##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936340_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2226388 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.619077177922268 33.0 31.0 34.0 30.0 34.0 2 31.710476790209075 33.0 31.0 34.0 30.0 34.0 3 31.774665062873137 34.0 31.0 34.0 30.0 34.0 4 35.27153847397668 37.0 35.0 37.0 33.0 37.0 5 35.25500766263562 37.0 35.0 37.0 33.0 37.0 6 35.34960752573226 37.0 35.0 37.0 33.0 37.0 7 35.28941002197281 37.0 35.0 37.0 33.0 37.0 8 35.29074806367983 37.0 35.0 37.0 33.0 37.0 9 36.94322238531649 39.0 37.0 39.0 33.0 39.0 10-11 36.85516316113814 39.0 37.0 39.0 33.0 39.0 12-13 36.748320598206604 39.0 37.0 39.0 33.0 39.0 14-15 37.96279691590145 40.0 38.0 41.0 33.0 41.0 16-17 37.915880116134296 40.0 38.0 41.0 33.0 41.0 18-19 37.862654218402184 40.0 38.0 41.0 33.0 41.0 20-21 37.78995664726903 40.0 38.0 41.0 32.5 41.0 22-23 37.625690805016916 40.0 38.0 41.0 32.0 41.0 24-25 37.77858194528537 40.0 38.0 41.0 32.5 41.0 26-27 37.73691557805738 40.0 38.0 41.0 32.0 41.0 28-29 37.5949457147631 40.0 38.0 41.0 32.0 41.0 30-31 37.433125537866715 40.0 37.5 41.0 32.0 41.0 32-33 37.24980124758129 40.0 37.0 41.0 31.0 41.0 34-35 36.97685398951126 40.0 37.0 41.0 30.5 41.0 36-37 36.77175519271574 40.0 36.0 41.0 30.0 41.0 38-39 36.61161509134976 40.0 36.0 41.0 30.0 41.0 40-41 36.41870846411317 40.0 36.0 41.0 30.0 41.0 42-43 36.221705964998016 40.0 35.5 41.0 29.5 41.0 44-45 36.03462581544636 39.5 35.0 41.0 29.0 41.0 46-47 35.734663275224264 39.0 35.0 41.0 27.5 41.0 48-49 35.549682041045855 39.0 35.0 41.0 27.0 41.0 50-51 35.23427205859895 38.5 35.0 40.5 26.5 41.0 52-53 35.24764371708795 39.0 35.0 40.5 27.0 41.0 54-55 35.32088140072619 39.0 35.0 41.0 26.5 41.0 56-57 35.16817239403015 39.0 35.0 41.0 26.0 41.0 58-59 34.94119847933065 39.0 35.0 41.0 26.0 41.0 60-61 34.65194498892376 38.0 34.0 40.0 26.0 41.0 62-63 34.350523583490386 38.0 34.0 40.0 25.0 41.0 64-65 34.019171411272424 37.0 34.0 40.0 24.0 41.0 66-67 33.66300954730263 36.5 34.0 39.5 23.0 41.0 68-69 33.28575836736454 36.0 33.0 39.0 22.0 41.0 70-71 32.87869769330413 35.5 33.0 39.0 21.5 40.5 72-73 32.471978154751106 35.0 33.0 38.0 20.0 40.0 74-75 32.05088443703434 35.0 32.0 37.0 19.0 39.5 76-77 31.648160383545008 35.0 32.0 37.0 18.0 39.0 78-79 31.255666577433942 35.0 32.0 36.0 15.0 39.0 80-81 30.858682987870935 35.0 31.0 36.0 10.0 37.5 82-83 30.514761802524987 35.0 31.0 35.0 7.0 37.0 84-85 30.203047941329185 34.5 31.0 35.0 4.5 37.0 86-87 29.872288882261316 34.0 31.0 35.0 2.0 36.0 88-89 29.549116775692287 34.0 30.0 35.0 2.0 36.0 90-91 29.20283054885312 34.0 30.0 35.0 2.0 35.5 92-93 28.88962772885948 34.0 29.5 35.0 2.0 35.0 94-95 28.519694904931214 34.0 29.0 35.0 2.0 35.0 96-97 28.094607723361783 34.0 29.0 35.0 2.0 35.0 98-99 27.58716090816156 34.0 29.0 35.0 2.0 35.0 100-101 24.310250504404443 30.0 14.