##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936339_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1415353 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.771871045597813 33.0 31.0 34.0 30.0 34.0 2 31.846112595232427 33.0 31.0 34.0 30.0 34.0 3 31.90988396534292 34.0 31.0 34.0 30.0 34.0 4 35.42046118530148 37.0 35.0 37.0 33.0 37.0 5 35.42122636543675 37.0 35.0 37.0 33.0 37.0 6 35.532412055508416 37.0 35.0 37.0 33.0 37.0 7 35.47655673178352 37.0 35.0 37.0 33.0 37.0 8 35.486054715678705 37.0 35.0 37.0 33.0 37.0 9 37.136346904270525 39.0 37.0 39.0 34.0 39.0 10-11 37.05783080263369 39.0 37.0 39.0 33.0 39.0 12-13 36.965560535075 39.0 37.0 39.0 33.0 39.0 14-15 38.13499918394916 40.0 38.0 41.0 33.0 41.0 16-17 38.10728878237443 40.0 38.0 41.0 33.0 41.0 18-19 38.11481976581108 40.0 38.0 41.0 33.0 41.0 20-21 38.058012736045356 40.0 38.0 41.0 33.0 41.0 22-23 37.89535048853537 40.0 38.0 41.0 33.0 41.0 24-25 38.025985036948384 40.0 38.0 41.0 33.0 41.0 26-27 37.95268459529178 40.0 38.0 41.0 33.0 41.0 28-29 37.80434668948312 40.0 38.0 41.0 33.0 41.0 30-31 37.616197513977085 40.0 37.0 41.0 33.0 41.0 32-33 37.372987162919785 40.0 37.0 41.0 32.0 41.0 34-35 37.07163972521343 40.0 36.0 41.0 31.5 41.0 36-37 36.82490622480752 40.0 36.0 41.0 31.0 41.0 38-39 36.62397578554608 40.0 35.5 41.0 30.5 41.0 40-41 36.392817198253724 40.0 35.0 41.0 30.0 41.0 42-43 36.149164554708264 39.0 35.0 41.0 30.0 41.0 44-45 35.884196027422135 39.0 35.0 41.0 28.5 41.0 46-47 35.46665849438267 39.0 35.0 41.0 26.5 41.0 48-49 35.17692724005955 39.0 35.0 41.0 26.0 41.0 50-51 34.81752432078782 38.5 34.5 40.5 25.0 41.0 52-53 34.79471516999646 39.0 35.0 40.0 25.0 41.0 54-55 34.8475496925502 39.0 35.0 41.0 24.5 41.0 56-57 34.68215067195251 38.5 35.0 41.0 24.5 41.0 58-59 34.438714582157246 38.0 34.0 40.5 23.5 41.0 60-61 34.137198988520886 37.5 34.0 40.0 23.0 41.0 62-63 33.830809345795714 37.0 34.0 40.0 22.0 41.0 64-65 33.4913297248107 36.5 34.0 40.0 22.0 41.0 66-67 33.13008804164049 36.0 33.0 39.0 20.5 41.0 68-69 32.765140215903735 35.5 33.0 39.0 19.0 41.0 70-71 32.355430058790986 35.0 33.0 38.5 17.5 40.5 72-73 31.9641880859404 35.0 32.5 37.5 14.5 40.0 74-75 31.568895533481754 35.0 32.0 37.0 10.0 39.0 76-77 31.168719393677762 35.0 32.0 36.5 7.0 39.0 78-79 30.78567290280234 35.0 31.0 36.0 4.5 38.5 80-81 30.41468029530442 35.0 31.0 35.5 2.0 37.0 82-83 30.074792295632257 35.0 31.0 35.0 2.0 37.0 84-85 29.768626625301252 34.0 31.0 35.0 2.0 36.5 86-87 29.446582937260175 34.0 30.0 35.0 2.0 36.0 88-89 29.107384871477294 34.0 30.0 35.0 2.0 36.0 90-91 28.744419236755775 34.0 29.0 35.0 2.0 35.5 92-93 28.43893961435769 34.0 29.0 35.0 2.0 35.0 94-95 28.065411950234324 34.0 29.0 35.0 2.0 35.0 96-97 27.635720205489374 34.0 28.5 35.0 2.0 35.0 98-99 27.14077583472109 34.0 27.0 35.0 2.0 35.0 100-101 23.965475750572473 29.5 13.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25651505055120083 1101 2 0.21630623677684824 1101 3 0.24544267722746227 1101 4 0.09506115418138705 1101 5 0.3226313948530546 1101 6 0.18817288427104018 1101 7 0.28323921785823103 1101 8 0.32096520532415695 1101 9 0.29196200526332206 1101 10-11 0.49576401374039136 1101 12-13 0.3479523041970438 1101 14-15 0.4982840751998481 1101 16-17 0.45823065522451856 1101 18-19 0.4366357256820166 1101 20-21 0.5581058533973646 1101 22-23 0.555146571569118 1101 24-25 0.5203107512029987 1101 26-27 0.5293289261377723 1101 28-29 0.40879871719808847 1101 30-31 