##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936338_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2559412 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56340245337601 33.0 31.0 34.0 30.0 34.0 2 31.66159883598264 33.0 31.0 34.0 30.0 34.0 3 31.729731672743583 34.0 31.0 34.0 30.0 34.0 4 35.2200083456669 37.0 35.0 37.0 33.0 37.0 5 35.19639471878697 37.0 35.0 37.0 33.0 37.0 6 35.28270477750358 37.0 35.0 37.0 33.0 37.0 7 35.22357244554609 37.0 35.0 37.0 32.0 37.0 8 35.222030685173 37.0 35.0 37.0 32.0 37.0 9 36.86798256787106 39.0 37.0 39.0 33.0 39.0 10-11 36.779895343149136 39.0 37.0 39.0 33.0 39.0 12-13 36.67567374850161 39.0 37.0 39.0 32.0 39.0 14-15 37.89085422745537 40.0 38.0 41.0 33.0 41.0 16-17 37.82843735201679 40.0 38.0 41.0 33.0 41.0 18-19 37.77166396031589 40.0 38.0 41.0 32.0 41.0 20-21 37.679067692110536 40.0 38.0 41.0 32.0 41.0 22-23 37.511820293098566 40.0 37.5 41.0 32.0 41.0 24-25 37.68235457988007 40.0 38.0 41.0 32.0 41.0 26-27 37.63561142168592 40.0 38.0 41.0 32.0 41.0 28-29 37.498098782064005 40.0 38.0 41.0 32.0 41.0 30-31 37.35324441707705 40.0 37.0 41.0 31.5 41.0 32-33 37.17028149434323 40.0 37.0 41.0 31.0 41.0 34-35 36.905875646437536 40.0 37.0 41.0 30.5 41.0 36-37 36.71193324873057 40.0 36.5 41.0 30.0 41.0 38-39 36.54759198597178 40.0 36.0 41.0 30.0 41.0 40-41 36.37069549568416 40.0 36.0 41.0 30.0 41.0 42-43 36.18057682780264 40.0 35.5 41.0 29.0 41.0 44-45 36.00044521944884 39.0 35.0 41.0 28.5 41.0 46-47 35.74303472828915 39.0 35.0 41.0 27.5 41.0 48-49 35.57958917907707 39.0 35.0 41.0 27.0 41.0 50-51 35.278033587402106 38.5 35.0 40.5 26.5 41.0 52-53 35.30267694298534 39.0 35.0 40.0 27.0 41.0 54-55 35.38455141259008 39.0 35.0 41.0 27.0 41.0 56-57 35.21776915947882 39.0 35.0 41.0 26.0 41.0 58-59 34.9778195147948 38.5 35.0 41.0 26.0 41.0 60-61 34.671220967941075 38.0 34.0 40.0 26.0 41.0 62-63 34.355014354859634 37.5 34.0 40.0 25.5 41.0 64-65 34.017619867375785 37.0 34.0 40.0 24.5 41.0 66-67 33.65560878826855 36.5 34.0 39.5 23.5 41.0 68-69 33.263700607795855 36.0 33.0 39.0 22.5 41.0 70-71 32.86161294078484 35.5 33.0 39.0 22.0 40.5 72-73 32.45667286079772 35.0 32.5 38.0 20.5 40.0 74-75 32.03411037378898 35.0 32.0 37.0 19.5 39.5 76-77 31.627619351632326 35.0 32.0 37.0 18.0 39.0 78-79 31.232251196759258 35.0 31.5 36.0 17.0 39.0 80-81 30.833696958520157 35.0 31.0 36.0 12.0 37.5 82-83 30.476990027396916 35.0 31.0 35.0 7.5 37.0 84-85 30.151663350800888 34.0 31.0 35.0 6.5 37.0 86-87 29.818422942457097 34.0 30.5 35.0 2.0 36.0 88-89 29.482815388847126 34.0 30.0 35.0 2.0 36.0 90-91 29.11815545914452 34.0 30.0 35.0 2.0 35.5 92-93 28.78594263057296 34.0 29.0 35.0 2.0 35.0 94-95 28.393884415639217 34.0 29.0 35.0 2.0 35.0 96-97 27.946689708417402 34.0 29.0 35.0 2.0 35.0 98-99 27.411538079840213 34.0 28.0 35.0 2.0 35.0 100-101 24.091444245787706 29.5 14.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4141414763021274 1101 2 0.35188779705603324 1101 3 0.371320869901961 1101 4 0.18746980791328838 1101 5 0.38368182741712786 1101 6 0.39854360130321 1101 7 0.5183902637750677 1101 8 0.3634149118798575 1101 9 0.4687535907395244 1101 10-11 0.6170098078590698 1101 12-13 0.610433681218673 1101 14-15 0.6381246827467564 1101 16-17 0.6928236571930668 1101 18-19 0.7016693226154018 1101 20-21 0.7224071789794166 1101 22-23 0.7674001127728047 1101 24-25 0.696179585620456 1101 26-27 0.7026946347105891 1101 28-29 