588175 30.015363769244725 33.749613238447836 19.356748855719268 50-51 15.66232283737578 30.806159951778696 34.12670894921293 19.40480826163259 52-53 16.43124619931081 31.505979750994843 31.712417184982876 20.350356864711465 54-55 16.246633063978454 30.289889857855297 31.799877323519215 21.663599754647038 56-57 17.514054982451274 29.973650241628352 30.9481645846446 21.564130191275776 58-59 17.95779906552026 29.754005675150946 31.22773143308229 21.060463826246504 60-61 17.39615113823046 31.107721192208405 31.03144801689744 20.464679652663694 62-63 15.954257917924538 31.136724875457368 30.938310060484515 21.970707146133577 64-65 15.940560261142284 31.385078193339165 30.339122271767188 22.33523927375136 66-67 17.45623673234268 29.95210309036408 29.016566570303915 23.575093606989324 68-69 17.51490777978089 29.3600588843967 29.77982356015916 23.34520977566325 70-71 18.34493426140758 29.362348987919034 29.662639677840897 22.630077072832496 72-73 18.1534030296565 28.461702581608705 30.575528056326007 22.80936633240879 74-75 17.499439958183544 30.09344697737431 29.391527900749924 23.015585163692222 76-77 17.560404800324868 28.719129700674312 30.003633371448114 23.716832127552713 78-79 17.495862032142668 29.16973677185114 30.28618719632655 23.048213999679643 80-81 17.80961473840295 29.23571737170837 29.32959254972451 23.625075340164177 82-83 17.26955612685747 28.872555817501038 29.92916804454733 23.928720011094164 84-85 16.98971645580423 28.988607027799706 29.440378912333852 24.581297604062215 86-87 17.538012913976257 29.173409385865124 28.61796954694268 24.67060815321594 88-89 17.591494976693788 28.894336242363128 28.246375334574186 25.2677934463689 90-91 17.806658034691978 28.37048292688147 28.433082578035545 25.389776460391005 92-93 18.061223943985922 28.863939064898663 28.331719737046207 24.743117254069205 94-95 17.580635697864828 28.340774166706673 28.868833309330643 25.209756826097856 96-97 17.14547840279926 28.79911242919169 28.98740118840611 25.068007979602942 98-99 18.149329521330504 29.096747351689267 27.222394094240393 25.53152903273984 100-101 17.05096407712617 29.07912633010641 27.085900205349763 26.78400938741766 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2599.0 1 1480.0 2 537.0 3 674.0 4 430.5 5 316.0 6 587.5 7 941.0 8 1169.0 9 1030.5 10 591.0 11 322.0 12 284.0 13 265.0 14 248.0 15 233.5 16 216.0 17 217.0 18 245.0 19 288.5 20 304.0 21 317.5 22 390.0 23 493.0 24 572.5 25 643.0 26 758.5 27 909.0 28 1039.5 29 1195.0 30 1421.5 31 1564.5 32 1755.5 33 2094.5 34 2261.0 35 2271.5 36 2415.5 37 2578.5 38 2452.0 39 2245.0 40 2177.5 41 2113.5 42 1999.5 43 1899.0 44 1882.0 45 1929.0 46 1965.0 47 2787.5 48 4479.0 49 5122.5 50 5685.0 51 5404.5 52 3488.5 53 2193.5 54 1624.5 55 1367.0 56 1246.0 57 1195.5 58 1136.5 59 980.5 60 782.5 61 677.5 62 522.0 63 338.5 64 222.5 65 180.5 66 178.0 67 183.0 68 162.5 69 126.5 70 105.0 71 122.5 72 120.0 73 86.5 74 71.5 75 64.0 76 34.5 77 16.0 78 8.0 79 1.5 80 1.5 81 1.5 82 0.5 83 0.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2592193550451767 2 0.014934448438816764 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.333731585291702E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003200238951175021 26-27 0.002666865792645851 28-29 0.002666865792645851 30-31 0.0016001194755875105 32-33 0.003200238951175021 34-35 0.002666865792645851 36-37 0.0 38-39 0.0 40-41 5.333731585291702E-4 42-43 0.004266985268233362 44-45 0.004800358426762532 46-47 0.015467821597345936 48-49 0.017067941072933447 50-51 0.009067343694995893 52-53 0.010667463170583404 54-55 5.333731585291702E-4 56-57 0.004266985268233362 58-59 0.0010667463170583404 60-61 0.0010667463170583404 62-63 0.0 64-65 0.0010667463170583404 66-67 0.0 68-69 0.0 70-71 5.333731585291702E-4 72-73 0.003200238951175021 74-75 0.0 76-77 0.1770798886316845 78-79 0.10454113907171735 80-81 0.0016001194755875105 82-83 0.0 84-85 0.0010667463170583404 86-87 0.13174317015670503 88-89 0.5627086822482745 90-91 0.31148992458103536 92-93 0.2842878934960477 94-95 0.003733612109704191 96-97 0.004266985268233362 98-99 0.004266985268233362 100-101 5.333731585291702E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 93743.