0.5681046826840728 1101 32-33 0.5901552535380574 1101 34-35 0.5126415490416107 1101 36-37 0.3783885089662462 1101 38-39 0.35464228662703334 1101 40-41 0.33523567978623703 1101 42-43 0.3744765569512154 1101 44-45 0.37605488410751775 1101 46-47 0.2900971179295837 1101 48-49 0.4063413971140477 1101 50-51 0.5947781110739996 1101 52-53 0.5360858587072599 1101 54-55 0.5547224728149516 1101 56-57 0.5526746765728632 1101 58-59 0.7916920478878424 1101 60-61 0.6050911005753434 1101 62-63 0.6048701943479742 1101 64-65 0.5936748315172835 1101 66-67 0.6706064353627639 1101 68-69 0.6051803761543582 1101 70-71 0.6793611311809755 1101 72-73 0.6330861681626416 1101 74-75 0.5097733399151814 1101 76-77 0.5542637432135997 1101 78-79 0.30152942253956994 1101 80-81 0.3068424771408367 1101 82-83 0.5061762975875332 1101 84-85 0.48425375343737187 1101 86-87 0.46429398629630825 1101 88-89 0.49748736637119606 1101 90-91 0.46950305557531635 1101 92-93 0.39721434017432955 1101 94-95 0.6883763497863562 1101 96-97 0.7483897463168283 1101 98-99 0.8792122838458667 1101 100-101 1.2474966880553815 1102 1 0.28421216682092876 1102 2 0.3374309975817731 1102 3 0.28608143268780495 1102 4 0.25814128941377845 1102 5 0.21179132500530073 1102 6 0.21814967607230074 1102 7 0.043357936721029944 1102 8 0.09079538379297247 1102 9 0.11283208956041335 1102 10-11 0.18274831473287634 1102 12-13 0.24923551953677503 1102 14-15 0.3577914867604335 1102 16-17 0.3392638803273158 1102 18-19 0.3671118982260495 1102 20-21 0.35411324822126744 1102 22-23 0.5113791026575072 1102 24-25 0.45156448208830824 1102 26-27 0.4466228696905219 1102 28-29 0.5378493428710414 1102 30-31 0.4981687144391387 1102 32-33 0.32933987029593226 1102 34-35 0.4232864970742156 1102 36-37 0.4532171671864589 1102 38-39 0.2840431273663384 1102 40-41 0.11641960476127622 1102 42-43 0.08097478286294546 1102 44-45 0.10141430434886445 1102 46-47 0.3598012133956203 1102 48-49 0.18851418103790962 1102 50-51 0.04986348209833835 1102 52-53 0.2949496751170173 1102 54-55 0.10829345308240335 1102 56-57 0.06711320274066424 1102 58-59 0.08163344160713137 1102 60-61 0.251267884566154 1102 62-63 0.5082451462300099 1102 64-65 0.33142288308857815 1102 66-67 0.4355896010503386 1102 68-69 0.33464586232707205 1102 70-71 0.4362716373540181 1102 72-73 0.45518465516661166 1102 74-75 0.5250295227243704 1102 76-77 0.42418067348300426 1102 78-79 0.39743758278772034 1102 80-81 0.2773078126834143 1102 82-83 0.11175128629027142 1102 84-85 0.16723973088748423 1102 86-87 0.17236162480629602 1102 88-89 0.14745884427598455 1102 90-91 0.34787828640205376 1102 92-93 0.45950722373723707 1102 94-95 0.34481969332906104 1102 96-97 0.16878241676598194 1102 98-99 0.2504161952083983 1102 100-101 0.24915187614778844 1103 1 0.32487175352193987 1103 2 0.2761244288036515 1103 3 0.3463970458364152 1103 4 0.2792526523716603 1103 5 0.40592741071674254 1103 6 0.36384641324377753 1103 7 0.36803204085291696 1103 8 0.40034117506482403 1103 9 0.48514393237854136 1103 10-11 0.5288074348416743 1103 12-13 0.5692623596460109 1103 14-15 0.583512382794396 1103 16-17 0.5523891360132112 1103 18-19 0.5098419878957827 1103 20-21 0.6068366485949852 1103 22-23 0.7426843169913937 1103 24-25 0.7488490978131281 1103 26-27 0.714325317641169 1103 28-29 0.5815101292226643 1103 30-31 0.5494586152606544 1103 32-33 0.6493743118779278 1103 34-35 0.534479458386862 1103 36-37 0.4943722317022363 1103 38-39 0.46761938534097425 1103 40-41 0.5171374602061363 