##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936332_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2398577 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.60040307232163 33.0 31.0 34.0 30.0 34.0 2 31.700786758148684 33.0 31.0 34.0 30.0 34.0 3 31.773078371050836 34.0 31.0 34.0 30.0 34.0 4 35.25772572654537 37.0 35.0 37.0 33.0 37.0 5 35.23401750287775 37.0 35.0 37.0 33.0 37.0 6 35.31259117385016 37.0 35.0 37.0 33.0 37.0 7 35.2581001151933 37.0 35.0 37.0 33.0 37.0 8 35.25292663108168 37.0 35.0 37.0 33.0 37.0 9 36.91336321493952 39.0 37.0 39.0 33.0 39.0 10-11 36.82465791175351 39.0 37.0 39.0 33.0 39.0 12-13 36.727382735680365 39.0 37.0 39.0 33.0 39.0 14-15 37.96217257148718 40.0 38.0 41.0 33.0 41.0 16-17 37.89532543670684 40.0 38.0 41.0 33.0 41.0 18-19 37.83298263928988 40.0 38.0 41.0 33.0 41.0 20-21 37.74104875515775 40.0 38.0 41.0 32.0 41.0 22-23 37.57903686227293 40.0 38.0 41.0 32.0 41.0 24-25 37.74265637500902 40.0 38.0 41.0 32.0 41.0 26-27 37.69729072696019 40.0 38.0 41.0 32.0 41.0 28-29 37.56749251743847 40.0 38.0 41.0 32.0 41.0 30-31 37.42799209698084 40.0 37.5 41.0 32.0 41.0 32-33 37.266514270753035 40.0 37.0 41.0 31.0 41.0 34-35 37.011269807056436 40.0 37.0 41.0 30.5 41.0 36-37 36.82621320891512 40.0 37.0 41.0 30.0 41.0 38-39 36.668846361822034 40.0 36.5 41.0 30.0 41.0 40-41 36.49433309833289 40.0 36.0 41.0 30.0 41.0 42-43 36.31816364452757 40.0 36.0 41.0 30.0 41.0 44-45 36.151583209544654 40.0 36.0 41.0 29.0 41.0 46-47 35.90752287710589 39.0 35.0 41.0 28.0 41.0 48-49 35.758450948208036 39.0 35.0 41.0 27.5 41.0 50-51 35.47052189694139 38.5 35.0 40.5 27.5 41.0 52-53 35.50107105171108 39.0 35.0 40.5 28.0 41.0 54-55 35.57790452422415 39.0 35.0 41.0 28.0 41.0 56-57 35.416857370015634 39.0 35.0 41.0 27.0 41.0 58-59 35.1777299623902 39.0 35.0 41.0 26.5 41.0 60-61 34.88240465075751 38.0 34.5 40.0 26.0 41.0 62-63 34.567254459623356 37.5 34.0 40.0 26.0 41.0 64-65 34.22935202830678 37.0 34.0 40.0 26.0 41.0 66-67 33.865810228314544 36.5 34.0 39.5 25.0 41.0 68-69 33.47533829433034 36.0 33.5 39.0 24.0 41.0 70-71 33.066024772187845 35.5 33.0 39.0 23.0 40.5 72-73 32.65057115114503 35.0 33.0 38.0 22.0 40.0 74-75 32.22680447615399 35.0 32.5 37.0 21.0 39.5 76-77 31.825221370837795 35.0 32.0 37.0 20.0 39.0 78-79 31.42885281564861 35.0 32.0 36.0 18.5 39.0 80-81 31.032589739666477 35.0 31.5 36.0 17.5 37.5 82-83 30.679096814486257 35.0 31.0 35.0 13.5 37.0 84-85 30.358560513170936 34.5 31.0 35.0 8.5 36.5 86-87 30.035308851873424 34.0 31.0 35.0 7.0 36.0 88-89 29.710524406762843 34.0 30.5 35.0 2.0 36.0 90-91 29.356540982424164 34.0 30.0 35.0 2.0 35.5 92-93 29.037991692574387 34.0 30.0 35.0 2.0 35.0 94-95 28.653177696609283 34.0 29.0 35.0 2.0 35.0 96-97 28.224445785980606 34.0 29.0 35.0 2.0 35.0 98-99 27.71162693547049 34.0 29.0 35.0 2.0 35.0 100-101 24.464451005742156 30.0 21.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29959560095837645 1101 2 0.2886739223012249 1101 3 0.290688186852627 1101 4 0.17063602720632076 1101 5 0.3609788153184823 1101 6 0.21132523855520446 1101 7 0.3811354846445667 1101 8 0.3016477296618021 1101 9 0.3493281372718542 1101 10-11 0.539679952267214 1101 12-13 0.4758010535787278 1101 14-15 0.49881620345271216 1101 16-17 0.6130881659273655 1101 18-19 0.6589425771229784 1101 20-21 0.7342957665628944 1101 22-23 0.6327951112074146 1101 24-25 0.5195002643029198 