##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936335_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 793524 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.779016135617827 33.0 31.0 34.0 30.0 34.0 2 31.836945070344438 33.0 31.0 34.0 30.0 34.0 3 31.88920435928844 34.0 31.0 34.0 30.0 34.0 4 35.41397235622363 37.0 35.0 37.0 33.0 37.0 5 35.4307204318962 37.0 35.0 37.0 33.0 37.0 6 35.55045720104244 37.0 35.0 37.0 33.0 37.0 7 35.50104092629839 37.0 35.0 37.0 33.0 37.0 8 35.502452351787724 37.0 35.0 37.0 33.0 37.0 9 37.15504256960092 39.0 37.0 39.0 34.0 39.0 10-11 37.0770020818526 39.0 37.0 39.0 33.5 39.0 12-13 36.9810850081409 39.0 37.0 39.0 33.0 39.0 14-15 38.11952379512151 40.0 38.0 41.0 33.0 41.0 16-17 38.097725462619906 40.0 38.0 41.0 33.0 41.0 18-19 38.14024024478151 40.0 38.0 41.0 33.0 41.0 20-21 38.09444704886053 40.0 38.0 41.0 33.0 41.0 22-23 37.929821908348075 40.0 38.0 41.0 33.0 41.0 24-25 38.04800421915405 40.0 38.0 41.0 33.0 41.0 26-27 37.96516740514464 40.0 38.0 41.0 33.0 41.0 28-29 37.80515649180113 40.0 38.0 41.0 33.0 41.0 30-31 37.55775817744643 40.0 37.0 41.0 33.0 41.0 32-33 37.25982641986884 40.0 36.0 41.0 32.0 41.0 34-35 36.939191505234874 40.0 35.5 41.0 31.5 41.0 36-37 36.690440364752675 40.0 35.0 41.0 31.0 41.0 38-39 36.48386312197236 40.0 35.0 41.0 30.5 41.0 40-41 36.241379592803746 39.5 35.0 41.0 30.0 41.0 42-43 35.97527106930603 39.0 35.0 41.0 29.5 41.0 44-45 35.66720099203049 39.0 35.0 41.0 28.5 41.0 46-47 35.19332559569717 39.0 35.0 41.0 25.5 41.0 48-49 34.86866748831794 39.0 35.0 41.0 24.0 41.0 50-51 34.48735829036047 38.5 34.5 40.5 23.5 41.0 52-53 34.466102474531326 38.5 34.5 40.0 23.0 41.0 54-55 34.52787313301173 39.0 35.0 41.0 23.0 41.0 56-57 34.38151020006956 38.0 35.0 41.0 22.5 41.0 58-59 34.160624631391116 38.0 34.0 40.5 22.0 41.0 60-61 33.887064537430504 37.5 34.0 40.0 20.5 41.0 62-63 33.58643846940987 37.0 34.0 40.0 20.0 41.0 64-65 33.27154566213498 36.5 34.0 40.0 18.0 41.0 66-67 32.93373924418165 36.0 33.0 39.5 16.5 41.0 68-69 32.58592619756932 35.5 33.0 39.0 12.5 41.0 70-71 32.20872588604756 35.0 33.0 39.0 8.0 40.5 72-73 31.83080032866051 35.0 32.5 37.5 7.0 40.0 74-75 31.432569147246966 35.0 32.0 37.0 2.0 39.5 76-77 31.051314768047344 35.0 32.0 36.5 2.0 39.0 78-79 30.66959663475837 35.0 31.5 36.0 2.0 39.0 80-81 30.300914654125144 35.0 31.0 36.0 2.0 37.5 82-83 29.972301404872443 35.0 31.0 35.0 2.0 37.0 84-85 29.653470468442038 35.0 31.0 35.0 2.0 36.5 86-87 29.32780356989833 34.0 30.0 35.0 2.0 36.0 88-89 28.992740610239892 34.0 30.0 35.0 2.0 36.0 90-91 28.625586623719006 34.0 29.0 35.0 2.0 35.5 92-93 28.323865440742814 34.0 29.0 35.0 2.0 35.0 94-95 27.963303567377924 34.0 29.0 35.0 2.0 35.0 96-97 27.563778789299377 34.0 28.5 35.0 2.0 35.0 98-99 27.090056507427626 34.0 27.0 35.0 2.0 35.0 100-101 24.03261338031364 30.0 13.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20686209654938637 1101 2 0.20286799439005065 1101 3 0.2244968708050159 1101 4 0.091112603229206 1101 5 0.13800940951576024 1101 6 0.1250628681576913 1101 7 0.19443887901821455 1101 8 -0.0029057186794076983 1101 9 0.01592663561601171 1101 10-11 0.2836983628644063 1101 12-13 0.19914740526298402 1101 14-15 0.16681021092401238 1101 16-17 -0.07144511963371514 1101 18-19 0.2985742481059006 1101 20-21 0.24891450862222086 1101 22-23 0.2130986333001914 1101 24-25 0.34546114726420996 1101 26-27 