##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936335_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 793524 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46343777881955 34.0 31.0 34.0 31.0 34.0 2 32.56354061124805 34.0 31.0 34.0 31.0 34.0 3 32.642673693549284 34.0 31.0 34.0 31.0 34.0 4 36.13962400633125 37.0 35.0 37.0 35.0 37.0 5 36.12566223579879 37.0 35.0 37.0 35.0 37.0 6 36.186372434860196 37.0 37.0 37.0 35.0 37.0 7 36.14212046516551 37.0 36.0 37.0 35.0 37.0 8 36.12458602386317 37.0 36.0 37.0 35.0 37.0 9 37.860321805011566 39.0 38.0 39.0 35.0 39.0 10-11 37.79986742682011 39.0 38.0 39.0 35.0 39.0 12-13 37.74310228802153 39.0 38.0 39.0 35.0 39.0 14-15 38.97242553470342 40.0 38.0 41.0 35.5 41.0 16-17 38.92241381483106 40.0 38.0 41.0 35.5 41.0 18-19 39.042599845751354 40.0 38.5 41.0 35.5 41.0 20-21 39.035606358471824 40.0 38.5 41.0 35.0 41.0 22-23 38.90316865022356 40.0 38.0 41.0 35.0 41.0 24-25 39.015668083132965 40.0 38.5 41.0 35.0 41.0 26-27 38.890922643801574 40.0 38.0 41.0 35.0 41.0 28-29 38.76237391685696 40.0 38.0 41.0 35.0 41.0 30-31 38.546945650037046 40.0 38.0 41.0 35.0 41.0 32-33 38.272501524843605 40.0 37.0 41.0 34.0 41.0 34-35 38.07380621128031 40.0 37.0 41.0 34.0 41.0 36-37 37.87230568955697 40.0 37.0 41.0 33.0 41.0 38-39 37.69141828098457 40.0 36.0 41.0 33.0 41.0 40-41 37.48969092302186 40.0 36.0 41.0 33.0 41.0 42-43 37.39955691321245 40.0 36.0 41.0 33.0 41.0 44-45 37.125363568083635 40.0 36.0 41.0 32.5 41.0 46-47 36.704213735186336 40.0 35.5 41.0 31.0 41.0 48-49 36.31048336282204 40.0 35.0 41.0 30.5 41.0 50-51 36.05132232925532 39.5 35.0 41.0 30.0 41.0 52-53 35.81688518557725 39.0 35.0 41.0 29.0 41.0 54-55 35.61230599200528 39.0 35.0 41.0 28.0 41.0 56-57 35.38145286090906 39.0 35.0 41.0 28.0 41.0 58-59 35.14585507180627 38.0 35.0 41.0 27.5 41.0 60-61 34.88551385969423 38.0 35.0 40.0 27.0 41.0 62-63 34.59698509433868 37.0 34.5 40.0 26.5 41.0 64-65 34.24834598575468 37.0 34.0 40.0 26.0 41.0 66-67 33.91592314788211 36.0 34.0 39.5 26.0 41.0 68-69 33.59260400441575 36.0 34.0 39.0 25.5 41.0 70-71 33.245577953533854 35.0 34.0 39.0 25.0 40.5 72-73 32.87075375161936 35.0 33.0 37.5 24.0 40.0 74-75 32.46005035764514 35.0 33.0 37.0 23.5 39.5 76-77 31.745525025078006 35.0 32.0 36.0 21.5 39.0 78-79 31.786765113594548 35.0 33.0 36.0 20.5 39.0 80-81 31.62351422263221 35.0 33.0 36.0 20.0 37.5 82-83 31.36627688639537 35.0 33.0 35.5 20.0 37.0 84-85 31.075108629354624 35.0 32.5 35.0 19.0 37.0 86-87 30.67553974422954 35.0 32.0 35.0 15.5 36.0 88-89 30.18084708212984 35.0 31.0 35.0 6.5 36.0 90-91 29.948862920340154 34.0 31.0 35.0 4.5 36.0 92-93 29.670654573774705 34.0 31.0 35.0 2.0 35.0 94-95 29.532341806927075 34.0 31.0 35.0 2.0 35.0 96-97 29.310437365473508 34.0 31.0 35.0 2.0 35.0 98-99 28.9024358431503 34.0 30.5 35.0 2.0 35.0 100-101 25.866435671762922 31.5 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1746352693658153 1101 2 0.13994774794268494 1101 3 0.20730044650648694 1101 4 0.025224916025592847 1101 5 0.045224073908023854 1101 6 0.16836177166408817 1101 7 0.12695360072589779 1101 8 0.06493150941539483 1101 9 0.10909690600386313 1101 10-11 0.038873626480189216 1101 12-13 0.24861227882750825 1101 14-15 0.37356889881422006 1101 16-17 0.32948780670774624 1101 18-19 0.25963685206123444 1101 