##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936326_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1530055 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.449255092137214 34.0 31.0 34.0 31.0 34.0 2 32.58759848502178 34.0 31.0 34.0 31.0 34.0 3 32.67129612987768 34.0 31.0 34.0 31.0 34.0 4 36.166431272078455 37.0 35.0 37.0 35.0 37.0 5 36.12272957508063 37.0 35.0 37.0 35.0 37.0 6 36.15832960253063 37.0 36.0 37.0 35.0 37.0 7 36.10354856524766 37.0 36.0 37.0 35.0 37.0 8 36.079150749482864 37.0 36.0 37.0 35.0 37.0 9 37.84085408694459 39.0 38.0 39.0 35.0 39.0 10-11 37.77321011336194 39.0 38.0 39.0 35.0 39.0 12-13 37.717265719206175 39.0 38.0 39.0 35.0 39.0 14-15 39.06331635137299 40.0 38.0 41.0 36.0 41.0 16-17 38.98633121031597 40.0 38.0 41.0 35.0 41.0 18-19 38.99724944528138 40.0 38.5 41.0 35.0 41.0 20-21 38.94798716386012 40.0 38.0 41.0 35.0 41.0 22-23 38.83375368859289 40.0 38.0 41.0 35.0 41.0 24-25 38.98800664028417 40.0 38.0 41.0 35.0 41.0 26-27 38.915177232191 40.0 38.0 41.0 35.0 41.0 28-29 38.82342432134793 40.0 38.0 41.0 35.0 41.0 30-31 38.71857286175987 40.0 38.0 41.0 35.0 41.0 32-33 38.57658319472176 40.0 38.0 41.0 34.0 41.0 34-35 38.44259487404048 40.0 38.0 41.0 34.0 41.0 36-37 38.29418909777753 40.0 38.0 41.0 34.0 41.0 38-39 38.16688288983076 40.0 38.0 41.0 33.0 41.0 40-41 38.02661211525076 40.0 38.0 41.0 33.0 41.0 42-43 38.061516089290905 40.0 38.0 41.0 33.0 41.0 44-45 37.93832607324573 40.0 38.0 41.0 33.0 41.0 46-47 37.74056945665352 40.0 37.0 41.0 33.0 41.0 48-49 37.51894409024512 40.0 37.0 41.0 32.5 41.0 50-51 37.316681099699025 40.0 37.0 41.0 32.0 41.0 52-53 37.09678279538971 40.0 36.0 41.0 31.5 41.0 54-55 36.897867396923644 39.5 36.0 41.0 31.0 41.0 56-57 36.635285659665826 39.0 35.0 41.0 31.0 41.0 58-59 36.36099682691145 39.0 35.0 41.0 30.5 41.0 60-61 36.07870109244439 38.5 35.0 40.5 30.0 41.0 62-63 35.77683939466228 38.0 35.0 40.0 30.0 41.0 64-65 35.39074543071981 37.0 34.5 40.0 29.0 41.0 66-67 35.037423164526764 37.0 34.0 39.5 29.0 41.0 68-69 34.6855073837215 36.0 34.0 39.0 28.5 41.0 70-71 34.306593227040864 36.0 34.0 39.0 28.0 40.5 72-73 33.89607955269582 35.0 34.0 38.0 28.0 40.0 74-75 33.465216283074795 35.0 33.0 37.0 27.0 39.5 76-77 32.75079163820909 35.0 32.5 36.0 26.0 39.0 78-79 32.80186823349487 35.0 33.0 36.0 26.0 39.0 80-81 32.63478175621138 35.0 33.0 36.0 26.0 37.5 82-83 32.35406210887844 35.0 33.0 35.5 26.0 37.0 84-85 32.068757985824035 35.0 33.0 35.0 26.0 37.0 86-87 31.654600978396203 35.0 32.0 35.0 25.0 36.0 88-89 31.17051936041515 34.5 32.0 35.0 23.5 36.0 90-91 30.948140426324542 34.0 32.0 35.0 22.5 36.0 92-93 30.663550983461377 34.0 31.0 35.0 20.0 35.0 94-95 30.498194182562067 34.0 31.0 35.0 19.5 35.0 96-97 30.229474103872086 34.0 31.0 35.0 17.5 35.0 98-99 29.761259562564746 34.0 31.0 35.0 2.0 35.0 100-101 26.383376741358973 31.0 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21521273040471556 1101 2 0.23200693520174553 1101 3 0.23813561991636334 1101 4 0.19430091872542476 1101 5 0.1626079981272497 1101 6 0.236280809177579 1101 7 0.22884979759278679 1101 8 0.10886931738212269 1101 9 0.26748227054557816 1101 10-11 0.19510963620160737 1101 12-13 0.32809655121143066 1101 14-15 0.39438928683190966 1101 16-17 0.4580307923651432 1101 18-19 0.4035241508631131 1101 20-21 0.33612379397667524 1101 22-23 0.44134984533150856 1101 24-25 0.15987612088859038 1101 26-27 0.31966537133198614 1101 28-29 0.4478748729832702 1101 30-31 