##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936331_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2766468 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4175638395239 34.0 31.0 34.0 31.0 34.0 2 32.55795982458499 34.0 31.0 34.0 31.0 34.0 3 32.6440605855553 34.0 31.0 34.0 31.0 34.0 4 36.147896885125725 37.0 35.0 37.0 35.0 37.0 5 36.10396397138879 37.0 35.0 37.0 35.0 37.0 6 36.1359455450054 37.0 36.0 37.0 35.0 37.0 7 36.08490248215414 37.0 36.0 37.0 35.0 37.0 8 36.057687636365216 37.0 35.0 37.0 35.0 37.0 9 37.82025817757516 39.0 38.0 39.0 35.0 39.0 10-11 37.74758338068613 39.0 37.5 39.0 35.0 39.0 12-13 37.689575841831534 39.0 37.0 39.0 35.0 39.0 14-15 39.02164926541713 40.0 38.0 41.0 35.5 41.0 16-17 38.937774085946415 40.0 38.0 41.0 35.0 41.0 18-19 38.94866197620938 40.0 38.0 41.0 35.0 41.0 20-21 38.89385147415405 40.0 38.0 41.0 35.0 41.0 22-23 38.77602198904886 40.0 38.0 41.0 34.5 41.0 24-25 38.94010792823195 40.0 38.0 41.0 35.0 41.0 26-27 38.86611918157015 40.0 38.0 41.0 35.0 41.0 28-29 38.78000052774874 40.0 38.0 41.0 35.0 41.0 30-31 38.6778477105103 40.0 38.0 41.0 34.0 41.0 32-33 38.54021083923617 40.0 38.0 41.0 34.0 41.0 34-35 38.40658829236413 40.0 38.0 41.0 34.0 41.0 36-37 38.270691907515285 40.0 38.0 41.0 34.0 41.0 38-39 38.13919770624493 40.0 38.0 41.0 33.0 41.0 40-41 38.007625427078864 40.0 38.0 41.0 33.0 41.0 42-43 38.05827177469611 40.0 38.0 41.0 33.0 41.0 44-45 37.94742917684209 40.0 38.0 41.0 33.0 41.0 46-47 37.785383203420395 40.0 37.0 41.0 33.0 41.0 48-49 37.583454607101906 40.0 37.0 41.0 33.0 41.0 50-51 37.39428162552395 40.0 37.0 41.0 32.0 41.0 52-53 37.17712042937059 40.0 36.0 41.0 32.0 41.0 54-55 36.98301462370069 39.0 36.0 41.0 31.0 41.0 56-57 36.72092267107373 39.0 35.0 41.0 31.0 41.0 58-59 36.44999978311696 39.0 35.0 41.0 31.0 41.0 60-61 36.16051965900202 38.5 35.0 40.0 30.0 41.0 62-63 35.855826273790264 38.0 35.0 40.0 30.0 41.0 64-65 35.467454891941635 37.0 34.5 40.0 29.0 41.0 66-67 35.11345585779412 37.0 34.0 39.5 29.0 41.0 68-69 34.75052919462651 36.0 34.0 39.0 29.0 41.0 70-71 34.36804474152602 36.0 34.0 39.0 28.0 40.5 72-73 33.95782365818076 35.0 34.0 38.0 28.0 40.0 74-75 33.52123375365267 35.0 33.0 37.0 27.0 39.0 76-77 32.799747367401324 35.0 32.5 36.0 26.0 39.0 78-79 32.8699947008243 35.0 33.0 36.0 26.0 39.0 80-81 32.70779492117747 35.0 33.0 36.0 26.0 37.5 82-83 32.421132830743026 35.0 33.0 35.5 26.0 37.0 84-85 32.12597362413012 35.0 33.0 35.0 26.0 37.0 86-87 31.709583121872367 35.0 32.0 35.0 25.0 36.0 88-89 31.2142439384804 34.5 32.0 35.0 23.5 36.0 90-91 30.984847285419534 34.0 31.0 35.0 23.0 35.5 92-93 30.680882807970306 34.0 31.0 35.0 20.5 35.0 94-95 30.499099935368854 34.0 31.0 35.0 20.0 35.0 96-97 30.219543475651985 34.0 31.0 35.0 18.5 35.0 98-99 29.72364274591284 34.0 31.0 35.0 2.0 35.0 100-101 26.26811895167412 30.