##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936333_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2293981 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.728195656372044 33.0 31.0 34.0 30.0 34.0 2 31.817710783132032 33.0 31.0 34.0 30.0 34.0 3 31.886706559470195 34.0 31.0 34.0 30.0 34.0 4 35.38438112608605 37.0 35.0 37.0 33.0 37.0 5 35.372352255751025 37.0 35.0 37.0 33.0 37.0 6 35.465683891889256 37.0 35.0 37.0 33.0 37.0 7 35.40989921015039 37.0 35.0 37.0 33.0 37.0 8 35.41197725700431 37.0 35.0 37.0 33.0 37.0 9 37.07928444045526 39.0 37.0 39.0 33.0 39.0 10-11 37.001156068860205 39.0 37.0 39.0 33.0 39.0 12-13 36.90838524817773 39.0 37.0 39.0 33.0 39.0 14-15 38.1451478891935 40.0 38.0 41.0 33.0 41.0 16-17 38.09810412553548 40.0 38.0 41.0 33.0 41.0 18-19 38.060275346657185 40.0 38.0 41.0 33.0 41.0 20-21 37.98320169173154 40.0 38.0 41.0 33.0 41.0 22-23 37.819849205377025 40.0 38.0 41.0 32.5 41.0 24-25 37.97852248994215 40.0 38.0 41.0 33.0 41.0 26-27 37.93124637911125 40.0 38.0 41.0 33.0 41.0 28-29 37.81169591204112 40.0 38.0 41.0 33.0 41.0 30-31 37.66932180344999 40.0 38.0 41.0 32.0 41.0 32-33 37.49383190183354 40.0 37.0 41.0 32.0 41.0 34-35 37.24035138041684 40.0 37.0 41.0 31.5 41.0 36-37 37.041236828029525 40.0 37.0 41.0 31.0 41.0 38-39 36.88469063170096 40.0 37.0 41.0 30.5 41.0 40-41 36.70832212646923 40.0 36.0 41.0 30.0 41.0 42-43 36.51827434490521 40.0 36.0 41.0 30.0 41.0 44-45 36.331814212933764 40.0 36.0 41.0 30.0 41.0 46-47 36.061941881820296 39.5 35.5 41.0 29.0 41.0 48-49 35.8820206008681 39.0 35.0 41.0 28.0 41.0 50-51 35.56962568565302 39.0 35.0 40.5 28.0 41.0 52-53 35.58935884822063 39.0 35.0 40.5 28.0 41.0 54-55 35.6735055782938 39.0 35.0 41.0 28.0 41.0 56-57 35.51004454701238 39.0 35.0 41.0 28.0 41.0 58-59 35.276010350565244 39.0 35.0 41.0 27.5 41.0 60-61 34.9766358134614 38.0 35.0 40.5 26.5 41.0 62-63 34.670991608038605 37.5 34.0 40.0 26.0 41.0 64-65 34.33664468014338 37.0 34.0 40.0 26.0 41.0 66-67 33.97222775602762 36.5 34.0 39.5 26.0 41.0 68-69 33.58994930646766 36.0 34.0 39.0 25.0 41.0 70-71 33.19202970730795 35.5 33.0 39.0 24.5 40.5 72-73 32.79014647462206 35.0 33.0 38.0 23.5 40.0 74-75 32.37963501005457 35.0 33.0 37.0 22.0 39.5 76-77 31.995139018152287 35.0 32.5 37.0 21.5 39.0 78-79 31.609883647684963 35.0 32.0 36.0 20.0 39.0 80-81 31.22384775636764 35.0 32.0 36.0 18.5 37.5 82-83 30.881350150676923 35.0 31.5 35.0 17.5 37.0 84-85 30.57202696970899 35.0 31.0 35.0 14.5 37.0 86-87 30.268153921065604 34.0 31.0 35.0 8.5 36.0 88-89 29.947087835513894 34.0 31.0 35.0 4.5 36.0 90-91 29.600871585248527 34.0 30.5 35.0 2.0 35.5 92-93 29.30204783736221 34.0 30.0 35.0 2.0 35.0 94-95 28.937063558939677 34.0 30.0 35.0 2.0 35.0 96-97 28.52577004779028 34.0 29.0 35.0 2.0 35.0 98-99 28.044914277842757 34.0 29.0 35.0 2.0 35.0 100-101 24.774028860744707 30.0 22.