0.12869146231727058 1101 28-29 0.09463153340023212 1101 30-31 0.34277003071654377 1101 32-33 0.5652887156905777 1101 34-35 0.19548719525225522 1101 36-37 0.16854580671780894 1101 38-39 0.3944747249443097 1101 40-41 0.3690606420377591 1101 42-43 0.3880763163891885 1101 44-45 0.3090091112368327 1101 46-47 0.35549132973462605 1101 48-49 0.09365923872876891 1101 50-51 0.2702525389491015 1101 52-53 0.15681211722628774 1101 54-55 0.19318047971686525 1101 56-57 0.20015715235507514 1101 58-59 0.365149907067142 1101 60-61 0.14216325293415366 1101 62-63 -0.008186935797802164 1101 64-65 0.2733575538813824 1101 66-67 0.21886795775990464 1101 68-69 0.32702433802366215 1101 70-71 0.039598500780826384 1101 72-73 0.015640784917476225 1101 74-75 0.01654055252527087 1101 76-77 -0.055708519792638356 1101 78-79 -0.052304983789937864 1101 80-81 0.10834167013751994 1101 82-83 0.3478454624445604 1101 84-85 0.25179081916119017 1101 86-87 0.35980844471358253 1101 88-89 0.08622317546207725 1101 90-91 0.19837286145898148 1101 92-93 0.36842830083228506 1101 94-95 0.4992881267551965 1101 96-97 0.263273064049681 1101 98-99 0.4597545098446396 1101 100-101 0.7422328080028961 1102 1 0.4396446019916951 1102 2 0.36317780134541877 1102 3 0.2114803009905053 1102 4 0.17693020933178616 1102 5 0.3189640179391944 1102 6 0.2901043376717567 1102 7 0.22469993005899624 1102 8 0.23580935607366627 1102 9 0.32252217741836375 1102 10-11 0.39423575739150607 1102 12-13 0.37663422677315594 1102 14-15 0.5080611329685212 1102 16-17 0.4371298943093933 1102 18-19 0.5653352181866822 1102 20-21 0.5769329675035308 1102 22-23 0.4514201524697228 1102 24-25 0.4359586914773672 1102 26-27 0.347742251227551 1102 28-29 0.3622538279867058 1102 30-31 0.32979394090491354 1102 32-33 0.07858189885808997 1102 34-35 0.1914526882986891 1102 36-37 0.32907150702185817 1102 38-39 0.24097230513968526 1102 40-41 0.23423299689445543 1102 42-43 0.25168119557886826 1102 44-45 -0.0481482030499123 1102 46-47 0.04754420561393857 1102 48-49 0.20018236630375696 1102 50-51 0.1372060139324489 1102 52-53 0.21707334817819657 1102 54-55 0.4326781678589242 1102 56-57 0.47048486046726623 1102 58-59 0.24184332052089275 1102 60-61 0.21953403176941322 1102 62-63 0.24714967791502573 1102 64-65 0.10685430648559446 1102 66-67 0.22511986931559846 1102 68-69 0.29358560675691336 1102 70-71 0.197371560889124 1102 72-73 0.23178376028764447 1102 74-75 0.34372917134757586 1102 76-77 0.29931234989773614 1102 78-79 0.24490369512268018 1102 80-81 0.2767272376671599 1102 82-83 0.3894858920723223 1102 84-85 0.3280124156925055 1102 86-87 0.1717788313425892 1102 88-89 0.16801751905634532 1102 90-91 0.2571025989686504 1102 92-93 0.17953961184464262 1102 94-95 0.3230134564423288 1102 96-97 0.197832641618362 1102 98-99 0.49956659267572334 1102 100-101 0.3867509160575189 1103 1 0.2895491777027246 1103 2 0.22790805317244534 1103 3 0.325989447757717 1103 4 0.3111526177477444 1103 5 0.3870643763621402 1103 6 0.2328497642881473 1103 7 0.313397970369941 1103 8 0.24798177147188483 1103 9 0.23896773902521318 1103 10-11 0.36173331359874084 1103 12-13 0.30565950257263097 1103 14-15 0.26967496716299877 1103 16-17 0.30401301119250945 1103 18-19 0.3814107197460572 1103 20-21 0.3427361607260835 1103 22-23 0.21477955292589712 1103 24-25 0.4019783349163646 1103 26-27 0.4822555873537837 1103 28-29 0.4403388254265366 1103 30-31 0.18991589780106466 