##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936339_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1415353 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48280958884462 34.0 31.0 34.0 31.0 34.0 2 32.592237413563964 34.0 31.0 34.0 31.0 34.0 3 32.67700990494951 34.0 31.0 34.0 31.0 34.0 4 36.16180910345334 37.0 35.0 37.0 35.0 37.0 5 36.140745100338926 37.0 35.0 37.0 35.0 37.0 6 36.193929712234336 37.0 37.0 37.0 35.0 37.0 7 36.14359951192388 37.0 36.0 37.0 35.0 37.0 8 36.128642819141234 37.0 36.0 37.0 35.0 37.0 9 37.878540547835065 39.0 38.0 39.0 35.0 39.0 10-11 37.8171406709139 39.0 38.0 39.0 35.0 39.0 12-13 37.76362469292113 39.0 38.0 39.0 35.0 39.0 14-15 39.03733697529874 40.0 38.0 41.0 35.5 41.0 16-17 38.98541600575969 40.0 38.0 41.0 35.5 41.0 18-19 39.06758102042388 40.0 38.5 41.0 36.0 41.0 20-21 39.04261763673091 40.0 39.0 41.0 35.0 41.0 22-23 38.922452914573256 40.0 38.0 41.0 35.0 41.0 24-25 39.035637399291915 40.0 39.0 41.0 35.0 41.0 26-27 38.92718459635158 40.0 38.0 41.0 35.0 41.0 28-29 38.81604412468126 40.0 38.0 41.0 35.0 41.0 30-31 38.664474162982664 40.0 38.0 41.0 35.0 41.0 32-33 38.45314702409929 40.0 38.0 41.0 34.0 41.0 34-35 38.27483744337985 40.0 38.0 41.0 34.0 41.0 36-37 38.09112567677463 40.0 37.0 41.0 33.5 41.0 38-39 37.920686570770684 40.0 37.0 41.0 33.0 41.0 40-41 37.730107259461064 40.0 37.0 41.0 33.0 41.0 42-43 37.68805661909079 40.0 37.0 41.0 33.0 41.0 44-45 37.46765082633095 40.0 37.0 41.0 33.0 41.0 46-47 37.11577818395835 40.0 36.5 41.0 32.0 41.0 48-49 36.775747110438175 40.0 36.0 41.0 31.0 41.0 50-51 36.5215741232046 40.0 35.5 41.0 31.0 41.0 52-53 36.282117959265285 39.5 35.0 41.0 30.0 41.0 54-55 36.08030611444636 39.0 35.0 41.0 30.0 41.0 56-57 35.8355378481552 39.0 35.0 41.0 29.5 41.0 58-59 35.574698326141956 38.5 35.0 41.0 29.0 41.0 60-61 35.28312830792035 38.0 35.0 40.0 28.0 41.0 62-63 34.989892980761695 37.5 35.0 40.0 28.0 41.0 64-65 34.617397214687784 37.0 34.0 40.0 28.0 41.0 66-67 34.275686701480126 36.0 34.0 39.5 27.0 41.0 68-69 33.93235857061808 36.0 34.0 39.0 26.0 41.0 70-71 33.55892558252252 35.0 34.0 39.0 26.0 40.5 72-73 33.174705179555914 35.0 33.5 37.5 26.0 40.0 74-75 32.7744174068236 35.0 33.0 37.0 25.5 39.0 76-77 32.045297180279405 35.0 32.0 36.0 24.0 39.0 78-79 32.08485974876939 35.0 33.0 36.0 24.0 38.5 80-81 31.942865843362043 35.0 33.0 36.0 24.0 37.0 82-83 31.684576568530957 35.0 33.0 35.0 24.0 37.0 84-85 31.421669364462435 35.0 33.0 35.0 23.5 36.5 86-87 31.032514856717725 35.0 32.0 35.0 20.5 36.0 88-89 30.541793107443866 35.0 31.5 35.0 17.0 36.0 90-91 30.33288126707613 34.0 31.0 35.0 15.0 35.5 92-93 30.075334916448405 34.0 31.0 35.0 10.0 35.0 94-95 29.951695442762336 34.0 31.0 35.0 4.5 35.0 96-97 29.725988852250993 34.0 31.0 35.0 2.0 35.0 98-99 29.338271088555295 34.0 31.0 35.0 2.0 35.0 100-101 26.207011254436175 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05144511173413946 1101 2 0.05201920160786244 1101 3 0.11025248624646622 1101 4 0.05164253201382962 1101 5 -0.04517978513484877 1101 6 -0.016933526128809717 1101 7 0.08511348938947094 1101 8 0.030587270998367444 1101 9 0.09751327776493923 1101 10-11 0.06489831087001363 1101 12-13 0.18678643666110872 1101 14-15 0.17561698460903585 1101 16-17 0.27848914224126275 1101 18-19 0.17861351653599655 1101 20-21 0.20686552051589047 1101 22-23 0.2528742904043142 1101 24-25 0.15090706367764994 1101 26-27 0.22892505980112787 