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30099211951051075 1101 2 0.3049725700371049 1101 3 0.3522741475413973 1101 4 0.17191317759073144 1101 5 0.3135346512556936 1101 6 0.2929581854790442 1101 7 0.392961342949377 1101 8 0.3011533151201178 1101 9 0.29643613018244963 1101 10-11 0.5484923396432819 1101 12-13 0.40307102316864274 1101 14-15 0.5848162697867281 1101 16-17 0.5700380205560052 1101 18-19 0.5679347186352146 1101 20-21 0.5481075803417141 1101 22-23 0.5872758905621893 1101 24-25 0.5398869117919673 1101 26-27 0.39399030027869486 1101 28-29 0.3921653139691017 1101 30-31 0.5238036206696464 1101 32-33 0.5507479457968714 1101 34-35 0.4835659753912225 1101 36-37 0.4970464341146581 1101 38-39 0.556986726622064 1101 40-41 0.43125833995252805 1101 42-43 0.44554244762323236 1101 44-45 0.4480304587588009 1101 46-47 0.3457461927026344 1101 48-49 0.47709743182362985 1101 50-51 0.5291118580748204 1101 52-53 0.6096197338934672 1101 54-55 0.6345010168299297 1101 56-57 0.6596993761257011 1101 58-59 0.6904792163432703 1101 60-61 0.5044855846095473 1101 62-63 0.4337059535619403 1101 64-65 0.5176812745116521 1101 66-67 0.5244449055004523 1101 68-69 0.5407186741829051 1101 70-71 0.5925809952166787 1101 72-73 0.5286484630281976 1101 74-75 0.5565881787672708 1101 76-77 0.5732350025848447 1101 78-79 0.6417709315105533 1101 80-81 0.6919050052713551 1101 82-83 0.8604039104893495 1101 84-85 0.7176631657398964 1101 86-87 0.7349672459709389 1101 88-89 0.5973986815481531 1101 90-91 0.7096914946852451 1101 92-93 0.7019449976742358 1101 94-95 0.6690307057309148 1101 96-97 0.6426770615713693 1101 98-99 0.8568557887900674 1101 100-101 0.8869327422795514 1102 1 0.4303591486847935 1102 2 0.33105479014052364 1102 3 0.34721184304735786 1102 4 0.24146902511100876 1102 5 0.36602600729226253 1102 6 0.2463029205382199 1102 7 0.2377664187423676 1102 8 0.29978490208796416 1102 9 0.2994828827498992 1102 10-11 0.4134964376747874 1102 12-13 0.4585617675894511 1102 14-15 0.5677327296641153 1102 16-17 0.5718957776765947 1102 18-19 0.5157113306134136 1102 20-21 0.4924775749257222 1102 22-23 0.5563226245958148 1102 24-25 0.6496817219130762 1102 26-27 0.5885757504134759 1102 28-29 0.4866913529436516 1102 30-31 0.5742047747082708 1102 32-33 0.31452088396189737 1102 34-35 0.27286692123337275 1102 36-37 0.342507143465383 1102 38-39 0.31105335361264963 1102 40-41 0.31317145138809366 1102 42-43 0.33445876540807973 1102 44-45 0.37969835758066495 1102 46-47 0.44686563487611863 1102 48-49 0.4561900061493489 1102 50-51 0.274922187173523 1102 52-53 0.35126587608074544 1102 54-55 0.4431553797919676 1102 56-57 0.5059529207170144 1102 58-59 0.5642413122289724 1102 60-61 0.4891084421524923 1102 62-63 0.5346802142173317 1102 64-65 0.4098623745551109 1102 66-67 0.42724665138543116 1102 68-69 0.5793611549128812 1102 70-71 0.5464125002238944 1102 72-73 0.35148788403194686 1102 74-75 0.4770384885908143 1102 76-77 0.6092225149149826 1102 78-79 0.641093417759496 1102 80-81 0.6741779300508561 1102 82-83 0.6929290495444143 1102 84-85 0.6540005552320487 1102 86-87 0.5929042989714581 1102 88-89 0.4605599524733428 1102 90-91 0.49711280321347573 1102 92-93 0.5112256489935341 1102 94-95 0.4763302986048714 1102 96-97 0.5504798474090684 1102 98-99 0.6313994451814473 1102 100-101 0.9035302846962345 1103 1 0.4096470716224303 1103 2 0.3795513243954609 1103 3 0.4092873371954404 1103 4 0.35247019554362424 1103 5 0.38803945009166085 1103 6 0.396354273206839 1103 7 0.36917423858635345 1103 8 0.385230465521083 1103 9 0.40742206008163606 1103 10-11 0.4747437673609127 1103 12-13 0.522680166979292 1103 14-15 0.6030158464101589 1103 16-17 0.5469389532479525 1103 18-19 0.4776394129807997 1103 20-21 0.6028335387802173 1103 22-23 0.7430185744807929 