##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936336_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 913526 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06368948448101 33.0 31.0 34.0 30.0 34.0 2 32.170817250959466 34.0 31.0 34.0 30.0 34.0 3 32.22033636699995 34.0 31.0 34.0 30.0 34.0 4 35.73393532313256 37.0 35.0 37.0 35.0 37.0 5 35.7644018889446 37.0 35.0 37.0 35.0 37.0 6 35.872969132788775 37.0 36.0 37.0 35.0 37.0 7 35.82443849436141 37.0 36.0 37.0 35.0 37.0 8 35.83019421450511 37.0 36.0 37.0 35.0 37.0 9 37.55023611807436 39.0 38.0 39.0 35.0 39.0 10-11 37.483624987137745 39.0 37.5 39.0 35.0 39.0 12-13 37.44733756893619 39.0 37.5 39.0 35.0 39.0 14-15 38.71504642451337 40.0 38.0 41.0 34.5 41.0 16-17 38.70558418698538 40.0 38.0 41.0 35.0 41.0 18-19 38.77397195044257 40.0 38.5 41.0 35.0 41.0 20-21 38.72574234340347 40.0 38.5 41.0 35.0 41.0 22-23 38.5673609727583 40.0 38.0 41.0 34.0 41.0 24-25 38.7317542138921 40.0 39.0 41.0 35.0 41.0 26-27 38.6642383468013 40.0 38.0 41.0 35.0 41.0 28-29 38.563646792756856 40.0 38.0 41.0 35.0 41.0 30-31 38.40568358207648 40.0 38.0 41.0 34.0 41.0 32-33 38.19977865983015 40.0 38.0 41.0 34.0 41.0 34-35 37.96934351074846 40.0 38.0 41.0 33.5 41.0 36-37 37.79204915897303 40.0 38.0 41.0 33.0 41.0 38-39 37.66563896375145 40.0 37.0 41.0 33.0 41.0 40-41 37.56957984775474 40.0 37.0 41.0 33.0 41.0 42-43 37.41918785015424 40.0 37.0 41.0 33.0 41.0 44-45 37.26126349988944 40.0 37.0 41.0 33.0 41.0 46-47 37.00152431348424 40.0 37.0 41.0 31.5 41.0 48-49 36.85500139021768 40.0 37.0 41.0 31.0 41.0 50-51 36.551451190223375 39.5 37.0 40.5 31.0 41.0 52-53 36.643300792752484 40.0 37.0 40.5 31.0 41.0 54-55 36.81882726928407 40.0 37.0 41.0 31.0 41.0 56-57 36.75284556761384 40.0 37.0 41.0 31.0 41.0 58-59 36.62614911890849 40.0 37.0 41.0 31.0 41.0 60-61 36.4568840952529 40.0 36.5 41.0 31.0 41.0 62-63 36.20987908390128 39.0 36.0 41.0 30.5 41.0 64-65 35.961658453070854 39.0 36.0 41.0 30.0 41.0 66-67 35.664816327066774 39.0 35.0 40.5 29.0 41.0 68-69 35.29884918436914 38.0 35.0 40.0 29.0 41.0 70-71 34.923356861216874 37.5 35.0 40.0 28.5 41.0 72-73 34.516431387831325 37.0 35.0 39.0 28.0 41.0 74-75 34.06122431107599 36.5 34.0 39.0 27.5 40.5 76-77 33.64181315036463 36.0 34.0 38.5 26.5 40.0 78-79 33.2150245313215 35.5 34.0 37.0 26.0 39.0 80-81 32.7722965739344 35.0 34.0 37.0 26.0 39.0 82-83 32.37467078112719 35.0 33.5 36.5 25.5 39.0 84-85 31.959147303962887 35.0 33.0 36.0 25.0 37.0 86-87 31.66764492745691 35.0 33.0 36.0 24.0 37.0 88-89 31.315450791767283 35.0 33.0 35.0 22.0 36.5 90-91 30.977138034385447 35.0 32.0 35.0 20.0 36.0 92-93 30.700994826638762 35.0 32.0 35.0 18.0 36.0 94-95 30.389952776385126 35.0 32.0 35.0 9.0 36.0 96-97 30.048239458975445 34.0 31.5 35.0 2.0 35.0 98-99 29.715654507917673 34.0 31.0 35.0 2.0 35.0 100-101 26.617308100699926 31.5 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19967430115371343 1101 2 0.023211585142462354 1101 3 0.08885457127125562 1101 4 0.012626781922733699 1101 5 0.26540290458299154 1101 6 0.12155366742582174 1101 7 0.20176629864621987 1101 8 0.18102166065185088 1101 9 0.22702604712366536 1101 10-11 0.2869571608717223 1101 12-13 0.26510050884556335 1101 14-15 0.2928875483932032 1101 16-17 0.23371703936534516 1101 18-19 0.32854782796444226 1101 20-21 0.37625411392503594 1101 22-23 0.21690531966204674 1101 24-25 0.28886769725787786 1101 26-27 0.3043955951058024 1101 28-29 0.3515915828846943 1101 30-31 0.29158177508412564 1101 32-33 0.25321381733682813 1101 34-35 0.15193979407279556 1101 36-37 0.1496845466325354 1101 38-39 0.18444737296142222 1101 40-41 0.18383005032313093 1101 42-43 0.2205735609559909 1101 44-45 0.2673036726449922 1101 46-47 0.21957827060289503 1101 48-49 0.24485761529461314 1101 50-51 0.18581530144400915 1101 52-53 0.18667286382696346 1101 54-55 0.35690948891907937 1101 56-57 0.5085681683680647 1101 58-59 0.518858540068436 1101 60-61 0.39659082808767465 1101 62-63 