0.6614619484395732 1101 30-31 0.7432308527241815 1101 32-33 0.8075076849432534 1101 34-35 0.6877089860567196 1101 36-37 0.691061402420921 1101 38-39 0.6511279252258504 1101 40-41 0.6723182242446555 1101 42-43 0.6621499219516664 1101 44-45 0.6453482841603275 1101 46-47 0.704406628449 1101 48-49 0.8279283170466272 1101 50-51 0.8055284358676147 1101 52-53 0.8555908293883334 1101 54-55 0.8404816825798136 1101 56-57 0.9116051717886862 1101 58-59 0.9728835840879384 1101 60-61 0.938247588854999 1101 62-63 0.9627389176273411 1101 64-65 0.8775003003696682 1101 66-67 1.0066325377466327 1101 68-69 0.9913136773556772 1101 70-71 0.9978910360329181 1101 72-73 1.1109321208616336 1101 74-75 0.8803699380307677 1101 76-77 0.8565438035422801 1101 78-79 0.9822502963672655 1101 80-81 0.9324768270400838 1101 82-83 1.132220726247926 1101 84-85 1.1815882023664166 1101 86-87 1.15416995254839 1101 88-89 1.0890981365838748 1101 90-91 1.0773087846236429 1101 92-93 1.08720723028949 1101 94-95 1.4028301121250912 1101 96-97 1.4577775037722702 1101 98-99 1.5999478894910162 1101 100-101 1.6345905806985002 1102 1 0.2613731888307811 1102 2 0.297782385715049 1102 3 0.2818983782185178 1102 4 0.21952311156830717 1102 5 0.35906790831796087 1102 6 0.3084981773321829 1102 7 0.16647637049968722 1102 8 0.1668278799931926 1102 9 0.37419814462402456 1102 10-11 0.3208195025213101 1102 12-13 0.35522912099629167 1102 14-15 0.45657800910232993 1102 16-17 0.4150263274796302 1102 18-19 0.39526138518971976 1102 20-21 0.35638970640480494 1102 22-23 0.43995806966154305 1102 24-25 0.48859687521941453 1102 26-27 0.36676711500566483 1102 28-29 0.38001538959294834 1102 30-31 0.48620110661241256 1102 32-33 0.22371548418499998 1102 34-35 0.21875999908821342 1102 36-37 0.2789939794752243 1102 38-39 0.3286674360059507 1102 40-41 0.346956748312202 1102 42-43 0.3279692216927401 1102 44-45 0.28510793246925203 1102 46-47 0.40213230666628164 1102 48-49 0.3189643576120744 1102 50-51 0.28703395522669695 1102 52-53 0.3078904226826893 1102 54-55 0.37247442601855596 1102 56-57 0.40885682348288555 1102 58-59 0.327756739000975 1102 60-61 0.3669585018059607 1102 62-63 0.44174109093925296 1102 64-65 0.3078602413482159 1102 66-67 0.3696737994406547 1102 68-69 0.4835926306618461 1102 70-71 0.6386238375028199 1102 72-73 0.4265677784122701 1102 74-75 0.5230887527568768 1102 76-77 0.4154865097688685 1102 78-79 0.426056985871611 1102 80-81 0.3691098923087601 1102 82-83 0.4880345641430779 1102 84-85 0.5309519011254302 1102 86-87 0.4311358171540327 1102 88-89 0.29147095441646087 1102 90-91 0.45760551825696183 1102 92-93 0.6253745448362054 1102 94-95 0.663790559067106 1102 96-97 0.661823916512617 1102 98-99 0.6394898066827288 1102 100-101 0.7916444556763125 1103 1 0.3699010868593966 1103 2 0.26372079569334517 1103 3 0.3872219194130686 1103 4 0.24864223015622855 1103 5 0.2163793877221849 1103 6 0.22471608331330373 1103 7 0.3164759220504294 1103 8 0.2451611286194435 1103 9 0.13252379203527198 1103 10-11 0.29987262432611317 1103 12-13 0.3795434794447843 1103 14-15 0.4436173582828644 1103 16-17 0.38549575669340896 1103 18-19 0.32373512923282277 1103 20-21 0.43132212136388404 1103 22-23 0.46152156559261925 1103 24-25 0.4762170596217459 1103 26-27 0.5275395389636159 1103 28-29 0.42406422497021623 1103 30-31 0.4307850167013285 1103 32-33 0.4314755810201447 1103 34-35 0.24825422679878528 1103 36-37 