##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936338_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2559412 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.493593059655886 34.0 31.0 34.0 31.0 34.0 2 32.63282894664869 34.0 31.0 34.0 31.0 34.0 3 32.719828616885444 34.0 31.0 34.0 31.0 34.0 4 36.19861241566422 37.0 35.0 37.0 35.0 37.0 5 36.160296974461325 37.0 35.0 37.0 35.0 37.0 6 36.192446546316106 37.0 37.0 37.0 35.0 37.0 7 36.14186344363471 37.0 36.0 37.0 35.0 37.0 8 36.11719058908843 37.0 36.0 37.0 35.0 37.0 9 37.89140318166829 39.0 38.0 39.0 35.0 39.0 10-11 37.8253499241232 39.0 38.0 39.0 35.0 39.0 12-13 37.77354388429843 39.0 38.0 39.0 35.0 39.0 14-15 39.13404836735938 40.5 38.5 41.0 36.0 41.0 16-17 39.06240515399631 40.0 38.0 41.0 36.0 41.0 18-19 39.075201843235874 40.0 38.5 41.0 36.0 41.0 20-21 39.01945544523508 40.0 38.5 41.0 35.0 41.0 22-23 38.91104890498286 40.0 38.0 41.0 35.0 41.0 24-25 39.071606095462556 40.0 39.0 41.0 35.5 41.0 26-27 38.997609021134544 40.0 39.0 41.0 35.0 41.0 28-29 38.91612507091473 40.0 38.0 41.0 35.0 41.0 30-31 38.81712537879794 40.0 38.0 41.0 35.0 41.0 32-33 38.67764588116333 40.0 38.0 41.0 35.0 41.0 34-35 38.55285901605525 40.0 38.0 41.0 34.0 41.0 36-37 38.42526213052061 40.0 38.0 41.0 34.0 41.0 38-39 38.29649778933599 40.0 38.0 41.0 34.0 41.0 40-41 38.16700808623231 40.0 38.0 41.0 33.0 41.0 42-43 38.20673713337282 40.0 38.0 41.0 34.0 41.0 44-45 38.09345759885474 40.0 38.0 41.0 33.0 41.0 46-47 37.92328882571466 40.0 38.0 41.0 33.0 41.0 48-49 37.71674646364087 40.0 37.0 41.0 33.0 41.0 50-51 37.530809224931346 40.0 37.0 41.0 33.0 41.0 52-53 37.31943801935757 40.0 37.0 41.0 32.0 41.0 54-55 37.13035337804152 40.0 36.0 41.0 32.0 41.0 56-57 36.875958423262844 39.0 36.0 41.0 31.0 41.0 58-59 36.60711640017317 39.0 35.0 41.0 31.0 41.0 60-61 36.3302362026903 39.0 35.0 40.5 31.0 41.0 62-63 36.02546405189942 38.0 35.0 40.0 30.0 41.0 64-65 35.650055364278984 37.5 35.0 40.0 30.0 41.0 66-67 35.30355116722122 37.0 34.5 40.0 29.0 41.0 68-69 34.94093018240127 36.5 34.0 39.0 29.0 41.0 70-71 34.56783081426515 36.0 34.0 39.0 29.0 40.5 72-73 34.16261078716518 35.0 34.0 38.5 28.5 40.0 74-75 33.731085694682996 35.0 33.5 37.0 28.0 39.5 76-77 33.009528555777656 35.0 32.5 37.0 26.5 39.0 78-79 33.06398539977151 35.0 33.0 36.0 27.0 39.0 80-81 32.89611871789302 35.0 33.0 36.0 27.0 38.0 82-83 32.61741192898994 35.0 33.0 36.0 27.0 37.0 84-85 32.33141791942837 35.0 33.0 35.0 26.0 37.0 86-87 31.93487449461048 35.0 32.5 35.0 25.5 36.0 88-89 31.449843753174555 35.0 32.0 35.0 24.5 36.0 90-91 31.229520686782745 35.0 32.0 35.0 24.0 36.0 92-93 30.94391915017981 34.0 32.0 35.0 23.0 35.0 94-95 30.790046698226 34.0 32.0 35.0 22.5 35.0 96-97 30.540685907544386 34.0 31.0 35.0 19.5 35.0 98-99 30.09543832724079 34.0 31.0 35.0 9.5 35.0 100-101 26.73691359577903 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1428369453056746 1101 2 0.09294411168025363 1101 3 0.22097631162392872 1101 4 0.18787456264326607 1101 5 0.15808996010975562 1101 6 0.19806615491546609 1101 7 0.18245640440609634 1101 8 0.12680891774751757 1101 9 0.16683608785604775 1101 10-11 0.24053131834958918 1101 12-13 0.33768625714102996 1101 14-15 0.3939919287373428 1101 16-17 0.3853961872639786 1101 18-19 0.39205714360888777 1101 20-21 0.3208997016701929 1101 22-23 0.30523297587753717 1101 24-25 0.20032815822290928 