1101 26-27 0.5348938867627098 1101 28-29 0.44967682835289224 1101 30-31 0.5663892532877668 1101 32-33 0.6365192907648733 1101 34-35 0.5221857793075628 1101 36-37 0.5601265577081165 1101 38-39 0.43970789849471714 1101 40-41 0.3312016124239463 1101 42-43 0.21327873173964917 1101 44-45 0.4337666414036576 1101 46-47 0.22193705423672583 1101 48-49 0.3146657046732315 1101 50-51 0.42530409911559275 1101 52-53 0.4648691316027751 1101 54-55 0.42802655701831327 1101 56-57 0.41331305358097126 1101 58-59 0.4343607456561074 1101 60-61 0.3200814553403788 1101 62-63 0.37356087849200037 1101 64-65 0.3713076431619271 1101 66-67 0.5028076961827779 1101 68-69 0.49049173507209076 1101 70-71 0.5975904188709862 1101 72-73 0.6288040325112974 1101 74-75 0.41719317179283877 1101 76-77 0.18002638003467197 1101 78-79 0.28723986435698023 1101 80-81 0.4187730440749853 1101 82-83 0.6027618824548604 1101 84-85 0.5447671382813795 1101 86-87 0.5195488472765355 1101 88-89 0.3665669579188524 1101 90-91 0.45403569032066926 1101 92-93 0.5351899838634431 1101 94-95 0.7463702399933787 1101 96-97 0.843774872561081 1101 98-99 0.8114402962469818 1101 100-101 1.0953799233402073 1102 1 0.46563564961779846 1102 2 0.5012009327006659 1102 3 0.33747822330067123 1102 4 0.3648869116588003 1102 5 0.4373810882624909 1102 6 0.4573228148879309 1102 7 0.3927181486782274 1102 8 0.41390004226370536 1102 9 0.4513717400230206 1102 10-11 0.5074156376277514 1102 12-13 0.5847243126436368 1102 14-15 0.7368698730291712 1102 16-17 0.6295278173100343 1102 18-19 0.6410451201128353 1102 20-21 0.6321409821460051 1102 22-23 0.485061193588848 1102 24-25 0.6445939429972114 1102 26-27 0.7139560298707224 1102 28-29 0.7409677917024879 1102 30-31 0.6744810921348261 1102 32-33 0.49605416529008295 1102 34-35 0.4931536412934392 1102 36-37 0.5337655708429025 1102 38-39 0.6082374395563406 1102 40-41 0.6924045608314628 1102 42-43 0.7113449150684445 1102 44-45 0.6192378958259184 1102 46-47 0.6828132029782594 1102 48-49 0.5915449190514863 1102 50-51 0.5487081886470762 1102 52-53 0.6224667004923816 1102 54-55 0.5742131189230122 1102 56-57 0.5805566786615088 1102 58-59 0.4833962795955884 1102 60-61 0.5877059590351976 1102 62-63 0.6784942741615012 1102 64-65 0.5527627506569175 1102 66-67 0.6024075119654526 1102 68-69 0.6344443130678954 1102 70-71 0.6381211368765989 1102 72-73 0.5062391552181751 1102 74-75 0.5845137866879284 1102 76-77 0.45120020721929066 1102 78-79 0.4594117679592209 1102 80-81 0.3925394044105808 1102 82-83 0.4984696421442898 1102 84-85 0.45287874792673577 1102 86-87 0.5546586449863824 1102 88-89 0.5926727851872009 1102 90-91 0.3681382007468592 1102 92-93 0.5234729947001675 1102 94-95 0.6242312489553647 1102 96-97 0.7796807356494675 1102 98-99 0.9572176026269013 1102 100-101 1.1610540959322968 1103 1 0.3082770920194342 1103 2 0.2460880998899917 1103 3 0.318383755285339 1103 4 0.23472984073433878 1103 5 0.1728856760452544 1103 6 0.1329532507102158 1103 7 0.24802911358151647 1103 8 0.29704446689493835 1103 9 0.22024518827296902 1103 10-11 0.32448881669134266 1103 12-13 0.38307674181141493 1103 14-15 0.43221650170913506 1103 16-17 0.45079149013245967 1103 18-19 0.36833576622842656 1103 20-21 0.5201936169612296 1103 22-23 0.6133349848122762 1103 24-25 0.5706913556653674 1103 26-27 0.5127245364329909 1103 28-29 0.47881785353335715 1103 30-31 