##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936327_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 964219 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4613651048154 34.0 31.0 34.0 31.0 34.0 2 32.60465724073058 34.0 31.0 34.0 31.0 34.0 3 32.697345727474776 34.0 31.0 34.0 31.0 34.0 4 36.17452466711401 37.0 35.0 37.0 35.0 37.0 5 36.158069899058205 37.0 35.0 37.0 35.0 37.0 6 36.20748916999146 37.0 37.0 37.0 35.0 37.0 7 36.16181801022382 37.0 36.0 37.0 35.0 37.0 8 36.14234940402543 37.0 36.0 37.0 35.0 37.0 9 37.90808104797769 39.0 38.0 39.0 35.0 39.0 10-11 37.8391874667477 39.0 38.0 39.0 35.0 39.0 12-13 37.8393570340348 39.0 38.0 39.0 35.0 39.0 14-15 39.12103889261672 40.5 38.0 41.0 36.0 41.0 16-17 39.08429360964677 40.0 38.0 41.0 36.0 41.0 18-19 39.19301320550622 40.5 39.0 41.0 36.0 41.0 20-21 39.14884377926592 40.0 39.0 41.0 36.0 41.0 22-23 39.02371193681103 40.0 38.5 41.0 35.0 41.0 24-25 39.17584749937514 40.5 39.0 41.0 36.0 41.0 26-27 39.07565812331016 41.0 39.0 41.0 35.5 41.0 28-29 39.02992525556954 40.0 39.0 41.0 35.0 41.0 30-31 38.95587309522007 40.0 38.0 41.0 35.0 41.0 32-33 38.791333192977945 40.0 38.0 41.0 35.0 41.0 34-35 38.66027997788884 40.0 38.0 41.0 35.0 41.0 36-37 38.52835507286208 40.0 38.0 41.0 34.5 41.0 38-39 38.41677927939607 40.0 38.0 41.0 34.0 41.0 40-41 38.40594149254474 40.0 38.0 41.0 34.0 41.0 42-43 38.40897348009114 40.0 38.0 41.0 35.0 41.0 44-45 38.28294142720689 40.0 38.0 41.0 34.0 41.0 46-47 38.099941507064266 40.0 38.0 41.0 34.0 41.0 48-49 37.903135594714485 40.0 38.0 41.0 33.0 41.0 50-51 37.745866862196245 40.0 38.0 41.0 33.0 41.0 52-53 37.691146928239334 40.0 38.0 41.0 33.0 41.0 54-55 37.60328462724755 40.0 38.0 41.0 33.0 41.0 56-57 37.407892294177984 40.0 38.0 41.0 32.5 41.0 58-59 37.245772485296385 40.0 37.0 41.0 32.0 41.0 60-61 37.01932289241344 40.0 37.0 41.0 31.0 41.0 62-63 36.81624091622339 39.0 36.5 41.0 31.0 41.0 64-65 36.524041218851735 39.0 36.0 41.0 31.0 41.0 66-67 36.18863245797894 39.0 36.0 40.0 31.0 41.0 68-69 35.80129514145646 38.0 35.0 40.0 30.0 41.0 70-71 35.41692032619146 37.0 35.0 39.5 29.5 41.0 72-73 34.98011706884017 37.0 34.5 39.0 29.0 41.0 74-75 34.53295828022472 36.5 34.0 39.0 29.0 40.0 76-77 33.744965614658085 36.0 34.0 37.5 28.5 39.0 78-79 33.74103549089989 35.5 34.0 37.0 29.0 39.0 80-81 33.48975336515875 35.0 34.0 37.0 29.0 39.0 82-83 33.131672369036494 35.0 34.0 36.5 28.5 39.0 84-85 32.75490007975367 35.0 33.5 36.0 27.5 37.0 86-87 32.432654303638486 35.0 33.0 36.0 27.0 37.0 88-89 31.8875556279227 35.0 33.0 35.0 25.5 36.5 90-91 31.644523702602832 35.0 33.0 35.0 25.0 36.0 92-93 31.38622035035609 35.0 32.0 35.0 25.0 36.0 94-95 31.24362670721071 35.0 32.0 35.0 24.5 36.0 96-97 31.027868150285357 34.5 32.0 35.0 24.5 35.0 98-99 30.73216561797683 34.0 32.0 35.0 21.0 35.0 100-101 27.462386656973155 31.5 26.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.032569090141201684 1101 2 -0.003998232515478151 1101 3 -0.08774160767436001 1101 4 -0.04847822020050785 1101 5 -0.05616611925761106 1101 6 0.13059157808613975 1101 7 0.09317813783349749 1101 8 0.05193934846920456 1101 9 0.14612619428578455 1101 10-11 0.12287863368003826 1101 12-13 0.1814109870146936 1101 14-15 0.2518871188368479 1101 16-17 0.17288061473328753 1101 18-19 0.134685514360811 1101 20-21 0.04917451550011975 1101 22-23 0.2187663867148828 1101 24-25 0.06999673489806213 1101 26-27 0.041099549161600635 1101 28-29 0.09637278204593969 1101 30-31 0.13310221873719286 1101 32-33 0.20565657020375738 1101 34-35 0.32408477446477946 1101 36-37 0.11150916615122242 1101 38-39 0.069095154886476 1101 40-41 0.06883808666865576 1101 42-43 0.09456409826341883 1101 44-45 0.20609534967207566 1101 46-47 0.26957796555947766 1101 48-49 0.3021345086828049 1101 50-51 0.3736771724274064 1101 52-53 0.39079556674087 1101 54-55 0.340655423776802 1101 56-57 0.3442498595208079 1101 58-59 0.30931612611146875 1101 60-61 0.3189688446650649 1101 62-63 0.3347751930963412 1101 64-65 