FastQCFastQC Report
Thu 26 May 2016
SRR936335_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936335_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793524
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80261.011437587268942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57380.7231035230188375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43230.5447850348571688No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18120.2283484809533171No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16960.21373014552804956No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16300.20541281675160425No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15400.19407100478372424No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG14710.1853756156083496No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14120.17794042776273938No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.15916342794924918No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11540.14542723345481673No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT8820.11114975728522389No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8590.10825129422676566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG3250.036.436931
TACGGGA600.004010576331.568722
CATATAG1401.7578714E-830.439353
GTACATG81250.030.2572251
GTATCAA83100.029.982641
GGTATCA56200.029.331081
TACATGG82450.029.2905672
ATGGGAT18700.029.1190435
ACATGGG82350.028.2893123
ATACCGT700.00852327.0554926
TACCGTC700.00852327.0554927
TACCTGG6850.026.9601482
CATGGGA56050.025.7658884
ATGGGTA5150.025.743745
TAGCCCT4900.025.1245424
GTGTACG1151.3056968E-424.7137451
ATCAACG100800.024.4736123
TCAACGC100650.024.4630414
CATGGGG22200.024.1016254
CAACGCA102350.024.0104565
Produced by FastQC (version 0.11.5)