0.4250713891280071 1103 32-33 0.4348125602834898 1103 34-35 0.26893609225574266 1103 36-37 0.33267379469117486 1103 38-39 0.31915998357241193 1103 40-41 0.4014926749555485 1103 42-43 0.21667671800216937 1103 44-45 0.34817542955961756 1103 46-47 0.3296061959343177 1103 48-49 0.4302237196007894 1103 50-51 0.473274518489184 1103 52-53 0.5089151485715178 1103 54-55 0.44452507448676926 1103 56-57 0.4267101832083 1103 58-59 0.47916069370532455 1103 60-61 0.3941838536425877 1103 62-63 0.45838250033269645 1103 64-65 0.458928758021969 1103 66-67 0.38017230867509966 1103 68-69 0.4332063290960946 1103 70-71 0.33001719268153096 1103 72-73 0.3688820323694486 1103 74-75 0.4490894765633797 1103 76-77 0.3200917606432121 1103 78-79 0.27231460808028274 1103 80-81 0.36400872468085765 1103 82-83 0.4723421680551887 1103 84-85 0.5149900844985034 1103 86-87 0.6169821195400722 1103 88-89 0.45069406047466387 1103 90-91 0.7482419809705441 1103 92-93 0.5821692855307496 1103 94-95 0.5004779439070965 1103 96-97 0.7155904694009259 1103 98-99 0.6557486922850906 1103 100-101 0.7559552690109292 1104 1 0.3674869685626412 1104 2 0.3289498394576178 1104 3 0.3087159154998531 1104 4 0.2896503479685748 1104 5 0.3222641465217677 1104 6 0.33985744600656886 1104 7 0.4063516653942685 1104 8 0.41166108049194605 1104 9 0.3658792869266776 1104 10-11 0.3906143962678996 1104 12-13 0.4637317890154762 1104 14-15 0.6292620298919189 1104 16-17 0.5791020873058059 1104 18-19 0.35701070058962614 1104 20-21 0.4793690820190477 1104 22-23 0.5601487940966763 1104 24-25 0.5698364313594553 1104 26-27 0.4849646347515204 1104 28-29 0.5271300148651363 1104 30-31 0.5120547978761181 1104 32-33 0.6185081080182542 1104 34-35 0.46974554424166115 1104 36-37 0.45793428293205807 1104 38-39 0.47722601340314696 1104 40-41 0.45886019632307296 1104 42-43 0.6107208496149283 1104 44-45 0.6286428894780585 1104 46-47 0.5240421492330185 1104 48-49 0.5159438469679785 1104 50-51 0.5293909365663865 1104 52-53 0.6453443983341032 1104 54-55 0.8020692340309168 1104 56-57 0.8192114959841135 1104 58-59 0.7834566548248176 1104 60-61 0.6764050703343898 1104 62-63 0.7674642276105033 1104 64-65 0.7461728225993625 1104 66-67 0.743133707479636 1104 68-69 0.6715022902155923 1104 70-71 0.5768622851344603 1104 72-73 0.7332539668982392 1104 74-75 0.6926898340853072 1104 76-77 0.798727926220554 1104 78-79 0.687600793209608 1104 80-81 0.699521489330877 1104 82-83 0.8704589213091971 1104 84-85 0.8304755881016561 1104 86-87 0.7689472611522739 1104 88-89 0.743613115276073 1104 90-91 0.7267811687254166 1104 92-93 0.6693570404047797 1104 94-95 0.24139699870850606 1104 96-97 0.6896759394863956 1104 98-99 0.7646183643704454 1104 100-101 0.9841907865797772 1105 1 0.27754812785063976 1105 2 0.19878698070388978 1105 3 0.1872190969641352 1105 4 0.10097732732261733 1105 5 0.12298023103863898 1105 6 0.10249540342595509 1105 7 0.2835094499138293 1105 8 0.3001583017446663 1105 9 0.20573989314999608 1105 10-11 0.28029925679357603 1105 12-13 0.17841936116894175 1105 14-15 0.2739457896578088 1105 16-17 0.2483389117480428 1105 18-19 0.1984760535039598 1105 20-21 0.3321652803705959 1105 22-23 0.37327983820760835 1105 24-25 0.321840297442634 1105 26-27 0.33195794964937164 1105 28-29 0.32457163432441405 1105 30-31 0.3976837075869639 1105 32-33 0.39600567829236866 1105 34-35 0.052901771588828694 1105 36-37 