##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936332_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2398577 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.470822908749646 34.0 31.0 34.0 31.0 34.0 2 32.60873759733375 34.0 31.0 34.0 31.0 34.0 3 32.69525055897726 34.0 31.0 34.0 31.0 34.0 4 36.181534718293385 37.0 35.0 37.0 35.0 37.0 5 36.140903960973525 37.0 35.0 37.0 35.0 37.0 6 36.169098594708444 37.0 36.0 37.0 35.0 37.0 7 36.12268357446936 37.0 36.0 37.0 35.0 37.0 8 36.09183486708994 37.0 36.0 37.0 35.0 37.0 9 37.86641746335432 39.0 38.0 39.0 35.0 39.0 10-11 37.79834731176027 39.0 38.0 39.0 35.0 39.0 12-13 37.740612037887466 39.0 38.0 39.0 35.0 39.0 14-15 39.096385690348896 40.0 38.0 41.0 36.0 41.0 16-17 39.02001561759326 40.0 38.0 41.0 36.0 41.0 18-19 39.02462189039585 40.0 38.5 41.0 35.5 41.0 20-21 38.96941895131988 40.0 38.0 41.0 35.0 41.0 22-23 38.85697832506524 40.0 38.0 41.0 35.0 41.0 24-25 39.01808989246541 40.0 38.5 41.0 35.0 41.0 26-27 38.948601399913365 40.0 38.0 41.0 35.0 41.0 28-29 38.862511188925765 40.0 38.0 41.0 35.0 41.0 30-31 38.765320646366575 40.0 38.0 41.0 35.0 41.0 32-33 38.63320627188537 40.0 38.0 41.0 34.0 41.0 34-35 38.5101660276072 40.0 38.0 41.0 34.0 41.0 36-37 38.38082287956567 40.0 38.0 41.0 34.0 41.0 38-39 38.25798713153674 40.0 38.0 41.0 34.0 41.0 40-41 38.12807718910004 40.0 38.0 41.0 33.0 41.0 42-43 38.18256157713511 40.0 38.0 41.0 33.0 41.0 44-45 38.074873977362415 40.0 38.0 41.0 33.0 41.0 46-47 37.9168294367869 40.0 38.0 41.0 33.0 41.0 48-49 37.72277041762678 40.0 37.0 41.0 33.0 41.0 50-51 37.53989073521509 40.0 37.0 41.0 33.0 41.0 52-53 37.330860547733096 40.0 36.5 41.0 32.0 41.0 54-55 37.13381642532218 39.5 36.0 41.0 32.0 41.0 56-57 36.87693119712229 39.0 35.5 41.0 31.0 41.0 58-59 36.60992392572763 39.0 35.0 41.0 31.0 41.0 60-61 36.327620918569636 38.5 35.0 40.5 31.0 41.0 62-63 36.02120152907328 38.0 35.0 40.0 30.0 41.0 64-65 35.63669959313376 37.5 35.0 40.0 29.5 41.0 66-67 35.281746635609366 37.0 34.0 40.0 29.0 41.0 68-69 34.92023666532282 36.0 34.0 39.0 29.0 41.0 70-71 34.538833858575316 36.0 34.0 39.0 29.0 40.5 72-73 34.124408972486606 35.0 34.0 38.5 28.0 40.0 74-75 33.68654310451572 35.0 33.5 37.0 27.5 39.5 76-77 32.960575791396316 35.0 32.5 36.5 26.5 39.0 78-79 33.01357659145402 35.0 33.0 36.0 27.0 39.0 80-81 32.84209179025731 35.0 33.0 36.0 27.0 37.5 82-83 32.55758059883006 35.0 33.0 35.5 26.5 37.0 84-85 32.26057345667869 35.0 33.0 35.0 26.0 37.0 86-87 31.855035923382907 35.0 32.5 35.0 25.5 36.0 88-89 31.36297729862331 35.0 32.0 35.0 24.5 36.0 90-91 31.145663241163405 34.0 32.0 35.0 24.0 36.0 92-93 30.851121727590986 34.0 31.5 35.0 22.5 35.0 94-95 30.680998150153194 34.0 31.0 35.0 20.0 35.0 96-97 30.414606035161682 34.0 31.0 35.0 19.0 35.0 98-99 29.941096741943245 34.0 31.0 35.0 8.5 35.0 100-101 26.5452407823472 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07468822707352274 1101 2 0.03919451708989641 1101 3 0.17391851245498913 1101 4 0.10902762361457974 1101 5 0.08229877453343448 1101 6 0.10709432123135798 1101 7 0.13333367116467798 1101 8 0.04392045118339638 1101 9 0.12024151169113395 1101 10-11 0.12614994244292177 1101 12-13 0.26159034744261334 1101 14-15 0.3976505483588326 1101 16-17 0.362966809475445 1101 18-19 0.3039792524664904 1101 20-21 0.24488214218205684 1101 22-23 0.239572382703102 1101 24-25 0.16823352149523885 1101 26-27 0.32943689177238156 1101 28-29 0.24525314039091484 1101 30-31 