1101 26-27 0.3565066799869072 1101 28-29 0.340849736432439 1101 30-31 0.3259633281349039 1101 32-33 0.44579438420417006 1101 34-35 0.45974308606195535 1101 36-37 0.2801852800826907 1101 38-39 0.30131495967156496 1101 40-41 0.35704901830919056 1101 42-43 0.2852264365649617 1101 44-45 0.42534982270645827 1101 46-47 0.4624845282909362 1101 48-49 0.46526282200494506 1101 50-51 0.3534049889576849 1101 52-53 0.5367269011444407 1101 54-55 0.4513679466475651 1101 56-57 0.2915623105190619 1101 58-59 0.3072528224742044 1101 60-61 0.48713401511698606 1101 62-63 0.5599676121543737 1101 64-65 0.5731347768696722 1101 66-67 0.4969629532878912 1101 68-69 0.7166899123524004 1101 70-71 0.6505623539770582 1101 72-73 0.5254518656292433 1101 74-75 0.5689101439103581 1101 76-77 0.39326107526601817 1101 78-79 0.35038776471927946 1101 80-81 0.4741943067022589 1101 82-83 0.5573973797560328 1101 84-85 0.6255772854636703 1101 86-87 0.6242595136749785 1101 88-89 0.6528515873230276 1101 90-91 0.8307318621881414 1101 92-93 0.8630695925900262 1101 94-95 0.7003983250550085 1101 96-97 0.5755178768930911 1101 98-99 0.6476912832259281 1101 100-101 1.1104365574065653 1102 1 0.0697876127249657 1102 2 0.11596308797644639 1102 3 0.11401782850880693 1102 4 0.13043091221054226 1102 5 0.05958705436201939 1102 6 0.108325245994223 1102 7 0.04991046814896549 1102 8 0.10422376589372817 1102 9 0.08305959847464806 1102 10-11 0.10329044180330271 1102 12-13 0.22338132464472693 1102 14-15 0.29427220065652904 1102 16-17 0.25399851460451117 1102 18-19 0.3206851195341116 1102 20-21 0.24685379169390842 1102 22-23 0.2730326518232502 1102 24-25 0.16313075934132826 1102 26-27 0.3010146682222299 1102 28-29 0.19627259909481864 1102 30-31 0.22476853981761735 1102 32-33 0.2225893280736173 1102 34-35 0.27097422574428975 1102 36-37 0.16613763328510345 1102 38-39 0.19944772442444503 1102 40-41 0.20494803586250754 1102 42-43 0.2837025282295187 1102 44-45 0.3066421815306839 1102 46-47 0.39369764464348833 1102 48-49 0.3224006052572719 1102 50-51 0.15301589909013558 1102 52-53 0.12680888977435245 1102 54-55 0.09392621966825487 1102 56-57 0.3547109047221966 1102 58-59 0.13371485655094517 1102 60-61 0.23607659184373375 1102 62-63 0.28873297216772187 1102 64-65 0.2469713541738443 1102 66-67 0.2290975942648359 1102 68-69 0.25649054354982326 1102 70-71 0.20457692499362423 1102 72-73 0.03842089307787688 1102 74-75 0.0926177546155813 1102 76-77 0.06302155008858534 1102 78-79 0.14651770028168443 1102 80-81 0.16641881294606264 1102 82-83 0.17249721270125207 1102 84-85 0.12229141762814777 1102 86-87 0.17274339437364006 1102 88-89 0.3259757251017099 1102 90-91 0.3999515228528665 1102 92-93 0.35622714513458575 1102 94-95 0.25125376667610055 1102 96-97 0.1497785090198711 1102 98-99 0.2700219679067821 1102 100-101 0.7068551823773319 1103 1 0.031820216481179386 1103 2 0.01742832119996507 1103 3 0.10960770372032158 1103 4 -0.033636679557119464 1103 5 0.04785319255614695 1103 6 0.03078986334741529 1103 7 0.0874908900500273 1103 8 0.11596715724643047 1103 9 0.10904723323365317 1103 10-11 0.11122023931351777 1103 12-13 0.12188763357843158 1103 14-15 0.16503108518382703 1103 16-17 0.12144541447476342 1103 18-19 0.08044991537010304 1103 20-21 0.06217706008182233 1103 22-23 0.08495106918601181 1103 24-25 0.126332694549383 1103 26-27 0.11477583834594185 1103 28-29 