##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936327_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 964219 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.731910489214588 33.0 31.0 34.0 30.0 34.0 2 31.846168764564897 33.0 31.0 34.0 30.0 34.0 3 31.907676575549747 34.0 31.0 34.0 30.0 34.0 4 35.42316112833288 37.0 35.0 37.0 33.0 37.0 5 35.44318562484249 37.0 35.0 37.0 33.0 37.0 6 35.55766169303862 37.0 35.0 37.0 33.0 37.0 7 35.5048666329952 37.0 35.0 37.0 33.0 37.0 8 35.50906277515793 37.0 35.0 37.0 33.0 37.0 9 37.17233844178553 39.0 37.0 39.0 34.0 39.0 10-11 37.098426291122664 39.0 37.0 39.0 34.0 39.0 12-13 37.079110139916345 39.0 37.0 39.0 33.5 39.0 14-15 38.246410307202 40.0 38.0 41.0 33.0 41.0 16-17 38.236139818858575 40.0 38.0 41.0 33.0 41.0 18-19 38.28406150469966 40.0 38.0 41.0 33.5 41.0 20-21 38.209280256871104 40.0 38.0 41.0 34.0 41.0 22-23 38.04516193935196 40.0 38.0 41.0 33.0 41.0 24-25 38.218613198868724 40.0 38.0 41.0 34.0 41.0 26-27 38.15334638707596 40.0 38.0 41.0 34.0 41.0 28-29 38.07116277526164 40.0 38.0 41.0 33.0 41.0 30-31 37.97069493548665 40.0 38.0 41.0 33.0 41.0 32-33 37.77362144906914 40.0 38.0 41.0 33.0 41.0 34-35 37.524665558343074 40.0 37.0 41.0 32.5 41.0 36-37 37.334894354913146 40.0 37.0 41.0 32.0 41.0 38-39 37.188622605445445 40.0 37.0 41.0 32.0 41.0 40-41 37.13684183779826 40.0 37.0 41.0 31.5 41.0 42-43 36.98217313701555 40.0 37.0 41.0 31.0 41.0 44-45 36.818131565546835 40.0 37.0 41.0 31.0 41.0 46-47 36.55093293121169 40.0 37.0 41.0 30.0 41.0 48-49 36.36822651285652 40.0 36.5 41.0 30.0 41.0 50-51 36.042113358064924 39.5 36.5 40.5 29.5 41.0 52-53 36.18877194911114 39.5 37.0 40.5 30.0 41.0 54-55 36.37307603355669 40.0 37.0 41.0 30.0 41.0 56-57 36.26063269858818 40.0 36.5 41.0 29.0 41.0 58-59 36.11858198189415 40.0 36.0 41.0 28.5 41.0 60-61 35.856195013788366 39.5 36.0 41.0 28.0 41.0 62-63 35.65146714594921 39.0 35.5 41.0 28.0 41.0 64-65 35.396612180427894 39.0 35.0 40.5 28.0 41.0 66-67 35.040423389292265 38.5 35.0 40.0 26.5 41.0 68-69 34.63268562432393 37.5 34.5 40.0 26.0 41.0 70-71 34.197477958845454 37.0 34.0 39.5 26.0 41.0 72-73 33.765196495816824 37.0 34.0 39.0 26.0 41.0 74-75 33.31972404609326 36.0 34.0 39.0 25.5 40.0 76-77 32.91153721301903 36.0 33.5 37.5 24.5 40.0 78-79 32.45375324485413 35.0 33.0 37.0 23.0 39.0 80-81 31.958880192155515 35.0 32.0 37.0 21.0 39.0 82-83 31.519697288686494 35.0 32.0 36.0 19.0 38.5 84-85 31.106238831634723 35.0 32.0 36.0 18.0 37.0 86-87 30.853128801651906 35.0 32.0 35.5 17.5 37.0 88-89 30.44569957654848 35.0 31.0 35.0 9.0 36.5 90-91 30.0469421365893 34.0 31.0 35.0 4.5 36.0 92-93 29.70372394653082 34.0 31.0 35.0 2.0 36.0 94-95 29.30243025702667 34.0 30.0 35.0 2.0 35.5 96-97 28.895825014856584 34.0 30.0 35.0 2.0 35.0 98-99 28.481521832695684 34.0 30.0 35.0 2.0 35.0 100-101 25.091019778701728 30.0 24.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2841093148518503 1101 2 0.2741130810660657 1101 3 0.2966783502470882 1101 4 0.12799941394521852 1101 5 0.3218479844389819 1101 6 0.2693845359477862 1101 7 0.5212622227134744 1101 8 0.3242739197239999 1101 9 0.20957033411658443 1101 10-11 0.43389243137075795 1101 12-13 0.33187712271865166 1101 14-15 0.4607796277070477 1101 16-17 0.565572937496448 1101 18-19 0.6526620895634849 1101 20-21 0.5974701854886462 1101 22-23 0.5473040584237694 1101 24-25 0.6059353757996888 1101 26-27 0.6644285780077013 1101 28-29 0.6380328766696479 1101 30-31 0.8223724438688933 1101 32-33 0.6314222815599848 1101 34-35 0.5218480571368502 1101 36-37 0.5520680602693417 1101 38-39 0.8952313453523857 1101 40-41 0.717144740253147 1101 42-43 0.6538420104840696 1101 44-45 0.851730632571936 1101 46-47 0.8019048275533933 1101 48-49 0.8401311040126558 1101 50-51 1.0086965343236116 1101 52-53 0.8505584330144984 1101 54-55 0.9485559908119754 1101 56-57 0.8427943936251197 1101 58-59 0.8041642928451793 1101 