1103 24-25 0.7548818828164485 1103 26-27 0.7426620089571117 1103 28-29 0.7315181254321601 1103 30-31 0.6886402015278534 1103 32-33 0.7071196277008838 1103 34-35 0.6010449494601602 1103 36-37 0.6784674781961613 1103 38-39 0.5748679184541672 1103 40-41 0.6465610411591811 1103 42-43 0.6018878063752098 1103 44-45 0.6983019632698344 1103 46-47 0.6712352267281929 1103 48-49 0.7373570683535391 1103 50-51 0.7295852235574927 1103 52-53 0.6907337748131823 1103 54-55 0.7971136673858226 1103 56-57 0.6743331684345293 1103 58-59 0.6725849882834964 1103 60-61 0.7497422779603724 1103 62-63 0.6457835672503762 1103 64-65 0.5565851383492628 1103 66-67 0.6334940781512515 1103 68-69 0.6969623754286616 1103 70-71 0.7398157856994274 1103 72-73 0.737979443823825 1103 74-75 0.8132737579919436 1103 76-77 0.6192285687050969 1103 78-79 0.73844082715053 1103 80-81 0.7658552630376896 1103 82-83 0.884625848254398 1103 84-85 0.8937041335895479 1103 86-87 0.9315792557461045 1103 88-89 0.8795893895256235 1103 90-91 1.035333202992632 1103 92-93 0.8891232911518081 1103 94-95 0.9085040772303117 1103 96-97 1.1562689584528059 1103 98-99 1.1226528137223504 1103 100-101 1.0046300731620725 1104 1 0.24151372247580838 1104 2 0.2087872191220903 1104 3 0.27388677582370846 1104 4 0.31462827811054694 1104 5 0.2745474807263122 1104 6 0.2348819123188477 1104 7 0.3648148025457161 1104 8 0.2677743923199287 1104 9 0.3218240123039422 1104 10-11 0.383337930101213 1104 12-13 0.350002653834494 1104 14-15 0.42963715769114685 1104 16-17 0.49625952094501 1104 18-19 0.3512991469581692 1104 20-21 0.442275473464143 1104 22-23 0.5224505180417296 1104 24-25 0.5150119111725502 1104 26-27 0.392547542314162 1104 28-29 0.5064335782492577 1104 30-31 0.4718608651828049 1104 32-33 0.5931219925062479 1104 34-35 0.5313967591831741 1104 36-37 0.6131478161692598 1104 38-39 0.5034755943680125 1104 40-41 0.5682446532017238 1104 42-43 0.7975118382605473 1104 44-45 0.6784217848671901 1104 46-47 0.8791246199651397 1104 48-49 0.7818143311452346 1104 50-51 0.6558191092777363 1104 52-53 0.6443372278602979 1104 54-55 0.8130261480235319 1104 56-57 0.8366005064471551 1104 58-59 0.7216029471473817 1104 60-61 0.6972087376167124 1104 62-63 0.8400203424896446 1104 64-65 0.8414148040779139 1104 66-67 1.0379836144265724 1104 68-69 0.9018263991198481 1104 70-71 0.8095696374929773 1104 72-73 0.9174545145343913 1104 74-75 0.9036585599844145 1104 76-77 0.9148123485841282 1104 78-79 0.9194055814875135 1104 80-81 0.9109234937823167 1104 82-83 1.0387619215993062 1104 84-85 0.8345681860321612 1104 86-87 0.8358172169819831 1104 88-89 0.8793521902027202 1104 90-91 1.0450324989419144 1104 92-93 0.9635855495050016 1104 94-95 0.7232647035802806 1104 96-97 0.829596972771018 1104 98-99 1.0449617694343623 1104 100-101 1.1362731816232632 1105 1 0.39265243166966 1105 2 0.3897468482776567 1105 3 0.35518772945662747 1105 4 0.3876509600445672 1105 5 0.2570984199451871 1105 6 0.3310258682234206 1105 7 0.361734810386956 1105 8 0.3880462987785762 1105 9 0.38634356350838317 1105 10-11 0.30803206416332074 1105 12-13 0.40964045782660463 1105 14-15 0.5264032235806084 1105 16-17 0.49222820211796403 1105 18-19 0.5652438662303183 1105 20-21 0.554259306676407 1105 22-23 0.5888692632130272 1105 24-25 0.6015803317396546 1105 26-27 