##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936330_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 961482 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.36371143713559 33.0 31.0 34.0 31.0 34.0 2 32.464388308881496 34.0 31.0 34.0 31.0 34.0 3 32.60723341674623 34.0 31.0 34.0 31.0 34.0 4 36.01320045513072 37.0 35.0 37.0 35.0 37.0 5 36.021324372167136 37.0 35.0 37.0 35.0 37.0 6 36.13317357995261 37.0 36.0 37.0 35.0 37.0 7 36.1109069124539 37.0 36.0 37.0 35.0 37.0 8 36.11087779074387 37.0 36.0 37.0 35.0 37.0 9 37.81592167092052 39.0 38.0 39.0 35.0 39.0 10-11 37.747732146831666 39.0 37.5 39.0 35.0 39.0 12-13 37.78001200230477 39.0 38.0 39.0 35.0 39.0 14-15 38.887750888732185 40.0 38.0 41.0 35.0 41.0 16-17 38.92958318512463 40.0 38.0 41.0 35.5 41.0 18-19 39.085854441372796 40.0 38.5 41.0 36.0 41.0 20-21 38.99182720009319 40.0 38.5 41.0 35.0 41.0 22-23 38.88848361175768 40.0 38.0 41.0 35.0 41.0 24-25 38.99316419860175 40.0 38.5 41.0 35.5 41.0 26-27 38.83532817047017 40.0 38.0 41.0 34.5 41.0 28-29 38.85341847273272 40.0 38.0 41.0 35.0 41.0 30-31 38.886875157309234 40.0 38.0 41.0 35.0 41.0 32-33 38.691449241899484 40.0 38.0 41.0 35.0 41.0 34-35 38.4680794856274 40.0 38.0 41.0 34.0 41.0 36-37 38.27233635159057 40.0 37.5 41.0 34.0 41.0 38-39 38.135825215656666 40.0 38.0 41.0 33.5 41.0 40-41 38.26031220553271 40.0 38.0 41.0 34.0 41.0 42-43 38.21808260581061 40.0 38.0 41.0 34.0 41.0 44-45 38.04732069867143 40.0 38.0 41.0 33.0 41.0 46-47 37.86289862940752 40.0 38.0 41.0 33.0 41.0 48-49 37.58777075389867 40.0 38.0 41.0 33.0 41.0 50-51 37.3268111103484 40.0 37.0 41.0 33.0 41.0 52-53 37.39367351650889 40.0 38.0 41.0 32.5 41.0 54-55 37.33531516970676 40.0 37.5 41.0 32.0 41.0 56-57 37.03411244308266 40.0 37.0 41.0 31.5 41.0 58-59 36.86368595563931 40.0 37.0 41.0 31.0 41.0 60-61 36.371660623911836 39.0 36.0 41.0 30.5 41.0 62-63 36.27329008759394 39.0 36.0 41.0 30.5 41.0 64-65 35.956558209098034 39.0 35.5 40.0 30.0 41.0 66-67 35.413740454839505 38.0 35.0 40.0 29.0 41.0 68-69 34.9396577367023 37.0 34.5 39.0 29.0 41.0 70-71 34.34599451679803 36.5 34.0 39.0 28.5 40.5 72-73 33.83023707152084 36.0 34.0 37.5 27.0 40.0 74-75 33.473566847845305 35.0 34.0 37.0 26.5 39.5 76-77 32.75628508906043 35.0 32.5 36.5 26.0 39.0 78-79 32.76151087591863 35.0 33.0 36.0 26.0 39.0 80-81 32.46562910174085 35.0 33.0 36.0 26.0 37.5 82-83 32.10548091383926 35.0 32.5 35.5 25.5 37.0 84-85 31.797986337757752 35.0 32.0 35.0 25.0 37.0 86-87 31.701207614911148 35.0 33.0 35.0 25.0 36.0 88-89 31.134360809666745 34.5 32.0 35.0 23.0 36.0 90-91 30.859986458404837 34.0 32.0 35.0 20.5 36.0 92-93 30.637902737648755 34.0 31.0 35.0 20.0 35.0 94-95 30.45847764180713 34.0 31.0 35.0 19.0 35.0 96-97 30.16945507040173 34.0 31.0 35.0 17.5 35.0 98-99 29.94496204817147 34.0 31.0 35.0 2.0 35.0 100-101 26.563748463309764 31.0 25.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23853043122367268 1101 2 0.1572710661325658 1101 3 0.25451951947830764 1101 4 0.17399294824153344 1101 5 0.22254195842143787 1101 6 0.1865140818751314 1101 7 0.10052458495648864 1101 8 0.11784348968497937 1101 9 0.2661997763795725 1101 10-11 0.17796826743741434 1101 12-13 0.36292096417159314 1101 14-15 0.5753873388384037 1101 16-17 0.5154120820197576 1101 18-19 0.4833581670860667 1101 20-21 0.4883937297246632 1101 22-23 0.5154187822231364 1101 24-25 0.44022276948553696 1101 26-27 0.5146227924071809 1101 28-29 0.5565048859859658 1101 30-31 0.396008592055729 1101 32-33 0.47665670885073297 1101 34-35 0.61370830607747 1101 