##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936329_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117530 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19595847868629 33.0 31.0 34.0 30.0 34.0 2 32.25394367395558 33.0 31.0 34.0 30.0 34.0 3 32.34661788479537 34.0 31.0 34.0 30.0 34.0 4 35.83200884880456 37.0 35.0 37.0 35.0 37.0 5 35.83352335573896 37.0 35.0 37.0 35.0 37.0 6 35.970824470348 37.0 35.0 37.0 35.0 37.0 7 35.92942227516379 37.0 35.0 37.0 35.0 37.0 8 35.958861567259426 37.0 35.0 37.0 35.0 37.0 9 37.57002467455118 39.0 37.0 39.0 35.0 39.0 10-11 37.554135114438864 39.0 37.0 39.0 35.0 39.0 12-13 37.54255509231685 39.0 37.0 39.0 35.0 39.0 14-15 38.516089509061516 40.0 38.0 41.0 34.0 41.0 16-17 38.50714285714285 40.0 38.0 41.0 33.5 41.0 18-19 38.60788309367821 40.0 38.0 41.0 34.0 41.0 20-21 38.55027227090956 40.0 38.0 41.0 34.0 41.0 22-23 38.41962477665277 40.0 37.5 41.0 34.0 41.0 24-25 38.429881732323665 40.0 37.5 41.0 34.0 41.0 26-27 38.1699225729601 40.0 37.0 41.0 34.0 41.0 28-29 38.06773589721773 40.0 37.0 41.0 34.0 41.0 30-31 37.91122266655322 40.0 36.5 41.0 33.5 41.0 32-33 37.51524291670212 40.0 35.0 41.0 33.0 41.0 34-35 37.2167148813069 39.0 35.0 41.0 33.0 41.0 36-37 36.934978303411896 39.0 35.0 41.0 32.0 41.0 38-39 36.70021271164809 39.0 35.0 41.0 32.0 41.0 40-41 36.64244448225985 39.0 35.0 41.0 31.5 41.0 42-43 36.45504552029269 39.0 35.0 41.0 31.5 41.0 44-45 36.1076618735642 39.0 35.0 41.0 30.5 41.0 46-47 35.67317706117587 39.0 35.0 41.0 29.0 41.0 48-49 35.197770781928014 39.0 35.0 41.0 27.0 41.0 50-51 34.77674636263082 39.0 35.0 41.0 24.0 41.0 52-53 34.66004849825576 39.0 35.0 41.0 23.5 41.0 54-55 34.42694205734706 38.5 35.0 41.0 22.5 41.0 56-57 33.963345528801156 38.0 34.5 40.5 19.5 41.0 58-59 33.58827107972432 38.0 34.0 40.0 12.0 41.0 60-61 32.966842508295755 37.0 33.0 40.0 4.5 41.0 62-63 32.66759125329703 36.0 33.0 40.0 2.0 41.0 64-65 32.18824980855952 36.0 33.0 39.0 2.0 40.5 66-67 31.584429507359822 35.5 32.0 39.0 2.0 40.0 68-69 31.065944865140814 35.0 32.0 37.5 2.0 39.0 70-71 30.43728409767719 35.0 31.5 37.0 2.0 39.0 72-73 29.926414532459795 35.0 30.5 36.0 2.0 38.0 74-75 29.565115289713265 35.0 31.0 35.5 2.0 37.0 76-77 28.928792648685445 34.0 29.5 35.0 2.0 36.5 78-79 28.971441334127455 34.0 30.0 35.0 2.0 36.0 80-81 28.77462350038288 34.0 30.0 35.0 2.0 36.0 82-83 28.481187781842934 34.0 29.5 35.0 2.0 35.5 84-85 28.247000765761932 34.0 29.0 35.0 2.0 35.0 86-87 28.100340338636943 34.0 29.0 35.0 2.0 35.0 88-89 27.626644261039736 34.0 28.0 35.0 2.0 35.0 90-91 27.388028588445504 34.0 27.0 35.0 2.0 35.0 92-93 27.09771973113248 34.0 27.0 35.0 2.0 35.0 94-95 26.91425168042202 34.0 26.0 35.0 2.0 35.0 96-97 26.63722028418276 34.0 25.0 35.0 2.0 35.0 98-99 26.366510678124733 34.0 25.0 35.0 2.0 35.0 100-101 23.47265804475453 29.5 12.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.6132841367487103 1101 2 -0.17940382286043643 1101 3 -0.13420879480617742 1101 4 0.23105408284228446 1101 5 0.006865102312190174 1101 6 0.013244745729913632 1101 7 -0.21335817345202912 1101 8 -0.039407064384192836 1101 9 -0.17008728124515216 1101 10-11 0.15996864644133524 1101 12-13 0.29145727208150873 1101 14-15 0.01182805891312455 1101 16-17 0.057478920584728144 1101 18-19 0.0234788088083846 1101 20-21 0.36885396684709804 1101 22-23 0.18940631152132426 1101 24-25 0.18045774592329877 1101 26-27 0.5118960920995121 1101 28-29 0.2903391559280948 1101 30-31 0.03159790312624722 1101 32-33 1.012415484366329 1101 34-35 0.9787207689260526 1101 36-37 0.34104265320474525 1101 38-39 0.7417032512135151 1101 40-41 1.4879724326773243 1101 42-43 