FastQCFastQC Report
Thu 26 May 2016
SRR936331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936331_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2766468
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38590.13949194424081537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28220.10200732486332754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34050.044.5665971
GTATCAA59100.039.7626531
ATCAACG72300.032.413793
TCAACGC72650.032.322934
CAACGCA75150.031.2476525
TATCAAC75850.031.0291082
AACGCAG78550.030.0762926
TACATGG99400.026.2554532
GTACATG105100.025.5729981
ACATGGG99300.025.3680133
ACGCAGA92700.025.2806597
CGCAGAG93700.025.0614838
GTACCGT2503.8926373E-1022.7710176
GTATAGG7650.021.764191
TAGATCG1100.002899701721.5634635
CATGGGG61050.021.4469034
TAGCGCG1553.947509E-521.4243434
GTGTACG3001.6370905E-1020.6137981
GTACCTG25700.020.5459271
GCAGAGT114700.020.142199
Produced by FastQC (version 0.11.5)