##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936336_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 913526 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52902599378671 34.0 31.0 34.0 31.0 34.0 2 32.66932960857162 34.0 31.0 34.0 31.0 34.0 3 32.75313565240617 34.0 31.0 34.0 31.0 34.0 4 36.2327290082603 37.0 35.0 37.0 35.0 37.0 5 36.219506615027925 37.0 37.0 37.0 35.0 37.0 6 36.261961892710225 37.0 37.0 37.0 35.0 37.0 7 36.21708632266624 37.0 37.0 37.0 35.0 37.0 8 36.19779951528473 37.0 37.0 37.0 35.0 37.0 9 37.97790976939901 39.0 38.0 39.0 35.0 39.0 10-11 37.916944345316935 39.0 38.0 39.0 35.0 39.0 12-13 37.897923540216695 39.0 38.0 39.0 35.0 39.0 14-15 39.223880874764376 41.0 39.0 41.0 36.0 41.0 16-17 39.1766895523499 41.0 39.0 41.0 36.0 41.0 18-19 39.29734895339597 41.0 39.0 41.0 36.0 41.0 20-21 39.27342243132653 41.0 39.0 41.0 36.0 41.0 22-23 39.137574081088005 40.0 39.0 41.0 36.0 41.0 24-25 39.28500830846632 41.0 39.0 41.0 36.0 41.0 26-27 39.183750106729306 41.0 39.0 41.0 35.5 41.0 28-29 39.10421323531021 41.0 39.0 41.0 35.0 41.0 30-31 38.97027397140312 40.0 38.5 41.0 35.0 41.0 32-33 38.77808513386592 40.0 38.0 41.0 35.0 41.0 34-35 38.6599412605662 40.0 38.0 41.0 35.0 41.0 36-37 38.53577566484151 40.0 38.0 41.0 35.0 41.0 38-39 38.427436657522605 40.0 38.0 41.0 35.0 41.0 40-41 38.35381806319688 40.0 38.0 41.0 34.5 41.0 42-43 38.346046527411374 40.0 38.0 41.0 35.0 41.0 44-45 38.20644349476643 40.0 38.0 41.0 34.0 41.0 46-47 37.99796010184713 40.0 38.0 41.0 33.5 41.0 48-49 37.80558188820022 40.0 38.0 41.0 33.0 41.0 50-51 37.681071474703515 40.0 38.0 41.0 33.0 41.0 52-53 37.57721947705922 40.0 38.0 41.0 33.0 41.0 54-55 37.48155991181422 40.0 38.0 41.0 33.0 41.0 56-57 37.321657511663595 40.0 37.5 41.0 33.0 41.0 58-59 37.160333148700744 40.0 37.0 41.0 32.0 41.0 60-61 36.99539531441907 40.0 37.0 41.0 32.0 41.0 62-63 36.75393365924998 39.0 36.0 41.0 31.5 41.0 64-65 36.4599097343699 39.0 36.0 41.0 31.0 41.0 66-67 36.15878420537565 39.0 35.5 40.0 31.0 41.0 68-69 35.80575703373522 38.0 35.0 40.0 30.5 41.0 70-71 35.46875786786583 37.5 35.0 40.0 30.0 41.0 72-73 35.05602850931446 37.0 35.0 39.0 29.5 41.0 74-75 34.57761574383214 36.5 34.5 39.0 29.0 40.5 76-77 33.777054511858445 36.0 34.0 38.0 28.0 39.0 78-79 33.76042334865127 36.0 34.0 37.0 29.0 39.0 80-81 33.52808130255734 35.0 34.0 37.0 29.0 39.0 82-83 33.1859443518849 35.0 34.0 36.5 29.0 39.0 84-85 32.796244441865916 35.0 34.0 36.0 28.5 37.0 86-87 32.412455693652944 35.0 33.5 36.0 26.5 37.0 88-89 31.86781985405998 35.0 33.0 35.0 25.5 36.5 90-91 31.63856255870112 35.0 33.0 35.0 25.0 36.0 92-93 31.38028912149189 35.0 33.0 35.0 24.5 36.0 94-95 31.255210032336244 35.0 33.0 35.0 24.0 36.0 96-97 31.059171824337785 35.0 32.5 35.0 23.5 35.5 98-99 30.75583672495364 35.0 32.0 35.0 19.5 35.0 100-101 27.689278137677526 32.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0914955763862011 1101 2 0.14283716569728 1101 3 0.2715081547897995 1101 4 0.16892866972921894 1101 5 0.09824961635186469 1101 6 0.05656180961716473 1101 7 0.14127168656450806 1101 8 0.02938216117154724 1101 9 0.23960938928239273 1101 10-11 0.0917106180513585 1101 12-13 0.20515204463608683 1101 14-15 0.3591683524518601 1101 16-17 0.2520002524591334 1101 18-19 0.23235689141489502 1101 20-21 0.22366740724577738 1101 22-23 0.18138243089796902 1101 24-25 0.23878442611517414 1101 26-27 0.3408301942559149 1101 28-29 0.31153002531914353 1101 30-31 0.29321240851372465 1101 32-33 0.29719623030081266 1101 34-35 0.36289632052287146 1101 36-37 0.25810710870064213 1101 38-39 0.22379521329005314 1101 40-41 0.27832909251551996 1101 42-43 0.4360906644266933 1101 44-45 0.3411658640966664 1101 46-47 0.6028439005992468 1101 48-49 0.3028668951124871 1101 50-51 0.19832193571732404 1101 52-53 0.1323831994704605 1101 54-55 0.17981104277340165 1101 56-57 0.06903193077273784 1101 58-59 0.1636217303380363 