20-21 0.0740305294293293 1101 22-23 0.1638701812989396 1101 24-25 -0.025813114965814066 1101 26-27 -0.09416676269297852 1101 28-29 0.09969919257586213 1101 30-31 0.07957772529408658 1101 32-33 0.28767478578805594 1101 34-35 0.0816234082444467 1101 36-37 0.11303241094307026 1101 38-39 0.16234619573696563 1101 40-41 0.33779675488999317 1101 42-43 0.024832103169423192 1101 44-45 -0.008411721297079566 1101 46-47 0.21580616428930455 1101 48-49 0.46061028844896157 1101 50-51 0.45258261072178385 1101 52-53 0.629354067699694 1101 54-55 0.4515806869546779 1101 56-57 0.5087848782517881 1101 58-59 0.2606647759037699 1101 60-61 0.38617252411476954 1101 62-63 0.33508549340262306 1101 64-65 0.5567375622939679 1101 66-67 0.46092468061215186 1101 68-69 0.63310058233143 1101 70-71 0.617259947891462 1101 72-73 0.5730753540980942 1101 74-75 0.3881202810053779 1101 76-77 0.2879214521045306 1101 78-79 0.25399778948316154 1101 80-81 0.30961687946115646 1101 82-83 0.30497046914196346 1101 84-85 0.3715558795861611 1101 86-87 0.3158730979159863 1101 88-89 0.39319734717161126 1101 90-91 0.4031021321805852 1101 92-93 0.3577804593152436 1101 94-95 0.3612096344219431 1101 96-97 0.20445770688874632 1101 98-99 0.02536539544809102 1101 100-101 0.44352150975763394 1102 1 0.13182091965457232 1102 2 0.21613561113827018 1102 3 0.13638397924131596 1102 4 0.22227257448231086 1102 5 0.144098729827995 1102 6 0.024401388093572507 1102 7 0.13200010775142346 1102 8 0.1319349097356337 1102 9 0.08637862883278302 1102 10-11 0.14161862045316553 1102 12-13 0.13599466193025478 1102 14-15 0.11968731631597507 1102 16-17 0.20999308709922815 1102 18-19 0.3155284915913086 1102 20-21 0.22080427319991003 1102 22-23 0.3319746376974777 1102 24-25 0.15322319109899496 1102 26-27 0.3346064233531152 1102 28-29 0.13071362144799537 1102 30-31 0.1324423601644611 1102 32-33 0.1857056585369179 1102 34-35 0.08647875970083163 1102 36-37 0.10088841007949867 1102 38-39 0.06163194857506227 1102 40-41 0.09052240841715076 1102 42-43 0.03070620136491442 1102 44-45 0.014655133676569676 1102 46-47 0.07238101631240568 1102 48-49 0.06408842025184924 1102 50-51 0.05416492905651893 1102 52-53 -0.22621103314050117 1102 54-55 -0.15561485844589384 1102 56-57 0.23632747221036965 1102 58-59 0.12916323766366133 1102 60-61 0.05532946058815469 1102 62-63 0.12665395858082462 1102 64-65 0.059141624687143235 1102 66-67 0.07696285775264045 1102 68-69 0.0159132228870007 1102 70-71 -0.051782295240428766 1102 72-73 -0.15462915987200887 1102 74-75 0.03450496697050909 1102 76-77 -0.0049761065015232475 1102 78-79 0.09752233835601842 1102 80-81 0.08451657900958409 1102 82-83 0.007593123550687864 1102 84-85 0.11056555880462682 1102 86-87 -0.06427460911674032 1102 88-89 0.06969240861126025 1102 90-91 0.16581959975160387 1102 92-93 0.09907051202920414 1102 94-95 -0.045252323370906566 1102 96-97 -0.2340464220715468 1102 98-99 -0.04452782729987348 1102 100-101 -0.1734409607819174 1103 1 0.0842949689028103 1103 2 0.15275991873031813 1103 3 0.1206738828860594 1103 4 0.04590055940062143 1103 5 0.07609146827235236 1103 6 0.13765372715236168 1103 7 0.261590947019684 1103 8 0.2314321866522704 1103 9 0.10432305000301056 1103 10-11 0.16811631630569224 1103 12-13 0.26650483276300463 1103 14-15 0.2399451946383735 1103 16-17 