0.2919738468574664 1101 66-67 0.2659279455024759 1101 68-69 0.3897770687096198 1101 70-71 0.2597064186214979 1101 72-73 0.3582747900138088 1101 74-75 0.38971723240826606 1101 76-77 0.1745243549628981 1101 78-79 0.3794743440955486 1101 80-81 0.3218207815059202 1101 82-83 0.17688590219982814 1101 84-85 0.46725379672839296 1101 86-87 0.29641234874821265 1101 88-89 0.29013673679366647 1101 90-91 0.46236979771518705 1101 92-93 0.6748478798665332 1101 94-95 0.4765641143703725 1101 96-97 0.41566613331939806 1101 98-99 0.4167746263491665 1101 100-101 0.9079133225167837 1102 1 0.16218808036044408 1102 2 0.2107540806127446 1102 3 0.3147638568707336 1102 4 0.1756535243016515 1102 5 0.16836048269630766 1102 6 0.20304635358930767 1102 7 0.2212435713540728 1102 8 0.22028494275316746 1102 9 0.22717852073261469 1102 10-11 0.1305438823070446 1102 12-13 0.23421612016156956 1102 14-15 0.3651759523621223 1102 16-17 0.4163259026893513 1102 18-19 0.4552290701148394 1102 20-21 0.2724538693127627 1102 22-23 0.4120663451921729 1102 24-25 0.45065420389313005 1102 26-27 0.4688429858316354 1102 28-29 0.40377322547713135 1102 30-31 0.3083844556359949 1102 32-33 0.4481021793732225 1102 34-35 0.5312760374602092 1102 36-37 0.5044181844133817 1102 38-39 0.4907927285501117 1102 40-41 0.5876611920971655 1102 42-43 0.6210978922637764 1102 44-45 0.6396096593374665 1102 46-47 0.7610955813725795 1102 48-49 0.7673094916428269 1102 50-51 0.6698970390846668 1102 52-53 0.645610242276156 1102 54-55 0.5163912766767353 1102 56-57 0.688972950063814 1102 58-59 0.653547211734427 1102 60-61 0.7449863468782993 1102 62-63 0.8858066014995956 1102 64-65 0.8770797297634303 1102 66-67 0.9203156422456829 1102 68-69 0.744030012512944 1102 70-71 0.8377550080245868 1102 72-73 0.7471725579723767 1102 74-75 0.7813641170299732 1102 76-77 0.5342567293567058 1102 78-79 0.5155724630385308 1102 80-81 0.6705660906150683 1102 82-83 0.7633087600589761 1102 84-85 0.8454285211507937 1102 86-87 0.7361315612269621 1102 88-89 0.9634462533283248 1102 90-91 1.0567799010518861 1102 92-93 0.9431715376254033 1102 94-95 0.8917488904219546 1102 96-97 0.9633506547506023 1102 98-99 0.9022213088414723 1102 100-101 1.0829472066135857 1103 1 -0.007525662231529395 1103 2 -0.045510742801035065 1103 3 0.03384434911106382 1103 4 -0.03757705298020397 1103 5 -0.06729280685093642 1103 6 -0.02656869375734061 1103 7 0.07959205914517042 1103 8 0.07804637921920943 1103 9 0.02670858541124943 1103 10-11 0.06178089802648401 1103 12-13 0.0571782134188652 1103 14-15 -0.062415901307609545 1103 16-17 0.105039362790464 1103 18-19 0.08620625309241348 1103 20-21 0.1810026019292721 1103 22-23 0.2184159158649166 1103 24-25 0.13624334447353448 1103 26-27 0.16402872357417664 1103 28-29 0.13722396907547108 1103 30-31 0.05192174148731965 1103 32-33 0.0990486708955558 1103 34-35 0.07636054931011671 1103 36-37 0.2721246234394954 1103 38-39 0.2440928692070301 1103 40-41 0.05275223654827954 1103 42-43 0.17855977071030793 1103 44-45 0.18096735162252742 1103 46-47 0.10360030426669198 1103 48-49 0.16229130629350408 1103 50-51 0.10860387830617668 1103 52-53 4.799718001891051E-5 1103 54-55 0.2120684308166929 1103 56-57 0.07260142749691312 1103 58-59 0.09412702423111341 1103 60-61 -0.08890608295509139 1103 62-63 0.009861287022523868 1103 64-65 0.10493288748160978 1103 66-67 0.06968636442180554 1103 68-69 0.04179303799008238 1103 70-71 -0.022712896893693824 1103 72-73 0.06364766052244875 1103 74-75 0.14398307216689687 1103 76-77 0.11850006580920791 1103 78-79 0.22036362578268864 1103 80-81 0.14867599133129517 1103 82-83 0.31764337637164886 1103 84-85 0.2546452269542243 1103 86-87 0.2369915399224496 1103 88-89 0.3922784184442314 1103 90-91 0.7312319864818342 1103 92-93 0.7357246665059733 1103 94-95 0.3430971957710369 1103 96-97 0.07055520167645213 1103 98-99 0.11698719453860562 1103 100-101 0.8289202405066014 1104 1 0.059371221803161234 1104 2 0.07988164114630081 1104 3 -1.1059929432377658E-4 1104 4 -9.423405969286591E-4 1104 5 0.02403614192640191 1104 6 0.03509969179053485 1104 7 0.0036400525627016123 1104 8 0.11198301167732438 1104 9 0.17815188205633348 1104 10-11 0.1129021157833705 1104 12-13 0.14420573242518486 1104 14-15 0.2671422335090341 1104 16-17 0.22062355697332237 1104 18-19 0.3012058867734595 1104 20-21 0.24788187447620658 1104 22-23 0.31424776983327973 1104 24-25 0.20210044627075519 1104 26-27 0.2770219156774729 1104 28-29 0.23303187142047932 1104 30-31 0.11544637784532341 1104 32-33 0.07899200417253383 1104 34-35 0.15550727977262824 1104 36-37 0.22920401884416464 1104 38-39 0.3765058966358552 1104 40-41 0.4383233559626163 1104 42-43 0.342528357451819 1104 44-45 0.31650324806133767 1104 46-47 0.4149862015508603 1104 48-49 0.48108389087091297 1104 50-51 0.48493882907854413 1104 52-53 0.6193214458192884 1104 54-55 0.5339874067016339 1104 56-57 0.7132819917408071 1104 58-59 0.7305272924892989 1104 60-61 0.6167369195077015 1104 62-63 0.5020840540267741 1104 64-65 0.40043040496629345 1104 66-67 0.3650824397369803 1104 68-69 0.5032083816817945 1104 70-71 0.5003687189673514 1104 72-73 0.4464932260907233 1104 74-75 0.5124738662900157 1104 76-77 0.4346452915462109 1104 78-79 0.3520242904543309 1104 80-81 0.4559245979103821 1104 82-83 0.46473522662046207 1104 84-85 0.6312781640995269 1104 86-87 0.5259037699031168 1104 88-89 0.7136450980729556 1104 90-91 0.7065371583417779 1104 92-93 0.8371992411533284 1104 94-95 0.7168002858122371 1104 96-97 0.7385300496540879 1104 98-99 0.8209796483683292 1104 100-101 0.6851034225220367 1105 1 -0.02124485875254578 1105 2 0.04518854113047155 1105 3 0.0717131461813949 1105 4 0.02605713084340522 1105 5 -0.05900406885353249 1105 6 -0.005544399744195516 1105 7 -0.06420473870989696 1105 8 -0.1831142620817019 1105 9 -0.10784853113243997 1105 10-11 0.003026770006080426 1105 12-13 0.02684066941389318 1105 14-15 0.02825204781556323 1105 16-17 -0.033649861305192985 1105 18-19 0.014667523269309868 1105 20-21 0.024684142184959512 1105 22-23 -0.2351785953665697 1105 24-25 -0.03211721835412362 1105 26-27 0.01137507402267346 1105 28-29 0.0403094940288824 1105 30-31 -0.03387977566235634 1105 32-33 -0.1223181261965749 1105 34-35 -0.017803274517419254 1105 36-37 0.14872678581374998 1105 38-39 0.030152421691461484 1105 40-41 0.13262738777279282 1105 42-43 0.1481840799821441 1105 44-45 0.05650958004510187 1105 46-47 0.14674016105300325 1105 48-49 0.0838793543801799 1105 50-51 0.11643213085928039 1105 52-53 0.20466974517186287 1105 54-55 0.14710319883012346 1105 56-57 0.3411652578638851 1105 58-59 0.31374860873700783 1105 60-61 0.15244823470902702 1105 62-63 0.3778899733583714 1105 64-65 0.21785127529209802 1105 66-67 0.2952193286401865 1105 68-69 0.3141470177832204 1105 70-71 0.32414572045107093 1105 72-73 0.17855104032211955 1105 74-75 0.0755710112629302 1105 76-77 0.11912267482708927 1105 78-79 0.15248698138644556 1105 80-81 0.14989123694169848 1105 82-83 0.21938695409832576 1105 84-85 0.1243646305574515 1105 86-87 0.1267788446929643 1105 88-89 0.17480803665003108 1105 90-91 0.30337425196123036 1105 92-93 0.31022794167463985 1105 94-95 0.19608517410369686 1105 96-97 0.16098337139667152 1105 98-99 0.254621934214466 1105 100-101 0.6307670868625017 1106 1 -0.02778481484362061 1106 2 -0.04986242826727505 1106 3 -0.018516602630690215 1106 4 -0.09188110805825289 1106 5 0.027280576422135994 1106 6 -0.009329740759874028 1106 7 0.06730558401039843 1106 8 -8.79339025395609E-4 1106 9 0.006487320607945435 1106 10-11 0.05027653551593403 1106 12-13 0.0337310723246631 1106 14-15 0.043975772034528404 1106 16-17 0.13717363718480158 1106 18-19 0.12959861924019833 1106 20-21 0.1471498447792996 1106 22-23 -0.017370743296353908 1106 24-25 0.10690194284111243 1106 26-27 -0.0991805903317271 1106 28-29 0.13064231543499005 1106 30-31 0.11629312861413155 1106 32-33 0.1930402056765672 1106 34-35 0.08518299621305658 1106 36-37 0.10030070432455318 1106 38-39 0.22822058359789565 1106 40-41 0.10342176825496097 