##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936334_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 657213 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43997151608383 34.0 31.0 34.0 31.0 34.0 2 32.56550920325678 34.0 31.0 34.0 31.0 34.0 3 32.65236080235783 34.0 31.0 34.0 31.0 34.0 4 36.153788802108295 37.0 35.0 37.0 35.0 37.0 5 36.13819568389548 37.0 35.0 37.0 35.0 37.0 6 36.18319631534982 37.0 37.0 37.0 35.0 37.0 7 36.13178071644961 37.0 36.0 37.0 35.0 37.0 8 36.11564896007839 37.0 36.0 37.0 35.0 37.0 9 37.87506790036107 39.0 38.0 39.0 35.0 39.0 10-11 37.81870033002999 39.0 38.0 39.0 35.0 39.0 12-13 37.785709503616026 39.0 38.0 39.0 35.0 39.0 14-15 39.05269600570895 40.0 38.0 41.0 36.0 41.0 16-17 39.00345550072807 40.0 38.0 41.0 35.5 41.0 18-19 39.12153974434468 40.0 38.5 41.0 36.0 41.0 20-21 39.10310356003305 40.0 39.0 41.0 35.5 41.0 22-23 38.97826047263216 40.0 38.5 41.0 35.0 41.0 24-25 39.09398094681633 40.5 39.0 41.0 35.0 41.0 26-27 38.961296413795836 40.0 38.0 41.0 35.0 41.0 28-29 38.85310394042723 40.0 38.0 41.0 35.0 41.0 30-31 38.70857925817049 40.0 38.0 41.0 35.0 41.0 32-33 38.491963031772045 40.0 38.0 41.0 34.5 41.0 34-35 38.35310241885051 40.0 38.0 41.0 34.0 41.0 36-37 38.19366248080911 40.0 38.0 41.0 34.0 41.0 38-39 38.03880172790252 40.0 37.0 41.0 34.0 41.0 40-41 37.929280918058524 40.0 37.0 41.0 33.0 41.0 42-43 37.912257517730175 40.0 38.0 41.0 33.5 41.0 44-45 37.742709745546726 40.0 38.0 41.0 33.0 41.0 46-47 37.48334862517935 40.0 37.5 41.0 33.0 41.0 48-49 37.244605630138174 40.0 37.0 41.0 33.0 41.0 50-51 37.07403459761143 40.0 37.0 41.0 32.0 41.0 52-53 36.90673799818324 40.0 37.0 41.0 31.0 41.0 54-55 36.783768732511376 40.0 37.0 41.0 31.0 41.0 56-57 36.596718263333194 40.0 36.0 41.0 31.0 41.0 58-59 36.40811122117182 40.0 36.0 41.0 31.0 41.0 60-61 36.219587104941624 39.0 35.5 41.0 30.0 41.0 62-63 35.94330148673261 39.0 35.0 41.0 29.5 41.0 64-65 35.62565941331045 39.0 35.0 40.5 29.0 41.0 66-67 35.31600866081468 38.0 35.0 40.0 28.0 41.0 68-69 34.967982221897614 37.5 35.0 40.0 28.0 41.0 70-71 34.60412225564619 37.0 35.0 39.5 28.0 41.0 72-73 34.17213369181681 36.5 34.0 39.0 27.5 41.0 74-75 33.69784681678543 36.0 34.0 39.0 26.0 40.0 76-77 32.887475597713376 35.0 33.0 37.0 26.0 39.0 78-79 32.87856524444891 35.0 34.0 37.0 26.0 39.0 80-81 32.673520608995865 35.0 34.0 37.0 26.0 39.0 82-83 32.33485643162871 35.0 33.0 36.0 26.0 38.5 84-85 31.970724863933 35.0 33.0 36.0 25.0 37.0 86-87 31.5570051109762 35.0 33.0 35.5 23.5 37.0 88-89 31.03028241985475 35.0 32.0 35.0 19.5 36.5 90-91 30.78893981098974 35.0 32.0 35.0 18.5 36.0 92-93 30.508571041656204 35.0 32.0 35.0 14.5 36.0 94-95 30.374338304324475 34.5 32.0 35.0 10.5 36.0 96-97 30.164681769837173 34.0 32.0 35.0 2.0 35.0 98-99 29.82822083555864 34.0 31.5 35.0 2.0 35.0 100-101 26.78119955022192 31.5 25.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26664396336607865 1101 2 0.14963325906946778 1101 3 0.31262444146130974 1101 4 0.29182965110264547 1101 5 0.12278076630548895 1101 6 0.15316319147549962 1101 7 0.15922133053894072 1101 8 0.21194388544081733 1101 9 0.34449688767095665 1101 10-11 0.1373372285946317 1101 12-13 0.1581608519067501 1101 14-15 0.32242283018252493 1101 16-17 0.3509097477085632 1101 18-19 0.4092950553347521 1101 20-21 0.3984788418204701 1101 22-23 0.3858362167324856 1101 24-25 0.2029574817919837 1101 26-27 0.3218915628021435 1101 28-29 0.15317927882959737 1101 30-31 0.35554038605353355 1101 32-33 0.4638774994375652 1101 34-35 0.3121853372288328 1101 36-37 0.20524242919801594 1101 38-39 0.4304450803744402 1101 40-41 0.5250096610085322 