0.1948648461645348 1103 18-19 0.10325599706074229 1103 20-21 0.2880318484920039 1103 22-23 0.37036545834636314 1103 24-25 0.2372082025033535 1103 26-27 0.10457570138690642 1103 28-29 0.37142720893578485 1103 30-31 0.2862732962534622 1103 32-33 0.3903558470580748 1103 34-35 0.417182106533204 1103 36-37 0.4231781275305053 1103 38-39 0.5173643881784642 1103 40-41 0.5471625424766415 1103 42-43 0.5633482904586202 1103 44-45 0.738774204569836 1103 46-47 0.7082326199059494 1103 48-49 0.6266495150710085 1103 50-51 0.5862018885128393 1103 52-53 0.5477614101867871 1103 54-55 0.5605560674024517 1103 56-57 0.4384407710978664 1103 58-59 0.5373580894069008 1103 60-61 0.5597399246760304 1103 62-63 0.6748083131867944 1103 64-65 0.62444907709137 1103 66-67 0.6054970475771526 1103 68-69 0.27632843491511494 1103 70-71 0.14319745619093993 1103 72-73 0.49738638730483586 1103 74-75 0.46506634108027356 1103 76-77 0.46711810752817584 1103 78-79 0.49217159590850557 1103 80-81 0.42350554703081045 1103 82-83 0.3821101835516174 1103 84-85 0.2805837126937476 1103 86-87 0.2822901164802438 1103 88-89 0.5360378910857726 1103 90-91 0.5533236727395483 1103 92-93 0.44498355955515123 1103 94-95 0.303081242704593 1103 96-97 0.3297365691307981 1103 98-99 0.26358789210616607 1103 100-101 0.5359455308626373 1104 1 -0.02528825832747117 1104 2 0.056545675457250866 1104 3 -0.010469483551425185 1104 4 0.03418678298096012 1104 5 0.01650399692017146 1104 6 0.040760424376706794 1104 7 0.1469996559578064 1104 8 0.07889844588323314 1104 9 0.12736863187212322 1104 10-11 0.09967985081041064 1104 12-13 0.21196786646132892 1104 14-15 0.34261050318603026 1104 16-17 0.32305854788765487 1104 18-19 0.28206085233618694 1104 20-21 0.26152730728157536 1104 22-23 0.03656527160499223 1104 24-25 0.06822848940599613 1104 26-27 0.24851509532630445 1104 28-29 0.26008267112065653 1104 30-31 0.32646513056294424 1104 32-33 0.2655150016289909 1104 34-35 0.548304252361234 1104 36-37 0.47001201259968184 1104 38-39 0.37081485927599545 1104 40-41 0.40844341846339915 1104 42-43 0.453467974695954 1104 44-45 0.36709522994968324 1104 46-47 0.29477026526711825 1104 48-49 0.17267701698086313 1104 50-51 0.18765762232151673 1104 52-53 0.3589192066387028 1104 54-55 0.12237170274750042 1104 56-57 0.14937362908954555 1104 58-59 0.0479471735254009 1104 60-61 0.038116123640463684 1104 62-63 0.024332971534981596 1104 64-65 0.021614003668062765 1104 66-67 -0.12410994673572162 1104 68-69 0.004195339252603958 1104 70-71 0.15083687559506842 1104 72-73 0.11620057964844221 1104 74-75 0.14828508312254485 1104 76-77 0.16775896993374673 1104 78-79 0.33208331817316505 1104 80-81 0.2527008615202391 1104 82-83 0.2535557314677561 1104 84-85 0.22296975802096952 1104 86-87 -0.020549201064955724 1104 88-89 0.2263867579052281 1104 90-91 0.3791873515444024 1104 92-93 0.4485698425532014 1104 94-95 0.38862151942134204 1104 96-97 0.3741725253314634 1104 98-99 -0.05849851821553642 1104 100-101 -0.16416481630653124 1105 1 0.11076444601606994 1105 2 0.14634382647731314 1105 3 0.1296105828098888 1105 4 0.034102629147753305 1105 5 0.05563552936147431 1105 6 0.0059628143455725535 1105 7 0.12207809935232916 1105 8 0.15094614908468884 1105 9 0.14456606878680134 1105 10-11 0.19320781993797453 1105 12-13 0.19315948473954592 1105 