1103 32-33 0.38256644477812074 1103 34-35 0.244750644822453 1103 36-37 0.21213020459668286 1103 38-39 0.1163970595967001 1103 40-41 0.1425791310470359 1103 42-43 0.04349332610067336 1103 44-45 0.07671797020336157 1103 46-47 0.13282093605195655 1103 48-49 0.2723557126706453 1103 50-51 0.18079862204118768 1103 52-53 0.14392248470475266 1103 54-55 0.19937741842914392 1103 56-57 0.10462580912435016 1103 58-59 0.2437983547339897 1103 60-61 0.1612321186287886 1103 62-63 0.18181895129396963 1103 64-65 0.25283019439697085 1103 66-67 0.3101289462601571 1103 68-69 0.42572497276724164 1103 70-71 0.3776769186138438 1103 72-73 0.28491879729364555 1103 74-75 0.21371939617650781 1103 76-77 0.051895787965040796 1103 78-79 0.26931834856959114 1103 80-81 0.26603273503717517 1103 82-83 0.39468767953217565 1103 84-85 0.42951127525588007 1103 86-87 0.36881835096275495 1103 88-89 0.3965470540374909 1103 90-91 0.5995128768628 1103 92-93 0.5838825807037402 1103 94-95 0.5928430891751901 1103 96-97 0.5832420350922725 1103 98-99 0.7397797845018168 1103 100-101 0.9390829925831419 1104 1 0.4035462916998398 1104 2 0.2945832080828943 1104 3 0.4302675226240247 1104 4 0.2983353696363764 1104 5 0.40493212952400626 1104 6 0.2996946840742467 1104 7 0.47565075026977865 1104 8 0.5118388294465888 1104 9 0.5141566024493684 1104 10-11 0.5643689132931655 1104 12-13 0.553622324064861 1104 14-15 0.5362058219291441 1104 16-17 0.7338576763312972 1104 18-19 0.392912290071159 1104 20-21 0.654466913633307 1104 22-23 0.6761792643256115 1104 24-25 0.5863854310881749 1104 26-27 0.41923037726975565 1104 28-29 0.6130321451786784 1104 30-31 0.4070239531444102 1104 32-33 0.3018008051889538 1104 34-35 0.16063214893924993 1104 36-37 0.46418860371390736 1104 38-39 0.36535724973336414 1104 40-41 0.45832057914142865 1104 42-43 0.4099095895757614 1104 44-45 0.22814823928068506 1104 46-47 0.06028889293168049 1104 48-49 -0.06076541456813089 1104 50-51 -0.10531885525275442 1104 52-53 -0.058543350114021564 1104 54-55 0.04406377782137838 1104 56-57 0.24336615374704706 1104 58-59 0.25175608327995036 1104 60-61 0.30498052708308165 1104 62-63 0.06453264283119609 1104 64-65 -0.00803475764445949 1104 66-67 0.19292752023520876 1104 68-69 0.0319765643129486 1104 70-71 -0.21739141174860777 1104 72-73 -0.15740358620520922 1104 74-75 -0.09243033644381171 1104 76-77 0.037508214753941616 1104 78-79 0.12202609833616407 1104 80-81 0.0931696504093864 1104 82-83 0.1482527419207642 1104 84-85 -0.1403681916824091 1104 86-87 -0.16893311325929616 1104 88-89 0.16640954448821432 1104 90-91 0.3759325356354033 1104 92-93 0.1756192753816137 1104 94-95 -0.05466136715279646 1104 96-97 -0.1409324831998937 1104 98-99 -0.17888161945370484 1104 100-101 0.15184082298803148 1105 1 -0.004792150973369047 1105 2 0.13250893631409966 1105 3 0.13488155707900873 1105 4 0.19582397881395508 1105 5 -0.005227642539324506 1105 6 -0.00453945593368843 1105 7 0.13144353948847254 1105 8 0.16823271747390578 1105 9 0.1277823102432194 1105 10-11 0.10838437355452157 1105 12-13 0.23823479566294026 1105 14-15 0.23566843959423522 1105 16-17 -0.09210017462024211 1105 18-19 0.32488593789938847 1105 20-21 0.22239107900761468 1105 22-23 0.43895261400882646 1105 24-25 0.327450097298005 1105 26-27 0.31180800918088636 1105 28-29 0.45783444378052707 1105 30-31 0.43333104250375953 1105 32-33 0.30361011260291093 1105 34-35 