FastQCFastQC Report
Thu 26 May 2016
SRR936325_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936325_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031103
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24300.11963942744410304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.037.7022171
GTATCAA41950.033.9667931
ATCAACG49700.028.283593
TCAACGC51000.027.5619564
GTACATG68700.027.287171
CAACGCA51350.027.1897585
TATCAAC53100.026.6515082
TACATGG70950.025.8835892
AACGCAG55050.025.790896
ACATGGG69450.025.6923353
TAACGCG500.001640973523.78318476-77
TACCTGG18750.023.4761492
GTACCTG19400.023.4255371
CATGGGG40650.022.2380454
ACGCAGA65650.021.4829857
CGCAGAG66400.020.9557428
AGTACAT46800.020.5303022
GAGTACA51600.020.2750841
GTGTAGC12700.019.755691
GTATAGG5600.018.5975061
Produced by FastQC (version 0.11.5)