14-15 0.21544203923392757 1105 16-17 0.19630784760753528 1105 18-19 0.2824575775498772 1105 20-21 0.28693875941608127 1105 22-23 0.16963652585776856 1105 24-25 0.11309149898751514 1105 26-27 0.24662463957318437 1105 28-29 0.20833708018041364 1105 30-31 0.25232560350748656 1105 32-33 0.23824615417585449 1105 34-35 0.13069987155311225 1105 36-37 0.30655821906510283 1105 38-39 0.3443064018157642 1105 40-41 0.27164835709120894 1105 42-43 0.41223259642446664 1105 44-45 0.26232671310087596 1105 46-47 0.3285219633712515 1105 48-49 0.3509959840526733 1105 50-51 0.40602179202372923 1105 52-53 0.5158450671116412 1105 54-55 0.40794473024175915 1105 56-57 0.36613587046678475 1105 58-59 0.3954454002839114 1105 60-61 0.5457651059801805 1105 62-63 0.38043292224487857 1105 64-65 0.3240508582382162 1105 66-67 0.4226074526703911 1105 68-69 0.48670937490978616 1105 70-71 0.2411760155428837 1105 72-73 0.5311420927343562 1105 74-75 0.4576345739918537 1105 76-77 0.5573521462600688 1105 78-79 0.49306960109835885 1105 80-81 0.419947588537223 1105 82-83 0.33100130197063393 1105 84-85 0.31956333107250856 1105 86-87 0.33114469537774127 1105 88-89 0.6163195550584284 1105 90-91 0.5108820895254311 1105 92-93 0.29632654179942364 1105 94-95 0.42226201424588083 1105 96-97 0.4608329405705156 1105 98-99 0.26492870675170366 1105 100-101 0.4449466394345656 1106 1 -0.0035319830018067933 1106 2 -0.09679527257244303 1106 3 -0.07097912430092634 1106 4 -0.0828347829269589 1106 5 -0.07794543333736925 1106 6 -0.011549820109436837 1106 7 0.05943064777200391 1106 8 0.13220132043111477 1106 9 0.0011414419670359166 1106 10-11 0.06087889353429432 1106 12-13 0.09347775196893338 1106 14-15 0.05812000795881289 1106 16-17 -0.17216187037674047 1106 18-19 0.05016615270876912 1106 20-21 0.1020878033246504 1106 22-23 0.052828087410929925 1106 24-25 0.12458404133550971 1106 26-27 -0.04031985613000444 1106 28-29 0.1707799597448414 1106 30-31 0.17752982828669417 1106 32-33 0.07942406039813221 1106 34-35 0.15966238784843512 1106 36-37 -0.013595993709635934 1106 38-39 0.2901126754898655 1106 40-41 0.12313026407542793 1106 42-43 0.16930382359861795 1106 44-45 0.27505938648607753 1106 46-47 0.3453808007434489 1106 48-49 0.2544602156972431 1106 50-51 0.31332050883533924 1106 52-53 0.18214267078164426 1106 54-55 0.22585678610122528 1106 56-57 0.3616604855797121 1106 58-59 0.08670733778527051 1106 60-61 0.22721215040068898 1106 62-63 0.2010415190316479 1106 64-65 0.1738263449152413 1106 66-67 0.35110149706509475 1106 68-69 0.22121418562610273 1106 70-71 0.25476388329134636 1106 72-73 0.018807253385894285 1106 74-75 0.21137828156696514 1106 76-77 0.08641089672387636 1106 78-79 0.038536773341640185 1106 80-81 0.15636815367982138 1106 82-83 0.26229903461425863 1106 84-85 0.4673403217804548 1106 86-87 0.35214259409971405 1106 88-89 0.34631822563795467 1106 90-91 0.29658149366727216 1106 92-93 0.2035329928618701 1106 94-95 0.3494534249991048 1106 96-97 0.4408827045487804 1106 98-99 0.2569402786624373 1106 100-101 0.6274780544339968 1107 1 -0.14874873782510178 1107 2 0.03686411862398842 1107 3 -0.0698269391937103 1107 4 0.04578971718451186 1107 5 0.10715192038543364 1107 6 0.08726978203157643 1107 7 0.1546220505771032 1107 8 0.04826542453813687 1107 9 0.2013592523023675 1107 10-11 