##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936341_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2422213 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.730893195602533 33.0 31.0 34.0 30.0 34.0 2 31.818101876259437 34.0 31.0 34.0 30.0 34.0 3 31.890520362990372 34.0 31.0 34.0 30.0 34.0 4 35.382470492892246 37.0 35.0 37.0 33.0 37.0 5 35.369143423802946 37.0 35.0 37.0 33.0 37.0 6 35.45891793991693 37.0 35.0 37.0 33.0 37.0 7 35.40269786348269 37.0 35.0 37.0 33.0 37.0 8 35.40241547708645 37.0 35.0 37.0 33.0 37.0 9 37.068709894629414 39.0 37.0 39.0 33.0 39.0 10-11 36.990453151725305 39.0 37.0 39.0 33.0 39.0 12-13 36.89307112958274 39.0 37.0 39.0 33.0 39.0 14-15 38.14219042668832 40.0 38.0 41.0 33.0 41.0 16-17 38.08958956128136 40.0 38.0 41.0 33.0 41.0 18-19 38.03462659972513 40.0 38.0 41.0 33.0 41.0 20-21 37.9608934061538 40.0 38.0 41.0 33.0 41.0 22-23 37.804015584096035 40.0 38.0 41.0 32.5 41.0 24-25 37.95089284055531 40.0 38.0 41.0 33.0 41.0 26-27 37.902867130182194 40.0 38.0 41.0 33.0 41.0 28-29 37.774130929030605 40.0 38.0 41.0 32.5 41.0 30-31 37.62183817030129 40.0 38.0 41.0 32.0 41.0 32-33 37.448791249985035 40.0 37.0 41.0 32.0 41.0 34-35 37.18866961741185 40.0 37.0 41.0 31.5 41.0 36-37 36.98724245142768 40.0 37.0 41.0 31.0 41.0 38-39 36.82468366737359 40.0 36.5 41.0 30.5 41.0 40-41 36.63817385176283 40.0 36.0 41.0 30.0 41.0 42-43 36.4443810267718 40.0 36.0 41.0 30.0 41.0 44-45 36.257711646333334 40.0 36.0 41.0 30.0 41.0 46-47 35.98225548289932 39.0 35.0 41.0 28.5 41.0 48-49 35.809130328340245 39.0 35.0 41.0 28.0 41.0 50-51 35.49037966520698 38.5 35.0 40.5 28.0 41.0 52-53 35.500339152667415 39.0 35.0 40.5 28.0 41.0 54-55 35.569377878824035 39.0 35.0 41.0 28.0 41.0 56-57 35.39863959115074 39.0 35.0 41.0 27.5 41.0 58-59 35.16214263568068 38.5 35.0 41.0 27.0 41.0 60-61 34.86625742657644 38.0 34.5 40.0 26.0 41.0 62-63 34.55854501647873 37.5 34.0 40.0 26.0 41.0 64-65 34.22807407936462 37.0 34.0 40.0 26.0 41.0 66-67 33.87564326506381 36.5 34.0 39.5 26.0 41.0 68-69 33.49581085561014 36.0 34.0 39.0 25.0 41.0 70-71 33.1022467883708 35.0 33.0 39.0 24.0 40.5 72-73 32.713289046008754 35.0 33.0 37.5 23.5 40.0 74-75 32.307589794951966 35.0 33.0 37.0 22.0 39.0 76-77 31.926647656502546 35.0 32.5 36.5 20.5 39.0 78-79 31.551780541182794 35.0 32.0 36.0 20.0 39.0 80-81 31.180596421536833 35.0 32.0 36.0 18.5 37.0 82-83 30.850410760738217 35.0 31.5 35.0 17.5 37.0 84-85 30.55243634643196 35.0 31.0 35.0 14.5 36.5 86-87 30.245627036102935 34.0 31.0 35.0 8.5 36.0 88-89 29.946072661652796 34.0 31.0 35.0 6.0 36.0 90-91 29.6157672756277 34.0 30.5 35.0 2.0 35.5 92-93 29.322809141888015 34.0 30.0 35.0 2.0 35.0 94-95 28.96739427952868 34.0 30.0 35.0 2.0 35.0 96-97 28.562070511552864 34.0 29.0 35.0 2.0 35.0 98-99 28.094774695701823 34.0 29.0 35.0 2.0 35.0 100-101 24.882429414754192 30.5 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15587303967108213 1101 2 0.08408737893263662 1101 3 0.05368077725882614 1101 4 -0.04465621246188789 1101 5 0.2971745579914753 1101 6 0.13133934183814944 1101 7 0.23257992503306468 1101 8 0.18659876025466104 1101 9 0.2089268900287493 1101 10-11 0.3433365916068354 1101 12-13 0.2240559339909538 1101 14-15 0.3894564561838365 1101 16-17 0.325977682973118 1101 18-19 0.4465808674996765 1101 20-21 0.5089279606991042 1101 22-23 