1101 60-61 0.3042570705351366 1101 62-63 0.3250970078717401 1101 64-65 0.4545512408219068 1101 66-67 0.3772258620792712 1101 68-69 0.24883572426183065 1101 70-71 0.4602367150326998 1101 72-73 0.5545903080284376 1101 74-75 0.5142550512984343 1101 76-77 0.25548291791802313 1101 78-79 0.23531995343645207 1101 80-81 0.23572180397549403 1101 82-83 0.2861623473647583 1101 84-85 0.41461775374683185 1101 86-87 0.47557788296485626 1101 88-89 0.5039497634054015 1101 90-91 0.601413321386115 1101 92-93 0.7598095120645922 1101 94-95 0.598247709475082 1101 96-97 0.4683202109606164 1101 98-99 0.3301303799009112 1101 100-101 0.8338422200694495 1102 1 0.18152940213580848 1102 2 0.1919838777197569 1102 3 0.16908875371578347 1102 4 0.17259981464484753 1102 5 0.06687002264306585 1102 6 0.10106273648091957 1102 7 0.16376867664610728 1102 8 0.2114621581138536 1102 9 0.09932617029410551 1102 10-11 0.1573784377237999 1102 12-13 0.14629332925014893 1102 14-15 0.17056782117737868 1102 16-17 0.16721515107743556 1102 18-19 0.33439121403992544 1102 20-21 0.33961615263534384 1102 22-23 0.2503401391556537 1102 24-25 0.2261829010898495 1102 26-27 0.21994613822365494 1102 28-29 0.2100938642550645 1102 30-31 0.19027319931006303 1102 32-33 0.2266934305989423 1102 34-35 0.3341931653639634 1102 36-37 0.3267991797156071 1102 38-39 0.2844914121931126 1102 40-41 0.10044387073708805 1102 42-43 0.2259859383770504 1102 44-45 0.22195589601135168 1102 46-47 0.24534108178677627 1102 48-49 0.14981015576186252 1102 50-51 0.1643366126527468 1102 52-53 0.22624201040936498 1102 54-55 0.03137142269198989 1102 56-57 -0.059401765024837516 1102 58-59 -0.01042608436565473 1102 60-61 -0.027304073807172813 1102 62-63 -0.005010775872790418 1102 64-65 0.07479795857755533 1102 66-67 0.05033248007762836 1102 68-69 0.10197272810305691 1102 70-71 0.16062274901133833 1102 72-73 -0.05368266768199703 1102 74-75 -0.13781367039400294 1102 76-77 -0.1117729920328685 1102 78-79 -0.07506665385997024 1102 80-81 0.07704846134166843 1102 82-83 0.0946656057208557 1102 84-85 -0.04643199112674523 1102 86-87 -0.15476209448463862 1102 88-89 -0.1337755027096108 1102 90-91 0.08721128419690061 1102 92-93 0.06322744162294214 1102 94-95 -0.031653679865026874 1102 96-97 -0.07910559216205115 1102 98-99 0.028384627230206405 1102 100-101 0.5566258690460764 1103 1 -0.0541308135322609 1103 2 0.05180677281366286 1103 3 0.10342068258816539 1103 4 0.07561881057429076 1103 5 0.07732671049566875 1103 6 0.11571673919462455 1103 7 0.15272461465939102 1103 8 0.03911159037836853 1103 9 0.04772651307779796 1103 10-11 0.07849712657619534 1103 12-13 0.05951638859458086 1103 14-15 0.0013014139789149226 1103 16-17 0.3085143369661765 1103 18-19 0.11556667644083518 1103 20-21 0.05730343393220494 1103 22-23 0.147931726672617 1103 24-25 0.024560927227106788 1103 26-27 0.23836540552352403 1103 28-29 0.24820534036732766 1103 30-31 0.19387956937362816 1103 32-33 0.09929037411104957 1103 34-35 0.029918188573283544 1103 36-37 0.24645032589857152 1103 38-39 0.1695142644390515 1103 40-41 0.07632760993583076 1103 42-43 0.09930415590186925 1103 44-45 0.1742366572768006 1103 46-47 0.20028645063631245 1103 48-49 -0.0083729121521543 1103 50-51 -0.20491194122856626 1103 52-53 -0.19477580720114673 1103 54-55 -0.10900474670028615 1103 56-57 -0.07318452586181223 1103 58-59 -0.00756062696366655 1103 60-61 -0.0647014172335858 1103 62-63 -0.2171287148858596 1103 64-65 -0.2558731476139755 1103 66-67 -0.1024535300630589 1103 68-69 0.2105851682944433 1103 70-71 -0.07756906702808664 1103 72-73 0.07109327318781311 1103 74-75 0.15817647457493678 1103 76-77 -0.25177245643335056 1103 78-79 -0.14142207769771886 1103 80-81 -0.1532700418362154 1103 82-83 -0.07534617746845385 1103 84-85 0.015729688513324902 1103 86-87 -0.07364376269859463 1103 88-89 -0.11750131146487774 1103 90-91 -0.006814995885935815 1103 92-93 0.046697947943016516 