1.193928971160929 1101 44-45 0.725800683388087 1101 46-47 0.5652850127991371 1101 48-49 0.13661600288564557 1101 50-51 0.4511228222986574 1101 52-53 0.329002641775368 1101 54-55 0.571299079610526 1101 56-57 0.3365799568136225 1101 58-59 0.32906192425353 1101 60-61 -0.4195477622514332 1101 62-63 -0.567645195894471 1101 64-65 0.05410122288008523 1101 66-67 0.18356563838259987 1101 68-69 0.0943924075141318 1101 70-71 0.4364559382235704 1101 72-73 0.4383126564905666 1101 74-75 0.34240632146530103 1101 76-77 0.6767996838840453 1101 78-79 0.19106438824374905 1101 80-81 0.5669354484664488 1101 82-83 1.1137648994025007 1101 84-85 1.1014321273851913 1101 86-87 0.5059326558455659 1101 88-89 -0.025812884951406545 1101 90-91 0.6634390330674655 1101 92-93 0.7455965913874962 1101 94-95 1.110326635373422 1101 96-97 1.5282905478193918 1101 98-99 1.4861261298492323 1101 100-101 1.870413295786559 1102 1 0.13949107139190176 1102 2 0.07337138528017562 1102 3 0.20066632082749436 1102 4 0.11264520217623186 1102 5 0.15811394597546524 1102 6 -0.12574692864566117 1102 7 -0.1650232983363935 1102 8 0.07877054893680224 1102 9 -0.47744158281777516 1102 10-11 -0.06343560887781763 1102 12-13 0.15558770686411805 1102 14-15 0.17487153717399906 1102 16-17 -0.06682727738012062 1102 18-19 0.216355774580812 1102 20-21 0.23703158016809311 1102 22-23 0.0957430367757155 1102 24-25 0.008047940187871916 1102 26-27 0.054333649916344484 1102 28-29 -0.3032084851449852 1102 30-31 -0.13815232814109635 1102 32-33 -0.11732549620720789 1102 34-35 0.197152776326611 1102 36-37 0.2600990824369447 1102 38-39 0.5290529274392313 1102 40-41 -0.04891470885829108 1102 42-43 -0.1155067365171476 1102 44-45 -0.21406980689869215 1102 46-47 0.22219990641615794 1102 48-49 0.08909056347768995 1102 50-51 0.027556679837971387 1102 52-53 0.06905814593817894 1102 54-55 -0.08307187321093323 1102 56-57 -0.2502609312529813 1102 58-59 0.3457131730971881 1102 60-61 0.21227462442757172 1102 62-63 0.18651761631645059 1102 64-65 0.6668440535923921 1102 66-67 0.67315860785531 1102 68-69 0.42082626505344933 1102 70-71 0.35424039705798194 1102 72-73 0.43195280450166607 1102 74-75 0.49932121508232186 1102 76-77 -0.1437368563564725 1102 78-79 -0.10958316032239424 1102 80-81 0.3207513596597842 1102 82-83 0.08011550069759465 1102 84-85 0.328652293897683 1102 86-87 -0.6611580009536944 1102 88-89 -0.23568800058507833 1102 90-91 0.04549268709152088 1102 92-93 -0.1339131218409939 1102 94-95 -0.7349555107875396 1102 96-97 -0.985354225538611 1102 98-99 -1.5790912614551154 1102 100-101 -0.7004701454715345 1103 1 -0.3843645056551672 1103 2 -0.3090700503527515 1103 3 -0.21326017820538823 1103 4 0.09512962083086052 1103 5 -0.13696449803914845 1103 6 0.18265625209794223 1103 7 0.15939105799285613 1103 8 0.08564783240983331 1103 9 0.5458767064579604 1103 10-11 0.21299894947163978 1103 12-13 0.15306245433886545 1103 14-15 0.20769982000228282 1103 16-17 0.24791589906517686 1103 18-19 0.4346557960723274 1103 20-21 0.7312826010148541 1103 22-23 0.7998586611041034 1103 24-25 0.8448736440348412 1103 26-27 0.7564613094908168 1103 28-29 0.5956202275135212 1103 30-31 0.32606430628830196 1103 32-33 0.3438823267027402 1103 34-35 0.7399746117016335 1103 36-37 1.0568968473198126 1103 38-39 0.605723892408065 1103 40-41 0.5825596087862408 1103 42-43 0.5267133365539962 1103 44-45 0.8220896601118426 1103 46-47 0.24079006330418906 1103 48-49 -0.054150356358981355 1103 50-51 -0.1686070854747257 1103 52-53 -0.31263108681488205 1103 54-55 -0.5780750969375603 1103 56-57 -0.4368609742360121 1103 58-59 -0.8479253396902564 1103 60-61 -0.30975417419697493 1103 