36-37 0.46898487070269823 1101 38-39 0.5251475378558013 1101 40-41 0.6707329232820811 1101 42-43 0.5060163398636703 1101 44-45 0.4402137734279137 1101 46-47 0.3612861381834378 1101 48-49 0.3580970803988066 1101 50-51 0.5426851606691514 1101 52-53 0.6984859885301518 1101 54-55 0.5459550159024573 1101 56-57 0.55546179680222 1101 58-59 0.2631378981126602 1101 60-61 0.43462715577762623 1101 62-63 0.3933290098082125 1101 64-65 0.41546862629706993 1101 66-67 0.2877163211191558 1101 68-69 0.4856420819296048 1101 70-71 0.5185136904144301 1101 72-73 0.521133156084133 1101 74-75 0.42855599011125634 1101 76-77 0.39805384997453075 1101 78-79 0.3394438810159883 1101 80-81 0.44931513994448125 1101 82-83 0.4900997830778522 1101 84-85 0.8677131137321545 1101 86-87 0.8582346226623514 1101 88-89 0.8524494244276006 1101 90-91 0.9996510592072845 1101 92-93 1.1008549214366994 1101 94-95 1.014237570340061 1101 96-97 1.0225421036397933 1101 98-99 1.1531630280169125 1101 100-101 1.0901319244859664 1102 1 -0.02785333305630644 1102 2 0.05447920317239863 1102 3 0.18381490073722517 1102 4 0.11812572056504678 1102 5 0.059888070311728825 1102 6 0.13873534510621255 1102 7 0.1630358231339244 1102 8 0.1482614899487018 1102 9 0.09908058576809253 1102 10-11 0.153825665454697 1102 12-13 0.11137459095621693 1102 14-15 0.2402588939624124 1102 16-17 0.25359850989763544 1102 18-19 0.2684677318239608 1102 20-21 0.2670495837662159 1102 22-23 0.3306376719511448 1102 24-25 0.2043648919726806 1102 26-27 0.276697090245861 1102 28-29 0.1260766964413662 1102 30-31 0.07768796535501821 1102 32-33 0.18092332037540615 1102 34-35 0.2665879766640131 1102 36-37 0.10063787174559735 1102 38-39 0.17924392845289105 1102 40-41 0.22776592456473566 1102 42-43 0.23172988920975257 1102 44-45 0.2953791394628382 1102 46-47 0.44896190327855123 1102 48-49 0.21892349101483433 1102 50-51 0.25847614854989587 1102 52-53 0.3052906300472671 1102 54-55 0.4031762191965882 1102 56-57 0.338666865070401 1102 58-59 0.16849296458273244 1102 60-61 0.13950062888391557 1102 62-63 0.24339074486293555 1102 64-65 0.23337868491539382 1102 66-67 0.11671921007997099 1102 68-69 0.18736137323515578 1102 70-71 0.1227815006780304 1102 72-73 0.17185509660270526 1102 74-75 0.3545293661196922 1102 76-77 0.04443785400228961 1102 78-79 0.15461482118693226 1102 80-81 0.3125794858525879 1102 82-83 0.2803204709142477 1102 84-85 0.3882295304617607 1102 86-87 0.4129480641219345 1102 88-89 0.44241328244896394 1102 90-91 0.5348909868034504 1102 92-93 0.5068318337249735 1102 94-95 0.7158999215465904 1102 96-97 0.529673101829701 1102 98-99 0.7074688982414372 1102 100-101 1.3487403684029644 1103 1 0.12428041796209044 1103 2 0.15241143262378642 1103 3 0.17734594598261566 1103 4 0.05526641387634612 1103 5 0.18894300048717838 1103 6 0.1872596775559785 1103 7 0.23998339447889805 1103 8 0.19443060649889787 1103 9 0.22595529172469497 1103 10-11 0.24287634993035567 1103 12-13 0.22671418956545608 1103 14-15 0.35332360072226265 1103 16-17 0.4457651937765945 1103 18-19 0.31889832610718116 1103 20-21 0.3114974706699556 1103 22-23 0.2998090146250192 1103 24-25 0.3133359408900276 1103 26-27 0.23265942974119014 1103 28-29 0.3062779655613568 1103 30-31 0.21599880795430693 1103 32-33 0.39928568511545137 1103 34-35 0.3566384086926462 1103 36-37 0.2607853811263041 1103 38-39 0.33249504438344246 1103 40-41 0.3841339005134188 1103 42-43 0.4642364585695731 1103 44-45 0.598536448377665 1103 46-47 0.5022081423917797 1103 48-49 0.5858294086023648 1103 50-51 0.7022780005930045 