1101 42-43 0.42977556741408307 1101 44-45 0.2796253629429515 1101 46-47 0.24007706287583375 1101 48-49 0.07695969490853116 1101 50-51 0.057771328495945795 1101 52-53 -0.15068872233107555 1101 54-55 -0.007361020520072259 1101 56-57 -0.015609831635515548 1101 58-59 -0.10767785308277666 1101 60-61 0.018465198180557252 1101 62-63 -0.1137150044174362 1101 64-65 0.17164578519417972 1101 66-67 0.15878685650004343 1101 68-69 0.06758348192826702 1101 70-71 -0.044260788968166764 1101 72-73 0.13500427017724093 1101 74-75 0.01695262654217089 1101 76-77 -0.044378664814097135 1101 78-79 -0.35032227497994484 1101 80-81 -0.2542834421544029 1101 82-83 -0.48427656936012653 1101 84-85 -0.2025936508684154 1101 86-87 -0.22636109715940478 1101 88-89 -0.332341087300577 1101 90-91 -0.06350620400940699 1101 92-93 0.06794734511734646 1101 94-95 0.0872830497872421 1101 96-97 0.2110987070963155 1101 98-99 0.08964775968543748 1101 100-101 0.40614342535794634 1102 1 0.03350556180662778 1102 2 0.04424478789152175 1102 3 0.03866175697231 1102 4 0.016544215290899444 1102 5 0.06310379610220451 1102 6 0.008927602503071341 1102 7 0.019385630176927293 1102 8 0.1643666089161755 1102 9 0.013098948378285513 1102 10-11 0.09502310996472119 1102 12-13 0.16804667758204772 1102 14-15 0.1979728162588259 1102 16-17 0.2581918418327689 1102 18-19 0.1370797949616005 1102 20-21 0.1877227850117933 1102 22-23 0.15842741695526996 1102 24-25 0.12208864302841249 1102 26-27 0.14380050181635085 1102 28-29 0.13567719027487612 1102 30-31 0.12692022453863672 1102 32-33 0.19542859940971624 1102 34-35 0.002807726535429822 1102 36-37 -0.09368544604564732 1102 38-39 4.268549835728663E-5 1102 40-41 0.04538003137890456 1102 42-43 0.14258536969757074 1102 44-45 0.0826328817399471 1102 46-47 0.371461078470368 1102 48-49 0.519197233198696 1102 50-51 0.4901369648089542 1102 52-53 0.18108515681122128 1102 54-55 0.07016613069129818 1102 56-57 0.38876220957862984 1102 58-59 0.2710467361681168 1102 60-61 0.08511794114908611 1102 62-63 0.3609020938838654 1102 64-65 0.3576106974748825 1102 66-67 0.27550921795924666 1102 68-69 0.21604491328444198 1102 70-71 0.47672222412010257 1102 72-73 0.1765804328060412 1102 74-75 0.3486986582881926 1102 76-77 0.4429089987893562 1102 78-79 0.43155603189836 1102 80-81 0.4438716678428136 1102 82-83 0.36518736826170795 1102 84-85 0.3249474240410777 1102 86-87 0.3522938736008321 1102 88-89 0.5159811070742464 1102 90-91 0.38512927357343685 1102 92-93 0.3316343403832889 1102 94-95 0.36002044884043016 1102 96-97 0.18584808052990098 1102 98-99 0.5236494305292112 1102 100-101 0.8188418380563611 1103 1 0.020905060949893084 1103 2 0.09596483534110689 1103 3 0.06925355873848815 1103 4 0.07171753807988068 1103 5 0.1524144590783436 1103 6 0.057385490996544775 1103 7 0.16559625361174568 1103 8 0.19576241753618007 1103 9 -0.0012875021291662847 1103 10-11 0.23175699687654117 1103 12-13 0.20223344442005953 1103 14-15 0.48966254869588965 1103 16-17 0.3188745886365112 1103 18-19 0.20580590541368338 1103 20-21 0.1713329075898784 1103 22-23 0.23213902892310756 1103 24-25 0.2963571285958295 1103 26-27 0.30230188576257433 1103 28-29 0.32543598821872877 1103 30-31 0.45682005319291363 1103 32-33 0.3813881354582236 1103 34-35 0.403173483751722 1103 36-37 0.448429355588722 1103 38-39 0.5644489066661507 1103 40-41 0.7500986289800622 1103 42-43 0.6013767233287837 1103 44-45 0.6507823479321218 1103 46-47 0.7655265852583 1103 48-49 0.9006358782494388 1103 50-51 0.7360016016878177 1103 52-53 0.7418239204859276 1103 54-55 0.9534796933642866 1103 56-57 0.8253214556574449 1103 58-59 0.9860361918159413 