0.4024505072916327 1101 32-33 0.4283817776235068 1101 34-35 0.41032368350249016 1101 36-37 0.3626068859747349 1101 38-39 0.332048588517587 1101 40-41 0.3509384585841602 1101 42-43 0.4027071825395865 1101 44-45 0.45733078739669253 1101 46-47 0.4754989615765979 1101 48-49 0.5951638204452223 1101 50-51 0.46101220914594165 1101 52-53 0.47227263355477334 1101 54-55 0.6015050171687264 1101 56-57 0.42089792478125787 1101 58-59 0.11892620672770704 1101 60-61 0.25791837660301553 1101 62-63 0.1964591713901669 1101 64-65 0.1798520679896285 1101 66-67 0.27716352201802863 1101 68-69 0.37480980865170466 1101 70-71 0.5458377471365665 1101 72-73 0.49078616230413985 1101 74-75 0.4201648253709038 1101 76-77 0.32313763526539674 1101 78-79 0.29383122417807783 1101 80-81 0.4517369278540144 1101 82-83 0.43721812028488216 1101 84-85 0.4904145928283512 1101 86-87 0.5347085899031434 1101 88-89 0.5957736653090073 1101 90-91 0.6180069493846858 1101 92-93 0.6752020812626505 1101 94-95 0.6132427378538914 1101 96-97 0.4978437555550599 1101 98-99 0.793365440890117 1101 100-101 1.197858462744957 1102 1 0.01338570422301899 1102 2 0.1150934958214691 1102 3 0.12409539884440335 1102 4 0.08619987746394031 1102 5 0.07374143269641564 1102 6 0.1022500808324267 1102 7 0.05533759326308996 1102 8 0.10709478508319847 1102 9 0.19835918235137484 1102 10-11 0.1467853813881277 1102 12-13 0.2127382555593016 1102 14-15 0.23587145709038992 1102 16-17 0.23947694405055842 1102 18-19 0.2876942071371502 1102 20-21 0.22531687733486905 1102 22-23 0.23583056677875192 1102 24-25 0.17015451278281546 1102 26-27 0.24107993703973563 1102 28-29 0.2685930729710151 1102 30-31 0.2410430237756529 1102 32-33 0.3673436936601888 1102 34-35 0.22539854207032306 1102 36-37 -0.02312329447174477 1102 38-39 0.15366999207650878 1102 40-41 0.024193422966511946 1102 42-43 0.17166536822350054 1102 44-45 0.17773038836349286 1102 46-47 0.1405210025327861 1102 48-49 0.18357915762379662 1102 50-51 0.02950776136017197 1102 52-53 0.05313462892992504 1102 54-55 0.08992772540482719 1102 56-57 0.29539172487518783 1102 58-59 0.05979015443703162 1102 60-61 0.20180645008249343 1102 62-63 0.14071729188007254 1102 64-65 0.21779184744559643 1102 66-67 0.26054851085933706 1102 68-69 0.18524837163415242 1102 70-71 0.21916617307213215 1102 72-73 0.1481039539950686 1102 74-75 0.20571262194764728 1102 76-77 0.07279684836516509 1102 78-79 0.07762540632189285 1102 80-81 0.19114065910326872 1102 82-83 0.1627602789986895 1102 84-85 0.21474700162521287 1102 86-87 0.147850836870429 1102 88-89 0.15041038423215625 1102 90-91 0.2914133192733175 1102 92-93 0.37209921466833507 1102 94-95 0.1994064161709801 1102 96-97 0.01953881988496775 1102 98-99 -0.056330229061817505 1102 100-101 0.4680943814103138 1103 1 0.128646830712988 1103 2 0.15323680486415725 1103 3 0.14120931464286457 1103 4 0.06087274396581677 1103 5 0.0813471784916473 1103 6 0.12765179014971295 1103 7 0.1848259093065181 1103 8 0.1217639968760551 1103 9 0.1828360527351478 1103 10-11 0.2485490254293481 1103 12-13 0.19169972554253434 1103 14-15 0.1659353898042326 1103 16-17 0.33181440210165647 1103 18-19 0.22752139866612708 1103 20-21 0.30674996776175334 1103 22-23 0.25771758194332506 1103 24-25 0.24837590418980682 1103 26-27 0.33421871473711207 1103 28-29 0.28234638505106346 1103 30-31 0.25105396320872586 1103 32-33 0.3559948343985937 1103 34-35 0.3360584392939785 1103 36-37 0.3202830424973655 1103 38-39 0.3332278216732405 1103 40-41 