62-63 -0.24067989066828943 1103 64-65 0.06261022595430887 1103 66-67 -0.19950419033940392 1103 68-69 -0.010185985107735718 1103 70-71 -0.3039371228216652 1103 72-73 -0.3934286698159788 1103 74-75 -0.36845226439328727 1103 76-77 0.03834996934737589 1103 78-79 -0.3459360509306002 1103 80-81 -0.33705005877993344 1103 82-83 -0.18745380942490542 1103 84-85 -0.2924523697601771 1103 86-87 -0.48874235513379105 1103 88-89 0.07470314491244778 1103 90-91 0.30935471159184047 1103 92-93 0.7192461302544295 1103 94-95 1.0941869779294144 1103 96-97 1.2329457959529044 1103 98-99 1.0976764153125629 1103 100-101 0.9808107485753155 1104 1 0.1652088982871689 1104 2 0.1864678529521413 1104 3 0.2942420283259466 1104 4 0.3211764973631759 1104 5 0.24177434671911158 1104 6 0.3495680889126902 1104 7 0.47585668513809054 1104 8 0.41299901412994444 1104 9 0.43280423416219094 1104 10-11 0.18181330663551165 1104 12-13 0.041510036961206254 1104 14-15 0.5510851294070136 1104 16-17 0.40198082012040004 1104 18-19 0.5134534816661258 1104 20-21 0.41834742400201463 1104 22-23 0.551795505273958 1104 24-25 0.4072939637367199 1104 26-27 0.21277198910246398 1104 28-29 0.21398837500787238 1104 30-31 -0.1068571411962509 1104 32-33 -0.06113875792999579 1104 34-35 0.26929696141232995 1104 36-37 0.4153356038168212 1104 38-39 0.8830603639192987 1104 40-41 0.7785486251636016 1104 42-43 0.9344312928156384 1104 44-45 0.6416934651525423 1104 46-47 0.8262073428962253 1104 48-49 0.9053475351777465 1104 50-51 1.5774947091707574 1104 52-53 1.4222909490780253 1104 54-55 1.5051846853513453 1104 56-57 1.3569999652560014 1104 58-59 1.7767191635950255 1104 60-61 1.5916383922441426 1104 62-63 1.8367448420962447 1104 64-65 1.6952472760670858 1104 66-67 1.9080274366096965 1104 68-69 1.85668662448332 1104 70-71 2.5601895811108655 1104 72-73 2.0447394188375476 1104 74-75 1.503814088455595 1104 76-77 1.5169220984049403 1104 78-79 1.9028626153437926 1104 80-81 1.6322056046504905 1104 82-83 1.8210781117283723 1104 84-85 1.9239838369672952 1104 86-87 1.8274290678337444 1104 88-89 1.9984061948290908 1104 90-91 2.3602520343338256 1104 92-93 2.4062128851823488 1104 94-95 2.6927453774586887 1104 96-97 2.748636685430199 1104 98-99 1.8820737870885758 1104 100-101 0.8228721391803973 1105 1 0.0600799387892792 1105 2 0.014162272879573834 1105 3 -0.2536642186094298 1105 4 -0.5210319953307518 1105 5 0.2266718655972184 1105 6 0.08164615108784545 1105 7 -0.27495237635058345 1105 8 -0.2476855009235024 1105 9 -0.5551333945521364 1105 10-11 -0.6657889293162427 1105 12-13 -0.17522037394396506 1105 14-15 -0.14078502848256846 1105 16-17 0.27316842431770993 1105 18-19 0.2730396344561612 1105 20-21 -0.02629315656089659 1105 22-23 -0.11428275303731539 1105 24-25 -0.40765160849040427 1105 26-27 -0.3293972763677715 1105 28-29 0.13602426791755562 1105 30-31 -0.2143397341157396 1105 32-33 0.6216601044805259 1105 34-35 0.3813887531157789 1105 36-37 0.3750786655016256 1105 38-39 0.7168350035191793 1105 40-41 0.8704383966650298 1105 42-43 0.001460811301470244 1105 44-45 0.5291603671825484 1105 46-47 0.9175577400718637 1105 48-49 0.8700920678834478 1105 50-51 0.4374535205858763 1105 52-53 0.8035305293467374 1105 54-55 0.36131884245637735 1105 56-57 0.5631390257639879 1105 58-59 0.3338928421279235 1105 60-61 0.37711451267171725 1105 62-63 0.4158857658973574 1105 64-65 0.34543850878259974 1105 66-67 0.28029378945857886 1105 68-69 0.3837534088316552 1105 70-71 0.3374770185924767 1105 72-73 0.17727840089109392 1105 74-75 -0.1260280219690415 1105 76-77 -0.1788217478243439 1105 78-79 -0.25502696002151026 1105 80-81 -0.02391874564861851 1105 82-83 -0.10664572861682586 1105 84-85 -0.5550786323864365 1105 86-87 -0.2463181127095524 1105 88-89 -0.05155948135017496 1105 90-91 -0.19064528840815953 1105 92-93 -0.29590538489708607 1105 94-95 -0.7340958503534125 1105 96-97 -0.1963471333400264 1105 98-99 -0.6952528776167313 1105 100-101 -0.18080541304084363 1106 1 0.41420946938939807 1106 2 0.30887409700316226 1106 3 0.2029608556627096 1106 4 0.16583669153793323 1106 5 0.44889408781943985 1106 6 0.4610282069404832 1106 7 0.22504762364941655 1106 8 0.15219566366354798 1106 9 0.49210368209670463 1106 10-11 0.5031059013432966 1106 12-13 0.3516661382366735 1106 14-15 0.8366362430563541 1106 16-17 0.6611660476094485 1106 18-19 0.4220295334460573 1106 20-21 0.2712410086203221 1106 22-23 0.37056573181116903 1106 24-25 0.5968047194953314 1106 26-27 0.14861817223591345 1106 28-29 0.36909022157174576 1106 30-31 0.6464742884629899 1106 32-33 -0.0020772692568584716 1106 34-35 0.5685545285168487 1106 36-37 0.8847340428040695 1106 38-39 0.8500792102927974 1106 40-41 0.5631003099151783 1106 42-43 0.7239801220560764 1106 44-45 0.7185543065764932 1106 46-47 0.4647948167207048 1106 48-49 0.015665448750944222 1106 50-51 -0.03491724590253398 1106 52-53 -0.12368278021951795 1106 54-55 0.26194272837437893 1106 56-57 0.8089797863106369 1106 58-59 0.8126510120625667 1106 60-61 0.9868887253871037 1106 62-63 0.9809481544891625 1106 64-65 0.8334064232210991 1106 66-67 0.8660038310509712 1106 68-69 1.3877641040188244 1106 70-71 1.4938941308919418 1106 72-73 1.5658702012475985 1106 74-75 1.4723082822496139 1106 76-77 1.6802097435600913 1106 78-79 1.4527864683801504 1106 80-81 1.4717734706318346 1106 82-83 1.718517670075986 1106 84-85 1.7268583848446966 1106 86-87 1.3438482568685828 1106 88-89 1.5022135430110808 1106 90-91 1.7719215565116286 1106 92-93 2.241973402981703 1106 94-95 2.5046147854160488 1106 96-97 1.583064631365854 1106 98-99 1.0042420718782203 1106 100-101 1.2594797919502412 1107 1 -0.05463153235783835 1107 2 -0.10094923827154645 1107 3 0.07026817462989499 1107 4 -0.002480140145237897 1107 5 -0.17156795838518235 1107 6 0.12915090156288755 1107 7 -0.010925973710314452 1107 8 -0.19298003037644662 1107 9 0.5525773765249724 1107 10-11 0.1376914187185605 1107 12-13 0.04420156825025856 1107 14-15 0.07833688370865843 1107 16-17 0.45023613108838845 1107 18-19 0.2396862991226314 1107 20-21 0.07666713946871084 1107 22-23 -0.1607874035023542 1107 24-25 0.43363251992243335 1107 26-27 0.5831939827581465 1107 28-29 0.7153822037111297 1107 30-31 0.5186835682144917 1107 32-33 0.48389632810288674 1107 34-35 0.3786884830887729 1107 36-37 0.8040215597910603 1107 38-39 0.6035736773865636 1107 40-41 0.6206134141667761 1107 42-43 0.3608467498953303 1107 44-45 0.3464420953553642 1107 46-47 0.7694929335912164 1107 48-49 0.8905441130879694 1107 50-51 0.8306428395177718 1107 52-53 1.4878489611899184 1107 54-55 2.048537564328562 1107 56-57 1.91842455431685 1107 58-59 1.869096362479958 1107 60-61 2.043431144334882 1107 62-63 2.098304007721545 1107 64-65 1.9891528802197413 1107 66-67 1.7340391639960266 1107 68-69 1.7263876722579958 1107 70-71 1.3470779786884854 1107 72-73 1.7705877318241825 1107 74-75 1.291663747207874 1107 76-77 1.2328694212925697 1107 78-79 1.7512236650409925 1107 80-81 1.545788225048451 1107 82-83 2.326797615717606 1107 84-85 2.451572032680069 1107 86-87 1.9298995090526248 1107 88-89 2.218967571355094 1107 90-91 2.4887726533860217 1107 92-93 2.470521257767178 1107 94-95 2.8325608153131547 1107 96-97 2.9241149128645922 1107 98-99 2.856552302901317 1107 100-101 2.6849311606165216 1108 1 0.15311555814068356 1108 2 