FastQCFastQC Report
Thu 26 May 2016
SRR936338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936338_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559412
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42250.16507697861852647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30300.11838656691458821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35150.043.719361
GTATCAA58400.039.5116771
ATCAACG72300.031.0970783
TCAACGC72800.030.9486544
CAACGCA73650.030.592075
TATCAAC76100.029.7382282
AACGCAG77650.029.0161786
GGACCGA8700.025.625166
TACATGG94000.025.3371182
GTACATG96400.025.3184381
TACCTGG25450.025.1667612
GTACCTG25950.024.5676751
ACGCAGA91850.024.4269927
GTGTACG3150.024.166071
CGCAGAG92500.024.1015038
ACATGGG95900.023.8400963
ACCGAGT10100.023.0124448
CGCGAAA652.89177E-421.98055872-73
AGGACCG10200.021.3917185
CATGGGG58800.020.9737244
Produced by FastQC (version 0.11.5)