1103 52-53 0.5355423885702209 1103 54-55 0.6269877101257109 1103 56-57 0.7736531526075581 1103 58-59 0.8116060378956433 1103 60-61 0.7081031051596369 1103 62-63 0.8134132355969825 1103 64-65 0.8079466562741402 1103 66-67 0.7176406416840422 1103 68-69 0.6669662362826472 1103 70-71 0.7225740925056314 1103 72-73 0.5544801439365159 1103 74-75 0.7163131911509666 1103 76-77 0.6881863479263544 1103 78-79 0.5916364627810182 1103 80-81 0.746938958926826 1103 82-83 0.6556632824854987 1103 84-85 0.6253547327062456 1103 86-87 0.7019453904785493 1103 88-89 0.753099536709005 1103 90-91 0.936405095149123 1103 92-93 0.8773265151253646 1103 94-95 0.8507281428278723 1103 96-97 0.6921901706903597 1103 98-99 0.4669586110255821 1103 100-101 0.7344636291004036 1104 1 0.0869681221954508 1104 2 0.07253289110506245 1104 3 0.028266815673227086 1104 4 0.04000928050329122 1104 5 0.1351532727620537 1104 6 0.1051554855514567 1104 7 0.11087934123742116 1104 8 0.1387988673081324 1104 9 0.26478853935144286 1104 10-11 0.08068955309850168 1104 12-13 0.21800340713231492 1104 14-15 0.15046358543111893 1104 16-17 0.2217866666783479 1104 18-19 0.3156625622438014 1104 20-21 0.3236385573206704 1104 22-23 0.21538666343477786 1104 24-25 0.23479469425367228 1104 26-27 0.34406847328286005 1104 28-29 0.3100749035233932 1104 30-31 0.3129275191176575 1104 32-33 0.3159462298828615 1104 34-35 0.2885574472551724 1104 36-37 0.08325030264355604 1104 38-39 0.32821175553591075 1104 40-41 0.4395792618823293 1104 42-43 0.46697566838202675 1104 44-45 0.3917733822040077 1104 46-47 0.5102448356955094 1104 48-49 0.33613550356525934 1104 50-51 0.4815699281205994 1104 52-53 0.5159958444503729 1104 54-55 0.46742244659835563 1104 56-57 0.4029877973877518 1104 58-59 0.42037926370120715 1104 60-61 0.2944137221654586 1104 62-63 0.35136424951954126 1104 64-65 0.42556330566344513 1104 66-67 0.3925671108718447 1104 68-69 0.4582849750081479 1104 70-71 0.3714430376071576 1104 72-73 0.360217315836735 1104 74-75 0.4030066309237128 1104 76-77 0.4065823831123154 1104 78-79 0.4972551419200357 1104 80-81 0.47326353386294784 1104 82-83 0.4784254562222827 1104 84-85 0.5558922626695342 1104 86-87 0.4433529647644008 1104 88-89 0.633004198331232 1104 90-91 0.5289892984723572 1104 92-93 0.7270121215879115 1104 94-95 0.4648478273187884 1104 96-97 0.6799980682124875 1104 98-99 0.5491940837586426 1104 100-101 0.8658042162041468 1105 1 0.07179122583357866 1105 2 0.05797309024556796 1105 3 0.07233239218133036 1105 4 0.04536483449327733 1105 5 -0.011604562263400453 1105 6 0.06493729728122588 1105 7 0.11401961369877966 1105 8 0.07567117159231174 1105 9 0.19356684681373082 1105 10-11 0.1942612183692063 1105 12-13 0.1147749408951384 1105 14-15 0.1443448160734917 1105 16-17 0.21440369955792704 1105 18-19 -0.005195787593244461 1105 20-21 -0.023443742084147345 1105 22-23 -0.05073935973043575 1105 24-25 0.15357422807911547 1105 26-27 0.24545843850111027 1105 28-29 0.14149106594605598 1105 30-31 0.10769825443978931 1105 32-33 0.08224577038806302 1105 34-35 0.07224394708426018 1105 36-37 0.08047924377029148 1105 38-39 0.03491200367876246 1105 40-41 0.22411247414491697 1105 42-43 0.27041574749549824 1105 44-45 0.41301033904908024 1105 46-47 0.3838518045693675 1105 48-49 0.4571873317596129 1105 50-51 0.37896573291489233 1105 52-53 0.4110178537550837 1105 54-55 0.4246099783989905 1105 56-57 0.38682198265208 1105 58-59 0.3264868131406047 1105 60-61 0.47965391974039306 1105 62-63 0.519663675216286 1105 64-65 0.4495608842485339 1105 66-67 0.42779895874019047 