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13354616280073373 1101 2 0.08915054600853978 1101 3 0.15682937574757005 1101 4 0.1592614158936172 1101 5 0.10305502745563189 1101 6 0.10981908205484103 1101 7 0.13218195648560993 1101 8 0.08441774804447988 1101 9 0.21913651427694703 1101 10-11 0.16151822554590467 1101 12-13 0.2687738498149628 1101 14-15 0.2943751926456244 1101 16-17 0.30320916516737384 1101 18-19 0.27271927306975385 1101 20-21 0.14819436514584083 1101 22-23 0.2364996047365011 1101 24-25 0.16793995564243858 1101 26-27 0.19979854149506338 1101 28-29 0.23858661179153273 1101 30-31 0.1684886899736071 1101 32-33 0.2649099921257303 1101 34-35 0.23369922226942208 1101 36-37 0.19535891924873283 1101 38-39 0.3077071428017035 1101 40-41 0.34643880379741887 1101 42-43 0.2357710251057128 1101 44-45 0.20732674602432155 1101 46-47 0.331967798088165 1101 48-49 0.34636523001208985 1101 50-51 0.2870152478878083 1101 52-53 0.40974783482535315 1101 54-55 0.4103298516269618 1101 56-57 0.2831218698700866 1101 58-59 0.17771635711574874 1101 60-61 0.3758800198553587 1101 62-63 0.4017802086811031 1101 64-65 0.4943969234841603 1101 66-67 0.4574304194255845 1101 68-69 0.5622506490411041 1101 70-71 0.5150621510643916 1101 72-73 0.4565197403160184 1101 74-75 0.37681576693926644 1101 76-77 0.1782798702962154 1101 78-79 0.1546516750873863 1101 80-81 0.30230119208042083 1101 82-83 0.20106893312206608 1101 84-85 0.4121867724180035 1101 86-87 0.22694459715590298 1101 88-89 0.24800637975242523 1101 90-91 0.5458528118566477 1101 92-93 0.6138219968280332 1101 94-95 0.5392932713565166 1101 96-97 0.48211947373076924 1101 98-99 0.405147260724835 1101 100-101 0.6571472665601767 1102 1 0.08432534574456696 1102 2 0.20538765209177967 1102 3 0.24793721399388602 1102 4 0.19184367700535887 1102 5 0.14814810836157477 1102 6 0.14041565456840743 1102 7 0.19347291350408113 1102 8 0.20544851815439813 1102 9 0.23309645903946574 1102 10-11 0.22813958551121516 1102 12-13 0.24470386130349198 1102 14-15 0.3506846620734123 1102 16-17 0.4301901647498312 1102 18-19 0.47196825927826325 1102 20-21 0.2831157527018391 1102 22-23 0.3904199198664031 1102 24-25 0.36877680909729094 1102 26-27 0.3457353472358946 1102 28-29 0.29346133005618924 1102 30-31 0.3317774832085192 1102 32-33 0.32519785036214444 1102 34-35 0.33867243615760145 1102 36-37 0.2705194588764357 1102 38-39 0.3165436148248446 1102 40-41 0.262544950492277 1102 42-43 0.3080179768949165 1102 44-45 0.32748378159878655 1102 46-47 0.33310867505154107 1102 48-49 0.37161860338640196 1102 50-51 0.26993285688595137 1102 52-53 0.286338112313409 1102 54-55 0.291966561764454 1102 56-57 0.40250333348105016 1102 58-59 0.3943494089445281 1102 60-61 0.4288260188716322 1102 62-63 0.36243130349177477 1102 64-65 0.4548877153090416 1102 66-67 0.37607327979879557 1102 68-69 0.4057097438209212 1102 70-71 0.41233545775126146 1102 72-73 0.29637302583483205 1102 74-75 0.3914521569966567 1102 76-77 0.3099521643660381 1102 78-79 0.19055463577572596 1102 80-81 0.2849498181024046 1102 82-83 0.38088155479545094 1102 84-85 0.37033840274056473 1102 86-87 0.2850754205190107 1102 88-89 0.3600264392740016 1102 90-91 0.5294965609517099 1102 92-93 0.5559894299223522 1102 94-95 0.40877473808764364 1102 96-97 0.25245122115426355 1102 98-99 0.29441199150872066 1102 100-101 0.9822371641941423 1103 1 0.05765932692793285 1103 2 0.10218275502747787 1103 3 0.19134171706307512 1103 4 0.07222362400099058 1103 5 0.0824482726256548 1103 6 0.10946615362604462 1103 7 0.12406976239561374 1103 8 0.21094688957875718 1103 9 0.12677744915890088 1103 10-11 0.16447374314846996 1103 12-13 0.1662450453858213 1103 14-15 0.25484437074548083 