1103 42-43 0.5106825822504106 1103 44-45 0.5468716642329952 1103 46-47 0.6285754690041259 1103 48-49 0.5909789931295037 1103 50-51 0.6928326726382394 1103 52-53 0.6843019467134894 1103 54-55 0.6227977541338916 1103 56-57 0.7209524993142011 1103 58-59 0.800250415833986 1103 60-61 0.7988779815333018 1103 62-63 0.6948018725659324 1103 64-65 0.5875828401886309 1103 66-67 0.6418914983779374 1103 68-69 0.5631516615597363 1103 70-71 0.6103847527968966 1103 72-73 0.7587522336414523 1103 74-75 0.7620918161025223 1103 76-77 0.7845430011368855 1103 78-79 0.7810825304343005 1103 80-81 0.7304053437250637 1103 82-83 0.8555623053851704 1103 84-85 0.7522774065506077 1103 86-87 0.8551369639701925 1103 88-89 0.7435615000923583 1103 90-91 0.9641296823088688 1103 92-93 0.8850618548308553 1103 94-95 0.8383100684824747 1103 96-97 0.8516779974079363 1103 98-99 0.8355754944355382 1103 100-101 1.0580625989234598 1104 1 0.3279696404008092 1104 2 0.20876423036964908 1104 3 0.258650028031834 1104 4 0.22749691870266986 1104 5 0.2257554733673075 1104 6 0.2410336430215665 1104 7 0.3326595407801918 1104 8 0.1968286499763039 1104 9 0.21538845785842398 1104 10-11 0.1808240362363165 1104 12-13 0.18308491141629446 1104 14-15 0.36834741337661114 1104 16-17 0.3570166623842539 1104 18-19 0.2722196524354885 1104 20-21 0.14305733237781482 1104 22-23 0.23308655307602777 1104 24-25 0.24175820266530224 1104 26-27 0.22148430547967735 1104 28-29 0.35582054198336976 1104 30-31 0.3335459484335601 1104 32-33 0.3839939580074869 1104 34-35 0.30897034983257043 1104 36-37 0.2937235661867561 1104 38-39 0.3447656272855468 1104 40-41 0.3738783721457608 1104 42-43 0.4391363360748386 1104 44-45 0.34464364495019595 1104 46-47 0.47390325267299005 1104 48-49 0.4017778327771637 1104 50-51 0.323666497384842 1104 52-53 0.2284597260720176 1104 54-55 0.33851539820611976 1104 56-57 0.34684795125134116 1104 58-59 0.2603381826846132 1104 60-61 0.41682047163671854 1104 62-63 0.3936206296561622 1104 64-65 0.3481973668056 1104 66-67 0.47999815136436297 1104 68-69 0.3707587389685685 1104 70-71 0.3263985670188845 1104 72-73 0.6350575639108271 1104 74-75 0.4864645039259621 1104 76-77 0.6747246962350069 1104 78-79 0.641754968893057 1104 80-81 0.7070561617628819 1104 82-83 0.8033927268194283 1104 84-85 0.5945244917867889 1104 86-87 0.5397197210498454 1104 88-89 0.6039780951405014 1104 90-91 0.7726913795753774 1104 92-93 0.6279246830971204 1104 94-95 0.6261656379356957 1104 96-97 0.8809122389082482 1104 98-99 0.9259853992115445 1104 100-101 1.1339086010230979 1105 1 0.08823622268025844 1105 2 0.13591734360992902 1105 3 0.19847774592942002 1105 4 -0.011718610579315225 1105 5 0.09188798417942223 1105 6 0.1282909185598271 1105 7 0.24093800335687376 1105 8 0.2706337892088655 1105 9 0.19388179012420892 1105 10-11 0.26887873288078623 1105 12-13 0.25720332549573754 1105 14-15 0.17341039242341338 1105 16-17 0.17697417646891012 1105 18-19 0.20395337771187627 1105 20-21 0.2906726944104321 1105 22-23 0.45673365690407763 1105 24-25 0.2922990307021891 1105 26-27 0.065760017846344 1105 28-29 0.3294997358312344 1105 30-31 0.35582658931891586 1105 32-33 0.2981388574735817 1105 34-35 0.0919385559305681 1105 36-37 0.2505726566729862 1105 38-39 0.45787786355959526 1105 40-41 0.23703699597320593 1105 42-43 0.3515331237685402 1105 44-45 0.3483282782152841 1105 46-47 0.33776595059850223 1105 48-49 0.352249587840717 1105 50-51 0.12880922489672741 1105 52-53 