##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936330_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 961482 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82892971475285 33.0 31.0 34.0 30.0 34.0 2 31.85884395131682 33.0 31.0 34.0 30.0 34.0 3 31.98891086884622 34.0 31.0 34.0 30.0 34.0 4 35.41403999242836 37.0 35.0 37.0 33.0 37.0 5 35.479782252813884 37.0 35.0 37.0 33.0 37.0 6 35.660397178522324 37.0 35.0 37.0 35.0 37.0 7 35.63206591491053 37.0 35.0 37.0 33.0 37.0 8 35.65683704947155 37.0 35.0 37.0 35.0 37.0 9 37.26611522628609 39.0 37.0 39.0 34.0 39.0 10-11 37.21853139216335 39.0 37.0 39.0 34.0 39.0 12-13 37.21992819418357 39.0 37.0 39.0 34.0 39.0 14-15 38.25908129325354 40.0 38.0 41.0 34.0 41.0 16-17 38.35067115141001 40.0 38.0 41.0 34.0 41.0 18-19 38.41870154615479 40.0 38.0 41.0 34.0 41.0 20-21 38.31703557632905 40.0 38.0 41.0 34.0 41.0 22-23 38.17571363790482 40.0 38.0 41.0 33.5 41.0 24-25 38.28975945467518 40.0 38.0 41.0 34.0 41.0 26-27 38.163694172121794 40.0 38.0 41.0 33.5 41.0 28-29 38.16477219542331 40.0 38.0 41.0 34.0 41.0 30-31 38.1668538776597 40.0 38.0 41.0 34.0 41.0 32-33 37.933013826571894 40.0 38.0 41.0 33.0 41.0 34-35 37.59191955751642 40.0 37.0 41.0 33.0 41.0 36-37 37.33343109907414 40.0 37.0 41.0 32.0 41.0 38-39 37.17806157577573 40.0 37.0 41.0 32.0 41.0 40-41 37.253369277843994 40.0 37.0 41.0 32.0 41.0 42-43 37.06115871123953 40.0 37.0 41.0 31.0 41.0 44-45 36.85890167470634 40.0 37.0 41.0 31.0 41.0 46-47 36.508498859053006 40.0 36.5 41.0 30.0 41.0 48-49 36.17558935060667 39.5 36.5 41.0 29.0 41.0 50-51 35.77370507196183 39.5 35.5 40.5 28.0 41.0 52-53 36.04800661894866 39.0 36.5 40.5 29.0 41.0 54-55 36.27214446032271 40.0 36.5 41.0 29.5 41.0 56-57 36.04634772153821 40.0 36.0 41.0 28.5 41.0 58-59 35.87187695661489 39.0 36.0 41.0 28.0 41.0 60-61 35.35774356670224 39.0 35.0 41.0 27.0 41.0 62-63 35.27303423257014 39.0 35.0 40.5 27.0 41.0 64-65 34.99090674604413 38.5 35.0 40.0 26.5 41.0 66-67 34.44251322437654 37.5 34.0 40.0 26.0 41.0 68-69 33.930402753249666 37.0 34.0 39.0 25.5 41.0 70-71 33.219389442548064 36.0 33.5 39.0 23.5 40.5 72-73 32.69265415265184 36.0 32.5 37.5 22.5 40.0 74-75 32.363432700768186 35.0 32.5 37.0 22.0 39.5 76-77 32.02600620708448 35.0 32.0 36.5 21.5 39.0 78-79 31.537154621719388 35.0 32.0 36.0 20.0 39.0 80-81 30.970064442184047 35.0 31.0 36.0 17.5 37.5 82-83 30.480061509211822 34.5 30.5 35.5 10.5 37.0 84-85 30.116477999588135 34.0 31.0 35.0 7.0 36.5 86-87 30.084307350527624 34.0 31.0 35.0 4.0 36.0 88-89 29.63329734722023 34.0 30.5 35.0 2.0 36.0 90-91 29.11820086075454 34.0 29.5 35.0 2.0 35.5 92-93 28.724994331667155 34.0 29.0 35.0 2.0 35.0 94-95 28.22136971882989 34.0 29.0 35.0 2.0 35.0 96-97 27.754981372506194 34.0 28.5 35.0 2.0 35.0 98-99 27.433417890298518 34.0 29.0 35.0 2.0 35.0 100-101 23.90174543049168 29.5 14.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3423317558273844 1101 2 0.2627879421339543 1101 3 0.3280999220448422 1101 4 0.21359824098394142 1101 5 0.22510427905965713 1101 6 0.13556502376589208 1101 7 0.3326526376959791 1101 8 0.21799992523680345 1101 9 0.2542721865979871 1101 10-11 0.499570719967771 1101 12-13 0.3632511176371622 1101 14-15 0.45750865080982805 1101 16-17 0.48764868950425466 1101 18-19 0.4787339311746308 1101 20-21 0.5605948605330013 1101 22-23 0.5644603282767164 1101 24-25 0.5861319795084512 1101 26-27 0.5722661160325373 1101 28-29 0.5624321369036522 