0.36038696004840887 1103 38-39 0.2762594129496776 1103 40-41 0.36848184577517173 1103 42-43 0.32899551651524916 1103 44-45 0.4739734604734167 1103 46-47 0.4852117367470683 1103 48-49 0.4268593816190531 1103 50-51 0.46940584448364575 1103 52-53 0.48204279752049217 1103 54-55 0.5560225603201374 1103 56-57 0.5445437431148008 1103 58-59 0.5584428701095945 1103 60-61 0.5554244428318995 1103 62-63 0.6057889696247614 1103 64-65 0.4378583952320767 1103 66-67 0.4952835035837353 1103 68-69 0.36708952963519437 1103 70-71 0.4681695808224191 1103 72-73 0.5078341654957441 1103 74-75 0.44645409913908907 1103 76-77 0.4891805507750604 1103 78-79 0.5194979295882369 1103 80-81 0.4573657348552338 1103 82-83 0.6292420810496182 1103 84-85 0.6292516057468482 1103 86-87 0.6369323602081494 1103 88-89 0.5116197424075857 1103 90-91 0.6526803868613271 1103 92-93 0.6942628914336701 1103 94-95 0.660382811807132 1103 96-97 0.7853911211190869 1103 98-99 0.9101005347849522 1103 100-101 1.0206527325548684 1104 1 0.43467790366923964 1104 2 0.30569026400075217 1104 3 0.35265609633436057 1104 4 0.4321646935878505 1104 5 0.4118823480193754 1104 6 0.25744042740431894 1104 7 0.28786552037873037 1104 8 0.3523558116935064 1104 9 0.3685857260961072 1104 10-11 0.4489569637735258 1104 12-13 0.3535726761013862 1104 14-15 0.6155794258473009 1104 16-17 0.6002348442253336 1104 18-19 0.5222963377306158 1104 20-21 0.622250406713313 1104 22-23 0.7345785077797586 1104 24-25 0.7641436906874546 1104 26-27 0.643150753265914 1104 28-29 0.6453587745406821 1104 30-31 0.5602436015631795 1104 32-33 0.6805243678787463 1104 34-35 0.6638211817558286 1104 36-37 0.6402816082954459 1104 38-39 0.6244222873402023 1104 40-41 0.7616883990605245 1104 42-43 0.7614477744674204 1104 44-45 0.7371380762152313 1104 46-47 0.811064273315445 1104 48-49 0.7810966697546462 1104 50-51 0.603925957091036 1104 52-53 0.6276886937359549 1104 54-55 0.8393861254466799 1104 56-57 0.8099779200360899 1104 58-59 0.7656890681592827 1104 60-61 0.6719273741065663 1104 62-63 0.7590140973238491 1104 64-65 0.7239348405536461 1104 66-67 0.6617291153784848 1104 68-69 0.6781130088550782 1104 70-71 0.5487462583241651 1104 72-73 0.620149146921328 1104 74-75 0.6408026886445413 1104 76-77 0.5215580757344327 1104 78-79 0.5808704046288717 1104 80-81 0.7135395507363604 1104 82-83 0.7832689559045072 1104 84-85 0.5503712946254176 1104 86-87 0.505035157979222 1104 88-89 0.6453453634895467 1104 90-91 0.6958818612054181 1104 92-93 0.5895336715590496 1104 94-95 0.38206481203931375 1104 96-97 0.7240984580125165 1104 98-99 0.7714918744645445 1104 100-101 0.9065782607647606 1105 1 0.2938035243350541 1105 2 0.28431142564971523 1105 3 0.25225838136225676 1105 4 0.15590572841497874 1105 5 0.3394774469418067 1105 6 0.276870322720427 1105 7 0.37611110500926515 1105 8 0.40643928644436755 1105 9 0.36474912095479084 1105 10-11 0.43230382779621124 1105 12-13 0.44889367642961275 1105 14-15 0.4136347602561017 1105 16-17 0.5181417949841034 1105 18-19 0.48365487131814433 1105 20-21 0.5061789157884746 1105 22-23 0.5430129284567471 1105 24-25 0.5619474415691315 1105 26-27 0.5464700496002308 1105 28-29 0.6106580559301307 1105 30-31 0.6826395985742124 1105 32-33 0.43633785038138484 1105 34-35 0.3345517691448876 1105 36-37 0.3030403499937151 1105 38-39 0.46970740951795875 1105 40-41 0.34142579893342173 1105 42-43 0.5581441038969288 1105 44-45 0.5398423808949318 