0.33566549236262944 1105 54-55 0.5998965526588833 1105 56-57 0.683737557770506 1105 58-59 0.5621441835818644 1105 60-61 0.5226349290485004 1105 62-63 0.39704059968853755 1105 64-65 0.3719271421500494 1105 66-67 0.2909479015705614 1105 68-69 0.46850207981286474 1105 70-71 0.35515186021745393 1105 72-73 0.3917427728899341 1105 74-75 0.31466629359590215 1105 76-77 0.39393416336275067 1105 78-79 0.2400343083851304 1105 80-81 0.14516531612413885 1105 82-83 0.2680187224043493 1105 84-85 0.34057297122820884 1105 86-87 0.28866615168647414 1105 88-89 0.34223219471995847 1105 90-91 0.3713675550550448 1105 92-93 0.24546979022695226 1105 94-95 0.320649585935886 1105 96-97 0.309104941701289 1105 98-99 0.3374283825539628 1105 100-101 0.6143448092409365 1106 1 0.1535993360352066 1106 2 0.17443689239383176 1106 3 0.2949804298041947 1106 4 0.19386168138049698 1106 5 0.2113000906673861 1106 6 0.12055991416146128 1106 7 0.15224098467060543 1106 8 0.06535454882219227 1106 9 0.17198691131252275 1106 10-11 0.27134976419315393 1106 12-13 0.18401076387800686 1106 14-15 0.17144843932122455 1106 16-17 0.2645244897130752 1106 18-19 0.1749840879828568 1106 20-21 0.2133653598909504 1106 22-23 0.13852936932757842 1106 24-25 0.15580345164453746 1106 26-27 0.32110834956905876 1106 28-29 0.31686821392521125 1106 30-31 0.26967616588987653 1106 32-33 0.35616955871301315 1106 34-35 0.18355457067005432 1106 36-37 0.27607774594982715 1106 38-39 0.4321238046317646 1106 40-41 0.46498481771455147 1106 42-43 0.5176262871883637 1106 44-45 0.4163325170214307 1106 46-47 0.4542674904772994 1106 48-49 0.5786403708457541 1106 50-51 0.5345487731387308 1106 52-53 0.6337453854291084 1106 54-55 0.4359917331242542 1106 56-57 0.5512504804092799 1106 58-59 0.5842025333977716 1106 60-61 0.6391605530531521 1106 62-63 0.5448861241053251 1106 64-65 0.5249353999946962 1106 66-67 0.7614939188087746 1106 68-69 0.6871326564169422 1106 70-71 0.6723732144076351 1106 72-73 0.7054376089523018 1106 74-75 0.7255520174292478 1106 76-77 0.7328748832974874 1106 78-79 0.7082613253554051 1106 80-81 0.6493240425555662 1106 82-83 0.7119707468256529 1106 84-85 0.5744120712044243 1106 86-87 0.5177082958962025 1106 88-89 0.5197969283344754 1106 90-91 0.6064123665817078 1106 92-93 0.3941829452775423 1106 94-95 0.7320118744095225 1106 96-97 0.620487701681899 1106 98-99 0.3943379677350016 1106 100-101 0.5654037581494755 1107 1 0.05198457737109052 1107 2 0.0438121799867659 1107 3 0.10630008784105272 1107 4 0.008440524227609103 1107 5 0.0035399250138539173 1107 6 0.16024773959557592 1107 7 0.1776181855134027 1107 8 0.1032617198764143 1107 9 0.1005688056720686 1107 10-11 0.09196260960741398 1107 12-13 -0.008624737805163818 1107 14-15 0.2166728829045752 1107 16-17 0.04887519567805754 1107 18-19 0.16526465759850595 1107 20-21 0.23662617944000885 1107 22-23 0.09807170300803847 1107 24-25 0.21020755907715483 1107 26-27 0.2523339798516844 1107 28-29 0.23161991526448844 1107 30-31 0.07717204876993122 1107 32-33 0.16226531807946287 1107 34-35 0.20564927653815346 1107 36-37 0.2570527162823524 1107 38-39 0.07990086258627827 1107 40-41 0.2180472284093753 1107 42-43 -0.07172581133189482 1107 44-45 0.2595011251927062 1107 46-47 0.30029356153395526 1107 48-49 0.21858460808873303 1107 50-51 0.21880290842245387 1107 52-53 0.37095264609124 1107 54-55 0.49961243739394234 1107 56-57 0.5213943979700062 1107 58-59 0.5382657675165845 1107 60-61 0.5426370975785915 1107 62-63 