0.4356945309328353 1105 28-29 0.5843449261350102 1105 30-31 0.6602033658458524 1105 32-33 0.6899307239553778 1105 34-35 0.5390379302951303 1105 36-37 0.5690812117712554 1105 38-39 0.6826940074398209 1105 40-41 0.6575252196158559 1105 42-43 0.7668479852145751 1105 44-45 0.7689684805925623 1105 46-47 0.7590867975230537 1105 48-49 0.8186744918906115 1105 50-51 0.7305022886691219 1105 52-53 0.7226700422881791 1105 54-55 0.7479294586403356 1105 56-57 0.8401959446916365 1105 58-59 0.8199649355930845 1105 60-61 0.6672569381679097 1105 62-63 0.6711858041425316 1105 64-65 0.6435849820048318 1105 66-67 0.6113545395773414 1105 68-69 0.6931047515373692 1105 70-71 0.5892665967070059 1105 72-73 0.5576650748369687 1105 74-75 0.6257149246481006 1105 76-77 0.6470928804045641 1105 78-79 0.64036071346246 1105 80-81 0.592061857039198 1105 82-83 0.6600745217768882 1105 84-85 0.5583876761210682 1105 86-87 0.5908211952571385 1105 88-89 0.754697838892195 1105 90-91 0.8237343128047598 1105 92-93 0.81916032378113 1105 94-95 0.7702302597892761 1105 96-97 0.8528514324751271 1105 98-99 0.8444290265001264 1105 100-101 0.6210617564782552 1106 1 0.2215623842762966 1106 2 0.2646505112975497 1106 3 0.25720305478771266 1106 4 0.2514304701654879 1106 5 0.23882539872890618 1106 6 0.20423975846569675 1106 7 0.19971372698348944 1106 8 0.2733034188565284 1106 9 0.2333072876524227 1106 10-11 0.293290291562883 1106 12-13 0.2858441164172021 1106 14-15 0.3198877432244913 1106 16-17 0.35681162772809927 1106 18-19 0.2524332625185721 1106 20-21 0.28230776307007943 1106 22-23 0.24052360169910258 1106 24-25 0.2746214798106976 1106 26-27 0.32271672165790477 1106 28-29 0.33205719968773906 1106 30-31 0.40821793819824137 1106 32-33 0.4740705565283534 1106 34-35 0.3853937348750094 1106 36-37 0.44325957913626723 1106 38-39 0.4092132526894119 1106 40-41 0.44893683320490396 1106 42-43 0.4422266892053628 1106 44-45 0.46771277788626975 1106 46-47 0.3960635880620913 1106 48-49 0.4654665811850265 1106 50-51 0.6061965793188975 1106 52-53 0.5530372301342155 1106 54-55 0.5885139029863566 1106 56-57 0.5665269452674409 1106 58-59 0.4954875913932355 1106 60-61 0.6684971383716558 1106 62-63 0.6634445187636047 1106 64-65 0.5698916215052066 1106 66-67 0.48527822297194945 1106 68-69 0.6178057747025392 1106 70-71 0.6755734576717032 1106 72-73 0.8931491958450763 1106 74-75 0.7349101233016029 1106 76-77 0.7367283501303845 1106 78-79 0.7939451135156155 1106 80-81 0.7258739224154631 1106 82-83 0.6375504928979403 1106 84-85 0.6507493716519157 1106 86-87 0.6635356834530945 1106 88-89 0.5898087925175659 1106 90-91 0.5799319918586647 1106 92-93 0.4678747233913505 1106 94-95 0.47849641561156986 1106 96-97 0.6674957607738392 1106 98-99 0.5845705422019485 1106 100-101 0.6664076837493731 1107 1 0.11039613117456071 1107 2 0.1934207160701611 1107 3 0.26958449306605203 1107 4 0.22120969606470453 1107 5 0.1874525177660331 1107 6 0.2824835375988357 1107 7 0.17825898335509294 1107 8 0.33340986351226576 1107 9 0.27620749868648886 1107 10-11 0.32241589161854023 1107 12-13 0.2774700063548252 1107 14-15 0.3209788589221816 1107 16-17 0.2191130585854566 1107 18-19 0.33285173611612606 1107 20-21 0.39992099096367895 1107 22-23 0.45576211657584054 1107 24-25 0.4786809867794588 1107 26-27 0.42727250654230176 1107 28-29 