0.07822394220439577 1108 3 -0.052973096865670755 1108 4 -0.01837383477785437 1108 5 0.24713970185452183 1108 6 -0.3614692130801558 1108 7 0.20750376400029324 1108 8 -0.3359364312796984 1108 9 -0.12451138498276038 1108 10-11 0.13365285696765028 1108 12-13 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-2.8472335972491187 2316 96-97 -3.378937366885676 2316 98-99 -3.1841868028563916 2316 100-101 -2.758879143980817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 11.0 6 18.0 7 52.0 8 76.0 9 115.0 10 178.0 11 297.0 12 542.0 13 745.0 14 886.0 15 1160.0 16 1499.0 17 1858.0 18 1714.0 19 1096.0 20 1140.0 21 1263.0 22 1213.0 23 1081.0 24 1117.0 25 1260.0 26 1339.0 27 1305.0 28 1685.0 29 1943.0 30 2348.0 31 3000.0 32 3646.0 33 4658.0 34 6486.0 35 11769.0 36 14800.0 37 25526.0 38 20023.0 39 1678.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.01492448212047 19.621856264444766 13.570191971481446 16.793027281953314 2 15.085524636201175 26.657305761211813 31.991319887669135 26.265849714917877 3 28.51442184974049 26.133753084318894 28.73308942397686 16.618735641963752 4 12.201140134433762 18.13579511614056 37.49425678550157 32.16880796392411 5 10.368416574491619 42.416404322300686 32.44192972007147 14.77324938313622 6 28.37147962222411 36.50897643154939 13.764996171190337 21.354547775036163 7 22.010550497745257 37.12328767123287 20.83042627414277 20.035735556879093 8 25.65898068578235 26.62298987492555 19.456309027482344 28.261720411809748 9 21.363056240959754 22.62911597039054 24.455032757593806 31.5527950310559 10-11 25.79937037352165 25.959754956181403 23.550157406619586 24.69071726367736 12-13 31.39368671828469 25.071896537054368 24.058112822258146 19.476303922402792 14-15 24.311239683485066 27.292606143112398 30.00127626988854 18.394877903513994 16-17 18.412824014396385 30.912239054875585 29.176504622243776 21.498432308484254 18-19 18.02135624946822 32.658470177826935 29.945971241385177 19.374202331319662 20-21 21.591083127712075 26.690206755721945 27.781417510422873 23.937292606143114 22-23 18.735641963753935 27.71505147621884 30.25525397770782 23.29405258231941 24-25 23.72370839289361 27.778486828670612 28.08309509223334 20.414709686202436 26-27 21.618919585804356 24.80111291681202 34.72036688817228 18.859600609211345 28-29 18.08547750323327 32.57861956299775 30.24555850520727 19.090344428561703 30-31 19.743128320974733 27.37270217264602 30.000127627531807 22.884041878847437 32-33 19.43647950718546 28.781832568982974 31.755992138110596 20.02569578572097 34-35 22.15103442114533 26.91026508238357 30.6574094163593 20.281291080111803 36-37 20.441163958138347 26.11248191950991 34.200629626478346 19.245724495873397 38-39 19.49255293351882 28.61579433248674 31.93070675872866 19.960945975265783 40-41 18.906236705521994 29.809410363311496 30.72109248702459 20.56326044414192 42-43 18.66244070536683 29.66497202782328 28.156814362595988 23.515772904213907 44-45 20.52200548807726 25.858203399204445 29.402054838229354 24.21773627448895 46-47 22.971144600355693 29.530195631270367 29.86163703974744 17.637022728626498 48-49 21.428662601954635 27.51637018095485 30.28706851436619 20.76789870272432 50-51 15.645740444504952 29.115329634797998 34.390422381811376 20.848507538885674 52-53 19.78736348591145 31.10457819433395 27.26154971942021 21.8465086003344 54-55 17.742779472387785 26.875380223688524 29.495573451771683 25.886266852152012 56-57 21.58099840887626 26.008917099900447 28.030154772945792 24.379929718277502 58-59 25.015315238662467 25.752573810941886 27.750787033097936 21.48132391729771 60-61 20.429252105845315 28.233642474261888 30.968688845401175 