##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936340_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2226388 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47767325371858 34.0 31.0 34.0 31.0 34.0 2 32.60780061696344 34.0 31.0 34.0 31.0 34.0 3 32.68896975729298 34.0 31.0 34.0 31.0 34.0 4 36.177019459321556 37.0 35.0 37.0 35.0 37.0 5 36.14077510299193 37.0 35.0 37.0 35.0 37.0 6 36.17864406383793 37.0 37.0 37.0 35.0 37.0 7 36.13192938517455 37.0 36.0 37.0 35.0 37.0 8 36.1079479407902 37.0 36.0 37.0 35.0 37.0 9 37.875354161089625 39.0 38.0 39.0 35.0 39.0 10-11 37.80753961124476 39.0 38.0 39.0 35.0 39.0 12-13 37.7526850665742 39.0 38.0 39.0 35.0 39.0 14-15 39.09505036857907 40.0 38.0 41.0 36.0 41.0 16-17 39.026337053559395 40.0 38.0 41.0 35.5 41.0 18-19 39.05213197340266 40.0 38.5 41.0 36.0 41.0 20-21 39.011133953291164 40.0 38.5 41.0 35.0 41.0 22-23 38.89752280375208 40.0 38.0 41.0 35.0 41.0 24-25 39.04051405235745 40.0 39.0 41.0 35.0 41.0 26-27 38.96647349877919 40.0 38.0 41.0 35.0 41.0 28-29 38.87261631844943 40.0 38.0 41.0 35.0 41.0 30-31 38.74958879584331 40.0 38.0 41.0 35.0 41.0 32-33 38.598284081660516 40.0 38.0 41.0 34.5 41.0 34-35 38.460555617439546 40.0 38.0 41.0 34.0 41.0 36-37 38.307168157571816 40.0 38.0 41.0 34.0 41.0 38-39 38.17792720765652 40.0 38.0 41.0 33.5 41.0 40-41 38.02733845133912 40.0 38.0 41.0 33.0 41.0 42-43 38.043715201483295 40.0 38.0 41.0 33.0 41.0 44-45 37.9035197369012 40.0 38.0 41.0 33.0 41.0 46-47 37.68017007817146 40.0 37.0 41.0 33.0 41.0 48-49 37.45060272513147 40.0 37.0 41.0 32.5 41.0 50-51 37.244520496876554 40.0 37.0 41.0 32.0 41.0 52-53 37.02568667276324 40.0 36.0 41.0 31.5 41.0 54-55 36.82450386006393 39.5 36.0 41.0 31.0 41.0 56-57 36.585747632488136 39.0 35.0 41.0 31.0 41.0 58-59 36.33137463011838 39.0 35.0 41.0 30.5 41.0 60-61 36.05643333507008 39.0 35.0 40.5 30.0 41.0 62-63 35.76622762968539 38.0 35.0 40.0 30.0 41.0 64-65 35.390808789842566 37.5 35.0 40.0 29.0 41.0 66-67 35.04753596408173 37.0 34.0 40.0 29.0 41.0 68-69 34.694305979011745 36.5 34.0 39.0 28.5 41.0 70-71 34.31978410771168 36.0 34.0 39.0 28.0 40.5 72-73 33.91029798040593 35.5 34.0 38.5 27.5 40.0 74-75 33.481339281383114 35.0 33.5 37.0 27.0 39.5 76-77 32.750473412540856 35.0 32.5 37.0 26.0 39.0 78-79 32.80353491844189 35.0 33.0 36.0 26.0 39.0 80-81 32.63020282179028 35.0 33.0 36.0 26.0 37.5 82-83 32.34978696435662 35.0 33.0 36.0 26.0 37.0 84-85 32.06240758573977 35.0 33.0 35.0 26.0 37.0 86-87 31.654577728589985 35.0 32.5 35.0 25.0 36.0 88-89 31.167801389515216 35.0 32.0 35.0 23.5 36.0 90-91 30.960354843809796 34.5 32.0 35.0 22.5 36.0 92-93 30.67621995806661 34.0 31.0 35.0 20.0 35.0 94-95 30.52452447641651 34.0 31.0 35.0 19.5 35.0 96-97 30.27152432549942 34.0 31.0 35.0 17.0 35.0 98-99 29.824047066369385 34.0 31.0 35.0 2.0 35.0 100-101 26.48262140291809 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19194380381500054 1101 2 0.18132294163018514 1101 3 0.22230393663346604 1101 4 0.14422562842867848 1101 5 0.13414943397564372 1101 6 0.15699851848835777 1101 7 0.16603267275424827 1101 8 0.10822780631743001 1101 9 0.23808367017298337 1101 10-11 0.13942227645904381 1101 12-13 0.2599768531172728 1101 14-15 0.3728342470395347 1101 16-17 0.424524990781201 1101 18-19 0.3564951901736819 1101 20-21 0.3235616392834899 1101 22-23 0.3957630545746369 1101 24-25 0.34114311584987433 1101 26-27 0.3350633787016264 1101 28-29 