1106 42-43 -0.07626551236574386 1106 44-45 0.0025265973303731926 1106 46-47 -0.1442157341840158 1106 48-49 -0.11538573802440766 1106 50-51 -0.08249411039243881 1106 52-53 -0.16409822811171892 1106 54-55 -0.04864840652552971 1106 56-57 0.05933257560577232 1106 58-59 -0.10761473246419939 1106 60-61 -0.1339492750571054 1106 62-63 -0.23787085699444077 1106 64-65 -0.11038082357513446 1106 66-67 -0.13867763999482463 1106 68-69 0.05924849275204025 1106 70-71 -0.11598456330777651 1106 72-73 -0.020696014378501104 1106 74-75 0.12442484401733367 1106 76-77 0.0027717538734677305 1106 78-79 -0.1557297081279856 1106 80-81 -0.1040654622615449 1106 82-83 -0.011082077740809382 1106 84-85 0.1491845111993797 1106 86-87 0.14477531961025392 1106 88-89 0.22218921971726147 1106 90-91 0.43634305188701106 1106 92-93 0.3978628379320739 1106 94-95 0.4071546553393297 1106 96-97 0.37940579380877537 1106 98-99 0.4205984439702881 1106 100-101 0.9761685030799434 1107 1 -0.11340277243390062 1107 2 0.021404540058519217 1107 3 -0.010487146504353007 1107 4 0.03909662240045009 1107 5 -0.009029422690630895 1107 6 0.003069336518095156 1107 7 0.07923886410976877 1107 8 0.006994851374749089 1107 9 0.04097849264236686 1107 10-11 0.05330421717683009 1107 12-13 0.08845115040114848 1107 14-15 0.19551512080570888 1107 16-17 0.08734442948362187 1107 18-19 0.09433111857477172 1107 20-21 0.08151680423276986 1107 22-23 -0.054567975130268564 1107 24-25 -0.06437833397549753 1107 26-27 0.08443222130876649 1107 28-29 -0.045406837257750965 1107 30-31 -0.04998537373704437 1107 32-33 -0.09608675732583549 1107 34-35 -0.01807980211894744 1107 36-37 0.049417907767022484 1107 38-39 0.19604730704745776 1107 40-41 0.26363381687531984 1107 42-43 0.16808946944598802 1107 44-45 0.08312059963718355 1107 46-47 -0.06707084908065042 1107 48-49 0.013839707810035407 1107 50-51 0.06315623119215985 1107 52-53 0.22262433421638406 1107 54-55 0.1480621781608349 1107 56-57 0.10703242477620734 1107 58-59 -0.015098540467036514 1107 60-61 0.11398790886623544 1107 62-63 0.0940872656211198 1107 64-65 0.27441200758447337 1107 66-67 0.09985487708439678 1107 68-69 0.26342292270502554 1107 70-71 0.2669228983194074 1107 72-73 0.33781254741725775 1107 74-75 0.35007030582196563 1107 76-77 0.11314350438362197 1107 78-79 0.1755740803557373 1107 80-81 0.2909859972362767 1107 82-83 0.387895386548081 1107 84-85 0.1609181639365005 1107 86-87 0.2946954686653456 1107 88-89 0.48176024269659123 1107 90-91 0.28309578012763126 1107 92-93 0.19812457158480967 1107 94-95 0.14227132644215956 1107 96-97 0.16570470251253155 1107 98-99 -0.019302629581307684 1107 100-101 0.6643727440523541 1108 1 -0.09905415470341694 1108 2 0.004324752168521684 1108 3 -0.004107162028134326 1108 4 -0.016843400667603703 1108 5 -0.05604327077518434 1108 6 -0.09993894041457452 1108 7 0.04330565989948809 1108 8 0.0015941709397893078 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-1.3664891476339278 2316 94-95 -1.4039411232157697 2316 96-97 -1.349011405040212 2316 98-99 -1.6634520429275064 2316 100-101 -1.8715408843666133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 7.0 6 18.0 7 56.0 8 132.0 9 218.0 10 446.0 11 711.0 12 1201.0 13 1916.0 14 2269.0 15 3193.0 16 4320.0 17 6359.0 18 5549.0 19 2780.0 20 2333.0 21 2387.0 22 2452.0 23 2868.0 24 3336.0 25 4125.0 26 5005.0 27 6382.0 28 8388.0 29 10886.0 30 13771.0 31 17399.0 32 21921.0 33 28419.0 34 38612.0 35 58337.0 36 89877.0 37 192571.0 38 346075.0 39 79886.