0.12422034952383854 1103 30-31 0.10650247262795887 1103 32-33 0.022190993016245386 1103 34-35 0.07153940261267877 1103 36-37 0.22794169608594217 1103 38-39 0.1100156368333387 1103 40-41 0.18790836529333177 1103 42-43 0.13416310430744005 1103 44-45 0.041875961868278466 1103 46-47 0.06885996459110544 1103 48-49 0.08837193888557948 1103 50-51 0.08757528647853974 1103 52-53 0.04523512809225139 1103 54-55 0.06997062062973214 1103 56-57 0.012182648681580588 1103 58-59 0.09741597184426354 1103 60-61 0.004296898523236337 1103 62-63 -0.013432794859895125 1103 64-65 -0.046480950257432596 1103 66-67 -0.07050294955463698 1103 68-69 -0.065871229439189 1103 70-71 -0.15396881757855851 1103 72-73 -0.21655577261217474 1103 74-75 -0.05901022831943692 1103 76-77 -0.14742167473995949 1103 78-79 -0.2284144717673655 1103 80-81 -0.14566833345169528 1103 82-83 -0.14616578814183612 1103 84-85 -0.060326558161570176 1103 86-87 -0.17191733133685716 1103 88-89 -0.11538710473272218 1103 90-91 0.07597215600343077 1103 92-93 0.24887698698147886 1103 94-95 0.28176307797762234 1103 96-97 0.17428981143611466 1103 98-99 0.15243343281114363 1103 100-101 0.510529420611519 1104 1 -0.05622685804866023 1104 2 0.11324907810732299 1104 3 0.04591197583548734 1104 4 0.029837026038421754 1104 5 0.06045041800309292 1104 6 0.04914951043355131 1104 7 -0.01200702032842571 1104 8 0.041425713085764926 1104 9 0.06275631147837402 1104 10-11 0.05323541809224963 1104 12-13 0.12956626719812192 1104 14-15 0.06163472460801955 1104 16-17 0.15750662297255502 1104 18-19 0.23015243452497458 1104 20-21 0.09058793191837111 1104 22-23 0.08295504527145425 1104 24-25 0.15516221625822624 1104 26-27 0.2039598701546268 1104 28-29 0.1516052752261814 1104 30-31 0.15665556642874634 1104 32-33 0.03061187762269668 1104 34-35 0.0558969890997858 1104 36-37 -0.019706754111410874 1104 38-39 0.13995599555170912 1104 40-41 0.12845065110712994 1104 42-43 0.0345512805321917 1104 44-45 0.14451338350746568 1104 46-47 0.0636163205233089 1104 48-49 0.025313255347157337 1104 50-51 0.03082682467218234 1104 52-53 -0.019922609311976203 1104 54-55 -0.0523897810419669 1104 56-57 -0.05135853427638892 1104 58-59 0.13061410859837963 1104 60-61 -0.06869909636880323 1104 62-63 -1.58619099508428E-4 1104 64-65 -0.08837060663078233 1104 66-67 -0.17180460964286226 1104 68-69 -0.03541209314942506 1104 70-71 0.04491744315976831 1104 72-73 -0.1162510361366671 1104 74-75 -0.06111581225256657 1104 76-77 -0.04599521780937721 1104 78-79 -0.040074269770620674 1104 80-81 -0.010878311394577622 1104 82-83 0.07173094972674932 1104 84-85 0.12320388646346458 1104 86-87 -0.012056929665156702 1104 88-89 0.13286719060045726 1104 90-91 0.21162487251143958 1104 92-93 0.16581162826311413 1104 94-95 0.11854767282321887 1104 96-97 0.12449442021252466 1104 98-99 0.018601153962755745 1104 100-101 0.1437565798871141 1105 1 -0.032250476521632265 1105 2 0.046621868676695044 1105 3 0.07209062981996794 1105 4 0.04467696234815577 1105 5 0.07340566022862305 1105 6 0.08867885195164149 1105 7 0.15339579265824455 1105 8 0.0651066891111256 1105 9 0.11319435663421018 1105 10-11 0.1128199473639242 1105 12-13 0.11778557610288942 1105 14-15 0.1057002515541754 1105 16-17 0.20671023278588052 1105 18-19 0.15052293098778335 1105 20-21 0.18298839842162096 1105 22-23 0.06505053876625055 1105 24-25 0.1074552752764717 1105 26-27 0.20151980674370407 