1105 46-47 0.4105927069679254 1105 48-49 0.4309024388914082 1105 50-51 0.3104997770403273 1105 52-53 0.37352255083790453 1105 54-55 0.5102898183646261 1105 56-57 0.49175470032563595 1105 58-59 0.5474844422348326 1105 60-61 0.5660371595011071 1105 62-63 0.3615081089026333 1105 64-65 0.4304980978357804 1105 66-67 0.47831913723710073 1105 68-69 0.5982374213612758 1105 70-71 0.6204520155888318 1105 72-73 0.5052978622914921 1105 74-75 0.5887388656923065 1105 76-77 0.7096155401356299 1105 78-79 0.6374822850978852 1105 80-81 0.676375635600845 1105 82-83 0.6920135348992886 1105 84-85 0.6371087748095086 1105 86-87 0.6016823404532268 1105 88-89 0.7228529930524452 1105 90-91 0.7352917834781572 1105 92-93 0.590059360663961 1105 94-95 0.5193724143662415 1105 96-97 0.6085831220673263 1105 98-99 0.6684596906287403 1105 100-101 0.5660253880684678 1106 1 0.19114034115910172 1106 2 0.1983548005616882 1106 3 0.25489517601583245 1106 4 0.3009999066336917 1106 5 0.25773269751188366 1106 6 0.30809056811825286 1106 7 0.2832903777251232 1106 8 0.21163746527624738 1106 9 0.21096936378063447 1106 10-11 0.2986539350735242 1106 12-13 0.2772147336988766 1106 14-15 0.34099636583051307 1106 16-17 0.4550536794383859 1106 18-19 0.4082215054235405 1106 20-21 0.3572841216318139 1106 22-23 0.3532673642233277 1106 24-25 0.2906410245286253 1106 26-27 0.3415644592972171 1106 28-29 0.45344753364829415 1106 30-31 0.3807345838804821 1106 32-33 0.4914196470848182 1106 34-35 0.41188557160750605 1106 36-37 0.44622483251376366 1106 38-39 0.4324532973065587 1106 40-41 0.41503511608797083 1106 42-43 0.42743758593190506 1106 44-45 0.4651492310569907 1106 46-47 0.37571844853427194 1106 48-49 0.37559533667523226 1106 50-51 0.45228870281142264 1106 52-53 0.30701035733441984 1106 54-55 0.2107327648874886 1106 56-57 0.33645233055401746 1106 58-59 0.3740079421656475 1106 60-61 0.44194551879858324 1106 62-63 0.4222762978218171 1106 64-65 0.3365251231805928 1106 66-67 0.36982193015098375 1106 68-69 0.4014135089361872 1106 70-71 0.5482480287100486 1106 72-73 0.5882673889492978 1106 74-75 0.4918816718364525 1106 76-77 0.555591187593194 1106 78-79 0.5159644255208313 1106 80-81 0.5145720701153849 1106 82-83 0.5317984247182039 1106 84-85 0.4628874291183216 1106 86-87 0.35737580561600524 1106 88-89 0.35330539932046534 1106 90-91 0.5030933623656928 1106 92-93 0.4632012974786264 1106 94-95 0.535995897476301 1106 96-97 0.5486194487232829 1106 98-99 0.5104559694870083 1106 100-101 0.5457610090243499 1107 1 0.17736714699213252 1107 2 0.24229125177573962 1107 3 0.2806259003343321 1107 4 0.21075611460658195 1107 5 0.15119101659642808 1107 6 0.2137982107602312 1107 7 0.18387554622081836 1107 8 0.302190503772799 1107 9 0.3259490555049922 1107 10-11 0.3405939194421208 1107 12-13 0.2506604429450263 1107 14-15 0.33001184397082284 1107 16-17 0.07735793105919697 1107 18-19 0.22681490849276997 1107 20-21 0.44405938998630035 1107 22-23 0.3241894695645655 1107 24-25 0.4494709929658498 1107 26-27 0.3908423660640423 1107 28-29 0.5319808654544786 1107 30-31 0.453337691943851 1107 32-33 0.5471740039421533 1107 34-35 0.34035800858421794 1107 36-37 0.4594583452318588 1107 38-39 0.4515774916704203 1107 40-41 0.5173556099660317 1107 42-43 0.1270586362899877 1107 44-45 0.457741496404644 1107 46-47 0.4330953948917369 1107 48-49 0.3219827979580714 1107 50-51 0.3336575029514819 1107 52-53 0.36963849418171435 