1103 94-95 0.0978400000606996 1103 96-97 -8.143331461312187E-4 1103 98-99 -0.13440816717099224 1103 100-101 0.07380700880183966 1104 1 -0.04830006017828481 1104 2 -0.0014784009049435554 1104 3 -0.06315998476649298 1104 4 0.015978287698473537 1104 5 0.004665891782224207 1104 6 0.017677523508353943 1104 7 0.005029396221139848 1104 8 -0.06586776978674891 1104 9 -0.09243516562877829 1104 10-11 5.387502432014912E-4 1104 12-13 0.12619595515804605 1104 14-15 0.06749938439515546 1104 16-17 0.09911891212957613 1104 18-19 0.12230902250207265 1104 20-21 -0.0026466864668321932 1104 22-23 -0.06987873084371898 1104 24-25 0.011149334519842569 1104 26-27 -0.020294725195434182 1104 28-29 0.008929454574413853 1104 30-31 -0.01011079870342968 1104 32-33 -0.11005775110395177 1104 34-35 -0.047390892954062735 1104 36-37 0.014191983965297084 1104 38-39 0.04441106526464722 1104 40-41 0.06542289717353356 1104 42-43 -0.18809694488933104 1104 44-45 -0.16380513838883815 1104 46-47 -0.014918227726269606 1104 48-49 0.02019175933047279 1104 50-51 0.07217354208756888 1104 52-53 -0.0014794535719815372 1104 54-55 -0.005156277488040928 1104 56-57 0.09224375071197954 1104 58-59 0.17354532418872282 1104 60-61 0.039486749261087084 1104 62-63 0.2830045840477524 1104 64-65 -0.1476903130766516 1104 66-67 -0.17560024488933834 1104 68-69 0.16311354806740752 1104 70-71 -0.1817314337291478 1104 72-73 -0.105871811091518 1104 74-75 0.0027757177809917266 1104 76-77 0.011148940195475632 1104 78-79 -0.011158919802987555 1104 80-81 -0.07499346804880958 1104 82-83 -0.038375073237283175 1104 84-85 -0.03285992276978078 1104 86-87 0.02649383620061485 1104 88-89 -0.09073402560316524 1104 90-91 -0.05182703578961778 1104 92-93 0.08338955441016083 1104 94-95 0.18924006886014766 1104 96-97 0.00427252615899576 1104 98-99 0.03934054283932653 1104 100-101 0.21588217220053352 1105 1 -0.005643889903481636 1105 2 0.003016581722555145 1105 3 -0.02010048091538863 1105 4 0.014559331020386423 1105 5 -0.04399168773037587 1105 6 0.03418018405212564 1105 7 0.05894974998842173 1105 8 0.09945040209166933 1105 9 0.11451383454130593 1105 10-11 0.2094776913710774 1105 12-13 0.057772600686149644 1105 14-15 0.19055743041405293 1105 16-17 0.10372809161301433 1105 18-19 0.10067722232226828 1105 20-21 0.05916053952145717 1105 22-23 0.02305564240990776 1105 24-25 0.053008556523394645 1105 26-27 0.08740505855946168 1105 28-29 0.15931699496851337 1105 30-31 0.20392326597572463 1105 32-33 0.30099747643934904 1105 34-35 0.30472660832153764 1105 36-37 0.32170467925407564 1105 38-39 0.38436295680192956 1105 40-41 0.301834686176619 1105 42-43 0.3382120669434059 1105 44-45 0.261896443359106 1105 46-47 0.37421849263722606 1105 48-49 0.4482671778498002 1105 50-51 0.4236155811088764 1105 52-53 0.45701363387365745 1105 54-55 0.3800588742480002 1105 56-57 0.43226124469842375 1105 58-59 0.4175111137263414 1105 60-61 0.3819120961036617 1105 62-63 0.4374667654506723 1105 64-65 0.4731761251962965 1105 66-67 0.5508326826806993 1105 68-69 0.5792736180433522 1105 70-71 0.29723690701483463 1105 72-73 0.5075791207062395 1105 74-75 0.4946240060430114 1105 76-77 0.41188028601757054 1105 78-79 0.3555077468636796 1105 80-81 0.3121411627551893 1105 82-83 0.31964664846254465 1105 84-85 0.38845844348626457 1105 86-87 0.2927620287316586 1105 88-89 0.3721933014614862 1105 90-91 0.4525634688632927 1105 92-93 0.5121865998702297 1105 94-95 0.2365734507878905 1105 96-97 0.1779708034872769 1105 98-99 0.3015608212852783 1105 100-101 0.7497208873644219 1106 1 -0.09843331736465899 1106 2 -0.03698985467739391 1106 3 -0.08924666539343917 1106 4 -0.07462390787231499 1106 5 -0.057739717710767025 1106 6 -0.016079377310227017 1106 7 -0.031051573383535924 1106 8 -0.01035187410808902 1106 9 -0.13778702805659293 1106 10-11 0.07513739228901528 1106 12-13 -0.11144682039877551 1106 14-15 0.039663703197113875 1106 16-17 -0.09549051740275871 1106 18-19 0.0021213519645968404 1106 20-21 0.09452239152035702 1106 22-23 0.022567649002716905 1106 24-25 0.018461029424344133 1106 26-27 -0.1189161478745362 1106 28-29 -0.08333528303584359 1106 30-31 -0.003297231852748439 1106 32-33 -0.12284171582253123 1106 34-35 0.041313180908481684 1106 36-37 -0.08664880542488618 1106 38-39 0.03600634415290216 1106 40-41 -0.17304004463749578 1106 42-43 -0.22561280884008994 1106 44-45 -0.10416778830317241 1106 46-47 -0.07700021262378698 1106 48-49 -0.22572089375665172 1106 50-51 -0.1871679453164532 1106 52-53 -0.19203564368556414 1106 54-55 -0.0972520640227188 1106 56-57 -0.12540092851893547 1106 58-59 -0.21291320744399655 1106 60-61 -0.23204301151053386 1106 62-63 -0.18447669649035703 1106 64-65 -0.10462761874325821 1106 66-67 -0.24972614120255088 1106 68-69 -0.2779368041123007 1106 70-71 -0.2692655412232767 1106 72-73 -0.30723452446164856 1106 74-75 -0.3038189265492335 1106 76-77 -0.35817255863059216 1106 78-79 -0.30873173533696985 1106 80-81 -0.2201581358576803 1106 82-83 -0.20966528835246123 1106 84-85 -0.23474297372479214 1106 86-87 -0.09579501461479367 1106 88-89 -0.0677376425839391 1106 90-91 -0.0838270040617175 1106 92-93 -0.007447427631845471 1106 94-95 -0.12074073575943345 1106 96-97 -0.12313165557842254 1106 98-99 -0.013476894815347862 1106 100-101 0.4212584651125191 1107 1 0.042833784663997676 1107 2 0.008893863943022495 1107 3 0.0477806233608149 1107 4 0.014045920548710455 1107 5 0.03004241920478279 1107 6 0.007276841496413056 1107 7 0.14410299667581228 1107 8 0.08545362833771719 1107 9 0.004268443351051587 1107 10-11 -0.06230580786989748 1107 12-13 -0.023548290366250058 1107 14-15 0.046087051345992336 1107 16-17 0.028686971237277703 1107 18-19 0.08640393582971484 1107 20-21 0.0979784911404451 1107 22-23 -0.05878182831460066 1107 24-25 0.04854632230046718 1107 26-27 0.11055210629078971 1107 28-29 0.11393087543261515 1107 30-31 0.005731770208193154 1107 32-33 0.14473054102448657 1107 34-35 0.04075914967167904 1107 36-37 -0.017464736693973748 1107 38-39 0.05110330737888802 1107 40-41 -0.09158193374433665 1107 42-43 -0.09621004520192145 1107 44-45 0.12468591020451925 1107 46-47 -0.001093277456305941 1107 48-49 -0.12570196047628457 1107 50-51 0.02254296521913801 1107 52-53 0.081612333867632 1107 54-55 0.09042485351508134 1107 56-57 -0.008438261223226107 1107 58-59 -0.008452402438159368 1107 60-61 0.13337705900817554 1107 62-63 0.1310153825700624 1107 64-65 0.12717470539450204 1107 66-67 0.045868922487763086 1107 68-69 0.031698373301850324 1107 70-71 0.14543537502333237 1107 72-73 0.04633051911592645 1107 74-75 0.031477053387696685 1107 76-77 -0.014057084243283668 1107 78-79 -0.017680658933421967 1107 80-81 0.2766263102973099 1107 82-83 0.24159082616595384 1107 84-85 0.06131455861697788 1107 86-87 0.09088712974991608 1107 88-89 0.2511670826662282 1107 90-91 -0.06258293235114465 1107 92-93 0.15578227961619007 1107 94-95 0.2805159995222688 1107 96-97 -0.11101590805527195 1107 98-99 -0.11364325937721276 1107 100-101 0.16910752031079213 1108 1 -0.16549375846685166 1108 2 -0.1320386789660759 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2316 64-65 -0.6483787116415058 2316 66-67 -0.7545900135976567 2316 68-69 -0.6325257413210181 2316 70-71 -0.64871031089735 2316 72-73 -0.7549170099593709 2316 74-75 -0.7273797221031302 2316 76-77 -0.628244347790563 2316 78-79 -0.5488253254412001 2316 80-81 -0.5761711670062297 2316 82-83 -0.70388860833026 2316 84-85 -0.5839467735894601 2316 86-87 -0.8578378030068805 2316 88-89 -0.9401175378177626 2316 90-91 -1.002975816889112 2316 92-93 -1.005656975190977 2316 94-95 -0.6946135300056575 2316 96-97 -0.389165423283373 2316 98-99 -0.5979189655059045 2316 100-101 -0.5216608846985515 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 5.0 5 7.0 6 29.0 7 66.0 8 148.0 9 234.0 10 408.0 11 705.0 12 1240.0 13 1931.0 14 2236.0 15 3267.0 16 4365.0 17 6492.0 18 5852.0 19 2918.0 20 2180.0 21 2160.0 22 2402.0 23 2823.0 24 3413.0 25 3941.0 26 4854.0 27 5913.0 28 7795.0 29 9564.0 30 12303.0 31 15460.0 32 19467.0 33 25411.0 34 34382.0 35 57131.0 36 79108.0 37 174551.0 38 339509.0 39 81243.