FastQCFastQC Report
Thu 26 May 2016
SRR936333_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936333_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293981
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53830.2346575669109727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35160.1532706678913208No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25700.11203231412989034No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24690.10762948777692578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG19700.029.0714282
TACATGG116200.028.8386122
GTATCAA99500.028.73921
GTACATG121350.027.9730741
ACATGGG121150.026.7611583
GTACCTG22650.026.336811
GGTATCA69350.025.3272631
TCAACGC118050.024.096074
CAACGCA118100.024.0858695
ATCAACG118800.024.0634673
TATCAAC121250.023.5387152
AACGCAG121550.023.5185326
CATGGGA57700.022.6397154
AGTACAT82400.021.8274782
CATGGGG61250.021.7139054
GAGTACA93300.021.3629511
ACGCAGA138600.020.4887897
ATGGGAG24700.020.3117165
CGCAGAG140250.020.0457028
TATACGA1200.00486407419.721322
Produced by FastQC (version 0.11.5)