1103 60-61 1.0655680531828295 1103 62-63 1.1680458924867452 1103 64-65 0.9231207805116597 1103 66-67 1.0308736571315222 1103 68-69 0.5751624828112654 1103 70-71 0.636327469935317 1103 72-73 0.47208919779878755 1103 74-75 0.6940624384384577 1103 76-77 0.561951439806883 1103 78-79 0.49474635745523443 1103 80-81 0.6343092584583374 1103 82-83 0.6341210706473674 1103 84-85 0.50829723292469 1103 86-87 0.4320487174126164 1103 88-89 0.6324764280179664 1103 90-91 0.7950113086334056 1103 92-93 0.9126380309065496 1103 94-95 0.9120930730660781 1103 96-97 1.0380426238276463 1103 98-99 0.861628869042459 1103 100-101 1.077788061860236 1104 1 0.09463311920416118 1104 2 0.14448161441609386 1104 3 0.11410416207723273 1104 4 0.1316879558342805 1104 5 0.011547977406060284 1104 6 0.0995433514669557 1104 7 0.2200895312360629 1104 8 0.15966954951218781 1104 9 0.09480738861248028 1104 10-11 0.1685676136157923 1104 12-13 0.23331376527364256 1104 14-15 0.27551047825475194 1104 16-17 0.33690047942239687 1104 18-19 0.48847279542209066 1104 20-21 0.28978514932992283 1104 22-23 0.33736301329761176 1104 24-25 0.19613146915826718 1104 26-27 0.33815613631407615 1104 28-29 0.45202808824211616 1104 30-31 0.2566057715421266 1104 32-33 0.31404744027545206 1104 34-35 0.1957374679998125 1104 36-37 0.447237848474451 1104 38-39 0.5917603356529568 1104 40-41 0.7274963395165273 1104 42-43 0.5213466354037735 1104 44-45 0.4582101463935899 1104 46-47 0.3609403890420424 1104 48-49 0.1072786094239433 1104 50-51 0.40330879560955424 1104 52-53 0.38991845725198004 1104 54-55 0.2266097857139897 1104 56-57 0.06436085440462591 1104 58-59 0.30503298707266424 1104 60-61 0.3323566096577366 1104 62-63 0.49642936182677033 1104 64-65 0.38480134282716705 1104 66-67 0.15988195659486593 1104 68-69 0.5439920878124269 1104 70-71 0.6358581362311497 1104 72-73 0.7036066152940279 1104 74-75 0.6515948493282551 1104 76-77 0.6463840991116996 1104 78-79 0.7516188567125184 1104 80-81 0.687016855659401 1104 82-83 0.7025631987991687 1104 84-85 0.891997289181532 1104 86-87 0.7891105303997286 1104 88-89 0.8977684894833011 1104 90-91 0.9538467921577407 1104 92-93 1.2280539838277988 1104 94-95 1.2179722283286232 1104 96-97 0.8138528170603934 1104 98-99 0.6285438453703414 1104 100-101 0.782158396177536 1105 1 0.04781214969811742 1105 2 0.122746789035034 1105 3 0.2172272864812328 1105 4 0.025457244958644765 1105 5 0.16019419696618797 1105 6 0.028967625373283568 1105 7 -0.019182440466622097 1105 8 0.05558063597925411 1105 9 0.10871231474637 1105 10-11 0.19815823479795824 1105 12-13 0.24848152924331401 1105 14-15 0.31570040662793275 1105 16-17 0.3079313755345652 1105 18-19 0.34812261307988734 1105 20-21 0.31191756298014894 1105 22-23 0.029846005354983163 1105 24-25 0.25781858394702795 1105 26-27 0.3021385997770878 1105 28-29 0.32063049692629875 1105 30-31 0.4317197925457492 1105 32-33 0.41626113863848246 1105 34-35 0.14842903394919205 1105 36-37 0.3350722567686546 1105 38-39 0.521400921950935 1105 40-41 0.37063573304970276 1105 42-43 0.5452948544825276 1105 44-45 0.6786020069543497 1105 46-47 0.6130006884062098 1105 48-49 0.7756564797522785 1105 50-51 0.7609980918021506 1105 52-53 0.636200778436276 1105 54-55 0.5644866948231808 1105 56-57 0.4962696741615673 1105 58-59 0.5057602232401024 1105 60-61 0.6491720037880597 1105 62-63 0.3326732647116444 1105 64-65 0.4676307089099865 1105 66-67 0.32549651228616483 1105 68-69 0.30153026999620636 1105 70-71 0.3274956819690331 1105 72-73 0.2872357278953004 1105 74-75 0.3113963903255481 1105 76-77 0.19445114985980894 1105 78-79 0.2649068355321802 1105 80-81 