60-61 0.56376244848299 1101 62-63 0.5026356009939548 1101 64-65 0.7171814735766091 1101 66-67 0.9366508128671001 1101 68-69 0.7884306949779969 1101 70-71 0.9134689722506195 1101 72-73 0.9451394500031469 1101 74-75 0.8905034427079173 1101 76-77 0.6455750611310336 1101 78-79 0.8635520841826434 1101 80-81 0.8021487013153248 1101 82-83 0.8974565594109158 1101 84-85 0.774976308836731 1101 86-87 0.7887850612939609 1101 88-89 0.6096922815321903 1101 90-91 0.6004538070295702 1101 92-93 0.6367054624688819 1101 94-95 0.848871527999993 1101 96-97 0.8130220580635594 1101 98-99 0.9704308897595588 1101 100-101 1.3534276957093176 1102 1 0.5859935056682808 1102 2 0.43859157935423454 1102 3 0.3438700833109145 1102 4 0.4037242985826026 1102 5 0.38048588291987784 1102 6 0.22993169555950743 1102 7 0.19412752461489902 1102 8 0.3533069106908542 1102 9 0.4705726766240872 1102 10-11 0.4463288742065217 1102 12-13 0.5636239752190306 1102 14-15 0.718304247539244 1102 16-17 0.6072570358660911 1102 18-19 0.5176913513210692 1102 20-21 0.5591815478252045 1102 22-23 0.6630215786562914 1102 24-25 0.8282785100590573 1102 26-27 0.725090904376188 1102 28-29 0.6423293017995064 1102 30-31 0.7021336491242565 1102 32-33 0.5690214855775011 1102 34-35 0.5566025639175791 1102 36-37 0.544423964720103 1102 38-39 0.6963070185294953 1102 40-41 0.602027340671512 1102 42-43 0.7539234890058566 1102 44-45 0.7082704591166973 1102 46-47 0.881766314066347 1102 48-49 0.9562050927780206 1102 50-51 0.6637948177905244 1102 52-53 0.6781436133248988 1102 54-55 0.8262982523110267 1102 56-57 0.9200086116271677 1102 58-59 0.9998255615597813 1102 60-61 0.9055969687390188 1102 62-63 0.9904788488531793 1102 64-65 0.7836992852515365 1102 66-67 1.0083691478848422 1102 68-69 0.9816985321149687 1102 70-71 1.0015793756476512 1102 72-73 0.8409981474436279 1102 74-75 0.8721770428834077 1102 76-77 0.8727395302279533 1102 78-79 0.9961270405054563 1102 80-81 0.9056178060700688 1102 82-83 1.027487662269717 1102 84-85 1.0991503594944412 1102 86-87 1.05555592645484 1102 88-89 1.070493278077187 1102 90-91 1.2666269018815406 1102 92-93 1.354108177897313 1102 94-95 1.3538041123576825 1102 96-97 1.4526660595991174 1102 98-99 1.6132047633581692 1102 100-101 1.5029227639084048 1103 1 0.41009291891121435 1103 2 0.3774440779270698 1103 3 0.3781338709248736 1103 4 0.43940495443222005 1103 5 0.46599969260342533 1103 6 0.3379579672097819 1103 7 0.5291661022734075 1103 8 0.3981287943163849 1103 9 0.46755649813694333 1103 10-11 0.4957794883298945 1103 12-13 0.3967660386970806 1103 14-15 0.5124390309923328 1103 16-17 0.6663653063746366 1103 18-19 0.48061442552788947 1103 20-21 0.5947547982945451 1103 22-23 0.4870466909169835 1103 24-25 0.5947218323870231 1103 26-27 0.6587143793669128 1103 28-29 0.5273930388005041 1103 30-31 0.6506165849105443 1103 32-33 0.5974243234336285 1103 34-35 0.4964518726977545 1103 36-37 0.5066371335025153 1103 38-39 0.5148272372245017 1103 40-41 0.460155132475748 1103 42-43 0.37693575381750577 1103 44-45 0.6801957226277082 1103 46-47 0.6962698658004243 1103 48-49 0.555774294211659 1103 50-51 0.4937161911669321 1103 52-53 0.6177817896109659 1103 54-55 0.6235956092558865 1103 56-57 0.6401300532722161 1103 58-59 0.6173351397656077 1103 60-61 0.5014753976190036 1103 62-63 0.4949221947220508 1103 64-65 0.5487787063806806 1103 66-67 0.5695394545639871 1103 68-69 0.6903430467897849 1103 70-71 0.6826001092999618 1103 72-73 0.7277987618612585 1103 74-75 0.766448952109684 1103 76-77 0.5824522134791863 1103 78-79 0.6533229067224937 1103 80-81 0.9350365811190642 1103 82-83 0.996225340077121 1103 84-85 0.9850211081540827 1103 86-87 0.7010210653167555 1103 88-89 0.8646346824098927 1103 90-91 1.062199151862412 1103 92-93 1.0527029329836175 1103 94-95 0.8751240803420508 1103 96-97 1.1232390452332766 1103 98-99 1.446115831703299 1103 100-101 1.3607334891448453 1104 1 0.1535203116783883 