0.48263421262966943 1101 24-25 0.45502135752491313 1101 26-27 0.37335786175906094 1101 28-29 0.3583229909165908 1101 30-31 0.3600444256289208 1101 32-33 0.43912502296087297 1101 34-35 0.21819426879982728 1101 36-37 0.10319761260010551 1101 38-39 0.06960396945272151 1101 40-41 0.11666445883719945 1101 42-43 0.1487488023618937 1101 44-45 0.1666079967923082 1101 46-47 0.059920187165396044 1101 48-49 0.07996002194108343 1101 50-51 0.15930946899120357 1101 52-53 0.19094522714760842 1101 54-55 0.13750847953422607 1101 56-57 0.14151074856287948 1101 58-59 0.24976547316983755 1101 60-61 0.08304955747269815 1101 62-63 -0.02067702521357262 1101 64-65 0.0917613550450298 1101 66-67 0.1642485859534375 1101 68-69 0.20049087976232727 1101 70-71 0.23590980519774973 1101 72-73 0.20756188294247124 1101 74-75 0.015604828534542037 1101 76-77 0.09835656339516419 1101 78-79 0.2723953928614975 1101 80-81 0.20492376090246367 1101 82-83 0.4084556822854033 1101 84-85 0.22859058647642883 1101 86-87 0.08315587009193948 1101 88-89 0.058518737215738525 1101 90-91 0.06362954953836919 1101 92-93 0.14239724949295507 1101 94-95 0.27827447345983103 1101 96-97 0.24098332371040243 1101 98-99 0.38716291880311715 1101 100-101 0.4316748387244509 1102 1 0.2531973658687221 1102 2 0.2309869421715014 1102 3 0.2005121716301339 1102 4 0.1522431563355795 1102 5 0.23321637616039936 1102 6 0.17547204969543628 1102 7 0.16769482960694404 1102 8 0.1927620951488649 1102 9 0.18822574348786958 1102 10-11 0.27267407027833457 1102 12-13 0.22253631725553902 1102 14-15 0.4079696794572385 1102 16-17 0.29978711255753865 1102 18-19 0.3209797290338372 1102 20-21 0.244551135094504 1102 22-23 0.39904923018544025 1102 24-25 0.39461653510366546 1102 26-27 0.31102212185119527 1102 28-29 0.31139238567998717 1102 30-31 0.31784412216308056 1102 32-33 0.0804939864989862 1102 34-35 0.13467917088257053 1102 36-37 0.15951699243726836 1102 38-39 0.035668226159288 1102 40-41 0.17796325419266168 1102 42-43 0.2348099388558751 1102 44-45 0.15731484161000253 1102 46-47 0.17158445229013353 1102 48-49 0.11318227967110062 1102 50-51 0.06385864287832277 1102 52-53 0.017382029002902755 1102 54-55 0.06663721964316949 1102 56-57 0.12255111070884084 1102 58-59 0.12122575325238216 1102 60-61 0.07234601587126832 1102 62-63 0.3618910058293352 1102 64-65 0.2096257845528129 1102 66-67 0.32754539251649106 1102 68-69 0.2854943694135841 1102 70-71 0.3927771209021884 1102 72-73 0.15324719394691755 1102 74-75 0.2852237943030147 1102 76-77 0.21073820291133671 1102 78-79 0.30919091165059953 1102 80-81 0.344113493847491 1102 82-83 0.218373428997797 1102 84-85 0.18377458866149254 1102 86-87 0.03140117370303486 1102 88-89 0.14122804709288062 1102 90-91 0.27195704009823984 1102 92-93 0.28839723531017114 1102 94-95 0.20986243977892016 1102 96-97 0.2993570341350029 1102 98-99 0.20222375494231315 1102 100-101 0.15039270585326037 1103 1 0.38794165888420196 1103 2 0.3251828526082612 1103 3 0.38786246615683595 1103 4 0.3279317011515417 1103 5 0.4127906840471809 1103 6 0.321070742234177 1103 7 0.45213121442662185 1103 8 0.3694841374147657 1103 9 0.3536078609934563 1103 10-11 0.43193961801414105 1103 12-13 0.4380575577022725 1103 14-15 0.3923229205891019 1103 16-17 0.63480342906022 1103 18-19 0.5025921500941735 1103 20-21 0.5178745230035204 1103 22-23 