FastQCFastQC Report
Thu 26 May 2016
SRR936332_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936332_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398577
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30790.12836777806174243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30750.037.262161
GTATCAA50400.036.5440671
ATCAACG59900.030.1058623
TCAACGC61300.029.649934
TATCAAC62750.029.191082
CAACGCA62450.028.952355
AACGCAG66050.027.4454156
TACATGG77700.026.8030722
GTACATG81150.026.7223551
GTACCTG22500.025.6729091
ACATGGG79450.025.3190823
ACGCAGA75350.023.9323647
CGCAGAG76950.023.3742088
TACCTGG24000.022.2853832
TAGCGCG1605.130461E-520.7076574
CATGGGG54300.019.874124
AGTACAT55500.019.5296922
GAGTACA66300.019.1389921
GCAGAGT93650.019.054419
CCTACAC13800.018.8641193
Produced by FastQC (version 0.11.5)