FastQCFastQC Report
Thu 26 May 2016
SRR936340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936340_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226388
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64760.2908747262381939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46900.21065510593840786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31080.13959830900993No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG26320.11821838781021098No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG25760.11570310296318521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54050.037.314631
GTATCAA83050.033.6207541
ACGACCG650.005885840429.1893835
TCAACGC99150.027.7468434
ATCAACG99250.027.7188853
GTACATG100900.027.2958341
CAACGCA101100.027.2585815
TACATGG101950.026.7122362
AACGCAG104300.026.4677456
TATCAAC104850.026.2901652
ACATGGG100250.026.2595253
GTACCTG17000.023.224061
ACGCAGA120550.022.7425377
CATGGGA54600.022.3264054
CGCAGAG122450.022.312188
TACCTGG17000.021.7686372
AGTACAT72950.020.8768672
CATGGGG46050.020.6005424
GCAGAGT132900.020.2722369
GAGTACT65450.020.25675812-13
Produced by FastQC (version 0.11.5)