1101 30-31 0.637137173364188 1101 32-33 0.6956262313403769 1101 34-35 0.7010919077508504 1101 36-37 0.751338634957591 1101 38-39 0.7133906745951464 1101 40-41 0.620234115904502 1101 42-43 0.705220023689705 1101 44-45 0.7062904080339223 1101 46-47 0.8029428036234734 1101 48-49 1.0035134023612926 1101 50-51 0.8976134170904544 1101 52-53 0.9710070822965733 1101 54-55 1.0550970974846976 1101 56-57 1.1393676831577082 1101 58-59 1.056787308824532 1101 60-61 1.0416921411309303 1101 62-63 1.038449002670255 1101 64-65 1.1161461127556294 1101 66-67 1.0772870740049711 1101 68-69 1.1361686993588975 1101 70-71 1.2959576382976863 1101 72-73 1.2024086402775183 1101 74-75 1.2121593360628395 1101 76-77 1.197229431462496 1101 78-79 1.2987148791327705 1101 80-81 1.1910349173730488 1101 82-83 1.328569987776124 1101 84-85 1.4448481060015155 1101 86-87 1.1737087742965073 1101 88-89 1.2942549101702987 1101 90-91 1.5639523113830336 1101 92-93 1.4748513386021642 1101 94-95 1.7236689457001866 1101 96-97 2.047505055933943 1101 98-99 2.2272476324090036 1101 100-101 2.392535533537135 1102 1 0.14003257456607443 1102 2 0.2817459982589128 1102 3 0.18353800118558183 1102 4 0.24662050156581472 1102 5 0.14829807918192728 1102 6 0.1786626850262465 1102 7 0.003952430314143385 1102 8 0.03539302769301855 1102 9 0.033794129514035376 1102 10-11 0.20024560998347596 1102 12-13 0.2504078287770355 1102 14-15 0.3568160793949531 1102 16-17 0.28614666962916147 1102 18-19 0.25257085473105434 1102 20-21 0.36451563587733915 1102 22-23 0.40894193362788656 1102 24-25 0.3450925529851503 1102 26-27 0.24747379755157795 1102 28-29 0.36678445826158423 1102 30-31 0.3887704551517501 1102 32-33 0.122784860462545 1102 34-35 0.06624127062110574 1102 36-37 0.09488522394597965 1102 38-39 -0.018673194214862576 1102 40-41 0.015284522876370943 1102 42-43 0.1765132232437736 1102 44-45 0.08591004817250081 1102 46-47 0.20234762974639153 1102 48-49 0.3951968513729298 1102 50-51 0.1201817751777412 1102 52-53 0.2784326705777005 1102 54-55 0.24043112608661232 1102 56-57 0.12680190235911226 1102 58-59 0.2520334099261774 1102 60-61 0.3898538749869189 1102 62-63 0.4164488468341929 1102 64-65 0.26582964567899126 1102 66-67 0.37397495835064376 1102 68-69 0.545948610891962 1102 70-71 0.6075773143984335 1102 72-73 0.6217604821398837 1102 74-75 0.5226851628930191 1102 76-77 0.8020097026172337 1102 78-79 0.5752040125644022 1102 80-81 0.6949652826288037 1102 82-83 0.8286868648214671 1102 84-85 0.9020246562706333 1102 86-87 0.7402957248245521 1102 88-89 0.6544699639337388 1102 90-91 0.9422937961410689 1102 92-93 1.0591706347026673 1102 94-95 1.1671618911216015 1102 96-97 1.219750353880503 1102 98-99 1.2576311689285866 1102 100-101 0.957089123160852 1103 1 0.37870975220844727 1103 2 0.33475413393770737 1103 3 0.25019548796080215 1103 4 0.21005297772678233 1103 5 0.2439209921909864 1103 6 0.2591158274178369 1103 7 0.32639611741754493 1103 8 0.1383655912924553 1103 9 0.2916285931215086 1103 10-11 0.37776749828714884 1103 12-13 0.41455576272772277 1103 14-15 0.4866900280105142 1103 16-17 0.5025281859177326 1103 18-19 0.3899048995059715 1103 20-21 0.45652826970294313 1103 22-23 0.537053056213864 1103 24-25 0.5942348068779992 1103 26-27 0.5579922319210908 1103 28-29 0.6062204113064382 1103 30-31 0.5132268334550716 1103 32-33 0.61657982758706 1103 34-35 0.5395378270143283 1103 36-37 0.7032624486353001 1103 38-39 