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18535842936196545 1101 2 0.14596950827933242 1101 3 0.14503904260992684 1101 4 0.14227917852208805 1101 5 0.2047310821057522 1101 6 0.23192422456568806 1101 7 0.36125693635033684 1101 8 0.3694976777074075 1101 9 0.2827078502735745 1101 10-11 0.4145242992435172 1101 12-13 0.3242920230921982 1101 14-15 0.4426512953255042 1101 16-17 0.3910985636922746 1101 18-19 0.4629098926432178 1101 20-21 0.5922778761828553 1101 22-23 0.5526791050246018 1101 24-25 0.41280326847311244 1101 26-27 0.4179271438182468 1101 28-29 0.49119223249879695 1101 30-31 0.4711966313366034 1101 32-33 0.46785799594020716 1101 34-35 0.3725089181662611 1101 36-37 0.39530758638175456 1101 38-39 0.5318012030946164 1101 40-41 0.5089974953446088 1101 42-43 0.5143504305010964 1101 44-45 0.4496927099371746 1101 46-47 0.2556362416736775 1101 48-49 0.2728114001815882 1101 50-51 0.5242560293525358 1101 52-53 0.5449312417824288 1101 54-55 0.6069947427989106 1101 56-57 0.6482215686839865 1101 58-59 0.571750945423581 1101 60-61 0.3308786186366319 1101 62-63 0.3725174067144863 1101 64-65 0.35336430844454725 1101 66-67 0.47856580609944643 1101 68-69 0.4038075387447648 1101 70-71 0.5329366498172945 1101 72-73 0.6072534588087422 1101 74-75 0.28782021354277987 1101 76-77 0.39460288699304513 1101 78-79 0.550416410544539 1101 80-81 0.5132341997811025 1101 82-83 0.6082505766360491 1101 84-85 0.568818631697706 1101 86-87 0.6009613431012077 1101 88-89 0.6479447636966604 1101 90-91 0.4891489702135665 1101 92-93 0.5813040810221999 1101 94-95 0.8806989460292662 1101 96-97 0.8354871602405538 1101 98-99 0.9142231557929605 1101 100-101 1.2141363455165042 1102 1 0.2786031933855426 1102 2 0.2395275846001894 1102 3 0.13906355896557088 1102 4 0.2250151138242913 1102 5 0.2954454712847294 1102 6 0.22293553086838358 1102 7 0.17666452620145634 1102 8 0.21572580620100013 1102 9 0.3534182706532505 1102 10-11 0.27688867428231134 1102 12-13 0.32553665022403067 1102 14-15 0.4816290960482732 1102 16-17 0.43413669016432266 1102 18-19 0.4978564173174149 1102 20-21 0.44759803041421975 1102 22-23 0.3991811496460045 1102 24-25 0.5480897144398824 1102 26-27 0.44775800293065515 1102 28-29 0.46069031203502675 1102 30-31 0.35591654181577326 1102 32-33 0.2507079015751046 1102 34-35 0.17888417344548202 1102 36-37 0.1580850773471667 1102 38-39 0.22892928866363604 1102 40-41 0.0392814767294567 1102 42-43 0.08610916040042582 1102 44-45 0.051141427797468 1102 46-47 0.2471074352476137 1102 48-49 0.3878101453406302 1102 50-51 0.1896524237938806 1102 52-53 0.17697738597188106 1102 54-55 0.2481813288939989 1102 56-57 0.2313732197946834 1102 58-59 0.259597717935506 1102 60-61 0.1773810611991351 1102 62-63 0.3967224397674798 1102 64-65 0.2736382670194146 1102 66-67 0.3040371938686448 1102 68-69 0.47777457648708577 1102 70-71 0.5033805027149185 1102 72-73 0.4001221950167775 1102 74-75 0.43535329703984615 1102 76-77 0.2523893475509773 1102 78-79 0.313932082261168 1102 80-81 0.28029711851462835 1102 82-83 0.57817206550882 1102 84-85 0.5640007587300602 1102 86-87 0.2627043728974492 1102 88-89 0.2402907114093189 1102 90-91 0.38443952293254213 1102 92-93 0.25016733837708216 1102 94-95 0.31248520814724046 1102 96-97 0.3308589725333242 1102 98-99 0.4721399022689283 1102 100-101 