0.2718082665341299 1105 82-83 0.14988656145145995 1105 84-85 0.25355583519666425 1105 86-87 0.09678596431933784 1105 88-89 0.29207855958679474 1105 90-91 0.42350517091159645 1105 92-93 0.5321282734243908 1105 94-95 0.5199991668380157 1105 96-97 0.488135101684847 1105 98-99 0.2325306960142477 1105 100-101 0.23862933567404454 1106 1 0.015516143817208672 1106 2 0.031079521785081 1106 3 -8.651649075588352E-4 1106 4 -0.03888684602251402 1106 5 -0.07306178123984353 1106 6 0.004025641718754969 1106 7 -0.11939432625008806 1106 8 -0.058976061485317643 1106 9 -0.20742703979476573 1106 10-11 -0.005210316739294285 1106 12-13 -0.10626860408617489 1106 14-15 0.06238302478668345 1106 16-17 0.03719900025168954 1106 18-19 -0.006516532458256563 1106 20-21 0.017087864376868822 1106 22-23 -0.03684180184336583 1106 24-25 -0.1323698070182715 1106 26-27 -0.0823432890333251 1106 28-29 -0.04522850533670919 1106 30-31 -0.07810623048781906 1106 32-33 0.03328262991080777 1106 34-35 -0.06145464507987697 1106 36-37 -0.08877570437120141 1106 38-39 0.06095149346598561 1106 40-41 -0.12625084319314794 1106 42-43 -0.16396613979511443 1106 44-45 -0.29341753842812324 1106 46-47 -0.3106195315514171 1106 48-49 -0.7074056301688714 1106 50-51 -0.3650062035695143 1106 52-53 -0.4775905356880017 1106 54-55 -0.38016066669433 1106 56-57 -0.5240684782520617 1106 58-59 -0.39439833074753494 1106 60-61 -0.3711301136283751 1106 62-63 -0.4041617094152272 1106 64-65 -0.4507226358584475 1106 66-67 -0.26938651811295244 1106 68-69 -0.3300724225387768 1106 70-71 -0.4934536239969205 1106 72-73 -0.3390669372531008 1106 74-75 -0.19502216337379963 1106 76-77 -0.24027649763143444 1106 78-79 -0.5557844598538892 1106 80-81 -0.5627420887709391 1106 82-83 -0.4774985950124915 1106 84-85 -0.4668904222085324 1106 86-87 -0.3682009910116797 1106 88-89 -0.3257400258233538 1106 90-91 -0.19153583691520026 1106 92-93 -0.07792837359119176 1106 94-95 -0.19474923681161016 1106 96-97 -0.18165892227121816 1106 98-99 -0.33834870205715717 1106 100-101 0.01895855140836389 1107 1 0.04548130061163391 1107 2 0.029378353512889532 1107 3 0.15812233253044639 1107 4 -0.03253308500591601 1107 5 0.15643196747915056 1107 6 -0.003906311550359476 1107 7 -0.03447938466369749 1107 8 0.06885751096805137 1107 9 0.1976969610250734 1107 10-11 0.17965338483890037 1107 12-13 0.018398335860531745 1107 14-15 0.17814445130850487 1107 16-17 0.21919326137076922 1107 18-19 0.08506985819555979 1107 20-21 0.08816800374969347 1107 22-23 0.16313770215335666 1107 24-25 0.2482307258778107 1107 26-27 0.3105445978287378 1107 28-29 0.29278133274486606 1107 30-31 0.08809586010451653 1107 32-33 0.16631193919550213 1107 34-35 0.2159997513128289 1107 36-37 0.24015440425761625 1107 38-39 0.4146510220579174 1107 40-41 0.520654260311666 1107 42-43 0.26394941667137317 1107 44-45 0.2753158085692178 1107 46-47 0.44691274965375527 1107 48-49 0.3127931810629363 1107 50-51 0.3890110130732438 1107 52-53 0.2758425448612982 1107 54-55 0.27450217405172594 1107 56-57 0.039520371922158404 1107 58-59 0.06555247884459448 1107 60-61 0.28930235204081356 1107 62-63 0.38591822499756745 1107 64-65 0.19858050003005445 1107 66-67 0.20174486126806812 1107 68-69 0.3485297500375708 1107 70-71 0.5436953819469252 1107 72-73 0.6264997772375764 1107 74-75 0.5992696997128988 1107 76-77 0.30659786186846105 1107 78-79 0.3256359131733646 1107 80-81 0.7407422317865482 1107 82-83 0.9736594291727911 1107 84-85 0.5967599159755252 1107 86-87 0.5301490807243319 1107 88-89 0.6112450120942512 1107 90-91 0.37095647708356694 1107 92-93 0.2810181318402485 1107 94-95 0.37292856601683866 1107 96-97 