##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936333_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2293981 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49389467480332 34.0 31.0 34.0 31.0 34.0 2 32.62195850793882 34.0 31.0 34.0 31.0 34.0 3 32.71142350350766 34.0 31.0 34.0 31.0 34.0 4 36.192318942484704 37.0 35.0 37.0 35.0 37.0 5 36.15741804313113 37.0 35.0 37.0 35.0 37.0 6 36.192557828508605 37.0 37.0 37.0 35.0 37.0 7 36.142344247838146 37.0 36.0 37.0 35.0 37.0 8 36.12013133500234 37.0 36.0 37.0 35.0 37.0 9 37.88705704188483 39.0 38.0 39.0 35.0 39.0 10-11 37.82886693481768 39.0 38.0 39.0 35.0 39.0 12-13 37.77553584794295 39.0 38.0 39.0 35.0 39.0 14-15 39.12528547533742 40.5 38.5 41.0 36.0 41.0 16-17 39.05553925686394 40.0 38.0 41.0 36.0 41.0 18-19 39.08965069893779 40.0 38.5 41.0 36.0 41.0 20-21 39.042478991761485 40.0 39.0 41.0 35.5 41.0 22-23 38.93002579358765 40.0 38.0 41.0 35.0 41.0 24-25 39.08071165367106 40.0 39.0 41.0 35.5 41.0 26-27 38.99916607853335 40.0 38.5 41.0 35.0 41.0 28-29 38.920772665510306 40.0 38.0 41.0 35.0 41.0 30-31 38.8195357764515 40.0 38.0 41.0 35.0 41.0 32-33 38.670874562605356 40.0 38.0 41.0 35.0 41.0 34-35 38.544137462341666 40.0 38.0 41.0 34.0 41.0 36-37 38.403941880948445 40.0 38.0 41.0 34.0 41.0 38-39 38.27486605163687 40.0 38.0 41.0 34.0 41.0 40-41 38.14388807056379 40.0 38.0 41.0 33.0 41.0 42-43 38.17328805251656 40.0 38.0 41.0 34.0 41.0 44-45 38.042422321719314 40.0 38.0 41.0 33.0 41.0 46-47 37.84498171519293 40.0 37.5 41.0 33.0 41.0 48-49 37.611976080011125 40.0 37.0 41.0 33.0 41.0 50-51 37.41740472131199 40.0 37.0 41.0 32.5 41.0 52-53 37.20914209838704 40.0 36.5 41.0 32.0 41.0 54-55 37.01646308317288 39.5 36.0 41.0 31.5 41.0 56-57 36.76347036004222 39.0 35.5 41.0 31.0 41.0 58-59 36.505005272493534 39.0 35.0 41.0 31.0 41.0 60-61 36.224288910849744 38.5 35.0 40.5 31.0 41.0 62-63 35.92526725373924 38.0 35.0 40.0 30.0 41.0 64-65 35.55329991835154 37.0 35.0 40.0 29.5 41.0 66-67 35.20143344692044 37.0 34.5 40.0 29.0 41.0 68-69 34.842690501795786 36.0 34.0 39.0 29.0 41.0 70-71 34.473153003446846 36.0 34.0 39.0 29.0 40.5 72-73 34.07105486052413 35.0 34.0 38.5 28.5 40.0 74-75 33.650438909476584 35.0 34.0 37.0 28.0 39.5 76-77 32.93164197959791 35.0 32.5 36.5 26.5 39.0 78-79 32.98164566315066 35.0 33.0 36.0 27.0 39.0 80-81 32.821432261208784 35.0 33.0 36.0 27.0 37.5 82-83 32.545742096381794 35.0 33.0 35.5 27.0 37.0 84-85 32.261596107378395 35.0 33.0 35.0 26.0 37.0 86-87 31.878670093605834 35.0 33.0 35.0 25.5 36.0 88-89 31.396721245729587 35.0 32.0 35.0 24.5 36.0 90-91 31.194280161867077 35.0 32.0 35.0 24.0 36.0 92-93 30.927580917191555 34.0 32.0 35.0 23.0 35.0 94-95 30.780505156755876 34.0 32.0 35.0 21.5 35.0 96-97 30.54320153479911 34.0 31.5 35.0 19.5 35.0 98-99 30.119344275301323 34.0 31.0 35.0 9.5 35.0 100-101 26.804477892362666 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18592546244267538 1101 2 0.17655214737636982 1101 3 0.19843747386311605 1101 4 0.15075737020833202 1101 5 0.1536892543163475 1101 6 0.17500498870145975 1101 7 0.07642836703950451 1101 8 -0.05058775117532832 1101 9 0.19342479390594036 1101 10-11 0.11463779896259751 1101 12-13 0.21711382638161325 1101 14-15 0.4191677207558868 1101 16-17 0.3587447697070516 1101 18-19 