FastQCFastQC Report
Thu 26 May 2016
SRR936333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936333_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293981
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.23217280352365605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36710.16002748061121694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27020.11778650302683413No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26000.11334008433374122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG19800.030.9130212
GTATCAA106200.028.4467261
GTACCTG22800.027.5436941
TACATGG117750.027.2397252
GTACATG122550.026.9031431
ACATGGG121750.025.520823
TCAACGC124000.024.1778494
ATCAACG124400.024.138243
CAACGCA124450.024.052835
GGTATCA75000.023.6608681
AACGCAG127450.023.5238826
TATCAAC128500.023.4470142
CATGGGG60650.021.900274
AGTACAT86700.021.7811552
GAGTACA95500.021.720321
GTGTAGC12300.021.2735861
GTGTACG3201.8189894E-1120.8142681
ACGCAGA144400.020.6640387
CGCAGAG147650.020.0164178
CATGGGA58550.019.7690224
Produced by FastQC (version 0.11.5)