0.23294448006532775 1107 98-99 0.5258755242187192 1107 100-101 0.7166834950443608 1108 1 0.001851547543914478 1108 2 -0.11703885600081776 1108 3 -0.07271390440335779 1108 4 0.03847888287774026 1108 5 -0.03119111993184731 1108 6 -0.036931610747444665 1108 7 -0.016489686133176917 1108 8 -0.1555781805938352 1108 9 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-0.7157476442015778 2316 76-77 -0.7419262545241665 2316 78-79 -0.8793475974835232 2316 80-81 -1.0430414995051365 2316 82-83 -1.0525817386378336 2316 84-85 -0.9407004144328468 2316 86-87 -1.0310022988257757 2316 88-89 -0.8464430227411839 2316 90-91 -0.9160629721840259 2316 92-93 -1.1034982001840348 2316 94-95 -1.0841855464525239 2316 96-97 -1.0648765571512584 2316 98-99 -1.4901001049594562 2316 100-101 -1.7719701277556048 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 6.0 5 15.0 6 34.0 7 87.0 8 148.0 9 254.0 10 477.0 11 787.0 12 1336.0 13 1974.0 14 2310.0 15 3073.0 16 4110.0 17 5994.0 18 5243.0 19 2780.0 20 2361.0 21 2295.0 22 2406.0 23 2621.0 24 3159.0 25 3626.0 26 4447.0 27 5264.0 28 6541.0 29 8024.0 30 10086.0 31 12597.0 32 15754.0 33 20244.0 34 27952.0 35 45517.0 36 63327.0 37 125646.0 38 217708.0 39 48996.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.49404714684111 22.706275057819873 14.899677185102917 21.900000610236102 2 13.846453541284292 23.754561863279548 41.17121559388687 21.227769001549284 3 16.81540079091558 30.289875580671715 31.041078006673633 21.85364562173907 4 10.562024792571053 18.981669565270316 41.89737573663333 28.558929905525304 5 10.094292109255294 41.669595701850085 36.46656411239583 11.769548076498792 6 26.27063067833412 42.198647926927805 18.591232979262433 12.939488415475653 7 25.08714830656119 34.958225111189215 24.561443550264524 15.393183031985064 8 23.45130117633096 36.9311014846024 22.53409473032335 17.083502608743284 9 26.27686914288062 17.024465432059316 23.200393175424104 33.49827224963596 10-11 21.78934923487282 29.112339872582776 30.187945728236443 18.91036516430796 12-13 23.585808558260414 27.630692028307415 29.86946393330625 18.914035480125925 14-15 21.147862260789122 29.474386538306458 28.10664122590393 21.271109975000492 16-17 18.21579806666697 32.59977450027161 29.507521983180336 19.676905449881087 18-19 17.85342042838471 31.64141610102052 32.4336250195903 18.071538451004468 20-21 18.72059548517069 30.81129072506284 32.25549746504933 18.21261632471714 22-23 19.018125796450917 30.1995929779181 31.685946326340414 19.096334899290564 24-25 18.814982417752972 30.779355353113296 31.885340316526094 18.520321912607635 26-27 18.309917018982848 31.0375784485205 32.48082580833645 18.171678724160202 28-29 18.127455030573046 29.80965358153916 33.48557090611408 18.577320481773715 30-31 18.54473220324541 30.874513375192386 32.439320729969545 18.14143369159266 32-33 17.67108665732912 31.596301602167365 32.591883640303195 18.140728100200317 34-35 18.148854845679576 30.67746087972684 32.67600318926848 18.497681085325105 36-37 19.027179184814653 29.86165053160127 31.826235806531372 19.284934477052705 38-39 18.421819426745532 30.775434125187818 32.471156589383185 18.331589858683454 40-41 17.846592296672522 30.332032631908092 33.87664055373515 17.94473451768424 42-43 17.963374366621526 30.68328793803923 32.069264596235485 19.28407309910376 44-45 17.767428979661087 31.15430352174452 31.935053641382954 19.14321385721144 46-47 18.35368971457442 30.364588722980745 31.884773650026478 19.396947912418355 48-49 18.439868488831365 30.64931547082353 31.4939726583989 19.4168433819462 50-51 18.856674181205637 30.54298694174495 31.038824779870673 