0.35326536619741944 1101 20-21 0.24657395673065707 1101 22-23 0.367423898573044 1101 24-25 0.29092783931634614 1101 26-27 0.35189905909875563 1101 28-29 0.3723868424006511 1101 30-31 0.24923546552092546 1101 32-33 0.34766888558501563 1101 34-35 0.2940429929996853 1101 36-37 0.3821442299986728 1101 38-39 0.4178427704918377 1101 40-41 0.3979553482321947 1101 42-43 0.3307953897774496 1101 44-45 0.3447149898547508 1101 46-47 0.41936681966561906 1101 48-49 0.2945906234759832 1101 50-51 0.34044292719973157 1101 52-53 0.41020296251417676 1101 54-55 0.37967029296449084 1101 56-57 0.38102300883224416 1101 58-59 0.2991320175019112 1101 60-61 0.30759544829542307 1101 62-63 0.2606976783467019 1101 64-65 0.32757430943131993 1101 66-67 0.3581179717338543 1101 68-69 0.4441879637415056 1101 70-71 0.34559413118010696 1101 72-73 0.29311780362462514 1101 74-75 0.14411396251394848 1101 76-77 0.10953368052147283 1101 78-79 0.10814501940653543 1101 80-81 0.1951703461541996 1101 82-83 0.11540709016292539 1101 84-85 0.3312102950889013 1101 86-87 0.2548896359105264 1101 88-89 0.16320009002528835 1101 90-91 0.364190602584916 1101 92-93 0.3470338861342803 1101 94-95 0.2640096829359919 1101 96-97 0.29878212503377455 1101 98-99 0.3743145136392094 1101 100-101 0.8078669355179606 1102 1 0.06267973911951685 1102 2 0.12677702428097604 1102 3 0.13775108447598683 1102 4 0.1219599468198922 1102 5 0.010847629848420581 1102 6 0.06866930843852259 1102 7 0.06719080683036083 1102 8 0.126760740069507 1102 9 0.1831019761077144 1102 10-11 0.10525737396171309 1102 12-13 0.16818662513120586 1102 14-15 0.30966790712364656 1102 16-17 0.3289882216087321 1102 18-19 0.3387680649262066 1102 20-21 0.22053678408408928 1102 22-23 0.26292924351815117 1102 24-25 0.19882226455198548 1102 26-27 0.18230696768176102 1102 28-29 0.24012776649645673 1102 30-31 0.24242094366235278 1102 32-33 0.26075483716234515 1102 34-35 0.12884367216089032 1102 36-37 0.18042274787041634 1102 38-39 0.18635161214925944 1102 40-41 0.08417098088382602 1102 42-43 0.16137344904034023 1102 44-45 0.3107800970728505 1102 46-47 0.2793544329020463 1102 48-49 0.3234665581463574 1102 50-51 0.30466083127285515 1102 52-53 0.3847395657608885 1102 54-55 0.33005940002607304 1102 56-57 0.45673182413045765 1102 58-59 0.18538229808321915 1102 60-61 0.19441796028576874 1102 62-63 0.27921012380066657 1102 64-65 0.3787724173351634 1102 66-67 0.32234951875104656 1102 68-69 0.223865743143854 1102 70-71 0.27665376062744684 1102 72-73 0.1533813647110378 1102 74-75 0.3348111735872763 1102 76-77 0.17939095988160858 1102 78-79 0.1829845474945273 1102 80-81 0.26693943958314037 1102 82-83 0.3394566660475462 1102 84-85 0.2839459885314355 1102 86-87 0.2586208524080895 1102 88-89 0.2288651243437272 1102 90-91 0.4108664606488759 1102 92-93 0.4086340953604086 1102 94-95 0.4068664948322258 1102 96-97 0.33911736048525043 1102 98-99 0.3422808809505078 1102 100-101 0.6203716976776832 1103 1 0.09838017408632993 1103 2 0.06881148607862286 1103 3 0.1268497816665004 1103 4 0.04157796062910535 1103 5 0.14545161190809353 1103 6 0.15111660287725215 1103 7 0.14897472336373596 1103 8 0.1907882828231564 1103 9 0.19106699157325835 1103 10-11 0.24493426784962935 1103 12-13 0.2649570326518429 1103 14-15 0.3346185084950051 1103 16-17 