0.3375038374930739 1101 30-31 0.33194001461304623 1101 32-33 0.4589676316084095 1101 34-35 0.4803918964923142 1101 36-37 0.42630660452426383 1101 38-39 0.3361628887204873 1101 40-41 0.3551252701170782 1101 42-43 0.2908875138422218 1101 44-45 0.32382512844461786 1101 46-47 0.29842761727548606 1101 48-49 0.3263860398573115 1101 50-51 0.3932587857825496 1101 52-53 0.3705076306852746 1101 54-55 0.39350892596571896 1101 56-57 0.3611293438047838 1101 58-59 0.19143349228313866 1101 60-61 0.3025641890494555 1101 62-63 0.22816584780738225 1101 64-65 0.24615822434987678 1101 66-67 0.22731620982698075 1101 68-69 0.2613492350206954 1101 70-71 0.3102764244459024 1101 72-73 0.2894040771715254 1101 74-75 0.07509068372966254 1101 76-77 0.055714106659102924 1101 78-79 0.0649525928422392 1101 80-81 0.18135278537162947 1101 82-83 0.14173049202334198 1101 84-85 0.3453068917475193 1101 86-87 0.2826111735864494 1101 88-89 0.19948588504119869 1101 90-91 0.3106914764942914 1101 92-93 0.32535926124550585 1101 94-95 0.3235679980151467 1101 96-97 0.33733396757808976 1101 98-99 0.29542434107478854 1101 100-101 0.7548299316968503 1102 1 0.049479561476296396 1102 2 0.16937144375333446 1102 3 0.11678719949585314 1102 4 0.1323890028843664 1102 5 0.10763488339176064 1102 6 0.14458355187030492 1102 7 0.16406472133914463 1102 8 0.20583639250695995 1102 9 0.12750087746475458 1102 10-11 0.08665196965706201 1102 12-13 0.15884290667165857 1102 14-15 0.14724567887938633 1102 16-17 0.23112760307885338 1102 18-19 0.3233715010580127 1102 20-21 0.21341260238055781 1102 22-23 0.270945643218667 1102 24-25 0.27141229382558407 1102 26-27 0.2798401629236338 1102 28-29 0.2318129347981639 1102 30-31 0.20370853386358334 1102 32-33 0.1895376265306794 1102 34-35 0.13085303064568876 1102 36-37 0.049672459373390154 1102 38-39 0.09955732104688764 1102 40-41 0.1322263858908883 1102 42-43 0.11211950236454271 1102 44-45 0.11268248565937 1102 46-47 0.15860716299653888 1102 48-49 0.29618721786910385 1102 50-51 0.1653633530322054 1102 52-53 0.18480257720464266 1102 54-55 0.03620835149793322 1102 56-57 0.16514945097297584 1102 58-59 0.07453922099023913 1102 60-61 0.08621408895400151 1102 62-63 0.23934454405681294 1102 64-65 0.22506986603733736 1102 66-67 0.1503452473241751 1102 68-69 0.002900149462824686 1102 70-71 0.03765838052275683 1102 72-73 0.10023361989473756 1102 74-75 0.11501677810750266 1102 76-77 -0.04297568551483266 1102 78-79 0.08927634499178083 1102 80-81 0.15251412608296988 1102 82-83 0.20915935739774483 1102 84-85 0.17623471531211266 1102 86-87 0.14758272610326628 1102 88-89 0.1462897812560371 1102 90-91 0.25247693742417 1102 92-93 0.2434072699216827 1102 94-95 0.12235332258387288 1102 96-97 -0.020232431548347307 1102 98-99 0.2759372977718968 1102 100-101 0.841394060436631 1103 1 -0.029429923366350863 1103 2 -0.02578613840298516 1103 3 0.11169285585577171 1103 4 0.06834942389282617 1103 5 0.07294639942833214 1103 6 0.13843133800290985 1103 7 0.1522858530889053 1103 8 0.14128293890082944 1103 9 0.1331960830847052 1103 10-11 0.20028226660335946 1103 12-13 0.17714154286556294 1103 14-15 0.2384338847429035 1103 16-17 0.2032990466069151 1103 18-19 0.08692776786150347 1103 20-21 0.09534867627463228 1103 22-23 0.15093813374223686 1103 24-25 0.10503960983172789 1103 26-27 0.16965975891795892 1103 28-29 0.12924327609503905 1103 30-31 0.06344776911220862 1103 32-33 0.13441801760509975 1103 