##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936331_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2766468 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.757391735599327 33.0 31.0 34.0 30.0 34.0 2 31.856043879777392 34.0 31.0 34.0 30.0 34.0 3 31.92886236168284 34.0 31.0 34.0 30.0 34.0 4 35.42849908258473 37.0 35.0 37.0 33.0 37.0 5 35.413210635366106 37.0 35.0 37.0 33.0 37.0 6 35.496965806219336 37.0 35.0 37.0 33.0 37.0 7 35.44049163048334 37.0 35.0 37.0 33.0 37.0 8 35.44400188254482 37.0 35.0 37.0 33.0 37.0 9 37.117707849864885 39.0 37.0 39.0 34.0 39.0 10-11 37.035546407910736 39.0 37.0 39.0 33.0 39.0 12-13 36.94270203016988 39.0 37.0 39.0 33.0 39.0 14-15 38.19457915291267 40.0 38.0 41.0 33.0 41.0 16-17 38.13953477864194 40.0 38.0 41.0 33.0 41.0 18-19 38.081712132582055 40.0 38.0 41.0 33.0 41.0 20-21 37.994524606827184 40.0 38.0 41.0 33.0 41.0 22-23 37.83529142574575 40.0 38.0 41.0 33.0 41.0 24-25 37.99857562061083 40.0 38.0 41.0 33.0 41.0 26-27 37.95749905655876 40.0 38.0 41.0 33.0 41.0 28-29 37.83528022012183 40.0 38.0 41.0 33.0 41.0 30-31 37.69969903862976 40.0 38.0 41.0 32.5 41.0 32-33 37.53519451517242 40.0 37.5 41.0 32.0 41.0 34-35 37.27963128436693 40.0 37.0 41.0 31.5 41.0 36-37 37.09524292346776 40.0 37.0 41.0 31.0 41.0 38-39 36.93874337241566 40.0 37.0 41.0 30.5 41.0 40-41 36.76834595592648 40.0 36.5 41.0 30.0 41.0 42-43 36.590426131804165 40.0 36.0 41.0 30.0 41.0 44-45 36.41129176263741 40.0 36.0 41.0 30.0 41.0 46-47 36.172277250270014 39.5 35.5 41.0 29.5 41.0 48-49 36.02697826253548 39.0 35.0 41.0 29.0 41.0 50-51 35.73122967625145 39.0 35.0 40.5 28.5 41.0 52-53 35.757827851252934 39.0 35.0 40.5 28.0 41.0 54-55 35.84791401888617 39.0 35.0 41.0 28.0 41.0 56-57 35.68872764839499 39.0 35.0 41.0 28.0 41.0 58-59 35.451394521823495 39.0 35.0 41.0 28.0 41.0 60-61 35.158567711609166 38.0 35.0 40.5 27.5 41.0 62-63 34.849285081193784 38.0 34.5 40.0 27.0 41.0 64-65 34.519364402552284 37.0 34.0 40.0 26.0 41.0 66-67 34.16316563213455 37.0 34.0 39.5 26.0 41.0 68-69 33.77616567406527 36.0 34.0 39.0 26.0 41.0 70-71 33.3674577114212 35.5 33.5 39.0 25.5 40.5 72-73 32.96306264883599 35.0 33.0 38.5 24.5 40.0 74-75 32.54130736375769 35.0 33.0 37.0 23.5 39.5 76-77 32.15014288977859 35.0 32.5 37.0 22.5 39.0 78-79 31.756005310742793 35.0 32.0 36.0 21.5 39.0 80-81 31.361591928769826 35.0 32.0 36.0 20.0 37.5 82-83 31.015409359515452 35.0 31.5 35.5 19.0 37.0 84-85 30.700243957276932 35.0 31.0 35.0 18.0 37.0 86-87 30.384858960956713 34.0 31.0 35.0 14.5 36.0 88-89 30.065697307903072 34.0 31.0 35.0 8.5 36.0 90-91 29.71511942303327 34.0 30.5 35.0 4.5 35.5 92-93 29.408795258069134 34.0 30.0 35.0 2.0 35.0 94-95 29.03040953302189 34.0 30.0 35.0 2.0 35.0 96-97 28.608074989481175 34.0 29.0 35.0 2.0 35.0 98-99 28.105602703519434 34.0 29.0 35.0 2.0 35.0 100-101 24.82307042770782 30.5 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24024892869329761 1101 2 0.22189156120167652 1101 3 0.286844063314458 1101 4 0.1481855179384226 1101 5 0.41430388466610424 1101 6 0.24768834830932462 1101 7 0.3553007630375049 1101 8 0.30250088913211215 1101 9 0.30142599844398177 1101 10-11 0.4280467889693398 1101 12-13 0.29183148175209084 1101 14-15 0.5739773817333358 1101 16-17 0.4562855198492457 