0.6322031548440563 1103 24-25 0.635066385275536 1103 26-27 0.6739313837190224 1103 28-29 0.5439188297440012 1103 30-31 0.537165261088866 1103 32-33 0.547337318633943 1103 34-35 0.5792416450674338 1103 36-37 0.6198822314173853 1103 38-39 0.5809358159112037 1103 40-41 0.6570184269879107 1103 42-43 0.5005350909811597 1103 44-45 0.4741906461723744 1103 46-47 0.5554363111135814 1103 48-49 0.7081845366832269 1103 50-51 0.6788983502339505 1103 52-53 0.7181245235454838 1103 54-55 0.7599865498101437 1103 56-57 0.6822268904708508 1103 58-59 0.7264152307585405 1103 60-61 0.7613966523397693 1103 62-63 0.663397271491263 1103 64-65 0.6942880267025657 1103 66-67 0.7253888308113048 1103 68-69 0.7758435238369259 1103 70-71 0.8132313825068422 1103 72-73 0.7075077057801806 1103 74-75 0.821375463733709 1103 76-77 0.7412917239840624 1103 78-79 0.740342786264204 1103 80-81 0.6932565429192188 1103 82-83 0.5810558745284204 1103 84-85 0.7144622592197969 1103 86-87 0.8386251290766431 1103 88-89 0.6317898557714052 1103 90-91 0.7393286067412035 1103 92-93 0.7064780657835819 1103 94-95 0.6906584335377914 1103 96-97 0.8085491174642456 1103 98-99 0.8459888481533504 1103 100-101 0.8944587934877788 1104 1 0.3861750732329341 1104 2 0.2543835353533659 1104 3 0.28824728927322596 1104 4 0.32757855713110473 1104 5 0.30057744045397783 1104 6 0.24451292701851912 1104 7 0.3520079602390922 1104 8 0.3305292359834908 1104 9 0.29786303870798747 1104 10-11 0.361824950073391 1104 12-13 0.35794666355545957 1104 14-15 0.5048187659535657 1104 16-17 0.4448353158879499 1104 18-19 0.39149676312449344 1104 20-21 0.47075706107037263 1104 22-23 0.5577044808652332 1104 24-25 0.5079449245056793 1104 26-27 0.4301399671960411 1104 28-29 0.509929150551109 1104 30-31 0.434689444529873 1104 32-33 0.4191490686356687 1104 34-35 0.35016715937668863 1104 36-37 0.2381746589016842 1104 38-39 0.18270664275291892 1104 40-41 0.26950042356725845 1104 42-43 0.32992022223302087 1104 44-45 0.28954955474961963 1104 46-47 0.3394744610543725 1104 48-49 0.41170022389456307 1104 50-51 0.3712363367590825 1104 52-53 0.38511360724061205 1104 54-55 0.49677048106477173 1104 56-57 0.5708406790137488 1104 58-59 0.5626542123188827 1104 60-61 0.4124674230939078 1104 62-63 0.5181529442998709 1104 64-65 0.5660902556645269 1104 66-67 0.6328893328297553 1104 68-69 0.5540959110212569 1104 70-71 0.5358969101109281 1104 72-73 0.6459225911468565 1104 74-75 0.6014935419377991 1104 76-77 0.7022169440066364 1104 78-79 0.6090796731958044 1104 80-81 0.6274414899822354 1104 82-83 0.7232049982465476 1104 84-85 0.595972053773071 1104 86-87 0.6431101446911462 1104 88-89 0.6888667580751502 1104 90-91 0.8302926206730845 1104 92-93 0.7601100827898897 1104 94-95 0.6477163803169113 1104 96-97 0.6773669332338557 1104 98-99 0.6366787426948477 1104 100-101 0.7453484913765323 1105 1 0.3409936673372371 1105 2 0.32480478077461683 1105 3 0.29374615548637806 1105 4 0.23370972754798913 1105 5 0.3058975172026237 1105 6 0.29800150193566566 1105 7 0.39466451003850267 1105 8 0.24640225185650877 1105 9 0.4257803594824523 1105 10-11 0.33624061377476977 1105 12-13 0.3408744500546774 1105 14-15 0.3976650070140906 1105 16-17 0.4508182218708612 1105 18-19 0.3295810994231232 1105 20-21 0.3071668814087687 1105 22-23 0.4797871600907655 1105 24-25 0.4585467036788913 1105 26-27 