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.17138346329859 #Duplication Level Percentage of deduplicated Percentage of total 1 73.71936394377218 28.139701097681964 2 10.80401307883632 8.248082523495087 3 4.876617388145209 5.584416969800412 4 2.727552189587234 4.16457762179576 5 1.6544169018807815 3.1575690984926874 6 1.3246513707626526 3.03382652571392 7 0.8886901601319062 2.3745773017718657 8 0.6902719168320152 2.1078907225072805 9 0.5505407595785708 1.8913412201444375 >10 2.5123662074169295 16.458828925893133 >50 0.12296341838303104 3.216240145930896 >100 0.09222256378727328 7.218672327533788 >500 0.0223569851605511 5.240924655707626 >1k 0.013973115725344439 9.163350863531143 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2827 3.0156918383239284 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2020 2.1548275604578477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1507 1.6075866998069188 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1194 1.2736951025676584 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1042 1.1115496623747907 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 942 1.0048750306689567 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 605 0.645381521820296 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 595 0.6347140586497125 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 588 0.6272468344303042 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 565 0.6027116691379623 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 551 0.5877772206991455 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 548 0.5845769817479706 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 519 0.5536413385532787 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 433 0.46190115528626136 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 295 0.3146901635322104 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 288 0.30722293931280203 RNA PCR Primer, Index 17 (95% over 21bp) GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 281 0.29975571509339366 No Hit TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 261 0.2784207887522268 RNA PCR Primer, Index 17 (96% over 28bp) CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 259 0.27628729611811015 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 258 0.2752205498010518 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 256 0.27308705716693515 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 253 0.2698868182157601 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 243 0.2592193550451767 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 243 0.2592193550451767 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 239 0.25495236977694336 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 232 0.24748514555753498 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 213 0.2272169655334265 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 204 0.21761624867990145 RNA PCR Primer, Index 17 (95% over 23bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 195 0.20801553182637636 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 191 0.203748546558143 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 189 0.20161505392402632 