20.36841657449162 62-63 17.58487194758785 28.978558665872544 30.22122011401344 23.215349272526165 64-65 17.393579456985083 32.36860689702116 27.971819721090114 22.26599392490364 66-67 20.115289713264698 26.93652684420999 25.841061856547263 27.10712158597805 68-69 18.705436909725176 27.156470688334895 28.149834084914488 25.98825831702544 70-71 23.02868641490009 27.26081536975823 26.008363857584694 23.70213435775699 72-73 21.338926374365037 24.231878632144102 31.674423749436297 22.75477124405456 74-75 18.19407810771718 31.07844805581554 27.433846677444055 23.293627159023227 76-77 19.291202679724357 26.142228245351607 27.61548001073935 26.95108906418468 78-79 20.40399223353885 26.892223319821507 28.800200974213986 23.90358347242566 80-81 22.223025806396716 25.27886734337908 27.725922963694067 24.772183886530133 82-83 19.66008678635242 24.63498681187782 31.297115630051902 24.40781077171786 84-85 19.24019399302306 27.012677614226156 29.27890751297541 24.468220879775377 86-87 18.88328116016099 27.78466321684874 28.11942332673183 25.212632296258437 88-89 18.3912915110204 27.917912906552335 25.274386721608195 28.416408860819075 90-91 20.06793866742913 24.201870020355994 27.096518143294258 28.63367316892062 92-93 21.947899554095283 26.54050478974206 27.651212903501097 23.86038275266156 94-95 20.160130691148566 25.16144951458789 28.458933539807198 26.21948625445635 96-97 15.980940630916168 26.41126544851205 31.834251558145965 25.773542362425815 98-99 19.513056352049283 27.727199244441795 25.825966357239487 26.93377804626943 100-101 17.457244958733938 25.51476218837744 27.14115544967242 29.886837403216198 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2708.0 1 1522.0 2 488.0 3 636.5 4 423.5 5 283.5 6 579.0 7 976.5 8 1155.5 9 1009.5 10 595.5 11 277.5 12 209.5 13 204.0 14 191.5 15 169.0 16 167.0 17 176.0 18 187.0 19 201.0 20 219.0 21 241.5 22 272.5 23 313.0 24 347.5 25 382.5 26 457.5 27 543.0 28 621.5 29 709.5 30 814.5 31 987.0 32 1165.5 33 1282.0 34 1428.5 35 1421.0 36 1399.5 37 1505.0 38 1461.5 39 1450.5 40 1481.0 41 1445.5 42 1342.5 43 1246.5 44 1386.5 45 1653.5 46 2432.5 47 6971.0 48 14823.0 49 14784.5 50 9169.0 51 6059.5 52 3512.0 53 2129.0 54 1801.0 55 1819.0 56 1855.0 57 1873.5 58 1936.5 59 2023.0 60 1945.0 61 1961.0 62 1785.0 63 1258.5 64 821.5 65 602.0 66 571.5 67 424.5 68 178.0 69 74.5 70 63.0 71 48.5 72 50.0 73 65.0 74 55.5 75 25.5 76 16.5 77 9.0 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23228111971411555 2 0.017016931847187954 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.254232961796988E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017016931847187952 26-27 8.508465923593976E-4 28-29 0.0017016931847187952 30-31 4.254232961796988E-4 32-33 8.508465923593976E-4 34-35 4.254232961796988E-4 36-37 0.0 38-39 4.254232961796988E-4 40-41 0.0 42-43 0.0021271164808984943 44-45 0.0021271164808984943 46-47 0.011061005700672168 48-49 0.012337275589211265 50-51 0.005105079554156385 52-53 0.004679656257976687 54-55 4.254232961796988E-4 56-57 0.0025525397770781925 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.508465923593976E-4 66-67 0.0 68-69 0.0 70-71 4.254232961796988E-4 72-73 0.0025525397770781925 74-75 0.0 76-77 0.1739981281374968 78-79 0.08678635242065856 80-81 8.508465923593976E-4 82-83 0.0 84-85 0.0 86-87 0.11273717348762019 88-89 0.5772994129158513 90-91 0.3109844295073598 92-93 0.29992342380668763 94-95 8.508465923593976E-4 96-97 0.0021271164808984943 98-99 8.508465923593976E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 117530.