FastQCFastQC Report
Thu 26 May 2016
SRR936332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936332_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398577
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31700.1321616942045221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49850.040.0756761
GGTATCA29550.034.930031
ATCAACG60100.032.2811433
TCAACGC60350.032.147424
CAACGCA61550.031.5983875
TATCAAC63050.030.92512
AACGCAG63600.030.5798856
ACGCAGA76050.025.2618357
TACATGG75950.025.2352712
GTACCTG22900.024.925421
GTACATG80100.024.8222161
ACATGGG75000.024.603023
CGCAGAG77500.024.4831568
TACCTGG21950.024.422962
GTGTAGC18250.021.3721261
CGCGGAA2500.020.06707482-83
GCAGAGT94800.019.7150129
ACGCGAA757.73681E-418.99602978-79
CATGGGG51400.018.7341064
ACCGTAT2803.5472112E-818.6356188
Produced by FastQC (version 0.11.5)