FastQCFastQC Report
Thu 26 May 2016
SRR936335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936335_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793524
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81271.0241656206995629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61140.7704870930179806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46750.5891441216648772No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19420.24473109824025485No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16670.21007556167173266No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16490.2078071992781567No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16100.20289241409207534No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15920.20062405169849937No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG14420.1817210317520327No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12830.16168383060877806No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12030.1516022199706625No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT8870.1117798579501061No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8240.10384058957259013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC352.8056122E-454.2142376
TATCGCT500.001626004337.9499667
TACCTGG7050.031.6349432
GTATCAA81000.030.0116921
GTACATG76600.029.0650921
AGACCCG2500.028.4624775
GGTATCA54600.028.4040071
TACATGG77400.028.3243062
GGACAAT2600.027.3677656
GTACACG1056.8320056E-527.1842021
ACATGGG77350.027.107123
AGGCCGG700.00844412227.1071196
TCGCGCA459.0181525E-426.35414518-19
GTACCTG9000.025.9005051
GTGTAAG2651.8189894E-1225.1325661
ATGGGAG17250.025.0249795
ACCTGGG9050.024.6360283
ATCAACG97850.024.5307673
TCAACGC98750.024.3552344
CATGGGA54900.024.0211544
Produced by FastQC (version 0.11.5)