1105 68-69 0.4137171114632281 1105 70-71 0.30397806071236033 1105 72-73 0.3903317068169656 1105 74-75 0.3973294827660041 1105 76-77 0.3867590054994352 1105 78-79 0.4034442314628066 1105 80-81 0.4415685695188003 1105 82-83 0.5303149687298827 1105 84-85 0.3464927109444069 1105 86-87 0.410978282118478 1105 88-89 0.49731820470309884 1105 90-91 0.5508968411276207 1105 92-93 0.8304428072814716 1105 94-95 0.4978937480957306 1105 96-97 0.4225276901012407 1105 98-99 0.28349533073513555 1105 100-101 0.4159344194412249 1106 1 -0.14136921523321178 1106 2 -0.0649203522121482 1106 3 -0.06080909994679473 1106 4 0.07778969388812129 1106 5 0.03969868123046183 1106 6 -0.07640616231117292 1106 7 0.03377136244392176 1106 8 0.01286753500876614 1106 9 0.014454019994936118 1106 10-11 0.05683857640335077 1106 12-13 -0.02130464464722337 1106 14-15 -0.04733302173165299 1106 16-17 -0.11238359860563918 1106 18-19 0.03388765829140539 1106 20-21 0.01415049864800011 1106 22-23 0.04051540270229026 1106 24-25 0.11296755966538541 1106 26-27 0.06896494967897127 1106 28-29 0.028213201934924825 1106 30-31 0.02599607531184489 1106 32-33 -0.05647835120768008 1106 34-35 -0.1393453951456678 1106 36-37 -0.051688343465379205 1106 38-39 -0.09723794491720383 1106 40-41 -0.07072494799308515 1106 42-43 -0.15491989062009281 1106 44-45 -0.2726010665993215 1106 46-47 -0.06799974863884728 1106 48-49 -0.11103514692283056 1106 50-51 -0.06581524557604723 1106 52-53 -0.027652595979830608 1106 54-55 0.04612382725097319 1106 56-57 0.07378400414098252 1106 58-59 0.1439268921617156 1106 60-61 -0.20650050403045128 1106 62-63 -0.18187164238002396 1106 64-65 -0.10537339921953048 1106 66-67 -0.12591951849084637 1106 68-69 0.15716813652017692 1106 70-71 -0.10438920600402213 1106 72-73 -0.12271332715002359 1106 74-75 0.024351626818258865 1106 76-77 -0.06754431862820098 1106 78-79 -0.20846285826242905 1106 80-81 -0.1782129129332688 1106 82-83 -0.3028071208945704 1106 84-85 -0.12385043619208247 1106 86-87 -0.1166413078587425 1106 88-89 0.009530442895290037 1106 90-91 0.13292281528449124 1106 92-93 0.30160867097006516 1106 94-95 0.11041063079373714 1106 96-97 0.12052268072756434 1106 98-99 -0.14135650469131633 1106 100-101 0.13747321436936133 1107 1 -0.11530418127560438 1107 2 -0.03533093052044478 1107 3 0.016182050495679334 1107 4 0.14090967742382787 1107 5 0.038896046943570184 1107 6 0.038190462508751466 1107 7 0.09603211568532544 1107 8 0.06171503449425586 1107 9 -0.04988020515427394 1107 10-11 -0.023800443971211394 1107 12-13 0.041666131232588555 1107 14-15 0.1824030350701591 1107 16-17 0.06022136126264854 1107 18-19 -0.03694739007245573 1107 20-21 -0.051338799561499115 1107 22-23 -0.03145928344712701 1107 24-25 -0.021123817161125658 1107 26-27 0.21361148188724144 1107 28-29 0.11896232726936518 1107 30-31 0.014203525404454354 1107 32-33 -0.06429631786748047 1107 34-35 0.1283254218948784 1107 36-37 0.3356058528713177 1107 38-39 0.23872881779116994 1107 40-41 0.22919273054159106 1107 42-43 -0.02801620673452021 1107 44-45 0.10761091117388588 1107 46-47 0.027837872270801256 1107 48-49 -0.13972160504178532 1107 50-51 -0.057819773256632345 1107 52-53 0.013256027193655484 1107 54-55 -0.15277255060455985 1107 56-57 -0.2996827585673927 1107 58-59 -0.2531815663688519 1107 60-61 -0.13322153716483598 1107 62-63 -0.06269845859734602 1107 64-65 -0.0226711971203315 1107 66-67 -0.08176691507450329 1107 68-69 0.09030767140389884 1107 70-71 0.13738379255534738 1107 72-73 0.19597879056968281 1107 74-75 0.06365755396375761 