0.4265741695642973 1103 42-43 0.31141798393331044 1103 44-45 0.32633159787462773 1103 46-47 0.42578042513814296 1103 48-49 0.48314009866197694 1103 50-51 0.4298991609408702 1103 52-53 0.4861428248618438 1103 54-55 0.39372491868708437 1103 56-57 0.5021447875460439 1103 58-59 0.5429268825340827 1103 60-61 0.3590848771747517 1103 62-63 0.3380283644136952 1103 64-65 0.4143238249148027 1103 66-67 0.3189361915266744 1103 68-69 0.4071939115309746 1103 70-71 0.16007718337692012 1103 72-73 0.3514304800212926 1103 74-75 0.3669188734755835 1103 76-77 0.4752148828725282 1103 78-79 0.4064210562303998 1103 80-81 0.5435148617963961 1103 82-83 0.4914062833892814 1103 84-85 0.43011283058977057 1103 86-87 0.3931450285994309 1103 88-89 0.4361273862842232 1103 90-91 0.36352863028164606 1103 92-93 0.5432704748268193 1103 94-95 0.5316560130417578 1103 96-97 0.44410735432195736 1103 98-99 0.21770034702403862 1103 100-101 0.5669853747750437 1104 1 -0.10877159576014606 1104 2 -0.004806970522295728 1104 3 -0.01469766747351997 1104 4 0.02695885095948114 1104 5 0.0358681325409691 1104 6 -0.03110328714060273 1104 7 -0.035443307395119916 1104 8 0.03621718955320574 1104 9 0.0015589818744601303 1104 10-11 -0.033214599034046444 1104 12-13 0.0463150065019704 1104 14-15 0.08938332712497044 1104 16-17 0.11465412335601144 1104 18-19 0.12103112973817787 1104 20-21 0.11298348461546937 1104 22-23 0.08892077943307442 1104 24-25 0.11037852224281863 1104 26-27 0.1963977144868636 1104 28-29 0.23319171779404968 1104 30-31 0.16097657664165155 1104 32-33 0.02383633631400528 1104 34-35 0.06638574404689734 1104 36-37 0.012015962589003948 1104 38-39 0.15204772465127547 1104 40-41 0.33896396108257676 1104 42-43 0.3077486190036751 1104 44-45 0.32826794646068436 1104 46-47 0.31360022223903883 1104 48-49 0.18449099476762854 1104 50-51 0.2903641541069888 1104 52-53 0.39990805673278373 1104 54-55 0.29291207097691796 1104 56-57 0.26143839449158435 1104 58-59 0.3591727677632619 1104 60-61 0.2440974861800811 1104 62-63 0.24640132146607385 1104 64-65 0.07235749384660295 1104 66-67 0.029614864019791298 1104 68-69 0.253163842422083 1104 70-71 0.2747649901800102 1104 72-73 -0.044341893744281435 1104 74-75 -0.048222520637644095 1104 76-77 -0.041948042801713825 1104 78-79 -0.08841093599244232 1104 80-81 0.10249276234887361 1104 82-83 0.0879145033057327 1104 84-85 0.12566863746985746 1104 86-87 0.27848778179017586 1104 88-89 0.39723486121058826 1104 90-91 0.5610657835339978 1104 92-93 0.5457104617365545 1104 94-95 0.2905318865281963 1104 96-97 0.1977865850763827 1104 98-99 0.12330413550075647 1104 100-101 -0.041930443270270956 1105 1 0.08017606029100222 1105 2 0.10645012271162102 1105 3 0.0626766007442967 1105 4 0.032552960767979755 1105 5 0.0406967719875837 1105 6 0.07532890977805096 1105 7 0.08966524341414583 1105 8 0.17795834453381332 1105 9 0.18179075656789934 1105 10-11 0.12249405059388607 1105 12-13 0.06642872283251222 1105 14-15 0.1235525016896375 1105 16-17 0.17020967891156857 1105 18-19 0.15090506107417667 1105 20-21 0.2249265066700019 1105 22-23 0.12867189862934936 1105 24-25 0.12000772765089351 1105 26-27 0.13689970583273947 1105 28-29 0.035540345562097286 1105 30-31 0.174979631036841 1105 32-33 0.18064640234283758 1105 34-35 0.1175141109935538 1105 36-37 0.2837654273212138 1105 38-39 0.2803145425404452 1105 40-41 0.3933376229131156 1105 42-43 0.23366924216018248 1105 44-45 0.07283759864891692 1105 46-47 0.06183346423996028 1105 