FastQCFastQC Report
Thu 26 May 2016
SRR936337_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936337_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36821
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14253.8700741424730456No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7892.142798946253497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5731.5561771814996876No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5591.5181554004508295No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG4951.3443415442274789No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4581.2438554085983542No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4351.1813910540180874No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4271.1596643219901688No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.9885663072703077No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.9532603677249396No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2970.8066049265364873No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.681676217375954No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC2500.6789603758724642No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2120.5757583987398496No Hit
CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC1890.5132940441595828No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1670.45354553108280604No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1650.4481138480758263No Hit
TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC1650.4481138480758263No Hit
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA1590.4318187990548871No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1540.4182395915374379No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.415523750033948No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.41009206702696827No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1470.39922870101300895No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1360.36935444447462046No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1290.35034355395019146No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.33404850492925237No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1220.3313326634257625No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.2987425653838842No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.29059504087341465No Hit
GCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTA1020.27701583335596536No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1020.27701583335596536No Hit
GTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGC930.25257325982455664No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC870.2362782108036175No Hit
CATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAG830.22541484478965806No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA800.21726732027918852No Hit
ACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGT790.21455147877569866No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.20911979576871895No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT760.2064039542652291No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC730.19825642975475952No Hit
CTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGAT720.19554058825126966No Hit
TATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCA690.1873930637408001No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG670.18196138073382037No Hit
GGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAG670.18196138073382037No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC660.17924553923033051No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT650.17652969772684066No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA640.1738138562233508No Hit
TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.17109801471986094No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG620.1683821732163711No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT600.1629504902093914No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT580.15751880720241165No Hit
GTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATA580.15751880720241165No Hit
ACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTT580.15751880720241165No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.15751880720241165No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC560.15208712419543194No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC540.14665544118845225No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG530.1439395996849624No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG530.1439395996849624No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.14122375818147254No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG510.13850791667798268No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG510.13850791667798268No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA510.13850791667798268No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC500.13579207517449282No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT470.12764455066402325No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC450.12221286765704353No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA450.12221286765704353No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG450.12221286765704353No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT430.11678118465006382No Hit
AAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGT410.1113495016430841No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG400.10863366013959426No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC400.10863366013959426No Hit
TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.10863366013959426No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA380.10320197713261454No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC370.10048613562912469No Hit
GTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACT370.10048613562912469No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG370.10048613562912469No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTATA156.642269E-494.7269061
CAGTCCC200.002081746771.045179
AGTACTC200.002081746771.045175
GCAGTCC200.002081746771.045178
CGCAGAA551.1677912E-860.2807549
CAGAAAA603.79805E-935.52258710-11
TAATGGG650.00585359329.1467382
GTAATGG650.00585359329.1467381
GAGTAAT500.001666730728.4180721
GCAGAAA605.41231E-627.6286810-11
GAGTACT4400.026.9110512-13
TGGGCTT2059.131327E-1025.4145366
GGGCTTG1901.1479642E-824.9281317
TGGTCGC1001.9630534E-823.7786594-95
GGCTTGA2001.9801519E-823.6817268
ACTTTTT5350.023.01775216-17
GTACTTT5100.022.7530314-15
ATGGGCT2303.4597178E-922.6520845
CATGGGG650.007623305521.8600544
AGTACTT4400.020.45239812-13
Produced by FastQC (version 0.11.5)