0.38051934971827706 1105 28-29 0.4723550183341203 1105 30-31 0.5079146320407801 1105 32-33 0.5186977342557597 1105 34-35 0.22414239049478368 1105 36-37 0.29323963360951666 1105 38-39 0.3906017238622823 1105 40-41 0.40592332606158976 1105 42-43 0.4607962906090961 1105 44-45 0.47216118914697347 1105 46-47 0.4430393485478348 1105 48-49 0.5607059800431742 1105 50-51 0.4676431045944227 1105 52-53 0.5687325676452915 1105 54-55 0.6249232806461436 1105 56-57 0.7427990777578657 1105 58-59 0.6713451245812223 1105 60-61 0.5293106988657499 1105 62-63 0.3359795621264823 1105 64-65 0.42422953701809263 1105 66-67 0.43367426218611627 1105 68-69 0.5324667465349506 1105 70-71 0.5847522214662462 1105 72-73 0.5930641400741195 1105 74-75 0.6116431145873733 1105 76-77 0.6122487772527592 1105 78-79 0.45895757307370033 1105 80-81 0.5830166687717018 1105 82-83 0.6127043877459322 1105 84-85 0.5235448777744622 1105 86-87 0.6286216569883756 1105 88-89 0.5538545480629402 1105 90-91 0.49925738606642156 1105 92-93 0.5247272116927313 1105 94-95 0.5199407828984555 1105 96-97 0.6396837830507067 1105 98-99 0.666578010094117 1105 100-101 0.5561870575722416 1106 1 0.24980713083050432 1106 2 0.29323979403721623 1106 3 0.2793581898668869 1106 4 0.23060369291485472 1106 5 0.3113138305007155 1106 6 0.3295514517122129 1106 7 0.23179196933345025 1106 8 0.25259567529131033 1106 9 0.1620477046167892 1106 10-11 0.2987802361665999 1106 12-13 0.33863695028022534 1106 14-15 0.2905251572634029 1106 16-17 0.3789328275716741 1106 18-19 0.2769568490156189 1106 20-21 0.3200923522160082 1106 22-23 0.35387095806618873 1106 24-25 0.2139888805496355 1106 26-27 0.29348782623355163 1106 28-29 0.2930145995848292 1106 30-31 0.3680290933867667 1106 32-33 0.35470704514191453 1106 34-35 0.2169411899438245 1106 36-37 0.3482147624762675 1106 38-39 0.37925363024817926 1106 40-41 0.2399516677253999 1106 42-43 0.45699645060236094 1106 44-45 0.44536141530285533 1106 46-47 0.45393543111879353 1106 48-49 0.3947114655264983 1106 50-51 0.4541275834434302 1106 52-53 0.3956210595584082 1106 54-55 0.42167890597320223 1106 56-57 0.40877645013917174 1106 58-59 0.5617542502120259 1106 60-61 0.6581176062440903 1106 62-63 0.5354574788457853 1106 64-65 0.41600973273917674 1106 66-67 0.376447498801717 1106 68-69 0.43340625584884407 1106 70-71 0.5456780772196836 1106 72-73 0.6067426618807232 1106 74-75 0.4754593749553564 1106 76-77 0.4092161229885747 1106 78-79 0.44301298126704225 1106 80-81 0.6893099376265432 1106 82-83 0.5498122989917782 1106 84-85 0.6720267462070701 1106 86-87 0.6365025281697356 1106 88-89 0.388042582250975 1106 90-91 0.5559936437879003 1106 92-93 0.5539082386570122 1106 94-95 0.6313039134734062 1106 96-97 0.48696179239768256 1106 98-99 0.482493129457513 1106 100-101 0.6631440578789949 1107 1 0.05967389277945756 1107 2 0.17064201183167782 1107 3 0.2693878745999676 1107 4 0.1461031102206718 1107 5 0.12305025918498558 1107 6 0.2305625026377882 1107 7 0.21033898063746648 1107 8 0.29972608216817775 1107 9 0.3304111734544364 1107 10-11 0.3108542883683967 1107 12-13 0.19333208418409953 1107 14-15 0.3757062390978163 1107 16-17 0.1672651311615425 1107 18-19 0.2876860314489349 1107 20-21 0.3025970522298209 1107 22-23 0.4397850600926745 1107 24-25 0.47053705641492627 1107 26-27 0.3845481642375361 1107 28-29 