48-49 0.1871373886348806 1105 50-51 0.10795762009689014 1105 52-53 0.07807081557032092 1105 54-55 0.04647366046021517 1105 56-57 0.017427404657929912 1105 58-59 0.09469807881747982 1105 60-61 0.07966172580037778 1105 62-63 0.21588357153719784 1105 64-65 0.06869858294810172 1105 66-67 0.10276313693898231 1105 68-69 0.20506460452362063 1105 70-71 0.08366409781749695 1105 72-73 0.02908243511226516 1105 74-75 0.10771446000287455 1105 76-77 -0.0864866269997151 1105 78-79 -0.11255881213971008 1105 80-81 0.10096743632213645 1105 82-83 0.18957701509659586 1105 84-85 0.02763509152666188 1105 86-87 0.049494234948632254 1105 88-89 -0.00813316346413373 1105 90-91 0.14328085637533405 1105 92-93 0.12907186855504094 1105 94-95 0.07871369790432325 1105 96-97 0.023424040345961572 1105 98-99 0.04318428043480438 1105 100-101 0.3660465439581664 1106 1 -0.009424094461401467 1106 2 -0.02086395585126155 1106 3 -0.05384758954907909 1106 4 -0.05461012259929987 1106 5 -0.04276350667834805 1106 6 0.012647210552003685 1106 7 0.08209130357042227 1106 8 0.004495685061648658 1106 9 -0.09226887146832752 1106 10-11 -0.00391188058965497 1106 12-13 -0.10297191849717535 1106 14-15 0.12155322895154796 1106 16-17 0.04902268890165118 1106 18-19 0.1167377015556994 1106 20-21 0.0864012487338286 1106 22-23 -0.050037602089545885 1106 24-25 0.05749133635794834 1106 26-27 -0.19634597477094218 1106 28-29 -0.03909234997556865 1106 30-31 0.03218984692760074 1106 32-33 0.08413795699070903 1106 34-35 0.15503877930818533 1106 36-37 0.06416371946474442 1106 38-39 -5.305952267065095E-5 1106 40-41 -0.3045599205784484 1106 42-43 -0.1923511565132614 1106 44-45 -0.008435081785499676 1106 46-47 0.03962802856172232 1106 48-49 -0.024238912671030732 1106 50-51 -0.02514688613691618 1106 52-53 -0.054567526942136624 1106 54-55 0.16570136783681022 1106 56-57 0.08178985078753698 1106 58-59 0.1836707144257801 1106 60-61 -0.024687802595380504 1106 62-63 -0.10866774878726204 1106 64-65 0.04434972415408822 1106 66-67 0.0678343379255324 1106 68-69 0.07733899988253512 1106 70-71 0.03421238075399913 1106 72-73 -0.09129824278510767 1106 74-75 0.08701051325854081 1106 76-77 0.1621246468618196 1106 78-79 0.03915597285302397 1106 80-81 0.18874478629879832 1106 82-83 0.2710570860743857 1106 84-85 0.3178497918891807 1106 86-87 0.3020452316322455 1106 88-89 0.31699638587528867 1106 90-91 0.5669890364907673 1106 92-93 0.5061936691141575 1106 94-95 0.422118492624886 1106 96-97 0.47703621630985893 1106 98-99 0.43059376794765925 1106 100-101 0.6651257950457996 1107 1 -0.06239341679563637 1107 2 0.05527442358846457 1107 3 -0.023584374285150034 1107 4 0.0031474258132746513 1107 5 0.02600343447605269 1107 6 0.08349816170611746 1107 7 -0.0497705834923039 1107 8 0.06716785226058164 1107 9 0.04242166480417353 1107 10-11 0.07245429966104666 1107 12-13 0.12740941026250852 1107 14-15 0.15041412201890836 1107 16-17 0.19093891992988432 1107 18-19 0.1228559904335853 1107 20-21 0.08251516285634608 1107 22-23 0.10690245984287827 1107 24-25 0.15000589968828848 1107 26-27 0.2184609309738974 1107 28-29 0.2422916207836252 1107 30-31 0.02002062962669271 1107 32-33 0.11623901041024709 1107 34-35 0.2196595007558031 1107 36-37 0.1899423450861093 1107 38-39 0.2696390958502164 1107 40-41 0.21610504223613702 1107 42-43 0.13896699695786197 1107 44-45 0.19440218956249566 1107 46-47 0.11198564938066369 1107 48-49 0.049279267548314465 1107 50-51 0.2627156906968722 1107 52-53 0.27563440255522664 