0.6109008720912747 1103 1 0.35149626851613647 1103 2 0.26823601178034195 1103 3 0.31317175670680797 1103 4 0.28192172886287636 1103 5 0.2421949222256714 1103 6 0.15479975579538063 1103 7 0.2531155144038948 1103 8 0.2986940955412294 1103 9 0.3109097523830471 1103 10-11 0.4002502607656666 1103 12-13 0.41447370097323954 1103 14-15 0.4917352102473629 1103 16-17 0.5102716675502137 1103 18-19 0.3604310511785158 1103 20-21 0.5306882318977273 1103 22-23 0.6096273454937702 1103 24-25 0.5945017582690468 1103 26-27 0.5535532050711112 1103 28-29 0.4756129296328524 1103 30-31 0.4245921578810652 1103 32-33 0.46835704076372764 1103 34-35 0.4752354559508447 1103 36-37 0.47314318750380835 1103 38-39 0.5457910234150063 1103 40-41 0.49999577542693174 1103 42-43 0.4573342333269963 1103 44-45 0.5238663446661107 1103 46-47 0.46721468158077073 1103 48-49 0.4472304355720311 1103 50-51 0.44055371676368793 1103 52-53 0.48403603415187035 1103 54-55 0.6443010172766961 1103 56-57 0.631442653888648 1103 58-59 0.7448514120422232 1103 60-61 0.7142561738282964 1103 62-63 0.5758931723361158 1103 64-65 0.6672338936582136 1103 66-67 0.6872810643753624 1103 68-69 0.7143445062791969 1103 70-71 0.6524031022920767 1103 72-73 0.6477479592721807 1103 74-75 0.7720532145186993 1103 76-77 0.6102057725312555 1103 78-79 0.6404822620263992 1103 80-81 0.6157880414479777 1103 82-83 0.7719765397234291 1103 84-85 0.8648000817346606 1103 86-87 0.8927799558296421 1103 88-89 0.7244771873927576 1103 90-91 0.9530694381730918 1103 92-93 0.9384727829552233 1103 94-95 0.8697649600333079 1103 96-97 0.9780523706337263 1103 98-99 0.9238043990119671 1103 100-101 1.221202668912678 1104 1 0.3285313941798087 1104 2 0.18405419327840633 1104 3 0.27195475720657925 1104 4 0.3065564511874541 1104 5 0.31634960412579005 1104 6 0.3628669903797217 1104 7 0.39772550718272726 1104 8 0.3287209252874135 1104 9 0.40855146623714944 1104 10-11 0.41740562856359276 1104 12-13 0.3772419261227782 1104 14-15 0.5002950990348367 1104 16-17 0.5919534929689689 1104 18-19 0.5072341814077888 1104 20-21 0.4415183744774467 1104 22-23 0.5934176966950702 1104 24-25 0.6157098841080284 1104 26-27 0.46516796744286637 1104 28-29 0.5962923331558017 1104 30-31 0.488528421450944 1104 32-33 0.537249400083617 1104 34-35 0.44650094115625194 1104 36-37 0.4022363964113822 1104 38-39 0.2749022663790157 1104 40-41 0.35890342973947753 1104 42-43 0.45120454054230663 1104 44-45 0.4344381751681041 1104 46-47 0.46704666554580143 1104 48-49 0.4474258673971363 1104 50-51 0.3671585025545667 1104 52-53 0.40490853035453256 1104 54-55 0.5323271332062518 1104 56-57 0.41825885314963074 1104 58-59 0.3877781004587604 1104 60-61 0.33453716807073164 1104 62-63 0.48019483556910103 1104 64-65 0.47174409296094666 1104 66-67 0.6211434889222076 1104 68-69 0.39759491732740315 1104 70-71 0.33675442080294715 1104 72-73 0.5663776426248432 1104 74-75 0.5214165519610461 1104 76-77 0.45832594222745726 1104 78-79 0.33484248186582377 1104 80-81 0.5553623823811868 1104 82-83 0.5508776243071765 1104 84-85 0.3886271700947468 1104 86-87 0.4021139005267109 