1101 28-29 0.15924069707187272 1101 30-31 0.15732603096045494 1101 32-33 0.1487003696614977 1101 34-35 0.1089294286644531 1101 36-37 0.0857063995839411 1101 38-39 0.005717295333347749 1101 40-41 -0.07283393037067754 1101 42-43 -0.12499832588128612 1101 44-45 -0.024542551621792086 1101 46-47 0.22574433952951267 1101 48-49 0.18331502837524738 1101 50-51 0.10651431768430086 1101 52-53 0.12672623742246714 1101 54-55 0.1935432222272837 1101 56-57 0.028350394308951365 1101 58-59 -0.02144840470560183 1101 60-61 0.08068511724710703 1101 62-63 0.0550316829553239 1101 64-65 0.09514384120461727 1101 66-67 0.2120317052983367 1101 68-69 0.19051169634410314 1101 70-71 0.14588508364258956 1101 72-73 0.19664838414510655 1101 74-75 0.240040176948618 1101 76-77 0.1360602170369205 1101 78-79 3.5594811559036543E-4 1101 80-81 0.14424672401919025 1101 82-83 0.07620838793048534 1101 84-85 0.2971931501139302 1101 86-87 0.16217627579363736 1101 88-89 0.054458263295945386 1101 90-91 0.35861896779805136 1101 92-93 0.34320631681437774 1101 94-95 0.15185176227904762 1101 96-97 0.06416536996622568 1101 98-99 0.13635688353470954 1101 100-101 0.5634536819655978 1102 1 0.027337316345651175 1102 2 0.10361908891420057 1102 3 0.09688894320251507 1102 4 0.043776988936400585 1102 5 0.09607153287884529 1102 6 0.09402160840621576 1102 7 0.018622836826246214 1102 8 0.039871247857114156 1102 9 0.08335125552746803 1102 10-11 0.13665613666346843 1102 12-13 0.17953282851145502 1102 14-15 0.20654149101639518 1102 16-17 0.2809923222107997 1102 18-19 0.27154429083068266 1102 20-21 0.23477568079751165 1102 22-23 0.2625188032831858 1102 24-25 0.25638477041751173 1102 26-27 0.2723389663100164 1102 28-29 0.24386345226442785 1102 30-31 0.25313656056484746 1102 32-33 0.3267594864110066 1102 34-35 0.346193511187451 1102 36-37 0.24975935444511066 1102 38-39 0.24641239762237177 1102 40-41 0.17745198794315797 1102 42-43 0.26911252395162677 1102 44-45 0.34133665416687364 1102 46-47 0.495862179716525 1102 48-49 0.4376630788307665 1102 50-51 0.3369013673978145 1102 52-53 0.37447933789277243 1102 54-55 0.3770037543421836 1102 56-57 0.4335890454439877 1102 58-59 0.4527070204897967 1102 60-61 0.4785460298425761 1102 62-63 0.5060552705188499 1102 64-65 0.44153143764975056 1102 66-67 0.5966050872796202 1102 68-69 0.5545617222859605 1102 70-71 0.4477635669644897 1102 72-73 0.41952415054565506 1102 74-75 0.5137243250794015 1102 76-77 0.34872486414398907 1102 78-79 0.4482654668244095 1102 80-81 0.3610205751854636 1102 82-83 0.5526128047408108 1102 84-85 0.5550817061288136 1102 86-87 0.5163112125010834 1102 88-89 0.6405667476778305 1102 90-91 0.7543958148303602 1102 92-93 0.650988664636099 1102 94-95 0.599786734691417 1102 96-97 0.5941048529143416 1102 98-99 0.5821129491871311 1102 100-101 0.9754013017305034 1103 1 -0.0640931979008883 1103 2 0.0754894934784005 1103 3 0.060770890948610656 1103 4 0.019003098198588475 1103 5 -0.0018285257215993056 1103 6 0.06604957076925189 1103 7 0.12651123062123304 1103 8 0.09807779783887582 1103 9 0.07599855824852142 1103 10-11 0.09617069279274659 1103 12-13 0.1700206219283018 1103 14-15 0.2910519008878083 1103 16-17 0.28591600342437573 1103 18-19 0.14543931310991098 1103 20-21 0.16175109625255146 1103 22-23 0.17750669621023007 1103 24-25 0.184490732183491 1103 26-27 0.2060672537396755 1103 28-29 0.19929809065546067 1103 30-31 0.19545934950374289 1103 32-33 