FastQCFastQC Report
Thu 26 May 2016
SRR936326_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936326_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530055
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.1806471009212087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22690.14829532271715723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.1016956906777861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22800.044.4313661
GTATCAA37350.038.5830841
TCAACGC46750.030.4402124
ATCAACG46800.030.4076923
TATCAAC47750.029.806622
CAACGCA47900.029.7093935
GTACATG59750.029.6107861
TACATGG59750.029.3784332
GTACCTG15800.028.8974191
AACGCAG50150.028.4710586
GTATACG856.360761E-427.9766671
TACCTGG16200.027.2353362
ACATGGG62200.026.7685453
GTATAGG4550.025.0867711
ACGCAGA60850.023.2307787
CATGGGG35550.022.8173164
CGCAGAG62550.022.6752418
GCAGAGT68400.020.1811059
CATGGGA28050.020.1243634
GTGGTAT13750.019.7159211
Produced by FastQC (version 0.11.5)