0.31815898180246904 1103 18-19 0.25087845797948205 1103 20-21 0.28249565813887045 1103 22-23 0.33889261012244276 1103 24-25 0.2945191946929455 1103 26-27 0.28119632620404644 1103 28-29 0.2652697891095528 1103 30-31 0.35505591555156 1103 32-33 0.36914419045725566 1103 34-35 0.3109345596634441 1103 36-37 0.3378015015563349 1103 38-39 0.3421553100392387 1103 40-41 0.32849318788860415 1103 42-43 0.3911346977142074 1103 44-45 0.3613058458435461 1103 46-47 0.38230620321248665 1103 48-49 0.23745170943386285 1103 50-51 0.25872533400826114 1103 52-53 0.27488742062325855 1103 54-55 0.3346983158400576 1103 56-57 0.33195803226234943 1103 58-59 0.380168969190656 1103 60-61 0.3452454526575437 1103 62-63 0.41816340058880996 1103 64-65 0.4221997735962333 1103 66-67 0.4037235847323828 1103 68-69 0.44765484821345325 1103 70-71 0.2787941189461023 1103 72-73 0.3695681340930008 1103 74-75 0.43536171501815346 1103 76-77 0.43261188986524246 1103 78-79 0.32384394672555317 1103 80-81 0.4152684989207387 1103 82-83 0.5326679079756147 1103 84-85 0.4338603094788951 1103 86-87 0.3691009040246094 1103 88-89 0.470326218536421 1103 90-91 0.5335570218640946 1103 92-93 0.5529538878189619 1103 94-95 0.48713136308512617 1103 96-97 0.47708863883562813 1103 98-99 0.3964081618876918 1103 100-101 0.8200708501968528 1104 1 -0.05425471505892432 1104 2 0.04932160838003341 1104 3 0.027272068339854627 1104 4 0.06401845414745821 1104 5 0.08823194736544338 1104 6 0.07590141637030001 1104 7 0.04661459010083746 1104 8 0.0726091110800624 1104 9 0.04684549053281728 1104 10-11 0.16762849276489789 1104 12-13 0.20406904464118014 1104 14-15 0.22545039527187072 1104 16-17 0.26491024956097675 1104 18-19 0.2893277494363531 1104 20-21 0.26712873594383524 1104 22-23 0.2348264386992085 1104 24-25 0.25226536985385906 1104 26-27 0.2971810448173571 1104 28-29 0.2527007407460502 1104 30-31 0.25477594847195206 1104 32-33 0.3535307247235764 1104 34-35 0.2771487506520245 1104 36-37 0.30147119071740747 1104 38-39 0.3093114698540873 1104 40-41 0.32492585382929207 1104 42-43 0.40670395811952886 1104 44-45 0.41074860713118255 1104 46-47 0.40433555123780707 1104 48-49 0.3267112522141957 1104 50-51 0.47654978494573186 1104 52-53 0.45308233377595997 1104 54-55 0.41307795695028915 1104 56-57 0.4922824921846498 1104 58-59 0.4782171062774907 1104 60-61 0.4190927897357497 1104 62-63 0.3660575378122104 1104 64-65 0.2039753183460462 1104 66-67 0.25842210798689536 1104 68-69 0.4212279684986697 1104 70-71 0.4252212142966414 1104 72-73 0.3160660298474909 1104 74-75 0.3427971950053603 1104 76-77 0.3637453791751071 1104 78-79 0.4048417652105698 1104 80-81 0.4162093895682588 1104 82-83 0.42811044643964635 1104 84-85 0.5536301542570925 1104 86-87 0.384073212800633 1104 88-89 0.5160007451261066 1104 90-91 0.6686244260050209 1104 92-93 0.637420207312072 1104 94-95 0.6307785542498117 1104 96-97 0.40284057865887846 1104 98-99 0.2842383990510733 1104 100-101 0.6734989195021797 1105 1 0.016735010613054158 1105 2 0.1180396812804787 1105 3 0.10651123762987424 1105 4 0.13571504436205117 1105 5 0.1260336332316072 1105 6 0.10410219818785293 1105 7 0.13182117212826228 1105 8 0.10412166282647206 1105 9 0.17314361901380693 1105 10-11 0.17939899817881866 1105 12-13 0.17095307503751656 1105 14-15 