0.3272452896240736 1103 34-35 0.4142681548856899 1103 36-37 0.26523595296572466 1103 38-39 0.30809904003803723 1103 40-41 0.4124701761943257 1103 42-43 0.33193731415097005 1103 44-45 0.363325641705984 1103 46-47 0.5152991455402969 1103 48-49 0.49411990799388406 1103 50-51 0.606002486334603 1103 52-53 0.6338747582127482 1103 54-55 0.6077236552742704 1103 56-57 0.5583796594218526 1103 58-59 0.6077169438669543 1103 60-61 0.6113686363461213 1103 62-63 0.7134994618880057 1103 64-65 0.627356349472663 1103 66-67 0.35296130058523545 1103 68-69 0.365596020131008 1103 70-71 0.3288841346148317 1103 72-73 0.4040883235607069 1103 74-75 0.567279464780654 1103 76-77 0.4264257365166344 1103 78-79 0.33856999564366674 1103 80-81 0.38156597394490177 1103 82-83 0.4362791747196937 1103 84-85 0.3252795102706507 1103 86-87 0.3428773923049384 1103 88-89 0.5753468025190038 1103 90-91 0.6306403832325564 1103 92-93 0.639024637569328 1103 94-95 0.5983604219861753 1103 96-97 0.5866630110913107 1103 98-99 0.5079868421362832 1103 100-101 0.8496632678306995 1104 1 -0.01314928776737645 1104 2 0.058964785748095494 1104 3 0.043271730538094744 1104 4 -0.019190951425777314 1104 5 0.05244333203210516 1104 6 0.016911769673946253 1104 7 0.07570482805724765 1104 8 0.048181060734833636 1104 9 0.05701193614840605 1104 10-11 0.09922494746205501 1104 12-13 0.1603593929808511 1104 14-15 0.2127856333456748 1104 16-17 0.21017577637822882 1104 18-19 0.20526171679511407 1104 20-21 0.19059066458455476 1104 22-23 0.12385494470755987 1104 24-25 0.16160563826845475 1104 26-27 0.18655506184256865 1104 28-29 0.2102458086909067 1104 30-31 0.14252488508250138 1104 32-33 0.20461762574547038 1104 34-35 0.27679694553306433 1104 36-37 0.2783447875626308 1104 38-39 0.4221357862916122 1104 40-41 0.4374787043080133 1104 42-43 0.3749983384237012 1104 44-45 0.36470270068350885 1104 46-47 0.2827150080961971 1104 48-49 0.41227579926771085 1104 50-51 0.4449632728779065 1104 52-53 0.44510264792110377 1104 54-55 0.43479135924162193 1104 56-57 0.5112839779528429 1104 58-59 0.5719186992039624 1104 60-61 0.5116088779363466 1104 62-63 0.36572298573101847 1104 64-65 0.28871288808981177 1104 66-67 0.3186862166923987 1104 68-69 0.4115281760117071 1104 70-71 0.5230130902712844 1104 72-73 0.3609763950434868 1104 74-75 0.3657548496035261 1104 76-77 0.3565593304091905 1104 78-79 0.294092246748356 1104 80-81 0.4372234848992278 1104 82-83 0.4099246590504393 1104 84-85 0.4080689835373299 1104 86-87 0.5223075820918304 1104 88-89 0.4927278915260622 1104 90-91 0.7143071283490521 1104 92-93 0.7573175075374117 1104 94-95 0.7890285501114995 1104 96-97 0.7492610843987109 1104 98-99 0.6959167185645789 1104 100-101 0.6153807796841164 1105 1 -0.10783009691881773 1105 2 -0.028699429368145957 1105 3 -0.0025553595389098405 1105 4 0.013388997204771158 1105 5 -0.049519687556148995 1105 6 0.011317303645526522 1105 7 -0.056582942531520075 1105 8 0.08104505369917803 1105 9 -0.03918377611658741 1105 10-11 0.03541441964794245 1105 12-13 0.008083352724071347 1105 14-15 0.05716015755552917 1105 16-17 -0.06474323603711696 1105 18-19 -0.02198455281951084 1105 20-21 0.026477219692331744 1105 22-23 -0.027106675628743915 1105 24-25 -0.02635958758808954 1105 26-27 0.0276033442957484 1105 28-29 0.010372961422874027 1105 30-31 0.04929543445093287 1105 32-33 0.05802247216453793 1105 34-35 -0.028271117081239083 1105 36-37 -0.011613641564181876 1105 