FastQCFastQC Report
Thu 26 May 2016
SRR936338_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936338_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559412
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44310.17312570230974927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31330.12241092876019961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33800.047.8997421
GTATCAA58300.040.4375231
ATCAACG70850.032.5313843
TCAACGC71950.032.0998084
CAACGCA72700.031.8337555
TATCAAC74300.031.1500632
AACGCAG76300.030.2673766
GTACATG95800.026.931141
TACATGG93250.026.6978192
GTACCTG24050.024.9984111
ACATGGG97450.024.8171833
ACGCAGA92800.024.580777
GTGTAGC18550.024.2439561
CGCAGAG93850.024.0536258
GTATAGG7300.023.345511
TACCTGG24050.023.222332
CATGGGG61450.021.5650044
GAGTACA71500.020.9220771
AGTACAT67100.020.45572
TAGCCCT21200.020.0918564
Produced by FastQC (version 0.11.5)