0.6999832139231117 1103 40-41 0.7353638141887515 1103 42-43 0.6991904238358728 1103 44-45 0.717125121554254 1103 46-47 0.9237838237633937 1103 48-49 0.790346713100817 1103 50-51 0.7609970069126248 1103 52-53 0.9244589250672419 1103 54-55 0.879942526364303 1103 56-57 0.8497671220453853 1103 58-59 0.9365592685328963 1103 60-61 0.7575745079404328 1103 62-63 0.8900695681441633 1103 64-65 0.687014540167624 1103 66-67 0.8735876816801849 1103 68-69 0.8381617679713784 1103 70-71 0.913251921671197 1103 72-73 0.7988262445872394 1103 74-75 0.7964366976561124 1103 76-77 0.8397089349488098 1103 78-79 0.7813097878636093 1103 80-81 0.7998211583330708 1103 82-83 0.8661150034837313 1103 84-85 0.9686290717984214 1103 86-87 1.0101816646733148 1103 88-89 0.9872094730390479 1103 90-91 1.1502513276682578 1103 92-93 1.2271396984684877 1103 94-95 1.1994105629060563 1103 96-97 1.4030053712649213 1103 98-99 1.6013392139559528 1103 100-101 1.5282088977945172 1104 1 0.2074911133379267 1104 2 0.07486318536982495 1104 3 0.2027371644905287 1104 4 0.1739201678838569 1104 5 0.2691581817766817 1104 6 0.21027531513730224 1104 7 0.23929781604051925 1104 8 0.2334482097239956 1104 9 0.16350676460542957 1104 10-11 0.17678826649027712 1104 12-13 0.11131972677993218 1104 14-15 0.4180927864022692 1104 16-17 0.37890527314527134 1104 18-19 0.3117745003309338 1104 20-21 0.3778815903571413 1104 22-23 0.42687111336791617 1104 24-25 0.45992645905617024 1104 26-27 0.37458421141680986 1104 28-29 0.3387389878696325 1104 30-31 0.1092869151348097 1104 32-33 0.28711077310759947 1104 34-35 0.1526188292644619 1104 36-37 0.2682130789188335 1104 38-39 0.19576726596741167 1104 40-41 0.3961799857978363 1104 42-43 0.3362447835804403 1104 44-45 0.18435867609221646 1104 46-47 0.22707495680450762 1104 48-49 0.16125218281374032 1104 50-51 0.09706682921429888 1104 52-53 0.008872433514106604 1104 54-55 0.11635871212177307 1104 56-57 0.09245009200999021 1104 58-59 0.014976036551011873 1104 60-61 0.08449753253958647 1104 62-63 0.11412427887761822 1104 64-65 0.15698458367391765 1104 66-67 0.13672459727688135 1104 68-69 -0.0508067056078616 1104 70-71 0.12296030159898663 1104 72-73 0.17228590671282262 1104 74-75 -0.02645199533199616 1104 76-77 0.07634482886854244 1104 78-79 -0.08918415417696934 1104 80-81 -0.09361358086041704 1104 82-83 0.19324685286868615 1104 84-85 0.14123602137845737 1104 86-87 0.24131669200419026 1104 88-89 0.28075862372755367 1104 90-91 0.5278371498937453 1104 92-93 0.5190665458536223 1104 94-95 0.2775695309443016 1104 96-97 0.39046976221757035 1104 98-99 0.721106690617912 1104 100-101 0.8958248669727951 1105 1 0.07269684228035445 1105 2 0.05571626445459188 1105 3 0.12287091635290892 1105 4 0.04165037842692243 1105 5 0.009486220874180162 1105 6 0.1333549478545848 1105 7 0.17486678331228234 1105 8 0.18712951707930614 1105 9 -0.0032355712771732215 1105 10-11 0.044426499796735186 1105 12-13 0.13762130252612081 1105 14-15 0.2025444585614835 1105 16-17 0.23828316781894188 1105 18-19 0.1454900936689043 1105 20-21 0.18844436058105174 1105 22-23 0.30802086796482087 1105 24-25 0.13356382608056805 1105 26-27 0.19466108090306022 1105 28-29 0.25404882721606015 1105 30-31 0.3425300977298562 1105 32-33 0.2894597804057639 1105 34-35 0.03758726998285056 1105 36-37 0.19329729353761138 1105 38-39 0.3716160005954734 1105 40-41 0.10984506341096534 1105 42-43 0.10310858160104885 1105 44-45 0.4485975351048097 