0.22021784934793232 1101 32-33 0.3187850141204507 1101 34-35 0.27613024054075197 1101 36-37 0.2442839921476576 1101 38-39 0.27995583724630535 1101 40-41 0.2783093873730138 1101 42-43 0.2480941580840792 1101 44-45 0.30025364940870247 1101 46-47 0.31709744556290786 1101 48-49 0.2698945707149534 1101 50-51 0.218758179681096 1101 52-53 0.25916068735821796 1101 54-55 0.20371583372175905 1101 56-57 0.17898310310784638 1101 58-59 0.10767369388739212 1101 60-61 0.24961232119886034 1101 62-63 0.19450987664138353 1101 64-65 0.25821475905100755 1101 66-67 0.26839436840120356 1101 68-69 0.4358359992020837 1101 70-71 0.36767057511681855 1101 72-73 0.34253811917881904 1101 74-75 0.22743649351847495 1101 76-77 0.18985712780634145 1101 78-79 0.1142257308269663 1101 80-81 0.3194212832670189 1101 82-83 0.28899726174046947 1101 84-85 0.4163694383901273 1101 86-87 0.34004518550615614 1101 88-89 0.4218518078300413 1101 90-91 0.5544900838337945 1101 92-93 0.6389941581425376 1101 94-95 0.6389115628543749 1101 96-97 0.6573483878591198 1101 98-99 0.6602831601224359 1101 100-101 0.8379549207628827 1102 1 0.07225525589469584 1102 2 0.19782330755897704 1102 3 0.1770406704027323 1102 4 0.13590520980944376 1102 5 0.21301073261004433 1102 6 0.21369520928909935 1102 7 0.1912383852207853 1102 8 0.18225114453366587 1102 9 0.16380634944265893 1102 10-11 0.2262400740234014 1102 12-13 0.2680455130870172 1102 14-15 0.38665589112632404 1102 16-17 0.3575030912163015 1102 18-19 0.3227380184895594 1102 20-21 0.32750140080037937 1102 22-23 0.3877147417547562 1102 24-25 0.25309757981212044 1102 26-27 0.34694323587265785 1102 28-29 0.37050940482650674 1102 30-31 0.3617839756903152 1102 32-33 0.3670987976232283 1102 34-35 0.38430453524659924 1102 36-37 0.27577166331043657 1102 38-39 0.3730806002874658 1102 40-41 0.3442905888327914 1102 42-43 0.37545729182539134 1102 44-45 0.36604679568701926 1102 46-47 0.43252936772643125 1102 48-49 0.47230684070497375 1102 50-51 0.3228299174412328 1102 52-53 0.2679504353852096 1102 54-55 0.18440569502379844 1102 56-57 0.31380145599101184 1102 58-59 0.19891976589496352 1102 60-61 0.2623272704809452 1102 62-63 0.31049096180235125 1102 64-65 0.32972492240432416 1102 66-67 0.3571802553075898 1102 68-69 0.25729231620473314 1102 70-71 0.1832626407465625 1102 72-73 0.25073346396991525 1102 74-75 0.39911959320749446 1102 76-77 0.15903561624740092 1102 78-79 0.30413564576601715 1102 80-81 0.3712114709035319 1102 82-83 0.374914754710197 1102 84-85 0.3615323381382254 1102 86-87 0.32931045612185983 1102 88-89 0.4402308582269683 1102 90-91 0.5737464924799127 1102 92-93 0.7530259588901096 1102 94-95 0.5812829421815593 1102 96-97 0.4121207096718926 1102 98-99 0.4688375193508598 1102 100-101 0.7070402538543128 1103 1 -0.02218201480156523 1103 2 -0.0017319200538068458 1103 3 0.09927519047898414 1103 4 -0.045917878728268136 1103 5 0.07685914106276925 1103 6 0.11047112189235264 1103 7 0.14378369939679203 1103 8 0.1384837652746782 1103 9 0.04743995537842238 1103 10-11 0.12651677573894915 1103 12-13 0.11769912353199885 1103 14-15 0.1973604898405128 1103 16-17 0.1148818036309791 1103 18-19 0.12898088744226044 1103 20-21 0.0834487796494372 1103 22-23 0.18070496496081034 1103 24-25 0.10072306916422491 1103 26-27 0.2105432959954996 1103 28-29 0.25147779250952595 1103 30-31 0.21184170728281515 1103 32-33 0.1701373923884404 1103 34-35 0.18792283718623537 1103 36-37 0.26619197663242744 1103 38-39 0.3381181429029567 