1105 28-29 0.15133178071178577 1105 30-31 0.13541124931827397 1105 32-33 0.14417748488003923 1105 34-35 0.11379210268774642 1105 36-37 0.21558966400958468 1105 38-39 0.1571072354866203 1105 40-41 0.1749527496734018 1105 42-43 0.13908798972887126 1105 44-45 0.14228134353344757 1105 46-47 0.18075865639168143 1105 48-49 0.11839043090741086 1105 50-51 0.07027341074831384 1105 52-53 0.06906812347524749 1105 54-55 0.08192605492767058 1105 56-57 0.16634065492978323 1105 58-59 0.19319834805143188 1105 60-61 0.2810339562245545 1105 62-63 0.19647590563284467 1105 64-65 0.09268738852261293 1105 66-67 0.06319186364842722 1105 68-69 0.14840057576595456 1105 70-71 0.0356482225973167 1105 72-73 0.08741080487511255 1105 74-75 0.18143244429628425 1105 76-77 0.10777757485507067 1105 78-79 -0.09297734464158935 1105 80-81 0.04788346466989424 1105 82-83 0.0711378690593989 1105 84-85 0.1658614360643469 1105 86-87 0.21023518656957663 1105 88-89 0.3046031518555452 1105 90-91 0.46027337488236597 1105 92-93 0.430958492984157 1105 94-95 0.28122846029188153 1105 96-97 0.10098157391417217 1105 98-99 0.05372926776720277 1105 100-101 0.39995677822083664 1106 1 0.005308158209267333 1106 2 0.011900128700858659 1106 3 -0.004304914069081178 1106 4 -0.011052716303424859 1106 5 -0.05356737049574889 1106 6 -0.006593487372860807 1106 7 0.05451805073387561 1106 8 0.054576999965860296 1106 9 -0.02607068443774807 1106 10-11 0.0628335017024213 1106 12-13 -0.03794477126368889 1106 14-15 0.06058642340260434 1106 16-17 -0.047579445289571254 1106 18-19 0.10469846455804799 1106 20-21 0.11051641114983823 1106 22-23 0.08449831392574936 1106 24-25 0.1397104857236542 1106 26-27 -0.07829395098702463 1106 28-29 -0.06547127809346165 1106 30-31 -0.00573210928734369 1106 32-33 -0.01851315676228893 1106 34-35 0.10731276881018204 1106 36-37 0.10599910791134448 1106 38-39 0.10715756914967756 1106 40-41 -0.016054628310548935 1106 42-43 0.00538099903378253 1106 44-45 0.07244589371716614 1106 46-47 0.184529180154982 1106 48-49 0.08927638053671672 1106 50-51 0.0149305579998682 1106 52-53 -0.06811695677312457 1106 54-55 -0.056626804677677 1106 56-57 0.12208638274117334 1106 58-59 0.08102655208357845 1106 60-61 0.07542107121832231 1106 62-63 -0.017335682374934436 1106 64-65 0.048242789759328275 1106 66-67 0.06081175267260619 1106 68-69 0.22299856231655468 1106 70-71 0.1008497347162276 1106 72-73 0.05852025963063312 1106 74-75 0.05814480477275907 1106 76-77 0.06273319018345802 1106 78-79 -0.10867954425096826 1106 80-81 -0.010776214439502496 1106 82-83 0.03950199157416279 1106 84-85 0.24588501598375956 1106 86-87 0.13986527915573532 1106 88-89 0.19087016357914877 1106 90-91 0.3587680974350924 1106 92-93 0.3200155894133694 1106 94-95 0.3112640513613485 1106 96-97 0.2679635654194321 1106 98-99 0.13507561738066087 1106 100-101 0.45215409852554345 1107 1 -0.05247457187528681 1107 2 0.02095172377451604 1107 3 0.10296597765702131 1107 4 0.024130478141699996 1107 5 0.06590539427131858 1107 6 0.07233908326159622 1107 7 -0.011072743647766004 1107 8 0.05287805331278861 1107 9 0.05683354896643067 1107 10-11 0.10673641030999903 1107 12-13 0.11788817892036008 1107 14-15 0.19614409514211673 1107 16-17 0.23681767953637234 1107 18-19 0.19282607263205875 1107 20-21 0.1256539440175004 1107 22-23 0.07683069224925987 1107 24-25 0.09104125454746281 1107 26-27 