19.56151409717874 52-53 18.202367728289484 30.951504177001382 31.957864783845885 18.888263310863245 54-55 18.741117755359777 30.60018867637437 31.36927313948357 19.28942042878228 56-57 19.011009661508638 31.32287120042666 30.2181770873355 19.447942050729196 58-59 18.023641079209103 30.391804060690486 31.51443959138988 20.070115268710527 60-61 19.121597615354126 31.30882396575191 30.374715936972436 19.194862481921533 62-63 19.128729955128705 31.052946305079175 31.33778546681213 18.48053827297999 64-65 18.70356309788819 30.398472936310977 31.357072103394813 19.540891862406024 66-67 18.612002501472126 31.274944348331513 30.699864427514367 19.413188722681994 68-69 18.315142883661768 30.908092201462843 31.264065074793102 19.512699840082288 70-71 19.052556941378036 30.204074490536527 31.418068169887604 19.32530039819784 72-73 18.793874347899852 30.420714436243873 30.374686075165453 20.410725140690822 74-75 18.715545797176866 30.835056518967214 30.11824172680699 20.33115595704893 76-77 18.46980039714755 30.313258706183227 31.419554752621288 19.79738614404793 78-79 17.906794498723226 29.834198603737633 31.58421021767954 20.674796679859597 80-81 18.425301085658205 31.428549691496567 29.969796334477067 20.176352888368164 82-83 18.930848902867105 30.462878853583238 30.278311597609907 20.32796064593975 84-85 18.159589109359082 30.098682834086393 30.39326300028986 21.348465056264658 86-87 18.401923766853717 30.565696836098198 30.869160850400014 20.16321854664807 88-89 18.701066363040912 29.669470307727153 31.137708972263546 20.491754356968393 90-91 18.237060495640726 29.208691836617888 31.731155922696836 20.823091745044547 92-93 18.638785080243363 30.530627068649007 30.637750469618613 20.192837381489014 94-95 17.963044538429525 29.284772514765372 31.336365911869585 21.415817034935518 96-97 18.32316259012729 29.231332229146428 30.57628970374873 21.86921547697755 98-99 18.03457416052441 29.714803498499688 30.725080493490527 21.52554184748538 100-101 18.10077958754212 29.492937151412836 30.942101150855933 21.46418211018911 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2234.0 1 1334.0 2 764.0 3 1067.0 4 752.5 5 768.0 6 1825.0 7 3102.0 8 3684.5 9 3301.0 10 2307.0 11 1640.5 12 1511.0 13 1447.5 14 1276.5 15 1148.5 16 1084.5 17 1037.5 18 1076.5 19 1140.5 20 1221.0 21 1375.0 22 1574.0 23 1874.0 24 2154.5 25 2655.5 26 4136.5 27 5782.0 28 5992.0 29 6781.0 30 10084.5 31 13189.5 32 17773.0 33 23483.5 34 27453.5 35 32602.0 36 40402.0 37 44019.0 38 40548.5 39 36798.0 40 32660.5 41 28118.0 42 23336.5 43 22616.5 44 23507.5 45 21925.0 46 18701.0 47 15544.0 48 21205.5 49 20294.5 50 13429.5 51 11005.0 52 7886.0 53 6468.0 54 5886.5 55 5213.0 56 4484.0 57 4084.5 58 3719.0 59 3086.5 60 2723.0 61 2477.0 62 2011.0 63 1462.5 64 963.5 65 803.0 66 655.5 67 452.5 68 289.5 69 176.5 70 143.0 71 126.5 72 109.0 73 84.0 74 89.5 75 96.5 76 63.0 77 26.5 78 5.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2630806146561313 2 0.020541285701895732 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.043153437317886E-4 12-13 0.0 14-15 0.0 16-17 9.129460311953659E-4 18-19 0.0 20-21 1.521576718658943E-4 22-23 7.607883593294716E-5 24-25 0.002434522749854309 26-27 0.0022062862420554677 28-29 0.0025106015857872563 30-31 0.0011411825389942073 32-33 0.001445497882725996 34-35 0.0013694190467930488 36-37 7.607883593294716E-5 38-39 7.607883593294716E-5 40-41 5.325518515306301E-4 42-43 0.003499626452915569 44-45 0.003727862960714411 46-47 0.013542032796064595 48-49 0.01255300792893628 50-51 0.006466701054300508 52-53 0.006542779890233456 54-55 4.5647301559768297E-4 56-57 