0.37912213318656995 1101 64-65 0.3509839015482612 1101 66-67 0.4591846509123201 1101 68-69 0.5973980359298849 1101 70-71 0.5046421924150053 1101 72-73 0.4073345586838002 1101 74-75 0.4622578078515289 1101 76-77 0.3482403723019729 1101 78-79 0.36300438705921323 1101 80-81 0.5437253199895977 1101 82-83 0.7788187798324202 1101 84-85 0.4713751854848809 1101 86-87 0.307767634672544 1101 88-89 0.3268733921029643 1101 90-91 0.2690415255317724 1101 92-93 0.34521229530233555 1101 94-95 0.5740323811305039 1101 96-97 0.7207767000856862 1101 98-99 0.7574309079180281 1101 100-101 0.6903051646897289 1102 1 0.22751652409789358 1102 2 0.2205265468257238 1102 3 0.26596581403312314 1102 4 0.14133280367058632 1102 5 0.1990509256810924 1102 6 0.15574825148376448 1102 7 0.20059481929521894 1102 8 0.1574028787610473 1102 9 0.2566971538181093 1102 10-11 0.3054638591345693 1102 12-13 0.30085451729244994 1102 14-15 0.23866984051772988 1102 16-17 0.4090939958123059 1102 18-19 0.3531173618570804 1102 20-21 0.4059156653229721 1102 22-23 0.46846283299169045 1102 24-25 0.4439998470084845 1102 26-27 0.4250675235557466 1102 28-29 0.33494052320231305 1102 30-31 0.33610372360018204 1102 32-33 0.2571888206811863 1102 34-35 0.35985444616445506 1102 36-37 0.3172557813679475 1102 38-39 0.3350713338229312 1102 40-41 0.3638203039207042 1102 42-43 0.3642775760915882 1102 44-45 0.4563905685113667 1102 46-47 0.3487305246973378 1102 48-49 0.34022329649170047 1102 50-51 0.24482116839605794 1102 52-53 0.2904930713680116 1102 54-55 0.29332950153206383 1102 56-57 0.18771468928460422 1102 58-59 0.13212606925986847 1102 60-61 0.22424767827973824 1102 62-63 0.36727069893657216 1102 64-65 0.19683976945584192 1102 66-67 0.32122050238479716 1102 68-69 0.2022626373784675 1102 70-71 0.3655801403195227 1102 72-73 0.26728448893013734 1102 74-75 0.35483620905931446 1102 76-77 0.3416309737123342 1102 78-79 0.24407247810207622 1102 80-81 0.2818557306762344 1102 82-83 0.45273186485545125 1102 84-85 0.3590813410068776 1102 86-87 0.19283461730101692 1102 88-89 0.17276365907794755 1102 90-91 0.13869677043402007 1102 92-93 0.09381722201321097 1102 94-95 0.2993596500356581 1102 96-97 0.4224116113169849 1102 98-99 0.43741447280804735 1102 100-101 0.4828978988422712 1103 1 -0.09993652167801059 1103 2 -0.055976702477998685 1103 3 0.04341350381388054 1103 4 0.021633453531329394 1103 5 -0.13265120957564136 1103 6 -0.17118169209975065 1103 7 -0.04224778586081612 1103 8 -0.019775225930807494 1103 9 -0.08874489431443777 1103 10-11 -0.003053958475945251 1103 12-13 -0.05955664460142884 1103 14-15 -0.014516083927034629 1103 16-17 0.10219368873525525 1103 18-19 0.01652966636325459 1103 20-21 -0.002813714095715625 1103 22-23 -0.12951795683905942 1103 24-25 0.038849165011768605 1103 26-27 -0.027804182507246367 1103 28-29 -0.12548402867942343 1103 30-31 0.02012143409078959 1103 32-33 0.14721863572081872 1103 34-35 -0.0681306284624199 1103 36-37 -0.013473348104305671 1103 38-39 -0.03246330161233146 1103 40-41 0.10033301548251217 1103 42-43 -0.03646869256512986 1103 44-45 -0.02466259866709919 1103 46-47 0.034746914042479204 1103 48-49 0.06692025428646531 1103 50-51 0.11253880033207508 1103 52-53 0.31461022483512124 1103 54-55 0.4225321723883084 1103 56-57 0.3039165745948935 1103 58-59 0.33454608639905814 1103 60-61 0.2409940897629852 1103 62-63 0.24061212577367286 1103 64-65 0.10484787486182512 1103 66-67 0.21978509568622684 1103 68-69 0.20335615305367583 1103 70-71 0.22657510568409123 1103 72-73 0.26555360983279996 1103 74-75 0.3723949464727241 1103 76-77 0.30440234413295997 1103 78-79 0.3313698429524621 1103 80-81 0.14672754385912867 1103 82-83 0.06744924684502251 1103 84-85 0.11425880297931457 1103 86-87 0.21949854645905376 1103 88-89 0.23057984132460874 1103 90-91 0.4207372260506723 1103 92-93 0.39005106476119167 1103 94-95 0.1628389044811307 1103 96-97 0.40015031016723057 1103 98-99 0.40574447352218 1103 100-101 