0.10462777293992076 1105 36-37 0.10574136215634411 1105 38-39 0.33017493646470797 1105 40-41 0.3911748367242893 1105 42-43 0.1664735885659212 1105 44-45 0.4560849075102027 1105 46-47 0.4198481672851955 1105 48-49 0.3227957352136741 1105 50-51 0.2251322092932284 1105 52-53 0.3738180064457737 1105 54-55 0.5105795686870849 1105 56-57 0.46434810876081656 1105 58-59 0.5097627194108014 1105 60-61 0.4695854248361755 1105 62-63 0.16792464340824864 1105 64-65 0.308927649636054 1105 66-67 0.25691749390707486 1105 68-69 0.20862447669679085 1105 70-71 0.21228603863068685 1105 72-73 0.26384044788911254 1105 74-75 0.40405624101979143 1105 76-77 0.3548703527421857 1105 78-79 0.22541208792513245 1105 80-81 0.21885340030419087 1105 82-83 0.3972609288865385 1105 84-85 0.31868195198020643 1105 86-87 0.38927428987362944 1105 88-89 0.4182849727711151 1105 90-91 0.4380019986137249 1105 92-93 0.35304260522857334 1105 94-95 0.169659691742865 1105 96-97 0.07545106592791129 1105 98-99 0.2819117107539135 1105 100-101 0.37231785500255654 1106 1 0.1474205404284561 1106 2 0.24705934416839526 1106 3 0.07995069686459644 1106 4 0.28960566416195377 1106 5 0.2853677989374006 1106 6 0.10243325523086355 1106 7 0.1619388577457812 1106 8 0.10908938015231229 1106 9 0.36977652031943364 1106 10-11 0.35421009248328517 1106 12-13 0.3598351004375431 1106 14-15 0.4230323972194583 1106 16-17 0.29575427963280987 1106 18-19 0.24842342656459238 1106 20-21 0.26012082568356476 1106 22-23 0.510783979998287 1106 24-25 0.3632204369351655 1106 26-27 0.2552560496746352 1106 28-29 0.2388650025934993 1106 30-31 0.29304146694604327 1106 32-33 0.2944172692503031 1106 34-35 0.1584157283904517 1106 36-37 0.32603625539594105 1106 38-39 0.37785351037354076 1106 40-41 0.42309580962197657 1106 42-43 0.541405877411286 1106 44-45 0.6086111040382391 1106 46-47 0.5557744658016048 1106 48-49 0.5701393745319763 1106 50-51 0.5815607509586584 1106 52-53 0.2152389783513442 1106 54-55 0.5493192780797358 1106 56-57 0.5892501384088717 1106 58-59 0.46651091725237137 1106 60-61 0.5419743537399597 1106 62-63 0.254186174146632 1106 64-65 0.3205461892974242 1106 66-67 0.29731004994611254 1106 68-69 0.4090604075589894 1106 70-71 0.5064262706641642 1106 72-73 0.5862259044259979 1106 74-75 0.5066626988285492 1106 76-77 0.5869901434477462 1106 78-79 0.7875029686959607 1106 80-81 0.7437502710071371 1106 82-83 0.5935697036569181 1106 84-85 0.8622085671764772 1106 86-87 0.8931742539594332 1106 88-89 0.6391394131347035 1106 90-91 0.6302361988482552 1106 92-93 0.5279417577726271 1106 94-95 0.5716814029649591 1106 96-97 0.6744383253176878 1106 98-99 0.7974788276377502 1106 100-101 0.61274751576223 1107 1 -0.012358334731104037 1107 2 0.1622750928026413 1107 3 0.1686058166898725 1107 4 0.12240269215571686 1107 5 0.0333134962954702 1107 6 0.18843282513437032 1107 7 0.07208420274038474 1107 8 0.28367501769145065 1107 9 0.12156941110057318 1107 10-11 0.4098819406947385 1107 12-13 0.2977650736255697 1107 14-15 0.4210012763840467 1107 16-17 0.13145939127046802 1107 18-19 0.3922907043102484 1107 20-21 0.4014815089451176 1107 22-23 0.42363913020652433 1107 24-25 0.3412973531827532 1107 26-27 0.3773649824631846 1107 28-29 0.46743564199704224 1107 30-31 0.41098556623914817 1107 32-33 0.4700712428092402 1107 34-35 0.26692729857805375 1107 36-37 0.15270440832878052 1107 38-39 0.18427457484263954 1107 40-41 0.3516080506022732 