0.26200612764437636 1105 38-39 0.3730329240268233 1105 40-41 0.16430246012955507 1105 42-43 0.2560896578410947 1105 44-45 0.26636791431226925 1105 46-47 0.31223835295411817 1105 48-49 0.30417452326405225 1105 50-51 0.1779161869221184 1105 52-53 0.25099478628480654 1105 54-55 0.3159587821824559 1105 56-57 0.39567531857385774 1105 58-59 0.38990699981903987 1105 60-61 0.3595735402782836 1105 62-63 0.15128617300207736 1105 64-65 0.30016825847339135 1105 66-67 0.07151494623711585 1105 68-69 0.14347340198298042 1105 70-71 0.22638498494921322 1105 72-73 0.34074493261360317 1105 74-75 0.40518533707884075 1105 76-77 0.4710840354374035 1105 78-79 0.4367688872842983 1105 80-81 0.31018790541807917 1105 82-83 0.3036337810294434 1105 84-85 0.27859404059447357 1105 86-87 0.3705362213369874 1105 88-89 0.3346092223450974 1105 90-91 0.46429667332981595 1105 92-93 0.31577538282161655 1105 94-95 0.18516900719241036 1105 96-97 0.25733271721346185 1105 98-99 0.2229754938036912 1105 100-101 0.19144592122149717 1106 1 0.20058606766174236 1106 2 0.24855980236169728 1106 3 0.2718680087695944 1106 4 0.20375527642644187 1106 5 0.3024241889171009 1106 6 0.2428759939662939 1106 7 0.19019692241599273 1106 8 0.33864889183269753 1106 9 0.3818824665769114 1106 10-11 0.30291275344860935 1106 12-13 0.2426946930960341 1106 14-15 0.37110410726340604 1106 16-17 0.3631609108352194 1106 18-19 0.33527514150112836 1106 20-21 0.36253018429779615 1106 22-23 0.33535123182852544 1106 24-25 0.3153308669715429 1106 26-27 0.3641034794786009 1106 28-29 0.3317367981189747 1106 30-31 0.225313939370551 1106 32-33 0.313469422273684 1106 34-35 0.16187326019291248 1106 36-37 0.2572554276061396 1106 38-39 0.2877824521948753 1106 40-41 0.15281515127802692 1106 42-43 0.2439572036159845 1106 44-45 0.4112205259380488 1106 46-47 0.21386673852818916 1106 48-49 0.36487760425467286 1106 50-51 0.38903322591455947 1106 52-53 0.25755160554130896 1106 54-55 0.27951968448137166 1106 56-57 0.2672019570334001 1106 58-59 0.2939235334681669 1106 60-61 0.3260849855437158 1106 62-63 0.23464016825703027 1106 64-65 0.18769534512188812 1106 66-67 0.2645226846921389 1106 68-69 0.26598718687695566 1106 70-71 0.41167256600356694 1106 72-73 0.5500721968929483 1106 74-75 0.5735960344032733 1106 76-77 0.5390607296121779 1106 78-79 0.541638419070761 1106 80-81 0.5877869901257888 1106 82-83 0.4941838815635684 1106 84-85 0.6059541254625458 1106 86-87 0.44853867609662856 1106 88-89 0.4386300734106783 1106 90-91 0.738050379112277 1106 92-93 0.48403589573069183 1106 94-95 0.5212968397259949 1106 96-97 0.6548200836305433 1106 98-99 0.7503830702527985 1106 100-101 0.7149655613545569 1107 1 0.12868904110150226 1107 2 0.12695133567551053 1107 3 0.2607877339587503 1107 4 0.2478196422524661 1107 5 0.19235408584182068 1107 6 0.22605422636732442 1107 7 0.18563979816975262 1107 8 0.2540696588078717 1107 9 0.20839581459285483 1107 10-11 0.27617799938224863 1107 12-13 0.27248754529821184 1107 14-15 0.29144672967856167 1107 16-17 0.17287593674195278 1107 18-19 0.30119844822754516 1107 20-21 0.41087001022829384 1107 22-23 0.26336222680843235 1107 24-25 0.33374056258330853 1107 26-27 0.35002462200596796 1107 28-29 0.4220158312736544 1107 30-31 0.4176284866073843 1107 32-33 0.354923169945053 1107 34-35 0.29198543784394815 1107 36-37 0.3414507274750136 1107 38-39 0.35613931588853376 