0.5378218437112245 1104 1 0.11042639541864929 1104 2 -0.013914438976108556 1104 3 0.11462093415443775 1104 4 0.005998778608727662 1104 5 0.01720259159394999 1104 6 0.043610189565193025 1104 7 0.04386332525029246 1104 8 -0.012267485992722982 1104 9 0.07005283530372708 1104 10-11 0.16859206835584217 1104 12-13 0.20815491370609607 1104 14-15 0.15107129137395958 1104 16-17 0.2049542666506312 1104 18-19 -0.02040273817750915 1104 20-21 0.11161267049320855 1104 22-23 0.2527747848190103 1104 24-25 0.309893196659516 1104 26-27 0.2525406747339005 1104 28-29 0.3299769276329272 1104 30-31 0.29989439590709566 1104 32-33 0.3157471874924056 1104 34-35 0.28432035192974325 1104 36-37 0.4594884682011653 1104 38-39 0.3728299560416062 1104 40-41 0.05572087237965917 1104 42-43 0.13771431397081102 1104 44-45 0.25176928987779235 1104 46-47 0.1915795593813172 1104 48-49 0.287856786794201 1104 50-51 0.20560725578442884 1104 52-53 0.09203970539928008 1104 54-55 0.09156382013512854 1104 56-57 0.1402168841924194 1104 58-59 0.15835499592778035 1104 60-61 0.23648772581390176 1104 62-63 0.08743015122946929 1104 64-65 0.1691280606203449 1104 66-67 0.18129180363256836 1104 68-69 0.1380267756886937 1104 70-71 0.10397018652344059 1104 72-73 0.148538645952506 1104 74-75 0.038760615547374755 1104 76-77 0.05497423955779368 1104 78-79 0.12052532970857044 1104 80-81 0.02681010319864896 1104 82-83 0.1567208326723346 1104 84-85 -0.1156173580113915 1104 86-87 0.0731363173568873 1104 88-89 0.14138135491250026 1104 90-91 0.12854166361517017 1104 92-93 0.014212571469141011 1104 94-95 -0.010226110998740268 1104 96-97 0.15124250142969586 1104 98-99 0.2367644253625656 1104 100-101 0.6358266596726985 1105 1 0.10615980650303669 1105 2 0.16492755631023215 1105 3 0.25487897045446317 1105 4 0.05604615815534686 1105 5 0.17019722191199804 1105 6 0.03745327822569067 1105 7 0.16868777795578893 1105 8 0.19458432536379178 1105 9 0.13469311520751148 1105 10-11 0.2555663376174522 1105 12-13 0.1979573774425205 1105 14-15 0.2346026469149436 1105 16-17 0.19048528203075676 1105 18-19 0.3238643366821776 1105 20-21 0.3642744291247837 1105 22-23 0.3575540447261929 1105 24-25 0.38578551145408824 1105 26-27 0.2996553818311085 1105 28-29 0.4628923490679142 1105 30-31 0.4064254841788397 1105 32-33 0.45947243471644583 1105 34-35 0.24745177550288844 1105 36-37 0.15773906955694628 1105 38-39 0.24580660642914864 1105 40-41 0.25075938344703985 1105 42-43 0.27871098525394444 1105 44-45 0.3695402644386192 1105 46-47 0.31242170045087647 1105 48-49 0.42770663007723186 1105 50-51 0.4176878253108782 1105 52-53 0.23901591843715408 1105 54-55 0.41646845361182017 1105 56-57 0.35427038663429045 1105 58-59 0.3683873112082878 1105 60-61 0.47382523826622247 1105 62-63 0.14986667478561344 1105 64-65 0.2550818473394898 1105 66-67 0.42671785509404714 1105 68-69 0.3694236219117002 1105 70-71 0.3651724121472739 1105 72-73 0.281718906904473 1105 74-75 0.17516511538944002 1105 76-77 0.23880445070049205 1105 78-79 0.19756204278289857 1105 80-81 0.3843653185933604 1105 82-83 0.3271171200596541 1105 84-85 0.04605623335823594 1105 86-87 0.14057563509141602 1105 88-89 0.16499580688907756 1105 90-91 0.295857065612406 1105 92-93 0.17684833209309048 1105 94-95 0.23606006146280833 1105 96-97 0.4170077418505258 1105 98-99 0.49008706792640666 1105 100-101 0.5200431963493273 1106 1 0.07126940576194585 1106 2 0.21037485602992234 1106 3 -0.017917615245906404 1106 4 0.06326605445110545 1106 5 -0.016184679171963978 1106 6 0.014306299724466953 1106 7 -0.03108273731712785 1106 8 0.09492840207123976 1106 9 0.10269608677907627 1106 10-11 0.012793001156147454 1106 12-13 -0.017892107043948613 1106 14-15 0.026177579844038235 1106 16-17 0.030387352506330956 1106 18-19 -0.05136123808232185 1106 20-21 0.022991089174588808 1106 22-23 0.09488802169951782 1106 24-25 -0.02442370162083307 1106 26-27 -0.02751675276784482 1106 28-29 -0.15391402356956263 1106 30-31 -0.0480377381115602 