1103 16-17 0.30147651361195926 1103 18-19 0.17147060816085258 1103 20-21 0.1693321465469282 1103 22-23 0.1645271441614682 1103 24-25 0.1672506960525766 1103 26-27 0.18153698919474692 1103 28-29 0.20798340000523297 1103 30-31 0.19859673560663538 1103 32-33 0.17349404739721308 1103 34-35 0.3493611092669653 1103 36-37 0.34541791264594934 1103 38-39 0.33843676901059894 1103 40-41 0.39925775942510455 1103 42-43 0.3421488477161745 1103 44-45 0.3692743708951127 1103 46-47 0.4473417697885296 1103 48-49 0.4270848806610985 1103 50-51 0.46252008861423377 1103 52-53 0.38060157075852175 1103 54-55 0.42192033425486386 1103 56-57 0.43219268086591 1103 58-59 0.43431544796386135 1103 60-61 0.38106395714004293 1103 62-63 0.42819626520469 1103 64-65 0.4267480081543056 1103 66-67 0.3752066951363062 1103 68-69 0.32050774673795956 1103 70-71 0.1780367426254088 1103 72-73 0.24816718683067762 1103 74-75 0.3428894037704069 1103 76-77 0.20467158989355028 1103 78-79 0.11053424554837221 1103 80-81 0.1967419673534181 1103 82-83 0.37097490487059304 1103 84-85 0.45388676261443806 1103 86-87 0.1735127465691484 1103 88-89 0.3052918063194028 1103 90-91 0.41368825295698386 1103 92-93 0.60126766872499 1103 94-95 0.5324058352302004 1103 96-97 0.5679843633718349 1103 98-99 0.42586374671347116 1103 100-101 0.6884584851092121 1104 1 0.0433774937477267 1104 2 0.10790675056208698 1104 3 0.07470715772379322 1104 4 0.09625768740555429 1104 5 0.08963389249465337 1104 6 0.012290610518249423 1104 7 -0.040946587643404087 1104 8 0.04037202023087616 1104 9 0.01518225834258402 1104 10-11 0.09094104801001635 1104 12-13 0.15939087192721502 1104 14-15 0.22858576351195126 1104 16-17 0.17734662218411756 1104 18-19 0.22108062762283254 1104 20-21 0.23667874761584784 1104 22-23 0.24604666047692092 1104 24-25 0.29450438345563157 1104 26-27 0.3619957288092692 1104 28-29 0.312724170383504 1104 30-31 0.162955379393118 1104 32-33 0.1672054568594845 1104 34-35 0.2085297270712161 1104 36-37 0.13297632328384168 1104 38-39 0.16801428597808155 1104 40-41 0.3024773834236001 1104 42-43 0.4240803257399506 1104 44-45 0.37181208465582216 1104 46-47 0.39558673387772103 1104 48-49 0.33426457302351054 1104 50-51 0.34432585410231553 1104 52-53 0.3777650118736844 1104 54-55 0.32147404240420485 1104 56-57 0.22451804170287204 1104 58-59 0.3883977711512756 1104 60-61 0.27764847844449037 1104 62-63 0.16092079421490268 1104 64-65 0.2512703499145701 1104 66-67 0.22695283978722358 1104 68-69 0.34281937551797625 1104 70-71 0.30566904951218277 1104 72-73 0.14938553641525942 1104 74-75 0.19429422283327114 1104 76-77 0.19030874745655524 1104 78-79 0.1782389668073776 1104 80-81 0.24043188960428097 1104 82-83 0.2196597393449693 1104 84-85 0.26280617816228613 1104 86-87 0.13748289169426542 1104 88-89 0.2967762021582985 1104 90-91 0.49319587474255044 1104 92-93 0.49885813729438766 1104 94-95 0.4281039473193502 1104 96-97 0.36591368773487787 1104 98-99 0.360666113764438 1104 100-101 0.4880346683877157 1105 1 0.04797356229806837 1105 2 0.10533138755481275 1105 3 0.12345536858187245 1105 4 0.09261963511848847 1105 5 0.03901580537291238 1105 6 0.06629437686930117 1105 7 0.1162142357968392 1105 8 0.07786248387758121 1105 9 0.13673375965717582 1105 10-11 0.14395128124465373 