0.24742661787077935 1105 16-17 0.2135743562940604 1105 18-19 0.17003426804199506 1105 20-21 0.16366187093997553 1105 22-23 0.13547391739525239 1105 24-25 0.2066697240471882 1105 26-27 0.2003025494125552 1105 28-29 0.12117377495921744 1105 30-31 0.15045577227471796 1105 32-33 0.17560611592062259 1105 34-35 0.1786881701697851 1105 36-37 0.2195936754673511 1105 38-39 0.1690997564282739 1105 40-41 0.217787444447481 1105 42-43 0.2549990654176426 1105 44-45 0.2073422810971124 1105 46-47 0.2106702337253239 1105 48-49 0.2637518767457365 1105 50-51 0.26290603083773334 1105 52-53 0.2084587708557919 1105 54-55 0.18799695004308603 1105 56-57 0.21791526855946586 1105 58-59 0.2545296487989077 1105 60-61 0.31340789219075305 1105 62-63 0.390188947735993 1105 64-65 0.16526554793288284 1105 66-67 0.1696108627291153 1105 68-69 0.18493226157647058 1105 70-71 0.11529109356464318 1105 72-73 0.05140464448523119 1105 74-75 0.1490586541674972 1105 76-77 0.10726367347520949 1105 78-79 -0.01034659746974853 1105 80-81 0.16846259236803718 1105 82-83 0.22724963994525638 1105 84-85 0.24555137287772766 1105 86-87 0.3007098982632037 1105 88-89 0.43318228131687775 1105 90-91 0.5165747494560868 1105 92-93 0.5195254644008962 1105 94-95 0.3507846658029514 1105 96-97 0.36744603754427274 1105 98-99 0.3191036566011469 1105 100-101 0.6884906746710548 1106 1 0.015388507534702 1106 2 -0.01036754731499201 1106 3 0.0017541839912169621 1106 4 0.01600471969535988 1106 5 0.0429025706457935 1106 6 -0.029214894428527316 1106 7 0.11702071148247484 1106 8 0.09326991104368432 1106 9 0.040699095826489895 1106 10-11 0.08968490861864353 1106 12-13 0.09889190659175284 1106 14-15 0.18098510659823575 1106 16-17 0.03027055153700786 1106 18-19 0.17222676483076071 1106 20-21 0.10681197945749688 1106 22-23 -0.058260856932598415 1106 24-25 0.07038279487148458 1106 26-27 -0.034341114493187774 1106 28-29 0.028024773763306143 1106 30-31 0.04568653809834444 1106 32-33 0.042287915982633706 1106 34-35 0.1778377471729371 1106 36-37 0.07366972631555768 1106 38-39 0.2084451078921532 1106 40-41 -0.02174172240373906 1106 42-43 0.04960308720973927 1106 44-45 0.21622078577001247 1106 46-47 0.2647529285031922 1106 48-49 0.39681096342687994 1106 50-51 0.30994394552967464 1106 52-53 0.16405738891842958 1106 54-55 0.2567416902654358 1106 56-57 0.4616090719877306 1106 58-59 0.4419737290575725 1106 60-61 0.3142959641223655 1106 62-63 0.2772764704360853 1106 64-65 0.34775104201978024 1106 66-67 0.2578843268740414 1106 68-69 0.3190433037875735 1106 70-71 0.29749422064086417 1106 72-73 0.1755221091616761 1106 74-75 0.19418200979490763 1106 76-77 0.20293404597820341 1106 78-79 0.14355006727756603 1106 80-81 0.1780752512686874 1106 82-83 0.22915644775851973 1106 84-85 0.3157887826310173 1106 86-87 0.28599580870303853 1106 88-89 0.3020798319214748 1106 90-91 0.43495739551180534 1106 92-93 0.4495958900553241 1106 94-95 0.5469688355332103 1106 96-97 0.46473088495535464 1106 98-99 0.36177097289649396 1106 100-101 0.5515471037803259 1107 1 -0.04217741162301536 1107 2 0.03028433923680751 1107 3 0.030450178982057707 1107 4 0.04997656635939762 1107 5 0.04572833999251458 1107 6 0.07454661388528905 1107 7 0.018900306111540033 1107 8 0.08321448561291334 1107 9 0.09358979873226758 1107 10-11 -0.03741035818871552 1107 12-13 0.04986185163013346 1107 14-15 0.17650121243993766 1107 16-17 0.24030162688312373 1107 18-19 0.10645718620317979 1107 20-21 0.012877469589916757 1107 22-23 0.067629080684803 1107 24-25 0.041692597519841 1107 26-27 0.21673028923121507 1107 28-29 0.12013476657423894 1107 30-31 0.06939018597292801 1107 32-33 0.0822411870106734 1107 34-35 0.17658545710545326 1107 36-37 0.17002938866104955 1107 38-39 0.18590852478879327 1107 40-41 0.1554016648089842 1107 42-43 -0.023172938392640674 1107 44-45 0.0802951346951346 1107 46-47 0.22870146865764696 1107 48-49 0.2093529963110896 1107 50-51 0.2967305460259553 1107 52-53 0.30319493211083426 1107 54-55 0.4683392877237509 1107 56-57 0.4197275061613368 1107 58-59 0.35858454513327587 1107 60-61 0.4175345253802192 1107 62-63 0.36872669190590557 1107 64-65 0.4411249201765983 1107 66-67 0.2775348451829842 1107 68-69 0.4126861924033278 1107 70-71 0.49197602764905213 1107 72-73 0.3501957020746431 1107 74-75 0.2932509089391786 1107 76-77 0.13642352906069277 1107 78-79 0.20533260942639942 1107 80-81 0.3523459082571634 1107 82-83 0.5929212027502828 1107 84-85 0.4122301916892468 1107 86-87 0.4399799463621754 1107 88-89 0.5272786909460834 1107 90-91 0.22270060647630174 1107 92-93 0.508841965053584 1107 94-95 0.5629146623925116 1107 96-97 0.3616477004280938 1107 98-99 0.4655591503506322 1107 100-101 0.8088898138287952 1108 1 -0.1652141321685221 1108 2 -0.08857064799889258 1108 3 0.0016481920836142194 1108 4 -0.014602546638755598 1108 5 0.005318765536308945 1108 6 0.018272003794002956 1108 7 0.06909428332564005 1108 8 -0.013324299779270632 1108 9 0.060147769665839235 1108 10-11 0.056901653657639883 1108 12-13 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-0.6911130888238404 2316 72-73 -0.7191713216564182 2316 74-75 -0.7053898121461017 2316 76-77 -0.6158647844569813 2316 78-79 -0.8847301078417402 2316 80-81 -0.8408619682076548 2316 82-83 -0.8730965731441387 2316 84-85 -0.7411538525486634 2316 86-87 -0.8763729385567842 2316 88-89 -0.8387271930374425 2316 90-91 -0.8943426792419302 2316 92-93 -0.838133930377996 2316 94-95 -0.8002783659328507 2316 96-97 -0.541394032642053 2316 98-99 -0.5006592806038412 2316 100-101 -0.6575328732573418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 14.0 5 30.0 6 41.0 7 143.0 8 360.0 9 612.0 10 1048.0 11 1695.0 12 2583.0 13 3875.0 14 5035.0 15 6726.0 16 8938.0 17 12241.0 18 11481.0 19 7357.0 20 6589.0 21 6919.0 22 7520.0 23 8449.0 24 10014.0 25 12293.0 26 15381.0 27 19668.0 28 24630.0 29 31367.0 30 38974.0 31 49036.0 32 62347.0 33 81048.0 34 112419.0 35 170230.0 36 296331.0 37 552128.0 38 610362.0 39 116004.0 40 87.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.79049972266526 18.440771222226644 13.140290290137155 23.62843876497094 2 15.91712354032246 19.787737967443448 39.67514953219684 24.619988960037254 3 20.3546149684762 24.123129180232965 28.129265238029433 27.392990613261397 4 12.852809155786382 15.12222638286891 36.13874744385416 35.88621701749056 5 13.958790417183053 37.845256782859146 33.49835940227927 14.697593397678531 6 33.99505052570183 34.94235566903126 16.666920955317416 14.395672849949499 7 30.426537970453982 31.590279082520734 19.740224526707063 18.242958420318214 8 27.425815645378055 