1104 88-89 0.39938414348297613 1104 90-91 0.700887807808872 1104 92-93 0.4956212490166152 1104 94-95 0.24209691947504197 1104 96-97 0.4750831651055982 1104 98-99 0.6283336133030879 1104 100-101 0.7339622040113021 1105 1 0.3166602904533704 1105 2 0.23927905706309005 1105 3 0.1838974245829661 1105 4 0.11500101051415612 1105 5 0.21028512583647085 1105 6 0.21623081020078416 1105 7 0.2464628574018093 1105 8 0.3395673289769121 1105 9 0.22296039255626 1105 10-11 0.3054876668169513 1105 12-13 0.2624087730157143 1105 14-15 0.19779910093347297 1105 16-17 0.2249063774363549 1105 18-19 0.2932932760096847 1105 20-21 0.2368977651675479 1105 22-23 0.35264261105820793 1105 24-25 0.3274163403748034 1105 26-27 0.3247063709582534 1105 28-29 0.33384863512182505 1105 30-31 0.3788742102961038 1105 32-33 0.41413986101139955 1105 34-35 0.22748298021205926 1105 36-37 0.389887715354007 1105 38-39 0.40523321725640216 1105 40-41 0.395258639308139 1105 42-43 0.5222033166895983 1105 44-45 0.5700646659255071 1105 46-47 0.3553932680232208 1105 48-49 0.5364292359708429 1105 50-51 0.45422140926430643 1105 52-53 0.3926377838870607 1105 54-55 0.6396930340389204 1105 56-57 0.6418405469588464 1105 58-59 0.6562095093872671 1105 60-61 0.6126900964051956 1105 62-63 0.4499928111178306 1105 64-65 0.5374463565656455 1105 66-67 0.4907465185716333 1105 68-69 0.41514576265630154 1105 70-71 0.4085112658551182 1105 72-73 0.5533795877068499 1105 74-75 0.5061034384997143 1105 76-77 0.759161848455971 1105 78-79 0.6338536730784021 1105 80-81 0.7342887720059892 1105 82-83 0.6596208153650984 1105 84-85 0.5962944017973193 1105 86-87 0.7061674961806617 1105 88-89 0.6835292812764386 1105 90-91 0.8246207020174658 1105 92-93 0.5491089374183176 1105 94-95 0.6283875564061141 1105 96-97 0.6531764692233892 1105 98-99 0.6597943847266485 1105 100-101 0.6462003176489368 1106 1 0.17351325673476836 1106 2 0.050624526444707385 1106 3 0.07406099818571832 1106 4 0.08482015082670102 1106 5 0.1251914212996823 1106 6 0.0655627191321031 1106 7 0.05654676470115305 1106 8 0.06793959178982334 1106 9 0.10566196065108358 1106 10-11 0.023359438425892165 1106 12-13 0.08698453921336835 1106 14-15 0.04565202963642179 1106 16-17 0.10715432001649106 1106 18-19 0.14204808739273034 1106 20-21 0.024362132749168097 1106 22-23 0.07262414393666461 1106 24-25 0.09522181287006504 1106 26-27 -0.05831998226838664 1106 28-29 0.0012176608201173167 1106 30-31 0.10538634008131709 1106 32-33 0.2140990615568228 1106 34-35 0.07162101120891151 1106 36-37 0.17839085852251912 1106 38-39 0.16809444005900076 1106 40-41 0.1964471918401216 1106 42-43 0.2544220017661303 1106 44-45 0.20408013750815002 1106 46-47 0.3579539706327779 1106 48-49 0.48430256440108366 1106 50-51 0.382400948337839 1106 52-53 0.2490263879551904 1106 54-55 0.3497806991827659 1106 56-57 0.3408130449054525 1106 58-59 0.33406828959093815 1106 60-61 0.312846315369427 1106 62-63 0.19826286056032671 1106 64-65 0.1835701399634857 1106 66-67 0.26095164319786335 1106 68-69 0.29015448622389073 1106 70-71 0.38779480595937343 1106 72-73 0.3703484029658455 