1107 40-41 0.40802270221096393 1107 42-43 0.07233887072822398 1107 44-45 0.36920790568708384 1107 46-47 0.4338404923102388 1107 48-49 0.28169109697490313 1107 50-51 0.29252745259531565 1107 52-53 0.2091409127005619 1107 54-55 0.21252425051702772 1107 56-57 0.15982394784192167 1107 58-59 0.38381873955508183 1107 60-61 0.2940584465223637 1107 62-63 0.36419050285918075 1107 64-65 0.1289387199144869 1107 66-67 0.08669437357367826 1107 68-69 0.21292325389074307 1107 70-71 0.29727501961120595 1107 72-73 0.2504036163953529 1107 74-75 0.19151114687796422 1107 76-77 0.17033425466809504 1107 78-79 0.1946244833262547 1107 80-81 0.13504768699788627 1107 82-83 0.31735958906569905 1107 84-85 0.28433123078265865 1107 86-87 0.07854943343899023 1107 88-89 0.2069030213468359 1107 90-91 0.4283056485126231 1107 92-93 0.19176240462300242 1107 94-95 0.169546066343095 1107 96-97 0.2471455579508337 1107 98-99 0.3143727075585012 1107 100-101 0.3754239472015435 1108 1 0.029336430928236013 1108 2 0.06283472244306765 1108 3 0.06491072890908711 1108 4 0.02498747636832377 1108 5 0.06587957979184722 1108 6 0.004985845753559204 1108 7 0.06662238013177557 1108 8 0.07086917663362868 1108 9 0.0655529112151001 1108 10-11 0.015064744148993725 1108 12-13 -0.06867554811292109 1108 14-15 -0.06442044955146997 1108 16-17 0.1917092979319932 1108 18-19 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4258.0 9 5094.0 10 5872.0 11 6827.0 12 8644.0 13 11916.0 14 11998.0 15 10475.0 16 11508.0 17 13712.0 18 12977.0 19 10786.0 20 10845.0 21 11708.0 22 12456.0 23 13625.0 24 15810.0 25 18269.0 26 21548.0 27 25901.0 28 30806.0 29 38151.0 30 46108.0 31 57278.0 32 71830.0 33 93616.0 34 128980.0 35 190328.0 36 315268.0 37 526036.0 38 517548.0 39 95702.0 40 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.27065045787947 17.424946766688983 12.68431447382965 24.6200883016019 2 15.673750230369398 18.6932363544178 40.62315235402085 25.009861061191952 3 20.587226026440554 22.53526470785674 27.36781493695069 29.509694328752012 4 12.845348325250328 14.874660613863632 35.843553831477685 36.436437229408355 5 14.720892397162435 36.54257807516196 33.572548964015084 15.163980563660518 6 35.03202479597274 34.20928648204218 16.730735715590125 14.027953006394952 7 31.154998659552636 30.352981692782805 19.41680741711428 19.075212230550274 8 26.942879015055365 35.7986413981624 18.714840381796137 18.5436392049861 9 26.734816756983047 16.22379949262708 18.89522132100427 38.1461624293856 10-11 26.202085987304297 25.729671338072492 26.552133943790952 21.516108730832258 12-13 26.531687802848218 22.46774952462615 27.255365141947124 23.74519753057851 14-15 23.264207247335797 24.297721244961686 24.82987411038576 27.608197397316765 16-17 23.64805908590236 27.378102696865962 25.4740401449376 23.49979807229408 18-19 23.53734621063249 26.504161856025593 26.928915924379016 23.029576008962906 20-21 24.11028582506947 25.858319487845506 26.990446092237008 23.040948594848018 22-23 23.665116298462905 25.76159492652255 26.85376018145771 23.719528593556834 24-25 23.559769498067404 25.71875478248976 27.23526534452066 23.486210374922177 26-27 23.561472033301868 26.002408248082908 26.985533000003336 23.450586718611888 28-29 23.338130720352368 26.19179415394296 27.08820291049819 23.381872215206485 30-31 23.61955707740471 25.95832297856939 26.91059988691764 23.51152005710826 