34-35 0.10615627898760494 1103 36-37 0.04816601997138292 1103 38-39 0.009974226820617105 1103 40-41 0.12114224807589125 1103 42-43 0.006508921169498194 1103 44-45 -0.03374100934235713 1103 46-47 0.06425742081669483 1103 48-49 0.025520837525029094 1103 50-51 0.1237147113593835 1103 52-53 -0.010298917575859434 1103 54-55 0.005299922967537896 1103 56-57 0.0529305564863094 1103 58-59 0.12765253774317387 1103 60-61 0.07774658301938331 1103 62-63 0.12349217433921922 1103 64-65 0.009585673589093346 1103 66-67 -0.09885810420812646 1103 68-69 -0.08692209510202531 1103 70-71 -0.11093058054689209 1103 72-73 -0.02485563952858172 1103 74-75 -0.04757286431989627 1103 76-77 -0.04394981417407706 1103 78-79 -0.06622876077705797 1103 80-81 0.00497070121122789 1103 82-83 0.054149560283441645 1103 84-85 0.16757363003177517 1103 86-87 0.03758590438789611 1103 88-89 0.056269672893151323 1103 90-91 0.1439411139498361 1103 92-93 0.2327704029624975 1103 94-95 0.21890358544151312 1103 96-97 0.06185157279945841 1103 98-99 -0.05633665062875437 1103 100-101 0.21092781335200428 1104 1 0.04765145701113482 1104 2 0.11957036873480575 1104 3 0.05820012192047841 1104 4 0.017161125455096737 1104 5 0.1188291136720423 1104 6 0.04834933963829968 1104 7 0.09403811445142907 1104 8 0.1333997673919214 1104 9 0.01978953533885175 1104 10-11 0.10307178552164231 1104 12-13 0.241831866424711 1104 14-15 0.26533657201854055 1104 16-17 0.20436046527093765 1104 18-19 0.2819956420637766 1104 20-21 0.2778671611824919 1104 22-23 0.1998160859386573 1104 24-25 0.26520014060142927 1104 26-27 0.3103172247783803 1104 28-29 0.32660489615192745 1104 30-31 0.19813827628168923 1104 32-33 0.061015257765660635 1104 34-35 0.1946173502004953 1104 36-37 0.318073467785176 1104 38-39 0.32447773314926565 1104 40-41 0.3871860816484656 1104 42-43 0.42599930941984354 1104 44-45 0.39297960187381875 1104 46-47 0.43743964703418925 1104 48-49 0.46793145513235146 1104 50-51 0.6068595338874232 1104 52-53 0.5526598095869772 1104 54-55 0.41742363810389804 1104 56-57 0.444745584916987 1104 58-59 0.4869486662563247 1104 60-61 0.48492438900664325 1104 62-63 0.43276014507525673 1104 64-65 0.33834161797376794 1104 66-67 0.338673131557357 1104 68-69 0.522157913982916 1104 70-71 0.5157927470961994 1104 72-73 0.3140403743915954 1104 74-75 0.4362501846127671 1104 76-77 0.384035318922038 1104 78-79 0.43387851293389446 1104 80-81 0.5957578387285807 1104 82-83 0.4447332676075604 1104 84-85 0.4741691900319793 1104 86-87 0.5583090977187624 1104 88-89 0.669791039980268 1104 90-91 0.7192111657707088 1104 92-93 0.7348522423658288 1104 94-95 0.8245585071669161 1104 96-97 0.7327577099372675 1104 98-99 0.7011613793329303 1104 100-101 0.8848101986062353 1105 1 0.004249306989116519 1105 2 0.05787194909265736 1105 3 0.045518790257858655 1105 4 0.02938287404467843 1105 5 -0.02498342701260725 1105 6 0.026262603910971904 1105 7 0.09002661077099816 1105 8 0.06128072710836818 1105 9 0.021867385793434835 1105 10-11 0.03550261766123697 1105 12-13 0.05663143835590034 1105 14-15 -0.02064069734311147 1105 16-17 0.02287244713660641 1105 18-19 0.01190110169183356 1105 20-21 0.0163096803494156 1105 22-23 -0.04042622376537963 1105 24-25 -0.006524460685945144 1105 26-27 0.027671446365019392 1105 28-29 -0.04113670046884721 1105 30-31 0.009178750474156061 1105 32-33 -0.08300093414845122 1105 34-35 -0.11358246290238583 1105 36-37 -0.06475376083405848 