1101 18-19 0.48365522517028126 1101 20-21 0.5355183048491412 1101 22-23 0.5141148630147896 1101 24-25 0.5010466141883043 1101 26-27 0.42181555166764184 1101 28-29 0.31344402269700566 1101 30-31 0.5234002776462248 1101 32-33 0.4847171053879791 1101 34-35 0.22419559337762252 1101 36-37 0.29204793151084374 1101 38-39 0.24642573278593005 1101 40-41 0.18504522474715657 1101 42-43 0.1592475156219706 1101 44-45 0.22985417107184247 1101 46-47 0.0447152188941331 1101 48-49 0.1395161336914157 1101 50-51 0.17931925706454876 1101 52-53 0.19960749962181268 1101 54-55 0.2412304888570631 1101 56-57 0.07922433328001688 1101 58-59 0.12930600081453036 1101 60-61 0.015907530687918836 1101 62-63 0.047399968979213725 1101 64-65 0.11785147704344467 1101 66-67 0.2218974829920839 1101 68-69 0.1748644352464339 1101 70-71 0.3785756281232935 1101 72-73 0.28522431282724625 1101 74-75 0.15473913396426298 1101 76-77 0.24539744858901713 1101 78-79 0.3752145957789388 1101 80-81 0.41419184966707334 1101 82-83 0.4197095697886972 1101 84-85 0.4107444292571856 1101 86-87 0.259496612337589 1101 88-89 0.24705192271959575 1101 90-91 0.2193969362060919 1101 92-93 0.20006432803295127 1101 94-95 0.4026522607925358 1101 96-97 0.4622137075398527 1101 98-99 0.6885261938078742 1101 100-101 0.9284015725662584 1102 1 0.37281607978056996 1102 2 0.3345883289535898 1102 3 0.2629044907914242 1102 4 0.24912381189395916 1102 5 0.35583317339764164 1102 6 0.3370764133532447 1102 7 0.34498755782269086 1102 8 0.32224716693009015 1102 9 0.38366642592795586 1102 10-11 0.46259116213295215 1102 12-13 0.47080893275757063 1102 14-15 0.5543809771478792 1102 16-17 0.4935700310925455 1102 18-19 0.526254713813394 1102 20-21 0.5685981274974239 1102 22-23 0.604237569735524 1102 24-25 0.6776166281168727 1102 26-27 0.6356261666175911 1102 28-29 0.6472910734901802 1102 30-31 0.6981504276070822 1102 32-33 0.5668216828840045 1102 34-35 0.5383951424608355 1102 36-37 0.45159936010932 1102 38-39 0.4765227454534582 1102 40-41 0.47953048651342556 1102 42-43 0.5071731827155332 1102 44-45 0.5493128675920858 1102 46-47 0.6913599345399533 1102 48-49 0.5595538666829114 1102 50-51 0.4963576326703105 1102 52-53 0.6133665967505237 1102 54-55 0.6267041285253967 1102 56-57 0.6749270563448206 1102 58-59 0.6550777076267735 1102 60-61 0.5834515311843376 1102 62-63 0.7814001709136491 1102 64-65 0.6217509852139003 1102 66-67 0.7052436767909498 1102 68-69 0.8028868345430311 1102 70-71 0.8946356384093264 1102 72-73 0.862281493577143 1102 74-75 0.8406662321278873 1102 76-77 0.85761953018946 1102 78-79 0.7540693610985265 1102 80-81 0.8184544191214798 1102 82-83 0.7427115845754848 1102 84-85 0.7671579159531774 1102 86-87 0.8075687520117114 1102 88-89 0.8293246036200728 1102 90-91 0.7636289034786934 1102 92-93 0.78326841397989 1102 94-95 0.8230587169448924 1102 96-97 0.9487752514407823 1102 98-99 1.116581267217704 1102 100-101 1.2582364911666986 1103 1 0.36740670249231755 1103 2 0.3566873460116504 1103 3 0.37055466200284215 1103 4 0.2942000237784228 1103 5 0.2559014533813766 1103 6 0.24694069729568469 1103 7 0.3170171441107712 1103 8 0.2644422362798764 1103 9 0.37996822516093687 1103 10-11 0.42869769425139737 1103 12-13 0.4335216683810543 1103 14-15 0.526381921645033 