0.0026627592576531503 58-59 0.001445497882725996 60-61 0.0012933402108601018 62-63 0.0 64-65 6.086306874635773E-4 66-67 0.0 68-69 0.0 70-71 3.043153437317886E-4 72-73 0.001445497882725996 74-75 0.0 76-77 0.19559868718360715 78-79 0.10263034967354571 80-81 0.0012172613749271545 82-83 0.0 84-85 9.89024867128313E-4 86-87 0.12058495495372124 88-89 0.5746995266374828 90-91 0.30439142256772156 92-93 0.28818663051400384 94-95 0.0025106015857872563 96-97 0.0026627592576531503 98-99 0.0025866804217202035 100-101 9.89024867128313E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 657213.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.251331912201792 #Duplication Level Percentage of deduplicated Percentage of total 1 66.45223641817492 14.12198532430739 2 12.450643968085716 5.291855349728855 3 5.6266438810499775 3.5872103000385693 4 3.2218496171680306 2.738743823425524 5 2.130421232730599 2.263714436478003 6 1.5760109048546678 2.009539850178961 7 1.148925080039102 1.7091331752714778 8 0.8679267255274548 1.475567913572352 9 0.6729149832572915 1.2870305692104973 >10 4.547688800357421 18.911845588607747 >50 0.6255300887392863 9.366030297171289 >100 0.6226672508045527 25.615398536316043 >500 0.04007973108627006 5.5909629531775344 >1k 0.015745608641034664 5.199311123573025 >5k 7.157094836833938E-4 0.8316707589427014 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5468 0.8319981497627101 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3770 0.5736344229344216 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3190 0.48538297325220287 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2616 0.39804446960117956 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2203 0.3352033511205652 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1938 0.29488156807610316 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1674 0.2547119427035071 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1450 0.22062862420554677 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1341 0.2040434379721643 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1289 0.19613123903513777 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1281 0.1949139776602106 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 1274 0.19384887395714936 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1272 0.19354455861341757 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1222 0.18593667502012284 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1186 0.18045899883295066 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1151 0.17513348031764436 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1097 0.16691696603688605 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1074 0.1634173395839705 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1058 0.16098281683411617 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1036 0.1576353480530665 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1033 0.15717887503746883 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1015 0.15444003694388272 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1014 0.15428787927201681 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 997 0.15170119885029662 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 985 0.1498753067879059 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 969 0.14744078403805158 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 944 0.14363684224140422 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 886 0.13481169727318235 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 858 0.1305512824609373 