0.15952258349995674 1107 28-29 0.20337543983066553 1107 30-31 0.06077687985848712 1107 32-33 0.11919396883268263 1107 34-35 0.0440994089263711 1107 36-37 0.04324798150698683 1107 38-39 0.11558603531446465 1107 40-41 0.0038860421816124813 1107 42-43 -0.0601094402325586 1107 44-45 -0.004041884502392179 1107 46-47 0.0792288483831527 1107 48-49 -0.012409530859542883 1107 50-51 0.07871114244842659 1107 52-53 0.10821379841547696 1107 54-55 0.1151088861282119 1107 56-57 0.0014881680804990083 1107 58-59 -0.02224758809398253 1107 60-61 0.021639183244083426 1107 62-63 0.11689392031152579 1107 64-65 0.10492979469908903 1107 66-67 -0.0034907874571672437 1107 68-69 0.046768434947367155 1107 70-71 0.2070246285934232 1107 72-73 0.07880971868929265 1107 74-75 -0.0198000315474971 1107 76-77 -0.057475602997193675 1107 78-79 -0.04201020508394038 1107 80-81 0.14617291369816598 1107 82-83 0.319119158625611 1107 84-85 0.14607367269236704 1107 86-87 0.22593260762075573 1107 88-89 0.3022103461491028 1107 90-91 0.07090081271180892 1107 92-93 0.46091662563447855 1107 94-95 0.5370210643567894 1107 96-97 0.17944680856951223 1107 98-99 0.06174929799723472 1107 100-101 0.16425272578755212 1108 1 -0.149428839343841 1108 2 -0.042112701854094325 1108 3 -0.014807244997484759 1108 4 0.018138672889911334 1108 5 -0.09101252283174688 1108 6 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-1.0327392913056173 2316 62-63 -0.9872418985233224 2316 64-65 -0.8909803664099698 2316 66-67 -0.8489448175179604 2316 68-69 -0.899444201658504 2316 70-71 -0.860337656680656 2316 72-73 -0.9359538868719923 2316 74-75 -0.9095071522275546 2316 76-77 -0.8303086261847668 2316 78-79 -1.0101393924686803 2316 80-81 -1.051303441855616 2316 82-83 -1.1496786763568316 2316 84-85 -0.9735669333924939 2316 86-87 -1.1167513705156367 2316 88-89 -0.9777287252144404 2316 90-91 -1.1287371838132216 2316 92-93 -1.043580488008935 2316 94-95 -0.9717912024085891 2316 96-97 -1.0365063560845407 2316 98-99 -1.2108258956771287 2316 100-101 -1.155498390570468 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 16.0 5 24.0 6 69.0 7 149.0 8 347.0 9 724.0 10 1158.0 11 1828.0 12 2647.0 13 4072.0 14 5308.0 15 6747.0 16 8750.0 17 11656.0 18 11067.0 19 7640.0 20 7133.0 21 7622.0 22 8368.0 23 9617.0 24 11502.0 25 14200.0 26 17660.0 27 22284.0 28 28375.0 29 35640.0 30 44573.0 31 55924.0 32 70300.0 33 91837.0 34 127255.0 35 189487.0 36 325886.0 37 613511.0 38 681558.0 39 134402.0 40 73.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.201708259276515 18.246478081465703 13.327015733018577 24.22479792623921 2 15.828230209192268 19.185115421139635 40.061120251358986 24.925534118309113 3 20.35889493368008 22.848372985670146 28.21749683130344 28.57523524934634 4 13.025218292326517 15.176571806336767 35.99932328206635 35.79888661927036 5 14.302660142251423 36.97087456025056 33.79178498811446 14.93468030938356 6 34.6123640898769 34.8740257527901 16.4782379702838 14.035372187049214 7 30.666379621569327 30.948827308772486 19.512098872709828 18.872694196948363 8 27.05976997841692 35.91649175670037 18.610172961602117 18.413565303280595 9 26.615371030533574 16.06388498608274 19.277396527014798 38.043347456368885 10-11 26.05082040552394 25.9396617573225 26.502487882925912 21.507029954227644 12-13 26.139877186515093 22.68089573005023 27.53932108167919 23.639906001755485 14-15 22.941441237284188 