1103 16-17 0.6035900547731643 1103 18-19 0.4615916086170415 1103 20-21 0.5312923652975954 1103 22-23 0.5686906351317234 1103 24-25 0.6167955293461702 1103 26-27 0.625485267759764 1103 28-29 0.6640865003508907 1103 30-31 0.6090778675509583 1103 32-33 0.6775677248186511 1103 34-35 0.48839339497061474 1103 36-37 0.47116332293977337 1103 38-39 0.5658372263508369 1103 40-41 0.6064397048226908 1103 42-43 0.5178245192045807 1103 44-45 0.6074674667732438 1103 46-47 0.7104498586818266 1103 48-49 0.6695760214491742 1103 50-51 0.6939366341458708 1103 52-53 0.7775847312663515 1103 54-55 0.7833735315060153 1103 56-57 0.74522074551831 1103 58-59 0.7885204082748771 1103 60-61 0.7157332163786663 1103 62-63 0.5953219566611168 1103 64-65 0.5248780550308254 1103 66-67 0.5864156204518594 1103 68-69 0.6093064316655585 1103 70-71 0.6371669930722703 1103 72-73 0.6043329277551521 1103 74-75 0.5512552556729702 1103 76-77 0.5522295031587703 1103 78-79 0.5569063475388525 1103 80-81 0.6371022708592875 1103 82-83 0.605814990817521 1103 84-85 0.6955123512964647 1103 86-87 0.7639912900324113 1103 88-89 0.674648277168437 1103 90-91 0.8280547762221921 1103 92-93 0.852818098536595 1103 94-95 0.8357750754139559 1103 96-97 0.9527969510071514 1103 98-99 0.9300312306056497 1103 100-101 1.2417983195017825 1104 1 0.40936999146814657 1104 2 0.28093825736578637 1104 3 0.39577570860292255 1104 4 0.3096762495373895 1104 5 0.3618778619051284 1104 6 0.3009980755595194 1104 7 0.3796358177323782 1104 8 0.2956831597854048 1104 9 0.3755048126953895 1104 10-11 0.32915534715636596 1104 12-13 0.39289527912169575 1104 14-15 0.5325551727836029 1104 16-17 0.5772832865485498 1104 18-19 0.41374675122297333 1104 20-21 0.5667946720855852 1104 22-23 0.640364069035094 1104 24-25 0.6314372115725391 1104 26-27 0.5472997269241873 1104 28-29 0.5472508997462882 1104 30-31 0.5869386304210593 1104 32-33 0.6609440325747968 1104 34-35 0.5485679600398115 1104 36-37 0.6188819738392723 1104 38-39 0.5810682015244666 1104 40-41 0.7022857575972594 1104 42-43 0.776012134667468 1104 44-45 0.7363707298656124 1104 46-47 0.7462885514690427 1104 48-49 0.6443868351970323 1104 50-51 0.6790692127100328 1104 52-53 0.661965751390241 1104 54-55 0.8036611637635076 1104 56-57 0.7282184337907367 1104 58-59 0.7701903350701684 1104 60-61 0.627393115808637 1104 62-63 0.7808338956473122 1104 64-65 0.9071241848629725 1104 66-67 1.0348055614731706 1104 68-69 0.8872585769289856 1104 70-71 0.7762438333160873 1104 72-73 0.8586787780065777 1104 74-75 0.8030530190271463 1104 76-77 0.8631971192144121 1104 78-79 0.8774861564755518 1104 80-81 0.9068505000166951 1104 82-83 0.9925274968685152 1104 84-85 0.8392738975853504 1104 86-87 0.8415734600925724 1104 88-89 0.8905502954844309 1104 90-91 1.056160295718751 1104 92-93 1.0071592561786318 1104 94-95 0.5863199120617502 1104 96-97 0.8845582601957709 1104 98-99 0.8832071392430159 1104 100-101 0.9436906759820296 1105 1 0.23363401029865116 1105 2 0.2314063288100492 1105 3 0.23148804524887012 1105 4 0.08393660715924511 1105 5 0.10934411776540998 1105 6 0.22874036777653828 1105 7 0.2803915600408686 1105 8 0.22150237869001188 1105 9 0.3157595201648178 1105 10-11 0.3049651627002916 1105 12-13 0.3347968264047978 1105 14-15 