1106 32-33 -0.08405371992557775 1106 34-35 -0.13779082263717157 1106 36-37 -0.05177176404707495 1106 38-39 -0.09577449221018952 1106 40-41 -0.1599122578951082 1106 42-43 -0.047755100332125267 1106 44-45 -0.024151612463718664 1106 46-47 0.008418349913711154 1106 48-49 -0.09847959241768223 1106 50-51 0.07045908648434818 1106 52-53 0.08959745018004384 1106 54-55 0.10864612231166149 1106 56-57 0.23310666146254988 1106 58-59 -0.038831532405239955 1106 60-61 0.1675398230281928 1106 62-63 0.21044477779207682 1106 64-65 0.13813862601154625 1106 66-67 0.1338308289514316 1106 68-69 0.05092258126011728 1106 70-71 0.043265192551743326 1106 72-73 0.05340491284761839 1106 74-75 -0.04441011229069147 1106 76-77 0.012533412094121843 1106 78-79 0.029562229258033312 1106 80-81 -0.0025826247663118806 1106 82-83 0.01022517939484402 1106 84-85 0.01784209373046508 1106 86-87 0.06042215402165141 1106 88-89 -0.09579343409695795 1106 90-91 0.048918115166667775 1106 92-93 -0.1930927778550533 1106 94-95 -0.08528322122147003 1106 96-97 0.02781382575230751 1106 98-99 0.16757380413025302 1106 100-101 0.17369946251551838 1107 1 -0.09394984783227045 1107 2 0.04041917852886456 1107 3 0.11538819758155228 1107 4 -0.039014009115057036 1107 5 0.14510824660958122 1107 6 0.011413542790542408 1107 7 -0.027094333175398333 1107 8 0.047067552370450016 1107 9 -0.008293285753396162 1107 10-11 0.07643520561074268 1107 12-13 0.021411520696723585 1107 14-15 0.04132420413326798 1107 16-17 -0.027995434969156463 1107 18-19 0.12942675883869015 1107 20-21 0.06503693875123417 1107 22-23 0.02815131224156886 1107 24-25 0.22009495852368843 1107 26-27 0.15434516464580383 1107 28-29 0.16822643033255247 1107 30-31 0.11106234134614112 1107 32-33 -0.0869666516664438 1107 34-35 -0.10451170263121412 1107 36-37 -0.10862747382782345 1107 38-39 -0.09216009795100888 1107 40-41 0.18861373967150286 1107 42-43 -0.058719331951323284 1107 44-45 0.13552888067063407 1107 46-47 0.21978359461859753 1107 48-49 0.18082353871236023 1107 50-51 0.19919918530986536 1107 52-53 0.21784249028134894 1107 54-55 0.33693182240850916 1107 56-57 0.2582475747295021 1107 58-59 0.37002024173624193 1107 60-61 0.29487078918418064 1107 62-63 0.2939234731959459 1107 64-65 0.08805389182239054 1107 66-67 0.14783059306137858 1107 68-69 0.04210463871796577 1107 70-71 0.031961093030972165 1107 72-73 -0.1127998024703416 1107 74-75 -0.08414361861005659 1107 76-77 0.10571308582093764 1107 78-79 -0.017738381573046524 1107 80-81 0.011763214878108386 1107 82-83 0.2574596789354757 1107 84-85 0.14780975196303103 1107 86-87 -0.14284833737457348 1107 88-89 0.062111676026450624 1107 90-91 0.23642742661602512 1107 92-93 0.03650583660128248 1107 94-95 0.0503876997429451 1107 96-97 0.27697424340184895 1107 98-99 0.3397766447430719 1107 100-101 0.2664461538471805 1108 1 -0.31209806716021404 1108 2 -0.10715558900066213 1108 3 -0.2664739315375009 1108 4 -0.1519746997999718 1108 5 -0.08941269421208631 1108 6 -0.14501801701608485 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-0.7471677928722116 2316 70-71 -0.41020775298961354 2316 72-73 -0.46663171349901944 2316 74-75 -0.5597187657640106 2316 76-77 -0.7306896683964013 2316 78-79 -0.7248649590093734 2316 80-81 -0.4648538764104231 2316 82-83 -0.6666371192267775 2316 84-85 -0.8888220196724603 2316 86-87 -0.9485505406282826 2316 88-89 -1.0796703338392213 2316 90-91 -1.1392167161658726 2316 92-93 -0.8970285420475257 2316 94-95 -0.904910343344774 2316 96-97 -1.3064719129708813 2316 98-99 -1.0913508980312123 2316 100-101 -1.53878039014279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3731.0 3 768.0 4 612.0 5 486.0 6 548.0 7 601.0 8 803.0 9 954.0 10 1177.0 11 1543.0 12 2328.0 13 4051.0 14 4428.0 15 3668.0 16 4893.0 17 6981.0 18 6241.0 19 3188.0 20 2783.0 21 2713.0 22 3079.0 23 3426.0 24 3898.0 25 4393.0 26 5209.0 27 6362.0 28 7879.0 29 9433.0 30 11924.0 31 14972.0 32 19219.0 33 25303.0 34 35585.0 35 60042.0 36 86237.0 37 182673.0 38 309038.0 39 72332.