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.414571170747884 24.063970350919178 15.01489699447373 21.50656148385921 2 13.53824626559265 24.05305415013834 41.920837024036196 20.487862560232816 3 15.267217353419607 31.850106072514627 31.516234896434252 21.366441677631506 4 9.591188428134503 20.08481422532035 43.912816931318865 26.411180415226276 5 9.035429752409893 42.70989550379519 37.7192329501295 10.535441793665424 6 24.249665581494124 44.44088071932271 19.696538467432784 11.612915231750383 7 24.894748480065154 35.20698918257389 25.589091060352963 14.309171277007989 8 23.455490046260312 37.73466765040075 22.784573181277818 16.02526912206111 9 26.60570142502786 16.624814181533967 24.170302760950428 32.59918163248774 10-11 21.383185836348677 29.968916987722782 31.013117917859624 17.63477925806891 12-13 21.937032990850835 29.162224173148875 30.421192171870317 18.479550664129977 14-15 20.732579039895963 30.557039427449244 27.834347353003636 20.876034179651153 16-17 17.360137314851 33.59988440331421 29.629805992418344 19.410172289416444 18-19 17.017359111836992 33.0143312834008 32.772739911069856 17.195569693692352 20-21 18.110496094526095 32.170749475520935 32.681189523445155 17.03756490650781 22-23 18.53559723532773 31.23988808200314 32.338050586409146 17.886464096259985 24-25 17.98003726319154 31.96440730288494 32.67923879750674 17.376316636416778 26-27 17.130604459210566 32.614196458137776 32.68141028623348 17.57378879641818 28-29 17.72115663025172 30.479962473939477 33.833607827489594 17.965273068319217 30-31 17.711824887193846 31.90356141230457 33.06314955216888 17.3214641483327 32-33 16.92140711966954 33.16389558314263 32.566256691897145 17.348440605290683 34-35 17.282624163939094 31.80392113934166 33.36225109741547 17.551203599303783 36-37 17.86162509968249 31.110297413701094 32.13172483964596 18.896352646970445 38-39 17.140408231625717 32.700948139926034 32.93318029983837 17.225463328609873 40-41 16.62269750273393 31.977933682824332 34.18883460551997 17.210534208921764 42-43 17.23246315508308 31.761013518469255 32.28132515810227 18.725198168345393 44-45 17.011549869810125 32.1864837896228 32.694106616119676 18.107859724447398 46-47 17.25894188044575 31.254471692917935 33.12482311666117 18.361763309975153 48-49 17.08878810356012 32.25176746872203 32.47478466031482 18.184659767403033 50-51 17.837279225538488 31.305239799641036 31.722225536845873 19.135255437974603 52-53 17.314165010402853 31.88477987063353 32.46007040330001 18.34098471566361 54-55 18.369632324930706 31.613893012115717 32.00966805469807 18.006806608255502 56-57 18.05532597332224 32.20351507123739 31.111281397270922 18.629877558169447 58-59 17.457537317680853 31.307717064327846 32.54399523594758 18.690750382043724 60-61 17.99508050285274 33.712731579800064 30.49874768474934 17.793440232597852 62-63 17.720349502915077 33.13939614198173 32.2093186181893 16.93093573691389 64-65 17.75458419584048 31.287800253744035 32.26834909837672 18.689266452038762 66-67 18.0186442422 32.56557558296097 31.039455910395546 18.376324264443486 68-69 17.575526038667753 31.962089748950767 31.895643911612805 18.566740300768668 70-71 18.330279226380476 30.78523388548084 33.03416221139236 17.850324676746315 72-73 18.054680568425695 31.794937195233086 31.330189035533994 18.82019320080722 74-75 17.70480533668445 32.199795079724055 31.18679709170839 18.9086024918831 76-77 17.472779335055083 31.49524032557851 32.774761029079066 18.257219310287333 78-79 16.711597828838574 31.642003842162307 32.52024368032245 19.126154648676668 80-81 17.727002044846504 32.57988913166272 