1105 46-47 0.4407517761925348 1105 48-49 0.4471663582671681 1105 50-51 0.4300028991639664 1105 52-53 0.35610150510501626 1105 54-55 0.49420837565468645 1105 56-57 0.7530495226643623 1105 58-59 0.7971471950988942 1105 60-61 0.7604232410252365 1105 62-63 0.525498014353694 1105 64-65 0.6057160805936519 1105 66-67 0.4229251415733728 1105 68-69 0.46493444826940333 1105 70-71 0.6114742364764965 1105 72-73 0.647018259477484 1105 74-75 0.5275614737884453 1105 76-77 0.5775659734567569 1105 78-79 0.7715962302748345 1105 80-81 0.7863606916061663 1105 82-83 0.7969365099305783 1105 84-85 0.7421683183044614 1105 86-87 1.0064198761126129 1105 88-89 0.9581905410028391 1105 90-91 0.9589722925041215 1105 92-93 0.7816322019448627 1105 94-95 0.8496927308649767 1105 96-97 0.9622687586872125 1105 98-99 0.8223162238954096 1105 100-101 0.694897658149241 1106 1 0.04137817757577622 1106 2 0.16907980185858662 1106 3 0.052339035771538533 1106 4 -0.03008848907161621 1106 5 0.08469990039521491 1106 6 0.017660590203448123 1106 7 -0.007756132808424354 1106 8 -0.0585212983298149 1106 9 -0.04653591195490492 1106 10-11 -0.015384867550615411 1106 12-13 -0.07764569608294636 1106 14-15 0.04833739101053425 1106 16-17 0.0010396840364066406 1106 18-19 -0.1017053626175013 1106 20-21 -0.2069862238122866 1106 22-23 -0.20174967853063208 1106 24-25 -0.20001560827345344 1106 26-27 -0.05398830325643189 1106 28-29 -0.07687977157040393 1106 30-31 -0.06285623887587377 1106 32-33 0.03222919802827562 1106 34-35 0.0013071079089570503 1106 36-37 -0.1403174180958331 1106 38-39 -0.0631374112214047 1106 40-41 -0.07903327029193719 1106 42-43 0.08392152130713271 1106 44-45 -0.01706759280052239 1106 46-47 0.0421978926680282 1106 48-49 0.21579024199326113 1106 50-51 0.13243274777403968 1106 52-53 -0.06792383406083502 1106 54-55 -0.07273152194040478 1106 56-57 0.1514534777513532 1106 58-59 0.270536787293544 1106 60-61 0.5012776063365791 1106 62-63 0.3532327594501581 1106 64-65 0.12016464632026214 1106 66-67 0.37130566246760566 1106 68-69 0.5846894727619159 1106 70-71 0.7961621333458879 1106 72-73 0.7397118309492541 1106 74-75 0.5295521202697842 1106 76-77 0.6092512698866983 1106 78-79 0.5330991431701868 1106 80-81 0.8386076023244122 1106 82-83 0.8624338187781184 1106 84-85 0.8141730253014572 1106 86-87 0.8293871488765383 1106 88-89 0.7549493149661899 1106 90-91 0.6101681922763333 1106 92-93 0.684224074059685 1106 94-95 0.7043503396701887 1106 96-97 0.639855843171258 1106 98-99 0.4100936544999598 1106 100-101 0.7393344311853092 1107 1 -0.13331311692926562 1107 2 -0.11873147618216251 1107 3 0.11775887524467521 1107 4 0.006647464828368754 1107 5 -0.18040094339980328 1107 6 0.033291378431478336 1107 7 0.06706893407776704 1107 8 0.11500206038597582 1107 9 0.12890142082962086 1107 10-11 0.05906203510425456 1107 12-13 0.07464386967132697 1107 14-15 0.2660622315126915 1107 16-17 -0.07654572448385721 1107 18-19 0.10816292303798036 1107 20-21 0.174913344000025 1107 22-23 0.25386104384022445 1107 24-25 0.2785550852848928 1107 26-27 0.17368714436653931 1107 28-29 0.0816816608230937 1107 30-31 -0.06983812815349921 1107 32-33 0.010642966293353595 1107 34-35 -0.19169042244708834 1107 36-37 -0.11606860146689257 1107 38-39 -0.08856702636855829 1107 40-41 0.2541397076870666 1107 42-43 -0.1338676328398094 1107 44-45 0.01667906288994203 1107 46-47 0.0807011855266424 1107 48-49 0.014874415691551235 1107 50-51 