1107 42-43 0.09698598485648802 1107 44-45 0.27012121623818075 1107 46-47 0.31924112247214254 1107 48-49 0.30497206107646946 1107 50-51 0.38489814193018645 1107 52-53 0.6529243094960577 1107 54-55 0.531123039648449 1107 56-57 0.48311297708519874 1107 58-59 0.571463340293711 1107 60-61 0.613434491883794 1107 62-63 0.7548106032329969 1107 64-65 0.5610618710496951 1107 66-67 0.7103309254095009 1107 68-69 0.7127016281649503 1107 70-71 0.8003696044644215 1107 72-73 0.7549667673416174 1107 74-75 0.5922055378205471 1107 76-77 0.971476681230147 1107 78-79 0.9502323452730614 1107 80-81 0.8049650371273742 1107 82-83 0.8548552975945292 1107 84-85 0.8539548575942 1107 86-87 0.8164207722493835 1107 88-89 0.9638652266188217 1107 90-91 0.7031257173177465 1107 92-93 0.399299501383819 1107 94-95 0.5717307259575612 1107 96-97 0.8223461426174303 1107 98-99 0.9755555465933661 1107 100-101 0.8127936820833064 1108 1 -0.11349631481645517 1108 2 -0.022931166087829524 1108 3 -0.09597455315365266 1108 4 -0.1645460987404448 1108 5 -0.13293828179841682 1108 6 -0.1265031634858289 1108 7 -0.23517042030371016 1108 8 -0.13357249297711604 1108 9 -0.05492418776145058 1108 10-11 -0.14157609628961865 1108 12-13 -0.1308977997741465 1108 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-1.86232117200624 2316 50-51 -1.8171441259015566 2316 52-53 -2.0578052170229455 2316 54-55 -2.0396196955679713 2316 56-57 -1.935602731104197 2316 58-59 -2.1820674461318106 2316 60-61 -2.1577248253281525 2316 62-63 -2.006365986890966 2316 64-65 -2.047884481317709 2316 66-67 -2.1811545450233325 2316 68-69 -2.1467273496850794 2316 70-71 -1.8919693510324294 2316 72-73 -1.812118174743329 2316 74-75 -1.8182801358230236 2316 76-77 -2.0637295776603217 2316 78-79 -2.010979806728564 2316 80-81 -1.7281075091030687 2316 82-83 -1.8562044463599534 2316 84-85 -2.115792145047539 2316 86-87 -2.4808511237067172 2316 88-89 -2.2365259540355176 2316 90-91 -2.3579137055637815 2316 92-93 -2.455049208228047 2316 94-95 -2.6388463925142958 2316 96-97 -2.8667160398658034 2316 98-99 -2.7599910902690574 2316 100-101 -2.322163643663494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5555.0 3 1253.0 4 1120.0 5 1086.0 6 920.0 7 1084.0 8 1311.0 9 1528.0 10 1760.0 11 2385.0 12 3452.0 13 4976.0 14 5286.0 15 5789.0 16 7300.0 17 10184.0 18 8764.0 19 4921.0 20 4054.0 21 4135.0 22 4339.0 23 4603.0 24 5138.0 25 5999.0 26 6961.0 27 8505.0 28 9991.0 29 12006.0 30 14680.0 31 18322.0 32 23163.0 33 30722.0 34 43682.0 35 71699.0 36 106229.0 37 156110.0 38 161085.0 39 33367.0 40 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.460043899074996 18.468247554473557 12.724301958060119 21.34740658839133 2 15.913467840325882 20.364222551599664 40.34806457142281 23.374245036651644 3 19.928588803339412 27.671271550338844 28.235150289942567 24.164989356379177 4 11.787094286167985 15.58077224573334 37.17708619520642 35.45504727289225 5 12.819682296419154 40.00559585805677 31.969742035984893 15.204979809539173 6 30.952822044525103 35.54761589696088 17.98357876960497 15.515983288909052 7 29.53935379473569 34.08620607087136 19.44601975566293 16.92842037873002 8 28.552596257698788 32.97167249130086 19.27838390661765 19.197347344382703 9 29.871066130597534 15.241672233215281 19.999873964937045 34.88738767125014 10-11 26.045489373714563 27.058842803564946 25.400691111828046 21.49497671089245 12-13 