30.639583762079702 19.053525061411076 82-83 18.825134697863007 31.13556702272294 31.252586133290134 18.78671214612392 84-85 16.84714073036168 31.492195025834135 31.671775111656014 19.988889132148174 86-87 17.56053234594046 31.876797308784433 31.764005649448123 18.798664695826982 88-89 18.17110431164185 31.18010075844188 31.88716111878438 18.76163381113189 90-91 17.304786170843673 30.671854064620995 32.896138555143104 19.12722120939223 92-93 18.367289798127807 32.01599342080341 31.301746528545994 18.314970252522787 94-95 17.532869512101005 30.029660694571476 32.401093159977826 20.036376633349697 96-97 18.349191788049893 30.43279788242558 31.559784476813302 19.658225852711226 98-99 17.34700970719679 31.05000287357108 31.835445636140218 19.767541783091907 100-101 17.31355194229783 30.556305099458854 32.123870507274894 20.006272450968428 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1332.0 1 861.5 2 812.5 3 1270.0 4 951.0 5 1132.5 6 2814.0 7 4577.0 8 5210.5 9 4596.0 10 3186.5 11 2278.5 12 2103.5 13 1969.0 14 1761.0 15 1545.5 16 1380.5 17 1347.5 18 1376.5 19 1382.0 20 1508.0 21 1689.0 22 1898.0 23 2378.0 24 2569.0 25 2988.5 26 5259.5 27 7705.5 28 7873.0 29 9660.5 30 16197.5 31 22249.5 32 30986.0 33 41917.0 34 49567.0 35 57014.5 36 67908.5 37 73969.0 38 67640.0 39 61535.0 40 54938.0 41 45987.5 42 36097.5 43 34203.0 44 35922.5 45 31821.5 46 24479.0 47 17054.5 48 15818.5 49 13153.0 50 10543.0 51 7115.0 52 3398.5 53 2347.5 54 1504.5 55 1149.5 56 918.0 57 690.0 58 547.5 59 410.5 60 310.0 61 305.0 62 284.0 63 211.5 64 137.5 65 78.5 66 53.0 67 62.0 68 62.5 69 55.5 70 41.0 71 22.0 72 17.0 73 14.5 74 9.0 75 4.0 76 4.5 77 6.0 78 4.5 79 3.5 80 2.0 81 1.0 82 2.0 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2850493582010802 2 0.02134586207726983 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.925968171677653E-4 12-13 0.0 14-15 0.0 16-17 5.473297968530726E-4 18-19 0.0 20-21 5.473297968530726E-5 22-23 0.0 24-25 0.0026271830248947485 26-27 0.0025724500452094412 28-29 0.0030103138826918993 30-31 0.0013135915124473743 32-33 0.0015872564108739104 34-35 0.0017514553499298324 36-37 5.473297968530726E-5 38-39 5.473297968530726E-5 40-41 7.662617155943015E-4 42-43 0.003831308577971508 44-45 0.0057469628669572625 46-47 0.015489433250941953 48-49 0.015708365169683183 50-51 0.007170020338775252 52-53 0.008155213973110783 54-55 2.1893191874122904E-4 56-57 0.0033934447404890505 58-59 0.001970387268671061 60-61 0.001970387268671061 62-63 0.0 64-65 7.662617155943015E-4 66-67 0.0 68-69 0.0 70-71 2.1893191874122904E-4 72-73 0.0022440521670975978 74-75 0.0 76-77 0.20322355357154584 78-79 0.1115458125986562 80-81 0.0010946595937061452 82-83 0.0 84-85 6.567957562236871E-4 86-87 0.1340410672493175 88-89 0.6143229639878887 90-91 0.326865354680655 92-93 0.3039322361925112 94-95 0.0030103138826918993 96-97 0.003065046862377206 98-99 0.003119779842062514 100-101 8.209946952796089E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 913526.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.575944198632552 #Duplication Level Percentage of deduplicated Percentage of total 1 66.85263005361541 7.070296849788621 2 10.74067940464115 2.2718565207777335 3 4.517978760842114 1.433456737958197 4 2.6165980085701865 1.106919781155654 5 1.7751050572380813 0.9386706016030194 6 1.3300349845778043 0.843982546747438 7 1.023661167118637 0.7578328367227642 8 0.8094065042333408 0.6848190418225645 9 0.6541495021425466 0.6226423769000554 >10 6.514583807729729 15.032303404610268 >50 1.3590162916347526 10.231564290452598 >100 1.5618854410333904 34.76058700026053 >500 0.18527335582834786 13.193056355265204 >1k 0.05796261411389654 10.279838778535039 >5k 0.0010350466806052954 0.7721728774003148 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7054 0.7721728774003148 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4855 0.5314572327443334 