1104 2 0.14800443320008938 1104 3 0.19014462379483987 1104 4 0.2569964355442522 1104 5 0.21899787228004897 1104 6 0.2796146305514 1104 7 0.33190194122832395 1104 8 0.24412267397215714 1104 9 0.4177343037173884 1104 10-11 0.41669122800673364 1104 12-13 0.402474978570055 1104 14-15 0.385881471014649 1104 16-17 0.5633395118851965 1104 18-19 0.3456287119667607 1104 20-21 0.5027270256828018 1104 22-23 0.6934850281671103 1104 24-25 0.6191003582068646 1104 26-27 0.4280320680634375 1104 28-29 0.5326800074266984 1104 30-31 0.37763641121901514 1104 32-33 0.5018228277252064 1104 34-35 0.531622013758728 1104 36-37 0.4191496024358443 1104 38-39 0.515438708079607 1104 40-41 0.5046347059677743 1104 42-43 0.6116680028467556 1104 44-45 0.6168105692527845 1104 46-47 0.5764863884755727 1104 48-49 0.3993420153133229 1104 50-51 0.12640113996681634 1104 52-53 0.19562456551958007 1104 54-55 0.4100520933830083 1104 56-57 0.3709624144514194 1104 58-59 0.25219757291240086 1104 60-61 0.48149481252310977 1104 62-63 0.4185644194615392 1104 64-65 0.42208870801502485 1104 66-67 0.620802268682489 1104 68-69 0.446099889909469 1104 70-71 0.34197984679016713 1104 72-73 0.5347120457133556 1104 74-75 0.34180921270333897 1104 76-77 0.6025758038557072 1104 78-79 0.5680001797315555 1104 80-81 0.582802599119411 1104 82-83 0.7581236612092148 1104 84-85 0.5501497988174542 1104 86-87 0.4532062987182819 1104 88-89 0.46289762614893704 1104 90-91 0.7489612305251789 1104 92-93 0.4993126511434589 1104 94-95 0.39332097395739396 1104 96-97 0.595958146230231 1104 98-99 0.8335812427413352 1104 100-101 0.7971651762971952 1105 1 0.018129888535526106 1105 2 0.02749148885446928 1105 3 -0.014657488297373789 1105 4 -0.12931091787434923 1105 5 0.06617571248332155 1105 6 -0.08515458742177628 1105 7 0.15470969307895643 1105 8 0.02546800587811049 1105 9 -0.1611941421830494 1105 10-11 0.0659013398628403 1105 12-13 0.08855413909925858 1105 14-15 0.008033084463143325 1105 16-17 0.016707536977030202 1105 18-19 0.11174106174983223 1105 20-21 0.16641886735101252 1105 22-23 -0.01705544420961047 1105 24-25 0.14753324588659922 1105 26-27 0.0513313535688269 1105 28-29 -0.003488096010009656 1105 30-31 0.26460580573647974 1105 32-33 0.219383484641682 1105 34-35 0.038820620722304966 1105 36-37 0.05086962143180074 1105 38-39 0.150520581166262 1105 40-41 0.17844430643570064 1105 42-43 0.19177564657071855 1105 44-45 0.23686404231904845 1105 46-47 0.2786477145432187 1105 48-49 0.5022380929713464 1105 50-51 0.2949935614408261 1105 52-53 0.5073225030636834 1105 54-55 0.6791821968680125 1105 56-57 0.7061034486575508 1105 58-59 0.5990614969715224 1105 60-61 0.6389847980615002 1105 62-63 0.5401941808584425 1105 64-65 0.504577553259729 1105 66-67 0.4839804415832063 1105 68-69 0.6158097991957519 1105 70-71 0.7257631265967746 1105 72-73 0.7897326917366811 1105 74-75 0.7657214396029985 1105 76-77 0.7651722928243743 1105 78-79 0.6880663972566623 1105 80-81 0.7737031896573896 1105 82-83 0.7509800566469629 1105 84-85 0.7985272279783793 1105 86-87 0.7702273954929986 1105 88-89 0.6989048810998213 1105 90-91 0.7820223690480574 1105 92-93 0.7279884884544039 1105 94-95 0.8943868131733019 1105 96-97 0.7690439955880422 1105 98-99 0.770300309256676 1105 100-101 0.6861917947763949 1106 1 0.3869597053755278 1106 2 0.33557804921893464 1106 3 0.3068723151431918 1106 4 0.306137293300786 1106 5 0.3179124856926876 1106 6 0.39533634734358003 1106 7 0.3035387884107976 1106 8 0.3516566905687384 1106 9 0.4943269413160536 1106 10-11 0.44778916595875984 1106 12-13 0.4398525961025257 1106 14-15 0.39940616602448387 1106 16-17 0.4164952074333286 1106 18-19 0.5897827339995985 1106 20-21 0.562008906415528 1106 22-23 0.4718861548586162 1106 24-25 0.6251755118345059 1106 26-27 0.4423668723847385 1106 28-29 0.4512926685824894 1106 30-31 0.575173838802165 1106 32-33 0.6644316195430946 1106 34-35 0.47659054207791485 