1107 76-77 -5.655164055227146E-4 1107 78-79 -0.20152212971746764 1107 80-81 0.046581283403419604 1107 82-83 0.13150292232420213 1107 84-85 0.06556353787665614 1107 86-87 0.2763819479552154 1107 88-89 0.14317162420831764 1107 90-91 0.08816309106139997 1107 92-93 0.1842079712376119 1107 94-95 0.20724639947042078 1107 96-97 -0.09459102579641154 1107 98-99 -0.13483252856454087 1107 100-101 0.08552836975112754 1108 1 0.001142409185568738 1108 2 0.007357283906046064 1108 3 0.014487135241445515 1108 4 0.027054730636280055 1108 5 -0.09484459160589864 1108 6 -0.09571769673444663 1108 7 -0.12391762810655393 1108 8 -0.09981433089794223 1108 9 -0.032418636372248955 1108 10-11 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-1.8953458954025493 2316 100-101 -1.9924660312731248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 7.0 5 9.0 6 25.0 7 60.0 8 118.0 9 250.0 10 422.0 11 797.0 12 1534.0 13 2272.0 14 2719.0 15 3551.0 16 4839.0 17 6779.0 18 5934.0 19 3425.0 20 2892.0 21 2959.0 22 3151.0 23 3608.0 24 4333.0 25 5130.0 26 6177.0 27 7555.0 28 9778.0 29 12274.0 30 15911.0 31 20069.0 32 25560.0 33 33115.0 34 44873.0 35 67151.0 36 114053.0 37 218444.0 38 281838.0 39 49859.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.950565603663414 20.919148971672367 14.612253581496045 18.518031843168174 2 13.554848864074934 26.897898338373448 34.426451205519264 25.12080159203235 3 26.60403418888757 25.62928895184725 30.81409740379955 16.952579455465624 4 10.497648421915335 16.300253150865018 45.91661622370465 27.285482203514988 5 8.753153985202012 43.579911012374644 36.65497638021304 11.011958622210297 6 25.086377072061673 42.13318606068548 15.943200184714845 16.837236682538 7 23.55998344222773 35.23966127290994 23.908507907584333 17.291847377277993 8 22.72949467592737 27.56401055870001 21.832546007101534 27.873948758271084 9 20.20266630056517 23.022167861696836 27.15131432517717 29.62385151256082 10-11 24.028254401944505 24.93602301039493 28.723315185934993 22.31240740172557 12-13 29.972911595282902 24.527929034516006 26.2571354727054 19.242023897495688 14-15 22.17821030450908 27.940616673011036 32.07189526168976 17.809277760790113 16-17 17.97549295174047 30.568130228601053 30.86569278460205 20.590684035056427 18-19 16.885651525457575 35.42728829036841 29.306684888536655 18.38037529563736 20-21 20.97092922273035 27.903723526517943 26.71961276405878 24.405734486692925 22-23 18.431754312613236 28.710365872684047 29.21105127293075 23.64682854177197 24-25 24.49895081165834 28.758733485359468 27.227308539379013 19.515007163603176 26-27 21.783426837157734 24.640389907827487 35.021862629464835 18.55432062554995 28-29 17.46590088843865 34.652487565811235 29.839920870507626 18.041690675242496 30-31 19.80122073682081 27.51005363150223 28.65933725337856 24.029388378298407 32-33 20.02941349307592 29.433202612475668 30.694768964188444 19.842614930259963 34-35 24.028980804273484 26.857791867402213 29.41269771931138 19.70052960901292 36-37 21.383261863728976 25.254113185803984 33.85355508845208 19.509069862014954 38-39 19.136249631428164 29.957518683406803 32.172745913467914 18.733485771697115 40-41 18.771370104864648 31.18835334160186 30.421980753579774 19.618295799953717 42-43 17.738477163907206 31.30341219169598 26.159356263641513 24.79875438075531 44-45 20.66059700545583 25.750573223630802 28.29418320378054 25.294646567132823 46-47 24.363667948025146 29.706990017678276 28.977806718576332 16.951535315720246 48-49 22.118202645737927 28.53990320978408 29.38740270918987 