0.1973694245061779 1107 12-13 0.09446026598159563 1107 14-15 0.13935793615637948 1107 16-17 0.12921443840886582 1107 18-19 0.21198502684241305 1107 20-21 0.19731570514427688 1107 22-23 0.13136706145322563 1107 24-25 0.14627037472342863 1107 26-27 0.20294508922997778 1107 28-29 0.2282313815294259 1107 30-31 0.2247999979380566 1107 32-33 0.2769734968336195 1107 34-35 0.26438200636468423 1107 36-37 0.16519492217887688 1107 38-39 0.48401319693253697 1107 40-41 0.3117798597255401 1107 42-43 0.03743584349354734 1107 44-45 0.04072584862673523 1107 46-47 -0.13652259327032112 1107 48-49 -0.05870330186075989 1107 50-51 -0.04240381904023849 1107 52-53 0.06599392841930296 1107 54-55 0.10779332516602835 1107 56-57 -0.2837999248270435 1107 58-59 0.015553988655426565 1107 60-61 0.010028217069589118 1107 62-63 -0.15489391125226604 1107 64-65 -0.05544270806357332 1107 66-67 -0.0046021151535882154 1107 68-69 -0.061375981736674134 1107 70-71 0.21642413468077137 1107 72-73 0.1035865724523859 1107 74-75 0.08480364502810289 1107 76-77 -0.030336626768662 1107 78-79 0.10532405116622456 1107 80-81 0.2924315475357524 1107 82-83 0.5795376178211598 1107 84-85 0.567797447275904 1107 86-87 0.5581302081098123 1107 88-89 0.6446601839185924 1107 90-91 0.2772054492529037 1107 92-93 0.5106278094099288 1107 94-95 0.6452039532483589 1107 96-97 0.16291903389221218 1107 98-99 0.037265534204863116 1107 100-101 0.2597287809616766 1108 1 -0.08420392985924252 1108 2 -0.04471482874443211 1108 3 -0.05969180405857344 1108 4 0.02626624634525143 1108 5 -0.1166745376515479 1108 6 -0.053021825365291875 1108 7 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-0.9856877371624755 2316 82-83 -0.9510963996549648 2316 84-85 -1.1213409392309046 2316 86-87 -1.0990278898377603 2316 88-89 -1.0152666602187743 2316 90-91 -1.1427295233136476 2316 92-93 -1.1835654439938494 2316 94-95 -1.2533176886943345 2316 96-97 -1.231030366636137 2316 98-99 -1.563958810440532 2316 100-101 -1.6104703655532582 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 17.0 5 38.0 6 48.0 7 125.0 8 217.0 9 355.0 10 615.0 11 1094.0 12 1910.0 13 2792.0 14 3446.0 15 4722.0 16 6632.0 17 9691.0 18 8940.0 19 4322.0 20 3232.0 21 3104.0 22 3317.0 23 3712.0 24 4176.0 25 4995.0 26 5980.0 27 7389.0 28 9208.0 29 11335.0 30 14096.0 31 17429.0 32 21828.0 33 28178.0 34 40212.0 35 67508.0 36 103364.0 37 167771.0 38 192137.0 39 39536.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.785029700268005 18.85692191249256 13.072161353134353 21.28588703410508 2 16.172053527876265 20.70720120597796 39.879200535430016 23.24154473071576 3 19.865057641608825 27.632434557744944 28.660380782433805 23.84212701821243 4 11.928939767417242 15.932095311546973 36.99648655869262 35.14247836234317 5 12.705349806685115 39.8330737318594 32.335127860026915 15.126448601428564 6 30.896356001834853 35.82563350320847 17.721203139413554 15.55680735554312 7 29.142911871600607 34.331034726107845 19.465951880472424 17.060101521819128 8 28.29895504105736 33.20227239503783 19.274905358880133 19.223867205024675 9 29.503959552578117 15.193239271906082 19.999143063095758 35.30365811242004 10-11 25.8425585728409 27.108184350624843 25.425934175613612 21.623322900920645 12-13 27.17749024129751 24.63313894691077 27.0856844008834 21.103686410908324 14-15 