1106 36-37 0.39031344171441873 1106 38-39 0.4167519379108171 1106 40-41 0.30223062664101974 1106 42-43 0.34306946530725213 1106 44-45 0.313282063531382 1106 46-47 0.3435100095575976 1106 48-49 0.45832143747087173 1106 50-51 0.5097427342832574 1106 52-53 0.5697265167104959 1106 54-55 0.5927197513676461 1106 56-57 0.4890145279306779 1106 58-59 0.40961793437038096 1106 60-61 0.5959107610172154 1106 62-63 0.37751599411151204 1106 64-65 0.39485677691696 1106 66-67 0.6730516544384173 1106 68-69 0.7603137108006948 1106 70-71 0.7304632368844537 1106 72-73 0.7806468425731126 1106 74-75 0.9210853373962209 1106 76-77 0.8225824392905281 1106 78-79 0.9408210123320515 1106 80-81 1.1260182001192618 1106 82-83 0.9310826654143192 1106 84-85 1.105507046364476 1106 86-87 1.0298195887747248 1106 88-89 0.8204223158291057 1106 90-91 0.8949461262502538 1106 92-93 0.9680561556123841 1106 94-95 1.0867112547775228 1106 96-97 1.2697539080794122 1106 98-99 1.1295469516669279 1106 100-101 1.0440753021306222 1107 1 0.011305371243683027 1107 2 0.03598174010705435 1107 3 0.14978776186486797 1107 4 0.06421111106318023 1107 5 -0.07182666788729364 1107 6 -0.01831372807119891 1107 7 -0.058518704269530986 1107 8 -0.04294006215950219 1107 9 0.042902932798618565 1107 10-11 0.030823058248707014 1107 12-13 0.14929497033728012 1107 14-15 0.17898428686558532 1107 16-17 0.10705061511676917 1107 18-19 0.07291422188887253 1107 20-21 0.15849546483980248 1107 22-23 0.20430785657097061 1107 24-25 0.28025270108124545 1107 26-27 0.1555356240482979 1107 28-29 0.1617775412645983 1107 30-31 0.13783464973287352 1107 32-33 0.22752495642536275 1107 34-35 0.035768964578132056 1107 36-37 0.2762153137754666 1107 38-39 0.200533323768326 1107 40-41 0.1781178593621604 1107 42-43 -0.2866328681148076 1107 44-45 0.10564742596595522 1107 46-47 0.27389551217493846 1107 48-49 0.2965567315885238 1107 50-51 0.07743859090645344 1107 52-53 0.13403979858433246 1107 54-55 0.35762526108283765 1107 56-57 0.23637501058527732 1107 58-59 0.39676415032283074 1107 60-61 0.38069028368600755 1107 62-63 0.497880203143545 1107 64-65 0.36211788950678425 1107 66-67 0.32374813212451414 1107 68-69 0.41239648961357744 1107 70-71 0.42314800309862477 1107 72-73 0.2682699461131719 1107 74-75 0.5040415968230647 1107 76-77 0.5174298352624902 1107 78-79 0.450314402448285 1107 80-81 0.5571689409585829 1107 82-83 0.637020470400568 1107 84-85 0.7434384632751296 1107 86-87 0.47125113877028824 1107 88-89 0.5119314986877654 1107 90-91 0.5293175218673234 1107 92-93 0.4315798003643998 1107 94-95 0.6967392026082386 1107 96-97 0.7009157393277476 1107 98-99 0.515337919637826 1107 100-101 0.6854114746086601 1108 1 0.23117363671487468 1108 2 0.18201410791914086 1108 3 0.08800818506929531 1108 4 0.1189687151777079 1108 5 0.17111690243761757 1108 6 0.1096921912776736 1108 7 0.08429284136240511 1108 8 0.03361975155510777 1108 9 0.0027696023669321335 1108 10-11 0.03668256076225873 1108 12-13 -0.0229700510985964 1108 14-15 -0.007339188140399244 1108 16-17 0.13731110999174234 1108 18-19 0.2035914650391888 1108 20-21 0.165950870235406 1108 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2316 96-97 -3.2798295277681078 2316 98-99 -3.0902924843802673 2316 100-101 -3.2101456079100643 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6826.0 3 1253.0 4 907.0 5 756.0 6 710.0 7 831.0 8 1018.0 9 1238.0 10 1570.0 11 2056.0 12 3001.0 13 4618.0 14 4573.0 15 4378.0 16 5298.0 17 7423.0 18 6487.0 19 3935.0 20 3663.0 21 3801.0 22 4130.0 23 4514.0 24 5107.0 25 6078.0 26 7256.0 27 8612.0 28 10354.0 29 12582.0 30 15565.0 31 19433.0 32 24874.0 33 32092.0 34 44246.0 35 67029.0 36 102202.0 37 198607.0 38 280128.0 39 57059.