0.48860621292955386 1107 30-31 0.38143806296343996 1107 32-33 0.4078690537802814 1107 34-35 0.150053065595543 1107 36-37 0.2158477612285239 1107 38-39 0.14846964748544877 1107 40-41 0.28574720402190934 1107 42-43 -0.01705591091987202 1107 44-45 0.2240600332838696 1107 46-47 0.4284022397631233 1107 48-49 0.15742003773751634 1107 50-51 0.17270825576424187 1107 52-53 0.17232017811779343 1107 54-55 0.45933032653868366 1107 56-57 0.3531720833672125 1107 58-59 0.44653638432475873 1107 60-61 0.5628792259855757 1107 62-63 0.5157896638205415 1107 64-65 0.19444288423102307 1107 66-67 0.28619019161831716 1107 68-69 0.406144982976933 1107 70-71 0.4673280551995873 1107 72-73 0.3114877084027583 1107 74-75 0.30211055928406694 1107 76-77 0.3310814495483001 1107 78-79 0.30671789244003733 1107 80-81 0.42355080838749615 1107 82-83 0.49049859243180904 1107 84-85 0.4217518994226097 1107 86-87 0.30545551369821666 1107 88-89 0.5808259303390457 1107 90-91 0.6207154848203302 1107 92-93 0.32211917505063425 1107 94-95 0.46877435443488125 1107 96-97 0.5903668676591138 1107 98-99 0.7346975105004567 1107 100-101 0.8473838902380884 1108 1 0.02759298733660387 1108 2 0.14097562044397804 1108 3 0.089466654979514 1108 4 0.06968925866115683 1108 5 0.14141168539495652 1108 6 0.03915525936158559 1108 7 0.04251468932271507 1108 8 0.11289518177113678 1108 9 0.1424831585105366 1108 10-11 0.09392603270709543 1108 12-13 0.057862673413090704 1108 14-15 -0.036282499968045556 1108 16-17 0.19794910596190363 1108 18-19 0.16151681106033777 1108 20-21 0.23181187235951484 1108 22-23 0.2853215861704399 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2316 58-59 -1.7377517215032015 2316 60-61 -1.6378911521018154 2316 62-63 -1.5804240187441678 2316 64-65 -1.4769553951267156 2316 66-67 -1.4857737072200408 2316 68-69 -1.5393427486745637 2316 70-71 -1.3629409615989871 2316 72-73 -1.341480788920304 2316 74-75 -1.3654246914384487 2316 76-77 -1.6070934268637558 2316 78-79 -1.7027708475115055 2316 80-81 -1.4913814956534885 2316 82-83 -1.6196961250473727 2316 84-85 -1.6876986524206785 2316 86-87 -1.7189888953868682 2316 88-89 -1.6373739693334244 2316 90-91 -1.6837483365531192 2316 92-93 -1.7348981829519907 2316 94-95 -1.7724437624726512 2316 96-97 -2.1153559050990296 2316 98-99 -1.9534039626728195 2316 100-101 -1.8030988627225355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17527.0 3 3967.0 4 3364.0 5 2794.0 6 2754.0 7 2844.0 8 3508.0 9 4244.0 10 4946.0 11 5985.0 12 7870.0 13 11219.0 14 11568.0 15 10253.0 16 12232.0 17 15295.0 18 13961.0 19 10118.0 20 9777.0 21 10273.0 22 11093.0 23 12060.0 24 13753.0 25 16477.0 26 19349.0 27 23333.0 28 28160.0 29 34306.0 30 42295.0 31 52356.0 32 66818.0 33 87620.0 34 122402.0 35 183884.0 36 308731.0 37 508984.0 38 501164.0 39 96606.0 40 91.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.55631201046379 18.049910613779566 12.70998347477974 23.6837939009769 2 15.716325476253278 19.48584919027678 40.036535095272455 24.761290238197486 3 20.379346515332642 24.080342358791384 27.837360391396736 27.702950734479238 4 12.700281755717835 14.818528479212286 36.17589961945758 36.3052901456123 5 13.943214832537553 37.819779506907416 33.48817317182906 14.748832488725974 6 34.04025656574552 34.5957450333219 16.914017866134344 14.449980534798243 7 