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.98379289008595 24.172251888749656 15.073496149706484 21.77045907145791 2 13.333734437337528 24.73584189310025 40.67485057146947 21.25557309809275 3 16.88703499931032 30.44681757982367 31.97779757503223 20.688349845833777 4 9.704745498688576 19.484162830228403 45.12004015685233 25.69105151423069 5 8.94402620151646 42.57435292189845 38.26433621407585 10.217284662509243 6 24.416444811811424 44.70778941298605 18.75175660301239 12.124009172190135 7 24.26139704776612 34.97016756566714 26.085049143399996 14.68338624316675 8 22.4337002278528 36.446491927663736 22.960447782091 18.159360062392466 9 24.437705541998238 18.539356722902163 25.365191932538146 31.65774580256145 10-11 21.17543408413485 29.052627321320784 31.450901978191563 18.3210366163528 12-13 23.15161094275361 27.777795062942197 30.43818928914416 18.632404705160035 14-15 20.603721768602362 30.012580129617856 28.856203829213072 20.52749427256671 16-17 17.62715538251882 32.950724631668905 30.131277911335037 19.290842074477244 18-19 17.11924365730192 33.56208496202626 31.761301115203082 17.557370265468737 20-21 18.441618782464765 31.484824238489512 31.16145355020672 18.912103428839004 22-23 18.52323928653101 30.728201128686084 31.37017180234563 19.37838778243728 24-25 19.71919653390514 31.421778214525553 30.857941448717717 18.001083802851596 26-27 18.204587965212486 30.855380620029216 33.049850315781065 17.89018109897723 28-29 17.810929226416455 31.410934204665136 32.8119482116938 17.966188357224606 30-31 18.502020575489354 30.7950431728506 31.66876770563951 19.03416854602053 32-33 17.8219731736989 32.12373328023248 32.25202576635833 17.80226777971029 34-35 19.036134082071104 30.69589779304201 32.202690208105054 18.06527791678183 36-37 19.106768849591667 29.600241024207683 31.929795995409755 19.363194130790895 38-39 17.703120770531942 31.977189781968168 32.42112512017523 17.89856432732466 40-41 17.2167280899762 31.521955008786982 33.40479075886438 17.856526142372438 42-43 17.526368220091342 31.44242033268633 30.67171481717913 20.359496630043203 44-45 17.916532916950374 30.589627521517066 31.356447704336293 20.13739185719627 46-47 19.361626098397995 30.723870406280838 31.7832802340483 18.131223261272858 48-49 18.462651539708265 31.300252836304697 31.503040518638574 18.73405510534846 50-51 17.38025846332559 30.7598842515765 32.3291258712247 19.530731413873216 52-53 18.043518395592358 32.12440257886789 30.951808028676215 18.880270996863537 54-55 18.498895995403526 30.63988707936077 31.04601972377587 19.815197201459835 56-57 19.399400738653394 30.6868895763996 30.177185545957368 19.736524138989637 58-59 19.475349342747666 29.979407873960216 31.107126244615372 19.43811653867674 60-61 19.11324689925254 32.28811775113747 30.77811415231025 17.820521197299737 62-63 18.043566866033547 32.00595507866989 31.95446262726621 17.996015428030354 64-65 17.97009461137774 31.918337079933522 31.1355698851129 18.975998423575838 66-67 18.83311778755656 31.51783982684432 29.474113246057172 20.17492913954195 68-69 17.8021279398145 31.172119611830922 30.978646967130913 20.047105481223664 70-71 19.986911680288234 30.097534946695177 31.414352659692796 18.501200713323797 72-73 19.138192014046798 29.8334742956766 31.693969802080574 19.334363888196034 74-75 17.99274853534311 32.58989918265456 29.97576276758703 19.441589514415295 76-77 18.000788715241065 30.71957536153345 31.58762974443652 19.692006178788965 78-79 17.597725052352242 30.7264840576129 31.667682391892747 20.008108498142104 80-81 19.37210675906715 31.337050026005787 29.619371743767037 19.671471471160025 82-83 19.650826212717234 30.14444851221559 31.0519187031162 19.15280657195098 84-85 17.750090229119735 30.717777833090647 31.092850949791124 20.43928098799849 86-87 18.15524357374763 31.66180936641178 31.01247321475394 19.17047384508665 88-89 18.387348355813145 31.067528124763616 30.277556790764276 20.26756672865896 90-91 18.409239993570335 29.440540924363496 31.498606645341955 20.65161243672422 92-93 20.096036467226984 31.307763485233302 30.408461861341106 18.187738186198608 94-95 18.981313907070472 29.261620858064724 31.614313731794365 20.142751503070443 96-97 18.182162323900023 29.730255307696257 32.175625227198594 19.911957141205125 98-99 18.31466215301708 31.10316313285863 30.4888110339649 20.09336368015939 100-101 17.72682205402535 29.951234891839363 31.3784076978762 20.943535356259087 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 