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.30175222628788 23.651495454331393 14.681288448711868 21.365463870668854 2 13.537208739644472 23.818892249953745 42.23128724404725 20.412611766354534 3 15.352462237948275 31.976413839462154 31.272573205885156 21.398550716704417 4 9.648108737728032 19.737723654134154 44.16835255686048 26.44581505127734 5 9.099906069158498 42.689706362117626 37.688710125919876 10.521677442804 6 24.309891842185927 44.15323816613391 19.933331873713712 11.603538117966458 7 25.098470235606047 35.09448544786168 25.662689906882957 14.144354409649317 8 23.48377943135776 37.70038283891973 22.880455071438817 15.935382658283695 9 26.88140610869884 16.761588200388445 24.200830755014856 32.15617493589786 10-11 21.51206450751281 29.911024120257558 31.11742013736089 17.459491234868736 12-13 22.168341653529513 29.075363461201608 30.435168155543877 18.321126729724995 14-15 20.9358527333337 30.519790463574708 27.76828380471731 20.776072998374286 16-17 17.42424325368889 33.54537302745604 29.75274565856938 19.277638060285696 18-19 17.190508982265378 32.85612938753748 32.892586141397466 17.060775488799678 20-21 18.192262578195514 32.185927571619125 32.76945535035477 16.852354499830597 22-23 18.668059942995367 31.199117178079 32.37714362660925 17.755679252316394 24-25 18.097181307404302 31.8696701625455 32.742097866723746 17.29105066332645 26-27 17.20398795337681 32.486800058240675 32.75578000352509 17.553431984857426 28-29 17.870302802702795 30.3742101197733 33.89421695176168 17.861270125762235 30-31 17.818887891974093 31.929927899156258 33.05290770971568 17.198276499153963 32-33 17.033282242172103 33.04543463980731 32.748303043573465 17.172980074447118 34-35 17.432580921007133 31.691440326417347 33.31814823799838 17.55783051457714 36-37 18.040602866039364 31.011430384989623 32.17157513356351 18.776391615407505 38-39 17.321592987913874 32.5532226181139 32.90099982509386 17.224184568878368 40-41 16.844121064352297 31.94679778890757 34.118610761471366 17.090470385268773 42-43 17.28827451152966 31.662669424397112 32.34344745643373 18.7056086076395 44-45 17.138427580986082 32.09349613623685 32.69399413971165 18.074082143065418 46-47 17.427349820716355 31.210650169379996 33.13971689854972 18.222283111353935 48-49 17.274175922736532 32.20655608171386 32.51441921990397 18.004848775645634 50-51 17.96848946438693 31.266639885096055 31.762080597852705 19.00279005266431 52-53 17.37427794486795 31.930546247294185 32.50054198842071 18.19463381941716 54-55 18.42165057522613 31.56698849547736 31.98929596898824 18.022064960308267 56-57 18.183323559216298 32.18922983992087 31.110519407215385 18.516927193647447 58-59 17.66082489029134 31.209686159242455 32.56458878988474 18.56490016058146 60-61 18.157403403846136 33.578461304877365 30.500774001472088 17.763361289804415 62-63 17.785333658256974 33.056891525929075 32.304744636972096 16.85303017884186 64-65 17.80350567158661 31.28844076896118 32.35310461391012 18.554948945542098 66-67 18.130880497402213 32.49351240810391 31.137580950516092 18.238026143977788 68-69 17.621934546062757 31.914062478508193 32.027896848124925 18.43610612730413 70-71 18.42793011091314 30.758635333231755 33.08961009599491 17.7238244598602 72-73 18.178937483392847 31.676459957716744 31.483308128055647 18.661294430834758 74-75 17.820559729516336 32.22073359615072 31.190683829577686 18.768022844755254 76-77 17.666009081496917 31.35625015816152 32.926202660194235 18.051538100147326 78-79 16.91625792453619 31.44888357835856 32.67521999458678 18.959638502518466 80-81 17.874859370143284 32.516593635415894 30.78078796731061 18.827759027130217 82-83 19.05334317726448 31.002441544323545 31.4072383702059 18.536976908206075 84-85 16.922707052168644 31.427059298601183 31.87119639188813 19.779037257342043 86-87 17.850238383153744 31.78962865483242 31.83358622230096 18.526546739712884 88-89 