0.7008374815439922 1107 64-65 0.5988159066426348 1107 66-67 0.4537146812025199 1107 68-69 0.3837551044080314 1107 70-71 0.5353557650845389 1107 72-73 0.35421427941317774 1107 74-75 0.38315748835512053 1107 76-77 0.4746551744020806 1107 78-79 0.40249251374997286 1107 80-81 0.48655309973509375 1107 82-83 0.6102364100452426 1107 84-85 0.7288019503548462 1107 86-87 0.4591274308166433 1107 88-89 0.4737396761487709 1107 90-91 0.4938781581585623 1107 92-93 0.2655977208945046 1107 94-95 0.38934155884438937 1107 96-97 0.588413823597083 1107 98-99 0.4472726266195046 1107 100-101 0.49817936300282994 1108 1 -0.09687754381991098 1108 2 -0.03466916934678821 1108 3 -0.053088893190039954 1108 4 0.04720122704809171 1108 5 -0.19275955883493623 1108 6 -0.0031958856349731946 1108 7 0.027663714603242795 1108 8 -0.0978473548145189 1108 9 -0.048410622612649945 1108 10-11 -0.034985152730740765 1108 12-13 -0.04163536048515226 1108 14-15 -0.11417638561020027 1108 16-17 0.08088899842559982 1108 18-19 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68-69 -2.1254842042932793 2316 70-71 -1.994341938173136 2316 72-73 -2.0649493525992995 2316 74-75 -2.010756872476424 2316 76-77 -2.2225569044141373 2316 78-79 -2.3184375631316527 2316 80-81 -1.9832151341296402 2316 82-83 -2.125327575415195 2316 84-85 -2.372885671603111 2316 86-87 -2.303376083580673 2316 88-89 -2.2623751074145595 2316 90-91 -2.3024499660569724 2316 92-93 -2.207246656571847 2316 94-95 -2.3949765309300552 2316 96-97 -2.8399943468746116 2316 98-99 -2.4085889697201672 2316 100-101 -2.1977614662610527 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9789.0 3 2233.0 4 1915.0 5 1734.0 6 1648.0 7 1802.0 8 2270.0 9 2791.0 10 3333.0 11 4143.0 12 5817.0 13 8614.0 14 8900.0 15 9051.0 16 11188.0 17 15020.0 18 13246.0 19 7818.0 20 7308.0 21 7695.0 22 7744.0 23 8477.0 24 9399.0 25 10967.0 26 12865.0 27 14929.0 28 18423.0 29 22259.0 30 26934.0 31 33306.0 32 42442.0 33 55989.0 34 79313.0 35 123771.0 36 194705.0 37 294082.0 38 280725.0 39 52677.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.69244390093908 18.0707365950724 12.84766573477488 22.38915376921364 2 15.943543617388872 20.082660804518998 39.44131528051889 24.53248029757324 3 20.35148289575213 25.384538200853022 28.55489385275904 25.70908505063581 4 12.372930337526418 15.091004029628333 36.754831420502285 35.78123421234297 5 13.623391168251208 38.64587859966719 32.47948220613538 15.251248025946222 6 32.5058663179025 35.11983115744342 17.078563085969378 15.295739438684702 7 29.753485673991182 33.30900385997811 19.424599711861784 17.512910754168924 8 27.820197026931158 33.37707155969526 19.091276520274327 19.711454893099255 9 28.45132049232804 15.69143711567787 19.69594828548695 36.161294106507135 10-11 26.27530598935794 25.840070881146193 25.9693696227091 21.915253506786772 12-13 27.530591941166243 23.316047714807787 27.118082985100784 22.035277358925185 14-15 23.874482909197283 24.721047419553937 26.13351921339665 25.27095045785213 16-17 22.642600094339457 28.210959983464097 26.559454173814263 22.586985748382187 18-19 22.38278538078742 26.406972902979632 29.30647244102834 21.90376927520461 20-21 23.514060715384666 25.32105191200364 28.90055419012886 22.26433318248283 22-23 22.885014412989108 25.32372255411098 28.916423741526476 22.87483929137344 24-25 22.71036217051437 25.63874251757251 29.237409858412917 22.413485453500208 26-27 22.95249868309998 25.48808324715926 29.508982653662187 