1105 12-13 0.14960480542339383 1105 14-15 0.11177106823402738 1105 16-17 0.11664440862362824 1105 18-19 0.15317514250642006 1105 20-21 0.09575233352877177 1105 22-23 0.05089438236025501 1105 24-25 0.1805153120069889 1105 26-27 0.18570469902079623 1105 28-29 0.015272481126871185 1105 30-31 0.06479861019384003 1105 32-33 0.11899844774151092 1105 34-35 0.13126171783621743 1105 36-37 0.10839310651463308 1105 38-39 0.06201924456887298 1105 40-41 0.16582762090148861 1105 42-43 0.28953419357219445 1105 44-45 0.22368826633437777 1105 46-47 0.22166868361839676 1105 48-49 0.18542940063690594 1105 50-51 0.16915712558061813 1105 52-53 0.2144841026672779 1105 54-55 0.13835830064645904 1105 56-57 0.13203356428877555 1105 58-59 0.17197958164085492 1105 60-61 0.22783906469474857 1105 62-63 0.3561485652022256 1105 64-65 0.2156856705394503 1105 66-67 0.12830235503694354 1105 68-69 0.2091494300355663 1105 70-71 0.12814932435943405 1105 72-73 0.1250013391931475 1105 74-75 0.16299229703752616 1105 76-77 0.23026583308440252 1105 78-79 0.19304644355869982 1105 80-81 0.2609272261006481 1105 82-83 0.2711417308922748 1105 84-85 0.15972297109371425 1105 86-87 0.2689539215202892 1105 88-89 0.376808110999832 1105 90-91 0.27660002273548656 1105 92-93 0.3583325003849005 1105 94-95 0.36818075759926927 1105 96-97 0.3690319735632457 1105 98-99 0.40856160722339396 1105 100-101 0.951100583286209 1106 1 0.02911263723525792 1106 2 -0.032425277997674584 1106 3 -0.04769675609603752 1106 4 -0.03752856013077377 1106 5 -0.011464145837138062 1106 6 0.020891222083903926 1106 7 0.07936367799446487 1106 8 0.049933681425514465 1106 9 0.04847151487208379 1106 10-11 0.1336166602643658 1106 12-13 0.009001631175209468 1106 14-15 0.08092753154274845 1106 16-17 0.06283145450443328 1106 18-19 0.15925696238615927 1106 20-21 0.166333358274926 1106 22-23 0.03198835137331457 1106 24-25 0.07424182594917994 1106 26-27 -0.020904913059190733 1106 28-29 0.017349166940007876 1106 30-31 0.024475953994361532 1106 32-33 0.09283398957774835 1106 34-35 0.19738624705729535 1106 36-37 0.10447478435225577 1106 38-39 0.2637085332038751 1106 40-41 0.05549044618546617 1106 42-43 0.1055900439238826 1106 44-45 0.021401494201953142 1106 46-47 0.1454809245048878 1106 48-49 0.18613364237685204 1106 50-51 0.07394690628574097 1106 52-53 0.07352393429600568 1106 54-55 0.28034049219296264 1106 56-57 0.22433584923248873 1106 58-59 0.1905157742717094 1106 60-61 0.1739690670701748 1106 62-63 0.08000511113714026 1106 64-65 0.13957165243422054 1106 66-67 0.19937478502680506 1106 68-69 0.3306525498242081 1106 70-71 0.2154505938531983 1106 72-73 0.10366774816923652 1106 74-75 0.3211311737201967 1106 76-77 0.19744368007664548 1106 78-79 0.09646929565539608 1106 80-81 0.2030004152602416 1106 82-83 0.24479760457513322 1106 84-85 0.35522753487636294 1106 86-87 0.32787464933195665 1106 88-89 0.32652240992533166 1106 90-91 0.5074854618480806 1106 92-93 0.4971789613302704 1106 94-95 0.4570039931492076 1106 96-97 0.4569679748301425 1106 98-99 0.5245122406405223 1106 100-101 0.5948886739924824 1107 1 0.01798652529193845 1107 2 0.06250995339148346 1107 3 0.06046237145979916 1107 4 0.03050583258995232 1107 5 0.07304131966041894 1107 6 0.08042729882031807 1107 7 0.02345626546310342 