19.954491435288123 50-51 15.125680695676053 29.601960424349492 35.44142051715969 19.83093836281476 52-53 20.04815818351076 32.92485555145281 26.441131041225695 20.585855223810736 54-55 17.711652868494404 27.600834130715146 29.584130715147484 25.103382285642965 56-57 22.47172371328529 26.105036088701393 28.306501478755276 23.116738719258038 58-59 26.64795576363938 25.79572041396963 27.276887562449847 20.279436259941143 60-61 20.89373563397714 29.097059710651397 32.98891281059211 17.020291844779347 62-63 18.289941985393384 29.399666348407976 31.66252722359857 20.64786444260007 64-65 17.364787105638264 34.579856252037885 28.478498389712886 19.57685825261096 66-67 20.8410557867958 28.14202449967862 25.44571817257109 25.571201540954487 68-69 18.238614971471126 28.898928945107656 28.559765029402527 24.30269105401869 70-71 24.104597025108674 28.351225011843713 26.82846922012459 20.715708742923024 72-73 21.950912562872606 24.432274397067815 33.46263214168334 20.154180898376232 74-75 17.71406016961316 34.646930467757066 27.666352568222806 19.97265679440697 76-77 19.332549679191587 27.8249324984082 27.885321803613355 24.957196018786856 78-79 19.968432401141722 28.846715465260285 29.36525908700715 21.81959304659084 80-81 23.64215208811873 27.49945266416045 27.48171950299306 21.376675744727766 82-83 21.030346901970084 26.018115783758823 32.22353616604367 20.728001148227424 84-85 19.15354013364882 29.401544501937128 30.28690293559375 21.158012428820303 86-87 19.22358819070151 30.984569855299437 28.389050407777344 21.402791546221707 88-89 18.04644005598666 30.858985168556252 25.614420121722866 25.48015465373422 90-91 20.62863050626784 25.80330167420588 27.632534343754696 25.93553347577159 92-93 23.668177385118465 29.673778691046778 27.872964050463906 18.78507987337085 94-95 21.508972992874963 27.534339398534634 29.028512114541456 21.928175494048947 96-97 15.798243287517359 28.789490927812533 33.876520481977835 21.535745302692273 98-99 19.75914529185575 31.59890623651127 25.92248590931831 22.719462562314668 100-101 17.28706696787023 27.762211433254702 28.82677258714726 26.123949011727802 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 946.0 1 553.0 2 293.0 3 410.5 4 282.5 5 260.5 6 575.0 7 991.5 8 1210.5 9 1073.5 10 767.5 11 571.0 12 541.0 13 549.0 14 554.5 15 561.5 16 586.5 17 643.0 18 706.0 19 831.0 20 1025.0 21 1269.0 22 1620.5 23 1999.0 24 2207.0 25 2758.0 26 4416.0 27 6356.5 28 6973.5 29 8063.5 30 11178.0 31 13621.5 32 17504.5 33 22785.5 34 27517.0 35 32180.0 36 38671.0 37 44375.0 38 42986.0 39 39257.0 40 35006.5 41 29154.0 42 23434.0 43 22606.0 44 23814.0 45 22986.0 46 24216.0 47 63972.0 48 143293.5 49 131037.5 50 52231.5 51 18544.0 52 7574.0 53 3798.0 54 2933.5 55 2628.5 56 2591.0 57 2456.5 58 2062.5 59 1720.0 60 1496.5 61 1276.0 62 994.5 63 691.0 64 452.5 65 351.5 66 255.0 67 210.0 68 148.5 69 80.0 70 62.0 71 47.0 72 31.0 73 35.0 74 26.0 75 24.5 76 23.5 77 10.5 78 4.5 79 1.5 80 1.5 81 1.5 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26989584828421126 2 0.020281190911530324 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.6402137533515967E-4 12-13 5.2003053619308534E-5 14-15 0.0 16-17 3.6402137533515967E-4 18-19 0.0 20-21 1.0400610723861707E-4 22-23 0.0 24-25 0.0025481496273461177 26-27 0.002496146573726809 28-29 0.002080122144772341 30-31 0.0014040824477213301 32-33 0.0019241129839144154 34-35 0.0018721099302951068 36-37 1.0400610723861707E-4 38-39 5.2003053619308534E-5 40-41 4.680274825737767E-4 42-43 0.003380198485255054 44-45 