979.0 1 613.0 2 424.0 3 567.5 4 372.0 5 446.0 6 1026.0 7 1654.0 8 1938.0 9 1681.0 10 1182.0 11 895.5 12 841.0 13 815.0 14 783.5 15 768.5 16 770.5 17 818.0 18 906.0 19 982.0 20 1133.5 21 1369.0 22 1679.0 23 2072.5 24 2280.5 25 3030.5 26 5690.5 27 8593.5 28 8720.0 29 10062.5 30 15958.5 31 21541.0 32 30514.5 33 41762.0 34 49630.5 35 57379.0 36 70162.0 37 78936.5 38 72710.0 39 64480.5 40 55393.0 41 44132.0 42 33536.0 43 32733.0 44 35642.5 45 31988.5 46 25229.0 47 27245.0 48 43095.0 49 37677.5 50 17993.5 51 7647.0 52 2686.0 53 1148.5 54 446.5 55 315.0 56 225.5 57 194.5 58 194.5 59 195.0 60 204.0 61 168.5 62 100.0 63 85.0 64 54.5 65 28.0 66 30.0 67 26.0 68 20.0 69 16.0 70 20.0 71 24.5 72 15.0 73 9.5 74 7.0 75 3.5 76 4.0 77 4.0 78 1.5 79 0.5 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.270063128812023 2 0.02209041721849497 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.185543948003513E-4 12-13 1.0371087896007029E-4 14-15 0.0 16-17 5.185543948003513E-4 18-19 0.0 20-21 5.1855439480035145E-5 22-23 5.1855439480035145E-5 24-25 0.002229783897641511 26-27 0.0023334947766015814 28-29 0.002540916534521722 30-31 0.0014000968659609488 32-33 0.0016593740633611246 34-35 0.0019186512607612999 36-37 5.1855439480035145E-5 38-39 2.0742175792014058E-4 40-41 8.815424711605973E-4 42-43 0.0038891579610026354 44-45 0.004407712355802987 46-47 0.014986222009730156 48-49 0.016230752557250996 50-51 0.006118941858644147 52-53 0.007778315922005271 54-55 2.0742175792014058E-4 56-57 0.0033187481267222493 58-59 0.0016075186238810894 60-61 0.0016593740633611246 62-63 0.0 64-65 0.0011926751080408083 66-67 0.0 68-69 0.0 70-71 5.1855439480035145E-5 72-73 0.0014000968659609488 74-75 0.0 76-77 0.19648026018985312 78-79 0.10702962708679252 80-81 0.0010889642290807379 82-83 0.0 84-85 7.25976152720492E-4 86-87 0.12813479095516683 88-89 0.5980487835232452 90-91 0.3162663253887343 92-93 0.302576489366005 94-95 0.002696482852961827 96-97 0.0030594709293220736 98-99 0.003370603566202284 100-101 5.185543948003513E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 964219.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.373285529532192 #Duplication Level Percentage of deduplicated Percentage of total 1 69.6013454452915 6.523932840983221 2 9.981300965932352 1.8711516781975877 3 3.837174564887861 1.0790079847005711 4 2.2416711846778568 0.8404729630924096 5 1.4671549806924173 0.6876031275052659 6 1.1230484957788867 0.6315992528668279 7 0.8641387933037542 0.5669873752747041 8 0.740216200666084 0.5550606241942961 9 0.6041226391086425 0.5096352592097854 >10 6.055610263446154 12.692655921528202 >50 1.4228969118932495 9.425763234285988 >100 1.7537259761670299 34.56693966827038 >500 0.22018389227586055 13.956787825172498 >1k 0.0840903307184191 13.536136500110452 >5k 0.0022129034399583974 1.1958901452885704 >10k+ 0.0011064517199791987 1.3603755993192417 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13117 1.3603755993192417 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6241 0.6472595955897986 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5290 0.5486305496987718 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4317 0.44771986447062334 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3955 0.4101765262870779 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3608 0.37418885128793355 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3229 0.3348824281620669 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2939 0.3048062732636465 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2875 0.29816877701020206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2864 0.2970279573416413 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2778 0.2881088217510752 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 2673 0.27721917946026786 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2540 0.2634256325585785 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2479 0.25709926894201424 