1107 8 -0.01727396931695324 1107 9 0.012667019711045668 1107 10-11 -0.0030109194282204044 1107 12-13 0.10182786747171235 1107 14-15 0.10249263250417329 1107 16-17 0.12519839500254903 1107 18-19 0.1340472952774192 1107 20-21 0.08875954940990738 1107 22-23 0.09704248158477924 1107 24-25 0.05334476558222434 1107 26-27 0.08562696874485454 1107 28-29 0.07117358405431418 1107 30-31 -0.039644573186826904 1107 32-33 0.0164388326732805 1107 34-35 0.07144699883752281 1107 36-37 0.030489487288072326 1107 38-39 0.1194183641026143 1107 40-41 0.07349088825945671 1107 42-43 -0.07952804389935864 1107 44-45 -0.07551090517850412 1107 46-47 0.09764851825479326 1107 48-49 -0.15123822772336126 1107 50-51 -0.07449422631419367 1107 52-53 -5.84523311005114E-4 1107 54-55 0.07100008885609554 1107 56-57 0.06941149477184183 1107 58-59 -0.09206492013429823 1107 60-61 0.13627868106642893 1107 62-63 0.09629442471389638 1107 64-65 0.1580363517125889 1107 66-67 -0.06037612694958483 1107 68-69 0.048524106656159915 1107 70-71 0.1878526935456577 1107 72-73 0.036919743067898025 1107 74-75 0.00792007861704036 1107 76-77 -0.1276392485522635 1107 78-79 -0.21523262561726852 1107 80-81 -0.09569157047889831 1107 82-83 0.024553636976925475 1107 84-85 -0.053884198530759875 1107 86-87 0.00459659114992661 1107 88-89 0.18197892288382178 1107 90-91 -0.05420540757471315 1107 92-93 0.13930447854094297 1107 94-95 0.21072894361465444 1107 96-97 -0.05001154664861218 1107 98-99 -0.08395220420677063 1107 100-101 0.38252801476156506 1108 1 -0.09283745088550432 1108 2 -0.04632383288745956 1108 3 0.02788676956551228 1108 4 0.015185418789307903 1108 5 -0.035093152463609556 1108 6 -0.06947499269193713 1108 7 0.10209655122290684 1108 8 0.047362639001704565 1108 9 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#Quality Count 3 12.0 4 23.0 5 37.0 6 83.0 7 201.0 8 431.0 9 815.0 10 1295.0 11 2072.0 12 3019.0 13 4462.0 14 5758.0 15 7428.0 16 9183.0 17 12060.0 18 11435.0 19 8490.0 20 8265.0 21 8749.0 22 9853.0 23 11410.0 24 13730.0 25 16883.0 26 21247.0 27 26714.0 28 33396.0 29 41700.0 30 52093.0 31 65236.0 32 82232.0 33 106453.0 34 146313.0 35 217627.0 36 370573.0 37 665816.0 38 685952.0 39 115369.0 40 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38737623089577 18.211803045079662 13.244911427692314 24.155909296332258 2 15.985344483808467 19.23713656319179 39.68674448764082 25.090774465358916 3 20.295806783234074 22.937297666193864 28.197651301225967 28.5692442493461 4 13.171777154118535 15.20762213768603 35.7271799276189 35.893420780576534 5 14.423409199022002 36.964786869033006 33.84112883286559 14.770675099079403 6 34.82324754886014 34.62725757174853 16.454374314107376 14.095120565283963 7 30.65027320034065 30.857360359852347 19.60044359811861 18.89192284168839 8 27.176132165635025 35.64639099385932 18.644170111492343 18.53330672901331 9 26.53397762056167 15.944156953921029 19.10768532294608 38.41418010257122 10-11 26.15915201437793 25.85773815074995 26.506617477485683 21.476492357386437 12-13 26.291299750060233 22.590116309017297 27.414447274167248 23.704136666755225 14-15 22.993723404716775 24.39771578778428 24.958466897141047 27.6500939103579 16-17 23.380889475842594 27.52050959180712 25.61713127241253 23.481469659937765 18-19 