24.401190585962713 25.181701890903067 27.475666285850032 16-17 23.25229477701601 27.707090609943975 25.649263752935745 23.391350860104268 18-19 23.134805963244684 26.707442959554772 27.3840827502567 22.773668326943845 20-21 23.77887968017654 25.758357779052375 27.61358858987924 22.849173950891846 22-23 23.478652094546863 25.70477235360677 27.37551027425092 23.441065277595445 24-25 23.260050145721106 25.746842644258816 27.624787005717486 23.36832020430259 26-27 23.0967072662075 26.1309572068662 27.455904288104684 23.316431238821615 28-29 23.061820104178654 26.281598067637002 27.497764572185908 23.158817255998436 30-31 23.236876303539375 26.054240522501953 27.40798740944089 23.300895764517783 32-33 23.05497658311816 25.972763235036417 27.468560152365534 23.503700029479894 34-35 23.330647122775453 26.18689969299055 27.408822687636693 23.073630496597303 36-37 23.389311718460167 26.042715695665073 27.335142213412034 23.232830372462722 38-39 23.22771062141897 26.292969645134807 27.276224174452807 23.203095558993418 40-41 23.536232076585243 26.073998303897035 27.203932645840535 23.185836973677194 42-43 23.2366023154256 26.120422527369158 27.144779254280493 23.49819590292475 44-45 23.2388721232663 26.223667406516103 27.25216948588962 23.28529098432797 46-47 23.37541529018145 26.39916938056016 26.871941740971465 23.35347358828692 48-49 23.42995721257898 26.333460529146308 26.97283929776188 23.263742960512833 50-51 23.493406151783645 26.55892380613553 26.718521874547598 23.229148167533232 52-53 23.60163502878415 26.424114994367425 26.65686159968209 23.317388377166335 54-55 23.49360837148514 26.434138436607764 26.79326522498904 23.27898796691806 56-57 23.28451464326266 26.580293935844246 26.830710645816453 23.30448077507664 58-59 23.469608631472177 26.440193672056257 26.781389853315922 23.30880784315564 60-61 23.460602537022666 26.52847684085743 26.760271440951666 23.250649181168235 62-63 23.427099661953605 26.470064217875045 26.898053146582107 23.204782973589246 64-65 23.268693569306272 26.490301691086877 26.804400033679908 23.436604705926943 66-67 23.2568307206559 26.66335600977022 26.68263778606918 23.397175483504704 68-69 23.332175515313676 26.432145352135567 26.920148065258736 23.31553106729202 70-71 23.34724996029356 26.241531978970922 26.791639552654452 23.619578508081066 72-73 23.398216589577263 26.57697257836511 26.553959066425044 23.470851765632585 74-75 23.188587847521227 26.716546613050184 26.583977100990385 23.510888438438204 76-77 23.36228297312168 26.491435582043955 26.6251215749815 23.52115986985287 78-79 23.385045614103706 26.563522406156192 26.653225254470964 23.398206725269137 80-81 23.32836012464735 26.541403280989286 26.540485090044253 23.58975150431911 82-83 23.479455437420782 26.694178194053947 26.49110420674749 23.335262161777784 84-85 23.50805904135454 26.6387824454436 26.35492602248385 23.498232490718014 86-87 23.292490076056673 26.53522107484692 26.643267121552782 23.529021727543626 88-89 23.4157491396557 26.679722402514784 26.451364927663874 23.453163530165646 90-91 23.272447221911946 26.721419095154392 26.49607973395885 23.510053948974814 92-93 23.329506112124516 26.66761367532755 26.49321386655799 23.50966634598994 94-95 23.240873767706134 26.6269639240855 26.525141315012036 23.60702099319633 96-97 23.33440522384473 26.446749378062556 26.586570527334967 