0.4057183667916817 1107 30-31 0.4242384050797696 1107 32-33 0.4824320399518527 1107 34-35 0.23918654348494073 1107 36-37 0.3157340070994792 1107 38-39 0.3358805463360852 1107 40-41 0.45580326556467554 1107 42-43 0.09289942809108709 1107 44-45 0.26720339062375587 1107 46-47 0.50361124802793 1107 48-49 0.38626136590192317 1107 50-51 0.30137713453318327 1107 52-53 0.43332352407774977 1107 54-55 0.5065473428060869 1107 56-57 0.4245927306727211 1107 58-59 0.6332248562057075 1107 60-61 0.4663136197721869 1107 62-63 0.5204881519671574 1107 64-65 0.37997801664069186 1107 66-67 0.37090404652267495 1107 68-69 0.39993194277871424 1107 70-71 0.41913459930044183 1107 72-73 0.1972930907750694 1107 74-75 0.3178958058728014 1107 76-77 0.3252730078367918 1107 78-79 0.42455886192101033 1107 80-81 0.5109443100495987 1107 82-83 0.6017870491859405 1107 84-85 0.49414766207496186 1107 86-87 0.36284303496893955 1107 88-89 0.42076547208331405 1107 90-91 0.7248403476576684 1107 92-93 0.49084162128569275 1107 94-95 0.6586584901247825 1107 96-97 0.8848639692505387 1107 98-99 1.0354118111542974 1107 100-101 0.8530068331906264 1108 1 -0.005273490925556956 1108 2 0.06882513700871229 1108 3 0.0976650767444518 1108 4 -0.010170419930851438 1108 5 0.08322524218574756 1108 6 0.07782528895683782 1108 7 0.05074455019705937 1108 8 0.10715591719032602 1108 9 0.1044392021543814 1108 10-11 0.10785786118263019 1108 12-13 0.0920212191948977 1108 14-15 -0.006655499814591792 1108 16-17 0.08579338241853662 1108 18-19 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5 3079.0 6 3017.0 7 3162.0 8 3755.0 9 4593.0 10 5344.0 11 6543.0 12 8390.0 13 12020.0 14 12634.0 15 10579.0 16 12504.0 17 15383.0 18 14342.0 19 10493.0 20 10349.0 21 11062.0 22 11644.0 23 12807.0 24 14510.0 25 17319.0 26 20324.0 27 24758.0 28 29245.0 29 36209.0 30 44074.0 31 55182.0 32 70438.0 33 93199.0 34 130774.0 35 198978.0 36 332240.0 37 537944.0 38 521454.0 39 95769.0 40 65.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.85636180623907 17.588762449418592 12.543817946988492 24.011057797353843 2 16.16900077872552 18.959943119179687 39.76355812323021 25.10749797886458 3 20.415645801799034 23.24640395447335 27.748159965911988 28.589790277815624 4 12.919472785464443 14.522263435515143 35.41114113112323 37.147122647897184 5 14.38977150881145 37.440086143007136 33.15353843219231 15.0166039159891 6 35.05416405528127 33.87897869852681 16.59862614291978 14.468231103272139 7 31.28637091969631 31.352759782551704 19.01669341350642 18.344175884245566 8 28.05249295715913 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27.329464655857272 23.591300678326103 32-33 23.24486103403626 25.677281525817875 27.400214213043704 23.67764322710216 34-35 23.82803250702481 25.784692291006756 27.12460115476768 23.262674047200758 36-37 23.846712402097356 25.56729763917819 27.0588877027663 23.52710225595816 38-39 23.585378565602184 25.75293036984558 27.164746754282394 23.496944310269843 40-41 23.69126032653251 25.653094059816723 27.310214181162813 23.34543143248796 42-43 23.671288158838504 25.6471658530899 27.00328846250377 23.67825752556783 44-45 23.483104296835617 25.84144514347334 27.0936394916705 23.581811068020542 46-47 23.813962616923252 26.07551967389496 26.638087385960535 23.47243032322125 48-49 23.980301617221873 26.040975706918605 