23.29450403908522 26.511439857609055 27.213128798164306 22.980927305141428 20-21 23.997800804347495 25.757175399950693 27.329940075171717 22.91508372053009 22-23 23.487384265702165 25.685676238204262 27.12950385076122 23.697435645332348 24-25 23.415503903990746 25.765453296703296 27.411617987275882 23.407424812030076 26-27 23.284763802354817 26.132116257448274 27.190364269674944 23.39275567052196 28-29 23.14489634691535 26.361983584895327 27.172707787387683 23.320412280801637 30-31 23.425436612341162 26.060446244751606 27.07474566152944 23.43937148137779 32-33 23.270613487640563 25.885229658885155 27.21405465312864 23.630102200345643 34-35 23.48822678771564 26.19496581964797 27.100646194181415 23.216161198454976 36-37 23.66462350716636 25.902893473878414 27.09182867534657 23.34065434360865 38-39 23.41417119727045 26.2578739968917 27.027593170847908 23.300361634989944 40-41 23.603596732353004 26.024040509597928 27.023116764394008 23.349245993655057 42-43 23.496575211861153 25.96670264033209 26.915832648170863 23.620889499635894 44-45 23.427774097667616 26.12862150704578 27.091561241300653 23.352043153985953 46-47 23.62544866975755 26.294302777545298 26.742895711949 23.337352840748153 48-49 23.639105605394175 26.314158825737877 26.654042369123687 23.39269319974426 50-51 23.582590734111342 26.396316086089094 26.68444494571624 23.336648234083327 52-53 23.7801832890411 26.35724824597886 26.442109304296572 23.420459160683464 54-55 23.640378613892597 26.249923412939896 26.613221700877965 23.496476272289545 56-57 23.438163093417774 26.596846431631114 26.504795057819035 23.460195417132073 58-59 23.571807605025096 26.334841170980404 26.634613368690523 23.458737855303976 60-61 23.548343544732397 26.32907086721193 26.72859234512872 23.393993242926953 62-63 23.45322627986299 26.3551214038984 26.804972983602195 23.386679332636415 64-65 23.43764063647119 26.363439523922615 26.6636808809586 23.535238958647593 66-67 23.46528136237253 26.578275982227158 26.436615207549842 23.51982744785047 68-69 23.522484265135184 26.249860833380325 26.75984323693605 23.467811664548442 70-71 23.469887914680978 26.13449876810253 26.631902674316382 23.763710642900108 72-73 23.484369665397463 26.401846566596713 26.467942546200764 23.645841221805057 74-75 23.402186470257384 26.48234138258603 26.473286515513646 23.64218563164295 76-77 23.53376510943219 26.435714077473794 26.4398788873294 23.59064192576462 78-79 23.509376518593648 26.443873864322626 26.438554828942358 23.608194788141372 80-81 23.468038491388022 26.368249123330838 26.472372224634068 23.69134016064707 82-83 23.607592786180792 26.499095597707978 26.443808495164234 23.449503120947 84-85 23.587012609108594 26.538355529328626 26.22110807975196 23.65352378181082 86-87 23.469753906625336 26.374509460821738 26.49466181545503 23.661074817097898 88-89 23.501358323949127 26.416251534874252 26.349594138802622 23.732796002374 90-91 23.34446450714643 26.623519771430225 26.393952908245772 23.63806281317757 92-93 23.464936090321643 26.464619074971317 26.447182324427597 23.623262510279435 94-95 23.38242842683632 26.47232865818392 26.49819259687681 23.64705031810295 96-97 23.520653297057528 26.222505542856535 26.484994840764987 23.771846319320954 98-99 23.1887761833688 