1107 54-55 0.4592611171164904 1107 56-57 0.37845121901203527 1107 58-59 0.5653008814742506 1107 60-61 0.5234507800791235 1107 62-63 0.6021805677683503 1107 64-65 0.46350461419472566 1107 66-67 0.5213475187964534 1107 68-69 0.5419616660290316 1107 70-71 0.5847222044482194 1107 72-73 0.4411478807329914 1107 74-75 0.4539419841699299 1107 76-77 0.48153865714441935 1107 78-79 0.5354540434023711 1107 80-81 0.5301085691071954 1107 82-83 0.7043083225141586 1107 84-85 0.68865535027847 1107 86-87 0.45652311438087523 1107 88-89 0.6611419884682554 1107 90-91 0.7572036951792498 1107 92-93 0.5674710803897867 1107 94-95 0.4996327123140212 1107 96-97 0.5772231109214196 1107 98-99 0.6741163459604955 1107 100-101 0.6164303721893312 1108 1 0.06442119643764599 1108 2 0.09037060658858564 1108 3 0.03580741041103508 1108 4 -0.03672369834544753 1108 5 0.13100388258856555 1108 6 0.06440984015856799 1108 7 0.13082751662708603 1108 8 0.10113836321185232 1108 9 0.13702936131883092 1108 10-11 0.052725212984150005 1108 12-13 0.05781096556540888 1108 14-15 0.1548978382744508 1108 16-17 0.22240992983891772 1108 18-19 0.17969390183232292 1108 20-21 0.20817690253110044 1108 22-23 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-1.9661193924217244 2316 66-67 -2.0803127615353034 2316 68-69 -2.0955317058557554 2316 70-71 -1.9678718897760703 2316 72-73 -1.836377139359783 2316 74-75 -1.7452412152671322 2316 76-77 -1.9959226246580286 2316 78-79 -2.1634173428158228 2316 80-81 -1.9441301564271392 2316 82-83 -2.054457464072204 2316 84-85 -2.1481456545271236 2316 86-87 -2.1841042304712026 2316 88-89 -2.310227263413463 2316 90-91 -2.3391555520002605 2316 92-93 -2.3960951290619903 2316 94-95 -2.316332457982682 2316 96-97 -2.5822105729099825 2316 98-99 -2.3905525901061218 2316 100-101 -2.0618102759152777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25220.0 3 5648.0 4 4679.0 5 3747.0 6 3817.0 7 3919.0 8 4758.0 9 5639.0 10 6410.0 11 7910.0 12 9422.0 13 13315.0 14 13508.0 15 12038.0 16 13773.0 17 16395.0 18 15458.0 19 12348.0 20 12103.0 21 12754.0 22 13880.0 23 15274.0 24 17121.0 25 20167.0 26 23938.0 27 28546.0 28 34063.0 29 41393.0 30 49997.0 31 62304.0 32 77718.0 33 101848.0 34 140021.0 35 206533.0 36 338821.0 37 555358.0 38 527906.0 39 101588.0 40 75.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.8007883563427 17.887652185062038 12.90632522279041 24.40523423580486 2 15.557086481098171 18.861462917887643 40.492542078417685 25.088908522596498 3 20.387766410145996 22.849356692422937 27.81899857685343 28.94387832057764 4 12.683130395640585 14.884016439242556 36.17621936495259 36.256633800164266 5 14.398708333235646 36.88401023142623 33.66762088079982 15.049660554538299 6 34.55682071716572 34.60785091367887 16.699846714042174 14.135481655113239 7 30.679953299073482 31.02841285619616 19.521471798141967 18.770162046588393 8 27.110874054623284 35.66440616372918 18.723841565510625 18.500878216136904 9 26.75824899404418 16.141209350963596 19.24029966287731 37.860241992114915 10-11 26.07455275361317 25.875401053116693 26.484656000309453 21.56539019296069 12-13 26.2259667610204 22.63457910473889 27.47149068992843 23.667963444312285 14-15 23.066449488108418 24.346876905514236 25.087171323521222 27.49950228285612 16-17 23.33142236790891 27.65528450328186 25.61811329484829 23.395179833960945 18-19 23.18060041749281 26.645919676009328 27.331144791538854 