23.632274870757747 98-99 23.106738531731015 26.505670323933945 26.526671885473306 23.86091925886173 100-101 23.052524296254994 26.68597851094975 26.48632206850326 23.775175124291998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 80.0 1 60.5 2 91.5 3 155.0 4 129.0 5 105.0 6 242.0 7 537.0 8 821.0 9 921.0 10 851.0 11 775.0 12 862.0 13 1004.5 14 1110.5 15 1243.5 16 1363.0 17 1511.5 18 1724.0 19 1927.5 20 2240.5 21 2621.0 22 3102.0 23 3913.0 24 5168.5 25 6707.5 26 8927.0 27 11821.5 28 14523.5 29 17799.0 30 21618.0 31 26013.0 32 31646.0 33 36454.5 34 40907.5 35 45998.5 36 51611.5 37 56814.5 38 60542.5 39 64362.5 40 68853.0 41 73136.5 42 77294.0 43 82792.5 44 87983.5 45 91654.0 46 95024.0 47 99606.5 48 102981.5 49 103122.0 50 100835.0 51 98990.0 52 98366.0 53 97025.0 54 96558.0 55 92347.0 56 84917.0 57 78176.0 58 73500.0 59 68449.5 60 59086.0 61 48959.5 62 39321.5 63 29689.5 64 21876.5 65 16238.5 66 11526.5 67 8434.5 68 6495.5 69 5012.0 70 3752.0 71 2558.0 72 1839.5 73 1519.0 74 1179.5 75 808.5 76 548.0 77 304.0 78 169.0 79 97.0 80 56.5 81 31.0 82 14.5 83 5.5 84 6.5 85 3.5 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2639278084184961 2 0.02039530954766173 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.7350031960465923E-4 12-13 9.767868557309257E-5 14-15 0.0 16-17 5.079291649800813E-4 18-19 0.0 20-21 0.0 22-23 1.5628589691694811E-4 24-25 0.002774074670275829 26-27 0.0025201100877857885 28-29 0.002285681242410366 30-31 0.001328430123794059 32-33 0.0015823947062840996 34-35 0.0017386806032010477 36-37 5.860721134385554E-5 38-39 1.3675015980232962E-4 40-41 6.056078505531739E-4 42-43 0.003848540211579847 44-45 0.00420018347964298 46-47 0.013987587774066855 48-49 0.014280623830786134 50-51 0.006661686356084913 52-53 0.007462651577784272 54-55 3.7117900517775175E-4 56-57 0.0030475749898804883 58-59 0.001328430123794059 60-61 0.001328430123794059 62-63 0.0 64-65 8.791081701578331E-4 66-67 1.9535737114618514E-5 68-69 0.0 70-71 1.3675015980232962E-4 72-73 0.0017972878145449034 74-75 0.0 76-77 0.19217304599650234 78-79 0.10303147754249803 80-81 0.0012307514382209664 82-83 0.0 84-85 7.618937474701221E-4 86-87 0.12090667700237398 88-89 0.5835910748249988 90-91 0.30971957621516194 92-93 0.29370027178117475 94-95 0.0023052169795249847 96-97 0.002774074670275829 98-99 0.0030671107269951065 100-101 6.056078505531739E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2559412.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.78774023254375 #Duplication Level Percentage of deduplicated Percentage of total 1 69.87447405966607 34.78892163368271 2 15.515852589511434 15.44998476426073 3 5.971031266582488 8.918524608630166 4 2.8902867981875344 5.756033932228463 5 1.585380414944516 3.9466254134509993 6 0.994622522982642 2.9712004682218227 7 0.6375464833195841 2.221939908838107 8 0.4408974379882711 1.7561029689403287 9 0.34293153026429907 1.5366407351712874 >10 1.59369105664149 14.058086806831646 >50 0.105505248166254 3.642866063375054 >100 0.045742041892257126 3.903226197634747 >500 0.001646521035201063 0.570478795736013 >1k 3.920288179030094E-4 0.47936770299790815 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4225 0.16507697861852647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3030 0.11838656691458821 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1721442268771108E-4 2 0.0 0.0 