FastQCFastQC Report
Thu 26 May 2016
SRR936339_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936339_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415353
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84960.6002742778656632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61690.4358629967223724No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA42500.30027844643703727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.29978387017231745No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39300.27766924576413093No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA36360.2568970426458982No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21560.15232948953370642No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18950.1338888602348672No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.1292963663481831No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG17280.12208968363369421No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG16680.11785045850752428No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16300.11516561592761665No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG16080.11361123338135433No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA15560.10993723827200706No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG15440.10908939324677308No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14340.10131748051546152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG801.1056258E-535.4926572
GTACATG106200.029.1064111
TACATGG108750.028.1112692
ACATGGG107800.027.3493183
TATCACG700.00854595127.0420232
CATGGGG39200.024.8690014
ATTATCG1151.3150756E-424.689676
ACTTGAC7350.024.4657298
ATGGGAG19700.023.3014375
CATGGGA66300.023.1264364
ACTGCGC1050.002244954222.5342228
ATGGGAT22600.021.7772035
GTCCAAG11750.021.754841
GTCGGGA8050.021.7511922
CATGGGT10350.021.4899164
TGCACCG7750.021.3719225
GCACCGT7800.021.234176
GTATTAG3600.021.0386311
TACCTGG10650.020.8845672
GAGTACT68250.020.87163212-13
Produced by FastQC (version 0.11.5)