0.08971047981766844 1107 52-53 0.04625089324930798 1107 54-55 0.07082939594393167 1107 56-57 0.10475662654740603 1107 58-59 0.12469659383809528 1107 60-61 0.16484264029418938 1107 62-63 0.20251501917437764 1107 64-65 -0.25681106884746185 1107 66-67 -0.09250633588596457 1107 68-69 -0.012241480109047131 1107 70-71 -0.07945785018981866 1107 72-73 -0.13913155246297038 1107 74-75 -0.12889612010889806 1107 76-77 -0.09643148376631672 1107 78-79 -0.16463701653345097 1107 80-81 -0.08931738224031349 1107 82-83 0.10812737712345921 1107 84-85 0.06087759414278082 1107 86-87 -0.2705176669291838 1107 88-89 -0.08894088877943673 1107 90-91 0.25028447134609877 1107 92-93 0.050595755475612236 1107 94-95 -0.07561981879863211 1107 96-97 0.2646526120537409 1107 98-99 0.09989608298318586 1107 100-101 0.008355834766287984 1108 1 -0.20666254538807394 1108 2 -0.12992087310766465 1108 3 -0.12135227966269113 1108 4 -0.04481587770218454 1108 5 -0.15211950863432833 1108 6 -0.12278863733517653 1108 7 0.04342880794453663 1108 8 -0.12384303224311566 1108 9 0.11681529548472724 1108 10-11 -0.0885015242177829 1108 12-13 -0.12181848744337742 1108 14-15 -0.03505375193232396 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15 4542.0 16 5930.0 17 7960.0 18 7121.0 19 4473.0 20 4335.0 21 4445.0 22 4916.0 23 5234.0 24 6022.0 25 6954.0 26 7967.0 27 9687.0 28 11816.0 29 14443.0 30 17805.0 31 22431.0 32 28837.0 33 38030.0 34 52628.0 35 78680.0 36 122876.0 37 195600.0 38 229790.0 39 41373.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.930438392854356 20.59096826019502 14.076103526101601 18.402489820849024 2 13.432775272659187 26.782023477035256 34.44154925548569 25.343651994819865 3 26.979525521327407 25.691289297185747 30.442004926190357 16.887180255296485 4 10.59310795307392 16.03075428503672 46.087430454938335 27.28870730695102 5 8.857028144197145 43.515538700611394 36.617932486379324 11.009500668812136 6 25.3621447330785 41.99661960111293 15.98502223262345 16.656213433185115 7 23.882753326829107 35.352590839024536 23.905531991021668 16.859123843124692 8 22.69987403694431 27.40960252466993 21.885326812240155 28.0051966261456 9 20.56807968843619 23.24568520737634 27.19286748552027 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24.09470323639259 26.89202344013552 29.3141429693932 19.699130354078694 36-37 21.47898141085255 25.22685238398763 33.73094325033176 19.563222954828056 38-39 19.297107241561264 29.96635476910699 32.11217863318861 18.624359356143128 40-41 18.96103136021053 31.260767939249618 30.27533003390872 19.502870666631132 42-43 17.67465944354138 31.39013288899409 26.087740280993366 24.847467386471166 44-45 20.755928768552142 25.80506931202194 28.19596434560536 25.243037573820555 46-47 24.513434033931187 29.855063677806864 28.810150820946966 16.821351467314987 48-49 22.226176980484574 28.639104963291306 29.23921775230007 19.895500303924045 50-51 15.286371175179509 29.625027899320955 35.377070756859574 19.71153016863996 52-53 20.26161759072025 33.03240899229458 26.36010467630468 20.3458687406805 54-55 17.755485028431792 27.6693247599851 29.55584112479207 25.019349086791042 56-57 22.431611450512918 26.138955645749167 28.315150907083336 23.11428199665458 58-59 26.82525358038097 25.88926227979096 27.11281276568394 20.17267137414413 60-61 21.086387188335397 29.012632749420515 32.97593841639164 16.92504164585245 62-63 18.36438137995034 29.272946920485232 31.804078570055093 