38-39 -0.03901845041708185 1105 40-41 0.004546845717605663 1105 42-43 -0.008990838661098621 1105 44-45 -0.10427196917055426 1105 46-47 -0.28093729367962084 1105 48-49 -0.30045180158074203 1105 50-51 -0.20668526451618163 1105 52-53 -0.33489484842190365 1105 54-55 -0.2792198423993568 1105 56-57 -0.2893756784742507 1105 58-59 -0.1406160438026376 1105 60-61 -0.1484599535675386 1105 62-63 -0.08005123524402791 1105 64-65 -0.19209728006189408 1105 66-67 -0.14366456471546485 1105 68-69 -0.11956228734275953 1105 70-71 -0.13557674081447857 1105 72-73 -0.2526480106557045 1105 74-75 -0.22036364327122016 1105 76-77 -0.2428914536675535 1105 78-79 -0.4621783337569241 1105 80-81 -0.21450702502071906 1105 82-83 -0.20914710694277971 1105 84-85 -0.15358706482773243 1105 86-87 -0.12510729674769294 1105 88-89 -0.08247967688922486 1105 90-91 0.02640417619432256 1105 92-93 -0.08064695578401526 1105 94-95 -0.21400220928452285 1105 96-97 -0.3197165720512558 1105 98-99 -0.35619143687566535 1105 100-101 0.004431601379256023 1106 1 -0.062451389092380793 1106 2 -0.052494545709528495 1106 3 -0.04134886525437054 1106 4 0.059460123649444085 1106 5 -0.0010467716609241506 1106 6 0.0446665927122325 1106 7 0.09961525778217606 1106 8 -0.05081252482259657 1106 9 0.03423547733572008 1106 10-11 0.04461094310438085 1106 12-13 0.05407891522388297 1106 14-15 0.07398924154590958 1106 16-17 0.1013246390505671 1106 18-19 0.19775419462531119 1106 20-21 0.17927353722949135 1106 22-23 0.08677812397627349 1106 24-25 0.10049601904587746 1106 26-27 0.03128038821014201 1106 28-29 0.1297573711661073 1106 30-31 0.18939731030328488 1106 32-33 0.19259222430878253 1106 34-35 0.3339758114400837 1106 36-37 0.1804021696644469 1106 38-39 0.1303796571727318 1106 40-41 0.047824815666636766 1106 42-43 0.11566016730273532 1106 44-45 0.2680023851490887 1106 46-47 0.2910528679549671 1106 48-49 0.08189904994363673 1106 50-51 0.11670493745931765 1106 52-53 -0.015999647485038793 1106 54-55 0.15018235949185055 1106 56-57 0.0631253074696545 1106 58-59 0.05144161359465471 1106 60-61 -0.05951697574601411 1106 62-63 0.049400640156740394 1106 64-65 0.27615803575626074 1106 66-67 0.20124119108955796 1106 68-69 0.23436688726413735 1106 70-71 0.0877803454879782 1106 72-73 0.019440895894881294 1106 74-75 0.1176462251993442 1106 76-77 0.038024887110786665 1106 78-79 -0.18012883485727116 1106 80-81 -0.055523495552286306 1106 82-83 0.07365773035389367 1106 84-85 0.1781365457439925 1106 86-87 0.10569991283538016 1106 88-89 0.1968829367201117 1106 90-91 0.2702821419286323 1106 92-93 0.16241305282606078 1106 94-95 0.3231541779761784 1106 96-97 0.39067482509899065 1106 98-99 0.33972931430821873 1106 100-101 0.38311112896913 1107 1 -0.05060800917907926 1107 2 0.022424928267852806 1107 3 -0.0025692280452958016 1107 4 0.004951271841548532 1107 5 0.06659690371627391 1107 6 0.08905217310505975 1107 7 0.046724191932476344 1107 8 0.14288283251919154 1107 9 0.11855409398341976 1107 10-11 0.09845560659037034 1107 12-13 0.10175849430891049 1107 14-15 0.13398506388007547 1107 16-17 0.18670129498043053 1107 18-19 0.07011651329039381 1107 20-21 0.04862490919916951 1107 22-23 -0.020236721873907015 1107 24-25 0.14441751525571078 1107 26-27 0.1263471097717428 1107 28-29 0.11960352408078734 1107 30-31 0.0018594691272468822 1107 32-33 0.043672665567157765 1107 34-35 0.20509360950745759 1107 36-37 0.20012377970053308 1107 38-39 