32-33 23.359134414620115 25.918880766647995 27.050731470765875 23.671253347966015 34-35 23.647683857645653 26.134704933245274 26.907358485833953 23.31025272327512 36-37 23.789863206480312 25.911293632776832 26.814398921065198 23.48444423967766 38-39 23.500447411973553 26.1263462933075 26.96697402288178 23.406232271837172 40-41 23.763164288646664 25.999292039846843 26.924415828145815 23.31312784336067 42-43 23.684896155123717 25.880167163798994 26.785916990280896 23.6490196907964 44-45 23.625591473977238 26.140709538362284 26.822703384309886 23.41099560335059 46-47 23.7665015473261 26.23409547147622 26.547457024596543 23.451945956601133 48-49 23.87092895146847 26.220903424435267 26.511641353668093 23.396526270428172 50-51 23.6597127300368 26.340371075892133 26.55631800327346 23.44359819079761 52-53 23.83432953260894 26.27010170366416 26.410942466244723 23.48462629748217 54-55 23.798930707105743 26.228111628078448 26.476834081972417 23.49612358284339 56-57 23.62927563285147 26.502975118259066 26.397807075603698 23.46994217328577 58-59 23.73533456001324 26.238787869902115 26.46524540754293 23.56063216254172 60-61 23.735458272803893 26.236539006093995 26.573660770386905 23.45434195071521 62-63 23.68919354497525 26.21510284446755 26.6407896558419 23.454913954715302 64-65 23.582096743110426 26.321021894140618 26.489731622914693 23.607149739834266 66-67 23.570831424117355 26.467429682912385 26.417817990217557 23.543920902752706 68-69 23.55011647833855 26.293709750769246 26.645794619813067 23.51037915107914 70-71 23.547734875222734 26.063888607314983 26.566323500214047 23.82205301724824 72-73 23.54916450894138 26.333918268535943 26.435650076094635 23.681267146428034 74-75 23.59418964959587 26.480092540610112 26.247630762630486 23.678087047163533 76-77 23.683351758442424 26.33070271089977 26.334598584666765 23.65134694599104 78-79 23.63014853501931 26.409511770847843 26.345083410220777 23.615256283912075 80-81 23.555198729165557 26.42096474666048 26.32073823684289 23.703098287331077 82-83 23.783732267878744 26.503804322212737 26.265040556745404 23.44742285316311 84-85 23.72856114104309 26.488042498802933 26.11603972889041 23.667356631263566 86-87 23.608791012678193 26.283806612467302 26.478356248283706 23.629046126570792 88-89 23.741481335769286 26.293157897713808 26.31796155809723 23.64739920841967 90-91 23.55050408639697 26.566556943124038 26.278163324705062 23.60477564577393 92-93 23.60518321587423 26.39614722136611 26.405743130910626 23.592926431849033 94-95 23.617992670236244 26.3553328880262 26.387735998641038 23.638938443096514 96-97 23.667112765023152 26.28188711540769 26.29627310684436 23.754727012724796 98-99 23.408318709590155 26.269219116193376 26.328893620041754 23.99356855417471 100-101 23.57020356111545 26.404374857343882 26.078982801692817 23.94643877984785 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 55.0 1 48.0 2 70.5 3 119.0 4 111.0 5 120.0 6 243.5 7 437.5 8 689.5 9 842.0 10 866.0 11 932.0 12 1062.5 13 1265.5 14 1533.5 15 1781.5 16 1980.5 17 2120.5 18 2204.0 19 2338.5 20 2423.5 21 2540.0 22 2822.5 23 3268.5 24 3954.0 25 4951.5 26 6679.5 27 8925.5 28 11214.5 29 13974.5 30 17390.0 31 21382.5 32 25745.0 33 29952.0 34 34313.0 35 39586.0 36 45614.5 37 51024.5 38 55715.0 39 59903.5 40 64077.0 41 68221.0 42 71720.0 43 75501.5 44 80761.0 45 86793.0 46 91245.0 47 94824.0 48 98476.5 49 99185.0 50 96105.0 51 94314.0 52 94807.0 53 94362.0 54 94039.0 55 90117.0 56 82478.5 57 74974.5 58 69501.5 59 63755.5 60 55509.5 61 46877.5 62 38079.0 63 29632.5 64 21699.0 65 15678.0 66 11236.5 67 8660.5 68 6835.5 69 5297.0 70 4089.5 71 2709.5 72 1949.0 73 1532.5 74 1102.5 75 739.5 76 488.0 77 271.5 78 152.5 79 84.0 80 59.5 81 53.5 82 41.5 83 30.0 84 31.5 85 27.5 86 28.5 87 28.5 88 20.5 89 19.0 90 16.0 91 14.5 92 16.5 93 20.0 94 19.5 95 18.5 96 18.0 97 15.5 98 14.5 99 14.