1106 74-75 0.2820339076397502 1106 76-77 0.3360151368383235 1106 78-79 0.42279702088838533 1106 80-81 0.4370486403740621 1106 82-83 0.42968636290988727 1106 84-85 0.5461191251931226 1106 86-87 0.3890805772373689 1106 88-89 0.3018181736249268 1106 90-91 0.393630832092569 1106 92-93 0.2731516265318419 1106 94-95 0.36577864601997234 1106 96-97 0.3408485919223807 1106 98-99 0.2745052722758352 1106 100-101 0.33874153315057853 1107 1 0.13320805911128275 1107 2 0.24434623785642984 1107 3 0.3318870972995711 1107 4 0.24536742521400612 1107 5 0.18594938222359048 1107 6 0.2914223335745305 1107 7 0.2837295829891815 1107 8 0.296513694973946 1107 9 0.2840010018510597 1107 10-11 0.3029048515259092 1107 12-13 0.33988813523014727 1107 14-15 0.4617817678039984 1107 16-17 0.2175085967829773 1107 18-19 0.38104199535444394 1107 20-21 0.40826230161448507 1107 22-23 0.39854867852366027 1107 24-25 0.36957385755069083 1107 26-27 0.3125510539859846 1107 28-29 0.4297566293192574 1107 30-31 0.4002109645109826 1107 32-33 0.35996575156556077 1107 34-35 0.28762200324707266 1107 36-37 0.22322502645657494 1107 38-39 0.2903884310552769 1107 40-41 0.43688064929980186 1107 42-43 0.07318501770803465 1107 44-45 0.38185203924287947 1107 46-47 0.4610522662966261 1107 48-49 0.4554832478618849 1107 50-51 0.34829440878822027 1107 52-53 0.35154307693056097 1107 54-55 0.49514711504370723 1107 56-57 0.44085754073488204 1107 58-59 0.6043432519336136 1107 60-61 0.6895568821563245 1107 62-63 0.7175490064312626 1107 64-65 0.4915824285930981 1107 66-67 0.5085225477883526 1107 68-69 0.5697287121970547 1107 70-71 0.7606462713431981 1107 72-73 0.5790562124702063 1107 74-75 0.6017462895160932 1107 76-77 0.6468261645096298 1107 78-79 0.5885810653647603 1107 80-81 0.4507080089952673 1107 82-83 0.5683909264070159 1107 84-85 0.544899137082993 1107 86-87 0.46771589258028357 1107 88-89 0.6040796802342605 1107 90-91 0.6641612199478821 1107 92-93 0.3742936861538233 1107 94-95 0.5439259661188345 1107 96-97 0.7603850572271824 1107 98-99 0.8035229964478923 1107 100-101 0.8564550067551941 1108 1 0.005473862748669234 1108 2 0.12317974121591035 1108 3 0.10892066866951211 1108 4 0.048984878105038376 1108 5 0.15140475915396934 1108 6 0.04197872948920889 1108 7 0.1161201506419971 1108 8 0.06970584797402779 1108 9 0.1416621455993763 1108 10-11 0.08595441707978324 1108 12-13 0.044380929639210365 1108 14-15 0.051905593744123735 1108 16-17 0.2113372338768187 1108 18-19 0.17375901321305065 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20 10841.0 21 11140.0 22 11507.0 23 12892.0 24 14333.0 25 16839.0 26 19883.0 27 23662.0 28 28412.0 29 34079.0 30 41595.0 31 51411.0 32 65294.0 33 85675.0 34 118951.0 35 178421.0 36 289788.0 37 479007.0 38 483715.0 39 89649.0 40 114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.872795184231194 17.732353834622415 12.544419859114678 23.85043112203171 2 15.959443409262017 19.021312379723682 40.08720987689814 24.93203433411616 3 20.231060936156602 23.63799465089306 27.889968672596744 28.240975740353598 4 12.644580656579224 14.925215585199611 