30.475941778283865 31.567904119359053 19.721281059109305 18.234873043247777 8 27.523037371178404 34.63878073375177 19.02119346596198 18.81698842910785 9 27.360741248423903 15.97144107804001 19.471112343502305 37.19670533003378 10-11 26.130333873310533 26.105943352961997 26.388559516357123 21.37516325737034 12-13 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25.83833246063495 27.797819546122383 23.026185112356906 38-39 23.170029070881487 26.196532298242058 27.738701500626856 22.89473713024959 40-41 23.157432454632072 26.151857976760233 27.836160728953335 22.85454883965436 42-43 23.080216709932873 26.032108760580293 27.55690584962195 23.330768679864885 44-45 23.07568292323366 26.20838865230392 27.491270354296237 23.224658070166175 46-47 23.452474887917326 26.366273860695465 27.156975325382582 23.024275926004627 48-49 23.31297362073536 26.469043086205275 27.084062682793782 23.13392061026559 50-51 23.234524178861705 26.565613940990573 27.076135939381512 23.123725940766214 52-53 23.472529475502768 26.56477906900832 26.863948466450132 23.098742989038783 54-55 23.261722608985554 26.602731336241398 26.912353748041173 23.22319230673187 56-57 23.157194972231622 26.82275221802037 26.782931346232402 23.237121463515606 58-59 23.256531661223388 26.46049156194989 27.056386619061062 23.22659015776566 60-61 23.175083841757225 26.625080162181675 27.188252341787013 23.01158365427409 62-63 23.08467967135087 26.619777907338886 27.23197605173372 23.06356636957652 64-65 23.154997898160566 26.74768660752601 26.951583652223665 23.145731842089756 66-67 23.173441932642557 26.869328243251704 26.73311191244662 23.22411791165911 68-69 23.089978782288227 26.529806657270647 27.145637269570088 23.234577290871037 70-71 23.416480612474196 26.304251935116913 26.9153288067082 23.363938645700692 72-73 23.202794188382548 26.597620927149762 26.841056660904584 23.35852822356311 74-75 23.038605093981428 26.957407908013387 26.72616202371256 23.277824974292628 76-77 23.25904703703582 26.69124258423878 26.805514029848133 23.24419634887727 78-79 23.258281911347122 26.72813795314941 26.731182531915508 23.28239760358796 80-81 23.200831999383183 26.84836425732052 26.486379689962824 23.464424053333474 82-83 23.42733554577715 26.76365584619405 26.69487747922466 23.11413112880414 84-85 23.20072878544812 26.924605138997553 26.47382356523103 23.40084251032329 86-87 23.11927103880125 26.721078678226647 26.857674571018585 23.30197571195352 88-89 23.245051842637785 26.785388413949807 26.542655025687456 23.42690471772495 90-91 23.075741479927146 26.80663146620107 26.708813439285333 23.408813614586457 92-93 23.187626570193423 26.86472690416455 26.584215695876157 23.363430829765868 94-95 23.174306814408414 26.626742108763136 26.767385603678417 23.431565473150037 96-97 23.200382075558466 26.49028738003204 26.829661921817284 23.479668622592207 98-99 22.991987195277492 26.65044180452873 26.621254569400698 23.73631643079308 100-101 22.99965401409345 26.74716313454472 26.51042556266506 23.742757288696772 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 164.0 1 118.0 2 187.0 3 322.5 4 265.0 5 241.5 6 523.5 7 1007.5 8 1561.5 9 1756.5 10 1522.0 11 1427.0 12 1602.5 13 1832.0 14 2030.0 15 2310.0 16 2565.5 17 2699.5 18 2750.0 19 2906.5 20 3075.5 21 3254.5 22 3662.0 23 4224.0 24 