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04636082143704372 2 0.010631303477019917 3 0.009005339415828635 4 0.005378188817786546 5 0.007629523671743705 6 0.005128040500680195 7 0.0051697318868645864 8 0.009547327436225729 9 0.014216762688877614 10-11 0.002022032229943004 12-13 0.008400814316154954 14-15 0.008588425553984718 16-17 0.018406747000408993 18-19 0.010339463773729173 20-21 0.0034186936671201304 22-23 0.009255487732934985 24-25 0.021095841409302263 26-27 0.006378782086211949 28-29 0.016530634622111358 30-31 0.013549700509927345 32-33 0.01219473045893461 34-35 0.2600291756320518 36-37 0.46014782931713255 38-39 0.4567916727292891 40-41 0.4768243837908893 42-43 0.4676939702165075 44-45 0.48078506547840655 46-47 0.4885188176156113 48-49 0.49577311881169545 50-51 0.5049243780791695 52-53 0.4935843210370149 54-55 0.482744560629073 56-57 0.47715791488036446 58-59 0.47421867215436486 60-61 0.47148788635928723 62-63 0.46938247135697536 64-65 0.48516266102776767 66-67 0.4600644465447638 68-69 0.48580887751362584 70-71 0.4670685994237416 72-73 0.4760322474533859 74-75 0.4891650341014693 76-77 0.476782692404705 78-79 0.4765116983945064 80-81 0.4787838789415557 82-83 0.4790131815655699 84-85 0.47936755834813727 86-87 0.480514071468208 88-89 0.4933133270268163 90-91 0.5181613931927138 92-93 0.5062793481301622 94-95 0.4778041313662226 96-97 0.4518303977733464 98-99 0.4428667497437022 100-101 0.4520180090111762 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2398577.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.48743895055268 #Duplication Level Percentage of deduplicated Percentage of total 1 75.06452887741627 41.65138463437634 2 13.730543429644237 15.23745380620594 3 4.723379316563761 7.86264664404405 4 2.15446878261285 4.781838201844083 5 1.1517776889978741 3.1954597101439095 6 0.7103439751835425 2.3649100774133833 7 0.4703243561485234 1.826796579912443 8 0.3325754381099766 1.476300745486451 9 0.2530422451526709 1.263659951683765 >10 1.2848839103395122 12.88468228379284 >50 0.08626167119743527 3.2994459018528577 >100 0.0365148688796469 3.4418886127875346 >500 0.0010542309196100226 0.39318054241368855 >1k 3.0120883417374133E-4 0.32035230804266485 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3079 0.12836777806174243 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 4.169138618439183E-5 0.0 0.0 0.0 8 0.0 4.169138618439183E-5 0.0 0.0 0.0 9 0.0 4.169138618439183E-5 0.0 0.0 0.0 10-11 0.0 4.169138618439183E-5 0.0 0.0 0.0 12-13 0.0 4.169138618439183E-5 0.0 0.0 8.338277236878366E-5 14-15 0.0 4.169138618439183E-5 0.0 0.0 8.338277236878366E-5 16-17 0.0 4.169138618439183E-5 0.0 4.169138618439183E-5 8.338277236878366E-5 18-19 0.0 4.169138618439183E-5 0.0 4.169138618439183E-5 8.338277236878366E-5 20-21 0.0 4.169138618439183E-5 0.0 8.338277236878366E-5 8.338277236878366E-5 22-23 