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2439 0.2529508337836114 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2344 0.24309830028240473 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 2336 0.24226861325072416 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 2320 0.24060923918736302 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2224 0.23065299480719628 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 2183 0.2264008487698334 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 2116 0.21945221987950872 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 2115 0.21934850900054864 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1982 0.2055549620988593 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1943 0.20151023781941657 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1920 0.19912488760333494 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1900 0.19705067002413354 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1895 0.1965321156293332 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1880 0.1949764524449321 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1746 0.1810791946642827 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1706 0.1769307595058799 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1700 0.17630849423211947 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1659 0.1720563481947566 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1658 0.17195263731579652 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1622 0.168219045673234 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1615 0.16749306952051352 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1588 0.1646928757885916 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 1587 0.16458916490963155 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1581 0.1639668996358711 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1555 0.1612704167829093 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1542 0.1599221753564284 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1539 0.15961104271954815 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1457 0.1511067506448224 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1454 0.1507956180079422 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 1429 0.14820284603394043 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 1427 0.1479954242760203 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1390 0.1441581217544977 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1390 0.1441581217544977 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1372 0.14229132593321642 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1352 0.14021710835401502 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 1283 0.13306105770577017 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 1278 0.1325425033109698 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1244 0.12901633342632743 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1241 0.12870520078944722 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 1229 0.12746067024192637 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1216 0.12611242881544546 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1208 0.1252827417837649 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 1204 0.12486789826792462 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 1189 0.12331223508352356 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 1188 0.12320852420456348 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 1186 0.12300110244664335 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 1176 0.12196399365704265 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 1171 0.12144543926224229 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 1156 0.11988977607784125 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1150 0.11926751080408081 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 1134 