26.541236616085907 23.437486059773615 50-51 23.78026663523662 26.05557639824966 26.582908087039094 23.581248879474632 52-53 24.069246303158916 25.973878581818916 26.487372822048073 23.4695022929741 54-55 23.803163902566936 26.016663430404602 26.46837093206007 23.711801734968393 56-57 23.67047560094229 26.34537306175419 26.293097368043288 23.69105396926023 58-59 23.83508414626633 25.92917271781166 26.491931795151462 23.743811340770545 60-61 23.696756393132564 26.077172905866764 26.696712624799385 23.52935807620129 62-63 23.58033974314431 26.124316660772312 26.75750545271256 23.537838143370816 64-65 23.58547735102003 26.078530017976455 26.577555472340848 23.75843715866267 66-67 23.611179957527924 26.27056897605548 26.354256260628127 23.763994805788464 68-69 23.56304014037711 26.067293630770273 26.705675061756263 23.663991167096356 70-71 23.671398483589503 25.91960958465685 26.58798097206418 23.821010959689467 72-73 23.589058935619526 26.239959707104838 26.32990513205445 23.841076225221187 74-75 23.47034295836392 26.443723467334664 26.31332933056874 23.77260424373268 76-77 23.703754718758077 26.334476608360607 26.202004956173997 23.75976371670732 78-79 23.66597595888861 26.316464201263766 26.393798026369424 23.623761813478197 80-81 23.636974278102844 26.49458714564816 26.23302013333715 23.635418442911842 82-83 23.86165029460582 26.474984280705627 26.1587521151812 23.50461330950736 84-85 23.72294950629828 26.51955535105949 25.98876221288898 23.76873292975325 86-87 23.62233849116874 26.184743154816175 26.343567061299268 23.84935129271582 88-89 23.709017498869233 26.377937399112817 26.178341570292176 23.734703531725778 90-91 23.57000371896573 26.533824325356374 26.20326804113442 23.692903914543482 92-93 23.60190615911843 26.329211898865246 26.2535548539514 23.815327088064926 94-95 23.704391652909603 26.154504233330854 26.319817182838324 23.821286930921218 96-97 23.767328472875345 26.17598231404393 26.257195029765207 23.799494183315513 98-99 23.465799645109538 26.112754967357088 26.211006166517326 24.210439221016053 100-101 23.546234548596274 26.22651025779922 26.09577319442244 24.131481999182064 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 153.0 1 124.0 2 183.0 3 294.0 4 241.5 5 222.5 6 454.5 7 878.0 8 1362.0 9 1577.5 10 1450.5 11 1410.5 12 1560.0 13 1790.5 14 2046.5 15 2274.0 16 2459.5 17 2591.0 18 2745.0 19 2889.5 20 2933.5 21 3107.5 22 3452.5 23 3921.0 24 4575.5 25 5588.5 26 7424.5 27 9729.5 28 12106.5 29 15037.5 30 18489.5 31 23079.5 32 28300.5 33 32214.0 34 35431.5 35 39887.0 36 46851.5 37 53175.5 38 57503.5 39 60851.5 40 63186.5 41 66177.5 42 68709.5 43 72495.0 44 77404.5 45 81157.0 46 83521.5 47 85243.5 48 88306.0 49 90851.0 50 90474.0 51 89996.0 52 91538.0 53 93048.0 54 94601.5 55 91975.5 56 85589.0 57 79307.0 58 75366.5 59 69396.0 60 59023.5 61 50101.0 62 43048.0 63 34740.0 64 26267.0 65 20229.5 66 14748.5 67 11321.0 68 8602.5 69 6281.5 70 4962.0 71 3435.0 72 2638.0 73 2281.0 74 1766.5 75 1369.0 76 972.0 77 532.5 78 305.5 79 180.0 80 138.0 81 109.5 82 84.5 83 57.0 84 44.0 85 38.0 86 30.0 87 24.0 88 24.0 89 24.0 90 22.5 91 16.5 92 20.5 93 21.5 94 17.0 95 20.5 96 24.0 97 18.5 98 14.5 99 14.