FastQCFastQC Report
Thu 26 May 2016
SRR936340_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936340_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226388
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63110.2834636190996358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41990.18860144772609266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29360.1318727912654937No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG24200.10869623803218485No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG23470.10541738457088341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75700.036.5219351
GGTATCA46350.035.2375871
ATCAACG90250.030.2034113
TCAACGC90700.030.0535564
GTACATG97100.029.2528931
CAACGCA93650.029.1068615
AACGCAG95650.028.495056
TATCAAC96650.028.3986072
TACATGG98650.028.2545992
ACATGGG99550.026.9064163
GTACCTG18100.024.847451
ACGCAGA110150.024.529217
CGCAGAG111950.024.1353558
CATGGGG46200.022.5351984
GCAGAGT121050.021.7346029
CATGGGA54400.021.4871444
TACCTGG19600.021.2469942
GATGTGC14950.020.8902878
GGACCGT2309.402174E-820.5733056
AGTACAT68800.020.5661812
Produced by FastQC (version 0.11.5)