18.378116942499364 31.048923575689745 31.956425806891804 18.61653367491909 90-91 17.47047233260317 30.56903364998294 33.02332493092782 18.93716908648607 92-93 18.570094471125138 31.91090096738388 31.530859265153655 17.988145296337326 94-95 17.886039312382646 29.998013970425212 32.50420724686238 19.611739470329763 96-97 18.68307379578206 30.27564108910074 31.755483617620172 19.285801497497022 98-99 17.74397470268784 30.904186430191793 31.979157214545957 19.372681652574414 100-101 17.84786790902521 30.509629525337935 32.07959029006821 19.562912275568646 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1258.0 1 839.0 2 902.5 3 1338.0 4 943.0 5 1166.0 6 2845.0 7 4661.0 8 5464.5 9 4900.5 10 3515.0 11 2673.5 12 2592.0 13 2554.5 14 2302.0 15 2035.0 16 1834.0 17 1600.0 18 1470.0 19 1376.0 20 1417.0 21 1640.5 22 1915.5 23 2399.0 24 2598.5 25 3091.0 26 5280.5 27 7573.5 28 7754.0 29 9545.5 30 15779.5 31 21758.5 32 30513.0 33 41438.5 34 49316.5 35 56708.5 36 67515.5 37 73837.5 38 67252.0 39 60962.5 40 54685.5 41 45933.5 42 36320.5 43 34311.5 44 35885.5 45 31685.5 46 24344.5 47 16965.5 48 15667.0 49 13204.5 50 10523.0 51 6967.0 52 3377.0 53 2380.5 54 1537.5 55 1205.5 56 972.5 57 704.5 58 582.0 59 444.0 60 316.0 61 301.0 62 282.5 63 228.0 64 157.5 65 89.0 66 59.5 67 69.5 68 70.0 69 67.5 70 58.5 71 33.0 72 16.5 73 15.5 74 13.0 75 7.5 76 7.0 77 7.5 78 8.5 79 6.5 80 5.5 81 5.5 82 2.5 83 1.0 84 2.0 85 2.5 86 5.0 87 6.0 88 4.5 89 3.0 90 1.5 91 3.0 92 3.5 93 1.5 94 3.0 95 7.5 96 9.5 97 5.5 98 1.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050244875351112064 2 0.013683244921326816 3 0.011931789571396983 4 0.006786889480978101 5 0.009633004424614077 6 0.005035434131048268 7 0.005692229887271955 8 0.009523538465243463 9 0.015325234311886033 10-11 0.0021893191874122904 12-13 0.00941407250587285 14-15 0.008866742709019776 16-17 0.01915654288985754 18-19 0.01258858532762067 20-21 0.0033387117608037428 22-23 0.010016135282411229 24-25 0.023261516366255584 26-27 0.00820994695279609 28-29 0.019211275869542847 30-31 0.016693558804018717 32-33 0.01379271088069743 34-35 0.2785908665982139 36-37 0.4927610161068212 38-39 0.48898444050853507 40-41 0.50945457491084 42-43 0.4998215704862259 44-45 0.5141616111637765 46-47 0.5222073591775166 48-49 0.5301436412318861 50-51 0.5408713052502063 52-53 0.5292679135569212 54-55 0.5155299356559091 56-57 0.5104397685451756 58-59 0.5080862504187073 60-61 0.5049664705766448 62-63 0.5029413503282885 64-65 0.5190328463557687 66-67 0.4907358958584649 68-69 0.5199085740307337 70-71 0.5004236332627643 72-73 0.509618773849896 74-75 0.5236304166493346 76-77 0.5084693812765045 78-79 0.5079220514796513 80-81 0.5110418313217139 82-83 0.511753360057623 84-85 0.5128480196513291 86-87 0.5139426792450352 88-89 0.5279543220444738 90-91 0.553459890577827 92-93 0.5409260382298916 94-95 0.5120270249560495 96-97 0.48263541486503936 98-99 0.4749180647294111 100-101 0.48110289143385077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 913526.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.9899791875602 #Duplication Level Percentage of deduplicated Percentage of total 1 70.37168074086507 8.437549874766031 2 9.773147732191847 2.343596758118628 3 4.1009746475390605 1.475118020181161 4 2.3348895592857444 1.1198110888435076 5 1.6013339452757267 0.9599980338094816 6 1.189560715065239 0.8557684929582867 7 0.9138502701937982 0.7669932004118933 8 0.7376531109184992 0.7075556358041474 9 0.6043642809249844 0.6521683634995815 >10 5.630082324955018 14.837541680324978 >50 1.1722110758508626 10.0502668875917 >100 1.360276092692979 34.10113700618253 >500 0.15976397061830291 12.93795629642629 >1k 0.04929859664793348 9.950876342001246 >5k 9.129369749617311E-4 0.8036623190805336 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7342 0.8036990736990518 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4797 0.5251082071008378 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4095 