23.909610799421316 25.291925638039935 26.662709634491204 24.135753928047546 16-17 21.66949582934793 29.00742387410037 27.185102851720373 22.13797744483133 18-19 21.36614645555774 26.18364409898125 31.583669807088384 20.86653963837263 20-21 22.62801456290492 25.28672766888925 31.33349631957738 20.751761448628457 22-23 21.549442738971976 25.63262106754175 31.2406430051265 21.57729318835977 24-25 21.263265478439383 25.82025111309667 31.80816300297288 21.108320405491064 26-27 21.327757218462175 26.188164261405273 31.593496437003743 20.89058208312881 28-29 20.938391809014796 25.709871311703182 32.265079114033725 21.0866577652483 30-31 21.16228353447412 26.099301147866676 31.927227634494855 20.81118768316435 32-33 20.54833449273609 26.128275312786702 31.775448890501973 21.54794130397524 34-35 21.18073977550406 26.151181269068736 31.75267891686442 20.915400038562794 36-37 21.609189897967735 25.82696038617634 31.13656999446141 21.427279721394516 38-39 21.405617732567297 26.03541422239809 31.332866218118443 21.226101826916167 40-41 21.25797006111404 26.156418927646747 31.603249585862425 20.982361425376787 42-43 21.071727655327162 26.36426591488662 31.094733485865373 21.46927294392085 44-45 20.95073661923906 26.653581015513744 30.63693304263444 21.75874932261276 46-47 21.332867831806904 26.942707391328184 29.827346408128463 21.897078368736448 48-49 21.64522461377522 27.05542794304386 29.23460851317245 22.064738930008478 50-51 22.02464483646251 27.033766135562352 29.010835284146587 21.93075374382855 52-53 21.919458680464317 27.347777511016506 28.94065737959352 21.79210642892566 54-55 21.85435429285785 27.17865867528479 28.95441396357881 22.012573068278556 56-57 21.80973366847153 27.718592293543757 28.186580147409558 22.28509389057515 58-59 21.689729764470965 26.989589942930888 28.833671597702114 22.48700869489604 60-61 22.070932764721896 27.18893980179254 28.972214660899898 21.76791277258567 62-63 22.302728083838673 27.128417035905656 28.819859260715493 21.748995619540178 64-65 21.963689475896217 27.055076989577408 28.943002445443796 22.038231089082583 66-67 21.975201758232895 27.564950776536058 28.486157948593867 21.97368951663718 68-69 22.2373236348239 27.38171750318831 28.695099329068814 21.68585953291898 70-71 21.87019153824149 27.292025561955985 28.85858380916495 21.979199090637575 72-73 22.091243564224175 27.49386116304259 28.32491192651417 22.089983346219064 74-75 21.75107495173429 27.88069421970854 28.233109521577166 22.13512130698 76-77 21.744644772486087 27.78922900253975 28.644146228597872 21.821979996376292 78-79 21.949184186823363 27.440312045070936 28.58733250410619 22.023171263999515 80-81 21.401386743019028 28.399561948497443 28.22369863669702 21.975352671786506 82-83 22.03354907980099 28.142564056033592 27.68782040618809 22.13606645797733 84-85 21.66185475547468 27.63942045350005 27.83821896695345 22.860505824071822 86-87 21.574371061643404 27.679028826814516 28.0864602549317 22.66013985661038 88-89 21.9603038222451 27.557232892567534 28.06070866925324 22.421754615934127 90-91 21.595834315696724 27.84075789361905 28.219755269839688 22.34365252084454 92-93 21.566137900524556 27.746192251785374 28.190987802565886 22.496682045124185 94-95 21.295207009429713 27.80552046149823 28.329778419097607 22.56949410997445 