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.467370033183016 #Duplication Level Percentage of deduplicated Percentage of total 1 71.66863973666734 21.11886326895261 2 11.327346750209339 6.675742363651834 3 5.512083850662663 4.87279843444227 4 3.0173533912742183 3.5565387560622823 5 1.9114717177258682 2.816302220709606 6 1.354199751681922 2.394282310899345 7 1.030808766205642 2.1262656343061344 8 0.7189674587820865 1.6948864119799198 9 0.6352322928998354 1.6846762528716073 >10 2.56402852770479 12.766102271760401 >50 0.08662258539543209 1.761252446183953 >100 0.09528484393497531 6.085254828554412 >500 0.037536453671353914 8.4744320598996 >1k 0.037536453671353914 19.255509231685526 >5k 0.00288741951318107 4.7170935080405005 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5544 4.7170935080405005 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3225 2.743980260359057 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2938 2.4997872883519103 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2350 1.9994894920445843 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1607 1.3673104739215518 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1602 1.3630562409597549 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1555 1.3230664511188632 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1477 1.2567004169148304 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1365 1.1614055985705778 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1343 1.1426869735386709 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1327 1.1290734280609207 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1310 1.114609035990811 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1302 1.1078022632519355 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1230 1.046541308602059 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 992 0.8440398196205224 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 990 0.8423381264358036 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 928 0.789585637709521 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 928 0.789585637709521 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 884 0.7521483876457075 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 858 0.7300263762443632 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 840 0.714711137581894 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 809 0.6883348932187527 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 565 0.48072832468305965 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 559 0.4756232451289032 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 548 0.4662639326129499 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 547 0.4654130860205905 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 512 0.43563345528801156 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 494 0.4203182166255424 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 482 0.4101080575172296 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 458 0.3896877393006041 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 444 0.37777588700757253 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 363 0.3088573130264613 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 338 0.2875861482174764 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 293 0.2492980515613035 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 279 0.23738619926827192 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 243 0.20675572194333364 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 241 0.2050540287586148 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 227 0.19314217646558327 