26.375314804097595 26.388834164798112 24.047074847735487 100-101 23.237622119635766 26.425935504999735 26.38187176548114 23.954570609883362 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 71.0 1 54.0 2 90.5 3 149.0 4 110.0 5 97.5 6 237.5 7 514.0 8 796.0 9 914.0 10 793.0 11 715.5 12 799.5 13 862.0 14 955.0 15 1060.5 16 1182.0 17 1337.0 18 1505.0 19 1695.0 20 1928.5 21 2353.0 22 2879.0 23 3573.5 24 4595.5 25 6001.5 26 8157.0 27 10985.0 28 13865.5 29 17080.5 30 20827.5 31 26087.5 32 32496.0 33 37351.0 34 42280.5 35 48031.5 36 54550.5 37 60950.5 38 66044.5 39 70534.0 40 75523.5 41 81045.5 42 84647.0 43 88660.5 44 94145.5 45 99449.5 46 104041.5 47 107400.5 48 109847.5 49 110517.5 50 109063.5 51 108867.5 52 110192.0 53 109326.0 54 108367.5 55 102753.5 56 93420.5 57 85062.5 58 79016.0 59 72838.5 60 63508.0 61 53762.0 62 43592.0 63 33736.5 64 24578.0 65 17637.5 66 12671.5 67 9883.0 68 7897.5 69 6215.0 70 4726.5 71 2971.0 72 2016.0 73 1665.0 74 1173.0 75 747.0 76 494.5 77 254.0 78 138.5 79 69.0 80 28.5 81 14.0 82 8.0 83 5.0 84 5.0 85 5.5 86 3.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26015121085803267 2 0.019772504146080852 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.1688304364988136E-4 12-13 5.422076091247034E-5 14-15 0.0 16-17 6.687227179204675E-4 18-19 0.0 20-21 7.229434788329378E-5 22-23 7.229434788329378E-5 24-25 0.002458007828031989 26-27 0.002024241740732226 28-29 0.0018977266319364621 30-31 9.579001094536428E-4 32-33 0.0014097397837242289 34-35 0.0014278133706950523 36-37 3.614717394164689E-5 38-39 1.6266228273741103E-4 40-41 4.5183967427058614E-4 42-43 0.003343613589602338 44-45 0.0034520551114272783 46-47 0.013537116641146762 48-49 0.013500969467205115 50-51 0.00618116674402162 52-53 0.006560712070408911 54-55 3.072509785039986E-4 56-57 0.0028194795674484577 58-59 0.0012290039140159944 60-61 0.001210930327045171 62-63 0.0 64-65 9.75973696424466E-4 66-67 0.0 68-69 0.0 70-71 1.4458869576658757E-4 72-73 0.0013193718488701117 74-75 0.0 76-77 0.18932082351937563 78-79 0.10153741160208613 80-81 0.0011205623921910538 82-83 0.0 84-85 5.602811960955269E-4 86-87 0.11962907215988039 88-89 0.5724085729529493 90-91 0.30537132545903295 92-93 0.2865205742484641 94-95 0.002458007828031989 96-97 0.002512228588944459 98-99 0.002349566306207048 100-101 7.590906527745848E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2766468.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.517479000849896 #Duplication Level Percentage of deduplicated Percentage of total 1 67.45267311786128 30.702756321934434 2 15.58664587603028 14.18929652711784 3 6.5020525334170225 8.878711189566966 4 3.235071064909287 5.8900911705306225 5 1.8662222191824593 4.247286533627855 6 1.2293041999812446 3.357289686498173 7 0.8321850269359308 2.651527514186456 8 0.5737957547753492 2.089418897501102 9 0.4338081161450442 1.7771266635327274 >10 2.100172710809795 16.73854926519936 >50 0.12943797115164027 4.0356649240548 >100 0.056170504626803446 4.372184799039226 >500 0.002063984145833041 0.6632321928090087 >1k 3.969200280307902E-4 0.40686431440125376 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3859 0.13949194424081537 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2822 0.10200732486332754 