1107 54-55 0.2794503960091532 1107 56-57 0.23757866130968353 1107 58-59 0.17146597979412803 1107 60-61 0.2207528743992384 1107 62-63 0.25336260799779353 1107 64-65 0.13123956703032036 1107 66-67 0.15541934069588592 1107 68-69 0.20520677939290977 1107 70-71 0.24167768277133206 1107 72-73 0.24854602964115458 1107 74-75 0.21174076525522167 1107 76-77 0.08247152136120661 1107 78-79 0.1711533156667855 1107 80-81 0.22502806665006858 1107 82-83 0.2397567647527623 1107 84-85 0.2990469169939516 1107 86-87 0.31190116843980675 1107 88-89 0.47270877289955493 1107 90-91 0.25373622217096425 1107 92-93 0.3739339879471153 1107 94-95 0.27090945819168866 1107 96-97 0.19427833721869447 1107 98-99 0.34581291967623073 1107 100-101 0.5189781027170071 1108 1 -0.1931082069079082 1108 2 0.010155122069825495 1108 3 0.046519939844799296 1108 4 0.03065422430229603 1108 5 -0.09346855232076479 1108 6 0.010181141889432865 1108 7 0.07845813334437679 1108 8 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-0.6485143351025826 2316 86-87 -0.7212123334036669 2316 88-89 -0.6418477451533136 2316 90-91 -0.8453326713452398 2316 92-93 -0.8882839404137322 2316 94-95 -0.73327603170004 2316 96-97 -0.5925990921714082 2316 98-99 -0.771312535555758 2316 100-101 -0.7205943533238717 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 8.0 5 29.0 6 44.0 7 125.0 8 267.0 9 463.0 10 780.0 11 1275.0 12 1835.0 13 2731.0 14 3440.0 15 4544.0 16 5789.0 17 7767.0 18 7407.0 19 5164.0 20 4770.0 21 5260.0 22 5521.0 23 6399.0 24 7705.0 25 9368.0 26 11309.0 27 14383.0 28 18130.0 29 22443.0 30 27893.0 31 34986.0 32 43921.0 33 56931.0 34 78559.0 35 116598.0 36 200011.0 37 361138.0 38 391664.0 39 71334.0 40 55.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.94688397567757 18.007547842429776 13.016063575402082 24.02950460649057 2 16.264078913823646 19.014296266759057 39.51652862848663 25.205096190930664 3 20.26515386701785 22.836564698654623 28.17552310211071 28.722758332216813 4 13.149658018829388 14.939920460375609 35.35167036479081 36.55875115600419 5 14.457715572316026 36.92514321380604 33.47624758587109 15.140893628006836 6 35.28951573636242 34.22269134116094 16.23654051651738 14.251252405959264 7 31.33593236844427 30.891896042952705 19.148069840626643 18.62410174797638 8 27.77102783886854 35.48140426324544 18.235684338144704 18.511883559741314 9 26.9715794530262 15.819235256248959 18.970363810451257 38.238821480273586 10-11 26.446046569689507 25.738651848859938 26.24461537556591 21.57068620588464 12-13 26.47269312063496 22.432835703837902 27.38968689993948 23.70478427558766 14-15 23.09930035194813 24.236775802177046 24.962468669426915 27.701455176447904 16-17 23.666299139830347 27.631765560142924 25.411245536901806 23.290689763124924 18-19 23.33628529693377 26.28696354052632 27.334801690135325 23.04194947240459 20-21 24.00192280732484 25.52716259453503 27.36422133982809 23.106693258312042 22-23 23.6922933137349 25.42981312103588 27.314942049449876 23.56295151577934 24-25 23.55223345046684 25.573219443791768 27.54917336074909 23.325373744992305 26-27 23.477880368962257 25.893402770288777 27.311095730058604 23.317621130690362 28-29 23.201041290855567 25.973121990243293 27.57322760950155 23.252609109399593 30-31 23.352343766850083 25.71444350692173 27.44512060819444 23.488092118033745 32-33 23.25093413139995 25.73179889819226 27.557480861498885 23.459786108908904 34-35 23.736177980649416 25.84933708881157 27.205060810839516 