0.31276116114409547 1105 16-17 0.39021257890888705 1105 18-19 0.4018302993785028 1105 20-21 0.3872923398643806 1105 22-23 0.3815172433655718 1105 24-25 0.4493251729552412 1105 26-27 0.3855354029929643 1105 28-29 0.3529998422202496 1105 30-31 0.43760074592373854 1105 32-33 0.5469555869737874 1105 34-35 0.2845868368561071 1105 36-37 0.43083267519542545 1105 38-39 0.4988369076408077 1105 40-41 0.5960751116786938 1105 42-43 0.5794234580035038 1105 44-45 0.5730773037065688 1105 46-47 0.5717011571668849 1105 48-49 0.6193109198792257 1105 50-51 0.5525011514670837 1105 52-53 0.6149661588170474 1105 54-55 0.6565784429785424 1105 56-57 0.6799629997122025 1105 58-59 0.6494720716471463 1105 60-61 0.6452436984414902 1105 62-63 0.5040276680878648 1105 64-65 0.6273217899944257 1105 66-67 0.7131855141966383 1105 68-69 0.6950283814079228 1105 70-71 0.7216613925979445 1105 72-73 0.7095156614606069 1105 74-75 0.7228109818430397 1105 76-77 0.7036368741825427 1105 78-79 0.7121874362713818 1105 80-81 0.6589413787255438 1105 82-83 0.662912011395381 1105 84-85 0.8060215958742205 1105 86-87 0.7707469093829573 1105 88-89 0.749338886031758 1105 90-91 0.8990544686391999 1105 92-93 0.8896112239374645 1105 94-95 0.741255377783137 1105 96-97 0.8079732071896082 1105 98-99 0.9139588667389447 1105 100-101 0.940688954275192 1106 1 0.20293402679970995 1106 2 0.21901723498428893 1106 3 0.24206474224404317 1106 4 0.29254765295785035 1106 5 0.2759712947117947 1106 6 0.19365853641255626 1106 7 0.1578293199099221 1106 8 0.2105034842007001 1106 9 0.23624026922840358 1106 10-11 0.2629290102643367 1106 12-13 0.312959741447294 1106 14-15 0.28207402187815944 1106 16-17 0.33348753495139505 1106 18-19 0.33789238749417194 1106 20-21 0.3314812008619725 1106 22-23 0.33069540889479043 1106 24-25 0.2856452941564456 1106 26-27 0.3884200666160993 1106 28-29 0.31810818105897454 1106 30-31 0.4275746887526992 1106 32-33 0.3913019938222675 1106 34-35 0.2570374681577121 1106 36-37 0.2574665825198963 1106 38-39 0.2540912566981035 1106 40-41 0.18158687729834355 1106 42-43 0.23192938297263765 1106 44-45 0.37908941717826394 1106 46-47 0.3434049643184309 1106 48-49 0.30000049199400536 1106 50-51 0.47838358049590113 1106 52-53 0.34842565777711343 1106 54-55 0.40292131207193194 1106 56-57 0.4610491412985738 1106 58-59 0.3682493278651151 1106 60-61 0.42397209217495657 1106 62-63 0.38269764322747335 1106 64-65 0.19350789597959306 1106 66-67 0.33681118126565934 1106 68-69 0.447579623853251 1106 70-71 0.48961438149628833 1106 72-73 0.5484597839093652 1106 74-75 0.5489586971713152 1106 76-77 0.5029944660913443 1106 78-79 0.6253123686502668 1106 80-81 0.7243030360607534 1106 82-83 0.7238656035193571 1106 84-85 0.6330464212077658 1106 86-87 0.6967802203723643 1106 88-89 0.4963890945918763 1106 90-91 0.5707970502635433 1106 92-93 0.4475034309030903 1106 94-95 0.518037492445572 1106 96-97 0.6336138019773294 1106 98-99 0.5788727741117725 1106 100-101 0.7695785355773737 1107 1 0.011987479588434269 1107 2 0.10801658936543745 1107 3 0.21104545954885268 1107 4 0.07456812193793638 1107 5 0.06898938794170562 1107 6 0.1851820060891427 1107 7 0.10556520137048864 1107 8 0.12660992544142857 1107 9 0.15788233559038645 1107 10-11 0.10599830774832952 1107 