22.05043541607858 28-29 22.090827697175996 25.89393542168538 29.82579556913588 22.189441312002735 30-31 22.406222352871342 25.770197773249027 29.507912908541535 22.315666965338096 32-33 21.991677348509793 25.966488926450033 29.52403732685875 22.517796398181424 34-35 22.74636955734707 25.606762326005494 29.2230707483774 22.423797368270034 36-37 23.172954756918767 25.36615098871506 29.10943403460281 22.351460219763368 38-39 22.901724541885283 25.403481742859373 29.298291077678932 22.39650263757641 40-41 22.50995315722704 25.850088214105522 29.428254006915815 22.211704621751622 42-43 22.364961896968268 26.223335422062306 28.61744485134226 22.79425782962717 44-45 22.400385189930095 26.20065597333781 28.357087540874055 23.041871295858034 46-47 22.739622220746575 26.476577977471592 27.784597716904546 22.999202084877286 48-49 23.300608507744403 26.256034915950643 27.54517641752829 22.89818015877666 50-51 22.84942875895361 26.53916147894747 27.626273962716613 22.985135799382302 52-53 23.062862849212404 26.806131450317267 27.335978306109432 22.795027394360893 54-55 22.672758403479207 26.284764905687442 27.473853751446057 23.56862293938729 56-57 23.015251373916083 27.009567526226725 26.82678112002477 23.148399979832423 58-59 23.350047080644956 26.215149315945922 27.105988970489026 23.328814632920096 60-61 23.094172712164028 26.18563999546965 27.933091406925968 22.787095885440355 62-63 23.38381037522625 26.24620374821504 27.565669741538894 22.804316135019814 64-65 22.89629243235201 26.65169118647065 27.608100252862144 22.843916128315197 66-67 23.046232403791063 26.652483437713663 27.13868443155734 23.162599726937934 68-69 22.68626920430117 26.736598623802717 27.593437318680785 22.98369485321533 70-71 22.86037563292465 26.729958539929548 27.40127937583387 23.00838645131193 72-73 22.9662892145654 26.75165138253135 26.9812684719794 23.30079093092385 74-75 22.674051936467173 27.470867056621035 26.753729329246294 23.1013516776655 76-77 22.837262742441382 26.83012042802758 27.05668191278821 23.275934916742827 78-79 22.985907771904092 26.580631325853883 27.32864740386797 23.104813498374057 80-81 22.668439637617993 27.408592281185147 27.09133936829921 22.831628712897654 82-83 22.851892947494562 27.173476543429054 26.732200079889928 23.242430429186452 84-85 22.749282311940874 26.712731262438595 26.55262956999079 23.985356855629746 86-87 22.71324548436888 26.69183704846796 26.9625494403419 23.63236802682126 88-89 22.98297755827593 26.645964960752817 26.84423398657112 23.526823494400134 90-91 22.864594174677272 26.651821203697324 26.97239937397566 23.511185247649742 92-93 22.68420205834499 26.862691404880657 26.844968484100697 23.60813805267366 94-95 22.581358727588736 26.81774686344374 26.908750046602293 23.69214436236523 96-97 22.330944179561378 26.762403200935974 26.81277279090834 24.093879828594307 98-99 22.56491564701619 26.695871032272102 26.518437119217204 24.220776201494505 100-101 22.724829956361205 26.696088126655443 26.43572488887974 24.143357028103612 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 912.0 1 626.5 2 692.5 3 1065.0 4 772.0 5 702.0 6 1601.0 7 2912.5 8 4092.5 9 4216.5 10 3169.0 11 2418.0 12 2451.0 13 2622.0 14 2716.0 15 2818.0 16 2945.5 17 2958.5 18 2927.5 19 3042.0 20 3174.5 21 3389.0 22 3666.5 23 4226.0 24 4880.5 25 5601.5 26 7076.5 27 8676.5 28 9869.0 29 11096.0 30 13284.0 31 16518.5 32 19244.5 33 21437.5 34 23801.0 35 25422.5 36 28442.0 37 32039.5 38 32934.0 