23.209424119699495 36-37 23.831805936261073 25.748437636841576 27.06263961925527 23.357116807642083 38-39 23.478312018705243 25.820519230549277 27.331317062649813 23.369851688095668 40-41 23.600632137030995 25.757982756096542 27.33623389593006 23.3051512109424 42-43 23.64603802417058 25.721102705521993 27.060219914960815 23.572639355346617 44-45 23.5421261593595 25.960567806333817 27.055833897333553 23.441472136973132 46-47 23.534942854061335 26.271182563053365 26.774923113628418 23.41895146925689 48-49 23.93986982362621 26.088090479606628 26.658702909930565 23.313336786836597 50-51 23.658791252783956 26.253870576105125 26.543483624705672 23.543854546405246 52-53 23.890050504687387 26.26505189361531 26.39910794878549 23.445789652911813 54-55 23.62720172543381 26.004836443253488 26.6227574262279 23.745204405084802 56-57 23.622725020816805 26.393941493345814 26.580443054769432 23.402890431067952 58-59 23.811767766172746 26.06848011042881 26.562553613952094 23.55719850944635 60-61 23.773688985095774 26.086706456580107 26.78570961729005 23.353894941034067 62-63 23.620098623905676 26.127328756155826 26.818839845626464 23.433732774312034 64-65 23.601801260097773 26.06163891140101 26.686230425348356 23.65032940315286 66-67 23.55176774691106 26.393103515886686 26.469015819692753 23.586112917509503 68-69 23.356023149494625 26.198894810970845 26.79018074513661 23.654901294397916 70-71 23.46481775649168 26.097501755656417 26.72662967234174 23.711050815510166 72-73 23.499391354787054 26.295956798444482 26.494514023349105 23.710137823419362 74-75 23.444320628996998 26.409344762116394 26.401175121155774 23.74515948773083 76-77 23.654091340987538 26.313166667867204 26.310972954460475 23.72176903668478 78-79 23.56589198003883 26.337229937829886 26.381195543902635 23.71568253822865 80-81 23.471581578882873 26.56065619306716 26.38010506278441 23.587657165265558 82-83 23.698494498563775 26.58875007761159 26.252422298544825 23.46033312527981 84-85 23.708295684596997 26.341327119998304 26.055714138659546 23.894663056745156 86-87 23.544223286930826 26.17302983467062 26.52824655056661 23.754500327831952 88-89 23.634625106581318 26.35663144525254 26.30203419937297 23.706709248793175 90-91 23.541386190802587 26.553102829294644 26.360682864690737 23.544828115212027 92-93 23.53956040390427 26.364392867010434 26.334534968973678 23.761511760111617 94-95 23.571875576993147 26.315462681104364 26.303240638618057 23.80942110328443 96-97 23.486578757054243 26.288995852010938 26.317002049321626 23.90742334161319 98-99 23.40163331732042 26.225690597804597 26.22265141191426 24.150024672960726 100-101 23.231545876575353 26.412975776624435 26.26481078081711 24.090667565983097 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 59.0 1 44.0 2 63.0 3 110.0 4 84.0 5 82.5 6 188.5 7 386.5 8 600.0 9 728.5 10 679.0 11 630.5 12 722.0 13 816.5 14 915.0 15 1006.5 16 1051.0 17 1102.5 18 1234.0 19 1349.5 20 1484.5 21 1709.0 22 2006.5 23 2439.0 24 3032.0 25 3708.5 26 4832.0 27 6389.0 28 7835.0 29 9678.0 30 11900.5 31 14551.0 32 17298.5 33 19809.0 34 22578.0 35 25222.5 36 28276.0 37 31337.5 38 33779.5 39 35846.0 40 38118.0 41 41316.5 42 44587.0 43 47878.0 44 51434.5 45 54902.0 46 56979.0 47 58324.0 48 60825.0 49 61712.0 50 60385.0 51 59172.0 52 58833.5 53 60130.0 54 61217.0 55 58594.5 56 53332.5 57 48384.0 58 45211.5 59 41446.0 60 36291.0 61 30776.5 62 25444.5 63 20563.0 64 15637.0 65 11592.0 66 8432.0 67 6771.0 68 4979.0 69 3113.5 70 2314.0 71 1642.5 72 1218.0 73 1023.5 74 765.0 75 548.0 76 336.0 77 151.5 78 76.0 79 27.0 80 15.0 81 6.0 82 2.0 83 2.0 84 2.0 85 3.0 86 3.0 87 1.