96-97 21.599080536253034 27.816361817049902 27.849946944320518 22.734610702376543 98-99 21.4119722747322 27.448141146817896 27.898298676748585 23.241587901701326 100-101 21.52825385623551 27.94397116644823 27.829796350438553 22.69797862687771 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1179.0 1 812.0 2 1040.5 3 1598.0 4 1148.5 5 881.5 6 1854.5 7 3697.5 8 5057.0 9 4960.5 10 3518.0 11 2197.5 12 1874.5 13 1899.5 14 1911.5 15 1839.0 16 1762.0 17 1755.0 18 1812.5 19 1872.5 20 1919.0 21 2054.0 22 2295.0 23 2627.0 24 2940.5 25 3388.5 26 4184.0 27 4958.5 28 5505.5 29 6405.5 30 7832.5 31 9484.0 32 11703.0 33 13791.5 34 15744.0 35 17854.5 36 20830.0 37 23633.0 38 24127.5 39 24119.5 40 23899.0 41 23256.0 42 22520.5 43 23150.5 44 24314.5 45 24855.0 46 24607.0 47 24354.0 48 29053.5 49 29263.0 50 25794.0 51 24020.5 52 21566.5 53 21435.5 54 21446.5 55 20848.0 56 19411.0 57 17738.0 58 17717.5 59 17497.5 60 16408.5 61 15363.0 62 13888.5 63 10396.5 64 7269.5 65 6174.0 66 4358.0 67 3227.0 68 2648.0 69 1880.5 70 1434.0 71 1182.0 72 1066.0 73 1209.0 74 972.5 75 730.5 76 548.5 77 261.0 78 135.5 79 54.0 80 33.5 81 29.0 82 15.5 83 6.0 84 3.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2667846215111326 2 0.018020879015631538 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.410704654175551E-4 12-13 1.8903019946466646E-4 14-15 0.0 16-17 3.780603989293329E-4 18-19 0.0 20-21 1.260201329764443E-4 22-23 0.0 24-25 0.0021423422605995534 26-27 0.002583412726017108 28-29 0.0022053523270877755 30-31 0.0010711711302997767 32-33 0.0015122415957173317 34-35 0.0016382617286937761 36-37 6.301006648822215E-5 38-39 1.260201329764443E-4 40-41 9.451509973233324E-4 42-43 0.0037175939228051074 44-45 0.004284684521199107 46-47 0.014429305225802875 48-49 0.013421144161991319 50-51 0.006490036848286883 52-53 0.00699411738019266 54-55 3.1505033244111076E-4 56-57 0.002583412726017108 58-59 0.001575251662205554 60-61 0.0015122415957173317 62-63 0.0 64-65 9.451509973233324E-4 66-67 0.0 68-69 0.0 70-71 1.260201329764443E-4 72-73 0.0013232113962526651 74-75 0.0 76-77 0.19123555179175425 78-79 0.10308446877473146 80-81 0.001260201329764443 82-83 0.0 84-85 8.821409308351102E-4 86-87 0.12576809271049144 88-89 0.5932397759866116 90-91 0.3137271210448581 92-93 0.2991717956860788 94-95 0.002709432858993553 96-97 0.0022683623935759976 98-99 0.0030244831914346634 100-101 5.040805319057772E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 793524.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.78183413218971 #Duplication Level Percentage of deduplicated Percentage of total 1 72.33991792262492 30.948343697019563 2 13.824685666493352 11.828908182271583 3 5.213950124341675 6.691870481792864 4 2.5544147138232174 4.371301863664389 5 1.4388668059837224 3.07786805159546 6 0.9137935175833605 2.345625762019291 7 0.6303504721550072 1.887728454141809 8 0.4383772295276457 1.5003665536784476 9 0.33369551438812495 1.2848495532487636 >10 1.9844079604454203 16.147284301808963 >50 0.20434511210483106 6.0414652155339095 >100 0.1158444258909629 8.787531618523259 >500 0.004116299397120773 1.14919680599196 >1k 0.00264619246957764 2.1463162325570067 >5k 5.880427710172533E-4 1.7913432261527478 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8127 1.0241656206995629 