0.1176081367407197 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 1130 0.11719329322487941 No Hit ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT 1122 0.11636360619319884 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 1117 0.1158450517983985 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 1115 0.11563763004047835 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1108 0.11491165388775786 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1098 0.11387454509815716 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 1095 0.11356341246127695 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 1072 0.11117806224519534 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1055 0.10941497730287415 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 1037 0.10754818148159288 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1036 0.1074444706026328 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1026 0.1064073618130321 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 1018 0.10557767478135154 No Hit GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG 1013 0.1050591203865512 No Hit TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA 991 0.10277748104942964 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 985 0.10215521577566923 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 981 0.10174037225982895 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 977 0.10132552874398866 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 974 0.10101439610710845 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 966 0.10018470907542788 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.185543948003514E-5 0.0 12-13 0.0 0.0 0.0 2.0742175792014055E-4 0.0 14-15 0.0 0.0 0.0 5.704098342803865E-4 0.0 16-17 0.0 0.0 0.0 0.0019186512607613 0.0 18-19 0.0 0.0 0.0 0.0031631818082821436 0.0 20-21 0.0 0.0 0.0 0.006015230979684076 0.0 22-23 0.0 0.0 0.0 0.01332684794636903 0.0 24-25 0.0 0.0 0.0 0.019808777881373422 0.0 26-27 0.0 0.0 0.0 0.02380164672133613 0.0 28-29 0.0 0.0 0.0 0.028987190669339642 0.0 30-31 0.0 0.0 0.0 0.04195105053934843 0.0 32-33 0.0 0.0 0.0 0.06829361379520626 0.0 34-35 0.0 0.0 0.0 0.10495540950759112 0.0 36-37 0.0 0.0 0.0 0.15966289815902818 0.0 38-39 0.0 0.0 0.0 0.23573482787623973 0.0 40-41 0.0 0.0 0.0 0.3191702299996163 0.0 42-43 0.0 0.0 0.0 0.401412957014952 0.0 44-45 0.0 0.0 0.0 0.49527130247381557 0.0 46-47 0.0 0.0 0.0 0.6164574645386578 0.0 48-49 0.0 0.0 0.0 0.739199289787901 0.0 50-51 0.0 0.0 0.0 0.8669192372272274 0.0 52-53 0.0 0.0 0.0 0.9908018821450313 0.0 54-55 0.0 0.0 0.0 1.1206479026030394 0.0 56-57 0.0 0.0 0.0 1.2935339378294763 0.0 58-59 0.0 0.0 0.0 1.459886187681429 0.0 60-61 0.0 0.0 0.0 1.6409135269062318 0.0 62-63 0.0 0.0 0.0 1.8051397037395032 0.0 64-65 0.0 0.0 0.0 1.989278369333108 0.0 66-67 0.0 0.0 0.0 2.2157829289819015 0.0 68-69 0.0 0.0 0.0 2.451414045979181 0.0 70-71 0.0 0.0 0.0 2.7234995369309254 0.0 72-73 0.0 0.0 0.0 2.9696054527031723 0.0 74-75 0.0 0.0 0.0 3.236246122509513 0.0 76-77 0.0 0.0 0.0 3.5246660768974682 0.0 78-79 0.0 0.0 0.0 3.8987512173064416 0.0 80-81 0.0 0.0 0.0 4.29409708790223 0.0 82-83 0.0 0.0 0.0 4.638417206049663 0.0 84-85 0.0 0.0 0.0 5.001560848728349 0.0 86-87 0.0 0.0 0.0 5.38663934230709 0.0 88-89 0.0 0.0 0.0 5.828292120358549 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGATG 20 0.002085646 71.14927 5 TGGATGA 25 0.0050492254 56.919415 6 ATGTATT 55 8.1112194E-7 51.89023 1 GGGCATC 55 5.0043185E-5 43.12077 7 GATTGGC 45 9.691545E-4 42.16253 9 CATTCCG 215 0.0 41.9174 9 TAGGCAT 240 0.0 41.50374 5 CTCCCGC 185 0.0 41.023 7 ACTCCCG 185 0.0 41.023 6 CCTTTAG 235 0.0 40.48174 1 TACTCCC 190 0.0 39.94345 5 GGCATTC 240 0.0 39.52737 7 GTATGGT 85 3.600435E-7 39.172035 1 CTTTAGG 250 0.0 37.954147 2 GGGACCA 50 0.0016269549 37.946278 7 GGCATCT 65 1.3391257E-4 36.486805 8 TGGGTAT 315 0.0 36.139313 6 GATGACC 465 0.0 35.70214 9 GTGGTAC 200 0.0 35.674534 1 GGTACTC 215 0.0 35.298862 3 >>END_MODULE