1105 38-39 -0.061155019123546595 1105 40-41 -7.470176460415701E-4 1105 42-43 0.020813121131332935 1105 44-45 -0.1439973822545184 1105 46-47 0.016743502693010726 1105 48-49 -0.07582097500432639 1105 50-51 -0.10803287868783684 1105 52-53 -0.07011136448728195 1105 54-55 -0.08680160818035176 1105 56-57 0.026251427093242796 1105 58-59 0.07378773898790314 1105 60-61 0.048539197113683485 1105 62-63 0.01199105103993503 1105 64-65 -0.01778760316909711 1105 66-67 -0.024537681143577572 1105 68-69 -0.12179201529236394 1105 70-71 -0.07101969899701999 1105 72-73 -0.03203260983206491 1105 74-75 0.05441434233680553 1105 76-77 0.04580600739450347 1105 78-79 0.017416490423073583 1105 80-81 -4.4189558863649836E-4 1105 82-83 0.035006447190490064 1105 84-85 0.0719480420615568 1105 86-87 0.08469609633247899 1105 88-89 0.10055205362113284 1105 90-91 0.19000514781000888 1105 92-93 0.33276070168530936 1105 94-95 0.04874775034545209 1105 96-97 -0.06480810466886666 1105 98-99 0.006524786745057298 1105 100-101 0.29727238938767897 1106 1 -0.004463523902515476 1106 2 0.011806143867111984 1106 3 -0.038318720545944984 1106 4 -0.007513790599908532 1106 5 -0.0421621447286995 1106 6 -0.08082522765170097 1106 7 -0.006947250736324406 1106 8 0.040575003911200724 1106 9 0.011758784087376739 1106 10-11 0.07724153336204864 1106 12-13 0.10454663991057345 1106 14-15 0.09480296988365922 1106 16-17 0.06335785327907217 1106 18-19 0.17416482721886695 1106 20-21 0.07006564592756348 1106 22-23 0.05215364385112764 1106 24-25 0.11655299922590956 1106 26-27 -0.002673147975670531 1106 28-29 0.05456486225762802 1106 30-31 0.04527016272580653 1106 32-33 0.03108722488352811 1106 34-35 0.10516703381486536 1106 36-37 0.07077826942195742 1106 38-39 0.1297461656711434 1106 40-41 -0.055383392507131646 1106 42-43 -0.05102881319228203 1106 44-45 -0.021284970094136213 1106 46-47 0.09270832926848271 1106 48-49 0.05000776391673867 1106 50-51 0.1719345891154802 1106 52-53 0.09707782150992017 1106 54-55 0.09824539171765423 1106 56-57 0.24652553500779817 1106 58-59 0.29284054743982324 1106 60-61 0.2306738675238833 1106 62-63 0.09927504208553017 1106 64-65 0.07193093427782316 1106 66-67 0.04825176682906829 1106 68-69 0.10440069771032512 1106 70-71 -0.04340491026840709 1106 72-73 -0.0681428113062168 1106 74-75 0.08443951989496412 1106 76-77 0.0908920888753002 1106 78-79 -0.06430857493658237 1106 80-81 -0.10463887106065073 1106 82-83 -0.02473522784185178 1106 84-85 0.15558692394176177 1106 86-87 0.10634128065475323 1106 88-89 0.24296093931287288 1106 90-91 0.363590721981808 1106 92-93 0.2748120553145803 1106 94-95 0.25941519721221695 1106 96-97 0.37366792833574536 1106 98-99 0.27911077427975073 1106 100-101 0.5887361771875668 1107 1 -0.006732568056719401 1107 2 0.11283477559604194 1107 3 0.03302511428942978 1107 4 0.072246320955756 1107 5 0.0730397459793366 1107 6 0.07413356507675672 1107 7 0.08776127118759547 1107 8 0.07698645173983465 1107 9 0.09726177539852898 1107 10-11 0.15110740639818232 1107 12-13 0.011145274156078244 1107 14-15 0.11660750675623888 1107 16-17 0.13570187169013792 1107 18-19 0.13877714818698195 1107 20-21 0.1447207728561679 1107 22-23 0.08939884140166754 1107 24-25 0.1719323140144482 1107 26-27 0.247418335935933 1107 28-29 0.1694299949123419 1107 30-31 0.10077931780349303 1107 32-33 0.036564345395497355 1107 34-35 0.20300011123639905 1107 36-37 0.2748765055148894 1107 38-39 