36.025299762023046 36.404903996198115 5 14.25031129222659 37.32771120705919 33.27417433442758 15.147803166286646 6 34.383715534825306 34.441660015029626 16.807986815609436 14.366637634535628 7 30.837582604758467 31.431896666343256 19.404032944879855 18.32648778401843 8 27.643193821111772 34.69201014661563 18.660510178733702 19.004285853538892 9 27.29941106973276 16.13866589999416 19.415202576738377 37.146720453534705 10-11 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26.071361419887566 27.71852197126043 22.856731101586046 36-37 23.34684407916366 26.035808177344506 27.553843477373995 23.063504266117842 38-39 23.06493992935254 26.112122097581942 27.890538182006896 22.932399791058618 40-41 23.239972060451432 25.88411857833286 28.034470812988953 22.84143854822675 42-43 23.118850880314625 26.017149780420617 27.70234057787706 23.161658761387695 44-45 23.09099961586396 26.246760685955923 27.576724515778693 23.085515182401423 46-47 23.094974659550484 26.471842916749615 27.067649317250147 23.36553310644975 48-49 23.547216234213288 26.389422480051465 26.974594539688272 23.088766746046975 50-51 23.30293380506105 26.46571734172763 26.867078706017644 23.364270147193675 52-53 23.54338525379449 26.487628881337756 26.911759593954095 23.05722627091366 54-55 23.2440027959004 26.303040124149895 26.998787796170102 23.454169283779603 56-57 23.282866375176088 26.866602663315742 26.763578933780003 23.086952027728167 58-59 23.46906006091059 26.342103570570185 26.935750499031275 23.253085869487947 60-61 23.41919381118518 26.410965828823663 27.219456492794308 22.950383867196845 62-63 23.318003839481943 26.439235921880737 27.112578241235642 23.13018199740168 64-65 23.10959960583297 26.34387281748401 27.06733995258891 23.479187624094113 66-67 23.15891440071429 26.767830956308735 26.72626771824818 23.346986924728796 68-69 23.06900956057987 26.465487997194053 27.140228985356124 23.325273456869954 70-71 23.04135714419499 26.55885028353195 27.070153311927115 23.329639260345942 72-73 23.193986628024277 26.55255246038305 26.940495926706443 23.31296498488623 74-75 23.01521289223718 26.657377786635795 26.799080983218847 23.528328337908178 76-77 23.19268590968784 26.690783069950886 26.794732305892406 23.321798714468873 78-79 23.262793243136503 26.599316189165094 26.8338655623385 23.304025005359904 80-81 22.991599475864348 27.2313116436022 26.693476786053623 23.08361209447983 82-83 23.354121430381767 27.070002241079184 26.392465099084816 23.183411229454233 84-85 23.31649869644031 26.618639278672894 26.36783493539977 23.697027089487026 86-87 23.22609329544762 26.547901427936278 26.844344338696462 23.38166093791964 88-89 23.377434502833687 26.671501588617215 26.626154444832682 23.324909463716416 90-91 23.27081867663939 26.89027325345642 26.724823826162503 23.114084243741686 92-93 23.18584861916219 26.591630768841785 26.825551696254056 23.39696891574196 94-95 23.209057893496766 26.600848032166365 26.723913802955746 23.466180271381127 96-97 23.267298808743252 26.564136488899457 26.67509903824936 23.493465664107923 98-99 23.075758611486556 