12-13 0.12166072768575731 1107 14-15 0.20899950855709903 1107 16-17 -0.07984552804892076 1107 18-19 0.20249140411806366 1107 20-21 0.2771001362587455 1107 22-23 0.2613286719415413 1107 24-25 0.23254195061406335 1107 26-27 0.253706126655473 1107 28-29 0.29966932243678457 1107 30-31 0.30273839924830526 1107 32-33 0.3284879702174237 1107 34-35 0.17469196347776972 1107 36-37 0.12515105300112594 1107 38-39 0.12411943493979294 1107 40-41 0.2551104614689095 1107 42-43 -0.13882169756433882 1107 44-45 0.2356883256689457 1107 46-47 0.1838649916061641 1107 48-49 0.11845127707289294 1107 50-51 0.2246114807716424 1107 52-53 0.2068280850495796 1107 54-55 0.34288273396000335 1107 56-57 0.2102514203649335 1107 58-59 0.4059028213628153 1107 60-61 0.46358597711486027 1107 62-63 0.40370641473882785 1107 64-65 0.21547110206558528 1107 66-67 0.30686551820237185 1107 68-69 0.2823125666348787 1107 70-71 0.35843488673280177 1107 72-73 0.3262143183481996 1107 74-75 0.27293214728851467 1107 76-77 0.3512070082712455 1107 78-79 0.22377751318302685 1107 80-81 0.23751126881430196 1107 82-83 0.3131769540077052 1107 84-85 0.3220972183721287 1107 86-87 0.1601058094598109 1107 88-89 0.44724541418275265 1107 90-91 0.4814290093510287 1107 92-93 0.26018006172677843 1107 94-95 0.4134846868658961 1107 96-97 0.6691568692075585 1107 98-99 0.6120353205851998 1107 100-101 0.6769721436326641 1108 1 0.14468784356121134 1108 2 0.1439539173942599 1108 3 0.21301825510452588 1108 4 0.21641714058143435 1108 5 0.22734625366584282 1108 6 0.16414100854667169 1108 7 0.20749989869737817 1108 8 0.1613929156213274 1108 9 0.23332637237095355 1108 10-11 0.16583330778296812 1108 12-13 0.2444161455232745 1108 14-15 0.22860424135767232 1108 16-17 0.36286304820872317 1108 18-19 0.1572529515372736 1108 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8 4120.0 9 4766.0 10 5710.0 11 6977.0 12 9038.0 13 12924.0 14 13121.0 15 11096.0 16 12705.0 17 15225.0 18 14457.0 19 11327.0 20 11295.0 21 12048.0 22 13155.0 23 14438.0 24 16665.0 25 19508.0 26 23109.0 27 28075.0 28 34198.0 29 42207.0 30 51405.0 31 64111.0 32 81713.0 33 107516.0 34 151608.0 35 224330.0 36 374465.0 37 620310.0 38 602802.0 39 112842.0 40 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.115777825192296 17.7600977864249 12.796351814147929 24.327772574234867 2 15.694112909144083 18.860873026287965 40.27498661507188 25.170027449496075 3 20.35234401350292 22.80999646810066 27.82616114354008 29.011498374856348 4 12.829836465819552 14.84604348066556 36.011608146958444 36.312511906556445 5 14.52708064093411 36.900597373724594 33.7076393344841 14.8646826508572 6 34.80852435952169 34.37097729808906 16.646742562621757 14.173755779767497 7 30.70862270652317 30.87501680931084 19.59354724898457 18.822813235181417 8 27.248121888114746 35.493955807388375 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23.268030706532596 25.90408628109792 27.214585476451415 23.613297535918072 34-35 23.510432935709545 26.18806483534664 27.083266513038883 23.21823571590493 36-37 23.672787143367604 25.856266359117676 27.06177536916047 23.409171128354256 38-39 23.393330337568255 26.24591600056284 27.05341086010231 23.30734280176659 40-41 23.62191812166706 26.028691103469754 27.073966895998154 