34.80861436951744 18.96986069195865 18.795709293145848 9 27.1278620006007 15.96499709457053 19.474965137025983 37.43217576780278 10-11 26.105199008184915 26.193212134738985 26.375711864465888 21.32587699261021 12-13 26.27871646657774 23.064019738620214 27.44197856999625 23.215285224805797 14-15 23.18848761171082 24.61567903134333 25.268844859656642 26.926988497289212 16-17 22.93993510810303 27.86280023836416 25.945381127643834 23.25188352588897 18-19 22.794652614821135 26.777662936179507 27.864136625368737 22.56354782363062 20-21 23.788868301190877 25.804382382500634 27.736613279842615 22.67013603646587 22-23 23.290847502844947 25.744595474983335 27.75102055139527 23.213536470776454 24-25 23.071668412213526 25.92792072129411 28.066867704258954 22.933543162233413 26-27 22.906096211179662 26.05045570206279 28.049402489087523 22.99404559767003 28-29 22.837254395937812 26.317756903418406 28.01427328399746 22.830715416646324 30-31 23.00344579032271 25.99166462688841 27.790149647432592 23.214739935356285 32-33 22.86779633235729 26.032442687361073 27.841743052277007 23.25801792800463 34-35 23.17004272548581 26.19153799106954 27.722698039667005 22.915721243777647 36-37 23.299009428811374 25.83547499007729 27.895149019422362 22.970366561688977 38-39 23.132442988265588 26.128699057685562 27.87893265829097 22.859925295757876 40-41 23.091114134497897 26.137667945817928 27.86107833192166 22.910139587762522 42-43 23.058999391215057 26.04973988775078 27.58026839285788 23.31099232817628 44-45 23.009060583497394 26.200274468022034 27.63070362510528 23.159961323375295 46-47 23.36774111697811 26.411143840637603 27.20228380546759 23.018831236916697 48-49 23.24522977995874 26.501629366039385 27.14943016331591 23.103710690685965 50-51 23.178520827429868 26.548510146696604 27.08987074133008 23.18309828454345 52-53 23.451336403460683 26.501133805136355 26.933163369734693 23.11436642166827 54-55 23.206846976603067 26.536698727449632 27.00507764684548 23.25137664910182 56-57 23.12133323219615 26.803838330469233 26.82999449848599 23.244833938848622 58-59 23.231310688129195 26.423182878975425 27.16027344082907 23.18523299206631 60-61 23.130269015973738 26.581276948568693 27.264508034820246 23.023946000637327 62-63 23.0442841505662 26.55418680451146 27.306285448746088 23.095243596176253 64-65 23.108428808447904 26.665456244774333 27.054367850264565 23.1717470965132 66-67 23.136002434196275 26.767135386038504 26.8309109796463 23.26595120011892 68-69 23.09975540337954 26.46327497917376 27.193185122283055 23.24378449516365 70-71 23.37435742068089 26.334412608667694 26.964061330156902 23.327168640494513 72-73 23.229617171755482 26.554118589177722 26.843422685643514 23.37284155342328 74-75 23.086219981769684 26.875375166577232 26.743290375988295 23.295114475664793 76-77 23.22470449595934 26.674071556922584 26.812225319483307 23.288998627634772 78-79 23.194771514493908 26.72974630612971 26.792540846452074 23.282941332924302 80-81 23.18495755050815 26.769651539487477 26.571064945946127 23.474325964058245 82-83 23.43395607897363 26.739083715165908 26.775112784281994 23.05184742157847 84-85 23.149333827964643 26.862926605882524 26.56747739557434 23.42026217057849 86-87 23.10420215674952 26.689786232401175 26.869260754395313 23.336750856453992 