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046073569913133154 2 0.012674360182197024 3 0.010197286530953305 4 0.005160570106757746 5 0.008009204805688022 6 0.0052431392284658694 7 0.004954147302487436 8 0.010321140213515491 9 0.015399141198565114 10-11 0.002250008566546377 12-13 0.009309668472590973 14-15 0.009123887948747694 16-17 0.01948631272311725 18-19 0.012240872293229375 20-21 0.003756895037719639 22-23 0.009784440922412686 24-25 0.021777605850517686 26-27 0.007204155869033813 28-29 0.01833034501920351 30-31 0.015234002955148866 32-33 0.013417482277570138 34-35 0.2678955153820081 36-37 0.4732655633505394 38-39 0.4699215139213604 40-41 0.4919881116978565 42-43 0.48154311780177883 44-45 0.4972312509263223 46-47 0.5033413659327235 48-49 0.5107106600451735 50-51 0.5213827190259486 52-53 0.5099262533889464 54-55 0.4964468442700952 56-57 0.4919055425761484 58-59 0.4894903957661857 60-61 0.4869307529932339 62-63 0.483669272685763 64-65 0.5001830970273877 66-67 0.47281143318114466 68-69 0.5009262191227609 70-71 0.48104770307153005 72-73 0.48973810313131005 74-75 0.5041670571498047 76-77 0.4907082903113805 78-79 0.49060507890924543 80-81 0.49260738011066735 82-83 0.4937014209733 84-85 0.4934330713277486 86-87 0.49527023428575445 88-89 0.5095753346216868 90-91 0.5342635020124159 92-93 0.5224767598885812 94-95 0.49277251835408364 96-97 0.46488479749716477 98-99 0.4560499014743955 100-101 0.46515314714271616 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2422213.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.5993039295385 #Duplication Level Percentage of deduplicated Percentage of total 1 69.55965127769565 31.023120285666593 2 14.612150276302144 13.033834624737786 3 5.934531803379035 7.940279627352415 4 2.983556402192312 5.3225815508918135 5 1.8238860700901478 4.067202458640104 6 1.1136969274641833 2.9802064652020985 7 0.7765298541811617 2.424288368389009 8 0.536095914303554 1.9127603693926423 9 0.3890772085131346 1.5617315407080343 >10 2.031876894078776 16.282945538583476 >50 0.1524258269800748 4.707287129914418 >100 0.08178121633285043 6.659952852234362 >500 0.0035320094610154745 1.1061582792285032 >1k 0.0012083190261367239 0.97765090905871 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4909 0.20266590923259017 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3510 0.14490880859775748 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2462 0.10164258882270057 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 8.256912170812394E-5 0.0 0.0 0.0 5 0.0 8.256912170812394E-5 0.0 0.0 0.0 6 0.0 8.256912170812394E-5 0.0 0.0 0.0 7 4.128456085406197E-5 8.256912170812394E-5 0.0 0.0 0.0 8 8.256912170812394E-5 8.256912170812394E-5 0.0 0.0 0.0 9 8.256912170812394E-5 8.256912170812394E-5 0.0 0.0 0.0 10-11 8.256912170812394E-5 8.256912170812394E-5 0.0 0.0 0.0 12-13 1.238536825621859E-4 8.256912170812394E-5 0.0 0.0 0.0 14-15 1.238536825621859E-4 1.6513824341624788E-4 0.0 0.0 0.0 16-17 1.238536825621859E-4 1.6513824341624788E-4 0.0 8.256912170812394E-5 0.0 18-19 1.444959629892169E-4 1.6513824341624788E-4 0.0 8.256912170812394E-5 0.0 20-21 1.6513824341624788E-4 1.6513824341624788E-4 0.0 1.6513824341624788E-4 0.0 22-23 3.3027648683249576E-4 1.6513824341624788E-4 0.0 3.509187672595267E-4 0.0 24-25 3.715610476865577E-4 1.6513824341624788E-4 0.0 6.811952540920225E-4 0.0 26-27 3.715610476865577E-4 1.6513824341624788E-4 0.0 8.463334975082704E-4 0.0 28-29 4.541301693946816E-4 