0.2684088281785151 1107 40-41 0.12081378420699451 1107 42-43 -0.07895367106397799 1107 44-45 0.02154280557968491 1107 46-47 0.2626968097340523 1107 48-49 0.27815489262121673 1107 50-51 0.2122299913451542 1107 52-53 0.26355673283998016 1107 54-55 0.32351553136950173 1107 56-57 0.1661719762352405 1107 58-59 0.28368744198340323 1107 60-61 0.38173020887048636 1107 62-63 0.16578253935995946 1107 64-65 0.3463827820690142 1107 66-67 0.29882904316386316 1107 68-69 0.18781739706046352 1107 70-71 0.2994045493096351 1107 72-73 0.2312894222081212 1107 74-75 0.3693996339324812 1107 76-77 0.17345875889882478 1107 78-79 0.02321844421080499 1107 80-81 0.3423210710602973 1107 82-83 0.5210675775360869 1107 84-85 0.5229193071205636 1107 86-87 0.6811521905714493 1107 88-89 0.6321587837569993 1107 90-91 0.3177534431807203 1107 92-93 0.6326925931617282 1107 94-95 0.6133645143690991 1107 96-97 0.5035141847657485 1107 98-99 0.6458686667094717 1107 100-101 0.909812770973442 1108 1 -0.23277140750751357 1108 2 -0.14831148153112395 1108 3 -0.13958972078155085 1108 4 -0.051260312988901546 1108 5 -0.10786952322579424 1108 6 -0.07199827126814995 1108 7 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-0.6909775485071776 2316 78-79 -0.6904529378845083 2316 80-81 -0.7334392939014975 2316 82-83 -0.9152331343803937 2316 84-85 -0.7021481358374189 2316 86-87 -0.9020854730572161 2316 88-89 -0.7928602943998477 2316 90-91 -0.9325099274275352 2316 92-93 -0.8949148804551896 2316 94-95 -0.6778162729228434 2316 96-97 -0.7563813539578916 2316 98-99 -0.9072757147733377 2316 100-101 -1.079315817477248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 13.0 5 40.0 6 82.0 7 158.0 8 338.0 9 576.0 10 954.0 11 1742.0 12 2671.0 13 4242.0 14 5095.0 15 7184.0 16 9655.0 17 14007.0 18 12651.0 19 6800.0 20 5273.0 21 5560.0 22 5611.0 23 6105.0 24 7052.0 25 8454.0 26 10049.0 27 12787.0 28 15932.0 29 19993.0 30 24531.0 31 30453.0 32 38901.0 33 49849.0 34 71229.0 35 112571.0 36 188766.0 37 321719.0 38 348467.0 39 65821.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.20684280258748 18.33074726691748 13.209202145372359 22.25320778512268 2 16.157392554233454 20.40328291001395 38.9967818210342 24.4425427147184 3 20.25367523155001 25.45145981249907 28.81719260142169 25.477672354529226 4 12.487414800406684 15.530542557227772 36.54452281515636 35.43751982720919 5 13.580993575454322 38.622096395740144 32.50878049504258 15.288129533762957 6 32.43530059285563 35.52795662990081 16.80676128146123 15.229981495782324 7 29.513132059634593 33.35874513283965 19.42420018186276 17.703922625662997 8 27.741065303143458 33.67852401485707 18.904047258881707 19.676363423117767 9 28.246168976926604 15.672485945202364 19.68088526325235 36.400459814618685 10-11 26.230370524239305 26.003429550088814 25.833329800628256 21.932870125043628 12-13 27.405700986645364 23.382106847662932 27.14640144162069 22.065790724071014 14-15 23.796360342614175 24.80935851338853 26.163543653067467 25.230737490929823 16-17 22.50825770395204 28.329877167757683 26.58083364833309 22.581031479957183 18-19 22.280978667512628 26.38786931599396 29.454842714149756 21.876309302343657 20-21 23.38079975186376 25.272414661658253 29.091925221402597 22.25486036507539 22-23 22.795098747485167 25.30450894741769 29.144612384547315 22.75577992054983 24-25 22.597228620443094 25.593256385110408 29.48823025681752 22.32128473762898 26-27 22.854105682181896 