FastQCFastQC Report
Thu 26 May 2016
SRR936341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936341_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422213
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57350.23676695649804536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43870.18111536846676984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27050.11167473711023762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48950.038.971991
GTATCAA81100.037.1316071
ATCAACG97250.030.339873
TCAACGC98800.029.863894
CAACGCA99150.029.7106295
TATCAAC102400.029.0528092
AACGCAG102500.028.8321556
GTACATG123750.027.98641
TACATGG121650.027.965822
ACATGGG121350.027.128933
TACCTGG20500.024.071332
ACGCAGA121550.024.0012077
CATGGGG63100.023.3047944
CGCAGAG125800.023.1526498
GTACCTG22600.022.7340181
CATGGGA55050.021.9733284
ACCCCGC3550.021.3798478
AGTACAT87250.020.6651572
GCAGAGT140800.020.3155069
GAGTACA98100.020.2706721
Produced by FastQC (version 0.11.5)