22.842335114959013 20-21 23.842212898572356 25.720034446622975 27.615262948546533 22.822489706258143 22-23 23.43753602274508 25.707745588666846 27.345185720085635 23.509532668502434 24-25 23.22387917380041 25.728130108498 27.602781563116856 23.445209154584738 26-27 23.139158449596653 26.086343219867107 27.405424731508415 23.36907359902782 28-29 23.090501463306357 26.287617759134573 27.457461729136114 23.164419048422953 30-31 23.269060856170046 26.05247125733831 27.382619233052274 23.295848653439368 32-33 23.030322568670034 26.01265890798113 27.464554959961003 23.492463563387837 34-35 23.397327352766908 26.213233961913062 27.335569516739543 23.05386916858048 36-37 23.4119823533314 26.04871793160758 27.22384098057634 23.315458734484682 38-39 23.196034307681863 26.300148646733795 27.259934739883917 23.243882305700428 40-41 23.555425911418027 26.141381512270513 27.175893309505334 23.12729926680613 42-43 23.282062475023416 26.063894517752708 27.181942587420988 23.47210041980289 44-45 23.25725431595826 26.221760415484717 27.23402935452322 23.2869559140338 46-47 23.34353229655326 26.400256790065285 26.858336499036543 23.397874414344905 48-49 23.523426986830184 26.296729398580176 26.9043495060387 23.275494108550934 50-51 23.439304922613953 26.598699390497604 26.716531414771673 23.245464272116774 52-53 23.56251114187862 26.48769112003072 26.611616296867552 23.338181441223107 54-55 23.451071449113847 26.47422285364181 26.78185995297595 23.29284574426839 56-57 23.28250455951395 26.631997806172514 26.786602184051837 23.298895450261696 58-59 23.461282371997964 26.49898751013864 26.719937216991614 23.319792900871786 60-61 23.44907550080066 26.561661480070242 26.732687648860292 23.25657537026881 62-63 23.395414899104697 26.56587735848316 26.87404751866098 23.164660223751167 64-65 23.29124122541216 26.545041068320117 26.754097948714023 23.4096197575537 66-67 23.269032580064536 26.71796491226693 26.589097794755073 23.423904712913462 68-69 23.28922965838482 26.477242766789864 26.85411039353288 23.379417181292432 70-71 23.30619082716109 26.287947412171242 26.76481412601097 23.641047634656697 72-73 23.380898838468344 26.601552163708988 26.52947171627628 23.488077281546385 74-75 23.21815259801579 26.775898781573332 26.545159059827416 23.460789560583457 76-77 23.404564382957155 26.55197539845404 26.58719113113169 23.456269087457112 78-79 23.370890002367332 26.604200028816262 26.571889698893635 23.45302026992277 80-81 23.33004198917586 26.581719887636257 26.480780971188633 23.607457151999245 82-83 23.43971790614054 26.75457202134745 26.515358375844173 23.290351696667834 84-85 23.5114384836135 26.676610073136647 26.315824601607762 23.49612684164209 86-87 23.31900097937425 26.578340419024553 26.577005533297644 23.52565306830355 88-89 23.445363104624644 26.634917132104867 26.445476191972055 23.47424357129843 90-91 23.2529879148027 26.788487445455583 26.447631466287525 23.510893173454196 92-93 23.29041913698521 26.68401534873364 26.49704543553465 23.528520078746496 94-95 23.259099484768708 26.645723965037217 26.551263913571017 23.54391263662306 96-97 23.379061961296564 26.46566584922427 26.514826217607144 23.640445971872026 98-99 23.1148066631397 26.482032097482406 26.545356571538015 23.85780466783988 100-101 23.197698869785853 26.67604472343989 26.229187121473636 23.897069285300624 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 78.0 1 63.5 2 