0.0 4.169138618439183E-5 0.0 2.918397032907428E-4 8.338277236878366E-5 24-25 0.0 4.169138618439183E-5 0.0 5.002966342127019E-4 8.338277236878366E-5 26-27 0.0 4.169138618439183E-5 0.0 6.253707927658775E-4 1.0422846546097957E-4 28-29 0.0 4.169138618439183E-5 0.0 9.79747575333208E-4 1.2507415855317547E-4 30-31 0.0 4.169138618439183E-5 0.0 0.0020637236161273956 1.2507415855317547E-4 32-33 0.0 4.169138618439183E-5 0.0 0.004544361094098709 1.2507415855317547E-4 34-35 0.0 4.169138618439183E-5 0.0 0.007275146889176375 1.2507415855317547E-4 36-37 0.0 4.169138618439183E-5 0.0 0.012173884765842413 1.2507415855317547E-4 38-39 0.0 4.169138618439183E-5 0.0 0.020845693092195916 1.6676554473756732E-4 40-41 0.0 4.169138618439183E-5 0.0 0.03158122503467681 1.6676554473756732E-4 42-43 0.0 4.169138618439183E-5 0.0 0.04092009553998058 1.6676554473756732E-4 44-45 0.0 4.169138618439183E-5 0.0 0.05378188817786546 1.6676554473756732E-4 46-47 0.0 4.169138618439183E-5 0.0 0.0688533242835231 1.6676554473756732E-4 48-49 0.0 4.169138618439183E-5 0.0 0.08559241583655641 2.0845693092195913E-4 50-51 0.0 4.169138618439183E-5 0.0 0.10658402878039772 2.5014831710635095E-4 52-53 0.0 4.169138618439183E-5 0.0 0.12580375781140235 2.5014831710635095E-4 54-55 0.0 4.169138618439183E-5 0.0 0.1484004891233427 2.5014831710635095E-4 56-57 0.0 4.169138618439183E-5 0.0 0.1765630204908994 2.7099401019854686E-4 58-59 0.0 1.6676554473756732E-4 0.0 0.20637236161273956 2.918397032907428E-4 60-61 0.0 1.6676554473756732E-4 0.0 0.23970462486716082 2.918397032907428E-4 62-63 0.0 1.6676554473756732E-4 0.0 0.27651811886797883 2.918397032907428E-4 64-65 0.0 1.6676554473756732E-4 0.0 0.3129146990069529 2.918397032907428E-4 66-67 0.0 2.0845693092195913E-4 0.0 0.3571492597485926 2.918397032907428E-4 68-69 0.0 2.0845693092195913E-4 0.0 0.4112646790159332 2.918397032907428E-4 70-71 0.0 2.2930262401415504E-4 0.0 0.47132112081454963 3.126853963829387E-4 72-73 0.0 2.5014831710635095E-4 0.0 0.5329201439019886 4.1691386184391826E-4 74-75 0.0 2.5014831710635095E-4 0.0 0.5963744336746328 5.00296634212702E-4 76-77 0.0 2.5014831710635095E-4 0.0 0.6694385879627796 5.419880203970938E-4 78-79 4.169138618439183E-5 2.5014831710635095E-4 0.0 0.7532382741934072 5.419880203970938E-4 80-81 4.169138618439183E-5 2.5014831710635095E-4 0.0 0.8500873642997494 5.419880203970938E-4 82-83 4.169138618439183E-5 2.5014831710635095E-4 0.0 0.9498965428251834 6.253707927658775E-4 84-85 4.169138618439183E-5 2.5014831710635095E-4 0.0 1.055167292940773 6.253707927658775E-4 86-87 4.169138618439183E-5 2.5014831710635095E-4 0.0 1.1733206813873394 6.253707927658775E-4 88-89 4.169138618439183E-5 2.5014831710635095E-4 0.0 1.302126219004018 6.253707927658775E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3075 0.0 37.26216 1 GTATCAA 5040 0.0 36.544067 1 ATCAACG 5990 0.0 30.105862 3 TCAACGC 6130 0.0 29.64993 4 TATCAAC 6275 0.0 29.19108 2 CAACGCA 6245 0.0 28.95235 5 AACGCAG 6605 0.0 27.445415 6 TACATGG 7770 0.0 26.803072 2 GTACATG 8115 0.0 26.722355 1 GTACCTG 2250 0.0 25.672909 1 ACATGGG 7945 0.0 25.319082 3 ACGCAGA 7535 0.0 23.932364 7 CGCAGAG 7695 0.0 23.374208 8 TACCTGG 2400 0.0 22.285383 2 TAGCGCG 160 5.130461E-5 20.707657 4 CATGGGG 5430 0.0 19.87412 4 AGTACAT 5550 0.0 19.529692 2 GAGTACA 6630 0.0 19.138992 1 GCAGAGT 9365 0.0 19.05441 9 CCTACAC 1380 0.0 18.864119 3 >>END_MODULE