0.44826310362266647 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2882 0.31548089490611103 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 2866 0.3137294395561812 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2844 0.3113211884500277 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2459 0.26917679409234113 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 2449 0.268082134498635 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 2333 0.2553840832116437 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 2274 0.24892559160877742 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 2168 0.23732219991549228 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2079 0.22757972953150757 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2028 0.22199696560360624 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1867 0.20437294614493734 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1839 0.2013078992825601 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1805 0.19758605666395918 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1752 0.19178436081731665 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1730 0.18937610971116312 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1713 0.18751518840186268 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 1692 0.18521640325507976 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1683 0.18423120962074424 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1622 0.17755378609913677 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1611 0.17634966054606 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1608 0.17602126266794815 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1608 0.17602126266794815 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1575 0.17240888600871787 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1549 0.16956277106508189 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 1491 0.16321374542158626 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 1488 0.16288534754347442 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 1471 0.16102442623417396 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1443 0.15795937937179674 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 1359 0.14876423878466513 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 1338 0.14646545363788224 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1332 0.14580865788165853 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1322 0.1447139982879524 No Hit GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 1313 0.14372880465361687 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1246 0.1363945853757857 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1245 0.13628511941641508 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1212 0.1326727427571848 No Hit GGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATAC 1206 0.13201594700096111 No Hit TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA 1205 0.1319064810415905 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1201 0.13146861720410805 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1191 0.1303739576104019 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 1184 0.12960769589480758 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1177 0.1288414341792133 No Hit ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT 1129 0.12358706812942379 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1095 0.11986522551082289 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 1088 0.1190989637952286 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1070 0.11712857652655753 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1062 0.11625284885159262 No Hit GTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAAT 1055 0.11548658713599833 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1047 0.11461085946103342 