119.5 3 210.5 4 181.0 5 177.0 6 330.5 7 615.0 8 1008.5 9 1254.5 10 1218.5 11 1223.0 12 1389.5 13 1644.5 14 1910.0 15 2129.0 16 2447.5 17 2693.5 18 2824.0 19 3003.5 20 3175.0 21 3475.0 22 3905.5 23 4566.0 24 5825.5 25 7403.0 26 9518.0 27 12322.5 28 15107.5 29 18320.0 30 21871.5 31 26144.0 32 31670.5 33 36284.5 34 40542.5 35 45695.5 36 51376.0 37 56151.5 38 59594.5 39 63642.5 40 68225.5 41 72353.5 42 76046.5 43 80851.5 44 86111.0 45 90570.0 46 93534.0 47 97077.0 48 100462.5 49 101320.5 50 99834.0 51 98084.5 52 97401.0 53 96251.0 54 95684.5 55 91739.5 56 84877.5 57 77706.5 58 73041.5 59 68780.5 60 59674.0 61 49666.5 62 40207.0 63 30548.5 64 22860.5 65 17484.5 66 12433.5 67 8876.0 68 6830.5 69 5308.0 70 3957.5 71 2734.5 72 2029.5 73 1629.0 74 1249.0 75 906.5 76 613.0 77 365.0 78 239.0 79 148.5 80 93.5 81 64.0 82 47.0 83 41.5 84 34.5 85 26.0 86 21.5 87 22.5 88 23.5 89 21.0 90 22.5 91 24.5 92 23.0 93 26.0 94 24.5 95 22.0 96 24.0 97 20.0 98 15.5 99 16.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04602619664204122 2 0.012541943227585088 3 0.011487013423395687 4 0.0066421506189702955 5 0.009299010866558413 6 0.005587220814780895 7 0.00578257818592708 8 0.009650654134621545 9 0.015745804114382523 10-11 0.002207538293951892 12-13 0.009689725608850782 14-15 0.008888760387151425 16-17 0.019379451217701564 18-19 0.012737300598731271 20-21 0.0035164326806313324 22-23 0.010353940670747812 24-25 0.021997239991060447 26-27 0.008087795165452065 28-29 0.01879337910426301 30-31 0.01605837590821642 32-33 0.013460122871972156 34-35 0.26175934159877345 36-37 0.46295789814222954 38-39 0.4595196084100567 40-41 0.4793483815813944 42-43 0.46987354908080453 44-45 0.48491606665906073 46-47 0.49169496743783336 48-49 0.4983566537939183 50-51 0.5075579859749036 52-53 0.4967547233505195 54-55 0.4851700312415508 56-57 0.4792311671587068 58-59 0.4776878439266519 60-61 0.4743667686171667 62-63 0.4734681247098943 64-65 0.48829574917988977 66-67 0.46207878997207175 68-69 0.48825667770566056 70-71 0.47098708609633777 72-73 0.47893813110198746 74-75 0.49241778971107425 76-77 0.47882091667929977 78-79 0.47819577309163197 80-81 0.48163406282380483 82-83 0.48122381234439787 84-85 0.4817708129836072 86-87 0.4836071722723813 88-89 0.4965202945051442 90-91 0.5205101796818956 92-93 0.5084370941450614 94-95 0.48071588317941777 96-97 0.4543231023375682 98-99 0.4463525215948038 100-101 0.4540300662808489 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2559412.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.73304134305874 #Duplication Level Percentage of deduplicated Percentage of total 1 71.7774113280415 37.13263787731304 2 14.492230995090576 14.994543704443561 3 5.634602226656414 8.744853298299214 4 2.723736602275294 5.636287130124405 5 1.508895831985626 3.902988522924071 6 0.9261631012836148 2.874794040547245 7 0.6036407217354363 2.1859719289725197 8 0.43210891688109687 1.7883446769371294 9 0.3108458557282737 1.4472901355138332 >10 1.451284965994671 13.206891855665113 >50 0.09497674327921687 3.403033626367228 >100 0.0425907805905436 3.8034805693034586 >500 0.0011339478432888911 0.42119725732148144 >1k 3.7798261442629604E-4 0.45768537626762273 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4431 0.17312570230974927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3133 0.12241092876019961 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5628589691694811E-4 