20.558593129509333 64-65 17.587813642549403 34.494670454521696 28.539250916129948 19.37826498679895 66-67 20.907384390489074 28.05703714268847 25.53526525677738 25.500313210045082 68-69 18.213102777188404 28.712037200590746 28.741982045635318 24.332877976585536 70-71 24.235446442846975 28.192837310109088 26.936372632927558 20.635343614116376 72-73 22.045454664216248 24.195597294646408 33.66705213548506 20.091895905652283 74-75 17.847750648047494 34.45125010452379 27.964033363993646 19.73696588343507 76-77 19.41372704555929 27.55483930977038 28.17385542634565 24.857578218324676 78-79 19.977791422263447 28.52537656603133 29.741622793839078 21.75520921786615 80-81 23.679866305256077 27.1055328323961 27.99494986708389 21.219650995263933 82-83 21.248977736204992 25.39932955171936 32.59225195895791 20.75944075311774 84-85 19.249712454921657 28.980154294520368 30.786632406865966 20.98350084369201 86-87 19.377053412368316 30.42031623399664 28.87739909896775 21.325231254667294 88-89 18.23700502003047 30.33143699352178 26.10279956202129 25.32875842442646 90-91 20.70799189701366 25.169705286545124 28.072508658432987 26.049794158008233 92-93 23.94904218885251 29.014401665976692 28.4201865860441 18.6163695591267 94-95 21.806616068610463 26.825835944407633 29.445340930706028 21.92220705627588 96-97 16.290415856708286 27.910351777485342 34.2210007036908 21.578231662115577 98-99 20.376647480127854 30.87418634814818 26.541726934755843 22.207439236968117 100-101 17.840638024656272 26.728357847157863 29.083022404629084 26.347981723556778 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 980.0 1 576.5 2 307.0 3 424.5 4 296.0 5 298.0 6 609.0 7 999.5 8 1271.0 9 1264.0 10 1007.5 11 823.5 12 863.0 13 934.5 14 954.0 15 965.0 16 1304.5 17 1507.5 18 1142.5 19 934.0 20 1078.5 21 1318.0 22 1554.0 23 1922.0 24 2207.5 25 2680.0 26 4195.5 27 6164.0 28 6969.5 29 8004.0 30 11153.5 31 13537.0 32 17148.5 33 22674.5 34 27408.5 35 31776.0 36 38432.0 37 44140.5 38 42656.5 39 39158.5 40 34841.5 41 28903.0 42 23286.5 43 22392.0 44 23464.5 45 22606.0 46 25498.0 47 61862.0 48 136335.5 49 129085.0 50 54840.5 51 19528.5 52 8378.5 53 5564.5 54 4128.5 55 3260.0 56 2912.5 57 2601.5 58 2154.5 59 1820.0 60 1586.5 61 1345.5 62 1081.0 63 750.5 64 499.5 65 387.5 66 266.0 67 223.0 68 170.0 69 93.5 70 68.0 71 56.0 72 41.0 73 49.5 74 43.0 75 33.0 76 28.5 77 14.5 78 7.5 79 9.0 80 10.5 81 6.0 82 6.5 83 9.5 84 8.5 85 6.0 86 5.0 87 7.5 88 6.5 89 2.0 90 3.0 91 5.0 92 2.5 93 2.5 94 4.0 95 6.5 96 9.5 97 6.5 98 2.0 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046698742150139055 2 0.012168714546918195 3 0.009360549651475534 4 0.005720335898123937 5 0.007904464150134896 6 0.005928348112601172 7 0.005824342005362555 8 0.009256543544236918 9 0.014352842798929151 10-11 0.0023921404664881923 12-13 0.008996528276140375 14-15 0.009620564919572078 16-17 0.01903311762466692 18-19 0.01196070233244096 20-21 0.0034842045924936716 22-23 0.009828577134049311 24-25 0.021945288627348198 26-27 0.007124418345845268 28-29 0.01872109930295107 30-31 0.014872873335122237 32-33 0.013312781726542985 34-35 0.26297944215284325 36-37 0.4651153115710955 38-39 0.4614750978177438 40-41 0.4834723894987114 42-43 0.47379982152552 44-45 0.48732061546654015 46-47 0.494185018544289 48-49 0.5021934888016624 50-51 0.5114500323458994 52-53 0.5011534277292762 54-55 0.49075281700541457 56-57 0.4837844078204272 58-59 