FastQCFastQC Report
Thu 26 May 2016
SRR936336_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936336_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913526
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73420.8036990736990518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47970.5251082071008378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40950.44826310362266647No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT28820.31548089490611103No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA28660.3137294395561812No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT28440.3113211884500277No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT24590.26917679409234113No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT24490.268082134498635No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA23330.2553840832116437No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG22740.24892559160877742No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG21680.23732219991549228No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20790.22757972953150757No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG20280.22199696560360624No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT18670.20437294614493734No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA18390.2013078992825601No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA18050.19758605666395918No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17520.19178436081731665No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA17300.18937610971116312No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC17130.18751518840186268No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC16920.18521640325507976No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC16830.18423120962074424No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16220.17755378609913677No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT16110.17634966054606No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG16080.17602126266794815No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG16080.17602126266794815No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.17240888600871787No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG15490.16956277106508189No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT14910.16321374542158626No Hit
GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT14880.16288534754347442No Hit
CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA14710.16102442623417396No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG14430.15795937937179674No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA13590.14876423878466513No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG13380.14646545363788224No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.14580865788165853No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT13220.1447139982879524No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT13130.14372880465361687No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA12460.1363945853757857No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC12450.13628511941641508No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT12120.1326727427571848No Hit
GGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATAC12060.13201594700096111No Hit
TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA12050.1319064810415905No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC12010.13146861720410805No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG11910.1303739576104019No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA11840.12960769589480758No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG11770.1288414341792133No Hit
ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT11290.12358706812942379No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC10950.11986522551082289No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC10880.1190989637952286No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA10700.11712857652655753No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10620.11625284885159262No Hit
GTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAAT10550.11548658713599833No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA10470.11461085946103342No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC10060.11012275512683821No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA10020.10968489128935575No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC10020.10968489128935575No Hit
GCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGCCATTGGTTGAATAAA9990.10935649341124391No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA9930.10869969765502023No Hit
CTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTCC9660.10574411675201363No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA9580.10486838907704871No Hit
GGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAA9500.1039926614020838No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9500.1039926614020838No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC9500.1039926614020838No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC9200.10070868262096536No Hit
AAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGAC9170.10038028474285351No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG200.002103789770.993751
TATAACG407.717108E-659.1485062
CCTTTAC250.005098579556.779463
GGCATTC1900.049.8038187
CTTTAGG1700.047.318812
CATTCCG2000.047.3188069
CCTTAGA857.4524E-944.5451051
CTTAGAC857.4669515E-944.5353472
ATGTATT1400.043.9485171
GACTGTG652.65977E-643.6741187
TAACGCA555.078975E-543.0123864
GGATATG901.23473E-842.0703741
CCTTTAG1800.042.0703741
CATTGGG802.3308166E-741.4039542
TTAGACG802.3318171E-741.4016883
GGACAAT6250.037.0938846
CGCTTCA905.869333E-736.8035168
TAGACGC905.8743717E-736.7994884
GACGCTT905.8743717E-736.7994886
TAGGCAT2450.036.69225
Produced by FastQC (version 0.11.5)