FastQCFastQC Report
Thu 26 May 2016
SRR936337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936337_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36821
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14774.011297900654518No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8092.197115776323294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6201.6838217321637108No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5651.5344504494717688No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4921.3361940197170095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4841.3144672876890906No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4651.262866299122783No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG4181.13522174845876No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.9831346242633279No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.8527742320958148No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC2780.7550039379701801No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2750.7468564134597104No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.7332772059422612No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT2370.643654436327096No Hit
CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC2210.6002009722712582No Hit
TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC1930.5241574101735422No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1930.5241574101735422No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1840.49971483664213356No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1810.49156731213166394No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1700.46169305559327556No Hit
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA1690.45897721408978576No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1630.4426821650688466No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1610.43725048206186684No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1410.38293365199206975No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1280.3476277124467016No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT1240.3367643464327422TruSeq Adapter, Index 10 (95% over 21bp)
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.32861682192227265No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.3259009804187828No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.3150376144048233No Hit
GCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTA1070.29059504087341465No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1050.28516335786643493No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1040.2824475163629451No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.2607207843350262No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.2552891013280465No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG930.25257325982455664TruSeq Adapter, Index 10 (96% over 28bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA870.2362782108036175No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC840.22813068629314792No Hit
CATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAG830.22541484478965806No Hit
CTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGAT810.21998316178267838No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC810.21998316178267838No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG790.21455147877569866No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.2118356372722088No Hit
TATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCA700.19010890524428994No Hit
ACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTT700.19010890524428994No Hit
ACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGT690.1873930637408001No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA690.1873930637408001No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT680.18467722223731023No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC640.1738138562233508No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT620.1683821732163711No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT620.1683821732163711No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT570.1548029656989218No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC570.1548029656989218No Hit
GTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATA570.1548029656989218No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA570.1548029656989218No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT570.1548029656989218RNA PCR Primer, Index 10 (95% over 23bp)
TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.15208712419543194No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC560.15208712419543194No Hit
GTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGC550.1493712826919421No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG550.1493712826919421No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.14665544118845225No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG520.14122375818147254No Hit
GGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAG520.14122375818147254No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT510.13850791667798268No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG490.13307623367100296No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG480.1303603921675131No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG470.12764455066402325No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC440.11949702615355368No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG440.11949702615355368No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG440.11949702615355368No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC430.11678118465006382No Hit
GGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.11406534314657396No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA410.1113495016430841No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC400.10863366013959426No Hit
ACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.10863366013959426No Hit
CCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTT400.10863366013959426No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG380.10320197713261454No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA380.10320197713261454No Hit
TGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCT370.10048613562912469No Hit
GTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.10048613562912469No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGA301.2922013E-463.258165
GGCCTAC352.7687382E-454.2212756
TGGGGAA405.351311E-447.4436156
TGGCCTA405.351311E-447.4436155
GCAGAGA756.796794E-637.954899
CATGGGG756.796794E-637.954894
GGGAAAA550.002562328234.5044488
GGGGAAA550.002562328234.5044487
GAGAAAA504.9310176E-528.4661712-13
AGAAGAG350.0083927.11063854-55
GGAAAAA700.00833644727.1106389
AGAGAAA559.4349045E-525.87833610-11
AGAGTAA800.001186107923.7218075
CAGAAAA500.001650405723.72180710-11
AGTACTT5750.022.69042412-13
GGGCTTG1753.916257E-621.6885097
GGCTTGA1856.3381303E-620.5161558
AGAAAAA1058.48142E-720.3329812-13
TACTTTT6450.020.22789614-15
CATGGGC2351.07263986E-720.1887724
Produced by FastQC (version 0.11.5)