88-89 23.20337933599012 26.758952626852267 26.566073859171528 23.47159417798608 90-91 22.975404656695012 26.79897586818348 26.80439825696438 23.421221218157132 92-93 23.146161638729147 26.9204922903112 26.599224287301386 23.334121783658265 94-95 23.137606640132873 26.586709271860954 26.835191134864623 23.44049295314155 96-97 23.114161977278638 26.536272726103626 26.84504541460787 23.504519882009863 98-99 22.965484606362434 26.691728585823775 26.63128615946905 23.711500648344742 100-101 22.857675310734155 26.714386133164776 26.692916732509797 23.735021823591275 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 151.0 1 111.5 2 149.5 3 266.5 4 227.5 5 197.0 6 448.0 7 909.0 8 1325.0 9 1434.0 10 1219.5 11 1083.5 12 1132.5 13 1203.0 14 1339.0 15 1496.0 16 1622.0 17 1740.5 18 1898.5 19 2101.0 20 2351.5 21 2702.0 22 3228.0 23 3907.5 24 4623.0 25 5710.0 26 7569.0 27 9898.0 28 12083.0 29 14802.5 30 18566.5 31 23394.0 32 28894.5 33 33308.5 34 37088.0 35 41956.0 36 49452.5 37 56871.0 38 61838.0 39 65544.0 40 67080.5 41 68244.5 42 69773.5 43 72807.0 44 77682.0 45 82724.0 46 85471.5 47 87976.5 48 93701.0 49 92569.0 50 85789.5 51 83114.5 52 83382.0 53 84407.0 54 85152.5 55 81836.0 56 74750.5 57 67437.5 58 63314.5 59 58984.0 60 51300.5 61 42978.0 62 34413.0 63 26107.5 64 18486.5 65 13077.5 66 9421.5 67 7334.5 68 5818.5 69 4542.0 70 3471.5 71 2147.0 72 1477.5 73 1247.0 74 841.0 75 537.0 76 360.5 77 185.5 78 115.5 79 65.0 80 34.5 81 18.0 82 9.5 83 8.0 84 7.5 85 4.5 86 3.0 87 2.5 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2673082296671158 2 0.021578208363539193 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.1796170064181E-4 12-13 4.3592340128362004E-5 14-15 0.0 16-17 5.23108081540344E-4 18-19 0.0 20-21 2.1796170064181002E-5 22-23 2.1796170064181002E-5 24-25 0.0021142284962255573 26-27 0.002092432326161376 28-29 0.0019616553057762902 30-31 9.808276528881451E-4 32-33 0.001220585523594136 34-35 0.0014821395643643083 36-37 6.5388510192543E-5 38-39 6.5388510192543E-5 40-41 5.23108081540344E-4 42-43 0.0029424829586644353 44-45 0.003879718271424218 46-47 0.011573766304080111 48-49 0.012968721188187697 50-51 0.005710596556815422 52-53 0.006822201230088653 54-55 2.1796170064181E-4 56-57 0.0029206867886002546 58-59 0.0012859740337866792 60-61 0.001220585523594136 62-63 0.0 64-65 6.538851019254301E-4 66-67 0.0 68-69 0.0 70-71 1.52573190449267E-4 72-73 0.001220585523594136 74-75 0.0 76-77 0.19629630759801409 78-79 0.10377156567556575 80-81 8.500506325030591E-4 82-83 0.0 84-85 7.19273612117973E-4 86-87 0.12415098468557498 88-89 0.589935138957123 90-91 0.31238270935984214 92-93 0.29616635883209147 94-95 0.0022232093465464624 96-97 0.002637336577765901 98-99 0.0027245212580226255 100-101 8.064582923746971E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2293981.