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 183 0.19521457602167627 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 182 0.19414782970461794 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 178 0.18988084443638456 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 169 0.18028012758285952 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 160 0.17067941072933446 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 158 0.16854591809521777 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 155 0.16534567914404275 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 153 0.16321218650992608 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 146 0.15574496229051768 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 144 0.15361146965640102 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 137 0.14614424543699264 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 129 0.1376102749005259 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.1344100359493509 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 112 0.11947558751053411 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 102 0.10880812433995073 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 94 0.10027415380348399 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.333731585291702E-4 0.0 12-13 0.0 0.0 0.0 0.0010667463170583404 0.0 14-15 0.0 0.0 0.0 0.0016001194755875107 0.0 16-17 0.0 0.0 0.0 0.005333731585291702 0.0 18-19 0.0 0.0 0.0 0.011734209487641745 0.0 20-21 0.0 0.0 0.0 0.019201433707050127 0.0 22-23 0.0 0.0 0.0 0.03520262846292523 0.0 24-25 0.0 0.0 0.0 0.06560489849908793 0.0 26-27 0.0 0.0 0.0 0.12267582646170914 0.0 28-29 0.0 0.0 0.0 0.23895117502106822 0.0 30-31 0.0 0.0 0.0 0.4725686184568448 0.0 32-33 0.0 0.0 0.0 0.8331288736225638 0.0 34-35 0.0 0.0 0.0 1.2987636410185295 0.0 36-37 0.0 0.0 0.0 1.9318775801926544 0.0 38-39 0.0 0.0 0.0 2.6503312247314463 0.0 40-41 0.0 0.0 0.0 3.5720000426698526 0.0 42-43 0.0 0.0 0.0 4.284053209306295 0.0 44-45 0.0 0.0 0.0 4.862229713151915 0.0 46-47 0.0 0.0 0.0 5.550814460813074 0.0 48-49 0.0 0.0 0.0 6.319405182253608 0.0 50-51 0.0 0.0 0.0 6.928517329293921 0.0 52-53 0.0 0.0 0.0 7.418687261982228 0.0 54-55 0.0 0.0 0.0 7.8357850719520385 0.0 56-57 0.0 0.0 0.0 8.260883479299789 0.0 58-59 0.0 0.0 0.0 8.603309047075514 0.0 60-61 0.0 0.0 0.0 9.014006379142977 0.0 62-63 0.0 0.0 0.0 9.335097020577535 0.0 64-65 0.0 0.0 0.0 9.672722229926501 0.0 66-67 0.0 0.0 0.0 10.0274153803484 0.0 68-69 0.0 0.0 0.0 10.465848116659377 0.0 70-71 0.0 0.0 0.0 10.969885751469443 0.0 72-73 0.0 0.0 0.0 11.388583680914842 0.0 74-75 0.0 0.0 0.0 11.832350148811113 0.0 76-77 0.0 0.0 0.0 12.260115421951506 0.0 78-79 0.0 0.0 0.0 12.807356282602434 0.0 80-81 0.0 0.0 0.0 13.371665084326295 0.0 82-83 0.0 0.0 0.0 13.880503077563123 0.0 84-85 0.0 0.0 0.0 14.402675399763183 0.0 86-87 0.0 0.0 0.0 14.97658491834057 0.0 88-89 0.0 0.0 0.0 15.579296587478531 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCTTTA 20 0.0020807453 71.14074 7 TGTGAGA 30 1.3048941E-4 63.236214 2 CCTTAGG 25 0.0050374377 56.912594 2 GTGAGAC 40 5.4034556E-4 47.427162 3 GTACCCA 45 9.4261975E-4 42.360916 1 GTGTGAG 45 9.4261975E-4 42.360916 1 ACCCATG 45 9.652436E-4 42.15748 3 AGCAAAT 25 0.001624568 37.94173 46-47 GAGTAAT 85 1.580366E-5 33.639553 1 AGAACAC 30 0.003925822 31.694212 70-71 TCTGTTA 30 0.0039723217 31.618107 32-33 CAAATGT 30 0.0039723217 31.618107 48-49 CTGGGAA 60 0.003962274 31.618107 4 TGTAATA 30 0.0039723217 31.618107 52-53 CTAGCCA 30 0.0039723217 31.618107 16-17 AGTAATG 95 3.4935023E-5 29.953997 2 TACTGGG 65 0.005860862 29.185946 2 TAATGGG 65 0.005860862 29.185946 4 GTAATGG 100 4.956964E-5 28.456297 3 TAAAGCA 35 0.008436892 27.101234 44-45 >>END_MODULE