4973.0 25 5961.5 26 7796.0 27 10259.5 28 12382.0 29 14820.0 30 18407.0 31 23173.5 32 28585.0 33 33022.5 34 36962.5 35 41466.5 36 48658.5 37 56125.5 38 60966.0 39 64613.5 40 66308.0 41 67443.5 42 68790.0 43 72170.5 44 76886.5 45 81681.5 46 84516.5 47 86661.0 48 92256.5 49 91699.5 50 85165.0 51 82735.5 52 83075.5 53 83966.0 54 84819.5 55 81837.5 56 75030.5 57 67285.0 58 62941.0 59 59175.5 60 51751.5 61 43376.5 62 34940.0 63 26530.5 64 18986.5 65 13620.0 66 9783.0 67 7563.0 68 5993.0 69 4752.5 70 3724.5 71 2374.0 72 1644.5 73 1347.5 74 945.0 75 625.5 76 432.5 77 259.0 78 172.5 79 112.0 80 64.0 81 39.5 82 33.5 83 32.5 84 31.0 85 33.5 86 25.0 87 15.5 88 15.0 89 21.0 90 19.5 91 18.5 92 25.5 93 20.5 94 17.0 95 20.5 96 18.5 97 16.5 98 14.5 99 11.0 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04891060562402217 2 0.012903332677995153 3 0.010767308011705416 4 0.0065388510192543004 5 0.009285168447341107 6 0.006277296978484129 7 0.005623411876558699 8 0.009067206746699297 9 0.014690618623257996 10-11 0.002484763387316634 12-13 0.008871041216121667 14-15 0.009241576107212745 16-17 0.019769126248212168 18-19 0.012445613106647352 20-21 0.0037053489109107707 22-23 0.010309588440357615 24-25 0.02279879388713333 26-27 0.00782482505304098 28-29 0.01854854072461803 30-31 0.014973968834092348 32-33 0.013796975650626575 34-35 0.2680057071091696 36-37 0.4722140244404814 38-39 0.46942411467226625 40-41 0.4906753804848427 42-43 0.48045297672474185 44-45 0.4963205885314656 46-47 0.5019222042379601 48-49 0.509856010141322 50-51 0.5189668092281496 52-53 0.5083738705769577 54-55 0.4952961685384491 56-57 0.4907843613351636 58-59 0.4880816362472052 60-61 0.48426730648597355 62-63 0.4821312818196838 64-65 0.4994156446805793 66-67 0.472889705712471 68-69 0.49930666383025846 70-71 0.48143380437763 72-73 0.49010868006317404 74-75 0.5040146365641215 76-77 0.4907843613351636 78-79 0.4897599413421471 80-81 0.492026743028822 82-83 0.4920049468587578 84-85 0.49167800430779507 86-87 0.4941409715250475 88-89 0.5074802276043263 90-91 0.5315214031851179 92-93 0.518639866677187 94-95 0.4908061575052278 96-97 0.464825122788724 98-99 0.4554745658311904 100-101 0.4643020147071837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2293981.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.733429654389376 #Duplication Level Percentage of deduplicated Percentage of total 1 68.92471383353435 30.14314123887793 2 14.601440632287416 12.771421534897689 3 6.085010842645103 7.983551808990487 4 3.089378107694378 5.404364005946506 5 1.844471778356383 4.033253838412768 6 1.154850486279284 3.030334350181946 7 0.7861339362648665 2.406623324039743 8 0.5803310022899416 2.0303892051926753 9 0.43777861776407057 1.723100434576272 >10 2.2569591866611685 17.669996685759116 >50 0.15356883976710162 4.628524755081164 >100 0.08086741609140699 6.327910146125329 >500 0.0037960483078766033 1.0821505321718525 >1k 5.993760486120686E-4 0.5300659025773367 >5k 9.989600810201143E-5 0.2351722371691917 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5383 0.2346575669109727 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3516 0.1532706678913208 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2570 0.11203231412989034 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2469 