1103 40-41 0.3217797086480516 1103 42-43 0.2564304620071951 1103 44-45 0.2079988750491779 1103 46-47 0.38542653717036757 1103 48-49 0.34095038740607464 1103 50-51 0.2715068733124255 1103 52-53 0.30756011516275095 1103 54-55 0.16892884573399414 1103 56-57 0.2693921970733584 1103 58-59 0.2335235358704395 1103 60-61 0.23075253027311504 1103 62-63 0.29573422018710005 1103 64-65 0.2480541906136864 1103 66-67 0.2310934324581737 1103 68-69 0.06322363952214971 1103 70-71 0.06398165527852484 1103 72-73 0.0399315072446953 1103 74-75 0.14853578674838985 1103 76-77 0.18458099191267507 1103 78-79 0.11696811483349023 1103 80-81 0.2618989209434943 1103 82-83 0.23655023265263253 1103 84-85 0.2191945844095855 1103 86-87 0.1343016768205132 1103 88-89 0.19811412426371078 1103 90-91 0.2666496058866237 1103 92-93 0.4353477937299317 1103 94-95 0.35635758332335143 1103 96-97 0.4185753749994596 1103 98-99 0.21963278057777913 1103 100-101 0.4426534726809628 1104 1 -0.009927280429188556 1104 2 0.03740801626259582 1104 3 0.005388815416132786 1104 4 0.02476174810821874 1104 5 9.007029866836547E-4 1104 6 0.03064541383135122 1104 7 0.018900396787998375 1104 8 0.13248164248823713 1104 9 0.14308466796430963 1104 10-11 0.09285765055040685 1104 12-13 0.14254008213766411 1104 14-15 0.1938100736626467 1104 16-17 0.2136842169039852 1104 18-19 0.20399066343242822 1104 20-21 0.2708965199991411 1104 22-23 0.2536642409789067 1104 24-25 0.19512494615433695 1104 26-27 0.25853245861210894 1104 28-29 0.3327116900571525 1104 30-31 0.19437475012959027 1104 32-33 0.09320459533800118 1104 34-35 0.08487161099775875 1104 36-37 0.10897070407781939 1104 38-39 0.05500245104215651 1104 40-41 0.14745436369526033 1104 42-43 0.1666882129708327 1104 44-45 0.08759398146781194 1104 46-47 0.06706287033611602 1104 48-49 0.01664370062879783 1104 50-51 -0.021337101884491005 1104 52-53 -0.10140038983696087 1104 54-55 -0.14475441218455387 1104 56-57 -0.10082119882630991 1104 58-59 0.010203162706922342 1104 60-61 -0.02224093377918024 1104 62-63 -0.1482789634339312 1104 64-65 -0.13731020243697856 1104 66-67 -0.12032235436938521 1104 68-69 0.025542280938829265 1104 70-71 -0.04161646773135885 1104 72-73 -0.05752327181792083 1104 74-75 0.1044376916699008 1104 76-77 -0.03651749420541961 1104 78-79 0.017491549278496166 1104 80-81 0.06442179676048454 1104 82-83 0.09451526253923248 1104 84-85 0.21154841939087277 1104 86-87 0.09851965346986802 1104 88-89 0.23548209253364405 1104 90-91 0.4822430364212309 1104 92-93 0.45644985506728375 1104 94-95 0.2517543767985728 1104 96-97 0.1332689675361891 1104 98-99 -0.05220465867142465 1104 100-101 0.24988748195418609 1105 1 -0.06283849475516945 1105 2 -0.003061498060702661 1105 3 0.07536814261027303 1105 4 0.051502371514132506 1105 5 -0.04305709227200083 1105 6 0.021386144628458226 1105 7 0.04745550189713299 1105 8 0.06122672262892337 1105 9 0.06778217380370677 1105 10-11 0.03562630305933823 1105 12-13 0.11232782343225978 1105 14-15 0.036227771732292524 1105 16-17 0.0630233851591413 1105 18-19 0.09811636483119912 1105 20-21 0.06177514109613469 1105 22-23 -0.08060880114528857 1105 24-25 0.023153082648555312 1105 26-27 0.07852096775378925 1105 28-29 0.013972033172031217 1105 30-31 0.06372455011347 1105 32-33 -0.002386244192699394 1105 34-35 0.03225905159700204 1105 36-37 0.08609895601757955 1105 38-39 0.006203567910631591 1105 40-41 0.13043436208438663 1105 42-43 0.12924283481957133 1105 44-45 