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.682218514460274 23.84429890095295 14.768029684231498 21.705452900355283 2 13.220888549592983 24.640385524972825 40.83558762270664 21.30313830272755 3 17.04475323848237 30.547168421827823 31.65969822914214 20.748380110547664 4 9.659087962545208 19.143721561708823 45.34485852290466 25.85233195284131 5 9.034456291747915 42.564514790689955 38.06351906401693 10.337509853545201 6 24.51945266402603 44.651878680012985 18.67966034718268 12.149008308778306 7 24.39790369570606 34.99029738439056 26.062853865437013 14.548945054466367 8 22.522190806710242 36.19627065834974 23.040892486345317 18.2406460485947 9 24.787515429377535 18.5033244473949 25.20543114089226 31.503728982335303 10-11 21.295529007159356 29.024064684145184 31.478137675755786 18.20226863293967 12-13 23.260258187439746 27.869422062771708 30.3310862635217 18.53923348626684 14-15 20.735722646232578 30.03275974819023 28.778637553344144 20.452880052233052 16-17 17.595702614446886 32.96678104785338 30.115048525561498 19.322467812138235 18-19 17.291323947051247 33.59006619631533 31.647844315228056 17.47076554140537 20-21 18.46179455573262 31.650346379364613 31.058449670370475 18.82940939453229 22-23 18.649299569971436 30.90692879116958 31.17478908270945 19.268982556149535 24-25 19.79426433915212 31.49006643125962 30.749820540163736 17.965848689424526 26-27 18.23202299662446 30.95697052766632 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18.14323405577209 32.2108273037443 30.87458837044211 18.7713502700415 54-55 18.521637665834746 30.829250599186075 30.951042106925335 19.698069628053844 56-57 19.450899057038857 30.91015683014974 29.987206578580526 19.65173753423087 58-59 19.6617433101131 29.9971964798626 30.94367113145487 19.397389078569432 60-61 19.18213381815226 32.324613992111786 30.759947411945493 17.733304777790458 62-63 18.11598658526672 32.05456353135141 31.91882168784691 17.910628195534954 64-65 18.038720477303784 32.10251524668305 31.027925064806873 18.8308392112063 66-67 18.89308890457744 31.60091804064492 29.453578248376367 20.052414806401273 68-69 17.854648702399242 31.148776404908972 31.04735737231938 19.949217520372407 70-71 20.010233787861498 30.134284596221317 31.4698877044999 18.385593911417292 72-73 19.17089373428203 29.91778974477092 31.696232690040866 19.21508383090618 74-75 18.055834243230496 32.68218573368114 29.981263501874956 19.280716521213417 76-77 18.034445023449713 30.665659197498695 31.65841584158416 19.64147993746743 78-79 17.6631028109072 30.58563649921912 31.817430949317583 19.933829740556096 80-81 19.393411953536607 31.291188318489993 29.796449062758935 19.51895066521447 82-83 19.7376361299844 30.01123538123691 31.154471900603355 19.096656588175335 84-85 17.751391407666812 30.6703249744648 31.307610530923647 20.27067308694474 86-87 18.24265309781 31.4533712994501 31.142568883070755 19.161406719669145 88-89 18.457582127312254 30.862899026757017 30.506291706039235 20.173227139891495 90-91 18.4296752445454 29.332257992201864 31.705376688279497 20.532690074973242 92-93 20.227233513294294 31.07144104475706 30.634978802415997 18.066346639532647 94-95 19.058771171447226 29.116110806251648 31.852862838778336 19.972255183522787 96-97 18.298143027130433 29.489994102532574 32.35485040646679 19.8570124638702 98-99 18.423381771865714 30.87671225740578 30.800551152184163 19.899354818544342 100-101 18.018290713352407 29.63655100122744 31.505269234215742 20.83988905120441 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 985.0 1 620.0 2 445.5 3 627.5 4 426.5 5 449.0 6 1063.0 7 1718.0 8 2030.5 9 1871.5 10 1408.5 11 1238.5 12 1367.5 13 1416.0 14 1381.0 15 1291.5 16 1233.5 17 1211.0 18 1123.0 19 1070.5 20 1189.0 21 1441.5 22 1743.5 23 2171.5 24 2431.0 25 3105.5 26 5814.0 27 8938.5 28 9099.0 29 10308.5 30 16026.5 31 21448.5 32 30018.5 33 40898.0 34 48664.0 35 56818.0 36 69776.0 37 78153.0 38 72077.0 39 63890.5 40 54771.5 41 44203.0 42 34017.0 43 32759.5 44 35470.0 45 31890.0 46 25592.5 47 26759.5 48 41187.0 49 37183.0 50 18389.5 51 7684.5 52 3000.0 53 1501.5 54 705.5 55 464.0 56 345.0 57 292.5 58 261.0 59 223.0 60 214.0 61 186.0 62 126.0 63 89.5 64 60.0 65 50.5 66 38.0 67 29.0 68 29.0 69 20.0 70 13.5 71 10.0 72 10.0 73 9.5 74 6.0 75 9.0 76 7.5 77 5.0 78 4.5 79 6.5 80 8.0 81 4.5 82 2.5 83 5.5 84 6.0 85 6.5 86 6.0 87 2.0 88 3.5 89 4.0 90 2.0 91 3.0 92 6.5 93 7.5 94 6.5 95 4.5 96 4.0 97 4.0 98 4.5 99 5.