25.431015905248216 29.75781431606585 21.957064096504038 28-29 21.97043001920766 25.83414851971691 30.093925722124425 22.101495738951 30-31 22.347237290249236 25.752848872762417 29.653818286129734 22.24609555085861 32-33 21.90536818766966 25.984888415346724 29.724454511925774 22.385288885057843 34-35 22.71609962616519 25.571650730465173 29.447634510937725 22.26461513243192 36-37 23.098670860676354 25.40346853644888 29.275438194880145 22.222422407994618 38-39 22.80791125310965 25.38186640628367 29.478730011142112 22.331492329464563 40-41 22.44813966962002 25.800196419234954 29.62348269673718 22.128181214407846 42-43 22.422881545446163 26.193725528710566 28.72056335500473 22.662829570838543 44-45 22.315886088001324 26.129533581740223 28.54335750631233 23.011222823946124 46-47 22.691370717996822 26.46297837158893 27.92303303000913 22.922617880405117 48-49 23.23257698054583 26.24816582594099 27.67388394063795 22.84537325287523 50-51 22.80742971710195 26.484380106164053 27.741284743996253 22.966905432737743 52-53 23.131607722324187 26.70638881185093 27.38814235672559 22.773861109099293 54-55 22.683907197316277 26.21565594868255 27.57712419639848 23.523312657602695 56-57 22.98630445741918 26.980187089159337 26.915961081441615 23.117547371979867 58-59 23.364045873449168 26.17890475603367 27.128260329669047 23.328789040848115 60-61 23.09943483105342 26.221253150327588 27.940076123643337 22.739235894975657 62-63 23.4028189434014 26.285421375444855 27.57305068064292 22.738709000510827 64-65 22.93459813953094 26.55976435377456 27.619437563500554 22.88619994319394 66-67 23.010054735461754 26.650559966312294 27.246524365299678 23.092860932926275 68-69 22.656079437426566 26.750181756777287 27.640701648281386 22.953037157514768 70-71 22.89210042614946 26.71907307634833 27.403639456995926 22.98518704050628 72-73 22.994422494291076 26.72327545275724 27.021121605733793 23.26118044721789 74-75 22.696987959894106 27.43630034344789 26.78593255534132 23.08077914131669 76-77 22.891760315667366 26.859690923784395 27.06868214820607 23.179866612342163 78-79 22.98092164314497 26.676632708563652 27.287503686869652 23.054941961421726 80-81 22.686688225664344 27.462993017230474 27.092481599840607 22.757837157264575 82-83 22.891639046937406 27.291495478513134 26.687829820546533 23.129035654002923 84-85 22.790069838120683 26.802591171266076 26.504677836385415 23.902661154227822 86-87 22.731067023673514 26.697733808242045 26.93422137018474 23.636977797899696 88-89 22.944004480665047 26.741339679046778 26.78107146461739 23.533584375670785 90-91 22.893532874289907 26.775828280429938 26.93413093014767 23.396507915132485 92-93 22.754544059814812 26.94780979133767 26.728663141659442 23.56898300718808 94-95 22.623240276277816 26.957471289950043 26.803265281320865 23.616023152451273 96-97 22.314592348517525 26.875891361709236 26.743447862162668 24.066068427610567 98-99 22.595322116313664 26.84807211375241 26.43212363003548 24.124482139898447 100-101 22.67525351637501 26.845904789830023 26.529903539249332 23.948938154545633 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 884.0 1 619.0 2 648.5 3 1013.5 4 769.5 5 657.0 6 1474.0 7 2838.0 8 3997.5 9 4061.5 10 3126.0 11 2317.0 12 2141.5 13 2261.5 14 2367.0 15 2402.5 16 2392.5 17 2424.5 18 2547.5 19 2638.5 20 2767.5 21 3041.0 22 3480.0 23 4085.0 24 4678.5 25 5517.5 26 6971.5 27 8507.5 28 9809.0 29 11283.5 30 13507.0 31 