0.0034842045924936716 46-47 0.01378080920911676 48-49 0.012948760351207822 50-51 0.00598035116622048 52-53 0.006968409184987343 54-55 2.0801221447723414E-4 56-57 0.003276192378016437 58-59 0.001092064126005479 60-61 0.0012480732868634045 62-63 0.0 64-65 7.800458042896279E-4 66-67 0.0 68-69 0.0 70-71 1.5600916085792556E-4 72-73 0.0014560855013406387 74-75 0.0 76-77 0.19501145107240697 78-79 0.10437012861395221 80-81 6.760396970510108E-4 82-83 0.0 84-85 7.280427506703193E-4 86-87 0.12002304775336407 88-89 0.5761938341019385 90-91 0.30556994306705687 92-93 0.2888249598016395 94-95 0.0014040824477213301 96-97 0.0028081648954426603 98-99 0.0032241893243971287 100-101 6.240366434317022E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 961482.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.871939159017545 #Duplication Level Percentage of deduplicated Percentage of total 1 66.22452870786537 7.862135756543885 2 10.883357431046198 2.584131145344439 3 4.941872999936884 1.7600884676052655 4 2.9975577155904047 1.423472913005316 5 2.086522336180131 1.2385533114530833 6 1.5878475193989914 1.1310497486461055 7 1.1674233452162244 0.9701705249056626 8 0.9213224318627059 0.8750307085529705 9 0.7593020662713221 0.81129491406805 >10 6.528945456986539 16.232237125861353 >50 0.9983900452305637 8.411035567759754 >100 0.8100971858230452 18.309080921915786 >500 0.05867730967583138 4.59671302379849 >1k 0.017515614828606384 4.467787549380129 >5k 0.007006245931442553 5.933275099321088 >10k+ 0.00963358815573351 23.393943221838633 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 58199 6.053051435180274 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 29572 3.0756686032603833 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 24518 2.550021737276413 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 18449 1.918808672445246 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 16364 1.7019559388527294 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 14370 1.494567761018927 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 14173 1.4740785578929196 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 13539 1.4081386859036362 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12766 1.3277419650081854 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 11864 1.2339284562789528 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11188 1.1636203277856476 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8389 0.8725072336247583 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 8010 0.8330889189813225 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7699 0.8007430196301127 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7531 0.783269993614025 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7420 0.7717253157105386 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 7084 0.7367792636783632 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5664 0.589090591399527 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5269 0.5480081790402732 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4939 0.5136861636515296 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4886 0.5081738399678829 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4109 0.4273610946434775 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3363 0.34977253864346913 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2436 0.25335887723327116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2385 0.24805456576410165 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2295 