0.365246056863036 1107 40-41 0.23670206380003123 1107 42-43 0.09113001260411124 1107 44-45 0.12819378436304874 1107 46-47 0.22601953626040938 1107 48-49 0.05635644344829416 1107 50-51 0.1827659195286202 1107 52-53 0.1928600788587147 1107 54-55 0.25983102369823996 1107 56-57 0.0676856117935003 1107 58-59 0.11587030007462573 1107 60-61 0.30920912803723155 1107 62-63 0.23566399885284994 1107 64-65 0.24703114107401802 1107 66-67 0.1656541343475979 1107 68-69 0.3393037855506762 1107 70-71 0.5379036804660018 1107 72-73 0.39036421988585346 1107 74-75 0.3679656077024944 1107 76-77 0.23383627821843334 1107 78-79 0.23853769926714818 1107 80-81 0.38684552682932605 1107 82-83 0.6239789521036556 1107 84-85 0.4096888680204742 1107 86-87 0.3771302549286304 1107 88-89 0.5682136598194916 1107 90-91 0.24444924834315174 1107 92-93 0.43223513892419163 1107 94-95 0.4454065484873375 1107 96-97 0.2464333198200208 1107 98-99 0.11632849626348474 1107 100-101 0.38311414129669785 1108 1 -0.09857600328848548 1108 2 -0.01724460902656233 1108 3 -0.018156274296543984 1108 4 0.02231374511822537 1108 5 -0.023565594014336 1108 6 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-0.6748031205167635 2316 70-71 -0.6361940867991649 2316 72-73 -0.6145675974814537 2316 74-75 -0.5832367719699434 2316 76-77 -0.5508177016403621 2316 78-79 -0.7990684721122747 2316 80-81 -0.7598595765645726 2316 82-83 -0.7726840411228757 2316 84-85 -0.6984549695597124 2316 86-87 -0.7432542019398127 2316 88-89 -0.6165827778395432 2316 90-91 -0.7915894392531619 2316 92-93 -0.7947171601117553 2316 94-95 -0.7660671281782534 2316 96-97 -0.5742373965904015 2316 98-99 -0.8856152911428872 2316 100-101 -0.8275536184423977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 14.0 5 47.0 6 82.0 7 156.0 8 350.0 9 701.0 10 1118.0 11 1903.0 12 2936.0 13 4427.0 14 5592.0 15 7636.0 16 9901.0 17 13662.0 18 12594.0 19 8121.0 20 7187.0 21 7348.0 22 7953.0 23 9018.0 24 10492.0 25 12761.0 26 15807.0 27 20030.0 28 24934.0 29 31359.0 30 39011.0 31 48461.0 32 61135.0 33 78498.0 34 110325.0 35 165036.0 36 280699.0 37 516330.0 38 597946.0 39 112689.0 40 123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.04064828291853 18.15635312936836 13.014566010001296 23.788432577711806 2 16.15848234742341 19.31747323856091 39.70401138562888 24.820033028386803 3 20.163152154970295 23.664922735839394 28.256126066076533 27.915799043113783 4 12.825078108577662 15.274022317763123 35.87981969000911 36.02107988365011 5 14.136799156301597 37.36244535992827 33.39090041807628 15.10985506569385 6 34.32586772835642 34.776013884372354 16.63353377758055 14.264584609690672 7 30.598260500865077 31.515486069813527 19.411800638523026 18.474452790798367 8 27.478004732328777 34.988735117149396 18.58243037601712 18.95082977450471 9 27.05539196222761 16.06274378050906 19.413013365145698 37.468850892117636 10-11 26.14419993702785 26.00489314272804 26.302057015398532 21.548849904845575 12-13 26.701075195406364 22.78603081406369 27.297153323817163 23.21574066671278 14-15 23.10244216192326 24.341983517697724 25.4814075534004 27.074166766978625 16-17 23.33990829489455 28.153382032815223 25.677272319965795 22.82943735232444 18-19 22.992084039260003 26.276327396662218 28.050613819334274 22.680974744743505 20-21 23.61027611333432 25.53379658024337 28.16139515757933 22.69453214884298 22-23 23.167647852776717 25.607497708170236 28.03638810323632 23.18846633581673 