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.049262667506033385 2 0.013586125143769208 3 0.011408196685607731 4 0.0072597615272049195 5 0.010267377017046957 6 0.006015230979684076 7 0.004770700432163233 8 0.008400581195765693 9 0.01545292096505047 10-11 0.002696482852961827 12-13 0.007726460482525235 14-15 0.008141303998365516 16-17 0.019808777881373425 18-19 0.012134172838328223 20-21 0.003163181808282143 22-23 0.010059955259126818 24-25 0.02229783897641511 26-27 0.008037593119405446 28-29 0.018512391894372544 30-31 0.01493436657025012 32-33 0.01493436657025012 34-35 0.2695445744172226 36-37 0.47504768107660184 38-39 0.4712622339945593 40-41 0.4905524574811324 42-43 0.481114767495766 44-45 0.4954268687922557 46-47 0.5035681727906212 48-49 0.5111390669547063 50-51 0.522547263640314 52-53 0.5104649462414659 54-55 0.49703438741613676 56-57 0.4917451325891732 58-59 0.48858195078089106 60-61 0.48593732336740925 62-63 0.4850557808962487 64-65 0.5009235453771395 66-67 0.47359572877116085 68-69 0.5012346780140197 70-71 0.4825148643617269 72-73 0.49034503572321225 74-75 0.5041904380643817 76-77 0.48941163781257163 78-79 0.489982047646852 80-81 0.49304151857617406 82-83 0.49262667506033375 84-85 0.49356007297097443 86-87 0.494389760002655 88-89 0.5082351623438244 90-91 0.5334369059311215 92-93 0.5216138657296735 94-95 0.4937156392894145 96-97 0.46514329213591515 98-99 0.45752054253235 100-101 0.46638782268343604 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 964219.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.582584827264148 #Duplication Level Percentage of deduplicated Percentage of total 1 74.32668309707704 8.60895111901075 2 8.845755330345966 2.0491342294991224 3 3.407515803342978 1.1840352252738955 4 1.8771881492976084 0.8697076390389817 5 1.2059977346469974 0.6984285531518624 6 0.8925961292854896 0.6203142230362092 7 0.7341267592242735 0.5952159823875769 8 0.5738717765083953 0.5317534825105009 9 0.4888205202295181 0.5095624626779205 >10 4.883728733577214 12.592538792820635 >50 1.1414764681704797 9.297700088618784 >100 1.3939442046599504 34.44254359919521 >500 0.15935906771321207 12.583932018271128 >1k 0.06625039893695332 12.917625948712615 >5k 0.0017905513226203604 1.1721802951244853 >10k+ 8.952756613101802E-4 1.3263763406703197 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12791 1.326565852778259 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6101 0.6327400725353888 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5203 0.5396077032292456 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4257 0.44149721173301915 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3954 0.4100728154081179 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3446 0.35738768889640216 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3153 0.3270004013611016 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2952 0.30615451469012744 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2711 0.2811601928607505 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2672 0.27711546858130776 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 2557 0.2651887175008997 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2554 0.26487758486401947 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2443 0.2533656772994517 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2424 0.25139517059921035 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2341 0.2427871676455245 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2250 0.2333494776601581 