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2650885098901673 2 0.018626781390211462 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.6142851073981E-4 12-13 6.53571276849525E-5 14-15 0.0 16-17 5.2285702147962E-4 18-19 0.0 20-21 3.267856384247625E-5 22-23 3.267856384247625E-5 24-25 0.0022548209051308616 26-27 0.002222142341288385 28-29 0.0020260709582335277 30-31 0.001176428298329145 32-33 0.00156857106443886 34-35 0.0015358925005963839 36-37 6.53571276849525E-5 38-39 1.6339281921238125E-4 40-41 4.5749989379466756E-4 42-43 0.003561963458829911 44-45 0.004019463352624579 46-47 0.0130714255369905 48-49 0.013561603994627646 50-51 0.005391963034008581 52-53 0.0064049985131253455 54-55 3.267856384247625E-4 56-57 0.0026796422350830527 58-59 0.0012744639898565736 60-61 0.0012417854260140975 62-63 0.0 64-65 9.803569152742875E-4 66-67 0.0 68-69 0.0 70-71 3.267856384247625E-5 72-73 0.0011437497344866689 74-75 0.0 76-77 0.1937185264581992 78-79 0.10375444019986209 80-81 0.0011437497344866689 82-83 0.0 84-85 8.169640960619063E-4 86-87 0.11979961504651794 88-89 0.5827241504390365 90-91 0.3101522494289421 92-93 0.29381296750770397 94-95 0.002124106649760956 96-97 0.002385535160500766 98-99 0.0026142851073981 100-101 2.6142851073981E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1530055.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.654580046373944 #Duplication Level Percentage of deduplicated Percentage of total 1 75.16879085600691 41.08318696828771 2 13.761346279274072 15.042412035329104 3 4.590632048433236 7.526972002636318 4 2.119961207876652 4.634623581244083 5 1.1749426987232252 3.2107999888635574 6 0.6842717965408197 2.2439152606509802 7 0.4662687398580406 1.7838605515985235 8 0.33467408760743966 1.4633177368470383 9 0.24310372305167186 1.1958058701989212 >10 1.3148650846537586 13.100704203404643 >50 0.09769647302907494 3.6890873434362565 >100 0.04105332030291637 3.7766473336447697 >500 0.0017952634817726167 0.6480457434156864 >1k 5.984211605908722E-4 0.6006213804423977 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2764 0.1806471009212087 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2269 0.14829532271715723 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1556 0.1016956906777861 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.53571276849525E-5 0.0 3 0.0 0.0 0.0 6.53571276849525E-5 0.0 4 0.0 0.0 0.0 6.53571276849525E-5 0.0 5 0.0 0.0 0.0 6.53571276849525E-5 0.0 6 0.0 0.0 0.0 6.53571276849525E-5 0.0 7 0.0 0.0 0.0 6.53571276849525E-5 0.0 8 0.0 0.0 0.0 6.53571276849525E-5 0.0 9 0.0 0.0 0.0 6.53571276849525E-5 0.0 10-11 0.0 0.0 0.0 6.53571276849525E-5 0.0 12-13 0.0 0.0 0.0 1.30714255369905E-4 0.0 14-15 0.0 0.0 0.0 2.2874994689733375E-4 6.53571276849525E-5 16-17 0.0 0.0 0.0 3.92142766109715E-4 6.53571276849525E-5 18-19 9.803569152742875E-5 6.53571276849525E-5 0.0 4.901784576371438E-4 6.53571276849525E-5 20-21 1.960713830548575E-4 6.53571276849525E-5 0.0 7.189284045344775E-4 6.53571276849525E-5 22-23 1.960713830548575E-4 6.53571276849525E-5 0.0 0.0010130354791167639 9.803569152742875E-5 24-25 1.960713830548575E-4 6.53571276849525E-5 0.0 0.0016012496282813361 1.30714255369905E-4 26-27 1.960713830548575E-4 6.53571276849525E-5 0.0 0.0018953567028636225 1.30714255369905E-4 28-29 4.2482132995219125E-4 