0.02773670437166942 1105 46-47 0.1110399960106605 1105 48-49 0.13025843470133935 1105 50-51 -0.012560556122416244 1105 52-53 -0.01961544071354382 1105 54-55 -0.08708351251478774 1105 56-57 0.023582914256621734 1105 58-59 -0.032018331641609166 1105 60-61 0.08677971217888825 1105 62-63 0.030813580932061768 1105 64-65 -0.12692343303273645 1105 66-67 -0.05376751773824395 1105 68-69 -0.1152624013174588 1105 70-71 -0.13020174976749388 1105 72-73 -0.10215140294257452 1105 74-75 -0.13158668613234425 1105 76-77 -0.06879800001796355 1105 78-79 -0.15056064284436133 1105 80-81 -0.07050890906816676 1105 82-83 -0.008677048250895325 1105 84-85 2.945734435471081E-4 1105 86-87 -0.05095686812918743 1105 88-89 0.06764232020298522 1105 90-91 0.2257301360534143 1105 92-93 0.27752655923137937 1105 94-95 0.04897681082010763 1105 96-97 0.02398338748947282 1105 98-99 -0.06087209497502144 1105 100-101 -0.03545931107612432 1106 1 0.010362067742512693 1106 2 -0.01432913265102087 1106 3 0.010062901266692847 1106 4 -0.0363839858610433 1106 5 -0.019749005545378395 1106 6 -0.022762111340881575 1106 7 0.013935902882330709 1106 8 0.021579681703926212 1106 9 -0.022618564680101372 1106 10-11 0.09491594413811555 1106 12-13 0.06059201642488432 1106 14-15 0.07156418071087245 1106 16-17 0.017913681662861336 1106 18-19 0.10685234864705251 1106 20-21 0.14266440553173254 1106 22-23 0.056935041190882885 1106 24-25 0.10798610142725806 1106 26-27 0.02849907228461035 1106 28-29 0.0754562581546594 1106 30-31 0.05769589480367188 1106 32-33 0.07446479671584427 1106 34-35 0.20868719128392854 1106 36-37 0.24183556894269032 1106 38-39 0.25648522793670026 1106 40-41 -0.02062936709435803 1106 42-43 -0.008981103404373414 1106 44-45 -0.0429751759248731 1106 46-47 -0.003373801629969364 1106 48-49 0.09166648812217915 1106 50-51 0.0648579166634704 1106 52-53 -0.06687521760591153 1106 54-55 -0.005603378798227254 1106 56-57 0.11186030620812915 1106 58-59 0.14238175306198997 1106 60-61 0.13000177952235958 1106 62-63 0.14768553047174038 1106 64-65 0.28593887016503317 1106 66-67 0.14120546757020946 1106 68-69 0.20536899000083508 1106 70-71 0.1178663091631762 1106 72-73 0.06806284370936311 1106 74-75 0.04459402447329097 1106 76-77 0.09403788470290664 1106 78-79 0.046100452299164374 1106 80-81 0.10409111313568786 1106 82-83 0.011124165559898813 1106 84-85 0.13946998801832677 1106 86-87 0.1605535697390792 1106 88-89 0.28479952761578176 1106 90-91 0.4818821461191618 1106 92-93 0.336722245104383 1106 94-95 0.3814968251292612 1106 96-97 0.43719848528923677 1106 98-99 0.30052136146635533 1106 100-101 0.6447857331465556 1107 1 0.02020344468233759 1107 2 0.08136623299252932 1107 3 0.10781213424908742 1107 4 0.05040315455303812 1107 5 0.03873041122419352 1107 6 0.09208344547358394 1107 7 0.0487243418158414 1107 8 0.1157545914228777 1107 9 0.11202495778725563 1107 10-11 0.023535991411016255 1107 12-13 0.07650566912610657 1107 14-15 0.16937727878303832 1107 16-17 0.15622690067556277 1107 18-19 0.13782458324623548 1107 20-21 0.10299781949208864 1107 22-23 3.904608121061415E-4 1107 24-25 0.16309180769876974 1107 26-27 0.2274287485458899 1107 28-29 0.17701946761942366 1107 30-31 0.04033679052746919 1107 32-33 0.16034000305737806 1107 34-35 0.2105267604810095 1107 36-37 0.22887838189223686 1107 38-39 0.23056045719327756 1107 40-41 0.09947752736486848 1107 42-43 0.039410387474163144 1107 44-45 0.016905665821248306 1107 46-47 0.020689757925822505 1107 48-49 -0.013465634108392521 1107 50-51 0.09553968180119199 1107 52-53 0.17582750631442678 1107 54-55 0.11151734531730995 1107 56-57 -0.09274328466489123 1107 58-59 0.05911918055071652 1107 60-61 0.07489342104612717 1107 62-63 0.031585268646090014 1107 64-65 0.16255480545972034 1107 66-67 0.014830564640895716 1107 68-69 0.14941648780480676 1107 70-71 0.22668011590444337 1107 72-73 0.05092503939539483 1107 74-75 0.05199802527107522 1107 76-77 0.006563717176923944 1107 78-79 -0.017761560915182883 1107 80-81 0.07496481302493407 1107 82-83 0.4399611631792837 1107 84-85 0.2176769278832964 1107 86-87 0.3123608881267046 1107 88-89 0.3943587099728916 1107 90-91 0.031166369108049707 1107 92-93 0.11883471555881897 1107 94-95 0.2079382453530627 1107 96-97 0.1253534207239646 1107 98-99 0.15335052129033855 1107 100-101 0.32206514397519115 1108 1 -0.14917585727928184 1108 2 -0.008325064066305288 1108 3 0.026261680691277434 1108 4 -0.02111807478868144 1108 5 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-0.7613659915287485 2316 82-83 -0.8069207791555328 2316 84-85 -0.7715901713877251 2316 86-87 -0.9674851633930075 2316 88-89 -0.7849592091753834 2316 90-91 -0.8400645877093282 2316 92-93 -0.8269695010144851 2316 94-95 -0.735784360412655 2316 96-97 -0.7021769776286426 2316 98-99 -0.8567091576213492 2316 100-101 -0.9132942679157559 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 21.0 5 42.0 6 60.0 7 150.0 8 341.0 9 662.0 10 1045.0 11 1659.0 12 2463.0 13 3533.0 14 4714.0 15 5988.0 16 7443.0 17 9755.0 18 9506.0 19 7160.0 20 6976.0 21 7530.0 22 8313.0 23 9469.0 24 11249.0 25 13990.0 26 17567.0 27 22064.0 28 27637.0 29 35028.0 30 43437.0 31 54108.0 32 68760.0 33 88634.0 34 122078.0 35 180374.0 36 308755.0 37 574536.0 38 630775.0 39 112694.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41862088539788 17.877459502412528 13.153183355118477 24.550736257071115 2 15.949493243595436 19.129142089867166 39.992077044487395 24.929287622050005 3 20.55089330048608 22.595313804810104 27.70684451656128 29.14694837814254 4 13.1520897598868 15.233865746232036 35.63450329090957 35.979541202971596 5 14.621544357341874 36.576728618676825 33.68359656579714 15.118130458184165 6 35.01434392141674 34.49353512520132 16.4677640117453 14.024356941636645 7 31.060666386778497 30.34999501787935 19.361980040665777 19.22735855467638 8 26.938388886410568 35.9351815680714 18.54474548867933 18.5816840568387 9 26.535858552800264 16.146114967332714 18.971039912414735 38.346986567452284 10-11 26.171265831571695 25.79385337802386 26.496751501259396 21.53812928914505 12-13 26.416048522123127 22.450925048862324 27.296696039650193 23.836330389364356 14-15 23.05731690081244 24.346685555644036 24.87643715419601 27.71956038934752 16-17 23.520690878603034 27.480093338986705 25.516797723976254 23.482418058434007 18-19 23.39289086821061 26.547552986624982 27.047807929451505 23.0117482157129 20-21 23.976819569952994 25.841864440344786 27.076472488873605 23.10484350082862 22-23 23.534917481264408 25.729865192959238 26.975187157883017 23.76003016789333 24-25 23.436290925100728 25.736597613202765 27.337287703124584 23.489823758571923 26-27 23.47304625279156 26.053673392257032 27.029231083847137 23.444049271104266 28-29 23.23878407543317 26.192271444000582 27.153905918252523 23.415038562313725 30-31 23.484718198098815 25.99060142656695 26.987326330368422 23.537354044965813 32-33 23.313694380708007 25.95654165152731 27.07452983563608 