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4075 0.4460737844352542 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 2995 0.3278505483149905 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2926 0.3202973971184181 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2873 0.3144957012717755 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 2443 0.2674253387424113 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 2411 0.2639224280425516 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 2287 0.2503486490805954 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2271 0.24859719373066558 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2101 0.22998798063766113 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 2082 0.2279081274096194 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2064 0.22593774014094836 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1886 0.206452799372979 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1869 0.20459187806367857 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1838 0.20119843332318949 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1777 0.194521009801582 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1763 0.1929884863703934 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 1753 0.19189382677668726 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1710 0.18718679052375084 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1696 0.18565426709256222 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1696 0.18565426709256222 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1679 0.18379334578326179 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1656 0.18127562871773764 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1614 0.17667805842417184 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1613 0.17656859246480122 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 1547 0.16934383914634066 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1535 0.1680302476338933 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 1486 0.1626664156247332 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1412 0.1545659346313077 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1402 0.15347127503760155 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 1388 0.15193875160641296 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 1384 0.1515008877689305 No Hit GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 1373 0.15029676221585375 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1342 0.1469033174753647 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1308 0.1431814748567638 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1304 0.14274361101928132 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1302 0.1425246791005401 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1278 0.13989749607564536 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 1237 0.13540939174145017 No Hit GGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATAC 1184 0.12960769589480758 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1180 0.12916983205732513 No Hit ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT 1163 0.1273089107480247 No Hit TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA 1153 0.12621425115431853 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 1153 0.12621425115431853 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1147 0.12555745539809485 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 1140 0.12479119368250055 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1127 0.12336813621068256 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1087 0.11898949783585798 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1079 0.11811377016089307 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1074 0.11756644036403999 