2 0.0 0.0 0.0 0.0 1.5628589691694811E-4 3 0.0 0.0 0.0 0.0 1.5628589691694811E-4 4 0.0 0.0 0.0 0.0 1.5628589691694811E-4 5 0.0 0.0 0.0 0.0 1.9535737114618514E-4 6 0.0 0.0 0.0 0.0 1.9535737114618514E-4 7 0.0 0.0 0.0 0.0 1.9535737114618514E-4 8 0.0 0.0 0.0 0.0 1.9535737114618514E-4 9 0.0 0.0 0.0 3.907147422923703E-5 1.9535737114618514E-4 10-11 0.0 0.0 0.0 5.8607211343855546E-5 1.9535737114618514E-4 12-13 0.0 0.0 0.0 1.1721442268771108E-4 4.297862165216073E-4 14-15 0.0 0.0 0.0 1.367501598023296E-4 4.493219536362258E-4 16-17 0.0 0.0 0.0 2.3442884537542216E-4 4.688576907508443E-4 18-19 0.0 0.0 0.0 2.3442884537542216E-4 5.079291649800813E-4 20-21 0.0 0.0 0.0 3.9071474229237027E-4 6.44679324782411E-4 22-23 0.0 0.0 0.0 7.032865361262664E-4 7.032865361262666E-4 24-25 0.0 0.0 0.0 9.767868557309257E-4 7.032865361262666E-4 26-27 0.0 0.0 0.0 0.0011135370155332552 7.032865361262666E-4 28-29 0.0 0.0 0.0 0.00144564454648177 7.618937474701221E-4 30-31 0.0 0.0 0.0 0.00289128909296354 7.814294845847405E-4 32-33 0.0 0.0 0.0 0.005587220814780895 8.205009588139776E-4 34-35 0.0 0.0 0.0 0.008752010227349095 8.595724330432146E-4 36-37 0.0 0.0 0.0 0.01578487558861176 8.595724330432146E-4 38-39 0.0 0.0 0.0 0.026881174269715077 8.595724330432146E-4 40-41 0.0 0.0 0.0 0.03973568929113406 8.595724330432146E-4 42-43 0.0 0.0 0.0 0.05245345415275071 8.595724330432146E-4 44-45 0.0 0.0 0.0 0.06698804256602689 9.572511186163072E-4 46-47 0.0 0.0 0.0 0.08601585051566532 0.0010549298041893998 48-49 0.0 0.0 0.0 0.10740748265617259 0.0010940012784186367 50-51 0.0 0.0 0.0 0.13116293898754872 0.0011330727526478739 52-53 0.0 0.0 0.0 0.15521143137564408 0.001250287175335585 54-55 0.0 0.0 0.0 0.1826396062845685 0.001250287175335585 56-57 0.0 0.0 0.0 0.21499078694637674 0.0012698229124502034 58-59 0.0 0.0 0.0 0.2542185470725307 0.0012893586495648218 60-61 0.0 0.0 0.0 0.2953803451730319 0.0012893586495648218 62-63 0.0 0.0 0.0 0.336600750484877 0.001406573072252533 64-65 0.0 0.0 0.0 0.37950122918857926 0.001484716020711007 66-67 0.0 0.0 0.0 0.4297471450473781 0.0015042517578256255 68-69 0.0 0.0 0.0 0.4925740756079912 0.0015237874949402441 70-71 0.0 0.0 0.0 0.5623166571071794 0.0015237874949402441 72-73 0.0 0.0 0.0 0.6332704543074738 0.001601930443398718 74-75 0.0 0.0 0.0 0.7065294684872931 0.0017191448660864293 76-77 0.0 0.0 0.0 0.7871143840850945 0.0017972878145449034 78-79 0.0 0.0 0.0 0.8845195693385824 0.0018168235516595218 80-81 0.0 0.0 0.0 0.9927084814793399 0.0018363592887741403 82-83 0.0 0.0 0.0 1.1084381881463399 0.0019145022372326144 84-85 0.0 0.0 0.0 1.2249297885608101 0.0019145022372326144 86-87 0.0 0.0 0.0 1.3504273637851192 0.0020903238712641813 88-89 0.0 0.0 3.907147422923703E-5 1.49788310752626 0.002227074031066511 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3380 0.0 47.899742 1 GTATCAA 5830 0.0 40.437523 1 ATCAACG 7085 0.0 32.531384 3 TCAACGC 7195 0.0 32.099808 4 CAACGCA 7270 0.0 31.833755 5 TATCAAC 7430 0.0 31.150063 2 AACGCAG 7630 0.0 30.267376 6 GTACATG 9580 0.0 26.93114 1 TACATGG 9325 0.0 26.697819 2 GTACCTG 2405 0.0 24.998411 1 ACATGGG 9745 0.0 24.817183 3 ACGCAGA 9280 0.0 24.58077 7 GTGTAGC 1855 0.0 24.243956 1 CGCAGAG 9385 0.0 24.053625 8 GTATAGG 730 0.0 23.34551 1 TACCTGG 2405 0.0 23.22233 2 CATGGGG 6145 0.0 21.565004 4 GAGTACA 7150 0.0 20.922077 1 AGTACAT 6710 0.0 20.4557 2 TAGCCCT 2120 0.0 20.091856 4 >>END_MODULE