24-25 23.12057021106676 25.8775695555046 28.148568037987122 22.85329219544152 26-27 23.044441145537277 26.32983077729165 27.91652527156282 22.709202805608253 28-29 22.630706495680005 26.185250323008656 28.301038456278 22.883004725033334 30-31 22.855131245620655 25.890287285075193 28.361248854632677 22.893332614671483 32-33 22.67822998260382 26.02408604932466 28.29088944196131 23.006794526110212 34-35 23.284239414825645 26.02765968437057 27.849300086015422 22.838800814788364 36-37 23.293204799254397 25.951636900584468 27.757072836601893 22.998085463559242 38-39 22.972061910171053 26.086906723763658 28.01880355930932 22.92222780675597 40-41 23.133752325812505 25.8184782645312 28.20228870056561 22.845480709090683 42-43 23.085832251387775 25.95795695440018 27.792626689817045 23.163584104395003 44-45 23.056872399812782 26.215344686791703 27.64885580532924 23.078927108066274 46-47 22.955929355797284 26.442880662688083 27.227733891562988 23.37345608995164 48-49 23.466625936915307 26.394136712704764 27.08425892442011 23.054978425959828 50-51 23.19608918986479 26.464380853901837 26.94123880202467 23.3982911542087 52-53 23.500204944496538 26.448193961717493 26.991762915714467 23.0598381780715 54-55 23.186775477131253 26.27396323806504 27.08110344195665 23.458157842847065 56-57 23.175174408119602 26.865486944685063 26.846239897265235 23.113098749930096 58-59 23.474009808433042 26.302249489579243 26.990640957316003 23.233099744671716 60-61 23.348053237798812 26.43164227735031 27.27692697084309 22.94337751400779 62-63 23.273324326218074 26.39739793782575 27.172667118220183 23.156610617735993 64-65 23.0601469160498 26.275690040327916 27.14030237963217 23.523860663990117 66-67 23.06877776919387 26.761754914237766 26.779069955461495 23.390397361106867 68-69 23.005603695312768 26.457944437357728 27.27058356405083 23.265868303278676 70-71 23.010746361304204 26.4580890561658 27.189410260550584 23.341754321979412 72-73 23.15746519367639 26.444159524388628 27.0556746215957 23.342700660339286 74-75 22.984672932121445 26.56169993729754 26.89219039987639 23.56143673070462 76-77 23.129664391110616 26.660594116797277 26.861627320353858 23.348114171738253 78-79 23.1943263890934 26.626400581083367 26.85631158189874 23.322961447924495 80-81 22.888416719873643 27.207131254327393 26.76796316512743 23.136488860671538 82-83 23.23108101552829 27.012452456624814 26.486196476085926 23.27027005176097 84-85 23.208679224604673 26.571344257523666 26.40762513396222 23.81235138390944 86-87 23.068162578183127 26.536090323111583 26.919077188953306 23.476669909751987 88-89 23.28628617820211 26.64838602852647 26.624464129123137 23.440863664148278 90-91 23.142674480266038 26.885191541164446 26.778322983633164 23.19381099493636 92-93 23.0935890245578 26.650742274868065 26.78066008518536 23.475008615388774 94-95 23.09346119726455 26.61644189415292 26.71467553030218 23.57542137828035 96-97 23.10424129451001 26.525866618755124 26.726578554344044 23.643313532390824 98-99 22.875946460550836 26.421436980561204 26.68541508614436 24.0172014727436 100-101 22.89653301424919 26.555894522568558 26.72089390272605 23.826678560456205 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 178.0 1 127.0 2 166.5 3 306.5 4 261.0 5 230.0 6 512.5 7 1036.5 8 1607.5 9 1888.0 10 1626.5 11 1354.5 12 1493.5 13 1712.5 14 1873.0 15 1970.5 16 2057.0 17 2193.5 18 2319.0 19 2455.0 20 2670.0 21 3022.5 