0.23869401611262614 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2111 0.21955689238072057 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1923 0.20000374421986059 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1872 0.19469943275069113 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1826 0.18991515181771473 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1674 0.17410622351744495 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1672 0.1738982113029677 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1124 0.11690286453620556 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1112 0.11565479124934216 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1107 0.11513476071314907 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1073 0.1115985530670361 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1032 0.1073343026702528 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1026 0.10671026602682109 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1006 0.10463014388204875 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 987 0.10265402784451502 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0400610723861705E-4 0.0 12-13 0.0 0.0 0.0 1.0400610723861705E-4 0.0 14-15 0.0 0.0 0.0 2.080122144772341E-4 0.0 16-17 0.0 0.0 0.0 6.240366434317024E-4 0.0 18-19 0.0 0.0 0.0 9.88058018766862E-4 0.0 20-21 0.0 0.0 0.0 0.0018201068766757985 0.0 22-23 0.0 0.0 0.0 0.003692216806970905 0.0 24-25 0.0 0.0 0.0 0.006708393916890799 0.0 26-27 0.0 0.0 0.0 0.009984586294907238 0.0 28-29 0.0 0.0 0.0 0.019865166482575858 0.0 30-31 0.0 0.0 0.0 0.04810282459786039 0.0 32-33 0.0 0.0 0.0 0.11456272712333668 0.0 34-35 0.0 0.0 0.0 0.1958434999303159 0.0 36-37 0.0 0.0 0.0 0.29017703919574156 0.0 38-39 0.0 0.0 0.0 0.44993041991425736 0.0 40-41 0.0 0.0 0.0 0.6256487380939009 0.0 42-43 0.0 0.0 0.0 0.7948146715175115 0.0 44-45 0.0 0.0 0.0 0.9814016279035905 0.0 46-47 0.0 0.0 0.0 1.2321083494022769 0.0 48-49 0.0 0.0 0.0 1.432528118051092 0.0 50-51 0.0 0.0 0.0 1.6673219051422699 0.0 52-53 0.0 0.0 0.0 1.8741900524398791 0.0 54-55 0.0 0.0 0.0 2.099207265450627 0.0 56-57 0.0 0.0 0.0 2.450435889595437 0.0 58-59 0.0 0.0 0.0 2.7249600096517668 0.0 60-61 0.0 0.0 0.0 3.0220014519252567 0.0 62-63 0.0 0.0 0.0 3.29928173382341 0.0 64-65 0.0 0.0 0.0 3.584466479871698 0.0 66-67 0.0 0.0 0.0 3.9481758368851416 0.0 68-69 0.0 0.0 0.0 4.3787091177993975 0.0 70-71 0.0 0.0 0.0 4.805498178853062 0.0 72-73 0.0 0.0 0.0 5.159378958732457 0.0 74-75 0.0 0.0 0.0 5.598752758761995 0.0 76-77 0.0 0.0 0.0 5.9814432303464855 0.0 78-79 0.0 0.0 0.0 6.6526466434109 0.0 80-81 0.0 0.0 0.0 7.324890117547702 0.0 82-83 0.0 0.0 0.0 7.831607872014245 0.0 84-85 0.0 0.0 0.0 8.391680759494196 0.0 86-87 0.0 0.0 0.0 8.930172379722137 0.0 88-89 0.0 0.0 0.0 9.581614632411217 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACGAC 15 6.650324E-4 94.88215 8 GACGACA 15 6.650324E-4 94.88215 9 TCACGCA 25 0.005045754 56.929295 4 GTATGGT 85 1.2732926E-10 50.373188 1 CGGTGCA 20 5.4166105E-4 47.45095 40-41 GACGGTG 20 5.420084E-4 47.44478 38-39 CGCGAAG 20 5.422169E-4 47.44108 30-31 CTCCCGC 205 0.0 43.969776 7 GATGACC 190 0.0 42.44728 9 TTAGGCA 190 0.0 39.950382 4 CATTCCG 155 0.0 39.789295 9 TCCCGCT 230 0.0 39.190456 8 GATCCGG 25 0.0016255552 37.954838 10-11 GGCATTC 175 0.0 37.95286 7 CCCGCTG 240 0.0 37.557518 9 GTATTGA 115 2.4629117E-9 37.232357 1 AGGCATT 180 0.0 36.898613 6 ACTCCCG 250 0.0 36.055218 6 TACTCCC 255 0.0 35.348255 5 CACCGCA 55 0.0025951646 34.5026 9 >>END_MODULE