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1006 0.11012275512683821 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 1002 0.10968489128935575 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1002 0.10968489128935575 No Hit GCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGCCATTGGTTGAATAAA 999 0.10935649341124391 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 993 0.10869969765502023 No Hit CTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTCC 966 0.10574411675201363 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 958 0.10486838907704871 No Hit GGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAA 950 0.1039926614020838 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 950 0.1039926614020838 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 950 0.1039926614020838 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 920 0.10070868262096536 No Hit AAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGAC 917 0.10038028474285351 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.0946595937061452E-4 0.0 16-17 0.0 0.0 0.0 8.757276749649162E-4 0.0 18-19 0.0 0.0 0.0 0.001477790451503296 0.0 20-21 0.0 0.0 0.0 0.0026271830248947485 0.0 22-23 0.0 0.0 0.0 0.0057469628669572625 0.0 24-25 0.0 5.473297968530726E-5 0.0 0.008812009729334469 0.0 26-27 0.0 1.0946595937061452E-4 0.0 0.01116552785580268 0.0 28-29 0.0 1.0946595937061452E-4 0.0 0.014230574718179886 0.0 30-31 0.0 1.0946595937061452E-4 0.0 0.02101746419915799 0.0 32-33 0.0 1.0946595937061452E-4 0.0 0.03267558887212844 0.0 34-35 0.0 1.0946595937061452E-4 0.0 0.05013540939174145 0.0 36-37 0.0 1.0946595937061452E-4 0.0 0.07892495670621308 0.0 38-39 0.0 1.0946595937061452E-4 0.0 0.12402493196690625 0.0 40-41 0.0 1.0946595937061452E-4 0.0 0.17706118928196898 0.0 42-43 0.0 1.0946595937061452E-4 0.0 0.22670400185654266 0.0 44-45 0.0 1.0946595937061452E-4 0.0 0.27771513892324906 0.0 46-47 0.0 1.0946595937061452E-4 0.0 0.34706182418453335 0.0 48-49 0.0 1.0946595937061452E-4 0.0 0.42275753508931324 0.0 50-51 0.0 1.0946595937061452E-4 0.0 0.4959902619082544 0.0 52-53 0.0 1.0946595937061452E-4 0.0 0.5724522345286286 0.0 54-55 0.0 1.0946595937061452E-4 0.0 0.6529644476457156 0.0 56-57 0.0 1.0946595937061452E-4 0.0 0.7480356333590943 0.0 58-59 0.0 1.0946595937061452E-4 0.0 0.8570089959125411 0.0 60-61 0.0 1.0946595937061452E-4 0.0 0.9721124521907423 0.0 62-63 0.0 1.0946595937061452E-4 0.0 1.0820162753988392 0.0 64-65 0.0 1.0946595937061452E-4 0.0 1.2028666945439976 0.0 66-67 0.0 1.0946595937061452E-4 0.0 1.3450629757664259 0.0 68-69 0.0 1.0946595937061452E-4 0.0 1.4967827954540978 0.0 70-71 0.0 1.0946595937061452E-4 0.0 1.6730229900407871 0.0 72-73 0.0 1.0946595937061452E-4 0.0 1.846526535643211 0.0 74-75 0.0 1.0946595937061452E-4 0.0 2.0192090865503554 0.0 76-77 0.0 1.0946595937061452E-4 0.0 2.2188749964423566 0.0 78-79 0.0 1.0946595937061452E-4 0.0 2.451599626064283 0.0 80-81 0.0 1.0946595937061452E-4 0.0 2.7164525147614844 0.0 82-83 0.0 1.0946595937061452E-4 0.0 2.9794992151290716 0.0 84-85 0.0 1.0946595937061452E-4 0.0 3.2380030781827775 0.0 86-87 0.0 1.0946595937061452E-4 0.0 3.521848310830781 0.0 88-89 0.0 1.0946595937061452E-4 0.0 3.804544150905393 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACACG 20 0.0021037897 70.99375 1 TATAACG 40 7.717108E-6 59.148506 2 CCTTTAC 25 0.0050985795 56.77946 3 GGCATTC 190 0.0 49.803818 7 CTTTAGG 170 0.0 47.31881 2 CATTCCG 200 0.0 47.318806 9 CCTTAGA 85 7.4524E-9 44.545105 1 CTTAGAC 85 7.4669515E-9 44.535347 2 ATGTATT 140 0.0 43.948517 1 GACTGTG 65 2.65977E-6 43.674118 7 TAACGCA 55 5.078975E-5 43.012386 4 GGATATG 90 1.23473E-8 42.070374 1 CCTTTAG 180 0.0 42.070374 1 CATTGGG 80 2.3308166E-7 41.403954 2 TTAGACG 80 2.3318171E-7 41.401688 3 GGACAAT 625 0.0 37.093884 6 CGCTTCA 90 5.869333E-7 36.803516 8 TAGACGC 90 5.8743717E-7 36.799488 4 GACGCTT 90 5.8743717E-7 36.799488 6 TAGGCAT 245 0.0 36.6922 5 >>END_MODULE