FastQCFastQC Report
Thu 26 May 2016
SRR936331_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936331_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2766468
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39950.14440795989687935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28830.104212302473768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57800.040.636341
GGTATCA33500.039.4384771
ATCAACG68600.034.299753
TCAACGC69250.033.9082074
CAACGCA70000.033.342025
TATCAAC73100.032.1217652
AACGCAG73900.031.7082546
TACATGG101200.026.4303592
GTACATG106350.026.4045011
ACGCAGA88600.026.180737
CGCAGAG89450.025.7731828
ACATGGG103100.024.4752543
GTACCTG23500.022.7704371
GTGTAGC16050.022.718331
CATGGGG65550.021.8833054
GAGTACA75400.021.5418781
TACCTGG24050.021.4558892
GCAGAGT108350.020.6657739
AGTACAT73100.020.335152
AGAGTAC91650.019.39671910-11
Produced by FastQC (version 0.11.5)