23.27542387886503 42-43 23.55128267052006 25.894404797226606 26.9494089717754 23.604903560477936 44-45 23.442577429134264 26.148862764840157 27.05217466633178 23.3563851396938 46-47 23.711547294741063 26.23898746923066 26.74686204057584 23.30260319545243 48-49 23.706018675346883 26.276825899406543 26.61028958612105 23.40686583912552 50-51 23.540667909544236 26.455272120613984 26.674764807854434 23.32929516198735 52-53 23.813720390234263 26.312369042810108 26.464268485125007 23.409642081830626 54-55 23.665346546521025 26.282068395263114 26.577365596641094 23.47521946157477 56-57 23.478279029393498 26.565704074702268 26.530274249726695 23.42574264617754 58-59 23.582104459357495 26.348843734081996 26.652254012586408 23.416797793974105 60-61 23.587975918132983 26.34169390228445 26.70783575896487 23.362494420617693 62-63 23.46000062829138 26.39982320679557 26.77705225659202 23.36312390832103 64-65 23.447800154627462 26.39767227005067 26.639002988846812 23.515524586475056 66-67 23.496208827591666 26.599103830426806 26.371257645938034 23.533429696043495 68-69 23.501274126078094 26.266121358987903 26.73990265753042 23.49270185740358 70-71 23.4580912451683 26.154945989000783 26.621178877739887 23.765783888091033 72-73 23.4790311605687 26.433211644567105 26.431159588070543 23.65659760679365 74-75 23.381449605232497 26.536193271979748 26.468574886680628 23.613782236107127 76-77 23.577708818205327 26.43734582160197 26.406128782001552 23.578816578191148 78-79 23.601573659794134 26.471919245334824 26.361035213034995 23.565471881836046 80-81 23.51423075058145 26.44758703864221 26.41186556011805 23.626316650658286 82-83 23.676167417777354 26.5241408717533 26.424840261857234 23.374851448612116 84-85 23.594853686813362 26.611119424452067 26.204112779267724 23.589914109466843 86-87 23.492412118817114 26.39978628551587 26.525385314429794 23.58241628123722 88-89 23.552253358068935 26.43363397965815 26.32006064993805 23.694052012334865 90-91 23.34512592970479 26.676702455043795 26.38113358989634 23.597038025355083 92-93 23.482231689676603 26.50295434592305 26.466060217539116 23.54875374686123 94-95 23.469178913701466 26.428842922392175 26.508511376680698 23.593466787225662 96-97 23.56046236007849 26.283458071112882 26.476338068672835 23.6797415001358 98-99 23.313670157871186 26.34754170974321 26.373482941688504 23.9653051906971 100-101 23.42086008972355 26.435821881870652 26.195445558575386 23.94787246983041 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 84.0 1 69.5 2 107.5 3 202.5 4 185.0 5 150.0 6 318.5 7 635.0 8 951.5 9 1141.5 10 1151.0 11 1152.0 12 1296.5 13 1550.5 14 1825.0 15 2123.0 16 2392.0 17 2555.5 18 2678.5 19 2756.5 20 2903.5 21 3162.5 22 3494.5 23 4113.0 24 5062.5 25 6326.5 26 8439.0 27 11426.0 28 14376.0 29 17534.0 30 21259.0 31 26224.0 32 32408.0 33 37345.5 34 42135.5 35 47568.5 36 53595.5 37 60062.0 38 65391.0 39 69773.5 40 74876.5 41 80082.0 42 83159.5 43 87216.5 44 92969.0 45 98630.0 46 103212.5 47 106230.5 48 108960.5 49 109715.5 50 107911.0 51 107618.0 52 109491.0 53 108886.0 54 107598.0 55 102034.0 56 92452.0 57 84095.0 58 79011.5 59 73361.5 60 63728.0 61 53907.0 62 43833.5 63 33973.5 64 24915.0 65 18176.5 66 13263.0 67 10276.0 68 8268.0 69 6555.5 70 4882.0 71 3204.5 72 2254.0 73 1780.0 74 1319.0 75 885.5 76 583.5 77 331.5 78 209.5 79 124.5 80 82.0 81 60.0 82 41.0 83 34.5 84 27.5 85 26.0 86 30.5 87 32.0 88 31.0 89 23.5 90 17.0 91 20.0 92 26.0 93 24.5 94 24.5 95 22.5 96 19.5 97 22.5 98 19.5 99 11.