FastQCFastQC Report
Thu 26 May 2016
SRR936327_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936327_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964219
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT131171.3603755993192417No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT62410.6472595955897986No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT52900.5486305496987718No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43170.44771986447062334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39550.4101765262870779No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36080.37418885128793355No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32290.3348824281620669No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29390.3048062732636465No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28750.29816877701020206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28640.2970279573416413No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27780.2881088217510752No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA26730.27721917946026786No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25400.2634256325585785No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT24790.25709926894201424No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24390.2529508337836114No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT23440.24309830028240473No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT23360.24226861325072416No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA23200.24060923918736302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22240.23065299480719628No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG21830.2264008487698334No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA21160.21945221987950872No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT21150.21934850900054864No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG19820.2055549620988593No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA19430.20151023781941657No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA19200.19912488760333494No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG19000.19705067002413354No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT18950.1965321156293332No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC18800.1949764524449321No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA17460.1810791946642827No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA17060.1769307595058799No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG17000.17630849423211947No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC16590.1720563481947566No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG16580.17195263731579652No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG16220.168219045673234No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC16150.16749306952051352No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT15880.1646928757885916No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG15870.16458916490963155No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA15810.1639668996358711No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT15550.1612704167829093No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC15420.1599221753564284No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC15390.15961104271954815No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT14570.1511067506448224No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA14540.1507956180079422No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT14290.14820284603394043No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC14270.1479954242760203No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC13900.1441581217544977No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG13900.1441581217544977No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG13720.14229132593321642No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG13520.14021710835401502No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC12830.13306105770577017No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG12780.1325425033109698No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC12440.12901633342632743No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT12410.12870520078944722No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT12290.12746067024192637No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT12160.12611242881544546No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC12080.1252827417837649No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC12040.12486789826792462No Hit
GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT11890.12331223508352356No Hit
CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA11880.12320852420456348No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA11860.12300110244664335No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT11760.12196399365704265No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG11710.12144543926224229No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT11560.11988977607784125No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC11500.11926751080408081No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT11340.1176081367407197No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC11300.11719329322487941No Hit
ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT11220.11636360619319884No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT11170.1158450517983985No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA11150.11563763004047835No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT11080.11491165388775786No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG10980.11387454509815716No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG10950.11356341246127695No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA10720.11117806224519534No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10550.10941497730287415No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA10370.10754818148159288No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC10360.1074444706026328No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10260.1064073618130321No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC10180.10557767478135154No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG10130.1050591203865512No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA9910.10277748104942964No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT9850.10215521577566923No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA9810.10174037225982895No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT9770.10132552874398866No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC9740.10101439610710845No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG9660.10018470907542788No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGATG200.00208564671.149275
TGGATGA250.005049225456.9194156
ATGTATT558.1112194E-751.890231
GGGCATC555.0043185E-543.120777
GATTGGC459.691545E-442.162539
CATTCCG2150.041.91749
TAGGCAT2400.041.503745
CTCCCGC1850.041.0237
ACTCCCG1850.041.0236
CCTTTAG2350.040.481741
TACTCCC1900.039.943455
GGCATTC2400.039.527377
GTATGGT853.600435E-739.1720351
CTTTAGG2500.037.9541472
GGGACCA500.001626954937.9462787
GGCATCT651.3391257E-436.4868058
TGGGTAT3150.036.1393136
GATGACC4650.035.702149
GTGGTAC2000.035.6745341
GGTACTC2150.035.2988623
Produced by FastQC (version 0.11.5)