6.53571276849525E-5 0.0 0.002320178032815814 1.30714255369905E-4 30-31 4.574998937946675E-4 6.53571276849525E-5 0.0 0.0037253562780422923 1.30714255369905E-4 32-33 4.574998937946675E-4 6.53571276849525E-5 0.0 0.006601069896180202 1.30714255369905E-4 34-35 5.555355853220963E-4 6.53571276849525E-5 0.0 0.011176068834126878 1.30714255369905E-4 36-37 5.882141491645725E-4 6.53571276849525E-5 0.0 0.018201960060259273 1.30714255369905E-4 38-39 6.53571276849525E-4 6.53571276849525E-5 0.0 0.02960677884128348 1.30714255369905E-4 40-41 6.53571276849525E-4 6.53571276849525E-5 0.0 0.043887311240445606 1.30714255369905E-4 42-43 6.53571276849525E-4 6.53571276849525E-5 0.0 0.059605700448676684 1.30714255369905E-4 44-45 6.53571276849525E-4 6.53571276849525E-5 0.0 0.07460516125237328 1.30714255369905E-4 46-47 7.189284045344775E-4 6.53571276849525E-5 0.0 0.09434301381322893 1.30714255369905E-4 48-49 7.8428553221943E-4 6.53571276849525E-5 0.0 0.11774086552444193 1.30714255369905E-4 50-51 7.8428553221943E-4 6.53571276849525E-5 0.0 0.14332818101310085 1.30714255369905E-4 52-53 7.8428553221943E-4 6.53571276849525E-5 0.0 0.16767371107574564 1.6339281921238125E-4 54-55 7.8428553221943E-4 1.30714255369905E-4 0.0 0.19525441895879558 1.960713830548575E-4 56-57 8.496426599043825E-4 1.960713830548575E-4 0.0 0.22953423242955318 1.960713830548575E-4 58-59 8.496426599043825E-4 1.960713830548575E-4 0.0 0.2678008306890929 1.960713830548575E-4 60-61 8.496426599043825E-4 1.960713830548575E-4 0.0 0.3092372496413528 1.960713830548575E-4 62-63 8.496426599043825E-4 2.6142851073981E-4 0.0 0.3531572394456408 2.2874994689733375E-4 64-65 8.496426599043825E-4 2.9410707458228625E-4 0.0 0.39965883579348455 2.6142851073981E-4 66-67 9.14999787589335E-4 3.267856384247625E-4 0.0 0.4536438232612553 2.6142851073981E-4 68-69 9.14999787589335E-4 3.267856384247625E-4 0.0 0.5166480943495495 2.6142851073981E-4 70-71 9.476783514318113E-4 3.267856384247625E-4 0.0 0.5880507563453601 2.6142851073981E-4 72-73 0.0010130354791167639 3.267856384247625E-4 0.0 0.6615121678632467 2.6142851073981E-4 74-75 0.0010783926068017161 3.267856384247625E-4 0.0 0.7424569705010604 2.6142851073981E-4 76-77 0.0011110711706441925 3.267856384247625E-4 0.0 0.8294473074497322 2.6142851073981E-4 78-79 0.0011437497344866689 3.267856384247625E-4 0.0 0.9304240697229837 2.6142851073981E-4 80-81 0.001437856809068955 3.267856384247625E-4 0.0 1.0468577926937268 2.6142851073981E-4 82-83 0.001437856809068955 3.267856384247625E-4 0.0 1.1657750865164977 2.6142851073981E-4 84-85 0.0015032139367539075 3.267856384247625E-4 0.0 1.285868808637598 2.6142851073981E-4 86-87 0.0016012496282813361 3.267856384247625E-4 0.0 1.4144589573577422 2.6142851073981E-4 88-89 0.0016339281921238125 3.267856384247625E-4 0.0 1.5619373159788372 2.6142851073981E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2280 0.0 44.431366 1 GTATCAA 3735 0.0 38.583084 1 TCAACGC 4675 0.0 30.440212 4 ATCAACG 4680 0.0 30.407692 3 TATCAAC 4775 0.0 29.80662 2 CAACGCA 4790 0.0 29.709393 5 GTACATG 5975 0.0 29.610786 1 TACATGG 5975 0.0 29.378433 2 GTACCTG 1580 0.0 28.897419 1 AACGCAG 5015 0.0 28.471058 6 GTATACG 85 6.360761E-4 27.976667 1 TACCTGG 1620 0.0 27.235336 2 ACATGGG 6220 0.0 26.768545 3 GTATAGG 455 0.0 25.086771 1 ACGCAGA 6085 0.0 23.230778 7 CATGGGG 3555 0.0 22.817316 4 CGCAGAG 6255 0.0 22.675241 8 GCAGAGT 6840 0.0 20.181105 9 CATGGGA 2805 0.0 20.124363 4 GTGGTAT 1375 0.0 19.715921 1 >>END_MODULE