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0454731448185918 2 0.010735710660669128 3 0.00907294065935337 4 0.004879868482122331 5 0.0069764045707378505 6 0.0049521628300056245 7 0.005205193047597153 8 0.009362118050886546 9 0.014567311098483698 10-11 0.0021869040234696368 12-13 0.008512659463257842 14-15 0.00833192359354961 16-17 0.017585600122611216 18-19 0.010446533269135952 20-21 0.0034701286983981018 22-23 0.009145235007236664 24-25 0.021362979799513314 26-27 0.006018504461284208 28-29 0.017278349144107218 30-31 0.013699778923884173 32-33 0.01252499577078065 34-35 0.2603138731407701 36-37 0.4573340447097165 38-39 0.45474952177288874 40-41 0.4762570902681686 42-43 0.46546715884658707 44-45 0.4815345776636491 46-47 0.4888724539738034 48-49 0.49501747354388337 50-51 0.505500153986961 52-53 0.4937703960428965 54-55 0.4811008115763494 56-57 0.47490157124535687 58-59 0.47354605222254514 60-61 0.4708892349378341 62-63 0.4687746252622477 64-65 0.4843540572310976 66-67 0.456773763513621 68-69 0.48453479310080577 70-71 0.46660579482574893 72-73 0.4747027617886778 74-75 0.4886555709301536 76-77 0.4758413977678397 78-79 0.47501001276718186 80-81 0.47795600744342603 82-83 0.47757646211703875 84-85 0.47699810733397247 86-87 0.47938382081412106 88-89 0.49236065625917236 90-91 0.5175371629095293 92-93 0.5059700672482025 94-95 0.4765101204857602 96-97 0.449092489051021 98-99 0.43974844458710527 100-101 0.4501407570953288 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2766468.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.220344850685606 #Duplication Level Percentage of deduplicated Percentage of total 1 69.36966222335099 32.75659372362211 2 14.777000363093737 13.955501060079852 3 6.006672201840612 8.50911398327822 4 3.0872571135742426 5.831253821828319 5 1.7796190301047319 4.201711215219404 6 1.1265775705158556 3.1918428828483765 7 0.7767879860455702 2.5676137603858966 8 0.5589742393777742 2.1115965076854546 9 0.3992130237909822 1.6965878987065623 >10 1.9470526872656688 16.161181071556406 >50 0.11752856062900179 3.7966566167480695 >100 0.05150689570516211 4.213902388799808 >500 0.0016877965544366798 0.5588664632977952 >1k 4.603081511968597E-4 0.44757860594389404 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3995 0.14440795989687935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2883 0.104212302473768 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.614717394164689E-5 0.0 0.0 0.0 0.0 7 3.614717394164689E-5 0.0 0.0 0.0 0.0 8 3.614717394164689E-5 0.0 0.0 0.0 0.0 9 3.614717394164689E-5 0.0 0.0 3.614717394164689E-5 0.0 10-11 3.614717394164689E-5 0.0 0.0 3.614717394164689E-5 0.0 12-13 3.614717394164689E-5 0.0 0.0 3.614717394164689E-5 0.0 14-15 3.614717394164689E-5 0.0 0.0 5.422076091247034E-5 0.0 16-17 3.614717394164689E-5 0.0 0.0 9.036793485411724E-5 0.0 18-19 3.614717394164689E-5 0.0 0.0 1.0844152182494068E-4 0.0 20-21 3.614717394164689E-5 0.0 0.0 1.2651510879576412E-4 0.0 22-23 3.614717394164689E-5 0.0 0.0 