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 2186 0.22671198140671364 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 2108 0.21862253284782815 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 2103 0.2181039784530278 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 2068 0.21447409768942535 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1983 0.20565867297781937 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1972 0.20451785330925856 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1962 0.2034807445196579 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1905 0.19756922441893388 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1900 0.19705067002413354 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1868 0.19373192189741129 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1806 0.18730184740188693 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1798 0.18647216037020636 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1726 0.1790049770850813 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1715 0.17786415741652054 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1710 0.17734560302172017 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1692 0.1754788072004389 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1675 0.1737157222581177 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1674 0.17361201137915766 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1621 0.16811533479427393 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1614 0.16738935864155344 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1584 0.1642780322727513 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1520 0.15764053601930683 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1516 0.15722569250346655 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1503 0.15587745107698564 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1455 0.15089932888690227 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1446 0.14996593097626162 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1414 0.14664718284953937 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1389 0.1440544108755376 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1389 0.1440544108755376 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1384 0.14353585648073727 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1383 0.1434321456017772 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1318 0.13669093846937264 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1300 0.13482414264809137 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 1234 0.12797922463672673 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1218 0.1263198505733656 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1212 0.12569758529960517 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 1189 0.12331223508352356 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 1182 0.12258625893080306 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1173 0.12165286102016244 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1156 0.11988977607784125 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1156 0.11988977607784125 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1142 0.11843782377240027 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1130 0.11719329322487941 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1128 0.11698587146695928 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1127 0.1168821605879992 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 1121 0.11625989531423879 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 1116 0.11574134091943843 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 1113 0.11543020828255822 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 1099 0.11397825597711723 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 