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 853 0.12979049410160787 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 848 0.12902970574227837 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 812 0.12355202955510619 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 793 0.1206610337896542 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 791 0.1203567184459224 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 774 0.11777003802420219 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 769 0.11700924966487274 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 730 0.11107510046210285 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 728 0.11077078511837106 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 719 0.10940136607157801 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 716 0.10894489305598033 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 716 0.10894489305598033 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 673 0.10240211316574688 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 673 0.10240211316574688 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 669 0.1017934824782833 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.521576718658943E-4 0.0 12-13 0.0 0.0 0.0 3.043153437317886E-4 0.0 14-15 0.0 0.0 0.0 6.086306874635773E-4 0.0 16-17 0.0 0.0 0.0 0.003423547616982622 0.0 18-19 0.0 0.0 0.0 0.005934149202769879 0.0 20-21 0.0 0.0 0.0 0.012096534913338598 0.0 22-23 0.0 0.0 0.0 0.026779750248397398 0.0 24-25 0.0 0.0 0.0 0.03849589098207126 0.0 26-27 0.0 0.0 0.0 0.04602769573943303 0.0 28-29 0.0 0.0 0.0 0.05378773700459364 0.0 30-31 0.0 0.0 0.0 0.07052508090984201 0.0 32-33 0.0 0.0 0.0 0.1005001422674232 0.0 34-35 0.0 0.0 0.0 0.14257173853834298 0.0 36-37 0.0 0.0 0.0 0.20670619722981742 0.0 38-39 0.0 0.0 0.0 0.2941207797167737 0.0 40-41 0.0 0.0 0.0 0.3890671669610918 0.0 42-43 0.0 0.0 0.0 0.4811225584399578 0.0 44-45 0.0 0.0 0.0 0.5800250451527891 0.0 46-47 0.0 0.0 0.0 0.7067723858170791 0.0 48-49 0.0 0.0 0.0 0.8443229211838476 0.0 50-51 0.0 0.0 0.0 0.9826342449099454 0.0 52-53 0.0 0.0 0.0 1.1042082247307952 0.0 54-55 0.0 0.0 0.0 1.2306512500513533 0.0 56-57 0.0 0.0 0.0 1.3751249594880197 0.0 58-59 0.0 0.0 0.0 1.5426505562123696 0.0 60-61 0.0 0.0 0.0 1.7177840365300139 0.0 62-63 0.0 0.0 0.0 1.8913959401289993 0.0 64-65 0.0 0.0 0.0 2.0728439638290785 0.0 66-67 0.0 0.0 0.0 2.2727030658249303 0.0 68-69 0.0 0.0 0.0 2.506265092139078 0.0 70-71 0.0 0.0 0.0 2.759531536959859 0.0 72-73 0.0 0.0 0.0 3.0112764050619814 0.0 74-75 0.0 0.0 0.0 3.2710856297729958 0.0 76-77 0.0 0.0 0.0 3.5597287333025975 0.0 78-79 0.0 0.0 0.0 3.887780673845466 0.0 80-81 0.0 0.0 0.0 4.258969314362314 0.0 82-83 0.0 0.0 0.0 4.60657351573995 0.0 84-85 0.0 0.0 0.0 4.987880641435881 0.0 86-87 0.0 0.0 0.0 5.394598098333417 0.0 88-89 0.0 0.0 0.0 5.8314427742604 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACTGCG 20 0.0020819688 71.17838 2 GTGTCGA 25 0.005043382 56.93404 9 TAGACTC 55 8.2509905E-7 51.758217 5 TTCGGAC 25 2.8052815E-5 47.562637 92-93 GTACTGC 55 4.9103634E-5 43.256905 1 CATTCCG 120 8.731149E-11 39.53753 9 GATGACC 205 0.0 37.03027 9 CTAGACG 55 0.002593509 34.505478 3 GTCCTAC 85 1.6118564E-5 33.587715 1 GTACACT 115 1.01956175E-7 33.100937 1 GTATAGG 520 0.0 32.02675 1 GTAGTAA 75 3.04582E-4 31.721731 1 TCGGACA 30 0.003925748 31.708424 92-93 AACGGAT 30 0.003973616 31.630022 32-33 TACGAAC 30 0.003973616 31.630022 28-29 CATGGGG 1190 0.0 31.098425 4 ATGTATT 110 2.694349E-6 30.279835 1 ACCGGTG 65 0.0058754236 29.196941 7 AGATAGC 65 0.0058754236 29.196941 6 CATGGGT 330 0.0 28.754564 4 >>END_MODULE