0.0 0.0 1.1721442268771108E-4 3 0.0 0.0 0.0 0.0 1.1721442268771108E-4 4 0.0 0.0 0.0 0.0 1.1721442268771108E-4 5 0.0 0.0 0.0 0.0 1.9535737114618514E-4 6 0.0 0.0 0.0 0.0 2.3442884537542216E-4 7 0.0 0.0 0.0 0.0 2.3442884537542216E-4 8 0.0 0.0 0.0 0.0 2.3442884537542216E-4 9 0.0 0.0 0.0 3.907147422923703E-5 2.3442884537542216E-4 10-11 0.0 0.0 0.0 3.907147422923703E-5 2.3442884537542216E-4 12-13 0.0 0.0 0.0 7.814294845847406E-5 5.274649020946999E-4 14-15 0.0 0.0 0.0 9.767868557309257E-5 6.44679324782411E-4 16-17 0.0 0.0 0.0 1.9535737114618514E-4 6.642150618970295E-4 18-19 0.0 0.0 0.0 1.9535737114618514E-4 8.205009588139776E-4 20-21 0.0 0.0 0.0 3.7117900517775175E-4 8.986439072724517E-4 22-23 0.0 0.0 0.0 7.032865361262664E-4 9.767868557309257E-4 24-25 0.0 0.0 0.0 9.767868557309257E-4 9.767868557309257E-4 26-27 0.0 0.0 0.0 0.0011135370155332552 0.0010353940670747813 28-29 0.0 0.0 0.0 0.0014065730722525329 0.0010940012784186367 30-31 0.0 0.0 0.0 0.002852217618734303 0.0010940012784186367 32-33 0.0 0.0 0.0 0.005509077866322421 0.001211215701106348 34-35 0.0 0.0 0.0 0.008791081701578331 0.001250287175335585 36-37 0.0 0.0 0.0 0.015843482799955615 0.001250287175335585 38-39 0.0 0.0 0.0 0.027115603115090498 0.001250287175335585 40-41 0.0 0.0 0.0 0.04030222566745799 0.001250287175335585 42-43 0.0 0.0 0.0 0.05303952626618927 0.0012698229124502034 44-45 0.0 0.0 0.0 0.06773040057638238 0.001328430123794059 46-47 0.0 0.0 0.0 0.0871684590054278 0.0013870373351379146 48-49 0.0 0.0 0.0 0.10881405572842512 0.001562858969169481 50-51 0.0 0.0 0.0 0.13272579795671818 0.0016410019176279552 52-53 0.0 0.0 0.0 0.15681336181904282 0.0016800733918571921 54-55 0.0 0.0 0.0 0.18430014393931105 0.0016800733918571921 56-57 0.0 0.0 0.0 0.21706157508052631 0.0016996091289718106 58-59 0.0 0.0 0.0 0.2562893352066803 0.0017386806032010477 60-61 0.0 0.0 0.0 0.29776370510101535 0.0017582163403156662 62-63 0.0 0.0 0.0 0.339570182526299 0.0020707881341495626 64-65 0.0 0.0 0.0 0.38278323302383516 0.0020707881341495626 66-67 0.0 0.0 0.0 0.4329900774084048 0.0021098596083787995 68-69 0.0 0.0 0.0 0.4957193292834448 0.0021489310826080365 70-71 0.0 0.0 0.0 0.5659112327362692 0.002207538293951892 72-73 0.0 0.0 0.0 0.6368064227252197 0.0023442884537542216 74-75 0.0 0.0 0.0 0.7106319732813631 0.0024810386135565516 76-77 0.0 0.0 0.0 0.7918029609926029 0.00250057435067117 78-79 0.0 0.0 0.0 0.8887002170811108 0.0025396458249004068 80-81 0.0 0.0 0.0 0.9973775226497337 0.0026373245104734993 82-83 0.0 0.0 0.0 1.113341658162109 0.0029108248300781583 84-85 0.0 0.0 0.0 1.230009080210611 0.002930360567192777 86-87 0.0 0.0 0.0 1.356483442290651 0.0030475749898804883 88-89 0.0 0.0 0.0 1.5045252581452302 0.003164789412568199 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3515 0.0 43.71936 1 GTATCAA 5840 0.0 39.511677 1 ATCAACG 7230 0.0 31.097078 3 TCAACGC 7280 0.0 30.948654 4 CAACGCA 7365 0.0 30.59207 5 TATCAAC 7610 0.0 29.738228 2 AACGCAG 7765 0.0 29.016178 6 GGACCGA 870 0.0 25.62516 6 TACATGG 9400 0.0 25.337118 2 GTACATG 9640 0.0 25.318438 1 TACCTGG 2545 0.0 25.166761 2 GTACCTG 2595 0.0 24.567675 1 ACGCAGA 9185 0.0 24.426992 7 GTGTACG 315 0.0 24.16607 1 CGCAGAG 9250 0.0 24.101503 8 ACATGGG 9590 0.0 23.840096 3 ACCGAGT 1010 0.0 23.012444 8 CGCGAAA 65 2.89177E-4 21.980558 72-73 AGGACCG 1020 0.0 21.391718 5 CATGGGG 5880 0.0 20.973724 4 >>END_MODULE