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.8073586970823446E-5 0.0 16-17 1.8073586970823446E-5 0.0 0.0 5.422076091247034E-5 0.0 18-19 5.422076091247034E-5 0.0 0.0 7.229434788329378E-5 0.0 20-21 7.229434788329378E-5 0.0 0.0 1.2651510879576412E-4 0.0 22-23 7.229434788329378E-5 0.0 0.0 3.6147173941646895E-4 0.0 24-25 1.4458869576658757E-4 0.0 0.0 5.964283700371738E-4 0.0 26-27 1.4458869576658757E-4 0.0 0.0 7.410170658037614E-4 0.0 28-29 1.62662282737411E-4 0.0 0.0 0.0011024888052202303 0.0 30-31 1.8073586970823448E-4 0.0 0.0 0.0026206701107693996 0.0 32-33 1.8073586970823448E-4 0.0 0.0 0.005060604351830565 0.0 34-35 1.8073586970823448E-4 0.0 0.0 0.008042746202016435 0.0 36-37 1.8073586970823448E-4 0.0 0.0 0.01431428088089217 0.0 38-39 1.8073586970823448E-4 0.0 0.0 0.024706593389115655 0.0 40-41 1.8073586970823448E-4 0.0 0.0 0.037213515572925474 0.0 42-43 1.8073586970823448E-4 0.0 0.0 0.04968429058279365 0.0 44-45 1.8073586970823448E-4 0.0 0.0 0.06501069233405193 0.0 46-47 1.8073586970823448E-4 0.0 0.0 0.08406025300129985 0.0 48-49 1.8073586970823448E-4 0.0 0.0 0.10480873084380517 0.0 50-51 1.8073586970823448E-4 0.0 0.0 0.12870201281923377 0.0 52-53 1.8073586970823448E-4 0.0 0.0 0.15198079283765437 0.0 54-55 1.8073586970823448E-4 0.0 0.0 0.17760913916228202 0.0 56-57 1.8073586970823448E-4 0.0 0.0 0.2092198427742522 1.8073586970823446E-5 58-59 1.8073586970823448E-4 0.0 0.0 0.24648757910809016 3.614717394164689E-5 60-61 1.8073586970823448E-4 0.0 0.0 0.287351959249122 3.614717394164689E-5 62-63 1.8073586970823448E-4 0.0 0.0 0.3300598452611778 7.229434788329378E-5 64-65 1.8073586970823448E-4 0.0 0.0 0.37251470105564205 7.229434788329378E-5 66-67 1.8073586970823448E-4 0.0 0.0 0.42368102577004324 7.229434788329378E-5 68-69 1.8073586970823448E-4 0.0 0.0 0.4850047063620472 7.229434788329378E-5 70-71 1.8073586970823448E-4 0.0 0.0 0.5560519767443541 7.229434788329378E-5 72-73 1.8073586970823448E-4 0.0 0.0 0.6294126662589266 7.229434788329378E-5 74-75 1.8073586970823448E-4 0.0 0.0 0.7036589615350692 7.229434788329378E-5 76-77 1.8073586970823448E-4 0.0 0.0 0.787321595623011 7.229434788329378E-5 78-79 1.8073586970823448E-4 0.0 0.0 0.8809608497188472 7.229434788329378E-5 80-81 1.8073586970823448E-4 0.0 0.0 0.9922218511112364 7.229434788329378E-5 82-83 1.8073586970823448E-4 0.0 0.0 1.1069529812020236 7.229434788329378E-5 84-85 1.8073586970823448E-4 0.0 0.0 1.2246120323820842 7.229434788329378E-5 86-87 1.8073586970823448E-4 0.0 0.0 1.3532778980273763 7.229434788329378E-5 88-89 1.8073586970823448E-4 0.0 0.0 1.5029271981457946 7.229434788329378E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3405 0.0 44.566597 1 GTATCAA 5910 0.0 39.762653 1 ATCAACG 7230 0.0 32.41379 3 TCAACGC 7265 0.0 32.32293 4 CAACGCA 7515 0.0 31.247652 5 TATCAAC 7585 0.0 31.029108 2 AACGCAG 7855 0.0 30.076292 6 TACATGG 9940 0.0 26.255453 2 GTACATG 10510 0.0 25.572998 1 ACATGGG 9930 0.0 25.368013 3 ACGCAGA 9270 0.0 25.280659 7 CGCAGAG 9370 0.0 25.061483 8 GTACCGT 250 3.8926373E-10 22.771017 6 GTATAGG 765 0.0 21.76419 1 TAGATCG 110 0.0028997017 21.563463 5 CATGGGG 6105 0.0 21.446903 4 TAGCGCG 155 3.947509E-5 21.424343 4 GTGTACG 300 1.6370905E-10 20.613798 1 GTACCTG 2570 0.0 20.545927 1 GCAGAGT 11470 0.0 20.14219 9 >>END_MODULE