27.373750740418668 24.530795115127226 26.996924899492104 21.098529244962002 14-15 24.176887114956962 25.15840303276824 26.51945608289701 24.145253769377785 16-17 21.829633196116934 28.981502254869586 27.079988000917577 22.1088765480959 18-19 21.586546581064084 26.260344275642332 31.347534892631273 20.80557425066231 20-21 22.792289380128366 25.401164204798217 31.067872459209354 20.738673955864062 22-23 21.89109057510957 25.6353995015486 30.765100840309657 21.708409083032173 24-25 21.42003205259499 25.96304309328339 31.49636966233858 21.12055519178304 26-27 21.527063609691176 26.18266235141103 31.307343496365302 20.98293054253249 28-29 21.072373421191607 25.727786397512475 31.95624143715835 21.243598744137564 30-31 21.166585582883172 26.331832653153825 31.639312443125817 20.862269320837186 32-33 20.64703109560588 26.263448042912742 31.463694065216075 21.6258267962653 34-35 21.320794650105665 26.308174888728136 31.386467951492104 20.9845625096741 36-37 21.77005626541475 25.91602222255985 30.859503588122983 21.45441792390242 38-39 21.607934911343733 26.086483140085825 30.983261307305636 21.322320641264806 40-41 21.420878512069645 26.273810842896715 31.209497427779976 21.09581321725366 42-43 21.273174351143492 26.466748715455306 30.704290088050506 21.555786845350696 44-45 21.054104607560184 26.821628165853422 30.330582280212298 21.793684946374093 46-47 21.492191007642763 27.00746798417621 29.499898995719825 22.000442012461203 48-49 21.7618440851771 27.13189733352924 29.08976223166833 22.016496349625335 50-51 22.13135364828668 27.121165769487288 28.79454940567012 21.95293117655591 52-53 21.988234690627078 27.504909600652038 28.6556655303287 21.851190178392184 54-55 21.935856026533425 27.333278034257148 28.802418300363897 21.928447638845533 56-57 21.909218809161697 27.789139637751543 28.03050353371433 22.271138019372426 58-59 21.79546679110124 27.023808242439745 28.686706626710844 22.494018339748173 60-61 22.056464224256366 27.407662531545228 28.742470613709088 21.793402630489314 62-63 22.28287467291678 27.260364256586456 28.688672490061673 21.768088580435087 64-65 22.11112011691789 27.168274625323257 28.750313444290505 21.970291813468354 66-67 22.08569771828939 27.679572980049276 28.210625868840257 22.024103432821082 68-69 22.28930158102967 27.50173502935619 28.487409005942162 21.721554383671982 70-71 21.923879007659203 27.339386580126597 28.710547008049186 22.026187404165015 72-73 22.05153011896388 27.65364030475115 28.12616540467916 22.16866417160582 74-75 21.82509577937498 28.05066016527879 28.021150619003894 22.103093436342334 76-77 21.831695768840646 27.742567010557323 28.576186321450596 21.84955089915144 78-79 22.06318202107756 27.482311376752637 28.41386471487681 22.040641887293 80-81 21.421945649069954 28.521036465486294 28.07920325657289 21.977814628870867 82-83 22.066546364010513 28.246240541995128 27.575015037832017 22.112198056162345 84-85 21.69321683719181 27.78374723064172 27.68300875620593 22.840027175960547 86-87 21.59787910197419 27.71325326989719 27.94069134335994 22.74817628476868 88-89 22.023046564293782 27.613510282040497 27.972647087135165 22.39079606653055 90-91 21.63347577607646 27.88839296460384 28.109088421343355 22.36904283797634 92-93 21.598250387620467 27.772879741383676 28.145996108594534 22.48287376240132 94-95 21.295238071117094 27.807709427804923 28.318746007032058 22.578306494045925 