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 225 0.19144048328086444 TruSeq Adapter, Index 11 (95% over 21bp) GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 191 0.16251169914064495 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 185 0.15740661958648855 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 180 0.15315238662469158 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 179 0.15230154003233218 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 174 0.14804730707053518 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 173 0.14719646047817578 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 168 0.14294222751637878 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 164 0.1395388411469412 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 162 0.1378371479622224 TruSeq Adapter, Index 11 (96% over 28bp) GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 155 0.13188122181570663 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 149 0.12677614226155023 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 143 0.12167106270739385 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 129 0.10975921041436229 RNA PCR Primer, Index 11 (95% over 23bp) GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 126 0.1072066706372841 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.254232961796988E-4 0.0 16-17 0.0 0.0 0.0 0.002127116480898494 0.0 18-19 0.0 0.0 0.0 0.0029779630732578916 0.0 20-21 0.0 0.0 0.0 0.007232196035054879 0.0 22-23 0.0 0.0 0.0 0.01744235514336765 0.0 24-25 0.0 0.0 0.0 0.0331830171020165 0.0 26-27 0.0 0.0 0.0 0.05870841487279843 0.0 28-29 0.0 0.0 0.0 0.12975410533480813 0.0 30-31 0.0 0.0 0.0 0.27609971922062454 0.0 32-33 0.0 0.0 0.0 0.529652003743725 0.0 34-35 0.0 0.0 0.0 0.8874329958308517 0.0 36-37 0.0 0.0 0.0 1.3558240449247 0.0 38-39 0.0 0.0 0.0 1.8880285884455033 0.0 40-41 0.0 0.0 0.0 2.5031906747213477 0.0 42-43 0.0 0.0 0.0 3.0562409597549562 0.0 44-45 0.0 0.0 0.0 3.542499787288352 0.0 46-47 0.0 0.0 0.0 4.141495788309368 0.0 48-49 0.0 0.0 0.0 4.752829064919595 0.0 50-51 0.0 0.0 0.0 5.263762443631413 0.0 52-53 0.0 0.0 0.0 5.637284097677189 0.0 54-55 0.0 0.0 0.0 6.03080064664341 0.0 56-57 0.0 0.0 0.0 6.531523866246916 0.0 58-59 0.0 0.0 0.0 6.908874329958309 0.0 60-61 0.0 0.0 0.0 7.324087467029695 0.0 62-63 0.0 0.0 0.0 7.705266740406705 0.0 64-65 0.0 0.0 0.0 8.053688419977878 0.0 66-67 0.0 0.0 0.0 8.47187952012252 0.0 68-69 0.0 0.0 0.0 8.971751893133668 0.0 70-71 0.0 0.0 0.0 9.479707308772227 0.0 72-73 0.0 0.0 0.0 9.920020420318217 0.0 74-75 0.0 0.0 0.0 10.486258827533396 0.0 76-77 0.0 0.0 0.0 10.956351569811963 0.0 78-79 0.0 0.0 0.0 11.712328767123289 0.0 80-81 0.0 0.0 0.0 12.447885646217987 0.0 82-83 0.0 0.0 0.0 13.010295243767548 0.0 84-85 0.0 0.0 0.0 13.673955585807878 0.0 86-87 0.0 0.0 0.0 14.306560027227091 0.0 88-89 0.0 0.0 0.0 14.985110184633712 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGGG 55 1.5097612E-10 68.998375 5 CGCAGAA 80 2.2632048E-7 41.506836 9 CTGGGAA 75 6.932889E-6 37.94911 4 TGGGGGA 65 1.3313959E-4 36.489525 6 CATGGGG 225 0.0 31.624254 4 GTACTGG 65 0.005788456 29.266342 1 ACTGGGA 65 0.0058612814 29.19162 3 TACTACA 35 0.008071731 27.350916 84-85 CTGGAAT 35 0.008276741 27.210716 72-73 GAGTACT 980 0.0 23.960213 12-13 AGTACTT 960 0.0 21.741676 12-13 GTAATGG 110 0.0028420128 21.617186 1 GTACTTT 1075 0.0 21.180897 14-15 ACTTTTT 1140 0.0 20.181269 16-17 TACTTTT 1070 0.0 19.50655 14-15 GTGTACA 65 0.007585585 18.25253 68-69 ACCCCCA 65 0.0076042353 18.244762 94-95 TTTTGGG 80 0.0011944927 17.796217 42-43 TACATGG 1325 0.0 16.117329 2 GTACATG 1340 0.0 15.970905 1 >>END_MODULE