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.938610575318926 #Duplication Level Percentage of deduplicated Percentage of total 1 66.98807111178532 28.09386627548936 2 15.53717261471046 13.032148634597032 3 6.352316535557814 7.992219883077547 4 3.299525150368185 5.535100014590476 5 1.9543061255006384 4.098044177116582 6 1.2249613151915204 3.082390534058861 7 0.8627982318584616 2.5329191335690022 8 0.6078852188939294 2.0395089175748007 9 0.47528826688147785 1.7939636582184497 >10 2.4322386550741633 18.345837487240328 >50 0.172876399102473 4.985509798569454 >100 0.08798716225665353 6.607869880462119 >500 0.003637782924201399 0.9873041903039846 >1k 8.314932398174457E-4 0.641159264246405 >5k 1.0393665497718072E-4 0.2321581508856043 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5326 0.23217280352365605 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3671 0.16002748061121694 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2702 0.11778650302683413 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2600 0.11334008433374122 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.718468025672401E-5 0.0 18-19 0.0 0.0 0.0 2.3975787070599103E-4 0.0 20-21 0.0 0.0 0.0 4.7951574141198207E-4 0.0 22-23 0.0 0.0 0.0 0.0011551970134015932 0.0 24-25 0.0 0.0 0.0 0.0016347127548135753 0.0 26-27 0.0 0.0 0.0 0.0019180629656479283 0.0 28-29 0.0 0.0 0.0 0.0025937442376375392 0.0 30-31 0.0 0.0 0.0 0.004446418693092924 0.0 32-33 0.0 0.0 0.0 0.0082607484543246 0.0 34-35 0.0 0.0 0.0 0.013361052249342954 0.0 36-37 0.0 0.0 0.0 0.021839762404309362 0.0 38-39 0.0 0.0 0.0 0.035484164864486675 0.0 40-41 0.0 0.0 0.0 0.0508286685896701 0.0 42-43 0.0 0.0 0.0 0.06758992336902528 2.1796170064181002E-5 44-45 0.0 0.0 0.0 0.08812191556948379 4.3592340128362004E-5 46-47 0.0 0.0 0.0 0.11386319241528156 4.3592340128362004E-5 48-49 0.0 0.0 0.0 0.14348418753250353 4.3592340128362004E-5 50-51 0.0 0.0 0.0 0.17624383113896758 4.3592340128362004E-5 52-53 0.0 0.0 0.0 0.20769570454158076 4.3592340128362004E-5 54-55 0.0 0.0 0.0 0.24267855749459127 4.3592340128362004E-5 56-57 0.0 0.0 0.0 0.2848759427388457 6.5388510192543E-5 58-59 0.0 0.0 0.0 0.334767376015756 8.718468025672401E-5 60-61 0.0 0.0 0.0 0.3876230884213949 8.718468025672401E-5 62-63 0.0 0.0 0.0 0.4426366216633878 8.718468025672401E-5 64-65 0.0 0.0 0.0 0.4975847663951881 1.08980850320905E-4 66-67 0.0 0.0 0.0 0.5630822574380521 1.30777020385086E-4 68-69 0.0 0.0 0.0 0.640284291805381 1.30777020385086E-4 70-71 0.0 0.0 0.0 0.7277741184430037 1.30777020385086E-4 72-73 0.0 0.0 0.0 0.8168768616653757 1.30777020385086E-4 74-75 0.0 0.0 0.0 0.9090310686967329 1.30777020385086E-4 76-77 0.0 0.0 0.0 1.0101217054544045 1.30777020385086E-4 78-79 0.0 0.0 0.0 1.1313520033513793 1.30777020385086E-4 80-81 0.0 0.0 0.0 1.2720680772857316 1.30777020385086E-4 82-83 0.0 0.0 0.0 1.4184729516068355 2.1796170064181003E-4 84-85 0.0 0.0 0.0 1.5656406918801855 2.1796170064181003E-4 86-87 0.0 0.0 0.0 1.7214397154989514 2.1796170064181003E-4 88-89 0.0 0.0 0.0 1.906598180194169 2.1796170064181003E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1980 0.0 30.913021 2 GTATCAA 10620 0.0 28.446726 1 GTACCTG 2280 0.0 27.543694 1 TACATGG 11775 0.0 27.239725 2 GTACATG 12255 0.0 26.903143 1 ACATGGG 12175 0.0 25.52082 3 TCAACGC 12400 0.0 24.177849 4 ATCAACG 12440 0.0 24.13824 3 CAACGCA 12445 0.0 24.05283 5 GGTATCA 7500 0.0 23.660868 1 AACGCAG 12745 0.0 23.523882 6 TATCAAC 12850 0.0 23.447014 2 CATGGGG 6065 0.0 21.90027 4 AGTACAT 8670 0.0 21.781155 2 GAGTACA 9550 0.0 21.72032 1 GTGTAGC 1230 0.0 21.273586 1 GTGTACG 320 1.8189894E-11 20.814268 1 ACGCAGA 14440 0.0 20.664038 7 CGCAGAG 14765 0.0 20.016417 8 CATGGGA 5855 0.0 19.769022 4 >>END_MODULE