26.454860295645453 26.62731224699884 23.842068845869154 100-101 23.16563150361269 26.597824262344794 26.530249865076826 23.706294368965686 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 174.0 1 126.5 2 187.0 3 333.5 4 293.5 5 272.5 6 548.5 7 1109.0 8 1782.0 9 2039.5 10 1813.0 11 1717.5 12 1934.0 13 2232.0 14 2533.0 15 2725.0 16 2869.5 17 3087.0 18 3114.5 19 3128.5 20 3329.5 21 3511.0 22 3882.0 23 4574.5 24 5491.5 25 6812.5 26 8630.5 27 10806.5 28 12834.5 29 15344.0 30 18248.5 31 21669.0 32 26293.5 33 30928.0 34 35819.5 35 39901.0 36 44626.5 37 49972.5 38 53518.5 39 56143.5 40 58604.5 41 62951.5 42 67776.0 43 71806.0 44 74613.5 45 77007.5 46 78975.5 47 81587.0 48 90215.0 49 93660.0 50 90293.5 51 87690.5 52 84731.5 53 83896.5 54 83244.5 55 78707.0 56 71228.5 57 64640.5 58 59261.5 59 53029.0 60 45813.0 61 37997.5 62 31648.0 63 25849.5 64 20948.0 65 17188.5 66 12598.0 67 10065.0 68 7023.5 69 3827.0 70 2914.5 71 2554.5 72 2285.5 73 2040.0 74 1672.5 75 1362.0 76 923.0 77 473.0 78 256.0 79 167.5 80 118.5 81 71.5 82 44.0 83 36.5 84 35.0 85 33.0 86 21.0 87 19.0 88 21.5 89 17.0 90 15.5 91 15.0 92 23.0 93 24.0 94 19.0 95 18.0 96 17.5 97 15.5 98 11.0 99 9.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04635310646661768 2 0.011857771421692894 3 0.00997130778642357 4 0.005479727702448989 5 0.00826450735451323 6 0.005344980299929752 7 0.005569559304128481 8 0.0101958867906223 9 0.014283224667039168 10-11 0.002290705842827036 12-13 0.009050533869208783 14-15 0.008915786466689545 16-17 0.018842178452273366 18-19 0.011723024019173656 20-21 0.004064879975996996 22-23 0.009903934085163952 24-25 0.021132884295100404 26-27 0.007074238632259965 28-29 0.017494704427080995 30-31 0.014238308866199421 32-33 0.011992518824212131 34-35 0.26637315688011254 36-37 0.47150361931523166 38-39 0.4696171556799624 40-41 0.4891555290452518 42-43 0.4794537160638667 44-45 0.49528653585987703 46-47 0.5010357583673645 48-49 0.5081324549000444 50-51 0.5188224154999038 52-53 0.5061561596630956 54-55 0.49414118293846354 56-57 0.4886389973355947 58-59 0.48713431800746326 60-61 0.4841025014507804 62-63 0.48169950610585394 64-65 0.4978242786073227 66-67 0.4710544613068342 68-69 0.49827343661572016 70-71 0.47954354766554613 72-73 0.4877406813187998 74-75 0.5028099325005345 76-77 0.4888411184393735 78-79 0.4879203445221587 80-81 0.4909746189792614 82-83 0.4912665716847198 84-85 0.49030088196666527 86-87 0.49196276659773586 88-89 0.5054824226504994 90-91 0.530635271120757 92-93 0.5181935942881475 94-95 0.4898517239582678 96-97 0.4637556436703755 98-99 0.454480530796968 100-101 0.46317173825945884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2226388.