22 3557.0 23 4297.0 24 5304.0 25 6573.5 26 8515.0 27 10897.0 28 13084.5 29 15581.0 30 18638.0 31 22258.5 32 26907.5 33 31375.0 34 36057.5 35 40217.5 36 45169.0 37 50670.0 38 54139.0 39 56755.0 40 59273.5 41 63660.5 42 68267.5 43 72167.0 44 75228.5 45 78027.0 46 79996.0 47 82274.5 48 90869.5 49 94003.5 50 89992.5 51 87300.5 52 84671.5 53 83865.0 54 83454.5 55 79154.5 56 71523.5 57 64595.5 58 58825.5 59 52822.0 60 45865.5 61 38152.0 62 31656.0 63 25522.5 64 20661.5 65 17012.0 66 12419.0 67 9976.0 68 6865.5 69 3574.0 70 2714.5 71 2325.5 72 2067.0 73 1902.0 74 1597.0 75 1348.5 76 886.0 77 406.5 78 205.0 79 121.0 80 79.0 81 48.5 82 20.5 83 11.5 84 7.0 85 2.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2639252457343464 2 0.019044299556052222 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.144106058782207E-4 12-13 8.983160167949163E-5 14-15 0.0 16-17 5.614475104968227E-4 18-19 0.0 20-21 6.737370125961873E-5 22-23 4.4915800839745816E-5 24-25 0.0024479111457661466 26-27 0.0027623217516443677 28-29 0.0024479111457661466 30-31 0.0012576424235128828 32-33 0.0016618846310705948 34-35 0.0019313794361090699 36-37 2.2457900419872908E-5 38-39 4.4915800839745816E-5 40-41 6.961949130160601E-4 42-43 0.003346227162561063 44-45 0.004761074889013056 46-47 0.01360948765444298 48-49 0.014956961679635354 50-51 0.006669996424702253 52-53 0.00788272304737539 54-55 4.0424220755771236E-4 56-57 0.0032563955608815713 58-59 0.0017292583323302137 60-61 0.0016843425314904679 62-63 0.0 64-65 6.512791121763143E-4 66-67 0.0 68-69 0.0 70-71 2.9195270545834777E-4 72-73 0.001459763527291739 74-75 0.0 76-77 0.19614730226716998 78-79 0.1018016626032839 80-81 8.758581163750433E-4 82-83 0.0 84-85 6.288212117564414E-4 86-87 0.12048663575261814 88-89 0.580985883862112 90-91 0.30717916194302164 92-93 0.2929857688776619 94-95 0.002829695452903986 96-97 0.002829695452903986 98-99 0.0030093586562629695 100-101 7.18652813435933E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2226388.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.29726237869794 #Duplication Level Percentage of deduplicated Percentage of total 1 70.57506134391811 31.96857071088161 2 15.157381404382336 13.731757648966076 3 5.643538607828716 7.669105471893871 4 2.821000604168282 5.111344181499043 5 1.5722963236643337 3.5610359555042748 6 0.9418304780873745 2.559740536930699 7 0.6212401773457968 1.9698335519394978 8 0.4209309108694651 1.525361433036678 9 0.3253505134440398 1.3263738815266886 >10 1.6635538530083478 13.7976180112772 >50 0.15154710327235638 4.804147297800743 >100 0.09813540395138011 8.238842658421762 >500 0.005752805017704959 1.7388296018732485 >1k 0.0022812847484002326 1.7064809554635292 >5k 9.918629340870576E-5 0.290958102985146 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6476 0.2908747262381939 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4690 0.21065510593840786 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3108 0.13959830900993 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2632 0.11821838781021098 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 2576 0.11570310296318521 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 4.4915800839745816E-5 0.0 0.0 0.0 4 0.0 4.4915800839745816E-5 0.0 0.0 0.0 5 0.0 4.4915800839745816E-5 0.0 0.0 0.0 6 0.0 4.4915800839745816E-5 0.0 0.0 0.0 7 0.0 4.4915800839745816E-5 0.0 0.0 0.0 8 0.0 4.4915800839745816E-5 