3.433981524456455E-4 0.0 24-25 3.614717394164689E-5 0.0 0.0 5.2413402215388E-4 0.0 26-27 3.614717394164689E-5 0.0 0.0 6.325755439788207E-4 3.614717394164689E-5 28-29 5.422076091247034E-5 0.0 0.0 9.940472833952895E-4 3.614717394164689E-5 30-31 7.229434788329378E-5 0.0 0.0 0.0025303021759152827 3.614717394164689E-5 32-33 7.229434788329378E-5 0.0 0.0 0.0049702364169764476 3.614717394164689E-5 34-35 7.229434788329378E-5 0.0 0.0 0.007898157506249847 3.614717394164689E-5 36-37 7.229434788329378E-5 0.0 0.0 0.014043177076329818 3.614717394164689E-5 38-39 7.229434788329378E-5 0.0 0.0 0.02421860654090342 3.614717394164689E-5 40-41 7.229434788329378E-5 0.0 0.0 0.03658094002894666 3.614717394164689E-5 42-43 7.229434788329378E-5 0.0 0.0 0.04890712634304825 3.614717394164689E-5 44-45 1.0844152182494068E-4 0.0 0.0 0.06426967526824817 3.614717394164689E-5 46-47 1.0844152182494068E-4 0.0 0.0 0.08337345669640855 3.614717394164689E-5 48-49 1.0844152182494068E-4 0.0 0.0 0.10410386095194306 3.614717394164689E-5 50-51 1.0844152182494068E-4 0.0 0.0 0.12796099575343 3.614717394164689E-5 52-53 1.0844152182494068E-4 0.0 0.0 0.15120362859790895 3.614717394164689E-5 54-55 1.0844152182494068E-4 0.0 0.0 0.17654279753100344 3.614717394164689E-5 56-57 1.0844152182494068E-4 0.0 0.0 0.20820772190388612 3.614717394164689E-5 58-59 1.0844152182494068E-4 0.0 0.0 0.2453489431289283 3.614717394164689E-5 60-61 1.0844152182494068E-4 0.0 0.0 0.2858518515305436 3.614717394164689E-5 62-63 1.0844152182494068E-4 0.0 0.0 0.32819826580318295 3.614717394164689E-5 64-65 1.0844152182494068E-4 0.0 0.0 0.37027357627125995 3.614717394164689E-5 66-67 1.0844152182494068E-4 0.0 0.0 0.42060851598500326 3.614717394164689E-5 68-69 1.0844152182494068E-4 0.0 0.0 0.48178760788124064 3.614717394164689E-5 70-71 1.0844152182494068E-4 0.0 0.0 0.5526360688068686 3.614717394164689E-5 72-73 1.0844152182494068E-4 0.0 0.0 0.6258702432126452 3.614717394164689E-5 74-75 1.0844152182494068E-4 0.0 0.0 0.699990023379992 3.614717394164689E-5 76-77 1.0844152182494068E-4 0.0 0.0 0.7835080687721673 3.614717394164689E-5 78-79 1.0844152182494068E-4 0.0 0.0 0.8766954831937329 3.614717394164689E-5 80-81 1.0844152182494068E-4 0.0 0.0 0.9873962033900265 3.614717394164689E-5 82-83 1.0844152182494068E-4 3.614717394164689E-5 0.0 1.1020550391329305 3.614717394164689E-5 84-85 1.2651510879576412E-4 3.614717394164689E-5 0.0 1.2205454753136489 3.614717394164689E-5 86-87 1.4458869576658757E-4 3.614717394164689E-5 0.0 1.3497896957420075 5.422076091247034E-5 88-89 1.4458869576658757E-4 3.614717394164689E-5 0.0 1.4997823940128714 7.229434788329378E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5780 0.0 40.63634 1 GGTATCA 3350 0.0 39.438477 1 ATCAACG 6860 0.0 34.29975 3 TCAACGC 6925 0.0 33.908207 4 CAACGCA 7000 0.0 33.34202 5 TATCAAC 7310 0.0 32.121765 2 AACGCAG 7390 0.0 31.708254 6 TACATGG 10120 0.0 26.430359 2 GTACATG 10635 0.0 26.404501 1 ACGCAGA 8860 0.0 26.18073 7 CGCAGAG 8945 0.0 25.773182 8 ACATGGG 10310 0.0 24.475254 3 GTACCTG 2350 0.0 22.770437 1 GTGTAGC 1605 0.0 22.71833 1 CATGGGG 6555 0.0 21.883305 4 GAGTACA 7540 0.0 21.541878 1 TACCTGG 2405 0.0 21.455889 2 GCAGAGT 10835 0.0 20.665773 9 AGTACAT 7310 0.0 20.33515 2 AGAGTAC 9165 0.0 19.396719 10-11 >>END_MODULE