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1067 0.11680017864844569 No Hit GTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAAT 1035 0.11329726794858602 No Hit GGCATTAGCAGTCCGGCTTACAGCTAACATTACTGCAGGACACTTATTAA 1033 0.1130783360298448 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 1032 0.11296887007047418 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1019 0.1115458125986562 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 1005 0.1100132891674676 No Hit GCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGCCATTGGTTGAATAAA 992 0.10859023169564962 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 988 0.10815236785816713 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 971 0.1062914465488667 No Hit CAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTAC 964 0.1055251848332724 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 953 0.10432105928019564 No Hit AAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAGGCTTCCGAC 951 0.10410212736145441 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 951 0.10410212736145441 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 936 0.1024601379708952 No Hit GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG 933 0.10213174009278336 No Hit CTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTCC 927 0.10147494433655965 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 923 0.1010370804990772 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 918 0.10048975070222413 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.0946595937061452E-4 0.0 16-17 0.0 0.0 0.0 8.757276749649162E-4 0.0 18-19 0.0 0.0 0.0 0.001477790451503296 0.0 20-21 0.0 0.0 0.0 0.0026271830248947485 0.0 22-23 0.0 0.0 0.0 0.0057469628669572625 0.0 24-25 0.0 0.0 0.0 0.008812009729334469 0.0 26-27 0.0 0.0 0.0 0.01116552785580268 0.0 28-29 0.0 0.0 0.0 0.014394773657235809 0.0 30-31 0.0 0.0 0.0 0.021455328036640443 0.0 32-33 0.0 0.0 0.0 0.03311345270961089 0.0 34-35 0.0 0.0 0.0 0.05101113706670636 0.0 36-37 0.0 0.0 0.0 0.07980068438117799 0.0 38-39 0.0 0.0 0.0 0.12544798943872426 0.0 40-41 0.0 0.0 0.0 0.17974310528654905 0.0 42-43 0.0 0.0 0.0 0.22987851467829049 0.0 44-45 0.0 0.0 0.0 0.2809443847246822 0.0 46-47 0.0 0.0 0.0 0.3511667976609314 0.0 48-49 0.0 0.0 0.0 0.4274645713422497 0.0 50-51 0.0 0.0 0.0 0.5015730258361557 0.0 52-53 0.0 0.0 0.0 0.5791843910299215 0.0 54-55 0.0 0.0 0.0 0.6609554626797705 0.0 56-57 0.0 0.0 0.0 0.7584348994993027 0.0 58-59 0.0 0.0 0.0 0.8688313195245674 0.0 60-61 0.0 0.0 0.0 0.9852483673152159 0.0 62-63 0.0 0.0 0.0 1.096575247995131 0.0 64-65 0.0 0.0 0.0 1.219505520368331 0.0 66-67 0.0 0.0 0.0 1.362577529265724 0.0 68-69 0.0 0.0 0.0 1.5159393383439552 0.0 70-71 0.0 0.0 0.0 1.6933836584837212 0.0 72-73 0.0 0.0 0.0 1.8679818636798515 0.0 74-75 0.0 0.0 0.0 2.0412664773635343 0.0 76-77 0.0 0.0 0.0 2.241479717052388 0.0 78-79 0.0 0.0 0.0 2.474806409450853 0.0 80-81 0.0 0.0 0.0 2.7399329630464813 0.0 82-83 0.0 0.0 0.0 3.002651265535956 0.0 84-85 0.0 0.0 0.0 3.260771997731865 0.0 86-87 0.0 0.0 0.0 3.54576662295326 0.0 88-89 0.0 0.0 0.0 3.8292287247434666 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 30 1.3777644E-6 79.06348 2 TCACGCA 30 1.3791105E-6 79.05049 4 ACAGTCA 20 0.0020860555 71.14545 8 GGTAACG 20 0.0020860555 71.14545 8 GTAACGA 20 0.0020860555 71.14545 9 TATCACG 35 3.4430104E-6 67.76869 2 ATCACGC 35 3.4463683E-6 67.75757 3 GTATGGT 70 1.8189894E-11 61.192818 1 GGGCGAC 40 7.6102006E-6 59.28787 7 GGCGACT 40 7.6102006E-6 59.28787 8 CTCTGTA 25 0.0050469344 56.9257 2 CTGACCA 25 0.0050502163 56.916355 9 TACAGTC 25 0.0050502163 56.916355 7 CACGCAG 45 1.528955E-5 52.700325 5 GTATCAC 50 2.7935515E-5 47.594414 1 TACAGGG 70 8.000279E-8 47.438087 2 TAACGCA 50 2.8511746E-5 47.430294 4 TCAGGCC 50 2.8511746E-5 47.430294 3 TGGGCGA 100 1.2732926E-11 47.430294 6 ATGGGCG 115 1.8189894E-12 45.368107 5 >>END_MODULE