0.0 0.0 0.0 9 0.0 4.4915800839745816E-5 0.0 0.0 0.0 10-11 0.0 4.4915800839745816E-5 0.0 0.0 0.0 12-13 0.0 4.4915800839745816E-5 0.0 4.4915800839745816E-5 0.0 14-15 0.0 4.4915800839745816E-5 0.0 6.737370125961873E-5 0.0 16-17 0.0 4.4915800839745816E-5 0.0 1.1228950209936454E-4 0.0 18-19 0.0 4.4915800839745816E-5 0.0 2.2457900419872907E-4 0.0 20-21 0.0 4.4915800839745816E-5 0.0 4.7161590881733106E-4 0.0 22-23 0.0 4.4915800839745816E-5 0.0 0.001167810821833391 0.0 24-25 0.0 4.4915800839745816E-5 0.0 0.0019538373365289427 0.0 26-27 0.0 4.4915800839745816E-5 0.0 0.002358079544086655 0.0 28-29 0.0 4.4915800839745816E-5 0.0 0.002829695452903986 0.0 30-31 0.0 4.4915800839745816E-5 0.0 0.004109795776836742 0.0 32-33 0.0 4.4915800839745816E-5 0.0 0.007298817636458695 0.0 34-35 0.0 4.4915800839745816E-5 0.0 0.011139118608256962 0.0 36-37 0.0 4.4915800839745816E-5 0.0 0.01731504122372201 0.0 38-39 0.0 4.4915800839745816E-5 0.0 0.028319412429459734 0.0 40-41 0.0 4.4915800839745816E-5 0.0 0.043231458308255345 0.0 42-43 0.0 4.4915800839745816E-5 0.0 0.05877232539880739 0.0 44-45 0.0 4.4915800839745816E-5 0.0 0.0766712720334461 0.0 46-47 0.0 4.4915800839745816E-5 0.0 0.0968160087100721 0.0 48-49 0.0 4.4915800839745816E-5 0.0 0.12125020436689382 0.0 50-51 0.0 4.4915800839745816E-5 0.0 0.14855901127745927 0.0 52-53 0.0 4.4915800839745816E-5 0.0 0.1764068077981017 0.0 54-55 0.0 4.4915800839745816E-5 0.0 0.20569190994561595 0.0 56-57 2.2457900419872908E-5 4.4915800839745816E-5 0.0 0.2407711504014574 0.0 58-59 4.4915800839745816E-5 4.4915800839745816E-5 0.0 0.2819813976719242 0.0 60-61 4.4915800839745816E-5 4.4915800839745816E-5 0.0 0.32873874634609956 0.0 62-63 4.4915800839745816E-5 4.4915800839745816E-5 0.0 0.3739689577917236 0.0 64-65 4.4915800839745816E-5 4.4915800839745816E-5 0.0 0.42086105386841827 0.0 66-67 4.4915800839745816E-5 4.4915800839745816E-5 0.0 0.47476001487611325 0.0 68-69 4.4915800839745816E-5 4.4915800839745816E-5 0.0 0.5397756365916453 0.0 70-71 4.4915800839745816E-5 4.4915800839745816E-5 0.0 0.6122472812465751 0.0 72-73 4.4915800839745816E-5 4.4915800839745816E-5 0.0 0.6899291587989156 0.0 74-75 4.4915800839745816E-5 4.4915800839745816E-5 0.0 0.7681051101604931 0.0 76-77 4.4915800839745816E-5 4.4915800839745816E-5 0.0 0.8535574212581096 0.0 78-79 4.4915800839745816E-5 4.4915800839745816E-5 0.0 0.9543933941433389 0.0 80-81 4.4915800839745816E-5 4.4915800839745816E-5 0.0 1.073981713879162 0.0 82-83 4.4915800839745816E-5 4.4915800839745816E-5 0.0 1.1967141396737675 0.0 84-85 4.4915800839745816E-5 4.4915800839745816E-5 0.0 1.3234440717431104 0.0 86-87 4.4915800839745816E-5 4.4915800839745816E-5 0.0 1.4561478053241395 0.0 88-89 4.4915800839745816E-5 4.4915800839745816E-5 0.0 1.6083000806687782 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5405 0.0 37.31463 1 GTATCAA 8305 0.0 33.620754 1 ACGACCG 65 0.0058858404 29.189383 5 TCAACGC 9915 0.0 27.746843 4 ATCAACG 9925 0.0 27.718885 3 GTACATG 10090 0.0 27.295834 1 CAACGCA 10110 0.0 27.258581 5 TACATGG 10195 0.0 26.712236 2 AACGCAG 10430 0.0 26.467745 6 TATCAAC 10485 0.0 26.290165 2 ACATGGG 10025 0.0 26.259525 3 GTACCTG 1700 0.0 23.22406 1 ACGCAGA 12055 0.0 22.742537 7 CATGGGA 5460 0.0 22.326405 4 CGCAGAG 12245 0.0 22.31218 8 TACCTGG 1700 0.0 21.768637 2 AGTACAT 7295 0.0 20.876867 2 CATGGGG 4605 0.0 20.600542 4 GCAGAGT 13290 0.0 20.272236 9 GAGTACT 6545 0.0 20.256758 12-13 >>END_MODULE