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6114 0.7704870930179806 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4675 0.5891441216648772 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1942 0.24473109824025485 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1667 0.21007556167173266 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1649 0.2078071992781567 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1610 0.20289241409207534 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1592 0.20062405169849937 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1442 0.1817210317520327 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1283 0.16168383060877806 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1203 0.1516022199706625 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 887 0.1117798579501061 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 824 0.10384058957259013 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.2602013297644433E-4 0.0 14-15 0.0 0.0 0.0 1.2602013297644433E-4 0.0 16-17 0.0 0.0 0.0 4.4107046541755514E-4 0.0 18-19 0.0 0.0 0.0 5.670905983939994E-4 0.0 20-21 0.0 0.0 0.0 0.0013862214627408875 0.0 22-23 0.0 0.0 0.0 0.002898463058458219 0.0 24-25 0.0 0.0 0.0 0.005607895917451772 0.0 26-27 0.0 0.0 0.0 0.006742077114239771 0.0 28-29 0.0 0.0 0.0 0.008317328776445324 0.0 30-31 0.0 0.0 0.0 0.012665023364132654 0.0 32-33 0.0 0.0 0.0 0.018966030012954868 0.0 34-35 0.0 0.0 0.0 0.02791345945428242 0.0 36-37 0.0 0.0 0.0 0.04297286534496751 0.0 38-39 0.0 0.0 0.0 0.06735776107590949 0.0 40-41 0.0 0.0 0.0 0.09741356279079146 0.0 42-43 0.0 0.0 0.0 0.12211350885417455 0.0 44-45 0.0 0.0 0.0 0.1510351293722685 0.0 46-47 0.0 0.0 0.0 0.1868248471375787 0.0 48-49 0.0 0.0 0.0 0.2340193869372571 0.0 50-51 0.0 0.0 0.0 0.2851835609256935 0.0 52-53 0.0 0.0 0.0 0.3314959597945368 0.0 54-55 0.0 0.0 0.0 0.3796356505915385 0.0 56-57 0.0 0.0 0.0 0.4343283883033153 0.0 58-59 0.0 0.0 0.0 0.5033244111079186 0.0 60-61 0.0 0.0 0.0 0.5892071317313654 0.0 62-63 0.0 0.0 0.0 0.6675286443762256 0.0 64-65 0.0 0.0 0.0 0.7433297543615569 0.0 66-67 0.0 0.0 0.0 0.8254318709957102 0.0 68-69 0.0 0.0 0.0 0.9367706584803988 0.0 70-71 0.0 0.0 0.0 1.0662563451136953 0.0 72-73 0.0 0.0 0.0 1.194607850550204 0.0 74-75 0.0 0.0 0.0 1.3135708560799673 0.0 76-77 0.0 0.0 0.0 1.4423634319818934 0.0 78-79 0.0 0.0 0.0 1.6106633195719349 0.0 80-81 0.0 0.0 0.0 1.8054904451535179 0.0 82-83 0.0 0.0 0.0 1.994457634551696 0.0 84-85 0.0 0.0 0.0 2.1875204782716087 0.0 86-87 0.0 0.0 0.0 2.389593761499337 0.0 88-89 0.0 0.0 0.0 2.6163669907904485 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCGC 35 2.8056122E-4 54.214237 6 TATCGCT 50 0.0016260043 37.949966 7 TACCTGG 705 0.0 31.634943 2 GTATCAA 8100 0.0 30.011692 1 GTACATG 7660 0.0 29.065092 1 AGACCCG 250 0.0 28.462477 5 GGTATCA 5460 0.0 28.404007 1 TACATGG 7740 0.0 28.324306 2 GGACAAT 260 0.0 27.367765 6 GTACACG 105 6.8320056E-5 27.184202 1 ACATGGG 7735 0.0 27.10712 3 AGGCCGG 70 0.008444122 27.107119 6 TCGCGCA 45 9.0181525E-4 26.354145 18-19 GTACCTG 900 0.0 25.900505 1 GTGTAAG 265 1.8189894E-12 25.132566 1 ATGGGAG 1725 0.0 25.024979 5 ACCTGGG 905 0.0 24.636028 3 ATCAACG 9785 0.0 24.530767 3 TCAACGC 9875 0.0 24.355234 4 CATGGGA 5490 0.0 24.021154 4 >>END_MODULE