0.10762948777692578 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.3592340128362004E-5 0.0 3 0.0 0.0 0.0 4.3592340128362004E-5 0.0 4 0.0 0.0 0.0 4.3592340128362004E-5 0.0 5 0.0 0.0 0.0 4.3592340128362004E-5 0.0 6 0.0 0.0 0.0 4.3592340128362004E-5 0.0 7 0.0 0.0 0.0 4.3592340128362004E-5 0.0 8 0.0 0.0 0.0 4.3592340128362004E-5 0.0 9 0.0 0.0 0.0 4.3592340128362004E-5 0.0 10-11 0.0 0.0 0.0 4.3592340128362004E-5 0.0 12-13 0.0 0.0 0.0 4.3592340128362004E-5 0.0 14-15 0.0 0.0 0.0 4.3592340128362004E-5 0.0 16-17 0.0 0.0 0.0 1.30777020385086E-4 0.0 18-19 0.0 0.0 0.0 2.8335021083435304E-4 4.3592340128362004E-5 20-21 0.0 0.0 0.0 5.23108081540344E-4 4.3592340128362004E-5 22-23 0.0 0.0 0.0 0.0012423816936583173 6.5388510192543E-5 24-25 0.0 0.0 0.0 0.0017218974350702992 8.718468025672401E-5 26-27 0.0 0.0 0.0 0.002005247645904652 1.08980850320905E-4 28-29 0.0 0.0 0.0 0.002680928917894263 1.30777020385086E-4 30-31 0.0 0.0 0.0 0.004490011033221287 1.30777020385086E-4 32-33 0.0 0.0 0.0 0.008195359944132057 1.30777020385086E-4 34-35 0.0 0.0 0.0 0.013208479058893687 1.30777020385086E-4 36-37 0.0 0.0 0.0 0.021534616023410832 1.30777020385086E-4 38-39 0.0 0.0 0.0 0.035113629973395594 1.30777020385086E-4 40-41 0.0 0.0 0.0 0.050392745188386476 1.30777020385086E-4 42-43 0.0 0.0 0.0 0.06687064975690732 1.30777020385086E-4 44-45 0.0 0.0 0.0 0.08725006876691654 1.30777020385086E-4 46-47 0.0 0.0 0.0 0.11310032646303522 1.30777020385086E-4 48-49 0.0 0.0 0.0 0.1427431177503214 1.7436936051344802E-4 50-51 0.0 0.0 0.0 0.17526300348607943 1.7436936051344802E-4 52-53 0.0 0.0 0.0 0.20669308071862846 2.1796170064181E-4 54-55 0.0 0.0 0.0 0.24130539878054788 2.61554040770172E-4 56-57 0.0 0.0 0.0 0.2834373955146098 2.61554040770172E-4 58-59 0.0 0.0 0.0 0.33278392453991557 2.61554040770172E-4 60-61 0.0 0.0 0.0 0.3852691020544634 2.61554040770172E-4 62-63 0.0 0.0 0.0 0.4400864697658786 2.61554040770172E-4 64-65 0.0 0.0 0.0 0.4951435953479998 2.61554040770172E-4 66-67 0.0 0.0 0.0 0.5602269591596443 2.61554040770172E-4 68-69 0.0 0.0 0.0 0.6371674394862032 2.61554040770172E-4 70-71 0.0 0.0 0.0 0.724090565702157 2.61554040770172E-4 72-73 0.0 0.0 0.0 0.8125830161627319 3.05146380898534E-4 74-75 0.0 0.0 0.0 0.9036692108609443 3.05146380898534E-4 76-77 0.0 0.0 0.0 1.0042367395370755 3.05146380898534E-4 78-79 0.0 0.0 0.0 1.124965725522574 3.05146380898534E-4 80-81 0.0 0.0 0.0 1.2657035956269906 3.05146380898534E-4 82-83 0.0 0.0 0.0 1.412043081437902 3.05146380898534E-4 84-85 0.0 0.0 0.0 1.5594069872418297 3.05146380898534E-4 86-87 0.0 0.0 0.0 1.7149444568198255 3.05146380898534E-4 88-89 0.0 0.0 0.0 1.8990131130118342 3.05146380898534E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1970 0.0 29.071428 2 TACATGG 11620 0.0 28.838612 2 GTATCAA 9950 0.0 28.7392 1 GTACATG 12135 0.0 27.973074 1 ACATGGG 12115 0.0 26.761158 3 GTACCTG 2265 0.0 26.33681 1 GGTATCA 6935 0.0 25.327263 1 TCAACGC 11805 0.0 24.09607 4 CAACGCA 11810 0.0 24.085869 5 ATCAACG 11880 0.0 24.063467 3 TATCAAC 12125 0.0 23.538715 2 AACGCAG 12155 0.0 23.518532 6 CATGGGA 5770 0.0 22.639715 4 AGTACAT 8240 0.0 21.827478 2 CATGGGG 6125 0.0 21.713905 4 GAGTACA 9330 0.0 21.362951 1 ACGCAGA 13860 0.0 20.488789 7 ATGGGAG 2470 0.0 20.311716 5 CGCAGAG 14025 0.0 20.045702 8 TATACGA 120 0.004864074 19.72132 2 >>END_MODULE