FastQCFastQC Report
Thu 26 May 2016
SRR936341_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936341_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422213
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49090.20266590923259017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35100.14490880859775748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24620.10164258882270057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75200.038.9734541
GGTATCA45650.035.16051
ATCAACG92550.031.3518813
CAACGCA93150.031.1499355
TCAACGC94500.030.7550264
AACGCAG96850.030.1052846
TATCAAC98500.029.6514842
TATACCG1601.9808795E-929.584185
GTACATG122850.028.8285331
TACATGG120800.028.6057532
ACATGGG120300.027.5823923
ACGCAGA114600.025.3184687
CGCAGAG116850.024.7509578
CATGGGG63200.024.0418284
TACCTGG19350.022.9955792
GTACCTG20650.022.4699521
GCAGAGT131750.021.484749
AGTACAT85250.021.0446782
CATGGGA55000.020.9133264
GAGTACA97700.020.4994351
Produced by FastQC (version 0.11.5)