16803.5 32 19583.0 33 21529.5 34 23801.0 35 25635.0 36 28709.0 37 32365.5 38 33230.5 39 32678.5 40 32790.5 41 34211.5 42 35450.5 43 37432.0 44 40394.0 45 43420.5 46 44861.0 47 48316.0 48 66285.0 49 66856.0 50 53251.0 51 49773.5 52 46370.0 53 47419.5 54 48767.0 55 47947.0 56 43850.5 57 38786.0 58 37338.0 59 35645.5 60 32279.0 61 28369.5 62 24587.5 63 19911.5 64 15499.5 65 12860.0 66 9139.5 67 7116.0 68 5011.5 69 2611.0 70 1972.0 71 1603.0 72 1367.5 73 1374.0 74 1101.0 75 883.5 76 691.5 77 366.0 78 169.5 79 50.5 80 35.0 81 34.5 82 16.0 83 5.5 84 2.5 85 1.0 86 2.5 87 2.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27858774454146773 2 0.018864551811456222 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.945762647198261E-4 12-13 1.0598062815424844E-4 14-15 0.0 16-17 5.652300168226584E-4 18-19 0.0 20-21 1.0598062815424844E-4 22-23 3.532687605141615E-5 24-25 0.0020489588109821365 26-27 0.002402227571496298 28-29 0.0022962469433420495 30-31 8.831719012854037E-4 32-33 0.0010598062815424844 34-35 0.0015543825462623103 36-37 7.06537521028323E-5 38-39 1.413075042056646E-4 40-41 9.184987773368198E-4 42-43 0.0032853994727817014 44-45 0.00476912826694118 46-47 0.014166077296617875 48-49 0.01458999980923487 50-51 0.006394164565306323 52-53 0.007453970846848807 54-55 4.2392251261699375E-4 56-57 0.0027201694559590432 58-59 0.0017310169265193915 60-61 0.001695690050467975 62-63 0.0 64-65 7.771912731311552E-4 66-67 0.0 68-69 0.0 70-71 3.1794188446274536E-4 72-73 0.0017663438025708074 74-75 0.0 76-77 0.20161048162543196 78-79 0.10965462326359572 80-81 8.478450252339875E-4 82-83 0.0 84-85 6.005568928740745E-4 86-87 0.12350275867575086 88-89 0.5948339389537451 90-91 0.31426788935339806 92-93 0.2965691244516386 94-95 0.0027201694559590432 96-97 0.0022962469433420495 98-99 0.002366900695444882 100-101 7.418643970797392E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1415353.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.85329364436767 #Duplication Level Percentage of deduplicated Percentage of total 1 62.628228057046755 19.949153387275363 2 14.703127467911232 9.366860734518891 3 6.780628420049692 6.479560444715631 4 4.012080750578123 5.1119194509232 5 2.5195722830463576 4.012833789504274 6 1.8057237999541687 3.4510950264338134 7 1.2933003164198262 2.883711232449205 8 0.9776809420912166 2.4913886511146837 9 0.746073494178054 2.138840828129969 >10 4.106530884065506 23.420413897393647 >50 0.26368706370585476 5.778776028801827 >100 0.14962334497915536 8.711675062697816 >500 0.008201572758731535 1.9403599200834325 >1k 0.005098274958130413 3.1838255346021667 >5k 4.4332825722873164E-4 1.0795860113560527 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8684 0.6135571832609957 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6606 0.46673868639131016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4620 0.3264203347150852 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4274 0.3019741364875052 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4216 0.29787621886554094 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3717 0.26261999656622764 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2276 0.1608079397860463 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 1880 0.13282905395332473 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 1865 0.13176924767178225 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1807 