1089 0.11294114718751654 No Hit ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT 1081 0.11211146015583597 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 1077 0.11169661663999568 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 1077 0.11169661663999568 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 1069 0.11086692960831514 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1067 0.11065950785039498 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1058 0.10972610993975436 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1057 0.10962239906079428 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 1045 0.10837786851327343 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 1031 0.10692591620783244 No Hit CTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCT 1023 0.1060962291761519 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1001 0.10381458983903034 No Hit GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG 997 0.10339974632319006 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 989 0.10257005929150952 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 989 0.10257005929150952 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 987 0.10236263753358936 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 982 0.10184408313878902 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 978 0.10142923962294874 No Hit GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA 969 0.10049584171230809 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.185543948003514E-5 0.0 12-13 0.0 0.0 0.0 2.0742175792014055E-4 0.0 14-15 0.0 0.0 0.0 5.704098342803865E-4 0.0 16-17 0.0 0.0 0.0 0.0019186512607613 0.0 18-19 0.0 0.0 0.0 0.003059470929322073 0.0 20-21 0.0 0.0 0.0 0.005963375540204041 0.0 22-23 0.0 0.0 0.0 0.01322313706740896 0.0 24-25 0.0 0.0 0.0 0.019601356123453283 0.0 26-27 0.0 0.0 0.0 0.02333494776601581 0.0 28-29 0.0 0.0 0.0 0.028209359077139118 0.0 30-31 0.0 0.0 0.0 0.04127692982610797 0.0 32-33 0.0 0.0 0.0 0.06710093868716546 0.0 34-35 0.0 0.0 0.0 0.10282933648890968 0.0 36-37 0.0 0.0 0.0 0.15634415003230595 0.0 38-39 0.0 0.0 0.0 0.23184566991523708 0.0 40-41 0.0 0.0 0.0 0.3146069513253732 0.0 42-43 0.0 0.0 0.0 0.39560514779318806 0.0 44-45 0.0 0.0 0.0 0.48858195078089106 0.0 46-47 0.0 0.0 0.0 0.6077457507060118 0.0 48-49 0.0 0.0 0.0 0.7284652138155336 0.0 50-51 0.0 0.0 0.0 0.8546813535099391 0.0 52-53 0.0 0.0 0.0 0.9778898777145026 0.0 54-55 0.0 0.0 0.0 1.10700992201979 0.0 56-57 0.0 0.0 0.0 1.2774587515906657 0.0 58-59 0.0 0.0 0.0 1.4414256512265369 0.0 60-61 0.0 0.0 0.0 1.620482483751098 0.0 62-63 0.0 0.0 0.0 1.7836196963552886 0.0 64-65 0.0 0.0 0.0 1.9667731085987725 0.0 66-67 0.0 0.0 0.0 2.1922405594579653 0.0 68-69 0.0 0.0 0.0 2.427197555742005 0.0 70-71 0.0 0.0 0.0 2.6982977933436283 0.0 72-73 0.0 0.0 0.0 2.9433147448867945 0.0 74-75 0.0 0.0 0.0 3.208347896069254 0.0 76-77 0.0 0.0 0.0 3.4947454883174878 0.0 78-79 0.0 0.0 0.0 3.86644527851038 0.0 80-81 0.0 0.0 0.0 4.260391052240207 0.0 82-83 0.0 0.0 0.0 4.6048667367060805 0.0 84-85 0.0 0.0 0.0 4.968684500098007 0.0 86-87 0.0 0.0 0.0 5.3540222708741485 0.0 88-89 0.0 0.0 0.0 5.794067530301726 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGCTCG 15 6.72107E-4 94.629906 9 CATGTAT 20 0.0021028689 71.00189 1 GGAGATA 20 0.0021063336 70.972435 8 ATGTATT 65 6.91216E-10 58.257965 1 CATGAGA 25 0.0050971084 56.78384 4 TGGGTAT 335 0.0 56.49254 6 GAGGCCG 35 2.8419765E-4 54.074234 7 TACCTGG 200 0.0 49.688435 2 CATGGGT 450 0.0 49.422966 4 GGCCGCT 40 5.492425E-4 47.314953 9 AGGCCGC 40 5.492425E-4 47.314953 8 ACCTGGG 215 0.0 46.224197 3 GTATGGT 115 1.8189894E-12 45.276573 1 ATGGGTA 505 0.0 42.166218 5 GCTTTGC 45 9.790937E-4 42.075195 1 CATATAG 45 9.800943E-4 42.066467 3 CCTTTAG 220 0.0 40.87988 1 CTTTAGG 220 0.0 40.869274 2 TAGGCAT 220 0.0 40.867153 5 CATTCCG 220 0.0 38.712234 9 >>END_MODULE