0.4821203101046094 60-61 0.47884411772659297 62-63 0.47613995893838884 64-65 0.49298894831104484 66-67 0.46350321690889695 68-69 0.4912208444879883 70-71 0.4733317940429462 72-73 0.4824323284263252 74-75 0.49527708267029447 76-77 0.4826923436944217 78-79 0.4830563650697569 80-81 0.4862805543941541 82-83 0.48461645667833614 84-85 0.4862805543941541 86-87 0.4878406460027333 88-89 0.5007374033003218 90-91 0.5246588079652037 92-93 0.5123340842574275 94-95 0.48466845973195544 96-97 0.4561187832949551 98-99 0.44727826417967265 100-101 0.45793889017163086 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 961482.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.401509068170556 #Duplication Level Percentage of deduplicated Percentage of total 1 69.9026167843155 9.368005527238555 2 9.80944352883947 2.629226928108983 3 4.442183953394703 1.7859590560170253 4 2.6546936968673838 1.4230760660713384 5 1.837577073246038 1.2313152905284543 6 1.3901796075584014 1.1178302770247823 7 1.0154737876371187 0.9526216821455843 8 0.8493133217697648 0.9105664146732448 9 0.7096625841016052 0.8559494600561111 >10 5.67950062325529 15.790062177873418 >50 0.9221520605211055 8.676537067002542 >100 0.7065437570519151 17.833891360962532 >500 0.05196315227494876 4.5722329904578665 >1k 0.013960249864911603 4.1466068459721885 >5k 0.006204555495516269 5.784150168651518 >10k+ 0.00853126380633487 22.92196868721586 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 57251 5.954453645518065 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 29400 3.0577795528153415 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 24771 2.576335282407783 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 18250 1.8981114571047613 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 15552 1.6175029797749725 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 14197 1.4765747044666462 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 14097 1.4661740937427845 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 12655 1.3161972871046987 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12104 1.2588899220162209 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 11408 1.1865016713781433 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10850 1.128466263538995 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 8228 0.8557622503593411 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8135 0.8460896823861497 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7507 0.7807738470402982 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7359 0.7653809431689829 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6817 0.7090096330456525 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6716 0.6985050162145522 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5517 0.5738016936354503 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5371 0.5586168019786122 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4758 0.49486105824133997 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4651 0.4837324047668079 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4015 0.41758452056304746 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3439 0.35767700279360404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2440 0.2537749016622256 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2369 0.2463904680482838 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2218 