96-97 21.655734062694048 27.791516940736066 27.78778236406299 22.764966632506894 98-99 21.479011190782558 27.436056965963086 27.77264755269276 23.312284290561603 100-101 21.63207950247654 27.889861218805876 27.684142230943298 22.79391704777428 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1059.0 1 714.0 2 968.0 3 1534.5 4 1099.0 5 903.0 6 1849.5 7 3637.0 8 5222.0 9 5120.0 10 3532.0 11 2304.0 12 2066.5 13 2127.0 14 2127.5 15 2032.0 16 2003.0 17 2055.0 18 2022.5 19 1982.5 20 2036.5 21 2206.0 22 2447.5 23 2678.0 24 2912.5 25 3401.0 26 4173.0 27 4850.5 28 5451.0 29 6404.5 30 7823.5 31 9484.5 32 11458.5 33 13432.0 34 15522.5 35 17736.0 36 20693.0 37 23330.5 38 23755.0 39 23935.5 40 23784.0 41 23039.5 42 22367.5 43 22897.0 44 23821.0 45 24431.0 46 24480.5 47 24368.0 48 28675.5 49 29183.0 50 25803.0 51 23745.0 52 21795.5 53 21817.0 54 21770.0 55 20925.0 56 19241.5 57 17766.5 58 17799.0 59 17340.0 60 16312.5 61 15447.0 62 13923.0 63 10409.5 64 7319.0 65 6317.0 66 4602.0 67 3397.0 68 2729.5 69 1951.5 70 1549.5 71 1317.0 72 1178.0 73 1255.5 74 1001.5 75 772.5 76 597.0 77 315.5 78 175.0 79 83.0 80 54.5 81 49.5 82 32.5 83 15.5 84 13.0 85 12.5 86 9.5 87 7.0 88 7.0 89 6.0 90 4.5 91 4.5 92 4.0 93 5.5 94 8.0 95 6.5 96 5.0 97 7.5 98 5.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04297286534496751 2 0.013610174361455986 3 0.011467832100856432 4 0.005796926116916438 5 0.010081610638115544 6 0.004914785186081328 7 0.0037806039892933295 8 0.006931107313704437 9 0.011845892499785765 10-11 0.002520402659528886 12-13 0.006805087180727993 14-15 0.007246157646145548 16-17 0.01638261728693776 18-19 0.011341811967879988 20-21 0.0026464227925053303 22-23 0.008380338842933547 24-25 0.017705828683190428 26-27 0.007561207978586659 28-29 0.01575251662205554 30-31 0.013358134095503097 32-33 0.012602013297644432 34-35 0.26546141011487995 36-37 0.4657704114809382 38-39 0.45965843503158066 40-41 0.48347624016412866 42-43 0.4725754986616662 44-45 0.48984025687943905 46-47 0.49822059572237265 48-49 0.5060968540334004 50-51 0.5173756559347921 52-53 0.504017521839289 54-55 0.4887690857491393 56-57 0.4845474112944284 58-59 0.4812078777705526 60-61 0.47698620331584174 62-63 0.47616707245149487 64-65 0.49273871993789725 66-67 0.4635020490873622 68-69 0.49299076020385013 70-71 0.47320559932654843 72-73 0.4827201193662699 74-75 0.49765350512397855 76-77 0.4832872099646639 78-79 0.4815859381694819 80-81 0.48637470322258686 82-83 0.48505149182633417 84-85 0.4858706226906811 86-87 0.4862486830896104 88-89 0.5030723708419658 90-91 0.5294105786340425 92-93 0.5156743841396101 94-95 0.48574460255770463 96-97 0.45474364984549925 98-99 0.4465523412020304 100-101 0.45512171024442866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 793524.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.58675834669643 #Duplication Level Percentage of deduplicated Percentage of total 1 74.10737665368976 33.04207694565678 2 13.02737916516086 11.616972134556303 3 4.819368312326435 6.4464003097627485 4 2.376794167229016 4.238941886963108 5 1.3450418891424516 2.9985528838689266 6 0.8555288450069661 2.288715472257234 7 0.5975728166686644 1.8650684339952328 8 0.4084498263517148 1.456916296343522 9 0.31295438392052777 1.2558259340463447 >10 1.847396799247178 15.604312609890425 >50 0.19134420770456387 5.94905535907257 >100 0.10372705768758048 8.336203478990956 >500 