39 32446.5 40 32337.0 41 33709.0 42 35078.0 43 37010.5 44 39824.0 45 42907.0 46 44554.0 47 47929.5 48 64808.0 49 65840.5 50 53108.5 51 49455.5 52 46795.5 53 47741.0 54 48623.5 55 47922.0 56 43718.5 57 38769.5 58 37136.0 59 35380.5 60 32549.5 61 28843.0 62 24953.0 63 20053.5 64 15398.0 65 12910.0 66 9488.5 67 7433.5 68 5227.0 69 2740.0 70 2081.0 71 1700.5 72 1441.0 73 1456.0 74 1198.0 75 946.5 76 746.5 77 409.0 78 194.5 79 106.0 80 70.0 81 49.0 82 35.5 83 26.0 84 21.5 85 20.0 86 15.0 87 10.5 88 15.0 89 17.0 90 14.5 91 14.5 92 10.5 93 9.5 94 12.0 95 10.5 96 11.5 97 12.5 98 10.5 99 9.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04719670640469197 2 0.012152445361687155 3 0.010527409063322012 4 0.006076222680843578 5 0.008337142748134211 6 0.0049457626471982605 7 0.0042392251261699375 8 0.008125181491825714 9 0.014060096668463626 10-11 0.0018369975546736396 12-13 0.007842566483414385 14-15 0.007312663342643143 16-17 0.017698764901759492 18-19 0.011339927212504583 20-21 0.0031440919685760374 22-23 0.009644237162036608 24-25 0.020312953729564286 26-27 0.0068887408300261485 28-29 0.016532977992062756 30-31 0.013918789164257962 32-33 0.01264702162640698 34-35 0.27456048067160627 36-37 0.4863451025998461 38-39 0.48489670068173807 40-41 0.5038672331213485 42-43 0.49461159159587753 44-45 0.5098728020500893 46-47 0.5163729472435499 48-49 0.5228730924370104 50-51 0.5327999446074584 52-53 0.5227671118088562 54-55 0.5098374751740379 56-57 0.5053156350394566 58-59 0.5038319062452971 60-61 0.49976931549938425 62-63 0.49853287483758474 64-65 0.5139000659199507 66-67 0.4862391219716919 68-69 0.5136527777875908 70-71 0.4952828022408544 72-73 0.5043971362621197 74-75 0.5175387341532466 76-77 0.5034786374847829 78-79 0.5012530442935437 80-81 0.506622729453359 82-83 0.5062694606928448 84-85 0.5076825357349015 86-87 0.5098728020500893 88-89 0.5240742062227586 90-91 0.5468600412759219 92-93 0.5349548840465947 94-95 0.5059161919323306 96-97 0.47797263297566045 98-99 0.47051866212881166 100-101 0.476488904181501 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1415353.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.847827275081436 #Duplication Level Percentage of deduplicated Percentage of total 1 65.03800305954314 22.013950938756345 2 13.742465491596912 9.303051965866787 3 6.615338947748023 6.717445502084822 4 3.7895918205144397 5.130777975353366 5 2.3693168143596988 4.009811314619665 6 1.6420846994920069 3.3348599567675676 7 1.1778577824234564 2.7907548741855726 8 0.8481853587474781 2.2967385216110103 9 0.6766305304960901 2.061222599275055 >10 3.7195509663845114 22.57041635930645 >50 0.23399547961837716 5.503863561048644 >100 0.13441674359123357 8.286573545516786 >500 0.007118639774642411 1.751001380940895 >1k 0.005024922193865231 3.190255349110259 >5k 4.18743516155436E-4 1.039276155556833 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8496 0.6002742778656632 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6169 0.4358629967223724 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4250 0.30027844643703727 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4243 0.29978387017231745 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3930 0.27766924576413093 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3636 0.2568970426458982 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2156 0.15232948953370642 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1895 