0.23068554585525264 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2065 0.21477261144774423 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1829 0.19022717013943058 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1816 0.18887509074532857 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1675 0.17421022962468355 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1548 0.16100145400537919 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1477 0.15361702039143738 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1144 0.1189829866809779 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1142 0.11877497446650068 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1121 0.11659084621448973 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1109 0.11534277292762632 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1079 0.11222258971046781 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 993 0.10327806448794674 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 986 0.1025500217372764 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0400610723861705E-4 0.0 12-13 0.0 0.0 0.0 1.0400610723861705E-4 0.0 14-15 0.0 0.0 0.0 2.080122144772341E-4 0.0 16-17 0.0 0.0 0.0 6.240366434317024E-4 0.0 18-19 0.0 0.0 0.0 9.360549651475534E-4 0.0 20-21 0.0 0.0 0.0 0.0017161007694371814 0.0 22-23 0.0 0.0 0.0 0.0035882106997322883 0.0 24-25 0.0 0.0 0.0 0.006500381702413566 0.0 26-27 0.0 0.0 0.0 0.009672567973191385 0.0 28-29 0.0 0.0 0.0 0.0195011451072407 0.0 30-31 0.0 0.0 0.0 0.047530791008047996 0.0 32-33 0.0 0.0 0.0 0.11362667215818914 0.0 34-35 0.0 0.0 0.0 0.19339935641020842 0.0 36-37 0.0 0.0 0.0 0.28658882849600925 0.0 38-39 0.0 0.0 0.0 0.44509413592766167 0.0 40-41 0.0 0.0 0.0 0.6187843350161522 0.0 42-43 0.0 0.0 0.0 0.7858701462949904 0.0 44-45 0.0 0.0 0.0 0.970688998858013 0.0 46-47 0.0 0.0 0.0 1.2193676012655463 0.0 48-49 0.0 0.0 0.0 1.418019266091305 0.0 50-51 0.0 0.0 0.0 1.649796876072563 0.0 52-53 0.0 0.0 0.0 1.8549489226007352 0.0 54-55 0.0 0.0 0.0 2.0778340104130915 0.0 56-57 0.0 0.0 0.0 2.4253704177509303 0.0 58-59 0.0 0.0 0.0 2.698282443145062 0.0 60-61 0.0 0.0 0.0 2.994491836560643 0.0 62-63 0.0 0.0 0.0 3.271252087922603 0.0 64-65 0.0 0.0 0.0 3.5538366812899254 0.0 66-67 0.0 0.0 0.0 3.9135938062283016 0.0 68-69 0.0 0.0 0.0 4.342462989426739 0.0 70-71 0.0 0.0 0.0 4.76810798330078 0.0 72-73 0.0 0.0 0.0 5.118192540265964 0.0 74-75 0.0 0.0 0.0 5.553146080737861 0.0 76-77 0.0 0.0 0.0 5.933392408802245 0.0 78-79 0.0 0.0 0.0 6.603503757740654 0.0 80-81 0.0 0.0 0.0 7.273563103625445 0.0 82-83 0.0 0.0 0.0 7.779812830609414 0.0 84-85 0.0 0.0 0.0 8.339573699767652 0.0 86-87 0.0 0.0 0.0 8.87884536579988 0.0 88-89 0.0 0.0 0.0 9.527011426110942 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACCG 25 0.0050930567 56.79524 5 GCTTTGC 175 0.0 45.98188 1 CTTTGCG 190 0.0 42.345123 2 GGACCGC 45 9.798761E-4 42.06836 6 CCCTTAT 25 0.001603509 38.060474 50-51 GAGATGT 80 1.1047197E-5 35.49518 6 CTCCCGC 230 0.0 34.980755 7 GTATGGT 110 7.2293915E-8 34.42494 1 TACTCCC 265 0.0 33.93426 5 TCCCGCT 245 0.0 32.839073 8 AGTATCG 60 0.004021867 31.55127 9 ACTCCCG 280 0.0 30.424437 6 TTGCGTT 265 0.0 30.362232 4 CCCGCTG 265 0.0 30.360653 9 GGTACTC 285 0.0 29.892231 3 GTAAGCG 160 0.0 29.726196 94-95 CCCTATA 450 0.0 29.44785 2 GACCGCG 100 5.077057E-5 28.396141 7 GTATAGG 740 0.0 28.14471 1 CTATGCG 35 0.008335528 27.18108 66-67 >>END_MODULE