23.655234132128605 34-35 23.603755948405322 26.053931930314334 27.045826172733268 23.296485948547073 36-37 23.68877354262427 25.883264021346008 26.970451205403208 23.45751123062652 38-39 23.396874599371085 26.05574774162549 27.093260679008036 23.454116979995394 40-41 23.71207539861963 25.935098101630626 27.051351884892533 23.301474614857216 42-43 23.597758673632438 25.90243738718165 26.850471160923924 23.649332778261993 44-45 23.510163086349543 26.131318783031283 26.90541191852854 23.45310621209064 46-47 23.6206811001444 26.233692912423844 26.69990455608154 23.445721431350222 48-49 23.729157028604693 26.181017449939237 26.656111794360953 23.433713727095117 50-51 23.62895806671043 26.334820480224547 26.598470964778986 23.437750488286035 52-53 23.793240248305498 26.24348189732684 26.456330666504336 23.506947187863325 54-55 23.716214244320575 26.2139941715272 26.540438678045668 23.529352906106553 56-57 23.546949580667533 26.539551876797074 26.44705712640053 23.466441416134863 58-59 23.690303590618054 26.249399374828673 26.541555965758374 23.518741068794906 60-61 23.659296853030305 26.233898835773594 26.651631165637674 23.455173145558422 62-63 23.61470988840467 26.24203850866576 26.678338865085422 23.464912737844145 64-65 23.525855698384753 26.294275401518206 26.583616577081386 23.59625232301565 66-67 23.48269411405179 26.45747874677361 26.47963771853061 23.58018942064399 68-69 23.493825714163023 26.2536912511043 26.70500050655034 23.547482528182336 70-71 23.543306052534977 26.085900640526543 26.57260659304374 23.798186713894736 72-73 23.48461238759552 26.331890300606226 26.471683878898656 23.711813432899604 74-75 23.528096033606595 26.461564502619677 26.311454666662776 23.698884797110953 76-77 23.623731585339492 26.329453118391633 26.367799263361363 23.67901603290751 78-79 23.561246219159873 26.388766874884578 26.395861655942767 23.654125250012783 80-81 23.49315939454961 26.385642128873965 26.38532943930591 23.73586903727051 82-83 23.678164178177312 26.46663000604108 26.36948907623145 23.48571673955016 84-85 23.61822589756792 26.477268700243688 26.194808145703785 23.709697256484606 86-87 23.57012893393769 26.259463218410477 26.486158875629613 23.684248972022225 88-89 23.65975400205795 26.319783906900838 26.366557395746064 23.653904695295143 90-91 23.55306753738474 26.52099312377498 26.31576856440664 23.61017077443364 92-93 23.524422767001425 26.405638420835764 26.412537182758868 23.657401629403946 94-95 23.55611080490402 26.347663269296675 26.35856581678217 23.737660109017135 96-97 23.6273459523677 26.268996353792414 26.317130213033497 23.786527480806388 98-99 23.34060170438437 26.33569868085319 26.31647849507196 24.007221119690474 100-101 23.381977777509096 26.35284239175072 26.271814642195885 23.993365188544296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 40.0 1 37.0 2 58.0 3 88.5 4 79.5 5 74.5 6 158.5 7 347.0 8 557.5 9 644.5 10 598.5 11 566.5 12 603.5 13 713.0 14 808.0 15 892.0 16 1016.5 17 1111.5 18 1219.5 19 1398.5 20 1644.0 21 1969.5 22 2411.0 23 3036.0 24 3826.0 25 4928.0 26 6761.5 27 9020.5 28 11266.0 29 14059.5 30 17424.5 31 21441.0 32 26051.5 33 30343.5 34 34740.5 35 39739.0 36 45839.0 37 51577.0 38 56361.0 39 60756.5 40 64479.0 41 69024.5 42 72498.5 43 75889.5 44 81588.0 45 87679.0 46 92680.0 47 95859.0 48 98739.0 49 99477.5 50 96533.5 51 94330.0 52 94961.5 53 95549.5 54 95281.0 55 90745.5 56 82989.0 57 75329.0 58 69689.0 59 63764.0 60 55152.0 61 46460.0 62 37558.0 63 29055.5 64 21159.0 65 15128.5 66 10753.0 67 8317.0 68 6553.0 69 5040.5 70 3850.5 71 2492.0 72 1778.0 73 1450.5 74 1016.5 75 632.0 76 415.5 77 233.0 78 124.5 79 65.5 80 34.5 81 17.5 82 6.5 83 2.0 84 2.0 85 3.0 86 2.