0.0039568904839599245 1.1122331016189795 >1k 0.0025437153111170945 2.0542161556187386 >5k 5.652700691371322E-4 1.7345089973582182 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8026 1.011437587268942 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5738 0.7231035230188375 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4323 0.5447850348571688 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1812 0.2283484809533171 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1696 0.21373014552804956 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1630 0.20541281675160425 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1540 0.19407100478372424 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1471 0.1853756156083496 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1412 0.17794042776273938 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1263 0.15916342794924918 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1154 0.14542723345481673 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 882 0.11114975728522389 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 859 0.10825129422676566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.4107046541755514E-4 0.0 18-19 0.0 0.0 0.0 6.301006648822216E-4 0.0 20-21 0.0 0.0 0.0 0.001512241595717332 0.0 22-23 0.0 0.0 0.0 0.003024483191434664 0.0 24-25 0.0 0.0 0.0 0.005859936183404661 0.0 26-27 0.0 0.0 0.0 0.00699411738019266 0.0 28-29 0.0 0.0 0.0 0.008695389175374657 0.0 30-31 0.0 0.0 0.0 0.01285405356359732 0.0 32-33 0.0 0.0 0.0 0.019092050145931315 0.0 34-35 0.0 0.0 0.0 0.027787439321305972 0.0 36-37 0.0 0.0 0.0 0.04240577474657351 0.0 38-39 0.0 0.0 0.0 0.06672766041102726 0.0 40-41 0.0 0.0 0.0 0.09621637152751523 0.0 42-43 0.0 0.0 0.0 0.12041223705899254 0.0 44-45 0.0 0.0 0.0 0.14895579717815718 0.0 46-47 0.0 0.0 0.0 0.1839893941456087 0.0 48-49 0.0 0.0 0.0 0.23067985341338132 0.0 50-51 0.0 0.0 0.0 0.2812769368034237 0.0 52-53 0.0 0.0 0.0 0.32746331553929053 0.0 54-55 0.0 0.0 0.0 0.3751619358708747 0.0 56-57 0.0 0.0 0.0 0.42972865344967515 0.0 58-59 0.0 0.0 0.0 0.4987246762542784 0.0 60-61 0.0 0.0 0.0 0.5843553566117723 0.0 62-63 0.0 0.0 0.0 0.6621097786582384 0.0 64-65 0.0 0.0 0.0 0.7372807879786876 0.0 66-67 0.0 0.0 0.0 0.8194459146793291 0.0 68-69 0.0 0.0 0.0 0.9298395511666944 0.0 70-71 0.0 0.0 0.0 1.058191056603203 0.0 72-73 0.0 0.0 0.0 1.185849451308341 0.0 74-75 0.0 0.0 0.0 1.3034892454418516 0.0 76-77 0.0 0.0 0.0 1.4318407508783602 0.0 78-79 0.0 0.0 0.0 1.6003926787343548 0.0 80-81 0.0 0.0 0.0 1.7945897036510552 0.0 82-83 0.0 0.0 0.0 1.982548731985422 0.0 84-85 0.0 0.0 0.0 2.1730281629793176 0.0 86-87 0.0 0.0 0.0 2.3735261945448407 0.0 88-89 0.0 0.0 0.0 2.599354272838629 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 325 0.0 36.43693 1 TACGGGA 60 0.0040105763 31.56872 2 CATATAG 140 1.7578714E-8 30.43935 3 GTACATG 8125 0.0 30.257225 1 GTATCAA 8310 0.0 29.98264 1 GGTATCA 5620 0.0 29.33108 1 TACATGG 8245 0.0 29.290567 2 ATGGGAT 1870 0.0 29.119043 5 ACATGGG 8235 0.0 28.289312 3 ATACCGT 70 0.008523 27.055492 6 TACCGTC 70 0.008523 27.055492 7 TACCTGG 685 0.0 26.960148 2 CATGGGA 5605 0.0 25.765888 4 ATGGGTA 515 0.0 25.74374 5 TAGCCCT 490 0.0 25.124542 4 GTGTACG 115 1.3056968E-4 24.713745 1 ATCAACG 10080 0.0 24.473612 3 TCAACGC 10065 0.0 24.463041 4 CATGGGG 2220 0.0 24.101625 4 CAACGCA 10235 0.0 24.010456 5 >>END_MODULE