1.6513824341624788E-4 0.0 0.0016100978733084167 0.0 30-31 4.954147302487436E-4 1.6513824341624788E-4 0.0 0.003653683635584484 0.0 32-33 5.160570106757746E-4 1.6513824341624788E-4 0.0 0.006873879382201317 0.0 34-35 5.986261323838985E-4 1.6513824341624788E-4 0.0 0.011373896515294073 0.0 36-37 6.192684128109295E-4 1.6513824341624788E-4 0.0 0.018908328871160382 0.0 38-39 6.192684128109295E-4 1.6513824341624788E-4 0.0 0.03201617694232506 0.0 40-41 6.192684128109295E-4 1.6513824341624788E-4 0.0 0.04914926969676077 0.0 42-43 7.224798149460844E-4 1.6513824341624788E-4 0.0 0.06539474439283416 0.0 44-45 8.256912170812393E-4 1.6513824341624788E-4 0.0 0.08444756922698377 0.0 46-47 9.289026192163942E-4 1.6513824341624788E-4 0.0 0.10802105347465313 0.0 48-49 9.908294604974873E-4 1.6513824341624788E-4 0.0 0.13413353821484733 0.0 50-51 9.908294604974873E-4 2.477073651243718E-4 0.0 0.1647460400881343 0.0 52-53 9.908294604974873E-4 2.477073651243718E-4 0.0 0.19589524125252405 2.0642280427030985E-5 54-55 9.908294604974873E-4 2.477073651243718E-4 0.0 0.22764306854929767 4.128456085406197E-5 56-57 9.908294604974873E-4 2.477073651243718E-4 0.0 0.26605835242400233 6.192684128109296E-5 58-59 9.908294604974873E-4 2.477073651243718E-4 0.0 0.3110791660353569 8.256912170812394E-5 60-61 9.908294604974873E-4 2.477073651243718E-4 0.0 0.36270550938336144 8.256912170812394E-5 62-63 9.908294604974873E-4 2.889919259784338E-4 0.0 0.4108845919000517 1.238536825621859E-4 64-65 0.0010733985822056111 2.889919259784338E-4 0.0 0.463026992258732 1.238536825621859E-4 66-67 0.001114683143059673 3.096342064054648E-4 0.0 0.5272657689476523 1.238536825621859E-4 68-69 0.001114683143059673 3.3027648683249576E-4 0.0 0.6050871661575592 1.238536825621859E-4 70-71 0.001155967703913735 3.3027648683249576E-4 0.0 0.6898030850300944 1.444959629892169E-4 72-73 0.001217894545194828 3.715610476865577E-4 0.0 0.7745190039026295 1.6513824341624788E-4 74-75 0.001279821386475921 3.715610476865577E-4 0.0 0.8619184192306787 1.6513824341624788E-4 76-77 0.001279821386475921 3.715610476865577E-4 0.0 0.9615380645715302 1.6513824341624788E-4 78-79 0.001300463666902952 3.715610476865577E-4 0.0 1.076783916195644 1.6513824341624788E-4 80-81 0.0016100978733084167 3.715610476865577E-4 0.0 1.2093279988176102 1.6513824341624788E-4 82-83 0.0016513824341624786 4.3348788896765066E-4 0.0 1.3520280834096754 1.6513824341624788E-4 84-85 0.0017339515558706027 4.541301693946816E-4 0.0 1.4885148415932044 1.6513824341624788E-4 86-87 0.0018165206775787265 4.541301693946816E-4 0.0 1.6353640245511025 1.6513824341624788E-4 88-89 0.0018165206775787265 4.541301693946816E-4 0.0 1.808201838566633 1.6513824341624788E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7520 0.0 38.973454 1 GGTATCA 4565 0.0 35.1605 1 ATCAACG 9255 0.0 31.351881 3 CAACGCA 9315 0.0 31.149935 5 TCAACGC 9450 0.0 30.755026 4 AACGCAG 9685 0.0 30.105284 6 TATCAAC 9850 0.0 29.651484 2 TATACCG 160 1.9808795E-9 29.58418 5 GTACATG 12285 0.0 28.828533 1 TACATGG 12080 0.0 28.605753 2 ACATGGG 12030 0.0 27.582392 3 ACGCAGA 11460 0.0 25.318468 7 CGCAGAG 11685 0.0 24.750957 8 CATGGGG 6320 0.0 24.041828 4 TACCTGG 1935 0.0 22.995579 2 GTACCTG 2065 0.0 22.469952 1 GCAGAGT 13175 0.0 21.48474 9 AGTACAT 8525 0.0 21.044678 2 CATGGGA 5500 0.0 20.913326 4 GAGTACA 9770 0.0 20.499435 1 >>END_MODULE