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26157175692087437 2 0.020095248140876862 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.126853963829387E-4 12-13 4.169138618439183E-5 14-15 0.0 16-17 3.3353108947513463E-4 18-19 0.0 20-21 8.338277236878366E-5 22-23 2.0845693092195915E-5 24-25 0.0020428779230351993 26-27 0.0021471063884961794 28-29 0.0023555633194181384 30-31 8.963648029644243E-4 32-33 0.0011465131200707752 34-35 0.001459198516453714 36-37 8.338277236878366E-5 38-39 1.8761123782976322E-4 40-41 7.712906444112488E-4 42-43 0.003606304904949893 44-45 0.00387729891514844 46-47 0.012924329717161467 48-49 0.013195323727360013 50-51 0.0056908742141694845 52-53 0.006691467482594888 54-55 2.918397032907428E-4 56-57 0.002605711636524489 58-59 0.001438352823361518 60-61 0.0013132786648083427 62-63 0.0 64-65 0.0010214389615175996 66-67 0.0 68-69 0.0 70-71 1.459198516453714E-4 72-73 0.001688501140467869 74-75 0.0 76-77 0.19155107382418826 78-79 0.10212305045866779 80-81 0.0013341243579005385 82-83 0.0 84-85 5.00296634212702E-4 86-87 0.11898721617025429 88-89 0.5702964716162958 90-91 0.304096970828954 92-93 0.28531500135288546 94-95 0.0022930262401415504 96-97 0.00239725470560253 98-99 0.0023764090125103342 100-101 6.670621789502693E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2398577.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.64064314779022 #Duplication Level Percentage of deduplicated Percentage of total 1 73.55728446948099 39.45660047147921 2 14.377456759411404 15.424320548087428 3 4.989494014641052 8.029190037821877 4 2.379026612842712 5.10450070314368 5 1.3061702439179979 3.5031905972133717 6 0.7740332435569294 2.4911784601298304 7 0.4846096401119954 1.8196340939858613 8 0.3532813389450154 1.5160190586498474 9 0.25871867352969036 1.2490052438228185 >10 1.3821555854553447 13.42663630786229 >50 0.09558711038845226 3.5046596730804245 >100 0.04070613664520304 3.708075530447773 >500 0.0011653982157077628 0.42729520914750396 >1k 3.1077285752172557E-4 0.3396940651280928 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3170 0.1321616942045221 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.169138618439183E-5 0.0 3 0.0 0.0 0.0 4.169138618439183E-5 0.0 4 0.0 0.0 0.0 4.169138618439183E-5 0.0 5 0.0 0.0 0.0 4.169138618439183E-5 0.0 6 0.0 0.0 0.0 4.169138618439183E-5 0.0 7 0.0 0.0 0.0 4.169138618439183E-5 0.0 8 0.0 0.0 0.0 4.169138618439183E-5 0.0 9 0.0 0.0 0.0 4.169138618439183E-5 0.0 10-11 0.0 0.0 0.0 4.169138618439183E-5 0.0 12-13 0.0 0.0 0.0 4.169138618439183E-5 1.459198516453714E-4 14-15 0.0 0.0 0.0 4.169138618439183E-5 2.2930262401415507E-4 16-17 0.0 0.0 0.0 8.338277236878366E-5 2.918397032907428E-4 18-19 0.0 4.169138618439183E-5 0.0 8.338277236878366E-5 2.918397032907428E-4 20-21 0.0 4.169138618439183E-5 0.0 1.250741585531755E-4 2.918397032907428E-4 22-23 0.0 4.169138618439183E-5 0.0 3.3353108947513463E-4 2.918397032907428E-4 24-25 0.0 4.169138618439183E-5 0.0 5.419880203970938E-4 2.918397032907428E-4 26-27 0.0 4.169138618439183E-5 0.0 6.670621789502693E-4 3.3353108947513463E-4 28-29 2.0845693092195915E-5 4.169138618439183E-5 0.0 0.0010214389615175999 3.3353108947513463E-4 30-31 4.169138618439183E-5 