0.12767133004981795 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1806 0.12760067629771513 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1781 0.12583433249514434 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1657 0.11707326723439312 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1560 0.11021985328041838 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1539 0.1087361244862589 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1526 0.10781762570892209 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.06537521028323E-5 0.0 12-13 0.0 0.0 0.0 3.5326876051416147E-4 0.0 14-15 0.0 0.0 0.0 4.945762647198261E-4 0.0 16-17 0.0 0.0 0.0 0.0019429781828278882 0.0 18-19 0.0 0.0 0.0 0.0026848425799076273 0.0 20-21 0.0 0.0 0.0 0.004168571374067106 0.0 22-23 0.0 0.0 0.0 0.007913220235517217 0.0 24-25 0.0 0.0 0.0 0.011834503477224409 0.0 26-27 0.0 0.0 0.0 0.013600847279795217 0.0 28-29 3.532687605141615E-5 0.0 0.0 0.015897094223137266 0.0 30-31 7.06537521028323E-5 0.0 0.0 0.0214434137632096 0.0 32-33 7.06537521028323E-5 0.0 0.0 0.02907401899031549 0.0 34-35 7.06537521028323E-5 0.0 0.0 0.04214496312933946 0.0 36-37 7.06537521028323E-5 0.0 0.0 0.0631997812559835 0.0 38-39 7.06537521028323E-5 0.0 0.0 0.09400481717281838 0.0 40-41 7.06537521028323E-5 0.0 0.0 0.12904907821582318 0.0 42-43 7.06537521028323E-5 0.0 0.0 0.16246830296046288 0.0 44-45 7.06537521028323E-5 0.0 0.0 0.2010805784846607 0.0 46-47 7.06537521028323E-5 0.0 0.0 0.25173931874239147 0.0 48-49 7.06537521028323E-5 0.0 0.0 0.3103819329877423 0.0 50-51 7.06537521028323E-5 0.0 0.0 0.36757614531498506 0.0 52-53 7.06537521028323E-5 0.0 0.0 0.4241344738733023 0.0 54-55 7.06537521028323E-5 0.0 0.0 0.48443745129306964 0.0 56-57 7.06537521028323E-5 0.0 0.0 0.555868394669033 0.0 58-59 7.06537521028323E-5 0.0 0.0 0.6407235509445346 0.0 60-61 7.06537521028323E-5 0.0 0.0 0.7411225326826594 0.0 62-63 7.06537521028323E-5 0.0 0.0 0.8363991173933287 0.0 64-65 7.06537521028323E-5 0.0 0.0 0.9262000363160285 0.0 66-67 7.06537521028323E-5 0.0 0.0 1.0306262819240146 0.0 68-69 7.06537521028323E-5 0.0 0.0 1.157626401328856 0.0 70-71 7.06537521028323E-5 0.0 0.0 1.2996404430555488 0.0 72-73 7.06537521028323E-5 0.0 0.0 1.4490378018769876 0.0 74-75 7.06537521028323E-5 0.0 0.0 1.5911224973557831 0.0 76-77 7.06537521028323E-5 0.0 0.0 1.7536261271922977 0.0 78-79 7.06537521028323E-5 0.0 0.0 1.951279998699971 0.0 80-81 7.06537521028323E-5 0.0 0.0 2.1692114970611573 0.0 82-83 7.06537521028323E-5 0.0 0.0 2.3901104530106623 0.0 84-85 7.06537521028323E-5 0.0 0.0 2.6133763096556124 0.0 86-87 7.06537521028323E-5 0.0 0.0 2.8496424566874836 0.0 88-89 7.06537521028323E-5 0.0 0.0 3.11173255011294 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGCT 95 3.5254678E-5 29.957361 3 TATAACG 135 4.2712418E-7 28.114103 2 GTACATG 10170 0.0 27.880934 1 TACATGG 10455 0.0 26.727556 2 ACATGGG 10095 0.0 26.594145 3 CATGGGG 3505 0.0 26.388975 4 TAGTCGA 120 1.7276683E-4 23.716242 5 TACCTGG 1200 0.0 23.325918 2 CCGTAAG 205 2.6258931E-8 23.207487 1 TATAGGG 225 2.8539944E-9 23.194134 2 ATGGGAG 2125 0.0 22.767595 5 ATAGCGC 125 2.2780932E-4 22.767593 8 ATGGGGG 1620 0.0 22.25228 5 GTACCGT 150 3.0673495E-5 22.135162 6 CATGGGA 6305 0.0 22.042377 4 GTATAGG 390 0.0 21.957853 1 GAGTACT 7265 0.0 21.741251 12-13 AGTACTT 7645 0.0 20.722633 12-13 GTCCAAG 1105 0.0 20.666216 1 GTACTTT 7665 0.0 20.390093 14-15 >>END_MODULE