FastQCFastQC Report
Thu 26 May 2016
SRR936339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936339_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415353
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86840.6135571832609957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66060.46673868639131016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46200.3264203347150852No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42740.3019741364875052No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA42160.29787621886554094No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA37170.26261999656622764No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT22760.1608079397860463No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG18800.13282905395332473No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG18650.13176924767178225No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18070.12767133004981795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18060.12760067629771513No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG17810.12583433249514434No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16570.11707326723439312No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15600.11021985328041838No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG15390.1087361244862589No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA15260.10781762570892209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCT953.5254678E-529.9573613
TATAACG1354.2712418E-728.1141032
GTACATG101700.027.8809341
TACATGG104550.026.7275562
ACATGGG100950.026.5941453
CATGGGG35050.026.3889754
TAGTCGA1201.7276683E-423.7162425
TACCTGG12000.023.3259182
CCGTAAG2052.6258931E-823.2074871
TATAGGG2252.8539944E-923.1941342
ATGGGAG21250.022.7675955
ATAGCGC1252.2780932E-422.7675938
ATGGGGG16200.022.252285
GTACCGT1503.0673495E-522.1351626
CATGGGA63050.022.0423774
GTATAGG3900.021.9578531
GAGTACT72650.021.74125112-13
AGTACTT76450.020.72263312-13
GTCCAAG11050.020.6662161
GTACTTT76650.020.39009314-15
Produced by FastQC (version 0.11.5)