0.1338888602348672 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1830 0.1292963663481831 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 1728 0.12208968363369421 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1668 0.11785045850752428 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1630 0.11516561592761665 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 1608 0.11361123338135433 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1556 0.10993723827200706 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1544 0.10908939324677308 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1434 0.10131748051546152 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.06537521028323E-5 0.0 12-13 0.0 0.0 0.0 3.8859563656557764E-4 0.0 14-15 0.0 0.0 0.0 5.652300168226584E-4 0.0 16-17 0.0 0.0 0.0 0.0020136319349307206 0.0 18-19 0.0 0.0 0.0 0.0027201694559590437 0.0 20-21 0.0 0.0 0.0 0.0042392251261699375 0.0 22-23 0.0 0.0 0.0 0.007842566483414384 0.0 24-25 0.0 0.0 0.0 0.011693195973018745 0.0 26-27 0.0 0.0 0.0 0.01338888602348672 0.0 28-29 0.0 0.0 0.0 0.01568513296682877 0.0 30-31 0.0 0.0 0.0 0.021231452506901106 0.0 32-33 0.0 0.0 0.0 0.028756077105852745 0.0 34-35 0.0 0.0 0.0 0.041332444980156896 0.0 36-37 0.0 0.0 0.0 0.06242258998285233 0.0 38-39 0.0 0.0 0.0 0.09322762589968722 7.06537521028323E-5 40-41 0.0 0.0 0.0 0.12767133004981795 7.06537521028323E-5 42-43 0.0 0.0 0.0 0.16070195915789207 7.06537521028323E-5 44-45 0.0 0.0 0.0 0.19903161967367858 7.06537521028323E-5 46-47 0.0 0.0 0.0 0.2495843793032551 7.06537521028323E-5 48-49 0.0 0.0 0.0 0.30748512915152615 7.06537521028323E-5 50-51 0.0 0.0 0.0 0.3644673802224604 7.06537521028323E-5 52-53 0.0 0.0 0.0 0.42084907440052055 7.06537521028323E-5 54-55 0.0 0.0 0.0 0.48069280243161955 7.06537521028323E-5 56-57 0.0 0.0 0.0 0.5515585157907603 7.06537521028323E-5 58-59 0.0 0.0 0.0 0.6357777882973363 7.06537521028323E-5 60-61 0.0 0.0 0.0 0.7344104262328903 7.06537521028323E-5 62-63 0.0 0.0 0.0 0.8284858971578115 7.06537521028323E-5 64-65 0.0 0.0 0.0 0.9174036441792259 7.06537521028323E-5 66-67 0.0 0.0 0.0 1.0213353135224923 7.06537521028323E-5 68-69 0.0 0.0 0.0 1.1468870310092254 7.06537521028323E-5 70-71 0.0 0.0 0.0 1.2880532277106842 7.06537521028323E-5 72-73 0.0 0.0 0.0 1.4355076083492952 7.06537521028323E-5 74-75 0.0 0.0 0.0 1.5761792287860343 7.06537521028323E-5 76-77 0.0 0.0 0.0 1.7365632460594635 7.06537521028323E-5 78-79 0.0 0.0 0.0 1.9328040425250803 7.06537521028323E-5 80-81 0.0 0.0 0.0 2.1508061946383696 7.06537521028323E-5 82-83 0.0 0.0 0.0 2.370009460537407 7.06537521028323E-5 84-85 0.0 0.0 0.0 2.5916502808839916 7.06537521028323E-5 86-87 0.0 0.0 0.0 2.826326718493549 7.06537521028323E-5 88-89 0.0 0.0 0.0 3.087462986265617 7.06537521028323E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 80 1.1056258E-5 35.492657 2 GTACATG 10620 0.0 29.106411 1 TACATGG 10875 0.0 28.111269 2 ACATGGG 10780 0.0 27.349318 3 TATCACG 70 0.008545951 27.042023 2 CATGGGG 3920 0.0 24.869001 4 ATTATCG 115 1.3150756E-4 24.68967 6 ACTTGAC 735 0.0 24.465729 8 ATGGGAG 1970 0.0 23.301437 5 CATGGGA 6630 0.0 23.126436 4 ACTGCGC 105 0.0022449542 22.534222 8 ATGGGAT 2260 0.0 21.777203 5 GTCCAAG 1175 0.0 21.75484 1 GTCGGGA 805 0.0 21.751192 2 CATGGGT 1035 0.0 21.489916 4 TGCACCG 775 0.0 21.371922 5 GCACCGT 780 0.0 21.23417 6 GTATTAG 360 0.0 21.038631 1 TACCTGG 1065 0.0 20.884567 2 GAGTACT 6825 0.0 20.871632 12-13 >>END_MODULE