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.310852505006686 #Duplication Level Percentage of deduplicated Percentage of total 1 72.39419571501965 34.25031115691882 2 14.299077146536328 13.530030596749842 3 5.405152067020503 7.671670566298169 4 2.5819842329398948 4.886235008594888 5 1.409160004780361 3.333428057105908 6 0.895110949320432 2.540907725934928 7 0.5670578195770478 1.8779592204674278 8 0.39773370675628206 1.5053696589292833 9 0.2769747095971561 1.17935186700313 >10 1.5392853311557229 13.360031844932388 >50 0.1373468276708439 4.530306943358858 >100 0.08977670811815243 7.888022485069988 >500 0.004763187671804461 1.4874032381908404 >1k 0.0022863300824661147 1.67453426797594 >5k 9.526375343608811E-5 0.2844373624696172 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6311 0.2834636190996358 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4199 0.18860144772609266 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2936 0.1318727912654937 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 2420 0.10869623803218485 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2347 0.10541738457088341 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.4915800839745816E-5 0.0 14-15 0.0 0.0 0.0 6.737370125961873E-5 0.0 16-17 0.0 0.0 0.0 1.1228950209936454E-4 0.0 18-19 0.0 0.0 0.0 2.2457900419872907E-4 0.0 20-21 0.0 0.0 0.0 4.7161590881733106E-4 0.0 22-23 0.0 0.0 0.0 0.001167810821833391 0.0 24-25 0.0 0.0 0.0 0.0019313794361090699 0.0 26-27 0.0 0.0 0.0 0.0022682479424071637 0.0 28-29 0.0 0.0 0.0 0.002739863851224495 0.0 30-31 0.0 0.0 0.0 0.004087337876416869 0.0 32-33 0.0 0.0 0.0 0.007231443935199075 0.0 34-35 0.0 0.0 0.0 0.011026829106157597 0.0 36-37 0.0 0.0 0.0 0.017202751721622646 0.0 38-39 0.0 0.0 0.0 0.028341870329879608 0.0 40-41 0.0 0.0 0.0 0.043321289909934835 0.0 42-43 0.0 0.0 0.0 0.05863757799628816 0.0 44-45 0.0 0.0 0.0 0.07599753502084991 0.0 46-47 0.0 0.0 0.0 0.09585031899201757 0.0 48-49 0.0 0.0 0.0 0.11970060923792258 0.0 50-51 0.0 0.0 0.0 0.1466276318413502 0.0 52-53 0.0 0.0 0.0 0.17420593355695413 0.0 54-55 0.0 0.0 0.0 0.20328891460068954 0.0 56-57 0.0 0.0 0.0 0.23818849185317204 0.0 58-59 0.0 0.0 0.0 0.2789271232148215 0.0 60-61 0.0 0.0 0.0 0.32521285598017957 0.0 62-63 0.0 0.0 0.0 0.3698591620148869 0.0 64-65 0.0 0.0 0.0 0.41616735268066485 0.0 66-67 0.0 0.0 0.0 0.46934766087492386 0.0 68-69 0.0 0.0 0.0 0.5338018350799592 0.0 70-71 0.0 0.0 0.0 0.6056671164235524 0.0 72-73 0.0 0.0 0.0 0.6829447517683351 0.0 74-75 0.0 0.0 0.0 0.7609859557273935 0.0 76-77 0.0 0.0 0.0 0.8463259773229105 0.0 78-79 0.0 0.0 0.0 0.946600502697643 0.0 80-81 0.0 0.0 0.0 1.0650659274124725 0.0 82-83 0.0 0.0 0.0 1.187079700393642 0.0 84-85 0.0 0.0 0.0 1.3132481849524882 0.0 86-87 0.0 0.0 0.0 1.4455925921267991 0.0 88-89 0.0 0.0 0.0 1.597542746367659 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7570 0.0 36.521935 1 GGTATCA 4635 0.0 35.237587 1 ATCAACG 9025 0.0 30.203411 3 TCAACGC 9070 0.0 30.053556 4 GTACATG 9710 0.0 29.252893 1 CAACGCA 9365 0.0 29.106861 5 AACGCAG 9565 0.0 28.49505 6 TATCAAC 9665 0.0 28.398607 2 TACATGG 9865 0.0 28.254599 2 ACATGGG 9955 0.0 26.906416 3 GTACCTG 1810 0.0 24.84745 1 ACGCAGA 11015 0.0 24.52921 7 CGCAGAG 11195 0.0 24.135355 8 CATGGGG 4620 0.0 22.535198 4 GCAGAGT 12105 0.0 21.734602 9 CATGGGA 5440 0.0 21.487144 4 TACCTGG 1960 0.0 21.246994 2 GATGTGC 1495 0.0 20.890287 8 GGACCGT 230 9.402174E-8 20.573305 6 AGTACAT 6880 0.0 20.566181 2 >>END_MODULE