FastQCFastQC Report
Thu 26 May 2016
SRR936326_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936326_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530055
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30570.19979673933289982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20980.13711925388303037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39500.042.5383071
GGTATCA22750.037.4489861
ATCAACG49000.033.9979673
TCAACGC49650.033.362244
TATCAAC50250.033.152252
CAACGCA50000.033.0340465
AACGCAG51550.032.222296
GTACATG60350.031.5280931
TACATGG60200.030.8960132
GTACCTG14400.030.5681691
ACATGGG61900.028.7477283
TACCTGG14750.028.556462
ACGCAGA60950.027.0198867
CGCAGAG61500.026.7012948
GTGTAGC8050.024.1066531
CATGGGA27200.024.0113494
GCAGAGT67450.023.2233059
CATGGGG37950.023.1957024
GTATAGG4500.023.1397721
GTAACGT2100.021.41180226-27
Produced by FastQC (version 0.11.5)