4.169138618439183E-5 0.0 0.0021054150023117873 3.3353108947513463E-4 32-33 4.169138618439183E-5 4.169138618439183E-5 0.0 0.004565206787190905 3.3353108947513463E-4 34-35 4.169138618439183E-5 1.250741585531755E-4 0.0 0.007254301196084178 3.3353108947513463E-4 36-37 4.169138618439183E-5 1.6676554473756732E-4 0.0 0.012382341696764373 3.3353108947513463E-4 38-39 4.169138618439183E-5 1.6676554473756732E-4 0.0 0.02117922418167105 3.5437678256733054E-4 40-41 4.169138618439183E-5 1.6676554473756732E-4 0.0 0.03212321305507391 3.7522247565952645E-4 42-43 4.169138618439183E-5 1.6676554473756732E-4 0.0 0.04164969479820743 3.7522247565952645E-4 44-45 4.169138618439183E-5 1.6676554473756732E-4 0.0 0.05463656159464549 3.7522247565952645E-4 46-47 4.169138618439183E-5 1.8761123782976322E-4 0.0 0.06997899171050168 3.7522247565952645E-4 48-49 4.169138618439183E-5 2.0845693092195913E-4 0.0 0.08684315742208817 3.7522247565952645E-4 50-51 4.169138618439183E-5 2.5014831710635095E-4 0.0 0.10785561605902166 3.7522247565952645E-4 52-53 4.169138618439183E-5 2.5014831710635095E-4 0.0 0.12709619078311848 4.1691386184391826E-4 54-55 4.169138618439183E-5 3.126853963829387E-4 0.0 0.14990137902598083 4.1691386184391826E-4 56-57 4.169138618439183E-5 3.3353108947513463E-4 0.0 0.17823067593827507 4.1691386184391826E-4 58-59 4.169138618439183E-5 3.3353108947513463E-4 0.0 0.20864454215978892 4.1691386184391826E-4 60-61 4.169138618439183E-5 3.7522247565952645E-4 0.0 0.24210187957276336 4.5860524802831013E-4 62-63 4.169138618439183E-5 3.9606816875172236E-4 0.0 0.2791863675837799 5.002966342127019E-4 64-65 4.169138618439183E-5 5.002966342127019E-4 0.0 0.3158956331191369 5.002966342127019E-4 66-67 4.169138618439183E-5 5.836794065814856E-4 0.0 0.36044287925715957 5.002966342127019E-4 68-69 4.169138618439183E-5 5.836794065814856E-4 0.0 0.4147250640692377 5.002966342127019E-4 70-71 4.169138618439183E-5 6.045250996736815E-4 0.0 0.47528180250206686 5.002966342127019E-4 72-73 4.169138618439183E-5 6.253707927658775E-4 0.0 0.5376312705408248 5.211423273048979E-4 74-75 4.169138618439183E-5 6.253707927658775E-4 0.0 0.6013565543236677 5.419880203970938E-4 76-77 4.169138618439183E-5 6.253707927658775E-4 0.0 0.6746083198496442 5.419880203970938E-4 78-79 4.169138618439183E-5 6.253707927658775E-4 0.0 0.7587206914766547 5.419880203970938E-4 80-81 4.169138618439183E-5 6.253707927658775E-4 0.0 0.8555489358899047 5.419880203970938E-4 82-83 4.169138618439183E-5 6.253707927658775E-4 0.0 0.9558167196633671 5.836794065814856E-4 84-85 8.338277236878366E-5 6.253707927658775E-4 0.0 1.0612750810167864 5.836794065814856E-4 86-87 1.2507415855317547E-4 6.253707927658775E-4 0.0 1.1790949383738776 5.836794065814856E-4 88-89 1.2507415855317547E-4 6.253707927658775E-4 0.0 1.308442464010953 5.836794065814856E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4985 0.0 40.075676 1 GGTATCA 2955 0.0 34.93003 1 ATCAACG 6010 0.0 32.281143 3 TCAACGC 6035 0.0 32.14742 4 CAACGCA 6155 0.0 31.598387 5 TATCAAC 6305 0.0 30.9251 2 AACGCAG 6360 0.0 30.579885 6 ACGCAGA 7605 0.0 25.261835 7 TACATGG 7595 0.0 25.235271 2 GTACCTG 2290 0.0 24.92542 1 GTACATG 8010 0.0 24.822216 1 ACATGGG 7500 0.0 24.60302 3 CGCAGAG 7750 0.0 24.483156 8 TACCTGG 2195 0.0 24.42296 2 GTGTAGC 1825 0.0 21.372126 1 CGCGGAA 250 0.0 20.067074 82-83 GCAGAGT 9480 0.0 19.715012 9 ACGCGAA 75 7.73681E-4 18.996029 78-79 CATGGGG 5140 0.0 18.734106 4 ACCGTAT 280 3.5472112E-8 18.635618 8 >>END_MODULE