FastQCFastQC Report
Thu 26 May 2016
SRR936336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936336_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913526
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70540.7721728774003148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48550.5314572327443334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40750.4460737844352542No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA29950.3278505483149905No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT29260.3202973971184181No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT28730.3144957012717755No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA24430.2674253387424113No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT24110.2639224280425516No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG22870.2503486490805954No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT22710.24859719373066558No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21010.22998798063766113No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG20820.2279081274096194No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG20640.22593774014094836No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT18860.206452799372979No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA18690.20459187806367857No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC18380.20119843332318949No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA17770.194521009801582No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17630.1929884863703934No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC17530.19189382677668726No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17100.18718679052375084No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA16960.18565426709256222No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG16960.18565426709256222No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT16790.18379334578326179No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC16560.18127562871773764No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG16140.17667805842417184No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG16130.17656859246480122No Hit
CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA15470.16934383914634066No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG15350.1680302476338933No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT14860.1626664156247332No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.1545659346313077No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14020.15347127503760155No Hit
GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT13880.15193875160641296No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA13840.1515008877689305No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT13730.15029676221585375No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT13420.1469033174753647No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA13080.1431814748567638No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC13040.14274361101928132No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC13020.1425246791005401No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT12780.13989749607564536No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG12370.13540939174145017No Hit
GGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATAC11840.12960769589480758No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG11800.12916983205732513No Hit
ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT11630.1273089107480247No Hit
TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA11530.12621425115431853No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC11530.12621425115431853No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG11470.12555745539809485No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA11400.12479119368250055No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11270.12336813621068256No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA10870.11898949783585798No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC10790.11811377016089307No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA10740.11756644036403999No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC10670.11680017864844569No Hit
GTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAAT10350.11329726794858602No Hit
GGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAA10330.1130783360298448No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA10320.11296887007047418No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10190.1115458125986562No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC10050.1100132891674676No Hit
GCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGCCATTGGTTGAATAAA9920.10859023169564962No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG9880.10815236785816713No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT9710.1062914465488667No Hit
CAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTAC9640.1055251848332724No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC9530.10432105928019564No Hit
AAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGAC9510.10410212736145441No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT9510.10410212736145441No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC9360.1024601379708952No Hit
GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG9330.10213174009278336No Hit
CTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTCC9270.10147494433655965No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT9230.1010370804990772No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA9180.10048975070222413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG301.3777644E-679.063482
TCACGCA301.3791105E-679.050494
ACAGTCA200.002086055571.145458
GGTAACG200.002086055571.145458
GTAACGA200.002086055571.145459
TATCACG353.4430104E-667.768692
ATCACGC353.4463683E-667.757573
GTATGGT701.8189894E-1161.1928181
GGGCGAC407.6102006E-659.287877
GGCGACT407.6102006E-659.287878
CTCTGTA250.005046934456.92572
CTGACCA250.005050216356.9163559
TACAGTC250.005050216356.9163557
CACGCAG451.528955E-552.7003255
GTATCAC502.7935515E-547.5944141
TACAGGG708.000279E-847.4380872
TAACGCA502.8511746E-547.4302944
TCAGGCC502.8511746E-547.4302943
TGGGCGA1001.2732926E-1147.4302946
ATGGGCG1151.8189894E-1245.3681075
Produced by FastQC (version 0.11.5)